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https://www.readbyqxmd.com/read/28654217/dual-labelled-antibodies-for-flow-and-mass-cytometry-a-new-tool-for-cross-platform-comparison-and-enrichment-of-target-cells-for-mass-cytometry
#1
EDITORIAL
Sabine Baumgart, Axel R Schulz, Anette Peddinghaus, Silke Stanislawiak, Sarah Gillert, Heike Hirseland, Susanne Krauthäuser, Christian Dose, Henrik E Mei, Andreas Grützkau
Antibody conjugates applicable in both conventional flow and mass cytometry would offer interesting options for cross-platform comparison, as well as the enrichment of rare target cells by conventional flow cytometry (FC) sorting prior to deep phenotyping by mass cytometry (MC). Here, we introduce a simple method to generate dual fluorochrome/metal-labelled antibodies by consecutive orthogonal labelling. First we compared different fluorochrome-conjugated antibodies specific for CD4, such as FITC, Vio667, VioGreen or VioBlue for their compatibility with the conventional secondary MAXPAR(®) labelling protocol...
June 27, 2017: European Journal of Immunology
https://www.readbyqxmd.com/read/28652580/immunological-phenotype-of-the-murine-lrba-knockout
#2
Laura Gámez-Díaz, Julika Neumann, Fiona Jäger, Michele Proietti, Felicitas Felber, Pauline Soulas-Sprauel, Lisa Perruzza, Fabio Grassi, Tamara Kögl, Peter Aichele, Manfred Kilimann, Bodo Grimbacher, Sophie Jung
Biallelic mutations in the human Lipopolysaccharide Responsive Beige-like Anchor (LRBA) gene lead to a primary immunodeficiency known as LRBA deficiency, characterized by a broad range of clinical manifestations including autoimmunity, organomegaly, hypogammaglobulinemia and recurrent infections. Considering the phenotypic heterogeneity in patients and the severity of the disease, our aim was to assess the role of LRBA in immune cells and to understand the underlying pathomechanisms through the study of a Lrba knockout (Lrba(-/-)) mouse model...
June 27, 2017: Immunology and Cell Biology
https://www.readbyqxmd.com/read/28633925/the-discovery-of-plasma-cells-an-historical-note
#3
REVIEW
Domenico Ribatti
The name plasma cell was introduced by the anatomist Heinrich H. von Hartz-Waldeyer in 1875. Plasma cells derive from small B lymphocytes after their activation. A fully mature plasma cell lacks surface immunoglobulin expression. Its form is round or oval, with characteristic basophilic cytoplasm and an eccentric nucleus that contains coarse heterochromatin. Antigen activation of mature B cells leads initially to germinal center development, the transient generation of plasmablasts that secrete antibody while still dividing, and short-lived extrafollicular plasma cells that secrete antigen-specific germ line-encoded antibodies...
June 17, 2017: Immunology Letters
https://www.readbyqxmd.com/read/28628673/multiple-tolerance-defects-contribute-to-the-breach-of-b-cell-tolerance-in-new-zealand-black-chromosome-1-congenic-mice
#4
Nan-Hua Chang, Kieran P Manion, Christina Loh, Evelyn Pau, Yuriy Baglaenko, Joan E Wither
Lupus is characterized by a loss of B cell tolerance leading to autoantibody production. In this study, we explored the mechanisms underlying this loss of tolerance using B6 congenic mice with an interval from New Zealand Black chromosome 1 (denoted c1(96-100)) sufficient for anti-nuclear antibody production. Transgenes for soluble hen egg white lysozyme (sHEL) and anti-HEL immunoglobulin were crossed onto this background and various tolerance mechanisms examined. We found that c1(96-100) mice produced increased levels of IgM and IgG anti-HEL antibodies compared to B6 mice and had higher proportions of germinal center B cells and long-lived plasma cells, suggesting a germinal center-dependent breach of B cell anergy...
2017: PloS One
https://www.readbyqxmd.com/read/28626065/metabolic-reprogramming-commits-differentiation-of-human-cd27-igd-b-cells-to-plasmablasts-or-cd27-igd-cells
#5
Masataka Torigoe, Shigeru Iwata, Shingo Nakayamada, Kei Sakata, Mingzeng Zhang, Maiko Hajime, Yusuke Miyazaki, Manabu Narisawa, Koji Ishii, Hirotaka Shibata, Yoshiya Tanaka
B cells play a crucial role in the pathogenesis of autoimmune diseases, such as systemic lupus erythematosus (SLE). However, the relevance of the metabolic pathway in the differentiation of human B cell subsets remains unknown. In this article, we show that the combination of CpG/TLR9 and IFN-α markedly induced the differentiation of CD27(+)IgD(+) unswitched memory B cells into CD27(hi)CD38(hi) plasmablasts. The response was accompanied by mammalian target of rapamycin complex 1 (mTORC1) activation and increased lactate production, indicating a shift to glycolysis...
June 16, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/28625807/evidence-for-altered-levels-of-immunoglobulin-d-in-the-nasal-airway-mucosa-of-patients-with-chronic-rhinosinusitis
#6
Jin-Young Min, Jayakar V Nayak, Kathryn E Hulse, Whitney W Stevens, Paul A Raju, Julia H Huang, Lydia A Suh, Griet A Van Roey, James E Norton, Roderick G Carter, Caroline P E Price, Ava R Weibman, Ali R Rashan, Eliver E Ghosn, Zara M Patel, Tetsuya Homma, David B Conley, Kevin C Welch, Stephanie Shintani-Smith, Anju T Peters, Leslie C Grammer, Kathleen E Harris, Atsushi Kato, Peter H Hwang, Robert C Kern, Leonore A Herzenberg, Robert P Schleimer, Bruce K Tan
BACKGROUND: Immunoglobulin D (IgD) is an enigmatic antibody isotype best known when co-expressed with IgM on naïve B cells. However, elevated soluble IgD (sIgD) and increased IgD(+)IgM(-) B cell populations have been described in the human upper respiratory mucosa. OBJECTIVE: We assessed whether levels of sIgD and IgD(+) B cells are altered in nasal tissue from patients with chronic rhinosinusitis (CRS). We further characterized IgD(+) B cell populations and explored clinical and local inflammatory factors associated with tissue sIgD levels...
June 15, 2017: Journal of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/28622453/plasmablasts-with-a-mucosal-phenotype-contribute-to-plasmacytosis-in-sle
#7
Henrik E Mei, Stefanie Hahne, Andreas Redlin, Bimba F Hoyer, Kaiyin Wu, Lisa Baganz, Anna R Lisney, Tobias Alexander, Birgit Rudolph, Thomas Dörner
Objective To analyze the composition of known plasmacytosis in SLE to obtain further insight into the nature of underlying mechanisms. Methods Plasmablasts of active and inactive/treated SLE patients, and of healthy controls were characterized by flow cytometry, EliSpot and transwell migration assays, and results were compared to characteristics of vaccination-induced plasmablasts. Serum cytokine levels were analyzed by a Luminex assay, and kidney biopsies by histology. Results We here demonstrate the expression of markers of mucosal immune reactions by circulating plasmablasts in SLE...
June 16, 2017: Arthritis & Rheumatology
https://www.readbyqxmd.com/read/28620653/successive-annual-influenza-vaccination-induces-a-recurrent-oligoclonotypic-memory-response-in-circulating-t-follicular-helper-cells
#8
Ramin Sedaghat Herati, Alexander Muselman, Laura Vella, Bertram Bengsch, Kaela Parkhouse, Daniel Del Alcazar, Jonathan Kotzin, Susan A Doyle, Pablo Tebas, Scott E Hensley, Laura F Su, Kenneth E Schmader, E John Wherry
T follicular helper (Tfh) CD4 cells are crucial providers of B cell help during adaptive immune responses. A circulating population of CD4 T cells, termed cTfh, have similarity to lymphoid Tfh, can provide B cell help, and responded to influenza vaccination. However, it is unclear whether human vaccination-induced cTfh respond in an antigen-specific manner and whether they form long-lasting memory. Here, we identified a cTfh population that expressed multiple T cell activation markers and could be readily identified by coexpression of ICOS and CD38...
February 2017: Science Immunology
https://www.readbyqxmd.com/read/28620390/belatacept-does-not-inhibit-follicular-t-cell-dependent-b-cell-differentiation-in-kidney-transplantation
#9
Gretchen N de Graav, Dennis A Hesselink, Marjolein Dieterich, Rens Kraaijeveld, Wenda Verschoor, Dave L Roelen, Nicolle H R Litjens, Anita S Chong, Willem Weimar, Carla C Baan
Humoral alloreactivity has been recognized as a common cause of kidney transplant dysfunction. B-cell activation, differentiation, and antibody production are dependent on IL-21(+)CXCR5(+)follicular T-helper (Tfh) cells. Here, we studied whether belatacept, an inhibitor of the costimulatory CD28-CD80/86-pathway, interrupts the crosstalk between Tfh- and B-cells more efficiently than the calcineurin inhibitor tacrolimus. The suppressive effects of belatacept and tacrolimus on donor antigen-driven Tfh-B-cell interaction were functionally studied in peripheral blood mononuclear cells from 40 kidney transplant patients randomized to a belatacept- or tacrolimus-based immunosuppressive regimen...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28617827/nog-hil-4-tg-a-new-humanized-mouse-model-for-producing-tumor-antigen-specific-igg-antibody-by-peptide-vaccination
#10
Yoshie Kametani, Ikumi Katano, Asuka Miyamoto, Yusuke Kikuchi, Ryoji Ito, Yukari Muguruma, Banri Tsuda, Sonoko Habu, Yutaka Tokuda, Kiyoshi Ando, Mamoru Ito
Immunodeficient mice transplanted with human peripheral blood mononuclear cells (PBMCs) are promising tools to evaluate human immune responses to vaccines. However, these mice usually develop severe graft-versus-host disease (GVHD), which makes estimation of antigen-specific IgG production after antigen immunization difficult. To evaluate antigen-specific IgG responses in PBMC-transplanted immunodeficient mice, we developed a novel NOD/Shi-scid-IL2rγnull (NOG) mouse strain that systemically expresses the human IL-4 gene (NOG-hIL-4-Tg)...
2017: PloS One
https://www.readbyqxmd.com/read/28614800/robust-memory-responses-against-influenza-vaccination-in-pemphigus-patients-previously-treated-with-rituximab
#11
Alice Cho, Bridget Bradley, Robert Kauffman, Lalita Priyamvada, Yevgeniy Kovalenkov, Ron Feldman, Jens Wrammert
Rituximab is a therapeutic anti-CD20 monoclonal antibody widely used to treat B cell lymphoma and autoimmune diseases, such as rheumatic arthritis, systemic lupus erythematosus, and autoimmune blistering skin diseases (AIBD). While rituximab fully depletes peripheral blood B cells, it remains unclear whether some preexisting B cell memory to pathogens or vaccines may survive depletion, especially in lymphoid tissues, and if these memory B cells can undergo homeostatic expansion during recovery from depletion...
June 15, 2017: JCI Insight
https://www.readbyqxmd.com/read/28608720/diagnostic-algorithm-of-common-mature-b-cell-lymphomas-by-immunohistochemistry
#12
Huan-You Wang, Youli Zu
CONTEXT: - Different types of mature B-cell lymphomas, including plasma cell neoplasms, exhibit distinct immunohistochemical profiles, which enable them to be correctly diagnosed. However, except for rare examples of lymphoma-specific immunohistochemistry, such as cyclin D1 in mantle cell lymphoma and annexin A1 in hairy cell leukemia, immunohistochemical profiles of mature B-cell lymphomas overlap and lack specificity. OBJECTIVES: - To systemically review immunohistochemical features associated with commonly encountered, mature B-cell lymphomas based on the presence or absence of CD5 and CD10; to review the immunophenotypic profile of plasma cells derived from plasma cell myelomas and B-cell lymphomas; and to review a group of rare, aggressive B-cell lymphomas with antigen expression features of plasma cells...
June 13, 2017: Archives of Pathology & Laboratory Medicine
https://www.readbyqxmd.com/read/28608550/a-new-staining-protocol-for-detection-of-murine-antibody-secreting-plasma-cell-subsets-by-flow-cytometry
#13
Katharina Pracht, Julia Meinzinger, Patrick Daum, Sebastian R Schulz, Dorothea Reimer, Manuela Hauke, Edith Roth, Dirk Mielenz, Claudia Berek, Joana Côrte-Real, Hans-Martin Jäck, Wolfgang Schuh
In mice, CD138, B220 and Blimp1, a plasma cell (PC) signature transcription factor, are frequently used to quantify mature antibody-secreting PCs. Here, we show in a Blimp1:GFP reporter mouse that the CD138(hi) /B220(low) population in bone marrow (BM) contains a significant fraction of Blimp1-negative cells. Therefore, detection of B220 and CD138 alone is not sufficient to identify PCs unambiguously. To circumvent this problem, we developed a new 4-color PC staining protocol using antibodies against CD138, B220, transmembrane activator and CAML interactor (TACI) and CD19...
June 13, 2017: European Journal of Immunology
https://www.readbyqxmd.com/read/28608188/-hiv-associated-plasmablastic-lymphoma-of-the-paranasal-sinuses-an-incidental-finding
#14
V Weber, K Helling, C Matthias
A 48-year-old female patient presented to the ENT department of the University Medical Center of the Johannes Gutenberg University Mainz with subfebrile temperatures, one-sided nasal obstruction, and left-sided cephalgia. Clinical examination and CT scans showed a mass occupying the left nasal cavity and left paranasal sinuses. Further diagnosis and histopathological examination showed an HIV-associated plasmablastic lymphoma of the left paranasal sinuses. This case report with literature review discusses the diagnosis and treatment of this rare nasal tumor...
June 12, 2017: HNO
https://www.readbyqxmd.com/read/28605137/peripheral-immunophenotyping-identifies-three-subgroups-based-on-t-cell-heterogeneity-in-lupus-patients
#15
Satoshi Kubo, Shingo Nakayamada, Maiko Yoshikawa, Yusuke Miyazaki, Kei Sakata, Kazuhisa Nakano, Kentaro Hanami, Shigeru Iwata, Ippei Miyagawa, Kazuyoshi Saito, Yoshiya Tanaka
OBJECTIVE: To elucidate the diversity of systemic lupus erythematosus (SLE), we stratified active SLE patients based on immunophenotyping. METHODS: Peripheral blood mononuclear cells were obtained from 143 SLE patients and 49 healthy individuals. Circulating B, T and dendritic cells were defined based on flow cytometric analysis for human immune system termed "the Human Immunology Project". Based on these results, the immunophenotype was visualized by principal component analysis and SLE patients classified into subgroups by cluster analysis...
June 12, 2017: Arthritis & Rheumatology
https://www.readbyqxmd.com/read/28604797/a-human-inferred-germline-antibody-binds-to-an-immunodominant-epitope-and-neutralizes-zika-virus
#16
Diogo M Magnani, Cassia G T Silveira, Brandon C Rosen, Michael J Ricciardi, Núria Pedreño-Lopez, Martin J Gutman, Varian K Bailey, Helen S Maxwell, Aline Domingues, Lucas Gonzalez-Nieto, Vivian I Avelino-Silva, Mateus Trindade, Juliana Nogueira, Consuelo S Oliveira, Alvino Maestri, Alvina Clara Felix, José Eduardo Levi, Mauricio L Nogueira, Mauricio A Martins, José M Martinez-Navio, Sebastian P Fuchs, Stephen S Whitehead, Dennis R Burton, Ronald C Desrosiers, Esper G Kallas, David I Watkins
The isolation of neutralizing monoclonal antibodies (nmAbs) against the Zika virus (ZIKV) might lead to novel preventative strategies for infections in at-risk individuals, primarily pregnant women. Here we describe the characterization of human mAbs from the plasmablasts of an acutely infected patient. One of the 18 mAbs had the unusual feature of binding to and neutralizing ZIKV despite not appearing to have been diversified by affinity maturation. This mAb neutralized ZIKV (Neut50 ~ 2 μg/ml) but did not react with any of the four dengue virus serotypes...
June 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28592757/hiv-negative-plasmablastic-lymphoma-attaining-sustained-remission-with-bortezomib-combined-dose-adjusted-epoch-therapy
#17
Hiroyuki Kobayashi, Norito Miyagi
A 61-year-old, HIV-negative, immunocompetent woman was admitted to our hospital for significant weight loss, painful swelling of her right cheek, and rapid growth of a tumor in the right hard palate. A CT scan revealed a neoplastic lesion in the right maxillary sinus, extending to the surrounding soft bone tissue and oral cavity, as well as multiple osteolytic lesions in the skull. A pathologic examination revealed that the neoplastic cells in the oral cavity were plasmablasts tending to appear as differentiated plasma cells...
2017: [Rinshō Ketsueki] the Japanese Journal of Clinical Hematology
https://www.readbyqxmd.com/read/28589344/detecting-gene-expression-in-lymphoid-microenvironments-by-laser-microdissection-and-quantitative-rt-pcr
#18
Yang Zhang, Laura Garcia-Ibanez, Geoffrey Brown, Kai-Michael Toellner
Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) is a valuable tool for measuring gene expression in cells and tissues. Unique challenges are encountered when studies are performed on cells microdissected from small specific areas of frozen animal or human tissue. This chapter describes the analysis of gene expression of chemokines and cytokines that are important for the differentiation and migration of germinal center (GC) derived plasmablasts/plasma cells and memory B cells by using laser capture microdissection (LCM) and qRT-PCR to examine tissue sections...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28589164/cns-aquaporin-4-specific-b-cells-connect-with-multiple-b-cell-compartments-in-neuromyelitis-optica-spectrum-disorder
#19
Markus C Kowarik, David Astling, Christiane Gasperi, Scott Wemlinger, Hannah Schumann, Monika Dzieciatkowska, Alanna M Ritchie, Bernhard Hemmer, Gregory P Owens, Jeffrey L Bennett
OBJECTIVES: Neuromyelitis optica spectrum disorder (NMOSD) is a severe inflammatory disorder of the central nervous system (CNS) targeted against aquaporin-4 (AQP4). The origin and trafficking of AQP4-specific B cells in NMOSD remains unknown. METHODS: Peripheral (n = 7) and splenic B cells (n = 1) recovered from seven NMOSD patients were sorted into plasmablasts, naïve, memory, and CD27-IgD- double negative (DN) B cells, and variable heavy chain (VH) transcriptome sequences were generated by deep sequencing...
June 2017: Annals of Clinical and Translational Neurology
https://www.readbyqxmd.com/read/28588836/a-case-of-hiv-negative-plasmablastic-lymphoma-of-the-bone-marrow-with-a-unique-immunophenotype
#20
Christopher Dittus, Shayna Sarosiek
We present an interesting case of plasmablastic lymphoma, which is a rare type of non-Hodgkin lymphoma that is typically diagnosed in HIV-positive patients and has an immunophenotype that overlaps with multiple myeloma. Our patient is unique because he is HIV-negative, has primary bone marrow disease, and has an atypical immunophenotype.
June 2017: Clinical Case Reports
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