keyword
MENU ▼
Read by QxMD icon Read
search

Rrna

keyword
https://www.readbyqxmd.com/read/29220820/application-of-an-in-situ-soil-sampler-for-assessing-subsurface-biogeochemical-dynamics-in-a-diesel-contaminated-coastal-site-during-soil-flushing-operations
#1
Man Jae Kwon, Edward J O'Loughlin, Baknoon Ham, Yunho Hwang, Moojoon Shim, Soonjae Lee
Subsurface biogeochemistry and contaminant dynamics during the remediation of diesel-contamination by in-situ soil flushing were investigated at a site located in a coastal region. An in-situ sampler containing diesel-contaminated soils separated into two size fractions (<0.063- and <2-mm) was utilized in two monitoring wells: DH1 (located close to the injection and extraction wells for in-situ soil flushing) and DH2 (located beyond sheet piles placed to block the transport of leaked diesel). Total petroleum hydrocarbon (TPH) concentrations and biogeochemical properties were monitored both in soil and groundwater for six months...
December 5, 2017: Journal of Environmental Management
https://www.readbyqxmd.com/read/29220800/exposure-to-traffic-related-air-pollution-and-the-composition-of-the-gut-microbiota-in-overweight-and-obese-adolescents
#2
Tanya L Alderete, Roshonda B Jones, Zhanghua Chen, Jeniffer S Kim, Rima Habre, Frederick Lurmann, Frank D Gilliland, Michael I Goran
BACKGROUND: Traffic-related air pollution (TRAP) exposure has been linked to type 2 diabetes and metabolic dysfunction in humans. Animal studies suggest that air pollutants may alter the composition of the gut microbiota, which may negatively impact metabolic health through changes in the composition and/or function of the gut microbiome. OBJECTIVES: The primary aim of this study was to determine whether elevated TRAP exposure was correlated with gut bacterial taxa in overweight and obese adolescents from the Meta-AIR (Metabolic and Asthma Incidence Research) study...
December 5, 2017: Environmental Research
https://www.readbyqxmd.com/read/29220785/metagenomic-exploration-reveals-a-marked-change-in-the-river-resistome-and-mobilome-after-treated-wastewater-discharges
#3
Itziar Lekunberri, José Luis Balcázar, Carles M Borrego
Mobile genetic elements (MGEs) are key agents in the spread of antibiotic resistance genes (ARGs) across environments. Here we used metagenomics to compare the river resistome (collection of all ARGs) and mobilome (e.g., integrases, transposases, integron integrases and insertion sequence common region "ISCR" elements) between samples collected upstream (n = 6) and downstream (n = 6) of an urban wastewater treatment plant (UWWTP). In comparison to upstream metagenomes, downstream metagenomes showed a drastic increase in the abundance of ARGs, as well as markers of MGEs, particularly integron integrases and ISCR elements...
December 5, 2017: Environmental Pollution
https://www.readbyqxmd.com/read/29218079/vegf-c-mediated-enhancement-of-lymphatic-drainage-reduces-intestinal-inflammation-by-regulating-il-9-il-17-balance-and-improving-gut-microbiota-in-experimental-chronic-colitis
#4
Xiaolei Wang, Jin Zhao, Li Qin
BACKGROUND: Inflammation-associated lymphangiogenesis (IAL) induced by vascular endothelial growth factor (VEGF)-C/VEGF receptor-3 (VEGFR-3) pathway plays a crucial role in chronic intestinal inflammation. This study aimed to investigate the effects of VEGF-C mediated enhancement of lymphatic drainage on the intestinal inflammation in experimental chronic colitis (CC) and the potential mechanism was explored. METHODS: Mouse CC model was established by three cycles of 2% DSS administration for 5 days following water administration for 5 days...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/29217369/influence-of-the-intestinal-microbiota-on-the-immunogenicity-of-oral-rotavirus-vaccine-given-to-infants-in-south-india
#5
Edward P K Parker, Ira Praharaj, Anna Zekavati, Robin P Lazarus, Sidhartha Giri, Darwin J Operario, Jie Liu, Eric Houpt, Miren Iturriza-Gómara, Beate Kampmann, Jacob John, Gagandeep Kang, Nicholas C Grassly
Oral rotavirus vaccines have consistently proven to be less immunogenic among infants in developing countries. Discrepancies in the intestinal microbiota, including a greater burden of enteropathogens and an altered commensal community composition, may contribute to this trend by inhibiting the replication of vaccine viruses. To test this possibility, we performed a nested case-control study in Vellore, India, in which we compared the intestinal microbiota of infants who responded serologically or not after two doses of Rotarix delivered at 6 and 10 weeks of age as part of a clinical trial (CTRI/2012/05/002677)...
December 4, 2017: Vaccine
https://www.readbyqxmd.com/read/29216634/lysine-restriction-affects-feed-intake-and-amino-acid-metabolism-via-gut-microbiome-in-piglets
#6
Jie Yin, Hui Han, Yuying Li, Zhaojin Liu, Yurong Zhao, Rejun Fang, Xingguo Huang, Jie Zheng, Wenkai Ren, Fei Wu, Gang Liu, Xin Wu, Kai Wang, Liping Sun, Chunyong Li, Tiejun Li, Yulong Yin
BACKGROUND/AIMS: Our previous reports suggested that dietary supplementation with lysine influenced intestinal absorption and metabolism of amino acids. In this study, we further investigated the effect of lysine restriction (30%) on feed intake and we also tested the hypothesis that gut microbiome contributed to the potential mechanism of lysine restriction-mediated feeding behavior. Here, we profiled gut microbial communities by sequencing 16S ribosomal ribonucleic acid (rRNA) genes from gut samples as well as growth performance, serum hormones, and intestinal lysine transport in a piglet model...
December 6, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29216573/nitrogen-removal-performance-and-microbial-community-changes-in-subsurface-wastewater-infiltration-systems-swiss-at-low-temperature-with-different-bioaugmentation-strategies
#7
Chunjing Liu, Jianzhi Xie, Manli Song, Zhiling Gao, Dongxing Zheng, Xia Liu, Guohui Ning, Xu Cheng, Harry Bruning
Poor nitrogen removal efficiency (mainly nitrate, NO3--N) at low temperatures strongly limits application of subsurface wastewater infiltration systems (SWISs). Seven psychrophilic strains (heterotrophic nitrifying bacteria and aerobic denitrifying bacteria) were isolated and added to SWISs to investigate the effect of embedding and direct-dosing bioaugmentation strategies on sewage treatment performance at low temperature. Both bioaugmentation strategies improved ammonium (NH4+-N) removal efficiencies, and the embedding strategy also exhibited satisfactory NO3--N and total nitrogen (TN) removal efficiencies...
December 2, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/29216488/microbial-community-composition-and-pahs-removal-potential-of-indigenous-bacteria-in-oil-contaminated-sediment-of-taean-coast-korea
#8
Dong Wan Lee, Hanbyul Lee, Aslan Hwanhwi Lee, Bong-Oh Kwon, Jong Seong Khim, Un Hyuk Yim, Beom Seok Kim, Jae-Jin Kim
The tidal flats near Sinduri beach in Taean, Korea, have been severely contaminated by heavy crude oils due to the Korea's worst oil spill accident, say the Hebei Spirit Oil Spill, in 2007. Crude oil compounds, including polycyclic aromatic hydrocarbons (PAHs), pose significant environmental damages due to their wide distribution, persistence, high toxicity, mutagenicity, and carcinogenicity. Microbial community of Sinduri beach sediments samples was analyzed by metagenomic data with 16S rRNA gene amplicons...
December 4, 2017: Environmental Pollution
https://www.readbyqxmd.com/read/29216281/complete-mitochondrial-genome-of-zeugodacus-tau-insecta-tephritidae-and-differentiation-of-z-tau-species-complex-by-mitochondrial-cytochrome-c-oxidase-subunit-i-gene
#9
Hoi-Sen Yong, Sze-Looi Song, Phaik-Eem Lim, Praphathip Eamsobhana
The tephritid fruit fly Zeugodacus tau (Walker) is a polyphagous fruit pest of economic importance in Asia. Studies based on genetic markers indicate that it forms a species complex. We report here (1) the complete mitogenome of Z. tau from Malaysia and comparison with that of China as well as the mitogenome of other congeners, and (2) the relationship of Z. tau taxa from different geographical regions based on sequences of cytochrome c oxidase subunit I gene. The complete mitogenome of Z. tau had a total length of 15631 bp for the Malaysian specimen (ZT3) and 15835 bp for the China specimen (ZT1), with similar gene order comprising 37 genes (13 protein-coding genes-PCGs, 2 rRNA genes, and 22 tRNA genes) and a non-coding A + T-rich control region (D-loop)...
2017: PloS One
https://www.readbyqxmd.com/read/29216246/draft-genome-sequence-of-actinotignum-schaalii-dsm-15541t-genetic-insights-into-the-lifestyle-cell-fitness-and-virulence
#10
Atteyet F Yassin, Stefan Langenberg, Marcel Huntemann, Alicia Clum, Manoj Pillay, Krishnaveni Palaniappan, Neha Varghese, Natalia Mikhailova, Supratim Mukherjee, T B K Reddy, Chris Daum, Nicole Shapiro, Natalia Ivanova, Tanja Woyke, Nikos C Kyrpides
The permanent draft genome sequence of Actinotignum schaalii DSM 15541T is presented. The annotated genome includes 2,130,987 bp, with 1777 protein-coding and 58 rRNA-coding genes. Genome sequence analysis revealed absence of genes encoding for: components of the PTS systems, enzymes of the TCA cycle, glyoxylate shunt and gluconeogensis. Genomic data revealed that A. schaalii is able to oxidize carbohydrates via glycolysis, the nonoxidative pentose phosphate and the Entner-Doudoroff pathways. Besides, the genome harbors genes encoding for enzymes involved in the conversion of pyruvate to lactate, acetate and ethanol, which are found to be the end products of carbohydrate fermentation...
2017: PloS One
https://www.readbyqxmd.com/read/29215639/the-ribosome-moves-rna-mechanics-and-translocation
#11
Harry F Noller, Laura Lancaster, Jie Zhou, Srividya Mohan
During protein synthesis, mRNA and tRNAs must be moved rapidly through the ribosome while maintaining the translational reading frame. This process is coupled to large- and small-scale conformational rearrangements in the ribosome, mainly in its rRNA. The free energy from peptide-bond formation and GTP hydrolysis is probably used to impose directionality on those movements. We propose that the free energy is coupled to two pawls, namely tRNA and EF-G, which enable two ratchet mechanisms to act separately and sequentially on the two ribosomal subunits...
December 7, 2017: Nature Structural & Molecular Biology
https://www.readbyqxmd.com/read/29215567/are-pericentric-inversions-reorganizing-wedge-shell-genomes
#12
Daniel García-Souto, Concepción Pérez-García, Juan J Pasantes
Wedge shells belonging to the Donacidae family are the dominant bivalves in exposed beaches in almost all areas of the world. Typically, two or more sympatric species of wedge shells differentially occupy intertidal, sublittoral, and offshore coastal waters in any given locality. A molecular cytogenetic analysis of two sympatric and closely related wedge shell species, Donax trunculus and Donax vittatus, was performed. Results showed that the karyotypes of these two species were both strikingly different and closely alike; whilst metacentric and submetacentric chromosome pairs were the main components of the karyotype of D...
December 7, 2017: Genes
https://www.readbyqxmd.com/read/29215213/identifying-protist-consumers-of-photosynthetic-picoeukaryotes-in-the-surface-ocean-using-stable-isotope-probing
#13
William D Orsi, Susanne Wilken, Javier Del Campo, Thierry Heger, Erick James, Thomas A Richards, Patrick J Keeling, Alexandra Z Worden, Alyson E Santoro
Photosynthetic picoeukaryotes contribute a significant fraction of primary production in the upper ocean. Micromonas pusilla is an ecologically relevant photosynthetic picoeukaryote, abundantly and widely distributed in marine waters. Grazing by protists may control the abundance of picoeukaryotes such as M. pusilla, but the diversity of the responsible grazers is poorly understood. To identify protists consuming photosynthetic picoeukaryotes in a productive North Pacific Ocean region, we amended seawater with living 15 N, 13 C-labeled M...
December 7, 2017: Environmental Microbiology
https://www.readbyqxmd.com/read/29215200/mir-424-5p-reduces-ribosomal-rna-and-protein-synthesis-in-muscle-wasting
#14
Martin Connolly, Richard Paul, Roser Farre-Garros, Samantha A Natanek, Susannah Bloch, Jen Lee, Jose P Lorenzo, Harnish Patel, Cyrus Cooper, Avan A Sayer, Stephen J Wort, Mark Griffiths, Michael I Polkey, Paul R Kemp
BACKGROUND: A loss of muscle mass occurs as a consequence of a range of chronic and acute diseases as well as in older age. This wasting results from an imbalance of protein synthesis and degradation with a reduction in synthesis and resistance to anabolic stimulation often reported features. Ribosomes are required for protein synthesis, so changes in the control of ribosome synthesis are potential contributors to muscle wasting. MicroRNAs (miRNAs) are known regulators of muscle phenotype and have been shown to modulate components of the protein synthetic pathway...
December 7, 2017: Journal of Cachexia, Sarcopenia and Muscle
https://www.readbyqxmd.com/read/29214976/stray-cats-are-more-frequently-infected-with-zoonotic-protists-than-pet-cats
#15
Martin Kvac, Lada Hofmannova, Ynes Ortega, Nikola Holubova, Michaela Horcickova, Marta Kicia, Lenka Hlaskova, Dana Kvetonova, Bohumil Sak, John McEvoy
Faecal samples were collected from cats kept as pets (n = 120) and stray cats (n = 135) in Central Europe (Czech Republic, Poland and Slovakia) and screened for the presence of Cryptosporidium spp., Giardia intestinalis (Kunstler, 1882), Encephalitozoon spp. and Enterocytozoon bieneusi Desportes, Le Charpentier, Galian, Bernard, Cochand-Priollet, Lavergne, Ravisse et Modigliani, 1985 by PCR analysis of the small-subunit of rRNA (Cryptosporidium spp. and G. intestinalis) and ITS (microsporidia) genes. Sequence analysis of targeted genes revealed the presence of C...
December 6, 2017: Folia Parasitologica
https://www.readbyqxmd.com/read/29214754/utility-of-conventional-culture-and-maldi-tof-ms-for-identification-of-microbial-communities-in-bronchoalveolar-lavage-fluid-in-comparison-with-the-gs-junior-next-generation-sequencing-system
#16
Ji Yeon Sung, Younjee Hwang, Mi Hwa Shin, Moo Suk Park, Sang Hoon Lee, Dongeun Yong, Kyungwon Lee
BACKGROUND: Diverse microbiota exist in the lower respiratory tract. Although next generation sequencing (NGS) is the most widely used microbiome analysis technique, it is difficult to implement NGS in clinical microbiology laboratories. Therefore, we evaluated the performance of conventional culture methods together with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) in identifying microbiota in bronchoalveolar lavage (BAL) fluid. METHODS: BAL fluid samples (n=27) were obtained from patients undergoing diagnostic bronchoscopy for lung mass evaluation...
March 2018: Annals of Laboratory Medicine
https://www.readbyqxmd.com/read/29214493/virgibacillus-kimchii-sp-nov-a-halophilic-bacterium-isolated-from-kimchi
#17
Young Joon Oh, Ja-Young Jang, Seul Ki Lim, Min-Sung Kwon, Jieun Lee, NamHee Kim, Mi-Young Shin, Hyo Kyeong Park, Myung-Ji Seo, Hak-Jong Choi
A Gram-stain-positive, halophilic, rod-shaped, non-motile, spore forming bacterium, strain NKC1-2T, was isolated from kimchi, a Korean fermented food. Comparative analysis based on 16S rRNA gene sequence demonstrated that the isolated strain was a species of the genus Virgibacillus. Strain NKC1-2T exhibited high level of 16S rRNA gene sequence similarity with the type strains of Virgibacillus xinjiangensis SL6-1T (96.9%), V. sediminis YIM kkny3T (96.8%), and V. salarius SA-Vb1T (96.7%). The isolate grew at pH 6...
December 2017: Journal of Microbiology / the Microbiological Society of Korea
https://www.readbyqxmd.com/read/29214491/cecal-microbiome-divergence-of-broiler-chickens-by-sex-and-body-weight
#18
Kyu-Chan Lee, Dong Yong Kil, Woo Jun Sul
The divergence of gut bacterial community on broiler chickens has been reported as potentially possible keys to enhancing nutrient absorption, immune systems, and increasing poultry health and performance. Thus, we compared cecal bacterial communities and functional predictions by sex and body weight regarding the association between cecal microbiota and chicken growth performance. In this study, a total of 12 male and 12 female 1-day-old broiler chickens were raised for 35 days in 2 separate cages. Chickens were divided into 3 subgroups depending on body weight (low, medium, and high) by each sex...
December 2017: Journal of Microbiology / the Microbiological Society of Korea
https://www.readbyqxmd.com/read/29214490/spirosoma-migulaei-sp-nov-isolated-from-soil
#19
Joseph Okiria, Leonid N Ten, Su-Jin Park, Seung-Yeol Lee, Dong Hoon Lee, In-Kyu Kang, Dae Sung Lee, Hee-Young Jung
A Gram-stain-negative, non-motile, non-spore-forming, rod-shaped, aerobic bacterium, designated 15J9-8T, was isolated from soil on Jeju Island, Republic of Korea. The isolate was able to grow between 10 and 30°C, pH 6.5-8.5, and in presence of 0-1% (w/v) NaCl. The results of comparative 16S rRNA gene sequence analysis indicated that strain 15J9-8T represented a member of the family Cytophagaceae, phylum Bacteroidetes, and was most closely related to Spirosoma aerophilum 5516J-17T (96.1% similarity), Spirosoma pulveris JSH5-14T (95...
December 2017: Journal of Microbiology / the Microbiological Society of Korea
https://www.readbyqxmd.com/read/29214486/metabolism-mediated-induction-of-zinc-tolerance-in-brassica-rapa-by-burkholderia-cepacia-cs2-1
#20
Sang-Mo Kang, Raheem Shahzad, Saqib Bilal, Abdul Latif Khan, Young-Hyun You, Won-Hee Lee, Hee-La Ryu, Ko-Eun Lee, In-Jung Lee
Brassica rapa (Chinese cabbage) is an essential component of traditional Korean food. However, the crop is often subject to zinc (Zn+) toxicity from contaminated irrigation water, which, as a result, compromises plant growth and production, as well as the health of human consumers. The present study investigated the bioaccumulation of Zn+ by Burkholderia cepacia CS2-1 and its effect on the heavy metal tolerance of Chinese cabbage. Strain CS2-1 was identified and characterized on the basis of 16S rRNA sequences and phylogenetic analysis...
December 2017: Journal of Microbiology / the Microbiological Society of Korea
keyword
keyword
28858
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"