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https://www.readbyqxmd.com/read/28432783/gene-structure-and-expression-characteristic-of-a-novel-odorant-receptor-gene-cluster-in-the-parasitoid-wasp-microplitis-mediator-hymenoptera-braconidae
#1
S-N Wang, S Shan, Y Zheng, Y Peng, Z-Y Lu, Y-Q Yang, R-J Li, Y-J Zhang, Y-Y Guo
Odorant receptors (ORs) expressed in the antennae of parasitoid wasps are responsible for detection of various lipophilic airborne molecules. In the present study, 107 novel OR genes were identified from Microplitis mediator antennal transcriptome data. Phylogenetic analysis of the set of OR genes from M. mediator and Microplitis demolitor revealed that M. mediator OR (MmedOR) genes can be classified into different subfamilies, and the majority of MmedORs in each subfamily shared high sequence identities and clear orthologous relationships to M...
April 22, 2017: Insect Molecular Biology
https://www.readbyqxmd.com/read/28431520/infectious-agents-is-a-risk-factor-for-myxomatous-mitral-valve-degeneration-a-case-control-study
#2
Marcos Gradim Tiveron, Pablo Maria Alberto Pomerantzeff, Maria de Lourdes Higuchi, Marcia Martins Reis, Jaqueline de Jesus Pereira, Joyce Tieko Kawakami, Renata Nishiyama Ikegami, Carlos Manuel de Almeida Brandao, Fabio Biscegli Jatene
BACKGROUND: The etiology of myxomatous mitral valve degeneration (MVD) is not fully understood and may depend on time or environmental factors for which the interaction of infectious agents has not been documented. The purpose of the study is to analyze the effect of Mycoplasma pneumoniae (Mp), Chlamydophila pneumoniae (Cp) and Borrelia burgdorferi (Bb) on myxomatous mitral valve degeneration pathogenesis and establish whether increased in inflammation and collagen degradation in myxomatous mitral valve degeneration etiopathogenesis...
April 21, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28431273/chromosomal-instability-in-gastric-cancer-biology
#3
REVIEW
Saffiyeh Saboor Maleki, Christoph Röcken
Gastric cancer (GC) is the fifth most common cancer in the world and accounts for 7% of the total cancer incidence. The prognosis of GC is dismal in Western countries due to late diagnosis: approximately 70% of the patients die within 5 years following initial diagnosis. Recently, integrative genomic analyses led to the proposal of a molecular classification of GC into four subtypes, i.e.,microsatellite-instable, Epstein-Barr virus-positive, chromosomal-instable (CIN), and genomically stable GCs. Molecular classification of GC advances our knowledge of the biology of GC and may have implications for diagnostics and patient treatment...
April 18, 2017: Neoplasia: An International Journal for Oncology Research
https://www.readbyqxmd.com/read/28430631/loss-of-steroid-hormone-receptors-is-common-in-malignant-pleural-and-peritoneal-effusions-of-breast-cancer-patients-treated-with-endocrine-therapy
#4
Willemijne A M E Schrijver, Karianne Schuurman, Annelot van Rossum, Ton Peeters, Natalie Ter Hoeve, Wilbert Zwart, Paul J van Diest, Cathy B Moelans
Discordance in estrogen receptor alpha (ERα), progesterone receptor (PR), androgen receptor (AR) and human epidermal growth factor receptor 2 (HER2) status between primary breast cancers and solid distant metastases ("conversion") has been reported previously. Even though metastatic spread to the peritoneal and pleural cavities occurs frequently and is associated with high mortality, the rate of receptor conversion and the prognostic implications thereof remain elusive.We therefore determined receptor conversion in 91 effusion metastases (78 pleural, 13 peritoneal effusions) of 69 patients by immunohistochemistry (IHC) and in situ hybridization...
February 20, 2017: Oncotarget
https://www.readbyqxmd.com/read/28429762/annealing-induced-re-crystallization-in-ch3nh3pbi3-xclx-for-high-performance-perovskite-solar-cells
#5
Yingguo Yang, Shanglei Feng, Meng Li, Weidong Xu, Guangzhi Yin, Zhaokui Wang, Baoquan Sun, Xingyu Gao
Using poly(3,4-ethylenedioxythiophene):polystyrene sulfonate (PEDOT:PSS) as hole conductor, a series of inverted planar CH3NH3PbI3-xClx perovskite solar cells (PSCs) were fabricated based on perovskite annealed by an improved time-temperature dependent (TTD) procedure in a flowing nitrogen atmosphere for different time. Only after an optimum annealing time, an optimized power conversion efficiency of 14.36% could be achieved. To understand their performance dependence on annealing time, an in situ real-time synchrotron-based grazing incidence X-ray diffraction (GIXRD) was used to monitor a step-by-step gradual structure transformation from distinct mainly organic-inorganic hybrid materials into highly ordered CH3NH3PbI3 crystal during annealing...
April 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28429277/primary-ewing-sarcoma-primitive-neuroectodermal-tumor-of-the-kidney-a-clinicopathologic-study-of-23-cases
#6
Paari Murugan, Priya Rao, Pheroze Tamboli, Bogdan Czerniak, Charles C Guo
Primary Ewing sarcoma / primitive neuroectodermal tumor (ES) of the kidney is a rare neoplasm with limited clinicopathologic data. We report 23 such cases with no history of ES elsewhere in the body. The patients included 13 male and 10 female, aged 8-70 years (mean, 31 years). The average tumor size was 11.7 cm (range, 5-20 cm). Microscopic analysis showed predominantly lobular growth (n = 14), with focal papillary (n = 3), alveolar (n = 1), and hemangiopericytoma-like (n = 1) patterns. Several tumors (n = 11) exhibited robust mitotic activity (>10 mitoses/10 high-power fields)...
April 20, 2017: Pathology Oncology Research: POR
https://www.readbyqxmd.com/read/28429103/resolution-of-primary-immune-defect-in-22q11-2-deletion-syndrome
#7
Yiwa Suksawat, Achara Sathienkijkanchai, Jittima Veskitkul, Orathai Jirapongsananuruk, Nualanong Visitsunthorn, Pakit Vichyanond, Punchama Pacharn
PURPOSE: Patients with 22q11.2 deletion syndrome have a variable decrease in immunological parameters, especially regarding T cell counts. The aim of this study was to investigate immunological change over time and factors associated with immunological recovery among patients with 22q11.2 deletion syndrome. METHODS: Patients with 22q11.2 deletion syndrome diagnosed by fluorescence in situ hybridization (FISH) were studied. Immunological parameters were evaluated every 6 months until patients returned to normal...
April 20, 2017: Journal of Clinical Immunology
https://www.readbyqxmd.com/read/28428108/the-gene-copy-number-of-c-met-has-a-significant-impact-on-progression-free-survival-in-korean-patients-with-ovarian-carcinoma
#8
Wook Youn Kim, Seung-Hyuk Shim, Ho Young Jung, Meari Dong, Soo-Nyung Kim, Sun Joo Lee
The aim of this study was to compare the protein overexpression and gene copy number (GCN) of c-MET in ovarian carcinoma and to assess their prognostic roles in Korean women. MET protein expression and GCN status were determined using immunohistochemistry (IHC) and silver in situ hybridization (SISH), respectively, in 105 ovarian carcinomas comprising 63 serous, 12 mucinous, 20 clear cell, and 10 endometrioid carcinomas. All cases had been treated and followed up at a single institute in Seoul, Korea. MET protein overexpression was observed in 35 of 105 (33...
April 17, 2017: Human Pathology
https://www.readbyqxmd.com/read/28428068/comparative-analysis-of-gene-expression-profiles-for-several-migrating-cell-types-identifies-cell-migration-regulators
#9
Young-Kyung Bae, Frank Macabenta, Heather Leigh Curtis, Angelike Stathopoulos
Cell migration is an instrumental process that ensures cells are properly positioned to support the specification of distinct tissue types during development. To provide insight, we used fluorescence activated cell sorting (FACS) to isolate two migrating cell types from the Drosophila embryo: caudal visceral mesoderm (CVM) cells, precursors of longitudinal muscles of the gut, and hemocytes (HCs), the Drosophila equivalent of blood cells. ~350 genes were identified from each of the sorted samples using RNA-seq, and in situ hybridization was used to confirm expression within each cell type or, alternatively, within other interacting, co-sorted cell types...
April 17, 2017: Mechanisms of Development
https://www.readbyqxmd.com/read/28427715/multiplexed-dynamic-imaging-of-genomic-loci-by-combined-crispr-imaging-and-dna-sequential-fish
#10
Yodai Takei, Sheel Shah, Sho Harvey, Lei S Qi, Long Cai
Visualization of chromosome dynamics allows the investigation of spatiotemporal chromatin organization and its role in gene regulation and other cellular processes. However, current approaches to label multiple genomic loci in live cells have a fundamental limitation in the number of loci that can be labeled and uniquely identified. Here we describe an approach we call "track first and identify later" for multiplexed visualization of chromosome dynamics by combining two techniques: CRISPR imaging and DNA sequential fluorescence in situ hybridization...
April 17, 2017: Biophysical Journal
https://www.readbyqxmd.com/read/28427488/nr2e1-downregulation-is-involved-in-excess-retinoic-acid-induced-developmental-abnormality-in-the-mouse-brain
#11
Juan Yu, Qian Guo, Jian Bing Mu, Ting Zhang, Ren Ke Li, Jun Xie
OBJECTIVE: This study aimed to investigate the expression pattern and function of Nuclear receptor subfamily 2 group E member 1 (Nr2e1) in retinoic acid (RA)-induced brain abnormality. METHODS: The mouse model of brain abnormality was established by administering 28 mg/kg RA, and neural stem cells (NSCs) were isolated from the mouse embryo and cultured in vitro. Nr2e1 expression was detected by whole mount in situ hybridization, RT-PCR, and Western blotting. Nr2e1 function was determined by transducing Nr2e1 shRNA into NSCs, and the effect on the sonic hedgehog (Shh) signaling pathway was assessed in the cells...
March 2017: Biomedical and Environmental Sciences: BES
https://www.readbyqxmd.com/read/28427412/lack-of-interleukin-13-receptor-%C3%AE-1-delays-the-loss-of-dopaminergic-neurons-during-chronic-stress
#12
Simone Mori, Shuei Sugama, William Nguyen, Tatiana Michel, M Germana Sanna, Manuel Sanchez-Alavez, Rigo Cintron-Colon, Gianluca Moroncini, Yoshihiko Kakinuma, Pamela Maher, Bruno Conti
BACKGROUND: The majority of Parkinson's disease (PD) cases are sporadic and idiopathic suggesting that this neurodegenerative disorder is the result of both environmental and genetic factors. Stress and neuroinflammation are among the factors being investigated for their possible contributions to PD. Experiments in rodents showed that severe chronic stress can reduce the number of dopaminergic neurons in the substantia nigra pars compacta (SNc); the same cells that are lost in PD. These actions are at least in part mediated by increased oxidative stress...
April 21, 2017: Journal of Neuroinflammation
https://www.readbyqxmd.com/read/28427346/karyotypic-evolution-of-the-medicago-complex-sativa-caerulea-falcata-inferred-from-comparative-cytogenetic-analysis
#13
Feng Yu, Haiqing Wang, Yanyan Zhao, Ruijuan Liu, Quanwen Dou, Jiangli Dong, Tao Wang
BACKGROUND: Polyploidy plays an important role in the adaptation and speciation of plants. The alteration of karyotype is a significant event during polyploidy formation. The Medicago sativa complex includes both diploid (2n = 2× = 16) and tetraploid (2n = 2× = 32) subspecies. The tetraploid M. ssp. sativa was regarded as having a simple autopolyploid origin from diploid ssp. caerulea, whereas the autopolyploid origin of tetraploid ssp. falcata from diploid form ssp. falcata is still in doubt...
April 21, 2017: BMC Evolutionary Biology
https://www.readbyqxmd.com/read/28426456/expression-and-localization-of-mir-21-and-mir-126-in-mucosal-tissue-from-patients-with-inflammatory-bowel-disease
#14
Gorm Thorlacius-Ussing, Boye Schnack Nielsen, Vibeke Andersen, Kim Holmstrøm, Anders E Pedersen
BACKGROUND: microRNAs (miRNAs) are small noncoding RNAs that guide degradation of mRNA and regulate protein expression. miRNA based diagnostic biomarkers for ulcerative colitis (UC) and Crohn's disease (CD) are emerging but information about the cellular localization of many miRNAs is limited and more detailed histologic evaluation of miRNA expression patterns is needed to understand their immunobiological function. METHODS: Formalin-fixed paraffin-embedded colon biopsies from 10 patients with UC and 8 patients with CD together with 9 controls were examined by RT-qPCR and quantitative in situ hybridization (ISH)...
May 2017: Inflammatory Bowel Diseases
https://www.readbyqxmd.com/read/28426072/in-situ-hybridization-of-coox-nanoparticles-on-n-doped-graphene-through-one-step-mineralization-of-co-responsive-hydrogels
#15
Jing Ma, Xiaojuan Wang, Ting He, Minli Tan, Jun Zheng, Huiqiong Wu, Mengying Yuan, Rujuan Shen, Yi Zhang, Juan Xiang
The in situ hydrothermal mineralization for a mixture of graphene oxide (GO) and Co-responsive supramolecular hydrogels led to a hybrid graphene aerogel, doped with Co and N elements. The as-prepared aerogels exhibited a competitive half-wave potential (0.840 V, versus RHE) and peak current density (2.35 mA cm(-2)) to that of Pt/C (0.836 V, 2.23 mA cm(-2), respectively). Furthermore, they demonstrated a significantly perfect catalytic efficiency (nearly 100% of 4e(-) ORR) toward the oxygen reduction reaction (ORR)...
April 20, 2017: Dalton Transactions: An International Journal of Inorganic Chemistry
https://www.readbyqxmd.com/read/28425097/seizure-frequency-correlates-with-loss-of-dentate-gyrus-gabaergic-neurons-in-a-mouse-model-of-temporal-lobe-epilepsy
#16
Paul S Buckmaster, Emily Abrams, Xiling Wen
Epilepsy occurs in one of 26 people. Temporal lobe epilepsy is common and can be difficult to treat effectively. It can develop after brain injuries that damage the hippocampus. Multiple pathophysiological mechanisms involving the hippocampal dentate gyrus have been proposed. The present study evaluated a mouse model of temporal lobe epilepsy to test which pathological changes in the dentate gyrus correlate with seizure frequency and help prioritize potential mechanisms for further study. FVB mice (n = 127) that had experienced status epilepticus after systemic treatment with pilocarpine 31 - 61 d earlier were video-monitored for spontaneous, convulsive seizures 9 h/d every day for 24 - 36 d...
April 20, 2017: Journal of Comparative Neurology
https://www.readbyqxmd.com/read/28424873/the-changes-in-the-reproductive-barrier-between-hexaploid-wheat-triticum-aestivum-l-and-rye-secale-cereale-l-different-states-lead-to-different-fates
#17
Natalia Tikhenko, Twan Rutten, Angelika Senula, Myroslava Rubtsova, E R Joachim Keller, Andreas Börner
The changes in the reproductive barrier between hexaploid wheat ( Triticum aestivum L.) and rye ( Secale cereale L.) can be induced using in situ embryo rescue of abnormal embryos, yielding stable fertile amphidiploid plants. In intergeneric crosses between hexaploid wheat (Triticum aestivum L.) and rye (Secale cereale L.), postzygotic barriers may occur at different stages of hybrid development. One such mechanism is embryo lethality, which is genetically determined by the interaction and expression of two incompatible genes in wheat (Eml-A1) and rye (Eml-R1)...
April 19, 2017: Planta
https://www.readbyqxmd.com/read/28424781/postembedding-decalcification-of-mineralized-tissue-sections-preserves-the-integrity-of-implanted-biomaterials-and-minimizes-number-of-experimental-animals
#18
Thaqif El Khassawna, Diaa Eldin S Daghma, Sabine Stoetzel, Seemun Ray, Stefanie Kern, Deeksha Malhan, Volker Alt, Ulrich Thormann, Anja Henß, Marcus Rohnke, Annette Stengel, Fathi Hassan, Stefan Maenz, Klaus D Jandt, Michael Diefenbeck, Matthias Schumacher, Michael Gelinsky, Katrin Susanne Lips, Christian Heiss
Bone histology of decalcified or undecalcified samples depends on the investigation. However, in research each method provides different information to answer the scientific question. Decalcification is the first step after sample fixation and governs what analysis is later feasible on the sections. Besides, decalcification is favored for immunostaining and in situ hybridization. Otherwise, sample decalcification can be damaging to bone biomaterials implants that contains calcium or strontium. On the other hand, after decalcification mineralization cannot be assessed using histology or imaging mass spectrometry...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28424415/clinicopathological-features-and-outcome-of-chronic-lymphocytic-leukaemia-in-chinese-patients
#19
Thomas Sau-Yan Chan, Yuh-Shan Lee, Ilaria Del Giudice, Marilisa Marinelli, Caterina Ilari, Luciana Cafforio, Anna Guarini, Daryl Tan, Colin Phipps, Yeow-Tee Goh, William Hwang, Allan Zhi-Kai Goh, Lisa Lai-Ping Siu, Saliangi Wu, Chun-Yin Ha, Shek-Ying Lin, Chi-Hang Kwok, Chi-Kuen Lau, Kit-Fai Wong, Robin Foà, Yok-Lam Kwong, Eric Tse
Chronic lymphocytic leukaemia (CLL) is uncommon in Chinese population and its biology, genetics and treatment outcome in Chinese patients have not been comprehensively investigated. In this study, we studied the clinicopathological features and outcome of 212 Chinese patients with newly diagnosed CLL in Hong Kong and Singapore. The median age at diagnosis was 64 years. The majority of patients presented with early-stage disease (Binet stage A, 56.1%). Del(13)(q14) was the most frequent abnormality (41.7%) detected by fluorescence in situ hybridization (FISH) analysis...
March 9, 2017: Oncotarget
https://www.readbyqxmd.com/read/28424353/constrained-release-of-lamina-associated-enhancers-and-genes-from-the-nuclear-envelope-during-t-cell-activation-facilitates-their-association-in-chromosome-compartments
#20
Michael I Robson, Jose I de Las Heras, Rafal Czapiewski, Aishwarya Sivakumar, Alastair R W Kerr, Eric Schirmer
The 3D organization of the genome changes concomitantly with expression changes during hematopoiesis and immune activation. Studies have focused either on lamina-associated domains (LADs) or on topologically-associated domains (TADs), defined by preferential local chromatin interactions, and chromosome compartments, defined as higher-order interactions between TADs sharing functionally similar states. However, few studies have investigated how these affect one another. To address this, we mapped LADs using Lamin B1-DamID during Jurkat T-cell activation, finding significant genome re-organization at the nuclear periphery dominated by release of loci frequently important for T-cell function...
April 19, 2017: Genome Research
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