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https://www.readbyqxmd.com/read/28087634/genome-wide-identification-of-regulatory-elements-in-sertoli-cells
#1
Danielle M Maatouk, Anirudh Natarajan, Yoichiro Shibata, Lingyun Song, Gregory E Crawford, Uwe Ohler, Blanche Capel
A current goal of molecular biology is to identify transcriptional networks regulating cell differentiation. However, identifying functional gene regulatory elements has been challenging in the context of developing tissues where material is limited and cell types are mixed. To identify regulatory sites during sex determination, we subjected Sertoli cells from mouse fetal testes to DNaseI-seq and ChIP-seq for H3K27ac. DNaseI-seq identified putative regulatory sites around Sertoli- and pregranulosa-enriched genes; however, active enhancers marked by H3K27ac were enriched proximal only to Sertoli-enriched genes...
January 13, 2017: Development
https://www.readbyqxmd.com/read/28065881/an-integrated-transcriptomics-guided-genome-wide-promoter-analysis-and-next-generation-proteomics-approach-to-mine-factor-s-regulating-cellular-differentiation
#2
Kamal Mandal, Samuel L Bader, Pankaj Kumar, Dipankar Malakar, David S Campbell, Bhola Shankar Pradhan, Rajesh K Sarkar, Neerja Wadhwa, Souvik Sensharma, Vaibhav Jain, Robert L Moritz, Subeer S Majumdar
Differential next-generation-omics approaches aid in the visualization of biological processes and pave the way for divulging important events and/or interactions leading to a functional output at cellular or systems level. To this end, we undertook an integrated Nextgen transcriptomics and proteomics approach to divulge differential gene expression of infant and pubertal rat Sertoli cells (Sc).Unlike, pubertal Sc, infant Sc are immature and fail to support spermatogenesis. We found exclusive association of 14 and 19 transcription factor binding sites to infantile and pubertal states of Sc, respectively, using differential transcriptomics-guided genome-wide computational analysis of relevant promoters employing 220 Positional Weight Matrices from the TRANSFAC database...
January 8, 2017: DNA Research: An International Journal for Rapid Publication of Reports on Genes and Genomes
https://www.readbyqxmd.com/read/28061524/prevalence-of-small-testicular-hyperechogenic-foci-in-subgroups-of-382-non-vasectomized-azoospermic-men-a-retrospective-cohort-study
#3
J Fedder
Testicular hyperechogenic foci (THF) are associated with Klinefelter's syndrome, cryptorchidism, infertility, and testicular germ cell neoplasia. The aims of the study were to evaluate THF in relation to etiology of azoospermia and to Sertoli cell dysfunction. The structures inside the scrotum of consecutive non-vasectomized, azoospermic were examined by ultrasonography, and hormone (FSH, LH, testosterone, and prolactin), and genetic analyses (karyotype, Y microdeletions, and CFTR mutations) were performed...
January 6, 2017: Andrology
https://www.readbyqxmd.com/read/28057115/-effects-of-extracellular-regulated-protein-kinases-protein-and-impairment-of-blood-testis-barriar-stucturein-of-mice-with-exposure-to-decabromodiphenyl-ether
#4
J Li, R X Dai, D J Chen, C M Wang, H F Lin, Y R Li, J Tang, J X Zhai
Objective: To study changes in expression of claudin-11 and proteins related to mitogen-activated protein kinase (MAPK) signaling pathways, as well as the ultrastructure of the blood testis barrier (BTB), in male ICR mice exposed to decabromodiphenyl ether (BDE-209). Methods: Fifty-two mice, 4 weeks of age, weighing 15-21 g, were provided with adaptive feeding for 1 week. Mice were randomly divided into 4 groups, named control, low-dose, medium-dose and high-dose groups. The treated groups received BDE-209, by intragastric gavage, at doses, respectively, of 100, 300 and 500 mg/kg...
December 6, 2016: Zhonghua Yu Fang Yi Xue za Zhi [Chinese Journal of Preventive Medicine]
https://www.readbyqxmd.com/read/28053620/establishment-of-a-surgically-induced-cryptorchidism-canine-recipient-model-for-spermatogonial-stem-cell-transplantation
#5
Won-Young Lee, Ran Lee, Hyuk Song, Tai-Young Hur, Seunghoon Lee, Jiyun Ahn, Hyunjhung Jhun
Transplantation of spermatogonial stem cells (SSCs) in experimental animal models has been used to study germ line stem cell biology and to produce transgenic animals. The species-specific recipient model preparation is important for the characterization of SSCs and the production of offspring. Here, we investigated the effects of surgically induced cryptorchidism in dog as a new recipient model for spermatogonial stem cell transplantation. Artificially unilateral or bilateral cryptorchidism was induced in ten mature male dogs by surgically returning the testis and epididymis to the abdominal cavity...
December 2016: Laboratory Animal Research
https://www.readbyqxmd.com/read/28051360/the-notch-ligand-jag1-regulates-gdnf-expression-in-sertoli-cells
#6
Thomas Xavier Garcia, Parag Parekh, Pooja Gandhi, Krishna M Sinha, Marie-Claude Hofmann
In the seminiferous epithelium of the testis, Sertoli cells are key niche cells directing proliferation and differentiation of spermatogonial stem cells (SSCs) into spermatozoa. Sertoli cells produce glial cell line-derived neurotrophic factor (GDNF), which is essential for SSC self-renewal and progenitor expansion. While the role of GDNF in the testis stem cell niche is established, little is known about how this factor is regulated. Our previous studies on NOTCH activity in Sertoli cells demonstrated a role of this pathway in limiting stem/progenitor cell numbers, thus ultimately down-regulating sperm cell output...
January 4, 2017: Stem Cells and Development
https://www.readbyqxmd.com/read/28050378/large-cell-calcifying-sertoli-cell-tumour-of-testis-a-rare-case-report
#7
Harresh Kumar, Nadeem Tanveer, Natasha Gupta, Kiran Mishra
Sertoli cell tumours of testes are classified into sertoli cell tumour NOS (not otherwise specified), sclerosing variant and large cell calcifying variant. So far, 90 cases of the large cell calcifying variant have been reported in literature. We describe a rare case of inhibin negative locally invasive large cell calcifying sertoli cell tumour of testis. A 62-year-old man presented with complaints of pain and swelling in right scrotum for 8 months. Ultrasound revealed a right testicular mass with internal vascularity and calcification...
November 2016: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28045957/normal-levels-of-sox9-expression-in-the-developing-mouse-testis-depend-on-the-tes-tesco-enhancer-but-this-does-not-act-alone
#8
Nitzan Gonen, Alexander Quinn, Helen C O'Neill, Peter Koopman, Robin Lovell-Badge
During mouse sex determination, transient expression of the Y-linked gene Sry up-regulates its direct target gene Sox9, via a 3.2 kb testis specific enhancer of Sox9 (TES), which includes a core 1.4 kb element, TESCO. SOX9 activity leads to differentiation of Sertoli cells, rather than granulosa cells from the bipotential supporting cell precursor lineage. Here, we present functional analysis of TES/TESCO, using CRISPR/Cas9 genome editing in mice. Deletion of TESCO or TES reduced Sox9 expression levels in XY fetal gonads to 60 or 45% respectively relative to wild type gonads, and reduced expression of the SOX9 target Amh...
January 2017: PLoS Genetics
https://www.readbyqxmd.com/read/28045098/effects-of-elevated-%C3%AE-estradiol-levels-on-the-functional-morphology-of-the-testis-new-insights
#9
Myles Leavy, Matthias Trottmann, Bernhard Liedl, Sven Reese, Christian Stief, Benjamin Freitag, John Baugh, Giulio Spagnoli, Sabine Kölle
Elevated estradiol levels are correlated with male infertility. Causes of hyperestrogenism include diseases of the adrenal cortex, testis or medications affecting the hypothalamus-pituitary-gonadal axis. The aim of our study was to elucidate the effects of estradiol treatment on testicular cellular morphology and function, with reference to the treatment regimen received. Testes samples (n = 9) were obtained post-orchiectomy from male-to-female transsexuals within the age range of 26-52 years. Each patient had a minimum of 1-6 years estradiol treatment...
January 3, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28043349/effects-of-guanidinoacetic-acid-diet-supplementation-on-semen-quality-and-fertility-of-broiler-breeder-roosters
#10
Ramin Shahabi Tapeh, Mahdi Zhandi, Mojtaba Zaghari, Amir Akhlaghi
Decreased semen quality and fertility rate is a common feature in broiler breeder roosters. This decrease is associated with dysfunction of Sertoli cells and defective spermatogenesis. Guanidinoacetic acid (GAA), as a precursor of creatine, plays an important role in the proper functioning of Sertoli cells and energy metabolism in sperm. Twenty, 29-wk-old broiler breeder roosters (Ross 308) were randomly allotted to 4 treatment groups and fed diets supplemented with different levels of GAA, including 0 (GAA-0), 600 (GAA-600), 1200 (GAA-1200), and 1800 (GAA-1800) mg GAA/kg of diet for 26 successive weeks...
February 2017: Theriogenology
https://www.readbyqxmd.com/read/28043339/age-related-changes-in-transcriptional-abundance-and-circulating-levels-of-anti-mullerian-hormone-and-sertoli-cell-count-in-crossbred-and-zebu-bovine-males
#11
S K Rajak, A Kumaresan, N M Attupuram, S Chhillar, R K Baithalu, S Nayak, L Sreela, Raushan K Singh, U K Tripathi, T K Mohanty, Savita Yadav
Age-related changes in peripheral anti-Mullerian hormone (AMH) concentrations and transcriptional abundance of AMH gene in testicular tissue were studied in crossbred (Holstein Friesian × Tharparkar) and Zebu (Tharparkar) males. In both the breeds, basal AMH concentrations were estimated using ELISA method in blood plasma obtained from six males each at 1, 6, 12, 18, and 24 months age. After blood collection at respective ages, all the males were castrated and expression and immunolocalization of AMH was performed in the testicular tissue...
February 2017: Theriogenology
https://www.readbyqxmd.com/read/28043263/-effect-of-fluorochloridone-on-oxidative-stress-in-primary-co-cultured-sertoli-germ-cell-of-rat
#12
S L Wu, L R Xu, Z J Zhou
Objective: To explore effect of fluorochloridone on primary co-cultured sertoli-germ cell of rat and its possible mechanism. Methods: primary co-cultured sertoli-germ cell was made by two steps of en-zyme digestion with SD rat testes, after 24h of Sertoli-germ cell isolation, A 0.1% DMSO solvent control group and three FLC exposure groups (10(-6)、10(-7)、10(-8) mol/L) were selected, cultured cell for 24h, then MTT assay and index detection of oxidative stress were performed. Results: The mortality of primary co-cultured sertoli-germ cell exposed to 10(-6) mol/L FLC was significantly higher than control group and FLC exposure groups (10(-7)、10(-8) mol/L) (P<0...
November 20, 2016: Chinese Journal of Industrial Hygiene and Occupational Diseases
https://www.readbyqxmd.com/read/28041982/4-nonylphenol-induces-autophagy-and-attenuates-mtor-p70s6k-4ebp1-signaling-by-modulating-ampk-activation-in-sertoli-cells
#13
Peng Duan, Chunhui Hu, Chao Quan, Tingting Yu, Wenting Huang, Wei Chen, Sha Tang, Yuqin Shi, Francis L Martin, Kedi Yang
The estrogenic chemical 4-nonylphenol (NP) is known to impair testicular devolopment and spermatogenesis in rodents. The objective of this study was to explore the effects of NP on autophagy induction and AMPK-mTOR signaling pathway in Sertoli cells (SCs), which are the "nursemaid cells" for meiosis of spermatocytes. In this study we exposed 7-week-old male rats to NP by intra-peritoneal injection at 0, 20, 50 or 100mg/kg body weight/2days for 20 consecutive days. Our results showed that exposure to NP dose-dependently induces the formation of autophagosomes in SCs, increases the expression of Beclin-1, the conversion of LC3-I to LC3-II and the mRNA expression of Atg3, Atg5, Atg7 and Atg12 in testis, and these effects are concomitant with the activation of AMPK, and the suppression of TSC2-mTOR-p70S6K/4EBP1 signaling cascade in testis...
December 29, 2016: Toxicology Letters
https://www.readbyqxmd.com/read/28039344/complete-androgen-insensitivity-syndrome-with-concomitant-seminoma-and-sertoli-cell-adenoma-an-unusual-combination
#14
Balamurugan Thirunavukkarasu, Asit Ranjan Mridha, Neena Malhotra, Sheragaru Hanumanthappa Chandrashekhara
Androgen insensitivity syndrome is a rare disorder of sex development and its clinical manifestations vary from subtle male infertility to an overt complete androgen insensitivity syndrome (CAIS) with a female phenotype. CAIS is often diagnosed at puberty or in adolescence during investigation for primary amenorrhoea. Undiagnosed patients have an increased risk of development of malignancy in the harboured testes. Inguinal hernia is the commonest mode of presentation of CAIS in childhood and various screening methods are available during the initial herniorrhaphy procedure...
December 30, 2016: BMJ Case Reports
https://www.readbyqxmd.com/read/28018460/unusual-malignant-neoplasms-of-ovary-in-children-two-cases-report
#15
Ali Ghribi, Aicha Bouden, Manef Gasmi, Mourad Hamzaoui
Sex cord tumors with annular tubules are known to originate from the sex cord of embryonic gonads that synthesize Sertoli cells, Leydig cells, granulosa cells, and theca cells of the ovarian stroma, while ovarian small cell carcinoma of the hypercalcemic type is a type of neuroendocrine tumor. Both these tumors are uncommon, potentially malignant neoplasms in children. We report the case of a sex cord tumor with annular tubules in an 11-year-old girl and a case of small cell carcinoma of the hypercalcemic type in a 10-year-old girl...
November 2016: Korean Journal of Pediatrics
https://www.readbyqxmd.com/read/28017703/prepubertal-subchronic-exposure-to-soy-milk-and-glyphosate-leads-to-endocrine-disruption
#16
Jessica Nardi, Patricia Bonamigo Moras, Carina Koeppe, Eliane Dallegrave, Mirna Bainy Leal, Luciana Grazziotin Rossato-Grando
Lactose intolerance is characterized by low or inexistent levels of lactase, and the main treatment consists of dietary changes, especially replacing dairy milk by soy milk. Soy contains phytoestrogens, substances with known estrogenic activity, besides, glyphosate-based herbicides are extensively used in soy crops, being frequently a residue in soy beans, bringing to a concern regarding the consumption of soy-based products, especially for children in breastfeeding period with lactose intolerance. This study evaluated the pubertal toxicity of a soy milk rich feeding (supplemented or not with glyphosate, doses of 50 and 100 mg/kg) during prepubertal period in male rats...
December 23, 2016: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/28012864/germline-and-somatic-dicer1-mutations-in-familial-and-sporadic-liver-tumors
#17
Stefano Caruso, Julien Calderaro, Eric Letouzé, Jean-Charles Nault, Gabrielle Couchy, Anais Boulais, Alain Luciani, Elie-Serge Zafrani, Paulette Bioulac-Sage, Olivier Seror, Sandrine Imbeaud, Jessica Zucman-Rossi
BACKGROUND & AIMS: Growing evidence suggests that genetic predisposition significantly increases the risk of hepatocellular carcinoma (HCC), independently from the presence of other risk factors. Here, we report a novel germline DICER1 mutation associated with familial recurrent liver tumors. Next, we aimed to investigate the contribution of constitutional and somatic DICER1 mutations on HCC occurrence. METHODS: We investigated two individuals of a family that developed recurrent well-differentiated hepatocellular tumors over the years...
December 21, 2016: Journal of Hepatology
https://www.readbyqxmd.com/read/28008926/long-term-propagation-of-tree-shrew-spermatogonial-stem-cells-in-culture-and-successful-generation-of-transgenic-offspring
#18
Chao-Hui Li, Lan-Zhen Yan, Wen-Zan Ban, Qiu Tu, Yong Wu, Lin Wang, Rui Bi, Shuang Ji, Yu-Hua Ma, Wen-Hui Nie, Long-Bao Lv, Yong-Gang Yao, Xu-Dong Zhao, Ping Zheng
Tree shrews have a close relationship to primates and have many advantages over rodents in biomedical research. However, the lack of gene manipulation methods has hindered the wider use of this animal. Spermatogonial stem cells (SSCs) have been successfully expanded in culture to permit sophisticated gene editing in the mouse and rat. Here, we describe a culture system for the long-term expansion of tree shrew SSCs without the loss of stem cell properties. In our study, thymus cell antigen 1 was used to enrich tree shrew SSCs...
December 23, 2016: Cell Research
https://www.readbyqxmd.com/read/28000927/does-detection-of-ddx4-mrna-in-cell-free-seminal-plasma-represents-a-reliable-noninvasive-germ-cell-marker-in-patients-with-nonobstructive-azoospermia
#19
W Abdallah, D Hashad, R Abdelmaksoud, M M Hashad
This study aimed to investigate the potential application of DDX4 gene expression in cell-free seminal mRNA as a noninvasive biomarker for the identification of the presence of germ cells in men with nonobstructive azoospermia and to correlate this factor with testicular biopsy. Male reproductive organ-specific genes were chosen: DDX4, which is a germ cell-specific gene and transglutaminase 4, which is a prostate-specific gene that was used as a control gene. Thirty-nine azoospermic males and twenty-eight normospermic fertile males (serving as a control group) participated in the study...
December 21, 2016: Andrologia
https://www.readbyqxmd.com/read/27992447/heat-sensing-receptor-trpv1-is-a-mediator-of-thermotaxis-in-human-spermatozoa
#20
Luca De Toni, Andrea Garolla, Massimo Menegazzo, Sabina Magagna, Andrea Di Nisio, Iva Šabović, Maria Santa Rocca, Valentina Scattolini, Andrea Filippi, Carlo Foresta
The molecular bases of sperm thermotaxis, the temperature-oriented cell motility, are currently under investigation. Thermal perception relies on a subclass of the transient receptor potential [TRP] channels, whose member TRPV1 is acknowledged as the heat sensing receptor. Here we investigated the involvement of TRPV1 in human sperm thermotaxis. We obtained semen samples from 16 normozoospermic subjects attending an infertility survey programme, testis biopsies from 6 patients with testicular germ cell cancer and testis fine needle aspirates from 6 patients with obstructive azoospermia undergoing assisted reproductive technologies...
2016: PloS One
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