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https://www.readbyqxmd.com/read/28334623/rapid-on-chip-apoptosis-assay-on-human-carcinoma-cells-based-on-annexin-v-quantum-dot-probes
#1
Helena Montón, Mariana Medina-Sánchez, Joan Antoni Soler, Andrzej Chałupniak, Carme Nogués, Arben Merkoçi
Despite all the efforts made over years to study the cancer expression and the metastasis event, there is not a clear understanding of its origins and effective treatment. Therefore, more specialized and rapid techniques are required for studying cell behaviour under different drug-based treatments. Here we present a quantum dot signalling-based cell assay carried out in a segmental microfluidic device that allows studying the effect of anti-cancer drugs in cultured cell lines by monitoring phosphatidylserine translocation that occurs in early apoptosis...
March 18, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28325610/depositional-history-and-inventories-of-polychlorinated-biphenyls-pcbs-in-sediment-cores-from-an-antarctic-specially-managed-area-admiralty-bay-king-george-island
#2
Tatiane Combi, César C Martins, Satie Taniguchi, Juliana Leonel, Rafael André Lourenço, Rosalinda Carmela Montone
Temporal patterns, fluxes and inventories of polychlorinated biphenyls (PCBs) were assessed in nine sediment cores collected from selected areas of Admiralty Bay off the Antarctic Peninsula. Concentrations of total PCBs were low, but slightly higher in comparison to low-impacted, remote environments in the world, ranging from below the detection limit to 11.9ngg(-1) in dry weight. PCB concentrations and inventories suggest a possible minor influence related to the nearby logistic activities, especially in the sediment core collected close to the Ferraz Station...
March 18, 2017: Marine Pollution Bulletin
https://www.readbyqxmd.com/read/28318865/anterior-vs-posterior-periarticular-multimodal-drug-injections-a-randomized-controlled-trial-in-simultaneous-bilateral-total-knee-arthroplasty
#3
Artit Laoruengthana, Piti Rattanaprichavej, Supachok Rasamimongkol, Monton Galassi
BACKGROUND: Currently, various techniques are used to overcome postoperative pain after total knee arthroplasty. A local analgesic infiltration with periarticular multimodal drug injection (PMDI) is favorable because of its simplicity, safety, and efficacy. The present study compared the efficacy of a PMDI at the anterior vs posterior compartments. METHODS: Forty-six patients were randomized to receive the PMDI at either the anterior or posterior compartment, with the contralateral knee receiving the PMDI at the opposite compartment...
February 24, 2017: Journal of Arthroplasty
https://www.readbyqxmd.com/read/28297585/electromechanical-conditioning-of-adult-progenitor-cells-improves-recovery-of-cardiac-function-after-myocardial-infarction
#4
Aida Llucià-Valldeperas, Carolina Soler-Botija, Carolina Gálvez-Montón, Santiago Roura, Cristina Prat-Vidal, Isaac Perea-Gil, Benjamin Sanchez, Ramon Bragos, Gordana Vunjak-Novakovic, Antoni Bayes-Genis
Cardiac cells are subjected to mechanical and electrical forces, which regulate gene expression and cellular function. Therefore, in vitro electromechanical stimuli could benefit further integration of therapeutic cells into the myocardium. Our goals were (a) to study the viability of a tissue-engineered construct with cardiac adipose tissue-derived progenitor cells (cardiac ATDPCs) and (b) to examine the effect of electromechanically stimulated cardiac ATDPCs within a myocardial infarction (MI) model in mice for the first time...
March 2017: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/28267217/comparison-of-routinely-used-intestinal-biopsy-forceps-in-dogs-an-ex-vivo-histopathological-approach
#5
J Dahan, M O Semin, C Monton, S Amiriantz, D Concordet, I Raymond-Letron, O Dossin
OBJECTIVES: To compare the quality of duodenal and ileal samples obtained with different biopsy forceps. METHODS: Fifteen dogs were included in a prospective ex vivo study. After euthanasia, the duodenum and the ileum were sampled with four different forceps and evaluated according to a standardised scoring system. The biopsy forceps evaluated had alligator jaws or cups with smooth edge with or without a needle. RESULTS: The global quality of the biopsies was better in the ileum that in the duodenum regardless of the biopsy forceps...
March 2017: Journal of Small Animal Practice
https://www.readbyqxmd.com/read/28214080/clinical-outcome-and-correlates-of-coronary-microvascular-obstruction-in-latecomers-after-acute-myocardial-infarction
#6
Rocco A Montone, Giampaolo Niccoli, Silvia Minelli, Francesco Fracassi, Vincenzo Vetrugno, Cristina Aurigemma, Francesco Burzotta, Italo Porto, Carlo Trani, Filippo Crea
AIMS: Microvascular obstruction (MVO) is associated with a worse prognosis in patients with ST-elevation myocardial infarction (STEMI) treated by primary percutaneous coronary intervention (PCI). However, data about incidence, clinical outcome and correlates of MVO in latecomers after STEMI are still lacking. METHODS: We prospectively enrolled consecutive patients that were latecomers after STEMI (symptoms onset >12h) undergoing PCI. We performed an angiographic analysis to assess the occurrence of MVO [defined as TIMI flow grade ≤2 or 3 with a myocardial blush grade <2]...
February 12, 2017: International Journal of Cardiology
https://www.readbyqxmd.com/read/28202591/non-classical-phase-diagram-for-virus-bacterial-coevolution-mediated-by-clustered-regularly-interspaced-short-palindromic-repeats
#7
Pu Han, Michael W Deem
CRISPR is a newly discovered prokaryotic immune system. Bacteria and archaea with this system incorporate genetic material from invading viruses into their genomes, providing protection against future infection by similar viruses. The condition for coexistence of prokaryots and viruses is an interesting problem in evolutionary biology. In this work, we show an intriguing phase diagram of the virus extinction probability, which is more complex than that of the classical predator-prey model. As the CRISPR incorporates genetic material, viruses are under pressure to evolve to escape recognition by CRISPR...
February 2017: Journal of the Royal Society, Interface
https://www.readbyqxmd.com/read/28191775/noninvasive-assessment-of-an-engineered-bioactive-graft-in-myocardial-infarction-impact-on-cardiac-function-and-scar-healing
#8
Carolina Gálvez-Montón, Ramon Bragós, Carolina Soler-Botija, Idoia Díaz-Güemes, Cristina Prat-Vidal, Verónica Crisóstomo, Francisco M Sánchez-Margallo, Aida Llucià-Valldeperas, Paco Bogónez-Franco, Isaac Perea-Gil, Santiago Roura, Antoni Bayes-Genis
Cardiac tissue engineering, which combines cells and biomaterials, is promising for limiting the sequelae of myocardial infarction (MI). We assessed myocardial function and scar evolution after implanting an engineered bioactive impedance graft (EBIG) in a swine MI model. The EBIG comprises a scaffold of decellularized human pericardium, green fluorescent protein-labeled porcine adipose tissue-derived progenitor cells (pATPCs), and a customized-design electrical impedance spectroscopy (EIS) monitoring system...
February 2017: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/28153130/inflammatory-and-infectious-lesions-of-the-sinonasal-tract
#9
REVIEW
Kathleen T Montone, Virginia A LiVolsi
The sinonasal tract is frequently affected by nonneoplastic inflammatory diseases. Inflammatory lesions of the sinonasal tract can be divided into 3 main categories: chronic rhinosinusitis, which encompasses a heterogeneous group of entities, all of which result in mucosal inflammation with or without polyps-eosinophils; infectious diseases; and autoimmune diseases and vasculitides, which can result in midline necrosis and facial deformities. This article reviews the common inflammatory lesions of the sinonasal tract with emphasis on infectious diseases, vasculitis, iatrogenic, and diseases of unknown cause...
March 2017: Surgical Pathology Clinics
https://www.readbyqxmd.com/read/28145039/procedural-and-30-day-clinical-outcomes-following-transcatheter-aortic-valve-replacement-with-lotus-valve-results-of-the-relevant-study
#10
Rocco A Montone, Luca Testa, Chiara Fraccaro, Matteo Montorfano, Fausto Castriota, Roberto Nerla, Marco Angelillis, Maurizio Tusa, Francesco Giannini, Andrea Garatti, Giuseppe Tarantini, Anna Sonia Petronio, Nedy Brambilla, Francesco Bedogni
OBJECTIVES: We report procedural and 30-day clinical outcomes following transcatheter aortic valve replacement (TAVR) with Lotus Valve system in a high-risk population. BACKGROUND: Lotus valve is a second-generation TAVR fully repositionable and retrievable device. RELEVANT (REgistry of Lotus valvE for treatment of aortic VAlve steNosis with Tavr) study is an Italian prospective multicentre registry. METHODS: Five major centers performing TAVR using Lotus participated...
February 1, 2017: Catheterization and Cardiovascular Interventions
https://www.readbyqxmd.com/read/28116374/a-single-molecule-fret-sensor-for-monitoring-dna-synthesis-in-real-time
#11
Carel Fijen, Alejandro Montón Silva, Alejandro Hochkoeppler, Johannes Hohlbein
We developed a versatile DNA assay and framework for monitoring polymerization of DNA in real time and at the single-molecule level. The assay consists of an acceptor labelled DNA primer annealed to a DNA template that is labelled on its single stranded, downstream overhang with a donor fluorophore. Upon extension of the primer using a DNA polymerase, the overhang of the template alters its conformation from a random coil to the canonical structure of double stranded DNA. This conformational change increases the distance between the donor and the acceptor fluorophore and can be detected as a decrease in the Förster resonance energy transfer (FRET) efficiency between both fluorophores...
January 24, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28104791/percutaneous-treatment-of-an-iatrogenic-pseudoaneurism-of-the-aortic-valsalva-sinus
#12
Rocco A Montone, Francesco Bedogni, Federico De Marco
No abstract text is available yet for this article.
January 18, 2017: European Heart Journal
https://www.readbyqxmd.com/read/28042333/nanosized-ucmsc-derived-extracellular-vesicles-but-not-conditioned-medium-exclusively-inhibit-the-inflammatory-response-of-stimulated-t-cells-implications-for-nanomedicine
#13
Marta Monguió-Tortajada, Santiago Roura, Carolina Gálvez-Montón, Josep Maria Pujal, Gemma Aran, Lucía Sanjurjo, Marcel la Franquesa, Maria-Rosa Sarrias, Antoni Bayes-Genis, Francesc E Borràs
Undesired immune responses have drastically hampered outcomes after allogeneic organ transplantation and cell therapy, and also lead to inflammatory diseases and autoimmunity. Umbilical cord mesenchymal stem cells (UCMSCs) have powerful regenerative and immunomodulatory potential, and their secreted extracellular vesicles (EVs) are envisaged as a promising natural source of nanoparticles to increase outcomes in organ transplantation and control inflammatory diseases. However, poor EV preparations containing highly-abundant soluble proteins may mask genuine vesicular-associated functions and provide misleading data...
2017: Theranostics
https://www.readbyqxmd.com/read/28004360/minimally-invasive-adipose-graft-transposition-procedure
#14
Carolina Gálvez-Montón, Paloma Gastelurrutia, Idoia Diaz-Güemes, Francisco M Sanchez-Margallo, Antoni Bayes-Genis
Recently, a new surgical technique to rescue infarcted myocardium (AGTP) has already tested (NCT01473433, AdiFLAP Trial). Here, we present the new minimally invasive AGTP (mi-AGTP) by thoracoscopy.
February 2017: Journal of Cardiovascular Translational Research
https://www.readbyqxmd.com/read/27998847/the-failing-right-heart-implications-and-evolution-in-high-risk-patients-undergoing-transcatheter-aortic-valve-implantation
#15
Luca Testa, Azeem Latib, Federico De Marco, Marco De Carlo, Claudia Fiorina, Marco Barbanti, Rocco A Montone, Mauro Agnifili, Anna Sonia Petronio, Federica Ettori, Silvio Klugmann, Corrado Tamburino, Nedy Brambilla, Antonio Colombo, Francesco Bedogni
AIMS: Right ventricular dysfunction (RVdy) is negatively associated with survival after left heart valve surgery. It is unclear whether RVdy has the same impact in patients undergoing transcatheter aortic valve implantation (TAVI). We sought to evaluate the prognostic impact of different grades of RVdy on TAVI, with and without concomitant left ventricular dysfunction (LVdy), and the possible impact of TAVI on RVdy. METHODS AND RESULTS: Among 870 consecutive patients with severe symptomatic aortic stenosis undergoing TAVI, 226 patients (26%) presented with a concomitant diagnosis of RVdy...
December 20, 2016: EuroIntervention
https://www.readbyqxmd.com/read/27983691/evaluation-of-google-glass-technical-limitations-on-their-integration-in-medical-systems
#16
Antonio Martinez-Millana, Jose-Luis Bayo-Monton, Aroa Lizondo, Carlos Fernandez-Llatas, Vicente Traver
Google Glass is a wearable sensor presented to facilitate access to information and assist while performing complex tasks. Despite the withdrawal of Google in supporting the product, today there are multiple applications and much research analyzing the potential impact of this technology in different fields of medicine. Google Glass satisfies the need of managing and having rapid access to real-time information in different health care scenarios. Among the most common applications are access to electronic medical records, display monitorizations, decision support and remote consultation in specialties ranging from ophthalmology to surgery and teaching...
December 15, 2016: Sensors
https://www.readbyqxmd.com/read/27931198/fdg-pet-parameters-predicting-mediastinal-malignancy-in-lung-cancer
#17
M Serra Fortuny, M Gallego, Ll Berna, C Montón, L Vigil, M J Masdeu, A Fernández-Villar, M I Botana, R Cordovilla, R García-Luján, E Cases, E Monsó
BACKGROUND: Staging of mediastinal lymph nodes in non-small cell lung cancer (NSCLC) is mandatory. The maximum Standard Uptake Value (SUVmax) obtained using F-18 fluorodeoxyglucose positron emission tomography (FDG-PET) is the best non-invasive technique available for this evaluation, but its performance varies from center to center. The aim of the present study was to identify FDG-PET predictors of mediastinal malignancy that are able to minimize intercenter variability and improve the selection of subsequent staging procedures...
December 8, 2016: BMC Pulmonary Medicine
https://www.readbyqxmd.com/read/27930761/transoral-robotic-surgery-assisted-endoscopy-with-primary-site-detection-and-treatment-in-occult-mucosal-primaries
#18
Kyle M Hatten, Bert W O'Malley, Andres M Bur, Mihir R Patel, Christopher H Rassekh, Jason G Newman, Steven B Cannady, Ara A Chalian, Benjamin L Hodnett, Alexander Lin, John N Lukens, Roger B Cohen, Joshua M Bauml, Kathleen T Montone, Virginia A Livolsi, Gregory S Weinstein
Importance: Management of cervical lymph node metastasis without a known primary tumor is a diagnostic and treatment challenge for head and neck oncologists. Identification of the occult mucosal primary tumor minimizes the morbidity of treatment. Objective: To analyze the role of transoral robotic surgery (TORS) in facilitating the identification of a primary tumor site for patients presenting with squamous cell carcinoma of unknown primary (CUP). In addition, we assessed treatment deintensification by determining the number of patients who did not undergo definitive radiation therapy and chemotherapy...
March 1, 2017: JAMA Otolaryngology—Head & Neck Surgery
https://www.readbyqxmd.com/read/27875711/corrigendum-to-external-conditions-inversely-change-the-rna-polymerase-ii-elongation-rate-and-density-in-yeast-biochim-biophys-acta-2013-nov-1829-11-1248-1255
#19
Ana Miguel, Fernando Montón, Tianlu Li, Fernando Gómez-Herreros, Sebastián Chávez, Paula Alepuz, José E Pérez-Ortín
No abstract text is available yet for this article.
December 24, 2016: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/27799762/c-reactive-protein-in-outpatients-with-acute-exacerbation-of-copd-its-relationship-with-microbial-etiology-and-severity
#20
Miguel Gallego, Xavier Pomares, Silvia Capilla, Maria Angeles Marcos, David Suárez, Eduard Monsó, Concepción Montón
BACKGROUND: C-reactive protein (CRP) measurement has proven valuable for detecting exacerbations, but its usefulness in predicting etiology remains controversial. Likewise, its potential value as a marker of severity, which is well established in patients with pneumonia, remains unproven in chronic obstructive pulmonary disease (COPD) exacerbations. METHODS: A cohort study of 118 patients with severe COPD and acute infectious exacerbations were included and followed up over 1 year...
2016: International Journal of Chronic Obstructive Pulmonary Disease
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