keyword
MENU ▼
Read by QxMD icon Read
search

Luciferase

keyword
https://www.readbyqxmd.com/read/28550686/microrna-186-suppresses-cell-proliferation-and-metastasis-through-targeting-sentrin-specific-protease-1-in-renal-cell-carcinoma
#1
Dan Jiao, Man Wu, Lei Ji, Feng Liu, Yingying Liu
Recent evidence suggests that dysregulation of microRNAs are associated with the development of multiple malignancies.miR-186 has been reported as a critical cancer regulator in several types of cancers. However, its functional significance and molecular mechanism underlying renal cell carcinoma (RCC) remains unknown. In this study, our results showed that miR-186 expression was dramatically downregulated in RCC tissues and cell lines compared to that in adjacent normal tissues and cell lines. Overexpression of miR-186 significantly inhibited cell growth, colony formation, cell invasion, caused cell cycle arrest at G0/G1 phase, and induced cell apoptosis as detected by MTT, colony formation, Transwell assay, and flow cytometry assays in RCC cells...
May 21, 2017: Oncology Research
https://www.readbyqxmd.com/read/28550684/long-noncoding-rna-ccat2-knockdown-suppresses-tumorous-progression-by-sponging-mir-424-in-epithelial-ovarian-vancer
#2
Fu Hua, Chang-Hua Li, Xiao-Gang Chen, Xiao-Ping Liu
Epithelial ovarian cancer (EOC) is the one of most common gynecological malignant tumor with highest mortality, meanwhile, series of lncRNAs have been validated to play vital role in EOC tumorigenesis. Colon cancer-associated transcript 2 (CCAT2) has been verified as oncogenic lncRNA in multiple tumors, however, the role of CCAT2 in EOC genesis is still unclear. The purpose of present study is to probe the function of CCAT2 on EOC. Preliminary experiments found that CCAT2 expression was significantly up-regulated in EOC tissue and cell lines compared to noncancerous tissue and cells...
May 21, 2017: Oncology Research
https://www.readbyqxmd.com/read/28550682/long-noncoding-rna-anril-promotes-cervical-cancer-development-by-acting-as-a-sponge-of-mir-186
#3
Jun-Jun Zhang, Dan-Dan Wang, Chen-Xiang Du, Yan Wang
Cervical cancer is a common malignancy of female reproductive system. Long non-coding RNA (lncRNA) has been reported tomodulate tumor progression in multiple cancers. The lncRNA antisense non-coding RNA in the INK4 locus (ANRIL) has been testified as oncogenic molecular in series tumors, however, the function and underlying mechanism involved in cervical cancer oncogenesis is still unclear. In present study, RT-PCR showed that ANRIL expression was significantly up-regulated in cervical cancer tumors and cell lines...
May 22, 2017: Oncology Research
https://www.readbyqxmd.com/read/28550679/downregulation-of-src-kinase-signaling-inhibitor-1-srcin1-expression-by-microrna-32-promotes-proliferation-and-epithelial-mesenchymal-transition-in-human-liver-cancer-cells
#4
Ren Chen, Jin-Yao Liao, Jing Huang, Wen-Li Chen, Xiao-Jun Ma, Xiao-Dan Luo
MicroRNAs play an important role in regulating gene expression by binding to the 3' UTR of target mRNAs. In this studywe have made an attempt to assess the molecular mechanisms by which miR-32 suppresses the expression of SRCIN1, thereby leading to promotion of proliferation and epithelial-mesenchymal transition of human liver cancer cells. Human liver cancer cell lines HepG2 were transfected with miR-32 mimics and its control. The HepG2 cell lines were the assessed for miR-32 expression. The transfected cell lines were then studied for SRCIN1 expression by luciferase assay, effect of transfection on cell proliferation and finally epithelial-mesenchymal transition...
May 22, 2017: Oncology Research
https://www.readbyqxmd.com/read/28550492/microrna-889-is-downregulated-by-histone-deacetylase-inhibitors-and-confers-resistance-to-natural-killer-cytotoxicity-in-hepatocellular-carcinoma-cells
#5
Haitao Xie, Qiugui Zhang, Hui Zhou, Jun Zhou, Ji Zhang, Yan Jiang, Jinghong Wang, Xianglin Meng, Leping Zeng, Xiaoxin Jiang
Major histocompatibility complex class I chain-related gene B (MICB) is expressed on tumor cells and participates in natural killer (NK) cell-mediated antitumor immune response through engagement with the NKG2D receptor. This study was undertaken to identify novel microRNA (miRNA) regulators of MICB and clarify their functions in NK cell-mediated cytotoxicity to hepatocellular carcinoma (HCC) cells. Bioinformatic analysis and luciferase reporter assay were conducted to search for MICB-targeting miRNAs. Overexpression and knockdown experiments were performed to determine the roles of candidate miRNAs in the susceptibility of HCC cells to NK lysis...
May 26, 2017: Cytotechnology
https://www.readbyqxmd.com/read/28550372/tnf%C3%AE-induced-dlk-activation-contributes-to-apoptosis-in-the-beta-cell-line-hit
#6
Svenja Börchers, Rohollah Babaei, Catarina Klimpel, Jorge Duque Escobar, Sabine Schröder, Roland Blume, Muhammad Nasir Hayat Malik, Elke Oetjen
Reduction in beta-cell mass and function contributes to the pathogenesis of diabetes mellitus type 2. The proinflammatory cytokines tumor necrosis factor (TNF)α and interleukin (IL)-1β have been implicated in the pathogenesis of this disease. Overexpression of the dual leucine zipper kinase (DLK) inhibits beta-cell function and induces apoptosis in the beta-cell line HIT. In the present study, it was investigated whether TNFα or IL-1β stimulates DLK enzymatic activity. Immunoblot analysis, transient transfection with luciferase reporter gene assays, and immunofluorescence were used...
May 27, 2017: Naunyn-Schmiedeberg's Archives of Pharmacology
https://www.readbyqxmd.com/read/28549468/microrna-608-inhibits-proliferation-of-bladder-cancer-via-akt-foxo3a-signaling-pathway
#7
Zhen Liang, Xiao Wang, Xin Xu, Bo Xie, Alin Ji, Shuai Meng, Shiqi Li, Yi Zhu, Jian Wu, Zhenghui Hu, Yiwei Lin, Xiangyi Zheng, Liping Xie, Ben Liu
BACKGROUND: Current evidence indicates that miR-608 is widely down-regulated in various malignant tumors including liver cancer, colon cancer, lung cancer and glioma, and acts as a tumor suppressor by inhibiting cell proliferation, invasion and migration or by promoting apoptosis. The specific biological function of miR-608 in bladder cancer is still unknown. METHODS: qRT-PCR and Chromogenic in Situ Hybridization (CISH) was conducted to assess the expression of miR-608 in paired BCa tissues and adjacent non-tumor bladder urothelial tissues...
May 26, 2017: Molecular Cancer
https://www.readbyqxmd.com/read/28549320/light-emission-miracle-in-the-sea-and-preeminent-applications-of-bioluminescence-in-recent-new-biotechnology
#8
Sana Sharifian, Ahmad Homaei, Roohullah Hemmati, Khosro Khajeh
Bioluminescence is referred to the light emission by a living organism due to a specific biochemical reaction. This interesting feature of the organisms could highly influences behavioral and ecosystem dynamics. Luminescence, mostly observed in marine species, is generally higher in deep-living genera than in benthic or shallow organisms. However, among creatures living in land, fireflies, beetles, springtails and fungi have shown some bioluminescent activities. Classically, the emission of light is catalyzed by luciferase from a substrate...
May 17, 2017: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/28548843/zwitterionic-nanocarrier-surface-chemistry-improves-sirna-tumor-delivery-and-silencing-activity-relative-to-polyethylene-glycol
#9
Meredith A Jackson, Thomas A Werfel, Elizabeth J Curvino, Fang Yu, Taylor Elizabeth Kavanaugh, Samantha M Sarett, Mary D Dockery, Kameron V Kilchrist, Ayisha N Jackson, Todd D Giorgio, Craig L Duvall
Although siRNA-based nanomedicines hold promise for cancer treatment, conventional siRNA-polymer complex (polyplex) nanocarrier systems have poor pharmacokinetics following intravenous delivery, hindering tumor accumulation. Here, we determined the impact of surface chemistry on the in vivo pharmacokinetics and tumor delivery of siRNA polyplexes. A library of diblock polymers was synthesized, all containing the same pH-responsive, endosomolytic polyplex core-forming block but different corona blocks: 5 kDa (benchmark) and 20 kDa linear PEG, 10 kDa and 20 kDa brush-like poly(oligo ethylene glycol) (POEGMA), and 10 kDa and 20 kDa zwitterionic phosphorylcholine-based polymers (PMPC)...
May 26, 2017: ACS Nano
https://www.readbyqxmd.com/read/28548636/long-non-coding-rna-uca1-targets-mir-122-to-promote-proliferation-migration-and-invasion-of-glioma-cells
#10
Yang Sun, Jun-Gong Jin, Wei-Yang Mi, Hao Wu, Shi-Rong Zhang, Qiang Meng, Shi-Tao Zhang
Glioma is the most common and lethal intracranial malignant tumor. Long non-coding RNAs (lncRNAs) have been identifiedas pivotal regulators in the tumorigenesis of glioma. However, the role of lncRNA urothelial carcinoma-associated 1 (UCA1) in the gliomagenesis is still unknown. The purpose of this study is to investigate the underlying function of UCA1 on glioma genesis. Results demonstrated that UCA1 was up-regulated in glioma tissue and indicated the poor prognosis. UCA1 knockdown induced by si-UCA1 significantly suppressed the proliferative, migrative and invasive activity of glioma cell lines (U87 and U251)...
May 5, 2017: Oncology Research
https://www.readbyqxmd.com/read/28547952/mir-150-up-regulates-glut1-and-increases-glycolysis-in-osteosarcoma-cells
#11
Guangke Yuan, Yanqing Zhao, Dongjin Wu, Chunzheng Gao
Objective: Osteosarcoma (OS) is the most common malignant bone tumor in children and young adults. Many studies have shown that microRNAs play a critical role in proliferation and metastasis with this tumour type. However, whether aberrant expression might contribute to a metabolism switch in osteosarcoma cases is not clearly understood. In this study, we explored expression and function of miR-150 in osteosarcoma cells. Materials and methods: Expression of miR-150 was assessed by real-time PCR in cell lines and human patient tissues...
April 1, 2017: Asian Pacific Journal of Cancer Prevention: APJCP
https://www.readbyqxmd.com/read/28546856/epigenetic-regulation-of-runx2-transcription-and-osteoblast-differentiation-by-nicotinamide-phosphoribosyltransferase
#12
Min Ling, Peixin Huang, Shamima Islam, Daniel P Heruth, Xuanan Li, Li Qin Zhang, Ding-You Li, Zhaohui Hu, Shui Qing Ye
BACKGROUND: Bone degenerative disorders like osteoporosis may be initiated by age-related shifts in anabolic and catabolic responses that control bone homeostasis. Although there are studies suggesting that metabolic changes occur with stem cell differentiation, the molecular mechanisms governing energy metabolism and epigenetic modification are not understood fully. Here we reported the key role of nicotinamide phosphoribosyltransferase (Nampt), which is the rate-limiting enzyme in the salvage pathway of NAD biosynthesis from nicotinamide, in the osteogenic differentiation of bone marrow stromal cells...
2017: Cell & Bioscience
https://www.readbyqxmd.com/read/28546827/chemical-constituents-from-a-gynostemma-laxum-and-their-antioxidant-and-neuroprotective-activities
#13
Ji Yeon Seo, Sang Kyum Kim, Phi Hung Nguyen, Ju Yong Lee, Pham Ha Thanh Tung, Sang Hyun Sung, Won Keun Oh
BACKGROUND: A few bioactivities of constituents from Gynostemma laxum, which has been collected in Vietnam, have been reported until now. There is no report about the effects of constituents from G. laxum although the nuclear factor (erythroid-derived 2)-like 2 (Nrf2)-mediated heme oxygenase-1 (HO-1) antioxidant defense system is involved in neuroprotection in the brain. Therefore, we investigated whether quercetin (2), benzoic acid (10) and their analogues (1, 3-9 and 11) from G. laxum have the antioxidant and neuroprotective activities and also their underlying mechanism...
2017: Chinese Medicine
https://www.readbyqxmd.com/read/28546757/microrna-381-enhances-radiosensitivity-in-esophageal-squamous-cell-carcinoma-by-targeting-x-linked-inhibitor-of-apoptosis-protein
#14
Suna Zhou, Yaoyou Cui, Dequan Yu, Jun Liang, Mingxin Zhang, Wenguang Ye
BACKGROUND: Increasing evidence indicates that radioresistance remains a major problem in the treatment of patients with esophageal squamous cell carcinoma (ESCC). This study was designed to investigate the expression of microRNA-381 (miR-381) and its function in the radioresistance of ESCC. METHODS: In this study, miR-381 expression was first detected in ESCC cell lines and tissue samples by quantitative real-time polymerase chain reaction (qRT-PCR). Then, the effects of miR-381 expression on growth, apoptosis, and radiosensitivity of ESCC cells were analyzed by MTT, colony formation, and flow cytometry, respectively...
2017: OncoTargets and Therapy
https://www.readbyqxmd.com/read/28546229/mir-182-5p-improves-the-viability-mitosis-migration-and-invasion-ability-of-human-gastric-cancer-cells-by-down-regulating-rab27a
#15
Yuling Li, Shudong Chen, Zhengfei Shan, Liyan Bi, Shengqiang Yu, Yongwei Li, Sen Xu
We investigated the effect of miR-182-5p on the viability, proliferation, invasion and migration ability of human gastric cells by regulating the expression of RAB27A. Real-time PCR assay was used to detect the expression of miR-182-5 and RAB27A in human gastric carcinoma tissues, para-carcinoma tissues and different cell lines. Western blotting was also used to determine the RAB27A expression in both tissues and cell lines. We chose the HGC-27 cell line as experiment subject as it demonstrated the highest miR-182-5p level...
May 25, 2017: Bioscience Reports
https://www.readbyqxmd.com/read/28546132/identification-of-direct-target-genes-of-mir-7-mir-9-mir-96-and-mir-182-in-the-human-breast-cancer-cell-lines-mcf-7-and-mda-mb-231
#16
Hamidreza Moazzeni, Ali Najafi, Marzieh Khani
Some microRNAs have carcinogenic or tumor suppressive effects in breast cancer, which is the most common cancer in women worldwide. MiR-7 and miR-9 are tumor suppressor microRNAs, which induce apoptosis and inhibit proliferation in breast cancer cells. Moreover, miR-96 and miR-182 are onco-microRNAs that increase proliferation, migration, and tumorigenesis in breast cancer cells. This study aimed to identify the direct target genes of these four microRNAs in the human breast cancer cell lines MCF-7 and MDA-MB-231...
May 22, 2017: Molecular and Cellular Probes
https://www.readbyqxmd.com/read/28546024/mirna-8159-is-involved-in-tlr-signaling-pathway-regulation-after-pathogen-infection-by-direct-targeting-tlr13-in-miiuy-croaker
#17
Junxia Cui, Yunhang Gao, Qing Chu, Dekun Bi, Tianjun Xu
Toll-like receptors (TLRs) play a crucial role in the recognition of immune reactions against invading pathogens. The molecular regulation mechanisms of TLR expression in aquatic organisms remain unclear. MicroRNAs (miRNAs) are small non-coding RNAs that are critical adjustors of immune signaling pathway at the post-transcriptional level and play critical roles in intricate networks of host-pathogen interactions and innate immunity. The critical role of TLRs in host defense for discerning certain kinds of pathogen associated molecular patternsand striking a cascade immune response in fish have been demonstrated...
May 22, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28546020/the-evolution-and-functional-characterization-of-miiuy-croaker-interferon-regulatory-factor-9-involved-in-immune-response
#18
Qiong Yang, Junxia Cui, Weihua Song, Xueyan Zhao, Tianjun Xu
Interferon regulatory factors (IRFs) are transcription factors playing important roles in regulating the expression of type I interferons (IFNs) and IFN-stimulated genes. IRF9 is one of the IRF family gene members which belongs to the IRF4 subfamily. Mammalian IRF9 has been known to be involved in antiviral response as the DNA sequence recognition subunit of IFN-stimulated gene factor 3 (ISGF3) complex. In fish, only a few studies investigated the characteristics of IRF9 and the role in IFN signaling. In this study, we identify the IRF9 gene from miiuy croaker (mmiIRF9) and studied its feature and function...
May 22, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28545668/phenethyl-isothiocyanate-suppresses-cancer-stem-cell-properties-in-vitro-and-in-a-xenograft-model
#19
Ji Ho Yun, Kyung-A Kim, Gyhye Yoo, Sun Young Kim, Ji Min Shin, Jung Hoon Kim, Sang Hoon Jung, Jungho Kim, Chu Won Nho
BACKGROUND: Cancer stem cells (CSCs) are a subset of cells within the bulk of a tumor that have the ability to self-renew and differentiate, and are thus associated with cancer invasion, metastasis, and recurrence. Phenethyl isothiocyanate (PEITC) is a natural compound found in cruciferous vegetables such as broccoli and is used as a cancer chemopreventive agent; however, its effects on CSCs are little known. PURPOSE: To evaluate the effect of PEITC on CSCs in this study by examining CSC properties...
July 1, 2017: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
https://www.readbyqxmd.com/read/28545666/mahuannin-b-an-adenylate-cyclase-inhibitor-attenuates-hyperhidrosis-via-suppressing-%C3%AE-2-adrenoceptor-camp-signaling-pathway
#20
Zengyong Wang, Yu Cui, Guoyu Ding, Mengge Zhou, Xiaoyao Ma, Yuanyuan Hou, Min Jiang, Dailin Liu, Gang Bai
BACKGROUND: Based on the traditional application of traditional Chinese Medicines (TCMs), Ephedra Herba (EH) is used to cure cold fever by inducing sweating, whereas Ephedra Radix (ER) is used to treat hyperhidrosis. Although they come from the same plant, Ephedra sinica Stapf, but have play opposing roles in clinical applications. EH is known to contain ephedrine alkaloids, which is the driver of the physiological changes in sweating, heart rate and blood pressure. However, the active pharmacological ingredients (APIs) of ER and the mechanisms by which it restricts sweating remain unknown...
July 1, 2017: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
keyword
keyword
28830
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"