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https://www.readbyqxmd.com/read/29474001/proteogenomic-analysis-to-identify-missing-proteins-from-haploid-cell-lines
#1
Seung-Eun Lee, JongKeon Song, Korbinian Bösl, André C Müller, Dijana Vitko, Keiryn L Bennett, Giulio Superti-Furga, Akhilesh Pandey, Richard K Kandasamy, Min-Sik Kim
Chromosome-centric Human Proteome Project aims at identifying and characterizing protein products encoded from all human protein-coding genes. As of early 2017, 19,837 protein-coding genes have been annotated in the neXtProt database including 2,691 missing proteins that have never been identified by mass spectrometry. Missing proteins may be low abundant in many cell types or expressed only in a few cell types in human body such as sperms in testis. In this study, we performed expression proteomics of two near haploid cell types such as HAP1 and KBM-7 to hunt for missing proteins...
February 23, 2018: Proteomics
https://www.readbyqxmd.com/read/29470852/high-quality-human-and-rat-spermatozoal-rna-isolation-for-functional-genomic-studies
#2
E Bianchi, A Stermer, K Boekelheide, M Sigman, S J Hall, G Reyes, E Dere, K Hwang
Sperm RNA is a sensitive monitoring endpoint for male reproductive toxicants, and a potential biomarker to assess male infertility and sperm quality. However, isolation of sperm RNA is a challenging procedure due to the heterogeneous population of cells present in the ejaculate, the low yield of RNA per spermatozoon, and the absence of 18S and 28S ribosomal RNA subunits. The unique biology of spermatozoa has created some uncertainty in the field about RNA isolation methods, indicating the need for rigorous quality control checks to ensure reproducibility of data generated from sperm RNA...
February 22, 2018: Andrology
https://www.readbyqxmd.com/read/29468755/impact-of-a-mild-scrotal-heating-on-sperm-chromosomal-abnormality-acrosin-activity-and-seminal-alpha-glucosidase-in-human-fertile-males
#3
M-H Zhang, L-P Zhai, Z-Y Fang, A-N Li, Y Qiu, Y-X Liu
The aim of this study was to observe sperm aneuploidy, DNA integrity, seminal alpha-glucosidase (NAG) and acrosin activity (AA) under testicular heat stress (SH). Spermatozoa were obtained from 30 healthy adult volunteers subjected to scrotal warming at 43°C for 30-40 min on two successive days per week for 3 months between February 2012 and September 2016. Aniline blue (AB), acridine orange (AO) staining, TUNEL assay and FISH analysis to evaluate sperm function, sperm DNA integrity and chromosomal abnormalities were carried on before, during and after SH...
February 22, 2018: Andrologia
https://www.readbyqxmd.com/read/29468520/impact-of-di-2-ethylhexyl-phthalate-metabolites-on-male-reproductive-function-a-systematic-review-of-human-evidence
#4
REVIEW
Birgit Bjerre Høyer, Virissa Lenters, Aleksander Giwercman, Bo A G Jönsson, Gunnar Toft, Karin S Hougaard, Jens Peter E Bonde, Ina Olmer Specht
PURPOSE OF REVIEW: The purpose of this review is to systematically review the literature linking di-2-ethylhexyl phthalate (DEHP) exposure with effects on reproductive health in adult males. RECENT FINDINGS: Thirty-three papers were included of which 28 were cross-sectional. Twenty-one papers investigated semen samples, 18 investigated reproductive hormones, and three studies investigated time to pregnancy. Studies revealed some but inconsistent indications that higher urinary DEHP metabolite levels are associated with an increase in the proportion of spermatozoa with damaged DNA and to a decrease in sperm concentration and motility...
February 21, 2018: Current Environmental Health Reports
https://www.readbyqxmd.com/read/29467291/epigenetic-alterations-in-longevity-regulators-reduced-life-span-and-exacerbated-aging-related-pathology-in-old-father-offspring-mice
#5
Kan Xie, Devon P Ryan, Brandon L Pearson, Kristin S Henzel, Frauke Neff, Ramon O Vidal, Magali Hennion, Isabelle Lehmann, Melvin Schleif, Susanne Schröder, Thure Adler, Birgit Rathkolb, Jan Rozman, Anna-Lena Schütz, Cornelia Prehn, Michel E Mickael, Marco Weiergräber, Jerzy Adamski, Dirk H Busch, Gerhard Ehninger, Anna Matynia, Walker S Jackson, Eckhard Wolf, Helmut Fuchs, Valerie Gailus-Durner, Stefan Bonn, Martin Hrabě de Angelis, Dan Ehninger
Advanced age is not only a major risk factor for a range of disorders within an aging individual but may also enhance susceptibility for disease in the next generation. In humans, advanced paternal age has been associated with increased risk for a number of diseases. Experiments in rodent models have provided initial evidence that paternal age can influence behavioral traits in offspring animals, but the overall scope and extent of paternal age effects on health and disease across the life span remain underexplored...
February 21, 2018: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29462624/human-sperm-swimming-in-a-high-viscosity-mucus-analogue
#6
Kenta Ishimoto, Hermes Gadêlha, Eamonn A Gaffney, David J Smith, Jackson Kirkman-Brown
Remarkably, mammalian sperm maintain a substantive proportion of their progressive swimming speed within highly viscous fluids, including those of the female reproductive tract. Here, we analyse the digital microscopy of a human sperm swimming in a highly viscous, weakly elastic mucus analogue. We exploit principal component analysis to simplify its flagellar beat pattern, from which boundary element calculations are used to determine the time-dependent flow field around the sperm cell. The sperm flow field is further approximated in terms of regularized point forces, and estimates of the mechanical power consumption are determined, for comparison with analogous low viscosity media studies...
February 17, 2018: Journal of Theoretical Biology
https://www.readbyqxmd.com/read/29460293/effect-of-the-sexual-abstinence-period-recommended-by-the-world-health-organization-on-clinical-outcomes-of-fresh-embryo-transfer-cycles-with-normal-ovarian-response-after-intracytoplasmic-sperm-injection
#7
J W Lee, J H Cha, S H Shin, H J Cha, J H Kim, C K Park, K A Pak, J S Yoon, S Y Park
This study was to investigate whether the sexual abstinence period (SAP) recommended by the World Health Organization (WHO) affects clinical outcomes. We compared the rate of clinical outcomes between 2-7 and ≥8 days of SAP in first fresh embryo transfer after intracytoplasmic sperm injection (ICSI) in groups of young maternal age (YMA: <38 years) and old maternal age (OMA: ≥38 years). We conducted a retrospective study of 449 first ICSI cycles with a normal ovarian response. SAP was identified before collecting the semen samples...
February 20, 2018: Andrologia
https://www.readbyqxmd.com/read/29458401/efficacy-and-safety-of-ding-kun-dan-for-female-infertility-patients-with-predicted-poor-ovarian-response-undergoing-in-vitro-fertilization-intracytoplasmic-sperm-injection-study-protocol-for-a-randomized-controlled-trial
#8
Saihua Ma, Ruihong Ma, Tian Xia, Masoud Afnan, Xueru Song, Fengqin Xu, Guimin Hao, Fangfang Zhu, Jingpei Han, Zhimei Zhao
BACKGROUND: Women undergoing in vitro fertilization (IVF)/intracytoplasmic sperm injection (ICSI) who have a predicted poor ovarian response (POR) present a challenge for reproductive medicine specialists. Traditional Chinese medicine (TCM) is commonly used in China for such patients, in the belief that it will improve the ovarian response and ultimately increase pregnancy rates. However, there is a lack of high-quality evidence about the effect of TCM on improving ovarian response in such patients...
February 20, 2018: Trials
https://www.readbyqxmd.com/read/29457367/sperm-morphological-normality-under-high-magnification-is-correlated-to-male-infertility-and-predicts-embryo-development
#9
B F Zanetti, D P A F Braga, R R Provenza, R C S Figueira, A Iaconelli, E Borges
Human sperm morphology has been described as an essential parameter for the diagnosis of male infertility and a prognostic indicator of natural or assisted pregnancies. Nevertheless, standard morphological assessment remains a subjective analysis and its impact on intracytoplasmic sperm injection (ICSI) is also of limited value. The objective of this prospective cohort study was to investigate whether motile sperm organelle morphology examination (MSOME) can improve semen analysis by better defining male infertility and providing a better prognosis for ICSI up to a year later...
February 18, 2018: Andrology
https://www.readbyqxmd.com/read/29457364/transmission-electron-microscopy-analysis-of-the-origin-and-incidence-of-sperm-intranuclear-cytoplasmic-retention-in-fertile-and-teratozoospermia-men
#10
W-J Zhu
The human sperm nucleus contains cytoplasm. However, the origin and incidence of human sperm intranuclear cytoplasmic retention (INCR) remain unknown. The objectives of this study were to observe the morphological origin of INCR within the seminiferous epithelium and investigate the incidence of INCR in fertile and teratozoospermia men using transmission electron microscopy (TEM). By TEM, INCR initially appeared in elongating round spermatid nuclei and varied in size, number, shape, content, location and distribution within sperm nuclei...
February 18, 2018: Andrology
https://www.readbyqxmd.com/read/29454799/identification-of-genomic-variants-causing-sperm-abnormalities-and-reduced-male-fertility
#11
REVIEW
Jeremy F Taylor, Robert D Schnabel, Peter Sutovsky
Whole genome sequencing has identified millions of bovine genetic variants; however, there is currently little understanding about which variants affect male fertility. It is imperative that we begin to link detrimental genetic variants to sperm phenotypes via the analysis of semen samples and measurement of fertility for bulls with alternate genotypes. Artificial insemination (AI) bulls provide a useful model system because of extensive fertility records, measured as sire conception rates (SCR). Genetic variants with moderate to large effects on fertility can be identified by sequencing the genomes of fertile and subfertile or infertile sires identified with high or low SCR as adult AI bulls or yearling bulls that failed Breeding Soundness Evaluation...
February 10, 2018: Animal Reproduction Science
https://www.readbyqxmd.com/read/29453196/genome-wide-association-study-identifies-erbb4-on-2q34-as-a-novel-locus-associated-with-sperm-motility-in-japanese-men
#12
Youichi Sato, Atsushi Tajima, Takehiro Sato, Shiari Nozawa, Miki Yoshiike, Issei Imoto, Aiko Yamauchi, Teruaki Iwamoto
BACKGROUND: The decrease in sperm motility has a potent influence on fertilisation. Sperm motility, represented as the percentage of motile sperm in ejaculated sperms, is influenced by lifestyle habits or environmental factors and by inherited factors. However, genetic factors contributing to individual differences in sperm motility remain unclear. To identify genetic factors that influence human sperm motility, we performed a genome-wide association study (GWAS) of sperm motility. METHODS: A two-stage GWAS was conducted using 811 Japanese men in a discovery stage, followed by a replication study using an additional 779 Japanese men...
February 16, 2018: Journal of Medical Genetics
https://www.readbyqxmd.com/read/29452353/a-diagnostic-germ-cell-score-for-immature-testicular-tissue-at-risk-of-germ-cell-loss
#13
L Heckmann, D Langenstroth-Röwer, T Pock, J Wistuba, J-B Stukenborg, M Zitzmann, S Kliesch, S Schlatt, N Neuhaus
STUDY QUESTION: Can a systematic scoring procedure provide crucial information on the status of highly heterogeneous immature human testicular tissues in the context of cryopreservation for fertility preservation? SUMMARY ANSWER: We developed a systematic histological score as a novel diagnostic tool which differentiates the patient cohort according to the status of germ cell differentiation and number of spermatogonia (normal, diminished and absent), and which could be relevant in the fertility clinic...
February 14, 2018: Human Reproduction
https://www.readbyqxmd.com/read/29449551/mutations-in-cfap43-and-cfap44-cause-male-infertility-and-flagellum-defects-in-trypanosoma-and-human
#14
Charles Coutton, Alexandra S Vargas, Amir Amiri-Yekta, Zine-Eddine Kherraf, Selima Fourati Ben Mustapha, Pauline Le Tanno, Clémentine Wambergue-Legrand, Thomas Karaouzène, Guillaume Martinez, Serge Crouzy, Abbas Daneshipour, Seyedeh Hanieh Hosseini, Valérie Mitchell, Lazhar Halouani, Ouafi Marrakchi, Mounir Makni, Habib Latrous, Mahmoud Kharouf, Jean-François Deleuze, Anne Boland, Sylviane Hennebicq, Véronique Satre, Pierre-Simon Jouk, Nicolas Thierry-Mieg, Beatrice Conne, Denis Dacheux, Nicolas Landrein, Alain Schmitt, Laurence Stouvenel, Patrick Lorès, Elma El Khouri, Serge P Bottari, Julien Fauré, Jean-Philippe Wolf, Karin Pernet-Gallay, Jessica Escoffier, Hamid Gourabi, Derrick R Robinson, Serge Nef, Emmanuel Dulioust, Raoudha Zouari, Mélanie Bonhivers, Aminata Touré, Christophe Arnoult, Pierre F Ray
Spermatogenesis defects concern millions of men worldwide, yet the vast majority remains undiagnosed. Here we report men with primary infertility due to multiple morphological abnormalities of the sperm flagella with severe disorganization of the sperm axoneme, a microtubule-based structure highly conserved throughout evolution. Whole-exome sequencing was performed on 78 patients allowing the identification of 22 men with bi-allelic mutations in DNAH1 (n = 6), CFAP43 (n = 10), and CFAP44 (n = 6). CRISPR/Cas9 created homozygous CFAP43/44 male mice that were infertile and presented severe flagellar defects confirming the human genetic results...
February 15, 2018: Nature Communications
https://www.readbyqxmd.com/read/29449095/relationships-between-bacteriospermia-dna-integrity-nuclear-protamine-alteration-sperm-quality-and-icsi-outcome
#15
Ali Zeyad, Mohammed Hamad, Houda Amor, Mohamad Eid Hammadeh
The Aim of this study was to evaluate the effects of bacteriospermia on human sperm parameters, nuclear protamines, DNA integrity and ICSI outcome in patients enrolled for ICSI treatment. 84 unselected couples consulting in infertility and obstetrics clinic and enrolled for ICSI treatment were included in this study. The semen specimens were screened bacteriologically; semen and sperm parameters were also evaluated according to WHO guidelines. DNA integrity, protamines concentration and protamine deficiency were estimated by TUNEL assay, AU-PAGE and Chromomycin (CMA3) respectively...
February 12, 2018: Reproductive Biology
https://www.readbyqxmd.com/read/29449081/urinary-concentrations-of-benzophenone-type-ultra-violet-light-filters-and-reproductive-parameters-in-young-men
#16
Evdochia Adoamnei, Jaime Mendiola, Miriam Moñino-García, Fernando Vela-Soria, Luz M Iribarne-Durán, Mariana F Fernández, Nicolás Olea, Niels Jørgensen, Shanna H Swan, Alberto M Torres-Cantero
BACKGROUND: Benzophenone (BP)-type ultraviolet (UV) light filters are chemicals frequently added to personal care products, insect repellents, sunscreens, and beverage and food packaging to diminish the harmful effects of UV sunlight on human skin or foodstuffs. BP-type UV filters have shown negative effects on male reproduction function in in vitro and animal models, but human epidemiologic studies are limited. The goal of this study was to examine associations between urinary concentrations of BP-type UV filters and semen quality and reproductive hormone levels...
February 9, 2018: International Journal of Hygiene and Environmental Health
https://www.readbyqxmd.com/read/29449037/impact-of-light-irradiation-on-preservation-and-function-of-mammalian-spermatozoa
#17
REVIEW
Marc Yeste, Miriam Castillo-Martín, Sergi Bonet, Joan E Rodríguez-Gil
Light irradiation has been demonstrated to exert positive effects on gametes, and particularly on sperm. In effect, a high number of studies conducted in several species, including humans, mice, pigs, cattle and sheep, and using different light sources (such as lasers and light-emitting diodes) have demonstrated that photo-stimulation increases sperm motility. In addition, other works have shown that sperm fertilizing ability both in vitro and in vivo can be increased following light irradiation; there are also some evidences pointing out to an extend of lifespan of preserved semen...
February 9, 2018: Animal Reproduction Science
https://www.readbyqxmd.com/read/29448847/effect-of-early-cumulus-cells-removal-and-early-rescue-icsi-on-pregnancy-outcomes-in-high-risk-patients-of-fertilization-failure
#18
Yuxia He, Haiying Liu, Haiyan Zheng, Li Li, Xin Fu, Jianqiao Liu
OBJECTIVE: The objective of this retrospective study was to evaluate clinical outcomes of early cumulus cells removal and early rescue intracytoplasmic sperm injection (ICSI) in high-risk patients of fertilization failure during human in vitro fertilization (IVF). METHODS: A total of 5,518 patients were enrolled between January 2014 to December 2016. Of which 505 couples performed short insemination with >30% fertilization rate were included in short-term IVF group, 102 patients detected total fertilization failure (TFF) were treated with early rescue ICSI (R-ICSI group), and 4911 couples underwent conventional IVF with overnight co-incubation of gametes (traditional IVF group)...
February 15, 2018: Gynecological Endocrinology
https://www.readbyqxmd.com/read/29447380/sirtuins-in-gamete-biology-and-reproductive-physiology-emerging-roles-and-therapeutic-potential-in-female-and-male-infertility
#19
Carla Tatone, Giovanna Di Emidio, Arcangelo Barbonetti, Gaspare Carta, Alberto M Luciano, Stefano Falone, Fernanda Amicarelli
BACKGROUND: Sirtuins (SIRT1-7) are a family of NAD+-dependent deacetylases that catalyze post-translational modifications of proteins. Together, they respond to metabolic challenges, inflammatory signals or hypoxic/oxidative stress, and are associated with aging and longevity. The role of Sirtuins in the regulation of fertility emerged in 2003 when a defective reproductive phenotype was observed in SIRT1-null mice. Although studies on Sirtuins in reproductive biology have been increasing in the last years, a recent comprehensive update on this issue is still lacking...
February 13, 2018: Human Reproduction Update
https://www.readbyqxmd.com/read/29447173/dynamic-changes-of-setd2-a-histone-h3k36-methyltransferase-in-porcine-oocytes-ivf-and-scnt-embryos
#20
Yun Fei Diao, Tao Lin, Xiaoxia Li, Reza K Oqani, Jae Eun Lee, So Yeon Kim, Dong Il Jin
SETD2 (SET domain containing protein 2) acts as a histone H3 lysine 36 (H3K36)-specific methyltransferase and may play important roles in active gene transcription in human cells. However, its expression and role in porcine oocytes and preimplantation embryos are not well understood. Here, we used immunofluorescence and laser scanning confocal microscopy to examine SETD2 expression in porcine fetal fibroblasts, oocytes, and preimplantation embryos derived from in vitro fertilization (IVF), parthenogenetic activation (PA), and somatic cell nuclear transfer (SCNT)...
2018: PloS One
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