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https://www.readbyqxmd.com/read/29141248/association-of-functional-genetic-variants-of-hotair-with-hepatocellular-carcinoma-hcc-susceptibility-in-a-chinese-population
#1
Hao Li, Xian-Mei Tang, Yangchen Liu, Weizhen Li, Qiaoyun Chen, Yan Pan
BACKGROUND/AIMS: The HOX transcript antisense intergenic RNA (HOTAIR), a long non-coding RNA (lncRNA), plays an important role in the pathogenesis and progression of multiple tumors. The aim of the present study was to evaluate whether common single nucleotide polymorphisms (SNPs) in HOTAIR are related to hepatocellular carcinoma (HCC) susceptibility in a Chinese population. METHODS: We genotyped three SNPs of HOTAIR in a hepatocellular carcinoma (HCC) case-control study, including 482 cases and 520 control subjects...
November 15, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29141136/systemic-mrna-delivery-to-the-lungs-by-functional-polyester-based-carriers
#2
Yunfeng Yan, Hu Xiong, Xinyi Zhang, Qiang Cheng, Daniel John Siegwart
Messenger RNA (mRNA) has recently come into focus as an emerging therapeutic class with great potential for protein replacement therapy, cancer immunotherapy, regenerative medicine, vaccines, and gene editing. However, the lack of effective and safe delivery methods impedes the broad application of mRNA-based therapeutics. Here, we report a robust approach to develop efficient polymeric delivery carriers for mRNA. Lead polyesters were identified by in vitro screening of a 480-member combinatorially modified poly(trimethylolpropane allyl ether-co-suberoyl chloride) library for the delivery of luciferase encoding mRNA (Luc mRNA) to IGROV1 cells...
November 15, 2017: Biomacromolecules
https://www.readbyqxmd.com/read/29140695/rna-g-quadruplexes-in-kirsten-ras-kras-oncogene-as-targets-for-small-molecules-inhibiting-translation
#3
Giulia Miglietta, Susanna Cogoi, Jessica Marinello, Giovanni Capranico, Alexander S Tikhomirov, Andrey E Shchekotikhin, Luigi E Xodo
The human KRAS transcript contains a G-rich 5'-UTR sequence (77 % GC) harbouring several G4 motifs capable to form stable RNA G-quadruplex (RG4) structures that can serve as targets for small molecules. A biotin-streptavidin pull-down assay showed that 4,11-bis(2-aminoethylamino)anthra[2,3-b]furan-5,10-dione (2a) binds to RG4s in the KRAS transcript under low-abundance cellular conditions. Dual-luciferase assays demonstrated that 2a and its analogue 4,11-bis(2-aminoethylamino)anthra[2,3-b]thiophene-5,10-dione 2b) repress translation in a dose-dependent manner...
November 15, 2017: Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/29139022/effect-of-plasma-activated-lactated-ringer-s-solution-on-pancreatic-cancer-cells-in-vitro-and-in-vivo
#4
Yusuke Sato, Suguru Yamada, Shigeomi Takeda, Norifumi Hattori, Kae Nakamura, Hiromasa Tanaka, Masaaki Mizuno, Masaru Hori, Yasuhiro Kodera
BACKGROUND: The medical applications of nonequilibrium atmospheric pressure plasma in cancer therapy have attracted attention. We previously reported on the antitumor effect of plasma-activated medium. However, this approach requires plasma-activated liquids that are administrable to the human body. In this study, we produced plasma-activated lactated Ringer's solution (PAL) and evaluated its antitumor effect and mechanism. Furthermore, we evaluated the effect of the intraperitoneal administration of PAL using a peritoneal dissemination mouse tumor model...
November 14, 2017: Annals of Surgical Oncology
https://www.readbyqxmd.com/read/29138985/effects-of-microrna-708-on-epithelial-mesenchymal-transition-cell-proliferation-and-apoptosis-in-melanoma-cells-by-targeting-lef1-through-the-wnt-signaling-pathway
#5
Xiao-Fei Song, Qi-Hua Wang, Ran Huo
This study was conducted in order to elucidate the role microRNA-708 (miR-708) plays between proliferation, invasion, migration, and epithelial-mesenchymal transition (EMT) involving melanoma cells by targeting using LEF1 through the Wnt signaling pathway. Male Kunming mice were selected and subsequently divided into normal and model groups to take part in this study. Following cell line selection, the B16 cells with the highest miR-708 expression were selected and assigned into the control, blank, negative control (NC), miR-708 mimic, miR-708 inhibitor, siRNA-LEF1, and miR-708 inhibitor + siRNA-LEF1 groups...
November 14, 2017: Pathology Oncology Research: POR
https://www.readbyqxmd.com/read/29138833/mir-16-mimics-inhibit-tgf-%C3%AE-1-induced-epithelial-to-mesenchymal-transition-via-activation-of-autophagy-in-non-small-cell-lung-carcinoma-cells
#6
Hui Wang, Ying Zhang, Qian Wu, Yong-Bin Wang, Wei Wang
Autophagy is critical for the metastasis of cancer cells through induction of epithelial-to-mesenchymal transition (EMT). Activation of TGF-β signaling plays a key role in regulating autophagy. miR-16 may be associated with non-small cell lung carcinoma (NSCLC) progression. However, the role of miR-16 in NSCLC cell autophagy in the presence of TGF-β and the underlying mechanism are still unclear. To test whether miR-16 targets ATG3 which is involved in autophagy of NSCLC cells, we studied the expression levels of miR-16 and ATG3 in NSCLC patients, verified the targeting of ATG3 by miR-16 by luciferase reporter gene system, and investigated the role of miR-16 in the autophagy of NSCLC cells...
November 9, 2017: Oncology Reports
https://www.readbyqxmd.com/read/29138830/mir%C3%A2-185-inhibits-non%C3%A2-small-cell-lung-cancer-cell-proliferation-and-invasion-through-targeting-of-sox9-and-regulation-of-wnt-signaling
#7
Zhengwen Lei, Hongcan Shi, Wei Li, Duonan Yu, Feiyang Shen, Xi Yu, Dan Lu, Chao Sun, Kai Liao
SRY-box 9 (SOX9) is an important transcription factor required for development, which has additionally been reported to be an independent prognostic indicator for the survival of patients with non‑small cell lung cancer (NSCLC). Accumulating evidence has indicated that dysregulation of microRNAs (miRNAs/miRs) may contribute to the initiation and progression of cancer. SOX9 may be regulated by a number of miRNAs in different types of cancer, including in NSCLC. The present study sought to identify novel candidate miRNAs associated with SOX9 in NSCLC using online tools, and investigated the detailed functions of miR‑185, which suppressed SOX9 mRNA expression most strongly out of the candidate miRNAs...
November 14, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29138807/role-of-lasp-1-a-novel-sox9-transcriptional-target-in-the-progression-of-lung-cancer
#8
Jianguang Shi, Jing Guo, Xinjian Li
Lung cancer accounts for most cancer-related deaths worldwide. However, the underlying mechanism by which it mediates the progression of lung cancer remains unclear. Expression of LASP-1 (LIM and SH3 protein 1) was evaluated in lung cancer tissues and tumor-adjacent normal tissues using immunohistochemistry and western blotting. Functional studies have shown that siRNA-mediated silencing of LASP-1 in human lung cancer cells and reduced cell proliferation, migration, and invasion. Flow cytometry and immunofluorescence staining also revealed that rate of cell apoptosis was increased after knockdown of expression of LASP-1, thereby suggesting that LASP-1 may function as an oncogene during lung cancer progression...
November 10, 2017: International Journal of Oncology
https://www.readbyqxmd.com/read/29138751/expression-of-microrna-29a-regulated-by-yes-associated-protein-modulates-the-neurite-outgrowth-in-n2a-cells
#9
Chunye Tan, Changlin Yu, Zhiwen Song, Hongjun Zou, Xu Xu, Jinbo Liu
Yes-associated protein (YAP) is proved to increase miR-29a in the present study, but the relevant molecular mechanism is not clear. Also, growing evidence indicates that the high-level miR-29a promotes the neurite outgrowth by decreasing PTEN (phosphatase and tensin homologue deleted on chromosome 10). Results show that the expression of miR-29a increases but the PTEN decreases during transfecting the N2a cells with the YAP plasmid. Meanwhile, the advancement of neurite outgrowth is presented via using multiple methods to detect the expression of GAP-43 and NF-200, which have a strong association with neurite outgrowth...
2017: BioMed Research International
https://www.readbyqxmd.com/read/29138357/tadalafil-a-long-acting-phosphodiesterase-inhibitor-promotes-bone-marrow-stem-cell-survival-and-their-homing-into-ischemic-myocardium-for-cardiac-repair
#10
Ibrahim Elmadbouh, Muhammad Ashraf
The aim was to evaluate the tadalafil-mediated effects at molecular level on bone marrow-derived mesenchymal stem cells (MSCs) survival and their homing into the infarcted hearts to promote cardiac repair and improve function. MSCs were pretreated in vitro with inhibitors of PKG, MAPK, FasL, nitric oxide synthase (NOS) (L-NAME), CXCR4 (AMD3100), or miR-21 inhibitors (+/-luciferase construction +/-Fas) prior to tadalafil treatment for 2 h. These MSCs were then subjected to H2O2 stress to assess their injury...
November 2017: Physiological Reports
https://www.readbyqxmd.com/read/29137983/transcriptional-regulation-of-acetoacetyl-coa-synthetase-by-sp1-in-neuroblastoma-cells
#11
Shinya Hasegawa, Masahiko Imai, Masahiro Yamasaki, Noriko Takahashi, Tetsuya Fukui
Acetoacetyl-CoA synthetase (AACS) is the enzyme responsible for cholesterol and fatty acid synthesis in the cytosol. We have previously shown that AACS has an important role in normal neuronal development and that knockdown of SREBP-2, which orchestrates cholesterol synthesis, resulted in the downregulation of AACS mRNA levels. In this study, we investigated the transcriptional mechanism of AACS in Neuro-2a, neuroblastoma cells. Luciferase assay showed that the minimal core promoter of the mouse AACS gene is located in a region with 110 bps upstream from the transcription start site...
November 11, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29137866/development-of-noviomimetics-that-modulate-molecular-chaperones-and-manifest-neuroprotective-effects
#12
Leah K Forsberg, Mercy Anyika, Zhenyuan You, Sean Emery, Mason McMullen, Rick T Dobrowsky, Brian S J Blagg
Heat shock protein 90 (Hsp90) is a chaperone under investigation for the treatment of cancer and neurodegenerative diseases. Neuroprotective Hsp90 C-terminal inhibitors derived from novobiocin (novologues) include KU-32 and KU-596. These novologues modulate molecular chaperones and result in an induction of Heat Shock Protein 70 (Hsp70). "Noviomimetics" replace the synthetically complex noviose sugar with a simple cyclohexyl moiety to maintain biological efficacy as compared to novologues KU-596 and KU-32...
October 18, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/29137689/microrna-433-represses-proliferation-and-invasion-of-colon-cancer-cells-by-targeting-homeobox-a1
#13
Heming Li, Junfeng Li, Taisheng Yang, Shuwen Lin, Heng Li
The aberrant expression of miR-433 has been validated in some types of cancers. However, the expression profile and thebiological function of miR-433 on colon cancer are still elusive. This study was designed to investigate the function of miR-433 on proliferation and invasion of colon cancer cell. We detected the expression of miR-433 in colon cancer tissues, adjacent normal tissues and cell lines. CCK8 and transwell assays were performed to explore the impact of miR-433 on colon cancer cell proliferation and invasion...
October 3, 2017: Oncology Research
https://www.readbyqxmd.com/read/29137404/the-role-of-tfpi2-hypermethylation-in-the-detection-of-gastric-and-colorectal-cancer
#14
Haochang Hu, Xiaoying Chen, Cheng Wang, Yuting Jiang, Jingjing Li, Xiuru Ying, Yong Yang, Bin Li, Cong Zhou, Jie Zhong, Dongping Wu, Jieer Ying, Shiwei Duan
Gastrointestinal cancer is a prevalent disease with high morbidity and mortality. Tissue factor pathway inhibitor 2 (TFPI2) gene could protect the extracellular matrix of cancer cells from degradation and tumor invasion. The goal of our study was to estimate the diagnostic value of TFPI2 hypermethylation in gastric cancer (GC) and colorectal cancer (CRC). TFPI2 methylation was measured by quantitative methylation-specific polymerase chain reaction (qMSP) method in 114 GC and 80 CRC tissues and their paired non-tumor tissues...
October 13, 2017: Oncotarget
https://www.readbyqxmd.com/read/29137403/chicken-ccdc152-shares-an-nfyb-regulated-bidirectional-promoter-with-a-growth-hormone-receptor-antisense-transcript-and-inhibits-cells-proliferation-and-migration
#15
Shudai Lin, Wei Luo, Mingya Jiang, Wen Luo, Bahareldin Ali Abdalla, Qinghua Nie, Li Zhang, Xiquan Zhang
The chicken coiled-coil domain-containing protein 152 (CCDC152) recently has been identified as a novel one implicated in cell cycle regulation, cellular proliferation and migration by us. Here we demonstrate that CCDC152 is oriented in a head-to-head configuration with the antisense transcript of growth hormone receptor (GHR) gene. Through serial luciferase reporter assays, we firstly identified a minimal 102 bp intergenic region as a core bidirectional promoter to drive basal transcription in divergent orientations...
October 13, 2017: Oncotarget
https://www.readbyqxmd.com/read/29137386/mitochondrial-calcium-uniporter-as-a-target-of-microrna-340-and-promoter-of-metastasis-via-enhancing-the-warburg-effect
#16
Changhui Yu, Yuhao Wang, Jiawen Peng, Qiang Shen, Mimi Chen, Wei Tang, Xiumei Li, Chunqing Cai, Bin Wang, Shaoxi Cai, Xiaojing Meng, Fei Zou
Background: A shift from oxygen phosphorylation to aerobic glycolysis was known as the Warburg effect and a characteristic of cancer cell metabolism facilitating metastasis. Mitochondrial calcium uniporter (MCU), a key ion channel that mediates Ca(2+) uptake into mitochondria, was found to promote cancer progression and metastasis. However, its explicit role in shifting metabolism of breast cancer cells has not been defined. Methods: We evaluated MCU overexpression or knock-down on migration, invasion and glucose metabolismin breast cancer cells...
October 13, 2017: Oncotarget
https://www.readbyqxmd.com/read/29137305/mir-93-3p-inhibition-suppresses-clear-cell-renal-cell-carcinoma-proliferation-metastasis-and-invasion
#17
Lu Wang, Guang Yang, Xiangwei Zhu, Ziqi Wang, Hongzhi Wang, Yang Bai, Pengcheng Sun, Li Peng, Wei Wei, Guang Chen, Guangbin Li, Andrey A Zamyatnin, Peter V Glybochko, Wanhai Xu
miRNA dysregulation is associated with many human diseases, including cancer. This study explored the effects of miR-93-3p on clear cell renal cell carcinoma (ccRCC). We found that miR-93-3p is upregulated an average of 38-fold in 138 ccRCC specimens compared to matched normal kidney tissues, which correlated with poor patient outcome. miR-93-3p inhibition reduced ccRCC cell growth, invasion, and migration in vitro and in a mouse xenograft model. A search of the TargetScan, miRanda, and PicTar databases revealed that miR-93-3p is predicted to regulate pigment epithelium-derived factor (PEDF)...
October 10, 2017: Oncotarget
https://www.readbyqxmd.com/read/29137282/functional-polymorphisms-in-the-promoter-region-of-mir-17-92-cluster-are-associated-with-a-decreased-risk-of-colorectal-cancer
#18
Ruifen Sun, Yundan Liang, Fang Yuan, Xinwen Nie, Hong Sun, Yanyun Wang, Tao Yu, Linbo Gao, Lin Zhang
miR-17-92 cluster is identified as a potential oncogenic miRNA. The aim of this study was to investigate the association of polymorphisms in the promoter region of miR-17-92 cluster with the risk of colorectal cancer (CRC). Three polymorphisms (i.e., rs9588884, rs982873 and rs1813389) in the promoter of miR-17-92 were analyzed among 874 cases and 1132 controls using a TaqMan allelic discrimination assay or a polymerase chain reaction-restriction fragment length polymorphism method. Relative expression of miR-17-92 was examined among CRC tumors and noncancerous tissues using quantitative reverse transcription-PCR...
October 10, 2017: Oncotarget
https://www.readbyqxmd.com/read/29137244/microrna-423-5p-facilitates-hypoxia-reoxygenation-induced-apoptosis-in-renal-proximal-tubular-epithelial-cells-by-targeting-gstm1-via-endoplasmic-reticulum-stress
#19
Xiao-Peng Yuan, Long-Shan Liu, Chuan-Bao Chen, Jian Zhou, Yi-Tao Zheng, Xiao-Ping Wang, Ming Han, Chang-Xi Wang
It has been reported that microRNAs (miRs) can regulate renal response to acute injury and members of them are believed to be important in maintenance of renal function and development of renal injury. We investigated the actions of microRNA-423-5p (miR-423-5p) and glutathione-S-transferase (GST) M1 after acute kidney injury. MiR-423-5p was up-regulated and GSTM1 was down-regulated in human kidney (HK-2) cells subjected to hypoxia/reoxygenation (H/R) and in rat kidneys subjected to ischemia/reperfusion (I/R) injury...
October 10, 2017: Oncotarget
https://www.readbyqxmd.com/read/29136958/mir-182-5p-regulates-bcl2l12-and-bcl2-expression-in-acute-myeloid-leukemia-as-a-potential-therapeutic-target
#20
Suwei Zhang, Qiaoxin Zhang, Ganggang Shi, Jun Yin
The importance of microRNAs (miRNAs) are shown during various cancers including acute myeloid leukemia (AML). MiR-182-5p functions as an oncogene or a potential suppressive miRNA in cancers, but its expression and function in AML is unknown. The purpose is to investigate the roles of miR-182-5p in AML in this study. MiR-182-5p was examined in the blood samples of AML and it was found that miR-182-5p expression levels were higher in AML tissues than it in their normal controls, so did in the AML cells. BCL2L12 and BCL2 were predicted as target genes of miR-182-5p and verified using luciferase reporter assay...
November 10, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
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