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Y-X Cao, Z-Q Wang, J-X Kang, K Liu, C-F Zhao, Y-X Guo, B-H Jiao
OBJECTIVE: It's of great significance to investigate the novel targets of drugs for the treatment of stroke. In this study, we explored the neuroprotective role of miR-424 in oxygen glucose deprivation (OGD)-induced injuries in PC-12 cells. MATERIALS AND METHODS: PC-12 cells were subjected to OGD stimulation to mimic ischemic injury. The expressions of miR-424 and mitogen-activated protein kinase phosphatase-1 (MKP-1) were altered by transient transfection with miR-424 mimic, miR-424 inhibitor, pEX-MKP-1, or sh-MKP-1...
March 2018: European Review for Medical and Pharmacological Sciences
F-D Sun, P-C Wang, R-L Luan, S-H Zou, X Du
OBJECTIVE: Drug resistance has become an important factor that threatens the survival and prognosis of patients with breast cancer, especially in patients with advanced breast cancer. Several microRNAs have been proved to participate in the resistant process; however, the role of miR-574 in doxorubicin (Dox) resistant breast cancer is still unclear. PATIENTS AND METHODS: Quantitative Real-time poly chain reaction (qRT-PCR) was employed to detect the expression level of miR-574 in breast cancer Dox-resistant MCF-7/Adr cell line and parental MCF-7 cell line...
March 2018: European Review for Medical and Pharmacological Sciences
X Yang, W Zhang, S-Q Cheng, R-L Yang
OBJECTIVE: To clarify the role of long non-coding RNA (lncRNA) GACAT3 in invasion and metastasis of non-small cell lung cancer (NSCLC) and its effect on radiotherapy. PATIENTS AND METHODS: The expression of GACAT3 and TIMP2 in cells and tissues of NSCLC were detected by quantitative Real-time PCR (qRT-PCR). The influence of GACAT3 on cell proliferation and the capacity of colony formation were estimated by MTT test and colony forming experiment respectively. Luciferase reporting assay was used to confirm the correlation between GACAT3 and TIMP2...
March 2018: European Review for Medical and Pharmacological Sciences
W-W Liao, C Zhang, F-R Liu, W-J Wang
OBJECTIVE: MiR-155 has been shown to be up-regulated in hepatocellular carcinoma (HCC) patients. B-cell lymphoma-2 (Bcl-2) interacting mediator of cell death (BIM) regulates cell proliferation and apoptosis, as its down-regulation is involved in HCC onset. Transcriptional factor FoxO3a mediates BIM expression and is related to HCC pathogenesis. Bioinformatics analysis showed targeted regulation of FoxO3a by miR-155. This study aims to investigate whether miR-155 plays a role in mediating FoxO3a/BIM signal pathway and HCC occurrence...
March 2018: European Review for Medical and Pharmacological Sciences
X-G Liu, J Xu, F Li, M-J Li, T Hu
OBJECTIVE: Chemoresistance is one of the obstacles for effective treatment of cancers, and recent evidence has shown that microRNAs play critical roles in drug resistance. In this study, we investigated the effect of miR-377 on cisplatin resistance in osteosarcoma (OS). PATIENTS AND METHODS: Tumor tissues from chemoresistant and control OS patients were subjected to Real-time polymerase chain reaction (PCR) to assess miR-377 expression. The effect and mechanism of miR-377 on cisplatin resistance were assessed using Cell Counting Kit (CCK) 8, flow cytometry, Western blot, and luciferase assays in cisplatin-resistant OS cells lines...
March 2018: European Review for Medical and Pharmacological Sciences
D-W Wang, Y-Q Wang, H-S Shu
OBJECTIVE: Extracellular signal-regulated kinase (ERK)/mitogen activated protein kinase (MAPK) signal pathway participates in cell proliferation, cycle, and apoptosis. MiR-16 is down-regulated in the pituitary tumor. This study investigated the role and related mechanism of miR-16 on pituitary tumor proliferation, cycle, and apoptosis. PATIENTS AND METHODS: Dual-luciferase reporter assay was conducted to demonstrate the targeted regulation between miR-16 and MEK1...
March 2018: European Review for Medical and Pharmacological Sciences
Yiyi Yu, Ke Peng, Hong Li, Rongyuan Zhuang, Yan Wang, Wei Li, Shan Yu, Li Liang, Xiaojing Xu, Tianshu Liu
FoxO transcription factors are important regulators of cell survival in response to a variety of stress stimuli and play vital functions in tumor progression. However, the functions and underlying regulators of FoxO3a in colorectal cancer (CRC) have not been fully elucidated. The aim of the current study was to identify the functions of FoxO3a in CRC and characterize the transcription elements within the promoter region of FoxO3a. The expression of FoxO3a was upregulated in response to hypoxic and oxidative stress stimuli...
May 2018: Oncology Reports
Song Xu, Fan Yang, Renwang Liu, Xiongfei Li, Haiyang Fan, Jinghao Liu, Sen Wei, Gang Chen, Jun Chen, Yurong Da
Bone remodeling can be interrupted by tumor cells which leads to an inappropriate balance of osteoblasts and osteoclasts. As the progenitors of osteoblasts, mesenchymal stem cells (MSCs) have been reported to exhibit an abnormal osteogenic differentiation potential in some cancer‑related bone lesions. However, the evidence is very limited in terms of the biological alterations of MSCs in the bone metastasis of non‑small cell lung cancers (NSCLC). We investigated the expression and function of miR‑139‑5p in MSC osteogenic differentiation in vitro in normal and NSCLC-exposed condition...
May 2018: Oncology Reports
J Woo, J E Kim, J J Im, J Lee, H S Jeong, S Park, S-Y Jung, H An, S Yoon, S M Lim, S Lee, J Ma, E Y Shin, Y-E Han, B Kim, E H Lee, L Feng, H Chun, B-E Yoon, I Kang, S R Dager, I K Lyoo, C J Lee
The role of astrocytes in brain plasticity has not been extensively studied compared with that of neurons. Here we adopted integrative translational and reverse-translational approaches to explore the role of an astrocyte-specific major water channel in the brain, aquaporin-4 (AQP4), in brain plasticity and learning. We initially identified the most prevalent genetic variant of AQP4 (single nucleotide polymorphism of rs162008 with C or T variation, which has a minor allele frequency of 0.21) from a human database (n=60 706) and examined its functionality in modulating the expression level of AQP4 in an in vitro luciferase reporter assay...
April 2018: Molecular Psychiatry
Hai Shang, Zhi Qiang Hao, Xi Bo Fu, Xiang Dong Hua, Zuo Hong Ma, Fu Lu Ai, Zhao Qiang Feng, Kun Wang, Wen Xin Li, Bo Li
The proliferative activity of hepatic carcinoma cells is directly associated with tumorigenesis, tumor development, metastasis and invasion. A variety of cytokines and peptides serve important roles in the development of hepatic carcinoma. The aim of the present study was to examine the effect of intermedin (IMD) on hepatic carcinoma cell proliferation and its mechanism of action. HepG2 hepatic carcinoma cell lines were treated with human recombinant IMD1-53 and its receptor antagonist IMD17-47. Cell proliferation was detected using a Cell Counting kit-8...
April 2018: Oncology Letters
Chunhong Zhang, Guomin Song, Weisheng Ye, Baoshan Xu
Osteosarcoma is one of the most frequent types of primary malignant bone neoplasm in children and adolescents. Despite advancements developed in therapeutic modalities, the 5-year overall survival rates for patients with metastatic osteosarcoma disease remain poor. The present study aimed to investigate the expression level of microRNA-302a (miR-302a) in osteosarcoma tissues and cell lines, and the biological roles of miR-302a in osteosarcoma cells. In addition, the molecular mechanism underlying its tumor suppressive roles was evaluated...
April 2018: Oncology Letters
Yan Guo, Min Tao, Min Jiang
Increasing evidence has demonstrated that microRNAs (miRNAs) have a crucial role in the initiation and progression of tumors. The present study aimed to investigate the expression and the role of miRNA-454-3p in human cervical cancer. Human cervical cancer cells were transfected with miRNA-454-3p mimics or negative control miRNA. MTT, Transwell and wound healing assays were performed to investigate the effects of miRNA-454-3p overexpression on cell proliferation, invasion and migration, respectively. The results indicated that miRNA-454-3p was down-regulated in human cervical cancer cell lines, while its ectopic overexpression significantly inhibited their proliferation, migration and invasion...
March 2018: Experimental and Therapeutic Medicine
Zhao-Xiong Zhang, Wan-Ni Zhang, Yuan-Yuan Sun, Yun-Hui Li, Zhen-Ming Xu, Wei-Neng Fu
Purpose: CREB, MYCY1 and NAT10 are involved in cancer cell migration. However, the relationship between these three proteins and their role in laryngeal cancer cell migration remains unknown. Methods: Transient gene transfection was performed in laryngeal cancer cells. Bioinformatics analysis was used to predict the binding of CREB to MYCT1 promoter. Binding of CREB to the promoter of MYCT1 was monitored by luciferase reporter assay and chromatin immuno-precipitation method in vitro and in vivo, respectively...
2018: OncoTargets and Therapy
Bijay Dhungel, Slawomir Andrzejewski, Aparna Jayachandran, Ritu Shrestha, Charmaine A Ramlogan-Steel, Christopher J Layton, Jason C Steel
Hepatocellular carcinoma (HCC) is a major health problem as evidenced by its increasing incidence and high morbidity and mortality rates. Most patients with HCC have underlying liver disease and dysfunction which limits the current therapeutic options. Treatments that spare the liver and destroy the HCC are needed. Targeting transcriptional differences between HCC and liver cells may provide this therapeutic window. In this study, we examine the potential of the Glypican 3 (GPC3) promoter as a targeting strategy...
March 21, 2018: Gene Therapy
Xinshan Cao, Ling Xu, Quanyuan Liu, Lijuan Yang, Na Li, Xiaoxiao Li
Our study aimed to investigate the roles and possible regulatory mechanism of miR-1277 in the development of hepatocellular carcinoma (HCC). HCC patients were identified from patients who were diagnosed with focal liver lesions using magnetic resonance imaging (MRI). The expression levels of miR-1277 in the serum of HCC patients and HepG2 cells were measured. Then miR-1277 mimics, miR-1277 inhibitor and scramble RNA were transfected into cells. The effects of miR-1277 overexpression and suppression on HepG2 cell proliferation, migration and invasion were then investigated...
March 21, 2018: Oncology Research
Dandan Wu, Jun Liu, Jianliang Chen, Haiyan He, Hang Ma, Xuedong Lv
MicroRNAs(miRNAs) have been reported to be involved in many human cancers and tumor progression. The dysregulation of miR-449a is found in many types of malignancies and associated with tumor growth, migration and invasion. However, its expression and function in non-small cell lung cancer (NSCLC) still remain unclear. In our study, miR-449a was found to be down-regulated both in NSCLC tissues and cell lines, and low miR-449a expression was obviously associated with tumor differentiation, TMN stage and poor overall survival (OS)...
March 21, 2018: Oncology Research
Yang Cao, Xu Shi, Yingmin Liu, Ren Xu, Qing Ai
MicroRNA-338-3p has been reported to be a tumor suppressor in multiple cancer types. However, the biological role of miR-338-3p and underlying mechanism in multiple myeloma (MM) remains unclear. In the present study, we investigate the biological role and potential of miR-338-3p in MM. We found that miR-338-3p was significantly decreased in MM newly diagnosed and relapsed tissues and cell lines. Overexpression of miR-338-3p in MM cells significantly inhibited proliferation and promoted apoptosis, and caspase-3 and caspase-8 activity...
March 21, 2018: Oncology Research
Richard P Tan, Alex H P Chan, Katarina Lennartsson, Maria M Miravet, Bob S L Lee, Jelena Rnjak-Kovacina, Zoe E Clayton, John P Cooke, Martin K C Ng, Sanjay Patel, Steven G Wise
BACKGROUND: Induced pluripotent stem-cell derived endothelial cells (iPSC-ECs) can be generated from any somatic cell and their iPSC sources possess unlimited self-renewal. Previous demonstration of their proangiogenic activity makes them a promising cell type for treatment of ischemic injury. As with many other stem cell approaches, the low rate of in-vivo survival has been a major limitation to the efficacy of iPSC-ECs to date. In this study, we aimed to increase the in-vivo lifetime of iPSC-ECs by culturing them on electrospun polycaprolactone (PCL)/gelatin scaffolds, before quantifying the subsequent impact on their proangiogenic function...
March 21, 2018: Stem Cell Research & Therapy
Eveline Hoebe, Coral Wille, Stacy Hagemeier, Shannon Kenney, Astrid Greijer, Jaap Middeldorp
Epstein-Barr Virus (EBV) BamHI-A rightward frame 1 (BARF1) protein is considered a viral oncogene in epithelial cells and has immune-modulating properties. During viral lytic replication BARF1 is expressed as an early gene, regulated by the immediate early EBV protein R. However, in viral latency BARF1 is exclusively expressed in epithelial tumors such as nasopharyngeal (NPC) and gastric carcinoma (GC) but not in lymphomas, indicating that activation of the BARF1 promoter is cell type specific. Undifferentiated NPC is characterized by high expression of ΔNp63 isoforms of the epithelial differentiation marker p63, a member of the p53 family of transcription factors...
March 17, 2018: Cancers
Wenhui Jiang, Tianxiang Liu, Wenzhi Nan, Diddugodage Chamila Jeewani, Yanlu Niu, Chunlian Li, Yong Wang, Xue Shi, Cong Wang, Jiahuan Wang, Yang Li, Xin Gao, Zhonghua Wang
The purple pericarp of bread wheat (Triticum aestivum L.) is a useful source of dietary anthocyanins. Previous mapping results indicated that the purple pericarp is controlled by two complementary genes located on chromosomes 7D and 2A. However, the related genes and their effects on regulating the purple pericarp are unknown. In this study, two transcription factors were characterised as anthocyanin activators in purple pericarp: TaPpm1 (purple pericarp-MYB 1) and TaPpb1 (purple pericarp-bHLH 1). Three nonfunctional variants were detected in the coding sequence of TaPpm1 from non-purple seed lines, in which the function of TaPpm1 was destroyed by insertion-caused frame shift or truncated peptides...
March 17, 2018: Journal of Experimental Botany
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