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https://www.readbyqxmd.com/read/28468789/intracoronary-administration-of-allogeneic-adipose-tissue-derived-mesenchymal-stem-cells-improves-myocardial-perfusion-but-not-left-ventricle-function-in-a-translational-model-of-acute-myocardial-infarction
#1
Joaquim Bobi, Núria Solanes, Rodrigo Fernández-Jiménez, Carlos Galán-Arriola, Ana Paula Dantas, Leticia Fernández-Friera, Carolina Gálvez-Montón, Elisabet Rigol-Monzó, Jaume Agüero, José Ramírez, Mercè Roqué, Antoni Bayés-Genís, Javier Sánchez-González, Ana García-Álvarez, Manel Sabaté, Santiago Roura, Borja Ibáñez, Montserrat Rigol
BACKGROUND: Autologous adipose tissue-derived mesenchymal stem cells (ATMSCs) therapy is a promising strategy to improve post-myocardial infarction outcomes. In a porcine model of acute myocardial infarction, we studied the long-term effects and the mechanisms involved in allogeneic ATMSCs administration on myocardial performance. METHODS AND RESULTS: Thirty-eight pigs underwent 50 minutes of coronary occlusion; the study was completed in 33 pigs. After reperfusion, allogeneic ATMSCs or culture medium (vehicle) were intracoronarily administered...
May 3, 2017: Journal of the American Heart Association
https://www.readbyqxmd.com/read/28461746/copd-is-a-clear-risk-factor-for-increased-use-of-resources-and-adverse-outcomes-in-patients-undergoing-intervention-for-colorectal-cancer-a-nationwide-study-in-spain
#2
Marisa Baré, Concepción Montón, Laura Mora, Maximino Redondo, Marina Pont, Antonio Escobar, Cristina Sarasqueta, Nerea Fernández de Larrea, Eduardo Briones, Jose Maria Quintana
BACKGROUND: We hypothesized that patients undergoing surgery for colorectal cancer (CRC) with COPD as a comorbidity would consume more resources and have worse in-hospital outcomes than similar patients without COPD. Therefore, we compared different aspects of the care process and short-term outcomes in patients undergoing surgery for CRC, with and without COPD. METHODS: This was a prospective study and it included patients from 22 hospitals located in Spain - 472 patients with COPD and 2,276 patients without COPD undergoing surgery for CRC...
2017: International Journal of Chronic Obstructive Pulmonary Disease
https://www.readbyqxmd.com/read/28431835/variability-in-the-use-of-neuroimaging-techniques-for-diagnosis-and-follow-up-of-stroke-patients
#3
C Valcárcel-Nazco, D Alonso-Modino, F Montón-Álvarez, R Sabatel-Hernández, M S Pastor-Santoveña, P Mesa-Blanco, J C López-Fernández, P Serrano-Aguilar
INTRODUCTION AND OBJECTIVE: Imaging diagnosis is essential for treatment planning in stroke patients. However, use of these techniques varies due to uncertainty about their effectiveness. Our purpose was to describe the use of CT and MRI in stroke and transient ischaemic attack (TIA) over 5years in hospitals belonging to the Canary Islands Health Service and analyse interhospital variability based on routinely collected administrative data. PATIENTS AND METHOD: We gathered the minimum basic dataset (MBDS) from patients diagnosed with stroke or TIA between 2005 and 2010 in 4hospitals...
April 18, 2017: Neurología: Publicación Oficial de la Sociedad Española de Neurología
https://www.readbyqxmd.com/read/28430148/a-rapid-magnetic-solid-phase-extraction-method-followed-by-liquid-chromatography-tandem-mass-spectrometry-analysis-for-the-determination-of-mycotoxins-in-cereals
#4
Giorgia La Barbera, Anna Laura Capriotti, Chiara Cavaliere, Patrizia Foglia, Carmela Maria Montone, Riccardo Zenezini Chiozzi, Aldo Laganà
Mycotoxins can contaminate various food commodities, including cereals. Moreover, mycotoxins of different classes can co-contaminate food, increasing human health risk. Several analytical methods have been published in the literature dealing with mycotoxins determination in cereals. Nevertheless, in the present work, the aim was to propose an easy and effective system for the extraction of six of the main mycotoxins from corn meal and durum wheat flour, i.e., the main four aflatoxins, ochratoxin A, and the mycoestrogen zearalenone...
April 21, 2017: Toxins
https://www.readbyqxmd.com/read/28371507/reporting-of-fine-needle-aspiration-fna-specimens-of-salivary-gland-lesions-a-comprehensive-review
#5
REVIEW
Shuanzeng Wei, Lester J Layfield, Virginia A LiVolsi, Kathleen T Montone, Zubair W Baloch
Currently, there is no uniform classification scheme available for reporting of salivary gland fine-needle aspiration (FNA) specimens. Recently, an International group of pathologists has recommended a tiered classification scheme for reporting of salivary gland FNA results known as the "Milan System for Reporting Salivary Gland Cytopathology (MSRSGC)." We performed a comprehensive review of the published literature on FNA of salivary gland lesions by employing the diagnostic categories of the MSRSGC to evaluate their reliability in the management of salivary gland lesions...
March 31, 2017: Diagnostic Cytopathology
https://www.readbyqxmd.com/read/28360074/predicting-the-onset-of-hazardous-alcohol-drinking-in-primary-care-development-and-validation-of-a-simple-risk-algorithm
#6
Juan Ángel Bellón, Juan de Dios Luna, Michael King, Irwin Nazareth, Emma Motrico, María Josefa GildeGómez-Barragán, Francisco Torres-González, Carmen Montón-Franco, Marta Sánchez-Celaya, Miguel Ángel Díaz-Barreiros, Catalina Vicens, Patricia Moreno-Peral
BACKGROUND: Little is known about the risk of progressing to hazardous alcohol use in abstinent or low-risk drinkers. AIM: To develop and validate a simple brief risk algorithm for the onset of hazardous alcohol drinking (HAD) over 12 months for use in primary care. DESIGN AND SETTING: Prospective cohort study in 32 health centres from six Spanish provinces, with evaluations at baseline, 6 months, and 12 months. METHOD: Forty-one risk factors were measured and multilevel logistic regression and inverse probability weighting were used to build the risk algorithm...
April 2017: British Journal of General Practice: the Journal of the Royal College of General Practitioners
https://www.readbyqxmd.com/read/28334623/rapid-on-chip-apoptosis-assay-on-human-carcinoma-cells-based-on-annexin-v-quantum-dot-probes
#7
Helena Montón, Mariana Medina-Sánchez, Joan Antoni Soler, Andrzej Chałupniak, Carme Nogués, Arben Merkoçi
Despite all the efforts made over years to study the cancer expression and the metastasis event, there is not a clear understanding of its origins and effective treatment. Therefore, more specialized and rapid techniques are required for studying cell behaviour under different drug-based treatments. Here we present a quantum dot signalling-based cell assay carried out in a segmental microfluidic device that allows studying the effect of anti-cancer drugs in cultured cell lines by monitoring phosphatidylserine translocation that occurs in early apoptosis...
March 18, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28325610/depositional-history-and-inventories-of-polychlorinated-biphenyls-pcbs-in-sediment-cores-from-an-antarctic-specially-managed-area-admiralty-bay-king-george-island
#8
Tatiane Combi, César C Martins, Satie Taniguchi, Juliana Leonel, Rafael André Lourenço, Rosalinda Carmela Montone
Temporal patterns, fluxes and inventories of polychlorinated biphenyls (PCBs) were assessed in nine sediment cores collected from selected areas of Admiralty Bay off the Antarctic Peninsula. Concentrations of total PCBs were low, but slightly higher in comparison to low-impacted, remote environments in the world, ranging from below the detection limit to 11.9ngg(-1) in dry weight. PCB concentrations and inventories suggest a possible minor influence related to the nearby logistic activities, especially in the sediment core collected close to the Ferraz Station...
May 15, 2017: Marine Pollution Bulletin
https://www.readbyqxmd.com/read/28318865/anterior-vs-posterior-periarticular-multimodal-drug-injections-a-randomized-controlled-trial-in-simultaneous-bilateral-total-knee-arthroplasty
#9
Artit Laoruengthana, Piti Rattanaprichavej, Supachok Rasamimongkol, Monton Galassi
BACKGROUND: Currently, various techniques are used to overcome postoperative pain after total knee arthroplasty. A local analgesic infiltration with periarticular multimodal drug injection (PMDI) is favorable because of its simplicity, safety, and efficacy. The present study compared the efficacy of a PMDI at the anterior vs posterior compartments. METHODS: Forty-six patients were randomized to receive the PMDI at either the anterior or posterior compartment, with the contralateral knee receiving the PMDI at the opposite compartment...
February 24, 2017: Journal of Arthroplasty
https://www.readbyqxmd.com/read/28297585/electromechanical-conditioning-of-adult-progenitor-cells-improves-recovery-of-cardiac-function-after-myocardial-infarction
#10
Aida Llucià-Valldeperas, Carolina Soler-Botija, Carolina Gálvez-Montón, Santiago Roura, Cristina Prat-Vidal, Isaac Perea-Gil, Benjamin Sanchez, Ramon Bragos, Gordana Vunjak-Novakovic, Antoni Bayes-Genis
Cardiac cells are subjected to mechanical and electrical forces, which regulate gene expression and cellular function. Therefore, in vitro electromechanical stimuli could benefit further integration of therapeutic cells into the myocardium. Our goals were (a) to study the viability of a tissue-engineered construct with cardiac adipose tissue-derived progenitor cells (cardiac ATDPCs) and (b) to examine the effect of electromechanically stimulated cardiac ATDPCs within a myocardial infarction (MI) model in mice for the first time...
March 2017: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/28267217/comparison-of-routinely-used-intestinal-biopsy-forceps-in-dogs-an-ex-vivo-histopathological-approach
#11
J Dahan, M O Semin, C Monton, S Amiriantz, D Concordet, I Raymond-Letron, O Dossin
OBJECTIVES: To compare the quality of duodenal and ileal samples obtained with different biopsy forceps. METHODS: Fifteen dogs were included in a prospective ex vivo study. After euthanasia, the duodenum and the ileum were sampled with four different forceps and evaluated according to a standardised scoring system. The biopsy forceps evaluated had alligator jaws or cups with smooth edge with or without a needle. RESULTS: The global quality of the biopsies was better in the ileum that in the duodenum regardless of the biopsy forceps...
March 2017: Journal of Small Animal Practice
https://www.readbyqxmd.com/read/28214080/clinical-outcome-and-correlates-of-coronary-microvascular-obstruction-in-latecomers-after-acute-myocardial-infarction
#12
Rocco A Montone, Giampaolo Niccoli, Silvia Minelli, Francesco Fracassi, Vincenzo Vetrugno, Cristina Aurigemma, Francesco Burzotta, Italo Porto, Carlo Trani, Filippo Crea
AIMS: Microvascular obstruction (MVO) is associated with a worse prognosis in patients with ST-elevation myocardial infarction (STEMI) treated by primary percutaneous coronary intervention (PCI). However, data about incidence, clinical outcome and correlates of MVO in latecomers after STEMI are still lacking. METHODS: We prospectively enrolled consecutive patients that were latecomers after STEMI (symptoms onset >12h) undergoing PCI. We performed an angiographic analysis to assess the occurrence of MVO [defined as TIMI flow grade ≤2 or 3 with a myocardial blush grade <2]...
February 12, 2017: International Journal of Cardiology
https://www.readbyqxmd.com/read/28202591/non-classical-phase-diagram-for-virus-bacterial-coevolution-mediated-by-clustered-regularly-interspaced-short-palindromic-repeats
#13
Pu Han, Michael W Deem
CRISPR is a newly discovered prokaryotic immune system. Bacteria and archaea with this system incorporate genetic material from invading viruses into their genomes, providing protection against future infection by similar viruses. The condition for coexistence of prokaryots and viruses is an interesting problem in evolutionary biology. In this work, we show an intriguing phase diagram of the virus extinction probability, which is more complex than that of the classical predator-prey model. As the CRISPR incorporates genetic material, viruses are under pressure to evolve to escape recognition by CRISPR...
February 2017: Journal of the Royal Society, Interface
https://www.readbyqxmd.com/read/28191775/noninvasive-assessment-of-an-engineered-bioactive-graft-in-myocardial-infarction-impact-on-cardiac-function-and-scar-healing
#14
Carolina Gálvez-Montón, Ramon Bragós, Carolina Soler-Botija, Idoia Díaz-Güemes, Cristina Prat-Vidal, Verónica Crisóstomo, Francisco M Sánchez-Margallo, Aida Llucià-Valldeperas, Paco Bogónez-Franco, Isaac Perea-Gil, Santiago Roura, Antoni Bayes-Genis
Cardiac tissue engineering, which combines cells and biomaterials, is promising for limiting the sequelae of myocardial infarction (MI). We assessed myocardial function and scar evolution after implanting an engineered bioactive impedance graft (EBIG) in a swine MI model. The EBIG comprises a scaffold of decellularized human pericardium, green fluorescent protein-labeled porcine adipose tissue-derived progenitor cells (pATPCs), and a customized-design electrical impedance spectroscopy (EIS) monitoring system...
February 2017: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/28153130/inflammatory-and-infectious-lesions-of-the-sinonasal-tract
#15
REVIEW
Kathleen T Montone, Virginia A LiVolsi
The sinonasal tract is frequently affected by nonneoplastic inflammatory diseases. Inflammatory lesions of the sinonasal tract can be divided into 3 main categories: chronic rhinosinusitis, which encompasses a heterogeneous group of entities, all of which result in mucosal inflammation with or without polyps-eosinophils; infectious diseases; and autoimmune diseases and vasculitides, which can result in midline necrosis and facial deformities. This article reviews the common inflammatory lesions of the sinonasal tract with emphasis on infectious diseases, vasculitis, iatrogenic, and diseases of unknown cause...
March 2017: Surgical Pathology Clinics
https://www.readbyqxmd.com/read/28145039/procedural-and-30-day-clinical-outcomes-following-transcatheter-aortic-valve-replacement-with-lotus-valve-results-of-the-relevant-study
#16
Rocco A Montone, Luca Testa, Chiara Fraccaro, Matteo Montorfano, Fausto Castriota, Roberto Nerla, Marco Angelillis, Maurizio Tusa, Francesco Giannini, Andrea Garatti, Giuseppe Tarantini, Anna Sonia Petronio, Nedy Brambilla, Francesco Bedogni
OBJECTIVES: We report procedural and 30-day clinical outcomes following transcatheter aortic valve replacement (TAVR) with Lotus Valve system in a high-risk population. BACKGROUND: Lotus valve is a second-generation TAVR fully repositionable and retrievable device. RELEVANT (REgistry of Lotus valvE for treatment of aortic VAlve steNosis with Tavr) study is an Italian prospective multicentre registry. METHODS: Five major centers performing TAVR using Lotus participated...
February 1, 2017: Catheterization and Cardiovascular Interventions
https://www.readbyqxmd.com/read/28116374/a-single-molecule-fret-sensor-for-monitoring-dna-synthesis-in-real-time
#17
Carel Fijen, Alejandro Montón Silva, Alejandro Hochkoeppler, Johannes Hohlbein
We developed a versatile DNA assay and framework for monitoring polymerization of DNA in real time and at the single-molecule level. The assay consists of an acceptor labelled DNA primer annealed to a DNA template that is labelled on its single stranded, downstream overhang with a donor fluorophore. Upon extension of the primer using a DNA polymerase, the overhang of the template alters its conformation from a random coil to the canonical structure of double stranded DNA. This conformational change increases the distance between the donor and the acceptor fluorophore and can be detected as a decrease in the Förster resonance energy transfer (FRET) efficiency between both fluorophores...
January 24, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28104791/percutaneous-treatment-of-an-iatrogenic-pseudoaneurism-of-the-aortic-valsalva-sinus
#18
Rocco A Montone, Francesco Bedogni, Federico De Marco
No abstract text is available yet for this article.
January 18, 2017: European Heart Journal
https://www.readbyqxmd.com/read/28042333/nanosized-ucmsc-derived-extracellular-vesicles-but-not-conditioned-medium-exclusively-inhibit-the-inflammatory-response-of-stimulated-t-cells-implications-for-nanomedicine
#19
Marta Monguió-Tortajada, Santiago Roura, Carolina Gálvez-Montón, Josep Maria Pujal, Gemma Aran, Lucía Sanjurjo, Marcel la Franquesa, Maria-Rosa Sarrias, Antoni Bayes-Genis, Francesc E Borràs
Undesired immune responses have drastically hampered outcomes after allogeneic organ transplantation and cell therapy, and also lead to inflammatory diseases and autoimmunity. Umbilical cord mesenchymal stem cells (UCMSCs) have powerful regenerative and immunomodulatory potential, and their secreted extracellular vesicles (EVs) are envisaged as a promising natural source of nanoparticles to increase outcomes in organ transplantation and control inflammatory diseases. However, poor EV preparations containing highly-abundant soluble proteins may mask genuine vesicular-associated functions and provide misleading data...
2017: Theranostics
https://www.readbyqxmd.com/read/28004360/minimally-invasive-adipose-graft-transposition-procedure
#20
Carolina Gálvez-Montón, Paloma Gastelurrutia, Idoia Diaz-Güemes, Francisco M Sanchez-Margallo, Antoni Bayes-Genis
Recently, a new surgical technique to rescue infarcted myocardium (AGTP) has already tested (NCT01473433, AdiFLAP Trial). Here, we present the new minimally invasive AGTP (mi-AGTP) by thoracoscopy.
February 2017: Journal of Cardiovascular Translational Research
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