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Leydig cell toxicity

Mine Senyildiz, Buket Alpertunga, Sibel Ozden
3-Monochloropropane-1,2-diol (3-MCPD) is a well-known food processing contaminant that has been regarded as a rat carcinogen, which is known to induce Leydig-cell and mammary gland tumors in males, as well as kidney tumors in both genders. 3-MCPD is highly suspected to be a non-genotoxic carcinogen. 2,3-Epoxy-1-propanol (glycidol) can be formed via dehalogenation from 3-MCPD. We aimed to investigate the cytotoxic effects of 3-MCPD and glycidol, then to demonstrate the possible epigenetic mechanisms with global and gene-specific DNA methylation in rat kidney epithelial cells (NRK-52E)...
November 24, 2016: Drug and Chemical Toxicology
Jianxia Sun, Wei Xu, Cuijuan Zhu, Yunfeng Hu, Xinwei Jiang, Shiyi Ou, Zhijian Su, Yadong Huang, Rui Jiao, Weibin Bai
1,3-Dichloro-2-propanol (1,3-DCP) is a food processing contaminant and has been shown to perturb male reproductive function. Cyanidin-3-O-glucoside (C3G), an anthocyanin antioxidant, is reported to have protective effects on many organs. However, it remains unclear whether C3G protects against chemical-induced reproductive toxicity. The present study was therefore to investigate the intervention of C3G on 1,3-DCP-induced reproductive toxicity in R2C Leydig cells. Results demonstrated that C3G inhibited the 1,3-DCP-induced cytotoxicity and cell shape damage with the effective doses being ranging from 10 to 40 μmol/L...
2016: Frontiers in Pharmacology
Yong Fa Zhang, Jian Ying Yang, Yong Kai Li, Wei Zhou
To explore the toxic mechanism of T-2 toxin on Leydig cells of mice, we would investigate the toxicity and oxidative stress induced by T-2 toxin in the cells. Leydig cells were isolated and cultured with control or T-2 toxin (10(-7) M, 10(-8) M, or 10(-9) M) for 24 h, then cells and supernatants were harvested to examine cell viability, superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT) activities, expression of messenger RNA (mRNA) related to oxidative stress, malondialdehyde (MDA) content and DNA damage...
November 29, 2016: Toxicology Mechanisms and Methods
Zahra Noorimotlagh, Neemat Jaafarzadeh Haghighi, Mehdi Ahmadimoghadam, Fakher Rahim
Diverse industries like detergents, resins and polymers, hair dyes, intravaginal spermicides, and pesticides produce endocrine disruptor (ED)-containing wastewaters that have hazardous effects on the environment and public health. Nonylphenol (NP) is a chemical substance that consists of a phenolic group and an attached lipophilic linear nonyl chain. NP has weak estrogenic activity and affects estrogen receptor (ER), as well as induces male infertility via a negative impact on spermatogenesis and sperm quality...
November 8, 2016: Environmental Science and Pollution Research International
Zhe Han, Qi Yan, Wei Ge, Zhi-Guo Liu, Sangiliyandi Gurunathan, Massimo De Felici, Wei Shen, Xi-Feng Zhang
BACKGROUND: Nanoscience and nanotechnology are developing rapidly, and the applications of nanoparticles (NPs) have been found in several fields. At present, NPs are widely used in traditional consumer and industrial products, however, the properties and safety of NPs are still unclear and there are concerns about their potential environmental and health effects. The aim of the present study was to investigate the potential toxicity of ZnO NPs on testicular cells using both in vitro and in vivo systems in a mouse experimental model...
2016: International Journal of Nanomedicine
Xueting Wang, Jie Ding, Zou Xiang, Peipei Jiang, Jing Du, Xiaodong Han
Microcystin-LR (MC-LR), which generates strong reproductive toxicity, reduces serum testosterone level without directly damaging Leydig cells. The purpose of this study was to identify the target cells of MC-LR in the rat hypothalamic-pituitary axis and to investigate the underlying mechanisms. We found that the gonadotropin-releasing hormone (GnRH) neurons were the direct target of MC-LR. In vivo results showed that upon exposure to rats, cells around the third ventricle of hypothalamus underwent apoptosis...
October 20, 2016: Toxicon: Official Journal of the International Society on Toxinology
M Zubair, M Ahmad, M K Saleemi, S T Gul, N Ahmad, S Umar
The objective of this study was to investigate the toxic effects of arsenic on testicular measurements and histology of adult Teddy goats bucks and to examine whether these toxic effects are scavenged by vitamin E. Twelve adult Teddy goat bucks were divided randomly into three groups, A, B and C. Group A was kept as control, the B was given sodium arsenite 5 mg/kg BW per day, and group C was fed with vitamin E 200 mg/kg BW per day + arsenic 5 mg/kg BW per day. This treatment was continued for 84 days. Analysis of data revealed that the testicular measurements (scrotal circumference, width, length and weight) were significantly reduced in arsenic-treated animals, whereas there were ameliorating effects of vitamin E on these parameters...
September 29, 2016: Andrologia
Sean Harris, Sara Pacheco Shubin, Susanna Wegner, Kirk Van Ness, Foad Green, Sung Woo Hong, Elaine M Faustman
Our 3-dimensional testis co-culture system (3D-TCS) represents a promising model of male reproductive toxicity which captures sensitive processes of male reproductive development and contains the main testes cell types (germ, Leydig and Sertoli cells). Macrophages are another cell type important for testicular function and help to modulate immuno-endocrine processes during testes development. Chemicals such as phthalate esters (PE's) affect macrophage function and testosterone production in the testes in vivo...
October 2016: Toxicology in Vitro: An International Journal Published in Association with BIBRA
Gülfidan Coşkun, Hülya Özgür, Şaban Doran, Sait Polat
BACKGROUND/AIM: The aim of this study was to determine the antioxidative effect of curcumin on nicotine-induced mice testis. MATERIALS AND METHODS: Sixty Swiss albino male mice were divided into five groups, each containing 12 mice. The first group was used as a control. To induce toxicity in the second and third group, nicotine (0.4 mg/kg/day) was injected intraperitoneally into mice for 14 and 28 days, respectively. The mice in the fourth and fifth group were injected with nicotine (0...
2016: Turkish Journal of Medical Sciences
Enis Uluçam, Elvan Bakar
BACKGROUND/AIM: This study investigated the effect of proanthocyanidin (PA) against formaldehyde (FA)-induced lipid peroxidation damage and morphological changes in rat testes. MATERIALS AND METHODS: Twenty-one Wistar albino rats were randomized into 3 groups: control, FA, and FA + PA groups. Plasma and tissue malondialdehyde (MDA) and total sialic acid (TSA) levels were measured. Testes tissues were observed by light and electron microscopy. RESULTS: TSA (plasma and tissue) levels decreased and MDA (plasma) significantly increased (P < 0...
2016: Turkish Journal of Medical Sciences
Annie Boisvert, Steven Jones, Leeyah Issop, Hanno C Erythropel, Vassilios Papadopoulos, Martine Culty
Plasticizers are indispensable additives providing flexibility and malleability to plastics. Among them, several phthalates, including di (2-ethylhexyl) phthalate (DEHP), have emerged as endocrine disruptors, leading to their restriction in consumer products and creating a need for new, safer plasticizers. The goal of this project was to use in vitro functional screening tools to select novel non-toxic plasticizers suitable for further in vivo evaluation. A panel of novel compounds with satisfactory plasticizer properties and biodegradability were tested, along with several commercial plasticizers, such as diisononyl-cyclohexane-1,2-dicarboxylate (DINCH®)...
October 2016: Environmental Research
Robert R Maronpot, Abraham Nyska, Jennifer E Foreman, Yuval Ramot
The Fischer 344 (F344) rat was used by the National Toxicology Program (NTP) for over 5 decades for toxicity and carcinogenicity studies. However, in 2006, the NTP decided to switch to a different rat stock due largely to high background control incidences of Leydig cell tumors (LCTs) and mononuclear cell leukemia (MNCL), also known as large granular lymphocytic (LGL) leukemia. In the current review, we aim (1) to provide a summary of NTP bioassays with treatment-associated effects involving MNCL and LCTs in addition to male F344-specific tunica vaginalis mesothelioma (TVM); (2) to describe important pathobiological differences between these F344 rat tumor responses and similar target tissue-tumor response in humans; and (3) to present the NTP reasons for switching away from the F344 rat...
September 2016: Critical Reviews in Toxicology
Xiaomei Liu, Linlin Xu, Jingcao Shen, Jinglei Wang, Wenli Ruan, Mei Yu, Jiaxiang Chen
BACKGROUND: As a plasticizer, plastic softener, and flame-retardant, tri-ortho-cresyl phosphate (TOCP) is and has been widely used in industry and reported to have a toxic effect on the male reproductive system in animals besides neurotoxicity and immunotoxicity. We have reported that TOCP inhibits spermatogenesis and induces autophagy of rat spermatogonial stem cells, but it is still unknown whether TOCP induces autophagy of mouse Leydig cells and its potential mechanism. METHODS: Cell viability was observed by MTT assay...
2016: Reproductive Biology and Endocrinology: RB&E
Steven Jones, Annie Boisvert, Andrada Naghi, Françoise Hullin-Matsuda, Peter Greimel, Toshihide Kobayashi, Vassilios Papadopoulos, Martine Culty
Previous work in our laboratory demonstrated that in-utero exposure to a mixture of the phytoestrogen Genistein (GEN), and plasticizer DEHP, induces short- and long-term alterations in testicular gene and protein expression different from individual exposures. These studies identified fetal and adult Leydig cells as sensitive targets for low dose endocrine disruptor (ED) mixtures. To further investigate the direct effects and mechanisms of toxicity of GEN and DEHP, MA-10 mouse tumor Leydig cells were exposed in-vitro to varying concentrations of GEN and MEHP, the principal bioactive metabolite of DEHP...
April 29, 2016: Toxicology
Mei Ha, Xie Guan, Li Wei, Peng Li, Min Yang, Changjiang Liu
Di-(2-ethylhexyl) phthalate (DEHP) has reproductive toxicity and can affect male reproductive development. In order to clarify adverse effects of DEHP on testicular physiology and testosterone production, Sprague-Dawley (SD) rats were dosed daily with DEHP by gavage for 30days; TM3 cells (mouse Leydig cell line) were treated with DEHP for 24h after pretreatment with vitamin C or U0126. Results indicated that the hypothalamic-pituitary-testis (HPT) axis was disturbed and serum testosterone, LH and FSH levels were decreased following DEHP exposure...
September 1, 2016: Science of the Total Environment
Zheng-jun Huang, Hui-qing Que, Hua-yi Peng, Sui Lin, Shim-min Guo, Li-ping Qian
The arrenotokous toxicity of triptolide was evaluated, and the rate of sperm abnormality, the changes of the lipid peroxide, the enzyme activity and the hormone in male rats were observed. With the negative and positive control group, the healthy rats were respectively given by gavage triptolide suspension at the dose of 0.025, 0.05, 0.1 mg x kg(-1) for 30 days. Then the rats were killed for the measurement of the indicators in testis and serum, as well as the study on the sperm abnormality. The results showed that the positive control group had significant difference, compared with the negative control group...
December 2015: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
Xiao Jiang, Hong-Qiang Chen, Zhi-Hong Cui, Li Yin, Wen-Long Zhang, Wen-Bin Liu, Fei Han, Lin Ao, Jia Cao, Jin-Yi Liu
Study of the joint action of xenobiotics is important to fully explore their toxicity and complete risk analysis. In this study, we investigated the effects of low-dose and combined exposure of bisphenol A (BPA) and diethylstilbestrol (DES) on the reproductive system in adult male rats. The results showed that the sperm motility decreased in the BPA/DES and combined groups. Sperm deformity ratios and histological lesions of the testes were significantly higher and more significant, respectively, in the combined group compared with the single treated groups...
April 2016: Environmental Toxicology and Pharmacology
Ahmed Ibrahim, Magda M Ali, Nasser S Abou-Khalil, Marwa F Ali
BACKGROUND: For the last few years, researchers have been interested in developing a method for chemical sterilization which may be a better alternative to surgical castration. An ideal chemical sterilant would be one that effectively arrests spermatogenesis and androgenesis as well as libido with absence of toxic or other side effects. Calcium chloride in various solutions and concentrations has been tested in many animal species, but few studies have been evaluated it in equines as a chemical sterilant...
2016: BMC Veterinary Research
Balal Yousaf, Amina, Guijian Liu, Ruwei Wang, Abdul Qadir, Muhammad Ubaid Ali, Qudsia Kanwal, Bushra Munir, Asmatullah, Zaigham Abbas
The current study presents the bisphenol A exposure and the ameliorative effects of Adiantum capillus-veneris on testicular toxicity induced by bisphenol A. Adult male albino rats were divided into five groups of five animals each: A (control), B (vehicle control), C (toxic), D (protective), and E (ameliorative) were served distilled water, olive oil, bisphenol A (BPA) at 100 mg/kg body weight, A. capillus-veneris plant extract at 25 mg/kg body weight, and BPA + A. capillus-veneris, respectively. All of the doses were administered orally for 15 days, and the rats were then sacrificed...
June 2016: Environmental Science and Pollution Research International
Yukiko Harada, Naoki Tanaka, Motoki Ichikawa, Yuji Kamijo, Eiko Sugiyama, Frank J Gonzalez, Toshifumi Aoyama
It was reported that 2,4-dichlorophenoxyacetic acid (2,4-D), a commonly used herbicide and a possible endocrine disruptor, can disturb spermatogenesis, but the precise mechanism is not understood. Since 2,4-D is a weak peroxisome proliferator in hepatocytes and peroxisome proliferator-activated receptor α (PPARα) is also expressed in Leydig cells, this study aimed to investigate the link between PPARα and 2,4-D-mediated testicular dysfunction. 2,4-D (130 mg/kg/day) was administered to wild-type and Ppara-null mice for 2 weeks, and the alterations in testis and testosterone/cholesterol metabolism in Leydig cells were examined...
February 2, 2016: Archives of Toxicology
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