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Narjes Dashti, Nedaa Ali, Samar Salamah, Majida Khanafer, Ghada Al-Shamy, Husain Al-Awadhi, Samir S Radwan
To analyze microbial communities in environmental samples, this study combined Denaturing Gradient Gel Electrophoresis of amplified 16S rRNA-genes in total genomic DNA extracts from those samples with gene sequencing. The environmental samples studied were oily seawater and soil samples, that had been bioaugmented with natural materials rich in hydrocarbonoclastic bacteria. This molecular approach revealed much more diverse bacterial taxa than the culture-dependent method we had used in an earlier study for the analysis of the same samples...
April 15, 2018: MicrobiologyOpen
Dattatray K Bedade, Rekha S Singhal
Acrylamide is neurotoxic, genotoxic, teratogenic and carcinogenic. Its widespread use in various industrial processes leads to environmental contamination. Acrylamidase produced by certain bacteria degrade acrylamide to acrylic acid and ammonia. The present study details the isolation and identification of soil bacterium which could degrade acrylamide. Among the 18 acrylamide-degrading isolates tested, isolate ICTDB921 demonstrated superior acrylamide degradation which was confirmed by HPLC, FTIR and GC-MS...
April 6, 2018: Bioresource Technology
Raj Kumar Pandey, Salil Tewari, Lakshmi Tewari
A mycoremedial study was undertaken for decolourization of synthetic dyes using wood rot fungal culture Lenzites elegans WDP2. The culture was isolated from decaying wood as fruiting body, and identified on the basis of 5.8S ITS rRNA gene sequence analysis. Qualitative plate screening of culture showed extracellular laccase and lignin peroxidase production, while only laccase enzyme was produced in higher amount (156.793 Uml-1 ) in minimal salt broth medium containing glucose and veratryl alcohol. Laccase activity was increased up to 189...
April 12, 2018: Ecotoxicology and Environmental Safety
Kyung Hyun Kim, Ga Youn Cho, Byung Hee Chun, Stefan Weckx, Ji Young Moon, Soo-Hwan Yeo, Che Ok Jeon
Twelve Acetobacter pasteurianus-related strains with publicly available genomes in GenBank shared high 16S rRNA gene sequence similarity (>99.59%), but average nucleotide identity (ANI) and in silico DNA-DNA hybridization (DDH) values and multilocus sequence- and genome-based relatedness analyses suggested that they were divided into four different phylogenetic lineages. Relatedness analyses based on multilocus sequences, 1,194 core genes and whole-cell MALDI-TOF profiles supported that strains LMG 1590T and LMG 1591 (previously classified as the type strains of A...
April 5, 2018: Systematic and Applied Microbiology
Rewaida Abdel-Gaber, Reem Alajmi, Saleh Al Quraishy, Kareem Morsy, Khaled Al Rasheid
Proteocephalids are cestoda parasites that mostly infect freshwater fish. The present study was carried out to investigate the presence of proteocephalids infecting the electric catfish Malapterurus electricus from Lake Manzala, Kafr El-Sheikh Governorate, Egypt. Morphological characterization revealed the present parasite is a cestoda belonging to the genus Electrotaenia. Morphologically, the recovered worms were characterized by an elongated body measuring 100-127 (120 ± 2) mm long and 0.92-2.11 (2...
April 12, 2018: Acta Tropica
Min-Goo Seo, In-Ohk Ouh, Oh-Deog Kwon, Dongmi Kwak
Anaplasma phagocytophilum is the causative agent of human granulocytic anaplasmosis and tick-borne fever in domestic ruminants. Differential diagnosis of zoonotic and pathogenic tick-borne diseases like granulocytic anaplasmosis is important for the efficient implementation of control programs. Thus, the differentiation of pathogenic A. phagocytophilum from non-pathogenic A. phagocytophilum-like (APL) Anaplasma spp. is essential. Recent molecular analyses of APL revealed its distinct phylogenetic position from A...
April 10, 2018: Molecular Phylogenetics and Evolution
Yongguo Cao, Xufeng Xie, Wenlong Zhang, Dianjun Wu, Changchun Tu
Currently, accumulating evidence is challenging subtherapeutic therapy. Low-dose Norfloxacin (Nor) has been reported to suppress the immune response and worsen leptospirosis. In this study, we investigated the influence of low-dose Nor (0.03 μg/ml, 0.06 μg/ml, 0.125 μg/ml) on leptospiral gene expression and analyzed the immunomodulatory effects of low-dose Nor-treated leptospires in J774A.1 cells. To study the expression profiles of low-dose Nor-treated leptospires, we chose LipL71/LipL21 as reference genes determined by the geNorm applet in this experiment...
April 10, 2018: Microbial Pathogenesis
Waheda Rahman Ansary, Ferdous Rezwan Khan Prince, Effi Haque, Farzana Sultana, Helen M West, Mahbubur Rahman, Abdul Mojid Mondol, Abdul Mannan Akanda, Mahfuz Rahman, Michele L Clarke, Tofazzal Islam
Plant growth-promoting bacteria that are also capable of suppressing plant pathogenic fungi play an important role in sustainable agriculture. There is a critical need for conducting research to discover, characterize and evaluate the efficacy of new strains of such bacteria in controlling highly aggressive plant pathogens. In this study, we isolated endophytic bacteria from medicinal plants of Bangladesh and evaluated their antagonistic capacity against an important phytopathogenic fungus Sclerotinia sclerotiorum...
April 11, 2018: Zeitschrift Für Naturforschung. C, A Journal of Biosciences
Qian Yang, Jinyao Li, Jing Shen, Yufang Xu, Hongjie Liu, Wei Deng, Xuefeng Li, Mingqi Zheng
D. sophia is one of the most notorious broadleaf weed in China, and has evolved extremely high resistance to ALS-inhibiting herbicide tribenuron-methyl. The target-site resistance due to ALS gene mutations was known well, while the non-target-site resistance is not yet well-characterized. Metabolic resistance, which is conferred by enhanced rates of herbicide metabolism, is the most important NTSR. To explore the mechanism of metabolic resistance underlying resistant (R) D. sophia plants, tribenuron-methyl uptake and metabolism levels, qPCR reference gene stability, and candidate P450 genes expression patterns were investigated...
April 13, 2018: Journal of Agricultural and Food Chemistry
Alanna Dubrovsky, Christopher L Kitts
A 20-year-old female was diagnosed with ulcerative colitis (UC) at age 14 and primary sclerosing cholangitis (PSC) at age 16. The PSC was successfully treated with high doses of oral vancomycin; however, the UC was more difficult to manage. After many drug treatments failed to treat the UC, the patient began following the specific carbohydrate diet (SCD). This report documents fecal microbiome changes resulting from following the SCD for two weeks. The DNA extracted from fecal samples was subjected to 16S rRNA gene sequencing to quantify bacterial species abundance...
February 9, 2018: Curēus
Eun-Jeong Yoon, Da Young Kang, Ji Woo Yang, Dokyun Kim, Hyukmin Lee, Kwang Jun Lee, Seok Hoon Jeong
This study was carried out to investigate the epidemiological time-course of New Delhi metallo-beta-lactamase- (NDM-) mediated carbapenem resistance in Enterobacteriaceae in South Korea. A total of 146 non-duplicate NDM-producing Enterobacteriaceae recovered between 2010 and 2015 were voluntarily collected from 33 general hospitals and confirmed by PCR. The species were identified by sequences of the 16S rDNA. Antimicrobial susceptibility was determined either by the disk diffusion method or by broth microdilution, and the carbapenem MICs were determined by agar dilution...
2018: Frontiers in Microbiology
Xiaoming Tan, Yaqin Zhou, Xiaolei Zhou, Xianghua Xia, Ying Wei, Lili He, Hongzhen Tang, Liyin Yu
The plant Dysosma versipellis is known for its antimicrobial and anticancer properties but is a rare and vulnerable perennial herb that is endemic to China. In this study, 224 isolates were isolated from various tissues of D. versipellis, and were classified into 53 different morphotypes according to culture characteristics and were identified by sequence analyses of the internal transcribed spacer (ITS) region of the rRNA gene. Although nine strains were not assignable at the phylum level, 44 belonged to at least 29 genera of 15 orders of Ascomycota (93%), Basidiomycota (6%), and Zygomycota (1%)...
April 12, 2018: Scientific Reports
Emil Hovius, Arnout de Bruin, Leo Schouls, Joppe Hovius, Niels Dekker, Hein Sprong
BACKGROUND: Various tick-borne infections often occur without specific clinical signs and are therefore notoriously hard to diagnose separately in veterinary practice. Longitudinal studies over multiple tick seasons performing clinical, serological and molecular investigations in parallel, may elucidate the relationship between infection and disease. In this regard, six related Rhodesian Ridgeback dogs living as a pack became subject of lifetime studies due to ongoing tick infestations and recurring clinical problems...
April 12, 2018: Parasites & Vectors
Jianying Huang, Zhenjie Zhang, Yiqi Zhang, Yong Yang, Jinfeng Zhao, Rongjun Wang, Fuchun Jian, Changshen Ning, Wanyu Zhang, Longxian Zhang
BACKGROUND: Little is known about the prevalence and zoonotic potential of Cryptosporidium spp. and Giardia duodenalis in deer in China. In this study, 662 fecal samples were collected from 11 farms in Henan and Jilin Provinces between July 2013 and August 2014, and were screened for the presence of Cryptosporidium and G. duodenalis with genotyping and subtyping methods. RESULTS: Cryptosporidium spp. and G. duodenalis were detected in 6.80% (45/662) and 1.21% (5/662) of samples, respectively...
April 12, 2018: Parasites & Vectors
Bin Ji, Hui Qin, Shaodong Guo, Wei Chen, Xuechun Zhang, Jiechao Liang
Knowing the microbial compositions in fresh lakes is significant to explore the mechanisms of eutrophication and algal blooms. This study reported on the bacterial communities of the four adjacent fresh lakes at different trophic status by Illumina MiSeq Platform, which were Tangxun Lake (J1), Qingling Lake (J2), Huangjia Lake (J3) and Niushan Lake (J4) in Wuhan, China. J1 had the highest salinity and phosphorus. J2 was abundant in TC (Total Carbon)/TOC (Total Organic Carbon.), calcium and magnesium. J3 had the highest content of nitrogen, iron and pollution of heavy metals...
April 9, 2018: Ecotoxicology and Environmental Safety
Tian Jiang, Jing Zhang, Liwei Rong, Yanjiang Feng, Qi Wang, Qiulai Song, Lixin Zhang, Min Ouyang
Chloroplast development is a highly complex process and the regulatory mechanism has not been fully characterized. Identification of the corresponding regulators will shed light on the intricacies of chloroplast development. Here, we identified ECD1 (Early Chloroplast Development 1), a chloroplast-localized pentatricopeptide repeat protein (PPR) belonging to the PLS subfamily. Inactivation of ECD1 leads to embryo lethality, and abnormal embryogenesis occurs in ecd1/+ heterozygous plants. A decrease of ECD1 induced by RNA interference (RNAi) results in seedlings with albino cotyledons but normal true leaves...
April 10, 2018: Journal of Experimental Botany
Quinn S McFrederick, Hoang Q Vuong, Jason A Rothman
Gram-stain-positive, rod-shaped, non-spore forming bacteria have been isolated from flowers and the guts of adult wild bees in the families Megachilidae and Halictidae. Phylogenetic analysis of the 16S rRNA gene indicated that these bacteria belong to the genus Lactobacillus, and are most closely related to the honey-bee associated bacteria Lactobacillus kunkeei (97.0 % sequence similarity) and Lactobacillus apinorum (97.0 % sequence similarity). Phylogenetic analyses of 16S rRNA genes and six single-copy protein coding genes, in situ and in silico DNA-DNA hybridization, and fatty-acid profiling differentiates the newly isolated bacteria as three novel Lactobacillus species: Lactobacillus micheneri sp...
April 12, 2018: International Journal of Systematic and Evolutionary Microbiology
Ping Mo, Jiarong Zhao, Kaiqin Li, Xinke Tang, Jian Gao
A novel actinomycete isolate, designated strain MK44T , was isolated from a Manganese-polluted soil sample collected near Xiangtan Manganese Mine, South Central China and subjected to a polyphasic taxonomic characterization. Comparison of 16S rRNA gene sequences showed that strain MK44T was a member of the genus Streptomyces and most closely related to Streptomyces specialis JCM 16611T (97.9 %) and Streptomyces mayteni JCM 16957T (97.4 %). The DNA-DNA relatedness between strain MK44T and the above two related type species were 30...
April 12, 2018: International Journal of Systematic and Evolutionary Microbiology
Ram Hari Dahal, Jaisoo Kim
A novel bacterium, designated strain CA-1T , was isolated from forest soil in Kyonggi University. Cells were strictly aerobic, Gram-stain-negative, catalase-positive, oxidase-negative, non-motile, non-spore-forming, rod-shaped and red-orange-pigmented. Strain CA-1T hydrolysed casein and DNA. It was able to grow at 15-37 °C, pH 5.5-9.0 and at 0-2 % (w/v) NaCl concentration. Flexirubin-type pigments were present. Phylogenetic analysis based on its 16S rRNA gene sequence indicated that strain CA-1T formed a lineage within the family Crocinitomicaceae of the phylum Bacteroidetes that was distinct from Fluviicola hefeinensis MYL-8T (96...
April 12, 2018: International Journal of Systematic and Evolutionary Microbiology
Jing Sun, Shiquan Qian, Jing Lu, Yanan Liu, Fengxia Lu, Xiaomei Bie, Zhaoxin Lu
Bacillus amyloliquefaciens, a gram-positive and soil-dwelling bacterium, could produce secondary metabolites that suppress plant pathogens. In this study, we provided the whole genome sequence results of B. amyloliquefaciens fmbJ which had one circular chromosome of 4,193,344 bp with 4,249 genes, 87 transfer RNA genes, and 27 rRNA genes. In addition, fmbJ was found to contain several gene clusters of antimicrobial lipopeptides (bacillomycin D, surfactin, and fengycin) and bacillomycin D homologues were further comprehensively identified...
April 12, 2018: Journal of Agricultural and Food Chemistry
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