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Jean-Emmanuel Hugonnet, Dominique Mengin-Lecreulx, Alejandro Monton, Tanneke den Blaauwen, Etienne Carbonnelle, Carole Veckerlé, Yves Brun, Michael van Nieuwenhze, Christiane Bouchier, Kuyek Tu, Louis B Rice, Michel Arthur
The target of β-lactam antibiotics is the D,D-transpeptidase activity of penicillin-binding proteins (PBPs) for synthesis of 4→3 cross-links in the peptidoglycan of bacterial cell walls. Unusual 3→3 cross-links formed by L,D-transpeptidases were first detected in Escherichia coli more than four decades ago, however no phenotype has previously been associated with their synthesis. Here we show that production of the L,D-transpeptidase YcbB in combination with elevated synthesis of the (p)ppGpp alarmone by RelA lead to full bypass of the D,D-transpeptidase activity of PBPs and to broad-spectrum β-lactam resistance...
October 21, 2016: ELife
Caio V Z Cipro, Rosalinda C Montone, Paco Bustamante
Mercury (Hg) can reach the environment through natural and human-related sources, threatening ecosystems all over the planet due to its well known deleterious effects. Therefore, Antarctic trophic webs, despite being relatively isolated, are not exempt of its influence. To evaluate Hg concentrations in an Antarctic ecosystem, different tissues from 2 species of invertebrates, 2 of fish, 8 of birds, 4 of pinnipeds and at least 5 of vegetation were investigated (n=176). For animals, values ranged from 0.018 to 48...
October 4, 2016: Marine Pollution Bulletin
Aida Llucià-Valldeperas, Carolina Soler-Botija, Carolina Gálvez-Montón, Santiago Roura, Cristina Prat-Vidal, Isaac Perea-Gil, Benjamin Sanchez, Ramon Bragos, Gordana Vunjak-Novakovic, Antoni Bayes-Genis
: : Cardiac cells are subjected to mechanical and electrical forces, which regulate gene expression and cellular function. Therefore, in vitro electromechanical stimuli could benefit further integration of therapeutic cells into the myocardium. Our goals were (a) to study the viability of a tissue-engineered construct with cardiac adipose tissue-derived progenitor cells (cardiac ATDPCs) and (b) to examine the effect of electromechanically stimulated cardiac ATDPCs within a myocardial infarction (MI) model in mice for the first time...
September 29, 2016: Stem Cells Translational Medicine
Carolina Gálvez-Montón, Ramon Bragós, Carolina Soler-Boti Ja, Idoia Díaz-Güemes, Cristina Prat-Vidal, Verónica Crisóstomo, Francisco M Sánchez-Margallo, Aida Llucià-Valldeperas, Paco Bogónez-Franco, Isaac Perea-Gil, Santiago Roura, Antoni Bayes-Genis
: : Cardiac tissue engineering, which combines cells and biomaterials, is promising for limiting the sequelae of myocardial infarction (MI). We assessed myocardial function and scar evolution after implanting an engineered bioactive impedance graft (EBIG) in a swine MI model. The EBIG comprises a scaffold of decellularized human pericardium, green fluorescent protein-labeled porcine adipose tissue-derived progenitor cells (pATPCs), and a customized-design electrical impedance spectroscopy (EIS) monitoring system...
September 2, 2016: Stem Cells Translational Medicine
Beatriz Toledano, Felipe Bisbal, Maria Luisa Camara, Carlos Labata, Elisabet Berastegui, Carolina Gálvez-Montón, Roger Villuendas, Axel Sarrias, Teresa Oliveres, Damià Pereferrer, Xavier Ruyra, Antoni Bayés-Genís
OBJECTIVES: In high-risk patients with severe aortic stenosis, aortic valve replacement (AVR) with a sutureless Perceval prosthesis (SU-AVR) can be performed instead of conventional AVR or transcatheter aortic valve implantation. Little data are available regarding postoperative conduction disorders after SU-AVR. We aimed to determine the incidence and predictors of new-onset complete atrioventricular block (NO-AVB) requiring permanent cardiac stimulation following SU-AVR. METHODS: We studied consecutive patients who underwent SU-AVR between 2013 and 2015...
August 29, 2016: Interactive Cardiovascular and Thoracic Surgery
Xiaomin Zhong, Lan Zheng, Jianfeng Shen, Dongmei Zhang, Minmin Xiong, Youyou Zhang, Xinhong He, Janos L Tanyi, Feng Yang, Kathleen T Montone, Xiaojun Chen, Congjian Xu, Andy P Xiang, Qihong Huang, Xiaowei Xu, Lin Zhang
Oncogenic KRAS contributes to malignant transformation, antiapoptosis, and metastasis in multiple human cancers, such as lung, colon, and pancreatic cancers and melanoma. MicroRNAs (miRNAs) are endogenous 18- to 25-nucleotide noncoding small RNAs that regulate gene expression in a sequence-specific manner via the degradation of target mRNAs or inhibition of protein translation. In the present study, using array-based miRNA profiling in IMR90 and MCF10A cells expressing oncogenic KRAS, we identified that the expression of the microRNA 200 (mir-200) family was suppressed by KRAS activation and that this suppression was mediated by the transcription factors JUN and SP1 in addition to ZEB1...
November 1, 2016: Molecular and Cellular Biology
Josilene da Silva, Satie Taniguchi, José Henrique Becker, Max Rondon Werneck, Rosalinda Carmela Montone
Organochlorines (OCs), such as pesticides and polychlorinated biphenyls (PCBs), are persistent, toxic and widely distributed through atmospheric transport and ocean currents. Few studies have been conducted on OCs in sea turtles, especially on the coast of Brazil. Chelonia mydas is the largest hard-shell sea turtle and is found tropical and subtropical regions in all oceans. The aim of the present study was to determine the occurrence of OCs in the green sea turtle (C. mydas). Fat, liver, kidney and muscle samples were collected from 27 juveniles found on the beach of the city of Ubatuba on the northern coast of the state of São Paulo, Brazil...
November 15, 2016: Marine Pollution Bulletin
Alysson Wagner Fernandes Duarte, Michel Rodrigo Zambrano Passarini, Tiago Palladino Delforno, Franciane Maria Pellizzari, Caio Vinicius Zecchin Cipro, Rosalinda Carmela Montone, Maria Virginia Petry, Jair Putzke, Luiz Henrique Rosa, Lara Durães Sette
Antarctic terrestrial ecosystems are largely dominated by lichens, while shallow coastal environments are mainly covered by macroalgae. The aim of this study was to isolate and to evaluate the diversity of yeasts in different species of macroalgae and lichens collected in South Shetland Islands, Antarctica. A total of 405 yeasts were recovered (205 from macroalgae and 200 from lichens). The yeast community from macroalgae was most diversity than the yeast community from lichen. The dominance index was similar for both substrates...
August 12, 2016: Environmental Microbiology Reports
Antoni Bayés-Genís, Carolina Gálvez-Montón, Santiago Roura
No abstract text is available yet for this article.
August 16, 2016: Journal of the American College of Cardiology
Nicole D Facompre, Kayla M Harmeyer, Xavier Sole, Sheheryar Kabraji, Zachary Belden, Varun Sahu, Kelly Whelan, Koji Tanaka, Gregory S Weinstein, Kathleen T Montone, Alexander Roesch, Phyllis A Gimotty, Meenhard Herlyn, Anil K Rustgi, Hiroshi Nakagawa, Sridhar Ramaswamy, Devraj Basu
The degree of heterogeneity among cancer stem cells (CSC) remains ill-defined and may hinder effective anti-CSC therapy. Evaluation of oral cancers for such heterogeneity identified two compartments within the CSC pool. One compartment was detected using a reporter for expression of the H3K4me3 demethylase JARID1B to isolate a JARID1B(high) fraction of cells with stem cell-like function. JARID1B(high) cells expressed oral CSC markers including CD44 and ALDH1 and showed increased PI3K pathway activation. They were distinguished from a fraction in a G0-like cell-cycle state characterized by low reactive oxygen species and suppressed PI3K/AKT signaling...
September 15, 2016: Cancer Research
Luca Testa, Azeem Latib, Rocco A Montone, Antonio Colombo, Francesco Bedogni
Bioresorbable vascular scaffolds (BVS) represent a promising novel approach for the treatment of coronary artery disease. BVS promise to address some of the well-known limitations of current drug-eluting stents, while providing a transient scaffolding of the vessel to prevent acute vessel closure/recoil. Drug elution by BVS prevents neointimal proliferation in a similar fashion to drug-eluting stents, and complete bioresorption is associated with late vessel lumen enlargement, plaque regression, and restoration of vasomotion...
July 2016: Circulation. Cardiovascular Interventions
Shuanzeng Wei, Virginia A LiVolsi, Kathleen T Montone, Jennifer J D Morrissette, Zubair W Baloch
Medullary thyroid carcinoma (MTC) harbors rearranged during transfection (RET) gene and rarely RAS gene mutations. The knowledge of the type of gene mutation in MTC is important to determine the treatment of the patients and the management of their family members. Targeted next-generation sequencing with a panel of 47 genes was performed in a total of 12 cases of sporadic (9/12) and hereditary MTC (3/12). Two of three hereditary MTCs had RET/C634R mutation, while the other one harbored two RET mutations (L790F and S649L)...
July 5, 2016: Endocrine Pathology
Rafael Trevisan, Fabrício Flores-Nunes, Euler S Dolores, Jacó J Mattos, Clei E Piazza, Sílvio T Sasaki, Satie Taniguchi, Rosalinda C Montone, Márcia C Bícego, Isis M M Dos Reis, Flávia L Zacchi, Bárbara N M Othero, Camila L V Bastolla, Danielle F Mello, Ana Paula M Fraga, Nestor Wendt, Guilherme Toledo-Silva, Guilherme Razzera, Alcir L Dafre, Cláudio M R de Melo, Adalto Bianchini, Maria R F Marques, Afonso C D Bainy
Urban sewage is a concerning issue worldwide, threatening both wildlife and human health. The present study investigated protein oxidation in Mangrove Oysters (Crassostrea brasiliana) exposed to seawater from Balneário Camboriú, an important tourist destination in Brazil that is affected by urban sewage. Oysters were exposed for 24 h to seawater collected close to Camboriú river (CAM1) or 1 kilometer away (CAM2). Seawater from an aquaculture laboratory was used as a reference. Local sewage input was marked by higher levels of coliforms, nitrogen, and phosphorus in seawater, as wells as polycyclic aromatic hydrocarbons (PAHs), polychlorinated biphenyls (PCBs), linear alkylbenzenes (LABs), and fecal steroid in sediments at CAM1...
July 1, 2016: Environmental Toxicology and Chemistry
Antoni Bayes-Genis, Paloma Gastelurrutia, Maria-Luisa Cámara, Albert Teis, Josep Lupón, Cinta Llibre, Elisabet Zamora, Xavier Alomar, Xavier Ruyra, Santiago Roura, Ana Revilla, José Alberto San Román, Carolina Gálvez-Montón
BACKGROUND: The present study evaluates the safety and efficacy of the Adipose Graft Transposition Procedure (AGTP) as a biological regenerative innovation for patients with a chronic myocardial scar. METHODS: This prospective, randomized single-center controlled study included 10 patients with established chronic transmural myocardial scars. Candidates for myocardial revascularization were randomly allocated into two treatment groups. In the control arm (n=5), the revascularizable area was treated with CABG and the non-revascularizable area was left untouched...
May 2016: EBioMedicine
Luca Testa, Azeem Latib, Federico De Marco, Marco De Carlo, Claudia Fiorina, Rocco Montone, Mauro Agnifili, Marco Barbanti, Anna Sonia Petronio, Giuseppe Biondi Zoccai, Federica Ettori, Silvio Klugmann, Corrado Tamburino, Nedy Brambilla, Antonio Colombo, Francesco Bedogni
BACKGROUND: Severe pulmonary hypertension (PH) is considered to negatively affect the outcome after transcatheter aortic valve replacement. However, a clear understanding of the pattern, evolution, and clinical impact of different grades of PH in this setting is lacking. METHODS AND RESULTS: A total of 990 consecutive patients were enrolled in 6 high-volume centers and analyzed as follows: group 1, systolic pulmonary artery pressure (sPAP) <40 mm Hg (346 patients; 35%); group 2, sPAP 40 to 60 mm Hg (426 patients; 43%); and group 3, sPAP >60 mm Hg (218 patients; 22%)...
June 2016: Circulation. Cardiovascular Interventions
Francisco Buitrago Ramírez, Ramón Ciurana Misol, Levy Chocrón Bentata, María Del Carmen Fernández Alonso, Javier García Campayo, Carmen Montón Franco, Jorge Luis Tizón García
No abstract text is available yet for this article.
June 2016: Atencion Primaria
Panwara Paritakul, Monton Wongwandee, Tanitra Tantitemit, Somphoch Pumipichet, Reg Dennick
BACKGROUND: An appropriate faculty development program is imperative for every medical school and should be tailored to suit the needs of individuals and the institution. OBJECTIVE: To measure the average level of confidence in each of the 12 roles of medical teachers and analyse if any of these factors: age, gender duration of teaching, and teaching in pre-clinical or clinical years has effect on the level of confidence in the teacher roles. MATERIAL AND METHOD: The online questionnaire invitations were sent via email to all 211 faculty members at Faculty of Medicine, Srinakharinwirot University, and 118 (55...
November 2015: Journal of the Medical Association of Thailand, Chotmaihet Thangphaet
Luca Testa, Azeem Latib, Rocco A Montone, Francesco Bedogni
Since the first transcatheter heart valve implantation in the pulmonary position in 2000 and in the aortic position in 2002, a large number of transcatheter heart valves have reached the clinical arena and thousands of high-risk patients have been treated successfully, in particular those with severe aortic stenosis. In contrast, the experience of transcatheter mitral valve repair or implantation started relatively more recently, and only a few devices are available at the moment. The aim of this review is to describe the different percutaneous systems for the treatment of mitral regurgitation...
August 2016: Journal of Thoracic and Cardiovascular Surgery
Fernanda I Colabuono, Stacy S Vander Pol, Kevin M Huncik, Satie Taniguchi, Maria V Petry, John R Kucklick, Rosalinda C Montone
Seabirds play an important role as top consumers in the food web and can be used as biomonitors of exposure to pollutants. Contamination studies involving non-destructive sampling methods are of considerable importance, allowing better evaluation of the levels of pollutants and their toxic effects. In the present study, organohalogen contaminants were analyzed in 113 blood samples from Southern Giant Petrel (Macronectes giganteus) adults and chicks collected in the austral summer of 2011/2012 and 2012/2013 from colonies on Elephant and Livingston Islands, South Shetland, Antarctica...
September 2016: Environmental Pollution
Aaron M Udager, Jonathan B McHugh, Bryan L Betz, Kathleen T Montone, Virginia A Livolsi, Raja R Seethala, Evgeny Yakirevich, O Hans Iwenofu, Bayardo Perez-Ordonez, Kathleen E DuRoss, Helmut C Weigelin, Megan S Lim, Kojo Sj Elenitoba-Johnson, Noah A Brown
Oncocytic sinonasal papillomas (OSPs) are benign tumours of the sinonasal tract, a subset of which are associated with synchronous or metachronous sinonasal squamous cell carcinoma (SNSCC). Activating EGFR mutations were recently identified in nearly 90% of inverted sinonasal papillomas (ISPs) - a related tumour with distinct morphology. EGFR mutations were, however, not found in OSP, suggesting that different molecular alterations drive the oncogenesis of these tumours. In this study, tissue from 51 cases of OSP and five cases of OSP-associated SNSCC was obtained retrospectively from six institutions...
August 2016: Journal of Pathology
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