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https://www.readbyqxmd.com/read/28212420/transcriptome-using-illumina-sequencing-reveals-the-traits-of-spermatogenesis-and-developing-testes-in-eriocheir-sinensis
#1
Gen-Liang Li, Hui Qian
Chinese mitten crab (Eriocheir sinensis) has the spermatozoa with typical aflagellate, decondensed chromatin, cup-shaped nuclei, and radial arms. However, the mechanism of spermatogenesis during which the specific spermatozoa are generated in this species is yet unclear. Here, the transcriptome of developing testis in E. sinensis was analyzed using the ways of RNA-seq and bioinformatics analysis to identify candidate genes potentially involved in development of testis and spermatogenesis. The Illumina HiSeq2500 sequencing of three replicons of samples produced a total of 145...
2017: PloS One
https://www.readbyqxmd.com/read/28211534/in-vitro-differentiation-of-fertile-sperm-from-cryopreserved-spermatogonia-of-the-endangered-endemic-cyprinid-honmoroko-gnathopogon-caerulescens
#2
Shogo Higaki, Manami Shimada, Kazuaki Kawamoto, Takaaki Todo, Toshihiro Kawasaki, Ikuo Tooyama, Yasuhiro Fujioka, Noriyoshi Sakai, Tatsuyuki Takada
Many endemic fish species are threatened with extinction. Conservation strategies and the restoration of endemic fish after extinction must therefore be investigated. Although sperm cryopreservation is indispensable for the conservation of endangered fishes, the limited number of mature fish and limited availability (volume and period) of sperm from small endemic fish hinders the optimization and practical use of this material. In this report, we demonstrate the in vitro differentiation of fertile sperm from cryopreserved spermatogonia of juveniles of the endangered small cyprinid honmoroko (Gnathopogon caerulescens), which is endemic to Lake Biwa in Japan...
February 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28206990/a-familial-study-of-azoospermic-men-identifies-three-novel-causative-mutations-in-three-new-human-azoospermia-genes
#3
Moran Gershoni, Ron Hauser, Leah Yogev, Ofer Lehavi, Foad Azem, Haim Yavetz, Shmuel Pietrokovski, Sandra E Kleiman
PURPOSE: Up to 1% of all men experience azoospermia, a condition of complete absence of sperm in the semen. The mechanisms and genes involved in spermatogenesis are mainly studied in model organisms, and their relevance to humans is unclear because human genetic studies are very scarce. Our objective was to uncover novel human mutations and genes causing azoospermia due to testicular meiotic maturation arrest. METHODS: Affected and unaffected siblings from three families were subjected to whole-exome or whole-genome sequencing, followed by comprehensive bioinformatics analyses to identify mutations suspected to cause azoospermia...
February 16, 2017: Genetics in Medicine: Official Journal of the American College of Medical Genetics
https://www.readbyqxmd.com/read/28205594/genome-editing-reveals-dmrt1-as-an-essential-male-sex-determining-gene-in-chinese-tongue-sole-cynoglossus-semilaevis
#4
Zhongkai Cui, Yun Liu, Wenwen Wang, Qian Wang, Ning Zhang, Fan Lin, Na Wang, Changwei Shao, Zhongdian Dong, Yangzhen Li, Yingming Yang, Mengzhu Hu, Hailong Li, Fengtao Gao, Zhanfei Wei, Liang Meng, Yang Liu, Min Wei, Ying Zhu, Hua Guo, Christopher H K Cheng, Manfred Schartl, Songlin Chen
Chinese tongue sole is a marine fish with ZW sex determination. Genome sequencing suggested that the Z-linked dmrt1 is a putative male determination gene, but direct genetic evidence is still lacking. Here we show that TALEN of dmrt1 efficiently induced mutations of this gene. The ZZ dmrt1 mutant fish developed ovary-like testis, and the spermatogenesis was disrupted. The female-related genes foxl2 and cyp19a1a were significantly increased in the gonad of the ZZ dmrt1 mutant. Conversely, the male-related genes Sox9a and Amh were significantly decreased...
February 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28205525/testicular-activin-and-follistatin-levels-are-elevated-during-the-course-of-experimental-autoimmune-epididymo-orchitis-in-mice
#5
Nour Nicolas, Vera Michel, Sudhanshu Bhushan, Eva Wahle, Susan Hayward, Helen Ludlow, David M de Kretser, Kate L Loveland, Hans-Christian Schuppe, Andreas Meinhardt, Mark P Hedger, Monika Fijak
Experimental autoimmune epididymo-orchitis (EAEO) is a model of chronic inflammation, induced by immunisation with testicular antigens, which reproduces the pathology of some types of human infertility. Activins A and B regulate spermatogenesis and steroidogenesis, but are also pro-inflammatory, pro-fibrotic cytokines. Expression of the activins and their endogenous antagonists, inhibin and follistatin, was examined in murine EAEO. Adult untreated and adjuvant-treated control mice showed no pathology. All mice immunised with testis antigens developed EAEO by 50 days, characterised by loss of germ cells, immune cell infiltration and fibrosis in the testis, similar to biopsies from human inflamed testis...
February 13, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28205273/in-vitro-responses-to-known-in-vivo-genotoxic-agents-in-mouse-germ-cells
#6
Khaled Habas, Martin H Brinkworth, Diana Anderson
Genotoxic compounds have induced DNA damage in male germ cells and have been associated with adverse clinical outcomes including enhanced risks for maternal, paternal and offspring health. DNA strand breaks represent a great threat to the genomic integrity of germ cells. Such integrity is essential to maintain spermatogenesis and prevent reproduction failure. The Comet assay results revealed that the incubation of isolated germ cells with n-ethyl-n-nitrosourea (ENU), 6-mercaptopurine (6-MP) and methyl methanesulphonate (MMS) led to increase in length of Olive tail moment and % tail DNA when compared with the untreated control cells and these effects were concentration-dependent...
February 16, 2017: Environmental and Molecular Mutagenesis
https://www.readbyqxmd.com/read/28205220/comprehensive-genome-profiling-of-single-sperm-cells-by-multiple-annealing-and-looping-based-amplification-cycles-and-next-generation-sequencing-from-carriers-of-robertsonian-translocation
#7
Yanwei Sha, Yankun Sha, Zhiyong Ji, Lu Ding, Qing Zhang, Honggen Ouyang, Shaobin Lin, Xu Wang, Lin Shao, Chong Shi, Ping Li, Yueqiang Song
Robertsonian translocation (RT) is a common cause for male infertility, recurrent pregnancy loss, and birth defects. Studying meiotic recombination in RT-carrier patients helps decipher the mechanism and improve the clinical management of infertility and birth defects caused by RT. Here we present a new method to study spermatogenesis on a single-gamete basis from two RT carriers. By using a combined single-cell whole-genome amplification and sequencing protocol, we comprehensively profiled the chromosomal copy number of 88 single sperms from two RT-carrier patients...
March 2017: Annals of Human Genetics
https://www.readbyqxmd.com/read/28203733/kinesins-in-spermatogenesis%C3%A2
#8
Dan-Dan Ma, Da-Hui Wang, Wan-Xi Yang
No abstract text is available yet for this article.
February 1, 2017: Biology of Reproduction
https://www.readbyqxmd.com/read/28202061/in-vitro-generation-of-sertoli-like-and-haploid-spermatid-like-cells-from-human-umbilical-cord-perivascular-cells
#9
Ekaterina Shlush, Leila Maghen, Sonja Swanson, Shlomit Kenigsberg, Sergey Moskovtsev, Tanya Barretto, Andrée Gauthier-Fisher, Clifford L Librach
BACKGROUND: First trimester (FTM) and term human umbilical cord-derived perivascular cells (HUCPVCs), which are rich sources of mesenchymal stem cells (MSCs), can give rise to Sertoli cell (SC)-like as well as haploid germ cell (GC)-like cells in vitro using culture conditions that recapitulate the testicular niche. Gamete-like cells have been produced ex vivo using pluripotent stem cells as well as MSCs. However, the production of functional gametes from human stem cells has yet to be achieved...
February 15, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28196865/s-palmitoylation-of-junctional-adhesion-molecule-c-regulates-its-tight-junction-localization-and-cell-migration
#10
Pornpun Aramsangtienchai, Nicole A Spiegelman, Ji Cao, Hening Lin
Junctional Adhesion Molecule C (JAM-C) is an immunoglobulin superfamily protein expressed in epithelial cells, endothelial cells, and leukocytes. JAM-C has been implicated in leukocyte transendothelial migration, angiogenesis, cell-adhesion, cell polarity, spermatogenesis and metastasis. Here, we show that JAM-C undergoes S-palmitoylation on two juxtamembrane cysteine residues, Cys264 and Cys265. We have identified DHHC7 as a JAM-C palmitoylating enzyme by screening all known palmitoyltransferases (PATs or DHHCs)...
February 14, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28196764/nuclear-and-membrane-progestin-receptors-in-the-european-eel-characterization-and-expression-in-vivo-through-spermatogenesis
#11
Marina Morini, David S Peñaranda, María C Vílchez, Rasoul Nourizadeh-Lillabadi, Anne-Gaëlle Lafont, Sylvie Dufour, Juan F Asturiano, Finn-Arne Weltzien, Luz Pérez
Characterization of all the progestin receptor genes (PRs) found in the European eel has been performed. There were five membrane PRs (mPRs): mPRα (alpha), mPRAL1 (alpha-like1), mPRAL2 (alpha-like2), mPRγ (gamma), mPRδ (delta) and two nuclear PRs (nPRs or PGRs): pgr1 and pgr2. In silico studies showed that the C and E(F) domains of Pgr are well conserved among vertebrates whereas the A/B domain is not. Phylogeny and synteny analyses suggest that eel duplicated pgr (pgr1 and pgr2) originated from the teleost-specific third whole genome duplication (3R)...
February 11, 2017: Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology
https://www.readbyqxmd.com/read/28196692/shisa6-confers-resistance-to-differentiation-promoting-wnt-%C3%AE-catenin-signaling-in-mouse-spermatogenic-stem-cells
#12
Moe Tokue, Kanako Ikami, Seiya Mizuno, Chiyo Takagi, Asuka Miyagi, Ritsuko Takada, Chiyo Noda, Yu Kitadate, Kenshiro Hara, Hiroko Mizuguchi, Takuya Sato, Makoto Mark Taketo, Fumihiro Sugiyama, Takehiko Ogawa, Satoru Kobayashi, Naoto Ueno, Satoru Takahashi, Shinji Takada, Shosei Yoshida
In the seminiferous tubules of mouse testes, a population of glial cell line-derived neurotrophic factor family receptor alpha 1 (GFRα1)-positive spermatogonia harbors the stem cell functionality and supports continual spermatogenesis, likely independent of asymmetric division or definitive niche control. Here, we show that activation of Wnt/β-catenin signaling promotes spermatogonial differentiation and reduces the GFRα1(+) cell pool. We further discovered that SHISA6 is a cell-autonomous Wnt inhibitor that is expressed in a restricted subset of GFRα1(+) cells and confers resistance to the Wnt/β-catenin signaling...
February 7, 2017: Stem Cell Reports
https://www.readbyqxmd.com/read/28192519/arachidonic-acid-triggers-ca2-i-increases-in-rat-round-spermatids-by-a-likely-gpr-activation-erk-signalling-and-er-acidic-compartments-ca2-release
#13
Joaquin Paillamanque, Ana Sanchez-Tusie, Emerson M Carmona, Claudia L Treviño, Carolina Sandoval, Francisco Nualart, Nelson Osses, Juan G Reyes
Arachidonic acid (AA), a compound secreted by Sertoli cells (SC) in a FSH-dependent manner, is able to induce the release of Ca2+ from internal stores in round spermatids and pachytene spermatocytes. In this study, the possible site(s) of action of AA in round spermatids, the signalling pathways associated and the intracellular Ca2+ stores targeted by AA-induced signalling were pharmacologically characterized by measuring intracellular Ca2+ using fluorescent Ca2+ probes. Our results suggest that AA acts by interacting with a fatty acid G protein coupled receptor, initiating a G protein signalling cascade that may involve PLA2 and ERK activation, which in turn opens intracellular ryanodine-sensitive channels as well as NAADP-sensitive channels in acidic intracellular Ca2+ stores...
2017: PloS One
https://www.readbyqxmd.com/read/28192182/male-reproductive-toxicity-of-crvi-in-utero-exposure-to-crvi-at-the-critical-window-of-testis-differentiation-represses-the-expression-of-sertoli-cell-tight-junction-proteins-and-hormone-receptors-in-adult-f1-progeny-rats
#14
Kathiresh M Kumar, Mariajoseph Michael Aruldhas, Sheerin L Banu, Balaji Sadasivam, Ganapathy Vengatesh, Karthik M Ganesh, Shobana Navaneethabalakrishnan, Ajith Kumar Navin, Felicia Mary Michael, Sankar Venkatachalam, Jone A Stanley, Ilangovan Ramachandran, Sakhila K Banu, Mohammad Abdulkader Akbarsha
The effect of gestational exposure to CrVI (occupational/environmental pollutant and target to Sertoli cells(SC)) was tested in a rat model during the testicular differentiation from the bipotential gonad may interrupt spermatogenesis by disrupting SC tight junctions(TJ) and it's proteins and hormone receptors. Pregnant Wistar rats were exposed to 50/100/200ppm CrVI through drinking water during embryonic days 9-14. On Postnatal day 120, testes were subjected to ion exchange chromatographic analysis revealed increased level of CrIII in SCs and germ cells, serum and testicular interstitial fluid(TIF)...
February 9, 2017: Reproductive Toxicology
https://www.readbyqxmd.com/read/28191733/a-novel-dynamic-expression-of-vasa-in-male-germ-cells-during-spermatogenesis-in-the-chinese-soft-shell-turtle-pelidiscus-sinensis
#15
Wei Li, Piaoyi Zhang, Xuling Wu, Xinping Zhu, Hongyan Xu
vasa gene encodes a highly conserved DEAD-box RNA helicase, required for germ cell development across animal kingdom. Vasa mutations cause male infertility in mammals. It has been widely used as a biomarker for studying animal fertility or manipulating germ cells in organisms. However, in reptilians, the functions of vasa gene involved in germ cell differentiation are largely unclear; this hampers the development of biological techniques and the improvement of the productivity in these species. Here a vasa cDNA was isolated in Chinese soft-shell turtle and it predicts a protein of 691 amino acid residues, which is 72%, 69%, 58%, 59%, and 54-56% identical to its homolog from mouse, platypus, frog, chicken, and fish, respectively, and named as PsVasa...
February 13, 2017: Journal of Experimental Zoology. Part B, Molecular and Developmental Evolution
https://www.readbyqxmd.com/read/28190776/regulators-of-alternative-polyadenylation-operate-at-the-transition-from-mitosis-to-meiosis
#16
Lingjuan Shan, Chan Wu, Di Chen, Lei Hou, Xin Li, Lixia Wang, Xiao Chu, Yifeng Hou, Zhaohui Wang
In the sexually reproductive organisms, gametes are produced by meiosis following a limited mitotic amplification. However, the intrinsic program switching cells from mitotic to meiotic cycle is unclear. Alternative polyadenylation (APA) is a highly conserved means of gene regulation and is achieved by the RNA 3'-processing machinery to generate diverse 3'UTR profiles. In Drosophila spermatogenesis, we observed distinct profiles of transcriptome-wide 3'UTR between mitotic and meiotic cells. In mutant germ cells stuck in mitosis, 3'UTRs of hundreds of genes were consistently shifted...
January 27, 2017: Journal of Genetics and Genomics, Yi Chuan Xue Bao
https://www.readbyqxmd.com/read/28188343/mll5-kmt2e-structure-function-and-clinical-relevance
#17
REVIEW
Xiaoming Zhang, Wisna Novera, Yan Zhang, Lih-Wen Deng
The mixed lineage leukemia (MLL) family of genes, also known as the lysine N-methyltransferase 2 (KMT2) family, are homologous to the evolutionarily conserved trithorax group that plays critical roles in the regulation of homeotic gene (HOX) expression and embryonic development. MLL5, assigned as KMT2E on the basis of its SET domain homology, was initially categorized under MLL (KMT2) family together with other six SET methyltransferase domain proteins (KMT2A-2D and 2F-2G). However, emerging evidence suggests that MLL5 is distinct from the other MLL (KMT2) family members, and the protein it encodes appears to lack intrinsic histone methyltransferase (HMT) activity towards histone substrates...
February 10, 2017: Cellular and Molecular Life Sciences: CMLS
https://www.readbyqxmd.com/read/28187504/extreme-spermatogenesis-failure-andrological-phenotype-and-intracytoplasmic-sperm-injection-outcomes
#18
P Plouvier, A-L Barbotin, F Boitrelle, D Dewailly, V Mitchell, J-M Rigot, V Lefebvre-Khalil, G Robin
Patients with very low sperm count through direct sperm examination can exhibit extreme oligozoospermia or cryptozoospermia (after centrifugation). The management of these patients is a real challenge for both clinicians and biologists. In this retrospective and comparative cohort study, we compared the andrological phenotype of patients with extreme alterations of spermatogenesis and assessed whether the origin of spermatozoa (testicular or ejaculate) had any influence on intracytoplasmic sperm injection (ICSI) outcomes...
February 10, 2017: Andrology
https://www.readbyqxmd.com/read/28185954/wip1-directly-dephosphorylates-nlk-and-increases-wnt-activity-during-germ-cell-development
#19
Seung-Ju Cho, Bok-Sik Cha, Ok-Seon Kwon, Jisun Lim, Dong-Myung Shin, Dong Wook Han, Tohru Ishitani, Eek-Hoon Jho, Albert J Fornace, Hyuk-Jin Cha
Mice null for wild-type p53-induced phosphatase 1 (WIP1) display defects in testis development and spermatogenesis, resulting in reduced fertility. However, the molecular mechanism underlying these abnormalities in the testis remains uncharacterized. We report that the phosphatase activity of WIP1 increases Wnt activity through Nemo-like kinase (NLK). WIP1 directly interacted with NLK, which is highly homologous to p38 MAPK, a WIP1 substrate, and dephosphorylated its activation site. The WIP1-mediated inhibition of NLK activity markedly decreased the phosphorylation of lymphoid enhancer-binding factor 1 (LEF1), disrupting its interaction with β-catenin...
February 6, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28185602/impaired-hypothalamic-pituitary-testicular-axis-activity-spermatogenesis-and-sperm-function-promote-infertility-in-males-with-lead-poisoning
#20
Jason Gandhi, Rafael J Hernandez, Andrew Chen, Noel L Smith, Yefim R Sheynkin, Gargi Joshi, Sardar Ali Khan
Lead poisoning is a stealthy threat to human physiological systems as chronic exposure can remain asymptomatic for long periods of time before symptoms manifest. We presently review the biophysical mechanisms of lead poisoning that contribute to male infertility. Environmental and occupational exposure of lead may adversely affect the hypothalamic-pituitary-testicular axis, impairing the induction of spermatogenesis. Dysfunction at the reproductive axis, namely testosterone suppression, is most susceptible and irreversible during pubertal development...
February 10, 2017: Zygote: the Biology of Gametes and Early Embryos
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