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https://www.readbyqxmd.com/read/28108956/post-implantation-fate-of-adipogenic-induced-mesenchymal-stem-cells-on-type-i-collagen-scaffold-in-a-rat-model
#1
Balu Venugopal, Francis B Fernandez, V S Harikrishnan, Annie John
Regenerative medicine via its application in soft tissue reconstruction through novel methods in adipose tissue engineering (ATE) has gained remarkable attention and investment despite simultaneous reports on clinical incidence of graft resorption and impaired vascularization. The underlying malaise here once identified may play a critical role in optimizing implant function. Our work attempts to determine the fate of donor cells and the implant in recipient micro environment using adipose-derived mesenchymal stem cells (ASCs) on a type I collagen sponge, an established scaffold for ATE...
February 2017: Journal of Materials Science. Materials in Medicine
https://www.readbyqxmd.com/read/28108848/glucagon-like-peptide-1-is-co-localized-with-neurotensin-in-the-chicken-ileum
#2
Kei Nishimura, Kohzy Hiramatsu, Takafumi Watanabe, Kazumi Kita
Glucagon-like peptide (GLP)-1 and neurotensin (NT) are distributed throughout the chicken ileum. Here, we attempt to determine if GLP-1 and NT co-localize in the chicken ileum by using immunofluorescence, immunocytochemistry and in situ hybridization techniques. Three types of enteroendocrine cells, GLP-1(+)/NT(+), GLP-1(+)/NT(-) and GLP-1(-)/NT(+) cells, were detected in the mucosal epithelium by the double immunofluorescence method. The ratio of GLP-1(+)/NT(+) cells at the crypts in the distal ileum was significantly higher than that in the proximal ileum...
January 20, 2017: Cell and Tissue Research
https://www.readbyqxmd.com/read/28108587/meiotic-crossing-over-in-maize-knob-heterochromatin
#3
Stephen M Stack, Lindsay A Shearer, Leslie Lohmiller, Lorinda K Anderson
There is ample evidence that crossing over is suppressed in heterochromatin associated with centromeres and nucleolus organizers (NORs). This characteristic has been attributed to all heterochromatin, but the generalization may not be justified. To investigate the relation of crossing over to heterochromatin that is not associated with centromeres or NORs, we used a combination of fluorescence in situ hybridization of the maize 180 bp knob repeat to show the locations of knob heterochromatin and fluorescent immunolocalization of MLH1 protein and AFD1 protein to show the locations of MLH1 foci on maize synaptonemal complexes (SCs ~ pachytene chromosomes)...
January 20, 2017: Genetics
https://www.readbyqxmd.com/read/28108289/negative-feedback-regulation-between-microrna-let-7g-and-lox-1-mediated-hypoxia-induced-pasmcs-proliferation
#4
Wei-Fang Zhang, Tian-Tian Zhu, You-Wen Xiong, Ai-Zhen Xiong, Xiao-Yue Ge, Chang-Ping Hu, Zheng Zhang
BACKGROUND: Pulmonary hypertension (PH) is a proliferative disorder associated with enhanced proliferation and suppressed apoptosis of pulmonary artery smooth muscle cells (PASMCs). Our lately study demonstrated that let-7g inhibited hypoxia-induced proliferation of PASMCs via repressed c-myc-Bmi-1-p16 signaling pathway. However, the upstream of let-7g has not yet been fully defined. Previous studies have shown that LOX-1, a target of let-7g, could also regulate the expression of let-7g in human aortic endothelial cells...
January 17, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28107429/deficient-cholesterol-esterification-in-plasma-of-apoc2-knockout-zebrafish-and-familial-chylomicronemia-patients
#5
Chao Liu, Daniel Gaudet, Yury I Miller
Hypertriglyceridemia is an independent risk factor for cardiovascular disease. Apolipoprotein C-II (APOC2) is an obligatory cofactor for lipoprotein lipase (LPL), the major enzyme catalyzing plasma triglyceride hydrolysis. We have created an apoc2 knockout zebrafish model, which mimics the familial chylomicronemia syndrome (FCS) in human patients with a defect in the APOC2 or LPL gene. In this study, we measured plasma levels of free cholesterol (FC) and cholesterol esters (CE) and found that apoc2 mutant zebrafish have a significantly higher FC to CE ratio (FC/CE), when compared to the wild type...
2017: PloS One
https://www.readbyqxmd.com/read/28107180/identification-of-micrornas-implicated-in-the-late-differentiation-stages-of-normal-b-cells-suggests-a-central-role-for-mirna-targets-zeb1-and-tp53
#6
Giorgio Malpeli, Stefano Barbi, Simonetta Zupo, Gabriele Tosadori, Giovanni Scardoni, Anna Bertolaso, Silvia Sartoris, Stefano Ugel, Caterina Vicentini, Matteo Fassan, Annalisa Adamo, Mauro Krampera, Maria Teresa Scupoli, Carlo Maria Croce, Aldo Scarpa
In the late B cell differentiation stages, miRNAs expression modifications promoting or inhibiting key pathways are only partially defined. We isolated 29 CD19+ human B cell samples at different stages of differentiation: B cells from peripheral blood; naïve, germinal center (GC) and subepithelial (SE) B cells from tonsils. SE cells were further split in activated and resting B cell. The miRNA expression profile of these B cells was assessed by microarray analysis and selected miRNAs were validated by quantitative RT-PCR and in situ hybridization on normal tonsils...
January 17, 2017: Oncotarget
https://www.readbyqxmd.com/read/28106924/incidence-clinicopathologic-features-and-fusion-transcript-landscape-of-translocation-renal-cell-carcinomas
#7
Marion Classe, Gabriel G Malouf, Xiaoping Su, Hui Yao, Erika J Thompson, Denaha J Doss, Valérie Grégoire, Julien Lenobin, Jean-Christophe Fantoni, Hélène Sudour-Bonnange, David Khayat, Sébastien Aubert, Nizar M Tannir, Xavier Leroy
AIMS: Translocation renal cell carcinoma (tRCC) is a rare subtype of kidney tumour characterized by translocations involving the transcription factor TFE3 or TFEB. tRCC was introduced into the World Health Organization classification in 2004, but much is still unknown about the natural history, clinicopathologic features, and outcomes of the disease. The aim of this study was to describe the landscape of fusion transcript in a large single-institution series of FISH confirmed tRCCs and then to confront it to morphological and clinical data...
January 20, 2017: Histopathology
https://www.readbyqxmd.com/read/28106838/molecular-characterization-of-voltage-gated-sodium-channels-and-their-relations-with-paralytic-shellfish-toxin-bioaccumulation-in-the-pacific-oyster-crassostrea-gigas
#8
Floriane Boullot, Justine Castrec, Adeline Bidault, Natanael Dantas, Laura Payton, Mickael Perrigault, Damien Tran, Zouher Amzil, Pierre Boudry, Philippe Soudant, Hélène Hégaret, Caroline Fabioux
Paralytic shellfish toxins (PST) bind to voltage-gated sodium channels (Nav) and block conduction of action potential in excitable cells. This study aimed to (i) characterize Nav sequences in Crassostrea gigas and (ii) investigate a putative relation between Nav and PST-bioaccumulation in oysters. The phylogenetic analysis highlighted two types of Nav in C. gigas: a Nav1 (CgNav1) and a Nav2 (CgNav2) with sequence properties of sodium-selective and sodium/calcium-selective channels, respectively. Three alternative splice transcripts of CgNav1 named A, B and C, were characterized...
January 19, 2017: Marine Drugs
https://www.readbyqxmd.com/read/28106800/frequency-and-pathological-phenotype-of-bovine-astrovirus-ch13-neuros1-infection-in-neurologically-diseased-cattle-towards-assessment-of-causality
#9
Senija Selimovic-Hamza, Céline L Boujon, Monika Hilbe, Anna Oevermann, Torsten Seuberlich
Next-generation sequencing (NGS) has opened up the possibility of detecting new viruses in unresolved diseases. Recently, astrovirus brain infections have been identified in neurologically diseased humans and animals by NGS, among them bovine astrovirus (BoAstV) CH13/NeuroS1, which has been found in brain tissues of cattle with non-suppurative encephalitis. Only a few studies are available on neurotropic astroviruses and a causal relationship between BoAstV CH13/NeuroS1 infections and neurological disease has been postulated, but remains unproven...
January 18, 2017: Viruses
https://www.readbyqxmd.com/read/28106517/traditional-invasive-and-synchrotron-based-non-invasive-assessments-of-3d-printed-hybrid-cartilage-constructs
#10
Adeola Deborah Olubamiji, Ning Zhu, Tuanjie Chang, Chijioke Nwankwo, Zohreh Izadifar, Ali Honaramooz, Daniel Chen, B Frank Eames
Three-dimensional (3D)-printed constructs made of polycaprolactone (PCL) and chondrocyte-impregnated alginate hydrogel (hybrid cartilage constructs) mimic the biphasic nature of articular cartilage, offering promise for cartilage tissue engineering (CTE) applications. However, the regulatory pathway for medical device development requires validation of such constructs through in vitro bench tests and in vivo preclinical examinations premarket approval. Furthermore, non-invasive imaging techniques are required for effective evaluation of the progress of these cartilage constructs, especially when implanted in animal models or human subjects...
January 20, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28106373/preparation-of-reduced-graphene-oxide-zno-hybrid-cathode-interlayer-using-in-situ-thermal-reduction-annealing-for-interconnecting-nanostructure-and-its-effect-on-organic-solar-cell
#11
Ding Zheng, Wei Huang, Pu Fan, Yifan Zheng, Jiang Huang, Junsheng Yu
A novel hybrid cathode interlayer (CIL) consisting of reduced graphene oxide and zinc oxide (ZnO) is realized in the inverted organic solar cells (OSCs). Dual-nozzle spray coating system and facile one step in-situ thermal reduction/annealing (ITR/ITA) method are introduced to precisely control the components of the CIL, assemble ZnO with graphene oxide, and reduce graphene oxide into in-situ thermal reduced graphene oxide (IT-RGO), simultaneously. The ZnO:IT-RGO hybrid CIL shows high electric conductivity, interconnecting nanostructure and matched energy level, which leads to a significant enhancement in the power conversion efficiency from 6...
January 20, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28105851/in-situ-hybridization-and-promoter-analysis-reveal-that-cotton-leaf-curl-multan-betasatellite-localizes-in-the-phloem
#12
O Eini, M S Rasheed, J W Randles
Begomoviruses (the family Geminiviridae) have either a monopartite or a bipartite (DNA A and DNA B) single-stranded DNA genome. DNA B contains the open reading frame for movement protein and enables some bipartite begomoviruses to invade non-phloem tissues, whereas monopartite begomoviruses are limited to the phloem. Betasatellite DNA replicates in association with some monopartite begomoviruses to produce severe symptoms and enhance replication of helper virus in some hosts. The cotton leaf curl Multan betasatellite (CLCuMuB) has been shown to substitute for the DNA B of a bipartite begomovirus by permitting systemic infection, but the tissue tropism and the role of betasatellites in releasing monopartite begomoviruses from the phloem to adjacent tissue has not been described...
January 19, 2017: Acta Virologica
https://www.readbyqxmd.com/read/28105837/hrp-mimicking-dnazyme-catalyzed-in-situ-generation-of-polyaniline-to-assist-signal-amplification-for-ultrasensitive-surface-plasmon-resonance-biosensing
#13
Haiyin Li, Jiafu Chang, Ting Hou, Feng Li
It is well-known that the horseradish peroxidase- (HRP-) mimicking DNAzyme, namely, hemin/G-quadruplex, can effectively catalyze the polymerization of aniline to form DNA-guided polyaniline. Meanwhile, polyaniline exhibits extraordinary electrical, electrochemical, and redox properties, as well as excellent SPR signal-enhancing ability. Herein, we report a novel ultrasensitive surface plasmon resonance (SPR) biosensor based on HRP-mimicking DNAzyme-catalyzed in situ formation of polyaniline for signal amplification, using bleomycin (BLM) as the proof-of-concept analyte...
January 3, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28105525/characterization-of-the-cell-polarity-gene-crumbs-during-the-early-development-and-maintenance-of-the-squid-vibrio-light-organ-symbiosis
#14
Suzanne M Peyer, Elizabeth A C Heath-Heckman, Margaret J McFall-Ngai
The protein Crumbs is a determinant of apical-basal cell polarity and plays a role in apoptosis of epithelial cells and their protection against photodamage. Using the squid-vibrio system, a model for development of symbiotic partnerships, we examined the modulation of the crumbs gene in host epithelial tissues during initiation and maintenance of the association. The extracellular luminous symbiont Vibrio fischeri colonizes the apical surfaces of polarized epithelia in deep crypts of the Euprymna scolopes light organ...
January 20, 2017: Development Genes and Evolution
https://www.readbyqxmd.com/read/28105214/detection-value-of-tumor-cells-in-cerebrospinal-fluid-in-the-diagnosis-of-meningeal-metastasis-from-lung-cancer-by-immuno-fish-technology
#15
Yuan Lv, Ning Mu, Chunhua Ma, Rong Jiang, Qiaoli Wu, Jinduo Li, Bin Wang, Liwei Sun
To investigate the detection value of tumor cells in cerebrospinal fluid (CSF) in the adjuvant diagnosis of meningeal metastasis from lung cancer by immunofluorescence in situ hybridization (immuno-FISH) technology. The circulating tumor cells (CTCs) in the CSF of 16 patients with meningeal metastasis from lung cancer and 8 with non-tumor diseases in the brain were detected using immuno-FISH technology. The diagnosis of meningeal metastasis from lung cancer was based on neurological symptoms, enhanced magnetic resonance imaging (MRI) scans and CSF cytological examination...
December 2016: Oncology Letters
https://www.readbyqxmd.com/read/28105172/her2-gene-amplification-in-patients-with-prostate-cancer-evaluating-a-cish-based-method
#16
Nazanin Sharifi, Arash Salmaninejad, Samira Ferdosi, Abolfazl Nesaei Bajestani, Malihe Khaleghiyan, Mehrdad Asghari Estiar, Mansour Jamali, Mohammad Reza Nowroozi, Abbas Shakoori
Prostate cancer (PCa) is one of the most widespread malignancies in the world. The role of the human epidermal growth factor receptor 2 (HER2) in the pathogenesis and progression of human PCa remains poorly understood. In contradiction with breast cancer, studies on HER2 overexpression and gene amplification in PCa have produced varying results, although the HER2 oncogene has been implicated in the biology of numerous tumor types, and serves as a prognostic marker and therapeutic target in breast cancer. Technical challenges are considered the main reasons for data discrepancies...
December 2016: Oncology Letters
https://www.readbyqxmd.com/read/28105150/ovarian-metastasis-from-non-small-cell-lung-cancer-with-alk-and-egfr-mutations-a-report-of-two-cases
#17
Rosemary T Mushi, Yumei Yang, Qian Cai, Ruiguang Zhang, Gang Wu, Xiaorong Dong
Ovarian metastasis from non-small cell lung cancer (NSCLC) is a rare condition. The current study presents the cases of 2 female patients aged 38 and 47 years old, respectively, who were initially diagnosed with NSCLC adenocarcinoma on histology. Both patients initially presented with chest pain and a cough, and subsequently developed ovarian metastases following multiple treatments. The 38-year old patient exhibited an epidermal growth factor receptor mutation, confirmed by scorpion/amplified refractory mutation system analysis from a lung biopsy...
December 2016: Oncology Letters
https://www.readbyqxmd.com/read/28105149/t-14-18-q32-q21-in-chronic-lymphocytic-leukemia-patients-report-of-two-cases-and-a-literature-review
#18
Weifeng Chen, Yi Miao, Rong Wang, Yujie Wu, Hairong Qiu, Wei Xu, Jianyong Li, Lei Fan, Xin Xu
The chromosomal abnormality t(14;18)(q32;q21) is most commonly associated with germinal center-derived B-cell lymphomas, particularly follicular lymphoma (FL). Generally, it is considered a hallmark of FL. The t(14;18)(q32;q21) translocation is rare in chronic lymphocytic leukemia (CLL) and its prognostic significance remains unclear. In the present study, two cases of CLL with t(14;18)(q32;q21) were diagnosed using conventional cytogenetic analysis and fluorescence in situ hybridization. Both patients presented with leukemia and the morphological features and immunophenotypes were typical of CLL...
December 2016: Oncology Letters
https://www.readbyqxmd.com/read/28104824/gnrh-mrna-expression-in-the-brain-of-cooperatively-breeding-female-damaraland-mole-rats
#19
Cornelia Voigt, Nc Bennett
The Damaraland mole-rat (Fukomys damarensis) is a eusocial, subterranean rodent, in which breeding is limited to a single reproductive pair within each colony. Non-reproductive females, while in the confines of the colony, exhibit socially-induced infertility. Anovulation is thought to be caused by a disruption in the normal gonadotropin-releasing hormone (GnRH) secretion from the hypothalamus. In order to assess whether social suppression is associated with altered GnRH mRNA expression in the brain we investigated the distribution and gene expression levels by means of in situ hybridization in female breeders and non-breeders from field captured colonies of the Damaraland mole-rat...
January 19, 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28104666/inter-laboratory-quantification-of-bacteria-and-archaea-in-deeply-buried-sediments-of-the-baltic-sea-iodp-expedition-347
#20
Joy Buongiorno, Stephanie Turner, Gordon Webster, Masanori Asai, Alexander K Shumaker, Taylor Roy, Andrew Weightman, Axel Schippers, Karen G Lloyd
Two common quantification methods for subseafloor microorganisms are catalyzed reporter deposition fluorescence in situ hybridization (CARD-FISH) and quantitative PCR (qPCR). Using these methods, we quantified Bacteria and Archaea in Baltic Sea basin sediments (IODP Exp. 347) down to 90 mbsf, testing the following hypotheses in an inter-laboratory comparison: 1) proteinase K permeabilization of Archaeal cell walls increases CARD-FISH accuracy, and 2) qPCR varies by more than an order of magnitude between laboratories using similar protocols...
January 18, 2017: FEMS Microbiology Ecology
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