keyword
MENU ▼
Read by QxMD icon Read
search

Spermatogenesis

keyword
https://www.readbyqxmd.com/read/29232857/mir-34a-regulates-sperm-motility-in-zebrafish
#1
Wenjie Guo, Binyue Xie, Shuting Xiong, Xufang Liang, Jian-Fang Gui, Jie Mei
Increasing attention has been focused on the role of microRNAs in post-transcription regulation during spermatogenesis. Recently, the miR-34 family has been shown to be involved in the spermatogenesis, but the clear function of the miR-34 family in spermatogenesis is still obscure. Here we analyzed the function of miR-34a, a member of the miR-34 family, during spermatogenesis using miR-34a knockout zebrafish generated by the clustered regularly interspaced short palindromic repeats/associated protein 9 (CRISPR/Cas9) system...
December 10, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29230838/carnosine-prevents-testicular-oxidative-stress-and-advanced-glycation-end-product-formation-in-d-galactose-induced-aged-rats
#2
A F Aydın, C Küçükgergin, J Çoban, I Doğan-Ekici, S Doğru-Abbasoğlu, M Uysal, N Koçak-Toker
D-Galactose is shown to mimic natural ageing in rodents by exacerbating oxidative stress and glycation. Steroid production and having a poor antioxidant system make testis vulnerable to galactose-induced ageing. Antioxidation and antiglycating actions of carnosine may be intriguing for prevention of testicular ageing. In this study, male Wistar rats were applied D-galactose (300 mg/kg; subcutaneously 5 days a week) and carnosine (250 mg/kg; intraperitoneally 5 days a week) along with D-galactose for 2 months...
December 12, 2017: Andrologia
https://www.readbyqxmd.com/read/29229236/ultrastructural-analysis-of-early-toxic-effects-produced-by-bee-venom-phospholipase-a2-and-melittin-in-sertoli-cells-in-rats
#3
Mariana Tilinca, Adrian Florea
In this study, we aimed to investigate the testicular toxicity of two molecules derived from bee venom (BV): phospholipase A2 (PlA2) and melittin (Mlt). Ultrastructural effects of purified BV PlA2 and Mlt were assessed consecutive to repeated dose (30 days) and acute toxicity studies. For the subchronic treatment, PlA2 and Mlt were injected in daily doses equivalent to those released by a bee sting (105 μg PlA2/kg/day and 350 μg Mlt/kg/day), while in the acute treatment their doses corresponded to those released by 100 bee stings (9...
December 8, 2017: Toxicon: Official Journal of the International Society on Toxinology
https://www.readbyqxmd.com/read/29228727/prohibitin-mediated-mitochondrial-ubiquitination-during-spermiogenesis-in-chinese-mitten-crab-eriocheir-sinensis
#4
Cong-Cong Hou, Chao-Guang Wei, Cheng-Peng Lu, Xin-Ming Gao, Wan-Xi Yang, Jun-Quan Zhu
The sperm of Eriocheir sinensis has a cup-shaped nucleus that contains several mitochondria embedded at the opening of the cup. The acrosome vesicle also contains derivants of mitochondria. The mitochondria distribution pattern involves a decrease in the number and changes in the structure and transportation of these organelles. The decreased number of sperm mitochondria is achieved through autophagy or the ubiquitination pathway. Prohibitin (PHB), the mitochondria inner membrane protein, is an evolutionarily highly conserved protein, is closely associated with spermatogenesis and sperm quality control and is also a potential substrate of ubiquitination...
November 17, 2017: Oncotarget
https://www.readbyqxmd.com/read/29228719/levels-of-leydig-cell-autophagy-regulate-the-fertility-of-male-naked-mole-rats
#5
Wenjing Yang, Li Li, Xiaofeng Huang, Guanghan Kan, Lifang Lin, Jishuai Cheng, Chen Xu, Wei Sun, Wei Cong, Shanmin Zhao, Shufang Cui
Fertility is abolished in nonbreeding males in colonies of natal naked mole-rats (NMRs). Although spermatogenesis occurs in both breeding and nonbreeding male NMRs, the mechanisms underlying the differences in fertility between breeders and nonbreeders remain unexplored. In this study, a significant decrease in autophagy was observed in Leydig cells of the testis from nonbreeding male NMRs. This alteration was visualised as a significant decrease in the levels of autophagy-related gene 7 (Atg7), Atg5, microtubule-associated protein 1A/B light chain 3 (LC3-II/I) and the number of autophagosomes and an increase in P62 levels using Western blotting analyses...
November 17, 2017: Oncotarget
https://www.readbyqxmd.com/read/29228103/fertility-impairment-with-defective-spermatogenesis-and-steroidogenesis-in-male-zebrafish-lacking-androgen-receptor
#6
Haipei Tang, Yu Chen, Le Wang, Yike Yin, Gaofei Li, Yin Guo, Yun Liu, Haoran Lin, Christopher H K Cheng, Xiaochun Liu
The pivotal role of androgen receptor (AR) in regulating male fertility has attracted much research attention in the past two decades. Previous studies have shown that total androgen receptor knockout would lead to incomplete spermatogenesis and a lowered serum testosterone levels in mice, resulting in azoospermia and infertility. However, the precise physiological role of ar in controlling fertility of male fish is still poorly understood. In this study, we have established an ar knockout zebrafish line by transcription activator-like effectors nucleases (TALENs)...
December 6, 2017: Biology of Reproduction
https://www.readbyqxmd.com/read/29222434/embryonic-lethality-and-defective-male-germ-cell-development-in-mice-lacking-utf1
#7
Seth D Kasowitz, Mengcheng Luo, Jun Ma, N Adrian Leu, P Jeremy Wang
The germ cell lineage is specified early in embryogenesis and undergoes complex developmental programs to generate gametes. Here, we conducted genetic studies to investigate the role of Utf1 (Undifferentiated embryonic cell transcription factor 1) in mouse germ cell development. Utf1 is expressed in pluripotent embryonic stem (ES) cells and regulates ES cell differentiation. In a proteomics screen, we identified UTF1 among 38 proteins including DNMT3L and DND1 that associate with chromatin in embryonic testes...
December 8, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29216724/inhibition-by-egb761-of-the-effect-of-cellphone-radiation-on-the-male-reproductive-system
#8
F Gevrek, D Aydin, S Ozsoy, H Aygun, C Bicer
OBJECTIVES: To determine the effects of Egb761 on testicular tissues and semen parameters in rats exposed to cellphone waves. BACKGROUND: EGb761 has antioxidant properties as a free-radical scavenger. Cellphone electromagnetic radiation (EMR) induces oxidative stress in cells. METHODS: Twenty-one Wistar albino male adult rats were divided into three groups (control, experimental, treatment), including seven rats in each. The experimental and treatment groups were exposed to cellphone EMR (0...
2017: Bratislavské Lekárske Listy
https://www.readbyqxmd.com/read/29209278/follicle-stimulating-hormone-regulates-igfbp-gene-expression-directly-or-via-downstream-effectors-to-modulate-igf3-effects-on-zebrafish-spermatogenesis
#9
Diego Safian, Henk J G van der Kant, Diego Crespo, Jan Bogerd, Rüdiger W Schulz
Previous work showed that pharmacological inactivation of Igf-binding proteins (Igfbps), modulators of Igf activity, resulted in an excessive differentiation of type A undifferentiated (Aund) spermatogonia in zebrafish testis in tissue culture when Fsh was present in the incubation medium. Using this testis tissue culture system, we studied here the regulation of igfbp transcript levels by Fsh and two of its downstream effectors, Igf3 and 11-ketotestosterone (11-KT). We also explored how Fsh-modulated igfbp expression affected spermatogonial proliferation by adding or removing the Igfbp inhibitor NBI-31772 at different times...
2017: Frontiers in Endocrinology
https://www.readbyqxmd.com/read/29209210/critical-role-of-hepatic-cyp450s-in-the-testis-specific-toxicity-of-5r-5-hydroxytriptolide-in-c57bl-6-mice
#10
Cunzhi Yu, Yu Li, Mingxia Liu, Man Gao, Chenggang Li, Hong Yan, Chunzhu Li, Lihan Sun, Liying Mo, Chunyong Wu, Xinming Qi, Jin Ren
Low solubility, tissue accumulation, and toxicity are chief obstacles to developing triptolide derivatives, so a better understanding of the pharmacokinetics and toxicity of triptolide derivatives will help with these limitations. To address this, we studied pharmacokinetics and toxicity of (5R)-5-hydroxytriptolide (LLDT-8), a novel triptolide derivative immunosuppressant in a conditional knockout (KO) mouse model with liver-specific deletion of CYP450 reductase. Compared to wild type (WT) mice, after LLDT-8 treatment, KO mice suffered severe testicular toxicity (decreased testicular weight, spermatocytes apoptosis) unlike WT mice...
2017: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/29208639/reconstitution-of-female-germ-cell-fate-determination-and-meiotic-initiation-in-mammals
#11
So I Nagaoka, Mitinori Saitou
Meiosis is a fundamental process that underpins sexual reproduction. In mammals, the execution of meiosis is tightly integrated within the complex processes of oogenesis and spermatogenesis, and elucidation of the molecular mechanisms regulating meiotic initiation remains challenging. We have recently developed in vitro culture strategies to induce mouse pluripotent stem cells into germ cells, which successfully contribute to both oogenesis and spermatogenesis and to fertile offspring. The culture strategies faithfully recapitulate transcriptional and epigenetic dynamics as well as signaling principles for germ cell specification, proliferation, and female sex determination/meiotic induction, providing a valuable platform for studies to illuminate the molecular mechanisms underlying such critical processes...
December 5, 2017: Cold Spring Harbor Symposia on Quantitative Biology
https://www.readbyqxmd.com/read/29207191/novel-evidence-that-pituitary-sex-hormones-regulate-migration-adhesion-and-proliferation-of-embryonic-stem-cells-and-teratocarcinoma-cells
#12
Zachariah Payne Sellers, Kamila Bujko, Gabriela Schneider, Magdalena Kucia, Mariusz Z Ratajczak
The pituitary sex hormones (SexHs): follicle‑stimulating hormone (FSH), luteinizing hormone (LH), and prolactin (PRL) regulate several functions crucial for reproduction, including oogenesis, spermatogenesis, and lactation. An important source of prolactin-like hormones, known as lactogens, is the placenta, and lactogens bind to the PRL receptor (PRLR) with high affinity and thereby mimic the actions of PRL. Recently, it has been demonstrated that pituitary SexHs were involved in metastatic lung cancer, certain sarcomas, and leukemia...
November 24, 2017: Oncology Reports
https://www.readbyqxmd.com/read/29207172/ran-binding-protein-m-is-associated-with-human-spermatogenesis-and-oogenesis
#13
Wen-Hao Tang, Xin-Jie Zhuang, Shi-De Song, Han Wu, Zhe Zhang, Yu-Zhuo Yang, Hong-Liang Zhang, Jia-Ming Mao, De-Feng Liu, Lian-Ming Zhao, Hao-Cheng Lin, Kai Hong, Lu-Lin Ma, Jie Qiao, Weibing Qin, Yunge Tang, Hui Jiang
The aim of the present study was to explore the underlying mechanism and diagnostic potential of Ran‑binding protein M (RanBPM) in human spermatogenesis and oogenesis. RanBPM expression in human testis and ovaries was analysed using polymerase chain reaction (PCR) and western blotting, and immunofluorescence was performed on testis and ovary tissue sections during different developmental stages of spermatogenesis and oogenesis using RanBPM antibodies. Interactions with a variety of functional proteins were also investigated...
November 23, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29203529/crystal-and-solution-structures-of-human-protein-disulfide-isomerase-like-protein-of-the-testis-pdilt-provide-insight-into-its-chaperone-activity
#14
Huanhuan Li, Kai Yang, Wenjia Wang, Yingbo Niu, Jun Li, Yuhui Dong, Yingfang Liu, Chih-Chen Wang, Lei Wang, Huanhuan Liang
Protein disulfide isomerase-like protein of the testis (PDILT), a member of the protein disulfide isomerase (PDI) family is a chaperone essential for the folding of spermatogenesis-specific proteins in male postmeiotic germ cells. However, the structural mechanisms that regulate the chaperone function of PDILT are unknown. Here, we report the structures of human PDILT (hPDILT) determined by X-ray crystallography to 2.4 angstrom resolution and small-angle X-ray scattering (SAXS). Distinct from previously reported U-like structures of related PDI family proteins, our structures revealed that hPDILT folds into a compact L-like structure in crystals and into an extended chain-like structure in solution...
December 4, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29202267/decrease-in-male-mouse-fertility-by-hydrogen-sulfide-and-or-ammonia-can-be-inheritable
#15
Weidong Zhang, Yong Zhao, Pengfei Zhang, Yanan Hao, Shuai Yu, Lingjiang Min, Lan Li, Dongxue Ma, Liang Chen, Bao Yi, Xiangfang Tang, Qingshi Meng, Lei Liu, Shukun Wang, Wei Shen, Hongfu Zhang
Numerous epidemiological studies suggest that air pollutants cause a decline in the quality of human spermatozoa and thus a reduction in fertility. However, the exact cause of infertility remains unknown. Air pollution gases, such as NH3 and H2S are either free or bound to airborne particular materials (PM) and are abundant and reactive. The aim of this current investigation was to explore the impacts of NH3 and/or H2S on male fertility and the underlying mechanisms. Male mouse exposed to H2S and/or NH3 and after two generations were used to evaluate the impacts on fertility...
November 30, 2017: Chemosphere
https://www.readbyqxmd.com/read/29201907/developmental-testicular-expression-cloning-and-characterization-of-rat-hdac6-in-silico
#16
Pratibha Verma, Omshree Shetty, Sweta Parab, Karen Menezes, Priyanka Parte
We had previously reported presence of histone deacetylase 6 (HDAC6) in sperm and demonstrated its tubulin deacetylase activity and role in sperm motility in rat. In the present study we report its abundant expression in testis, epididymis, accessory sex organs, brain, and adrenal. In the testis, HDAC6 transcript and protein were observed throughout development. We therefore cloned the gene from rat testis using primers for hdac6 (accession number XM_228753.8) in order to determine the role of acetylation/deacetylation in spermatogenesis...
2017: BioMed Research International
https://www.readbyqxmd.com/read/29198592/can-tangier-disease-cause-male-infertility-a-case-report-and-an-overview-on-genetic-causes-of-male-infertility-and-hormonal-axis-involved
#17
Laura Stocchi, Emiliano Giardina, Luigia Varriale, Annalisa Sechi, Andrea Vagnini, Gianni Parri, Massimo Valentini, Maria Capalbo
Tangier disease is an autosomal recessive disorder caused by mutations in the ABCA1 gene and characterized by the accumulation of cholesteryl ester in various tissues and a near absence of high-density lipoprotein. The subject in this investigation was a 36-year-old Italian man with Tangier disease. He and his wife had come to the In Vitro Fertilization Unit, Pesaro Hospital (Azienda Ospedaliera Ospedali Riuniti Marche Nord) seeking help regarding fertility issues. The man was diagnosed with severe oligoasthenoteratozoospermia...
November 26, 2017: Molecular Genetics and Metabolism
https://www.readbyqxmd.com/read/29198215/dietary-l-arginine-supplementation-improves-semen-quality-and-libido-of-boars-under-high-ambient-temperature
#18
J Q Chen, Y S Li, Z J Li, H X Lu, P Q Zhu, C M Li
l-Arginine is a nutritionally essential amino acid for spermatogenesis and plays versatile roles in animal health and can be utilized as a potential agent to improve reproductive performance of boars under high ambient temperature. The present study aimed to determine whether dietary l-arginine could alleviate heat stress-induced infertility in boars. In all, 20 boars (PIC 1040; 248.59±3.84 kg BW and 407.65±6.40 days of age) were selected and randomly assigned to four groups (group 0.0%, basal diet; group 0...
December 4, 2017: Animal: An International Journal of Animal Bioscience
https://www.readbyqxmd.com/read/29197623/dual-effects-of-sulfasalazine-on-rat-sperm-characteristics-spermatogenesis-and-steroidogenesis-in-two-experimental-models
#19
Mohammad Mehdi Ommati, Reza Heidari, Akram Jamshidzadeh, Mohammad Javad Zamiri, Zilong Sun, Samira Sabouri, Jundong Wang, Fatemeh Ahmadi, Nafiseh Javanmard, Kazem Seifi, Saeed Mousapour, Babak Shirazi Yeganeh
There are reports of sulfasalazine (Salazosulfapyridine; SASP)-induced reproductive toxicity, but there it is not known whether the SASP molecule or its intestinal metabolites are responsible for this effect. Rats received SASP (150, 300, and 600mg/kg) for 60 consecutive days (in vivo). Additionally, epididymal sperm was isolated and incubated with SASP (10μM-1600μM) (in vitro). Markers of oxidative stress, mitochondrial function, and sperm functionality, along with testis histopathology as well as several steroidogenic genes and proteins, including steroidogenic acute regulatory (StAR) protein, cytochrome P450 side chain cleavage enzyme (P450scc; Cyp11a), 3β-hydroxysteroid dehydrogenase (3β-HSD), 17β-hydroxysteroid dehydrogenase (17β-HSD) were measured...
November 29, 2017: Toxicology Letters
https://www.readbyqxmd.com/read/29197587/analysis-of-mirna-expression-profiles-in-melatonin-exposed-gc-1-spg-cell-line
#20
Xiaoling Zhu, Shuxiong Chen, Yanwen Jiang, Ying Xu, Yun Zhao, Lu Chen, Chunjin Li, Xu Zhou
Melatonin is an endocrine neurohormone secreted by pinealocytes in the pineal gland. It exerts diverse physiological effects, such as circadian rhythm regulator and antioxidant. However, the functional importance of melatonin in spermatogenesis regulation remains unclear. The objectives of this study are to: (1) detect melatonin affection on miRNA expression profiles in GC-1 spg cells by miRNA deep sequencing (DeepSeq) and (2) define melatonin affected miRNA-mRNA interactions and associated biological processes using bioinformatics analysis...
November 29, 2017: Gene
keyword
keyword
28876
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"