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https://www.readbyqxmd.com/read/28945006/microrna-let-7-targets-the-ecdysone-signaling-pathway-e75-gene-to-control-larval-pupal-development-in-bactrocera-dorsalis
#1
Wei Peng, Wei-Wei Zheng, Kaleem Tariq, Shu-Ning Yu, Hong-Yu Zhang
MicroRNAs (miRNAs) regulate various biological processes during insect development; however, their role in larval-pupal development in oriental fruit fly, Bactrocera dorsalis (Hendel) remains unknown. In current study, we address the biological function of a conserved miRNA, Bdo-Let-7 in the regulation of BdE75 gene, which belongs to ecdysone signaling pathway and participates in the larval-pupal development in B. dorsalis. Using dual luciferase reporter assay in HEK293T cells we show that Bdo-Let-7 miRNA interacts with the 3' UTR of BdE75 gene and suppresses its expression...
September 25, 2017: Insect Science
https://www.readbyqxmd.com/read/28944911/microrna%C3%A2-3666-inhibits-breast-cancer-cell-proliferation-by-targeting-sirtuin-7
#2
Di Li, Lifei Li
The abnormal expression of microRNAs (miRNAs) is associated with cancer initiation and progression. miRNAs functioning as oncogenes or tumor suppressors represent novel biomarkers for cancer diagnosis, prognosis, and serve as therapeutic tools. MiR‑3666 has been reported as a tumor suppressor in various types of cancer; however, its role in breast cancer remains unknown. In the current study, the aim was to investigate the potential role of miR‑3666 in breast cancer. It was identified that miR‑3666 was decreased in breast cancer cell lines and that the overexpression of miR‑3666 inhibited breast cancer cell proliferation...
September 25, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28944896/microrna%C3%A2-186-targets-igf%C3%A2-1r-and-exerts-tumor%C3%A2-suppressing-functions-in-glioma
#3
Jian Jiang, Weijie Wang, Dazhao Fang, Xiaodong Jin, Lianshu Ding, Xiaoyang Sun
Glioma is the most common malignant brain tumor in adults and represents one of the most aggressive and life‑threatening types of cancer in humans. Increasing studies have revealed that microRNAs are abnormally expressed in various types of human cancer, and have oncogenic or tumor suppressive roles, which depend primarily on the type of cancer. The present study aimed to investigate the expression level and effects of microRNA‑186 (miR‑186) on glioma, and its underlying molecular mechanism. Reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) analysis was performed to detect the expression of miR‑186 in glioma tissues and cell lines...
September 21, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28944868/microrna%C3%A2-198-contributes-to-lupus-nephritis-progression-by-inhibition-of-phosphatase-and-tensin-homology-deleted-on-chromosome-ten-expression
#4
Danyu Cui, Dingji Zhu, Hao Ren, Jingli Lin, Weinan Lai, Qin Huang, Jinjun Zhao, Min Yang
A number of short noncoding microRNAs (miRs) have been demonstrated to be highly expressed in many kidney diseases such as renal cancer and lupus nephritis (LN); however, these results have not been extensively investigated. The aim of the present study was to investigate the expression and function of miR‑198 in LN based on the previous studies. miR‑198 expression level in systemic lupus erythematosus (SLE) patients was determined to determine its clinicopathological significance and effect on glomerular cell proliferation...
September 19, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28944858/decreased-expression-of-hsa%C3%A2-mir%C3%A2-372-predicts-poor-prognosis-in-patients-with-gallbladder-cancer-by-affecting-chloride-intracellular-channel-1
#5
Ning Zhou, Wei Cheng, Chuang Peng, Yi Liu, Bo Jiang
It has been reported that hsa‑microRNA (miRNA/miR)‑372 functions as a tumor suppressor or oncogene in various digestive system tumors, however, its roles in gallbladder cancer (GBC) are yet to be established. The present study aimed to determine the expression and clinical relevance of hsa‑miR‑372 in GBC. The expression of hsa‑miR‑372 in 80 pairs of human GBC tissues and adjacent normal gallbladder tissues was measured by reverse transcription‑quantitative polymerase chain reaction. Subsequently, the associations between hsa‑miR‑372 expression levels and the clinicopathological characteristics of patients with GBC were determined using χ2 test...
September 19, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28944851/microrna%C3%A2-96-regulates-the-proliferation-of-nucleus-pulposus-cells-by-targeting-arid2-akt-signaling
#6
Bo Tao, Jiaoyu Yi, Changzhao Huang, Wang Xu, Chao Qin, Lei Chen, Jincai Chen, Yan Gao, Ruiying Wang
The aberrant proliferation of nucleus pulposus (NP) cells has been reported to be implicated in the pathogenesis of intervertebral disc degeneration (IDD). Previous studies have demonstrated that microRNAs (miRNAs), which are a group of small noncoding RNAs, are critical regulators of cell proliferation in various pathologies. However, the role of miRNA‑96 (miR‑96) in the proliferation of NP cells remains to be determined. In the present study, reverse transcription‑quantitative polymerase chain reaction was used to investigate the expression of miR‑96 in NP tissues from patients with IDD and healthy tissues from patients with traumatic lumbar fracture as the control...
September 20, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28944848/cmip-is-oncogenic-in-human-gastric-cancer-cells
#7
Jianlin Zhang, Jin Huang, Xingyu Wang, Weidong Chen, Qinqing Tang, Maoyong Fang, Yeben Qian
Gastric cancer is one of the most common cancers and the second leading cause of cancer‑associated mortality worldwide. Recurrence, metastasis and resistance to drug treatment are the main barrier to survival of patients with advanced stage gastric cancer. Further study of the molecular mechanisms involved will improve the therapeutic options for gastric cancer. In a previous study, c‑Maf was discovered as an oncogene transduced in the avian AS42 retrovirus, and was found to be overexpressed in multiple myeloma and angioimmunoblastic T‑cell lymphoma...
September 20, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28944841/microrna%C3%A2-138%C3%A2-5p-regulates-neural-stem-cell-proliferation-and-differentiation-in%C3%A2-vitro-by-targeting-trip6-expression
#8
Juan Wang, Jixia Li, Jian Yang, Lianguo Zhang, Shane Gao, Fei Jiao, Maoli Yi, Jun Xu
Research on neural stem cells (NSCs) has recently focused on microRNAs (miRNAs), a class of small non‑coding RNAs that have crucial roles in regulating NSC proliferation and differentiation. In the present study, a quantitative‑polymerase chain reaction assay revealed that the expression of miRNA (miR)‑138‑5p was significantly decreased during neural differentiation of NSCs in vitro. Overexpression of miR‑138‑5p reduced NSC proliferation and increased NSC differentiation. Furthermore, suppression of miR‑138‑5p via transfection with a miRNA inhibitor enhanced NSC proliferation and attenuated NSC differentiation...
September 18, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28943945/microrna-126-inhibits-cell-viability-and-invasion-in-a-diabetic-retinopathy-model-via-targeting-irs-1
#9
Shifeng Fang, Xiang Ma, Suping Guo, Jianmin Lu
Diabetic retinopathy (DR) is a sight-threatening complication of diabetes. IRS-1 was predicted to be the target gene of microRNA-126 (miR-126). The present study was designed to illustrate the involvement of miR-126 in the regulation of DR via targeting IRS-1. The present study revealed that the expression of miR-126 was significantly decreased while IRS-1 expression was increased in endothelial cells (ECs) and retinal pericytes (RPs) from a DR mouse model compared with healthy controls. Furthermore, a luciferase reporter assay confirmed the interaction between miR-126 and IRS-1...
October 2017: Oncology Letters
https://www.readbyqxmd.com/read/28943942/honokiol-inhibits-bladder-cancer-cell-invasion-through-repressing-src-3-expression-and-epithelial-mesenchymal-transition
#10
Lan Shen, Fang Zhang, Ruimin Huang, Jun Yan, Bing Shen
Urinary bladder cancer (UBC) is one of the most common urological cancer types. Muscle invasive bladder cancer possesses high propensity for metastasis with poor prognosis. Honokiol is a lignan isolated from Magnolia officinalis with high bioavailability and potent anticancer effects. The results of the present study demonstrated that honokiol significantly inhibited UBC cell migration and invasion in a dose-dependent manner compared with the vehicle-treated control group. In addition, honokiol treatment suppressed epithelial-mesenchymal transition by induction of E-cadherin and repression of N-cadherin...
October 2017: Oncology Letters
https://www.readbyqxmd.com/read/28943901/mir-590-promotes-the-proliferation-of-humscs-and-induces-ecm-synthesis-by-targeting-smad7
#11
Tianming Liu, Yinge Wu, Tengyi Huang, Xuexuan Zhang, Yingmu Cai
MicroRNA (miR)-590 has been established to be a promoter of cell proliferation, migration and invasion, and an inhibitor of apoptosis in numerous cancer cell lines. However, its effects on non-cancer cells remain to be elucidated. miR-590 was transfected into human umbilical cord mesenchymal stem cells (HUMSCs), and the cell proliferation rate was determined using a Cell-Light 5-ethynyl-20-deoxyuridine Apollo 567 kit and the presence of extracellular matrix (ECM) proteins were detected using western blot analysis and immunofluorescence microscopy...
October 2017: Oncology Letters
https://www.readbyqxmd.com/read/28943435/lncrna-gm15290-sponges-mir-27b-to-promote-ppar%C3%AE-induced-fat-deposition-and-contribute-to-body-weight-gain-in-mice
#12
Weihua Liu, Chunli Ma, Bin Yang, Chunyan Yin, Beining Zhang, Yanfeng Xiao
We found that Gm15290 was one of the most upregulated lncRNAs in the adipose of ob/ob mice through lncRNA microarray analysis. Then, manipulations of overexpression and silencing in mouse primary adipocytes showed that Gm15290 positively regulated adipogenesis, manifested by increasing lipid deposition and upregulating adipogenic genes including PPARγ, C/EBPα, and aP2. However, overexpression of mutant Gm15290 (at the binding site of miR-27c) did not have an promoting effect on adipogenesis. Additionally, Gm15290 was found to potentially interact with miR-27b that had been identified as a PPARγ targeting miRNA, and we verified their interaction by luciferase activity and RNA pull down assays...
September 21, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28943409/interleukin-1-%C3%AE-induced-smad2-3-linker-modifications-are-tak1-dependent-and-delay-tgf%C3%AE-signaling-in-primary-human-mesenchymal-stem-cells
#13
Guus G van den Akker, Henk M van Beuningen, Elly L Vitters, Marije I Koenders, Fons A de Loo, Peter L van Lent, Esmeralda N Blaney Davidson, Peter M van der Kraan
BACKGROUND: Chondrogenic differentiation of mesenchymal stem cells (MSC) requires transforming growth factor beta (TGFβ) signaling. TGFβ binds to the type I receptor activin-like kinase (ALK)5 and results in C-terminal SMAD2/3 phosphorylation (pSMAD2/3C). In turn pSMAD2/3C translocates to the nucleus and regulates target gene expression. Inflammatory mediators are known to exert an inhibitory effect on MSC differentiation. In this study we investigated the effect of interleukin 1 β (IL1β) on SMAD2/3 signaling dynamics and post-translational modifications...
September 21, 2017: Cellular Signalling
https://www.readbyqxmd.com/read/28943214/down-regulation-of-mir-605-promotes-the-proliferation-and-invasion-of-prostate-cancer-cells-by-up-regulating-en2
#14
Yao-Jun Zhou, Hong-Qiong Yang, Wei Xia, Li Cui, Ren-Fang Xu, Hao Lu, Zhong Xue, Bo Zhang, Zi-Nong Tian, Yun-Jie Cao, Zhao-Yu Xing, Shuai Yin, Xiao-Zhou He
AIMS: MicroRNA served as inhibitor for gene expression in various cancers. This study aimed to investigate the role of miR-605 and EN2 in prostate cancer (PCa). MATERIALS AND METHODS: In this research, the expression of miR-605 and EN2 protein in PCa tissues and cells were determined by qRT-PCR and western blot, respectively. The cell proliferation was measured by Cell Counting Kit-8 (CCK-8) and the tumor cell invasion assay was accomplished with transwell system...
September 21, 2017: Life Sciences
https://www.readbyqxmd.com/read/28942451/the-human-rna-surveillance-factor-upf1-modulates-gastric-cancer-progression-by-targeting-long-non-coding-rna-malat1
#15
Li Li, Yingying Geng, Ru Feng, Qinqin Zhu, Bei Miao, Jiang Cao, Sujuan Fei
BACKGROUND/AIMS: The long non-coding RNA metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) is overexpressed in numerous cancers. However, whether MALAT1 is regulated and the related mechanisms in gastric cancer remain unclear. METHODS: Immunohistochemistry and qRT-PCR analyses were used to detect the expression levels of UPF1 and MALAT1 in gastric cancer and adjacent normal tissues. MTT, cell cycle, apoptosis and transwell assays were performed to examine the effects of UPF1 on cell cycle progression, cell proliferation, apoptosis, migration and invasion...
August 15, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28942448/antagomirs-targeting-microrna-134-increase-limk1-levels-after-experimental-seizures-in-vitro-and-in-vivo
#16
Jiahang Sun, Xiaoying Gao, Dawei Meng, Yang Xu, Xichun Wang, Xin Gu, Mian Guo, Xiaodong Shao, Hongwen Yan, Chuanlu Jiang, Yongri Zheng
BACKGROUND: MiR-134 is enriched in dendrites of hippocampal neurons and plays crucial roles in the progress of epilepsy. The present study aims to investigate the effects of antagomirs targeting miroRNA-134 (Ant-134) on limk1 expression and the binding of miR-134 and limk1 in experimental seizure. METHODS: Status epilepticus (SE) rat model was established by lithium chloride-pilocarpine injection and was treated with Ant-134 by intracerebroventricular injection...
September 22, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28942223/baicalin-suppresses-il-1%C3%AE-induced-expression-of-inflammatory-cytokines-via-blocking-nf-%C3%AE%C2%BAb-in-human-osteoarthritis-chondrocytes-and-shows-protective-effect-in-mice-osteoarthritis-models
#17
Chunhui Chen, Chuanxu Zhang, Leyi Cai, Huanguang Xie, Wei Hu, Te Wang, Di Lu, Hua Chen
Osteoarthritis (OA) is a degenerative joint disease with an inflammatory component that drives the degradation of cartilage extracellular matrix. Baicalin, a predominant flavonoid isolated from the dry root of Scutellaria baicalensis Georgi, has been reported to have anti-inflammatory effects. However, the anti-inflammatory effects of baicalin on OA have not been reported. Our study aimed to investigate the effect of baicalin on OA both in vitro and in vivo. In vitro, human OA chondrocytes were pretreated with baicalin (10, 50, 100μM) for 2h and subsequently stimulated with IL-1β for 24h...
September 21, 2017: International Immunopharmacology
https://www.readbyqxmd.com/read/28941804/mir-132-promotes-the-proliferation-invasion-and-migration-of-human-pancreatic-carcinoma-by-inhibition-of-the-tumor-suppressor-gene-pten
#18
REVIEW
Hui Zhang, Aixiang Liu, Xielin Feng, Lang Tian, Wentao Bo, Haiqing Wang, Yong Hu
MicroRNA (miRNAs) emerges as key oncogene or tumor suppressor in a variety of cancers including pancreatic carcinoma. In this study, we detected the role of miR-132 in development and progression of pancreatic cancer and the underlying mechanism. First, the expression of miR-132 in pancreatic carcinoma and adjacent non-cancerous tissues were detected by qRT-PCR. Then, the role of miR-132 in biological function of pancreatic carcinoma cells was investigated. Our results identified that miR-132 was generally upregulated in pancreatic carcinoma, and phosphatase and tensin homolog (PTEN) was generally downregulated...
September 20, 2017: Progress in Biophysics and Molecular Biology
https://www.readbyqxmd.com/read/28941704/mir-194-is-involved-in-morphogenesis-of-spiral-ganglion-neurons-in-inner-ear-by-rearranging-actin-cytoskeleton-via-targeting-rhob
#19
Jintao Du, Xuemei Zhang, Hui Cao, Di Jiang, Xianren Wang, Wei Zhou, Kaitian Chen, Jiao Zhou, Hongyan Jiang, Luo Ba
Many microRNAs participate in the development, differentiation and function preservation of the embryonic and adult inner ear, but many details still need to be elucidated regarding the numerous microRNAs in the inner ear. Based on previous investigations on the microRNA profile in the inner ear, we confirmed that several microRNAs are expressed in the inner ear, and we detected the spatial expression of these microRNAs in the neonatal mouse inner ear. Then we focused on miR-194 for its specific expression with a dynamic spatiotemporal pattern during inner ear development...
September 20, 2017: International Journal of Developmental Neuroscience
https://www.readbyqxmd.com/read/28941036/association-of-donor-sumo4-rs237025-genetic-variant-with-tacrolimus-elimination-in-the-early-period-after-liver-transplantation
#20
Tao Zhang, Yuan Liu, Rong Zeng, Qi Ling, Peihao Wen, Junwei Fan, Zhihai Peng
BACKGROUNDS & AIMS: Individualized tacrolimus treatment can improve drug safety and efficacy. In this study, we aimed to investigate the association of donor and recipient small ubiquitin-like modifier 4 (SUMO4) rs237025 polymorphisms with tacrolimus elimination and the potential mechanism. METHODS: A total of 297 liver transplant patients were enrolled in the study. CYP3A5 rs776746 and SUMO4 rs237025 were genotyped using TaqMan SNPs assays. The activity of nuclear factor-kB (NF-kB) was evaluated by luciferase assay...
September 21, 2017: Liver International: Official Journal of the International Association for the Study of the Liver
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