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Enterobacteriaceae

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https://www.readbyqxmd.com/read/28644357/effect-of-synbiotic-on-the-gut-microbiota-of-cesarean-delivered-infants-a-randomized-double-blind-multicenter-study
#1
Mei Chin Chua, Kaouther Ben-Amor, Christophe Lay, Anne G E Neo, Wei Chin Chiang, Rajeshwar Rao, Charmaine Chew, Surasith Chaithongwongwatthana, Nipon Khemapech, Jan Knol, Voranush Chongsrisawat
We determined the effect of short-chain galacto-oligosaccharides (scGOS), long-chain fructo-oligosaccharides (lcFOS) and Bifidobacterium breve M-16V on the gut microbiota of cesarean-born infants. Infants were randomized to receive a standard formula (control), the same with scGOS/lcFOS and B. breve M-16V (synbiotic), or with scGOS/lcFOS (prebiotic) from birth until week 16, 30 subjects born vaginally were included as a reference group. Synbiotic supplementation resulted in a higher bifidobacteria proportion from day 3/5 (P < 0...
July 2017: Journal of Pediatric Gastroenterology and Nutrition
https://www.readbyqxmd.com/read/28643188/impact-of-appropriateness-of-empiric-therapy-on-outcomes-in-community-onset-bacteremia-by-extended-spectrum-%C3%AE-lactamase-producing-escherichia-coli-and-klebisella-pneumoniae-definitively-treated-with-carbapenems
#2
E-J Joo, D A Park, N R Lee, S-Y Moon, J-K Choi, J-H Ko, K R Peck
Despite a significant increase of bloodstream infection caused by extended-spectrum-β-lactamase (ESBL)-producing Enterobacteriaceae in the community-setting, information regarding clinical outcomes of inappropriate empiric therapy (IAT) in patients with those infections is limited. A multicenter-retrospective cohort study was conducted in four hospitals. A total of 249 adults were identified to have community-onset bacteremia caused by ESBL-producing Escherichia coli and Klebsiella pneumoniae, and definitively treated with carbapenems...
June 23, 2017: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/28642971/high-diversity-of-bacterial-communities-in-developmental-stages-of-bactrocera-carambolae-insecta-tephritidae-revealed-by-illumina-miseq-sequencing-of-16s-rrna-gene
#3
Hoi-Sen Yong, Sze-Looi Song, Kah-Ooi Chua, Phaik-Eem Lim
Bactrocera carambolae is a highly polyphagous fruit pest of agricultural importance. This study reports the bacterial communities associated with the developmental stages of B. carambolae. The microbiota of the developmental stages were investigated by targeted 16S rRNA gene (V3-V4 region) sequencing using the Illumina MiSeq. At 97% similarity, there were 19 bacterial phyla and unassigned bacteria, comprising 39 classes, 86 orders, 159 families and 311 genera. The bacterial composition varied among the specimens of developmental stage and across developmental stages as well as exuviae...
June 23, 2017: Current Microbiology
https://www.readbyqxmd.com/read/28642846/decreased-fitness-and-virulence-in-st10-escherichia-coli-harboring-blandm-5-and-mcr-1-against-a-st4981-strain-with-blandm-5
#4
Yawei Zhang, Kang Liao, Hua Gao, Qi Wang, Xiaojuan Wang, Henan Li, Ruobing Wang, Hui Wang
Although coexistence of blaNDM-5 and mcr-1 in Escherichia coli has been reported, little is known about the fitness and virulence of such strains. Three carbapenem-resistant Escherichia coli (GZ1, GZ2, and GZ3) successively isolated from one patient in 2015 were investigated for microbiological fitness and virulence. GZ1 and GZ2 were also resistant to colistin. To verify the association between plasmids and fitness, growth kinetics of the transconjugants were performed. We also analyzed genomic sequences of GZ2 and GZ3 using PacBio sequencing...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28642140/emergence-of-carbapenem-resistant-enterobacteriaceae-isolated-from-patients-in-a-university-hospital-in-saudi-arabia-epidemiology-clinical-profiles-and-outcomes
#5
Fawzia E Alotaibi, Elham E Bukhari, Maha M Al-Mohizea, Taghreed Hafiz, Eman Bin Essa, Yasmeen I AlTokhais
Carbapenemase-producing Enterobacteriaceae have been steadily spreading worldwide during the last decade. Nine patients were identified prospectively and were followed during their hospitalization course to identify the epidemiology, clinical profiles and outcomes. These patients had one or more cultures positive for a CRE isolate, contributing to a total of eleven positive cultures from various sites without including duplicates of isolates obtained from the same site. Isolates from these patients included five Klebseilla pneumoniae, three Escherichia coli, and one Enterobacter aerogenes...
June 19, 2017: Journal of Infection and Public Health
https://www.readbyqxmd.com/read/28641596/types-and-prevalence-of-extended-spectrum-beta-lactamase-producing-enterobacteriaceae-in-poultry
#6
Eva-Maria Saliu, Wilfried Vahjen, Jürgen Zentek
For several billion years, bacteria have developed mechanisms to resist antibacterial substances. In modern time, antibiotics are frequently used in veterinary and human medicine for prevention and treatment of diseases, globally still also for their growth promoting effects as feed additives. This complex situation has evolved in accelerating development and prevalence of multi-drug resistant bacteria in livestock and people. Extended-spectrum beta-lactamase (ESBL) producing bacteria are resistant to a wide range of ß-lactam antibiotics...
June 23, 2017: Animal Health Research Reviews
https://www.readbyqxmd.com/read/28639543/rapid-detection-of-extended-spectrum-%C3%AE-lactamase-producing-enterobacteriaceae-in-blood-cultures-using-the-esbl-ndp-test-in-cotonou-benin
#7
Dissou Affolabi, Fredéric Sogbo, Gracieux Laleye, Jeanne Orekan, Faridath Massou, Aderemi Kehinde, Séverin Anagonou
PURPOSE: Rapid and inexpensive tests for detecting extended-spectrum-β-lactamase (ESBL)-producing Enterobacteriaceae are needed, particularly in low-resource countries where infections with these bacteria constitute a major public health issue. The recently described ESBL NDP test performed well in developed countries. This study was designed to assess performance, cost and feasibility of this test in positive blood cultures, in Cotonou, Benin (West Africa). METHODOLOGY: The test was performed on 175 positive Bactec broth blood cultures containing Enterobacteriaceae, and blindly compared with the double-disc synergy test (DDST) for the phenotypic detection of ESBL producers...
June 21, 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28639165/molecular-characterization-of-intestinal-carriage-of-carbapenem-resistant-enterobacteriaceae-among-inpatients-at-two-iranian-university-hospitals-first-report-of-co-production-of-bla-ndm-7-and-bla-oxa-48
#8
H Solgi, F Badmasti, Z Aminzadeh, C G Giske, M Pourahmad, F Vaziri, S A Havaei, F Shahcheraghi
Gastrointestinal colonization of carbapenem-resistant Enterobacteriaceae (CRE) could serve as a reservoir for the transmission of these pathogens in the clinical setting. The aim of this study was to investigate the intestinal carriage of CRE and to analyze risk factors for CRE carriage. Rectal swabs were collected from 95 patients at two Iranian university hospitals. CRE screening was performed using selective media (CHROMagar and MacConkey agar). Polymerase chain reaction (PCR) was used to detect carbapenemase-encoding genes...
June 21, 2017: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/28639162/bacterial-genome-sequencing-in-clinical-microbiology-a-pathogen-oriented-review
#9
REVIEW
F Tagini, G Greub
In recent years, whole-genome sequencing (WGS) has been perceived as a technology with the potential to revolutionise clinical microbiology. Herein, we reviewed the literature on the use of WGS for the most commonly encountered pathogens in clinical microbiology laboratories: Escherichia coli and other Enterobacteriaceae, Staphylococcus aureus and coagulase-negative staphylococci, streptococci and enterococci, mycobacteria and Chlamydia trachomatis. For each pathogen group, we focused on five different aspects: the genome characteristics, the most common genomic approaches and the clinical uses of WGS for (i) typing and outbreak analysis, (ii) virulence investigation and (iii) in silico antimicrobial susceptibility testing...
June 21, 2017: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/28638093/fatty-acid-composition-and-phospholipid-types-used-in-infant-formulas-modifies-the-establishment-of-human-gut-bacteria-in-germ-free-mice
#10
Rikke Guldhammer Nejrup, Tine Rask Licht, Lars Ingvar Hellgren
Human milk fat contains high concentrations of medium-chained fatty acids (MCFA) and triacylglycerols emulsified by a sphingomyelin-rich phospholipid membrane (milk phospholipids, MPL). Infant formula comprises mainly long-chained fatty acids (LCFA) emulsified with dairy proteins and soy lecithin (SL) lacking sphingomyelin. Sphingomyelin content and saturation level of phospholipids affect the gut lipase activity, which alters the concentrations of lipid hydrolysis products in ileum and colon, and hereby putatively affects the competitive advantage of specific gut bacteria...
June 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28637265/importance-of-carbapenemase-production-detection-in-carbapenem-resistant-enterobacteriaceae-looking-beyond-epidemiological-purposes
#11
Adriana Jimenez, Lilian Abbo, Merhawi T Gebrezgi
No abstract text is available yet for this article.
June 16, 2017: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/28636975/prevalence-of-extended-spectrum-%C3%AE-lactamase-producing-enterobacteriaceae-and-methicillin-resistant-staphylococcus-aureus-in-pig-farms-in-switzerland
#12
Julia G Kraemer, João Pires, Marianne Kueffer, Eulalia Semaani, Andrea Endimiani, Markus Hilty, Anne Oppliger
The presence of Methicillin-Resistant Staphylococcus aureus (MRSA) in pig farms has been widely reported, and the emergence of extended-spectrum β-lactamase-producing Enterobacteriaceae (ESBL-E) has been documented in several countries. However, data for Switzerland are very scarce. This study aimed to compare changes in the prevalence of MRSA in Swiss pig farms between 2008 and 2015 and make the first ever estimates of the presence of ESBL-E and carbapenemase producers in pigs and pig farm workers. Results showed that ESBL-E was present in both pigs and farm workers and that the proportion of farms with MRSA had increased fourfold in seven years (from 7% to 31%)...
June 18, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28636668/microbiota-in-anorexia-nervosa-the-triangle-between-bacterial-species-metabolites-and-psychological-tests
#13
Francesca Borgo, Alessandra Riva, Alberto Benetti, Maria Cristina Casiraghi, Sara Bertelli, Stefania Garbossa, Simona Anselmetti, Silvio Scarone, Antonio E Pontiroli, Giulia Morace, Elisa Borghi
Anorexia nervosa (AN) is a psychiatric disease with devastating physical consequences, with a pathophysiological mechanism still to be elucidated. Metagenomic studies on anorexia nervosa have revealed profound gut microbiome perturbations as a possible environmental factor involved in the disease. In this study we performed a comprehensive analysis integrating data on gut microbiota with clinical, anthropometric and psychological traits to gain new insight in the pathophysiology of AN. Fifteen AN women were compared with fifteen age-, sex- and ethnicity-matched healthy controls...
2017: PloS One
https://www.readbyqxmd.com/read/28636666/molecular-and-microbiological-report-of-a-hospital-outbreak-of-ndm-1-carrying-enterobacteriaceae-in-mexico
#14
Paola Bocanegra-Ibarias, Elvira Garza-González, Rayo Morfín-Otero, Humberto Barrios, Licet Villarreal-Treviño, Eduardo Rodríguez-Noriega, Ulises Garza-Ramos, Santiago Petersen-Morfin, Jesus Silva-Sanchez
OBJECTIVES: To characterize the microbiological, molecular and epidemiological data of an outbreak of carbapenem-resistant Enterobacteriaceae (CRE) in a tertiary-care hospital in Mexico. METHODS: From September 2014 to July 2015, all CRE clinical isolates recovered during an outbreak in the Hospital Civil "Fray Antonio Alcalde" in Jalisco, Mexico were screened for antimicrobial susceptibility, carbapenemase production, carbapenemase-encoding genes, and plasmid profiles...
2017: PloS One
https://www.readbyqxmd.com/read/28636609/genomic-and-phenotypic-characterisation-of-fluoroquinolone-resistance-mechanisms-in-enterobacteriaceae-in-durban-south-africa
#15
John Osei Sekyere, Daniel Gyamfi Amoako
Resistance to fluoroquinolones (FQ) is being increasingly reported and found to be mediated by efflux pumps, plasmid-mediated quinolone resistance genes (PMQR) and mutations in gyrA, gyrB, parC and parE. However, studies reporting on FQ resistance mechanisms (FQRM), particularly in Africa, are focused mostly on Salmonella. This study used a whole-genome-based approach to describe FQRM in forty-eight clinical Enterobacteriaceae isolates comprising of Klebsiella pneumoniae (n = 21), Serratia marcescens (n = 12), Enterobacter spp...
2017: PloS One
https://www.readbyqxmd.com/read/28634558/plesiomonas-shigelloides-an-unusual-cause-of-septic-abortion
#16
Gilbert Cornut, Xavier Marchand-Senecal, Christiane Gaudreau, Jeremie Berdugo, Gilles Gariepy, Catherine Tremblay, Patrice Savard
Plesiomonas shigelloides, the only oxidase-positive Enterobacteriaceae, is an inhabitant of freshwater and estuary ecosystems. We report the first possible case of Plesiomonas shigelloides-induced septic abortion. This 24-year-old female was successfully treated by dilatation and curettage as well as antimicrobial therapy.
2017: Case Reports in Infectious Diseases
https://www.readbyqxmd.com/read/28631634/phenotypic-and-molecular-characterization-of-cefotaximases-temoniera-and-sulfhydryl-variable-%C3%AE-lactamases-in-pseudomonas-and-acinetobacter-isolates-in-an-indian-tertiary-health-care-center
#17
Sana Jamali, Mohammed Shahid, Farrukh Sobia, Anuradha Singh, Haris M Khan
BACKGROUND: Cefotaximases (CTX-M), temoniera (TEM), and sulfhydryl variable (SHV) constitute a rapidly growing cluster of enzymes that have disseminated geographically. They are spreading to species other than Enterobacteriaceae and might be responsible for the presence of blaCTX-M,blaTEM, and blaSHVgenes in Pseudomonas and Acinetobacter spp. The present study was designed to characterize CTX-M, TEM, and SHV phenotypically and genotypically in Pseudomonas and Acinetobacter spp. MATERIALS AND METHODS: A total of 90 isolates (73 Pseudomonas and 17 Acinetobacter spp...
April 2017: Indian Journal of Pathology & Microbiology
https://www.readbyqxmd.com/read/28631587/dietary-supplementation-with-a-nucleotide-rich-yeast-extract-modulates-gut-immune-response-and-microflora-in-weaned-pigs-in-response-to-a-sanitary-challenge
#18
S M Waititu, F Yin, R Patterson, A Yitbarek, J C Rodriguez-Lecompte, C M Nyachoti
An experiment was carried out to evaluate the short-term effect of supplementing a nucleotide-rich yeast extract (NRYE) on growth performance, gut structure, immunity and microflora of piglets raised under sanitary and unsanitary conditions. A total of 84, 21-day old piglets were used in this study; 42 piglets were raised in a room designated as the clean room that was washed once per week, whereas the other 42 piglets were raised in a room designated as the unclean room in which 7 kg of manure from the sow herd was spread on each pen floor on day 1 and 7 and the room was not washed throughout the experiment...
June 20, 2017: Animal: An International Journal of Animal Bioscience
https://www.readbyqxmd.com/read/28631411/function-of-bacterial-community-dynamics-in-the-formation-of-cadaveric-semiochemicals-during-in-situ-carcass-decomposition
#19
Javier Pascual, Christian von Hoermann, Ann-Marie Rottler-Hoermann, Omer Nevo, Alicia Geppert, Johannes Sikorski, Katharina J Huber, Sandra Steiger, Manfred Ayasse, Jörg Overmann
The decomposition of dead mammalian tissue involves a complex temporal succession of epinecrotic bacteria. Microbial activity may release different cadaveric volatile organic compounds which in turn attract other key players of carcass decomposition such as scavenger insects. To elucidate the dynamics and potential functions of epinecrotic bacteria on carcasses, we monitored bacterial communities developing on still-born piglets incubated in different forest ecosystems by combining high-throughput Illumina 16S rRNA sequencing with gas chromatography-mass spectrometry of volatiles...
June 19, 2017: Environmental Microbiology
https://www.readbyqxmd.com/read/28630686/fecal-carriage-of-extended-spectrum-%C3%AE-lactamase-and-carbapenemase-producing-enterobacteriaceae-in-egyptian-patients-with-community-onset-gastrointestinal-complaints-a-hospital-based-cross-sectional-study
#20
H M Abdallah, N Alnaiemi, E A Reuland, B B Wintermans, A Koek, A M Abdelwahab, A Samy, K W Abdelsalam, C M J E Vandenbroucke-Grauls
OBJECTIVES: The aim of this study was to determine the prevalence of extended-spectrum β-lactamase (ESBL) and carbapenemase production among Enterobacteriaceae isolated from ambulatory patients with gastrointestinal complaints admitted to El-Ahrar General Hospital, Zagazig, Egypt in the period between January 2013 and May 2013. METHODS: One hundred and thirteen Enterobacteriaceae isolates were recovered from 100 consecutive Egyptian patients with community-onset gastrointestinal complaints...
2017: Antimicrobial Resistance and Infection Control
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