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In situ hybridization

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https://www.readbyqxmd.com/read/28749282/oronasal-squamous-cell-carcinomas-in-fran%C3%A3-ois-langurs-trachypithecus-francoisi
#1
John A Flanders, Michelle E Thompson, Mitzi J Palazzolo, Michael M Garner, Matthew Reed, Donna M Ialeggio, Matti Kiupel, William H Westra, Kathryn C Gamble
Squamous cell carcinomas (SCCs) are common oronasal tumors in nonhuman primates. In this study, 11 cases of oronasal SCC in François' langurs ( Trachypithecus francoisi ) are described. Five initial cases were discovered on review of the North American François' langur studbook, with a potential familial pattern observed. The studbook was used to identify related individuals, and records were requested for review. Six additional cases were documented, and samples from all cases were submitted for microscopic review, as well as polymerase chain reaction (PCR), immunohistochemistry (IHC), and in situ hybridization (ISH), for generic papillomaviruses and PCR for herpesviruses because either virus may cause SCC in humans and other nonhuman primates...
June 2017: Journal of Zoo and Wildlife Medicine: Official Publication of the American Association of Zoo Veterinarians
https://www.readbyqxmd.com/read/28748853/pingyangmycin-pretreatment-influences-the-biological-behavior-of-ocular-venous-malformation-and-relates-with-galectin-3-expression
#2
Jin Li, Jun-Bo Qiao, Qiu-Yu Liu
BACKGROUND: Galectin-3 (Gal-3) plays a role in the mechanisms underlying ocular venous malformation. We conducted this study to investigate the effect of pingyangmycin pretreatment on the Gal-3 expressions and biological behavior of ocular venous malformation. METHODS: Tissue samples were collected from 136 patients with ocular venous malformation. Patients were randomly divided into pingyangmycin (n = 69) and nonpingyangmycin group (n = 67). Patients in the pingyangmycin group received a local injection of 0...
August 5, 2017: Chinese Medical Journal
https://www.readbyqxmd.com/read/28748483/expression-of-microrna-146a-and-microrna-155-in-placental-villi-in-early-and-late-onset-preeclampsia
#3
N V Nizyaeva, G V Kulikova, M N Nagovitsyna, N E Kan, K N Prozorovskaya, A I Shchegolev, G T Sukhikh
We studied the expression of microRNA-146a and microRNA-155 in placental villi from 18 women (26-39 weeks of gestation) of reproductive age with early- or late-onset preeclampsia. The reference group consisted of women with physiological pregnancy and full-term gestation and with preterm birth after caesarian section on gestation week 26-31. MicroRNA-146a and microRNA-155 were detected by in situ hybridization with digoxigenin on paraffin sections. It was found that the expression of microRNA-146a in both syncytiotrophoblast of the intermediate villi and syncytial knots was lower at late-onset preeclampsia than at physiologic pregnancy of full-term period (p=0...
July 27, 2017: Bulletin of Experimental Biology and Medicine
https://www.readbyqxmd.com/read/28748477/chromogenic-in-situ-hybridization-methods-for-microrna-biomarker-monitoring-of-drug-safety-and-efficacy
#4
Barbara R Gould, Tina Damgaard, Boye Schnack Nielsen
Disease research and treatment development have turned to the impact and utility of microRNA. The dynamic and highly specific expression of these molecular regulators can be used to predict and monitor disease progression as well as therapeutic treatment efficacy and safety, thus aiding decisions in patient care. In situ hybridization (ISH) of biopsy material has become a routine clinical pathology procedure for monitoring gene structure, expression, and sample characterization. For ribonucleic acid (RNA), determining cell source and level of expression of these biomarkers gives insight into the cellular function and physiopathology...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28748459/tissue-sampling-and-processing-for-histopathology-evaluation
#5
Mohamed Slaoui, Anne-Laure Bauchet, Laurence Fiette
Histological procedures aim at providing good-quality sections that can be used for a light microscopic evaluation of tissue. These are applicable to identify either spontaneous or diseases-induced changes. Routinely, tissues are fixed with neutral formalin 10%, embedded in paraffin, and manually sectioned with a microtome to obtain 4-5 μm thick paraffin sections. Dewaxed sections are then stained with HE&S (hematoxylin-eosin and saffron) or can be used for other purposes (special stains, immunohistochemistry, in situ hybridization, etc...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28748349/desmoplastic-small-round-cell-tumor-evaluation-of-reverse-transcription-polymerase-chain-reaction-and-fluorescence-in-situ-hybridization-as-ancillary-molecular-diagnostic-techniques
#6
Mustafa Mohamed, David Gonzalez, Karen J Fritchie, John Swansbury, Dorte Wren, Charlotte Benson, Robin L Jones, Cyril Fisher, Khin Thway
Desmoplastic small round cell tumor (DSRCT) is a rare, biologically aggressive soft tissue neoplasm of uncertain differentiation, most often arising in the abdominal and pelvic cavities of adolescents and young adults with a striking male predominance. Histologically, it is characterized by islands of uniform small round cells in prominent desmoplastic stroma, and it has a polyimmunophenotypic profile, typically expressing WT1 and cytokeratin, desmin, and neural/neuroendocrine differentiation markers to varying degrees...
July 26, 2017: Virchows Archiv: An International Journal of Pathology
https://www.readbyqxmd.com/read/28747957/acn9-regulates-the-inflammatory-responses-in-human-bronchial-epithelial-cells
#7
Jae Hoon Jeong, Jeeyoung Kim, Jeongwoon Kim, Hye-Ryeon Heo, Jin Seon Jeong, Young-Joon Ryu, Yoonki Hong, Seon-Sook Han, Seok-Ho Hong, Seung-Joon Lee, Woo Jin Kim
BACKGROUND: Airway epithelial cells are the first line of defense, against pathogens and environmental pollutants, in the lungs. Cellular stress by cadmium (Cd), resulting in airway inflammation, is assumed to be directly involved in tissue injury, linked to the development of lung cancer, and chronic obstructive pulmonary disease (COPD). We had earlier shown that ACN9 (chromosome 7q21), is a potential candidate gene for COPD, and identified significant interaction with smoking, based on genetic studies...
July 2017: Tuberculosis and Respiratory Diseases
https://www.readbyqxmd.com/read/28747693/expression-profile-of-intestinal-stem-cell-markers-in-colitis-associated-carcinogenesis
#8
Hye Sung Kim, Cheol Lee, Woo Ho Kim, Young Hee Maeng, Bo Gun Jang
The intestinal epithelium has two distinct two stem cell populations, namely, crypt base columnar (CBC) cells and +4 cells. Several specific markers have been identified for each stem cell population. In this study, we examined the expression profiles of these markers in colitis-associated carcinogenesis (CAC) to investigate whether they can be used as biomarkers for the early detection of dysplasia. The expression of intestinal stem cell (ISC) markers was measured by real-time polymerase chain reaction during CAC that was induced by azoxymethane and dextran sodium sulfate treatment...
July 26, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28747566/pten-loss-detection-in-prostate-cancer-comparison-of-pten-immunohistochemistry-and-pten-fish-in-a-large-retrospective-prostatectomy-cohort
#9
Tamara L Lotan, Asmus Heumann, Sebastian Dwertmann Rico, Jessica Hicks, Kristen Lecksell, Christina Koop, Guido Sauter, Thorsten Schlomm, Ronald Simon
PTEN deletion is an established prognostic biomarker in prostate cancer. We compared PTEN immunohistochemistry (IHC) and PTEN fluorescence in situ hybridization (FISH) in the largest existing radical prostatectomy cohort with clinical follow-up data. There was high concordance between IHC and FISH: 93% (3098/3330) of tumors with intact PTEN IHC showed absence of PTEN gene deletion and 66% (720/1087) of cases with PTEN protein loss by IHC showed PTEN gene deletion by FISH. 84% (447/533) of cases with PTEN homozygous gene deletion had PTEN protein loss by IHC...
July 10, 2017: Oncotarget
https://www.readbyqxmd.com/read/28746978/digital-image-analysis-of-her2-immunohistochemistry-in-gastric-and-oesophageal-adenocarcinoma-a-validation-study-on-biopsies-and-surgical-specimens
#10
Timco Koopman, Geertruida H de Bock, Henk J Buikema, Maria M Smits, Maarten Louwen, Mariska Hage, Alex Lt Imholz, Bert van der Vegt
AIMS: to test the validity of diagnostics incorporating digital image analysis (DIA) for human epidermal growth factor 2 (HER2) immunohistochemistry (IHC) in gastro-oesophageal adenocarcinomas, as an alternative to current standard diagnostics using manual scoring. METHODS AND RESULTS: we included 319 consecutive gastro-oesophageal adenocarcinomas (232 biopsies and 87 surgical specimens). DIA was applied to determine HER2 IHC classification, using both standard breast cancer (BC) and modified gastro-oesophageal cancer (GEC) cutoffs...
July 26, 2017: Histopathology
https://www.readbyqxmd.com/read/28746901/enhanced-electrocatalytic-activity-of-graphene-gold-nanoparticles-hybrids-for-peroxynitrite-electrochemical-detection-on-hemin-based-electrode
#11
Beibei Wang, Xueping Ji, Jujie Ren, Ruixing Ni, Lin Wang
A simple, ultrasensitive peroxynitrite anion (ONOO(-)) electrochemical sensing platform was developed by immobilizing hemin on a density controllable electrochemically reduced graphene oxide-Au nanoparticles (ERGO-AuNPs) nanohybrids. The ERGO-AuNPs in situ nanohybrids were produced onto a glass carbon electrode (GCE) by one-step electrodeposition, the density of which could be easily controlled by electrodeposited time. The morphology of ERGO-AuNPs nanohybrids was characterized by a scanning electron microscope (SEM)...
July 15, 2017: Bioelectrochemistry
https://www.readbyqxmd.com/read/28746220/copy-number-variations-of-neurotrophic-tyrosine-receptor-kinase-3-ntrk3-may-predict-prognosis-of-ovarian-cancer
#12
Li Ge, Ning Li, Mei Liu, Ning-Zhi Xu, Ming-Rong Wang, Ling-Ying Wu
Platinum resistance is a critical barrier for clinicians to improve the survival of ovarian cancer. Our study evaluated the correlation between copy number variations (CNVs) of neurotrophic tyrosine receptor kinase 3 (NTRK3) and the prognosis of ovarian cancer, which might predict platinum resistance in ovarian cancer patients.Array comparative genomic hybridization (CGH) was used to test gene backgrounds between platinum-sensitive and platinum-resistant relapsed populations and CNVs of NTRK3 were indicated by cluster analysis...
July 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28745798/research-on-apoptotic-signaling-pathways-of-recurrent-spontaneous-abortion-caused-by-dysfunction-of-trophoblast-infiltration
#13
Q Sun, X-L Zhang
OBJECTIVE: To study the apoptotic signaling pathways of recurrent spontaneous abortion caused by dysfunction of trophoblast infiltration. PATIENTS AND METHODS: 60 patients with recurrent spontaneous abortion and normal abortion were selected consecutively as recurrent spontaneous abortion group and abortion group, respectively. Villous tissues were obtained and cell apoptosis was observed under a microscope; terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labeling (Tunel) method was used to test the apoptosis rate...
July 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28745638/localization-of-odorant-receptor-genes-in-locust-antennae-by-rna-in-situ-hybridization
#14
Xiao Xu, Yinwei You, Long Zhang
Insects have evolved sophisticated olfactory reception systems to sense exogenous chemical signals. These chemical signals are transduced by Olfactory Receptor Neurons (ORNs) housed in hair-like structures, called chemosensilla, of the antennae. On the ORNs' membranes, Odorant Receptors (ORs) are believed to be involved in odor coding. Thus, being able to identify genes localized to the ORNs is necessary to recognize OR genes, and provides a fundamental basis for further functional in situ studies. The RNA expression levels of specific ORs in insect antennae are very low, and preserving insect tissue for histology is challenging...
July 13, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28745517/it-is-all-about-location-how-to-pinpoint-microorganisms-and-their-functions-in-multispecies-biofilms
#15
Angela M Costa, Filipe J Mergulhão, Romain Briandet, Nuno F Azevedo
Multispecies biofilms represent the dominant mode of life for the vast majority of microorganisms. Bacterial spatial localization in such biostructures governs ecological interactions between different populations and triggers the overall community functions. Here, we discuss the pros and cons of fluorescence-based techniques used to decipher bacterial species patterns in biofilms at single cell level, including fluorescence in situ hybridization and the use of genetically modified bacteria that express fluorescent proteins, reporting the significant improvements of those techniques...
July 26, 2017: Future Microbiology
https://www.readbyqxmd.com/read/28744882/epstein-barr-virus-positivity-is-associated-with-angiogenesis-in-and-poorer-survival-of-patients-receiving-standard-treatment-for-classical-hodgkin-s-lymphoma
#16
Young Wha Koh, Jae-Ho Han, Dok Hyun Yoon, Cheolwon Suh, Jooryung Huh
Epstein-Barr virus (EBV) is a significant contributor to the development of classical Hodgkin's lymphoma (cHL). Recent studies have documented associations between angiogenesis and EBV-associated malignancies. No study has yet examined the associations among, and prognostic implications of, EBV infection, vascular endothelial growth factor (VEGF) expression, and microvessel density (MVD) in cHL patients. Diagnostic tissues from 135 cHL patients treated with adriamycin, bleomycin, vinblastine, and dacarbazine (ABVD) were retrospectively evaluated by in situ hybridization of EBV-encoded small RNA (EBER) and immunohistochemical staining for VEGF and CD31 (a measure of MVD)...
July 26, 2017: Hematological Oncology
https://www.readbyqxmd.com/read/28743832/a-nomogram-to-predict-her2-status-in-breast-cancer-patients-with-her2-borderline-disease-as-determined-via-immunohistochemistry
#17
Qianqian Guo, Kai Chen, Xiaojie Lin, Yi Su, Rui Xu, Yan Dai, Chang Qiu, Xue Song, Siying Mao, Qianjun Chen
This study aimed to develop a nomogram to predict fluorescence in situ hybridization (FISH) assay results for HER2-borderline breast cancer as determined via immunohistochemistry (IHC) among patients in China. We reviewed a database of breast cancer patients diagnosed between January 2007 and April 2013 at our institutions. We used logistic regression to develop a nomogram and we used receiver operating characteristic curve analysis and calibration plots to validate our nomogram. In total, 1138, 301 and 344 patients had IHC-determined HER2-negative, HER2-borderline and HER2-positive disease, respectively...
July 17, 2017: Oncotarget
https://www.readbyqxmd.com/read/28743306/detection-of-22-common-leukemic-fusion-genes-using-a-single-step-multiplex-qrt-pcr-based-assay
#18
Xiaodong Lyu, Xianwei Wang, Lina Zhang, Zhenzhu Chen, Yu Zhao, Jieying Hu, Ruihua Fan, Yongping Song
BACKGROUND: Fusion genes generated from chromosomal translocation play an important role in hematological malignancies. Detection of fusion genes currently employ use of either conventional RT-PCR methods or fluorescent in situ hybridization (FISH), where both methods involve tedious methodologies and require prior characterization of chromosomal translocation events as determined by cytogenetic analysis. In this study, we describe a real-time quantitative reverse transcription PCR (qRT-PCR)-based multi-fusion gene screening method with the capacity to detect 22 fusion genes commonly found in leukemia...
July 25, 2017: Diagnostic Pathology
https://www.readbyqxmd.com/read/28743163/alk-and-ros1-testing-on-lung-cancer-cytologic-samples-perspectives
#19
REVIEW
Pasquale Pisapia, Maria D Lozano, Elena Vigliar, Claudio Bellevicine, Francesco Pepe, Umberto Malapelle, Giancarlo Troncone
Cytologic sampling is the mainstay of diagnosing advanced lung cancer. Moreover, to select patients for personalized first-line or second-line treatment, epidermal growth factor receptor (EGFR) mutations and anaplastic lymphoma kinase (ALK) and c-ros oncogene 1 (ROS1) rearrangements are tested on cytologic preparations. Commercially available fluorescence in situ hybridization (FISH) and immunocytochemistry (ICC) assays have primarily been used for the identification of cells harboring ALK or ROS1 gene fusions on histologic rather than cytologic preparations...
July 25, 2017: Cancer
https://www.readbyqxmd.com/read/28743084/graphene-oxide-gold-nanorods-based-multiple-assisted-electrochemiluminescence-signal-amplification-strategy-for-sensitive-detection-of-prostate-specific-antigen
#20
Jun-Tao Cao, Jiu-Jun Yang, Li-Zhen Zhao, Yu-Ling Wang, Hui Wang, Yan-Ming Liu, Shu-Hui Ma
A novel and competitive electrochemiluminescence (ECL) aptasensor for prostate specific antigen (PSA) assay was constructed using gold nanorods functionalized graphene oxide (GO@AuNRs) multilabeled with glucose oxidase (GOD) and streptavidin (SA) toward luminol-based ECL system. A strong initial ECL signal was achieved by electrodeposited gold (DpAu) on the electrode because of gold nanoparticles (AuNPs) motivating the luminol ECL signal. The signal probes prepared by loading GOD and SA-biotin-DNA on GO@AuNRs were used for achieving multiple signal amplification...
July 20, 2017: Biosensors & Bioelectronics
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