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https://www.readbyqxmd.com/read/27522494/hsp90-and-hsp47-appear-to-play-an-important-role-in-minnow-puntius-sophore-for-surviving-in-the-hot-spring-run-off-aquatic-ecosystem
#1
Arabinda Mahanty, Gopal Krishna Purohit, Ravi Prakash Yadav, Sasmita Mohanty, Bimal Prasanna Mohanty
Changes in the expression of a number of hsp genes in minnow Puntius sophore collected from a hot spring run-off (Atri hot spring in Odisha, India; 20(o)09'N 85(°)18'E, 36-38 °C) were investigated to study the upper thermal acclimation response under heat stress, using same species from aquaculture ponds (water temperature 27 °C) as control. Expression of hsp genes was analyzed in both groups using RT-qPCR, which showed up-regulation of hsp90 (2.1-fold) and hsp47 (2.5-fold) in hot spring run-off fishes, whereas there was no alteration in expression of other hsps...
August 13, 2016: Fish Physiology and Biochemistry
https://www.readbyqxmd.com/read/26994673/immunotoxicological-effects-of-cadmium-on-labeo-rohita-with-emphasis-on-the-expression-of-hsp-genes
#2
Sib Sankar Giri, Shib Sankar Sen, Jin Woo Jun, Venkatachalam Sukumaran, Se Chang Park
The present study evaluated the effects of exposure (28 days) to a sub-lethal concentration of cadmium (Cd) (0.65 mg CdCl2 L(-1)) on the immune responses and expression of immune-related and heat shock protein (HSP) genes in Labeo rohita, an important aquacultured fish species. Among the immune parameters studied, significantly lower lysozyme activity was observed in fish 28 days post-exposure (dpe) to Cd as compared to control fish. Alternative complement pathway activity was slightly higher in the Cd-exposed group at 2 dpe than in controls, and this activity declined gradually thereafter...
July 2016: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/25710177/metabolic-and-chaperone-gene-loss-marks-the-origin-of-animals-evidence-for-hsp104-and-hsp78-chaperones-sharing-mitochondrial-enzymes-as-clients
#3
Albert J Erives, Jan S Fassler
The evolution of animals involved acquisition of an emergent gene repertoire for gastrulation. Whether loss of genes also co-evolved with this developmental reprogramming has not yet been addressed. Here, we identify twenty-four genetic functions that are retained in fungi and choanoflagellates but undetectable in animals. These lost genes encode: (i) sixteen distinct biosynthetic functions; (ii) the two ancestral eukaryotic ClpB disaggregases, Hsp78 and Hsp104, which function in the mitochondria and cytosol, respectively; and (iii) six other assorted functions...
2015: PloS One
https://www.readbyqxmd.com/read/25652292/immunomodulatory-effect-of-arsenic-on-cytokine-and-hsp-gene-expression-in-labeo-rohita-fingerlings
#4
Sudeshna Banerjee, Tandrima Mitra, Gopal Krishna Purohit, Sasmita Mohanty, Bimal Prasanna Mohanty
Immune system is fundamental for survival of an organism against invading pathogens and other harmful agents. Cytokines, the signaling proteins that are produced transiently after cell activation and exert pleiotropic effects on cells of the immune system, are important mediators of cell mediated immune response. When expressed in a dysregulated fashion cytokines can underlie either immunodeficient or immunopathologic states. Heat shock proteins (stress proteins, HSPs) are also key proteins, which play important role in immunomodulation, apoptosis and influence the immune responses...
May 2015: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/25003111/investigating-hsp-gene-expression-in-liver-of-channa-striatus-under-heat-stress-for-understanding-the-upper-thermal-acclimation
#5
Gopal Krishna Purohit, Arabinda Mahanty, Mrutyunjay Suar, Anil Prakash Sharma, Bimal Prasanna Mohanty, Sasmita Mohanty
Changes in hsp gene expression profiles in murrel Channa striatus experimentally exposed to temperature stress (36°C) for 4, 15, and 30 days were investigated; fish collected from aquaculture ponds and maintained in laboratory at the pond temperature (25 ± 1°C) served as control. Channa collected from a hot spring runoff (36°C) was included in the study to examine the hsp profiles beyond 30 days of exposure. Gene expression analyses of a battery of hsps in liver tissues were carried out by quantitative RT-PCR and protein expressions were analyzed by immunoblotting...
2014: BioMed Research International
https://www.readbyqxmd.com/read/24659933/resveratrol-attenuates-exercise-induced-adaptive-responses-in-rats-selectively-bred-for-low-running-performance
#6
Nikolett Hart, Linda Sarga, Zsolt Csende, Lauren G Koch, Steven L Britton, Kelvin J A Davies, Zsolt Radak
Low capacity runner (LCR) rats have been developed by divergent artificial selection for treadmill endurance capacity to explore an aerobic biology-disease connection. The beneficial effects of resveratrol supplementation have been demonstrated in endurance running. In this study it was examined whether 12 weeks of treadmill exercise training and/or resveratrol can retrieve the low running performance of the LCR and impact mitochondrial biogenesis and quality control. Resveratrol regressed running performance in trained LCR (p<0...
January 2014: Dose-response: a Publication of International Hormesis Society
https://www.readbyqxmd.com/read/23422033/resveratrol-enhances-exercise-training-responses-in-rats-selectively-bred-for-high-running-performance
#7
Nikolett Hart, Linda Sarga, Zsolt Csende, Erika Koltai, Lauren G Koch, Steven L Britton, Kelvin J A Davies, Dimitris Kouretas, Barbara Wessner, Zsolt Radak
High Capacity Runner (HCR) rats have been developed by divergent artificial selection for treadmill endurance running capacity to explore an aerobic biology-disease connection. The beneficial effects of resveratrol supplementation have been demonstrated in endurance running and the antioxidant capacity of resveratrol is also demonstrated. In this study we examine whether 12 weeks of treadmill exercise training and/or resveratrol can enhance performance in HCR. Indeed, resveratrol increased aerobic performance and strength of upper limbs of these rats...
November 2013: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/23042129/pleiotropic-roles-of-the-msi1-like-protein-msl1-in-cryptococcus-neoformans
#8
Dong-Hoon Yang, Shinae Maeng, Anna K Strain, Anna Floyd, Kirsten Nielsen, Joseph Heitman, Yong-Sun Bahn
Msi1-like (MSIL) proteins contain WD40 motifs and have a pleiotropic cellular function as negative regulators of the Ras/cyclic AMP (cAMP) pathway and components of chromatin assembly factor 1 (CAF-1), yet they have not been studied in fungal pathogens. Here we identified and characterized an MSIL protein, Msl1, in Cryptococcus neoformans, which causes life-threatening meningoencephalitis in humans. Notably, Msl1 plays pleiotropic roles in C. neoformans in both cAMP-dependent and -independent manners largely independent of Ras...
December 2012: Eukaryotic Cell
https://www.readbyqxmd.com/read/22720784/the-saccharomyces-cerevisiae-hot1p-regulated-gene-yhr087w-hgi1-has-a-role-in-translation-upon-high-glucose-concentration-stress
#9
M Gomar-Alba, E Jiménez-Martí, M del Olmo
BACKGROUND: While growing in natural environments yeasts can be affected by osmotic stress provoked by high glucose concentrations. The response to this adverse condition requires the HOG pathway and involves transcriptional and posttranscriptional mechanisms initiated by the phosphorylation of this protein, its translocation to the nucleus and activation of transcription factors. One of the genes induced to respond to this injury is YHR087W. It encodes for a protein structurally similar to the N-terminal region of human SBDS whose expression is also induced under other forms of stress and whose deletion determines growth defects at high glucose concentrations...
2012: BMC Molecular Biology
https://www.readbyqxmd.com/read/22319614/role-of-the-hahog1-map-kinase-in-response-of-the-conifer-root-and-butt-rot-pathogen-heterobasidion-annosum-to-osmotic-and-oxidative-stress-corrected
#10
Tommaso Raffaello, Susanna Keriö, Fred O Asiegbu
The basidiomycete Heterobasidion annosum (Fr.) Bref. s.l. is a filamentous white rot fungus, considered to be the most economically important pathogen of conifer trees. Despite the severity of the tree infection, very little is known about the molecular and biochemical aspects related to adaptation to abiotic stresses. In this study, the osmotic and oxidative tolerance as well as the role of the HaHOG1 Mitogen Activated Protein Kinase (MAPK) gene were investigated. The transcript levels of the yeast orthologues GPD1, HSP78, STL1, GRE2 and the ATPase pumps ENA1, PMR1, PMC1 known to have an important role in osmotolerance were also quantified under salt osmotic conditions...
2012: PloS One
https://www.readbyqxmd.com/read/19723579/mitochondrial-protein-homeostasis-the-cooperative-roles-of-chaperones-and-proteases
#11
REVIEW
Wolfgang Voos
Mitochondria contain an endogenous set of chaperones and proteases that form a complex and functionally interconnected protein quality control system responsible for maintenance of mitochondrial enzyme content and function (protein homeostasis). Here the functional roles of the ATP-dependent protease Pim1/LON and the ClpB-type chaperone Hsp78, both members of the ubiquitous AAA+ (ATPases associated with a wide variety of cellular activities) protein family, are described and discussed in the context of protein homeostasis processes under normal and stress conditions...
November 2009: Research in Microbiology
https://www.readbyqxmd.com/read/17227293/varying-responses-of-human-cells-with-discrepant-p53-activity-to-ionizing-radiation-and-heat-shock-exposure
#12
S V Tokalov, S Pieck, H O Gutzeit
OBJECTIVES: Both heat shock (HS) and ionizing radiation have an impact on the cell cycle and may induce cell cycle arrest or apoptosis. Mutations of the p53 gene are observed at a high frequency in human tumours and are recognized in about half of all human cancers. Sensitivity to radiation, heat and anticancer agents has been observed in p53(+/+) cells, but not in mutated or p53-deficient cells. Moreover, enhancement of radiosensitivity by HS has been observed in wild-type p53 cells but not in p53-deficient cells...
February 2007: Cell Proliferation
https://www.readbyqxmd.com/read/16765060/structure-and-function-of-hsp78-the-mitochondrial-clpb-homolog
#13
Claudia Leidhold, Birgit von Janowsky, Dorothea Becker, Tom Bender, Wolfgang Voos
The cellular role of Hsp100/Clp chaperones in maintaining protein stability is based on two functional aspects. Under normal growth conditions they represent components of cellular protein quality control machineries that selectively remove damaged or misfolded polypeptides in cooperation with specific proteases. After thermal stress, proteins of the ClpB subfamily have the unique ability to directly resolubilize aggregated polypeptides in concert with Hsp70-type chaperones, leading to the recovery of enzymatic activity...
October 2006: Journal of Structural Biology
https://www.readbyqxmd.com/read/16563798/the-molecular-chaperone-hsp104-a-molecular-machine-for-protein-disaggregation
#14
REVIEW
Benjamin Bösl, Valerie Grimminger, Stefan Walter
At the Cold Spring Harbor Meeting on 'Molecular Chaperones and the Heat Shock Response' in May 1996, Susan Lindquist presented evidence that a chaperone of yeast termed Hsp104, which her group had been investigating for several years, is able to dissolve protein aggregates (Glover, J.R., Lindquist, S., 1998. Hsp104, Hsp70, and Hsp40: a novel chaperone system that rescues previously aggregated proteins. Cell 94, 73-82). Among many of the participants this news stimulated reactions reaching from decided skepticism to utter disbelief because protein aggregation was widely considered to be an irreversible process...
October 2006: Journal of Structural Biology
https://www.readbyqxmd.com/read/16545993/hsp78-chaperone-functions-in-restoration-of-mitochondrial-network-following-heat-stress
#15
Agnieszka Lewandowska, Magdalena Gierszewska, Jaroslaw Marszalek, Krzysztof Liberek
Under physiological conditions mitochondria of yeast Saccharomyces cerevisiae form a branched tubular network, the continuity of which is maintained by balanced membrane fusion and fission processes. Here, we show using mitochondrial matrix targeted green fluorescent protein that exposure of cells to extreme heat shock led to dramatic changes in mitochondrial morphology, as tubular network disintegrated into several fragmented vesicles. Interestingly, this fragmentation did not affect mitochondrial ability to maintain the membrane potential...
February 2006: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/16489413/systematic-analysis-of-hsp-gene-expression-and-effects-on-cell-growth-and-survival-at-high-hydrostatic-pressure-in-saccharomyces-cerevisiae
#16
Takeshi Miura, Hiroaki Minegishi, Ron Usami, Fumiyoshi Abe
We systematically investigated the role of HSP genes in the growth and survival of Saccharomyces cerevisiae under high hydrostatic pressure together with analysis of pressure-regulated gene expression. Cells of strain BY4742 were capable of growth at moderate pressure of 25 MPa. When pressure of 25 MPa was applied to the cells, the expression of HSP78, HSP104, and HSP10 was upregulated by about 3- to 4-fold, and that of HSP32, HSP42, and HSP82 was upregulated by about 2- to 2.6-fold. However, the loss of one of the six genes did not markedly affect growth at 25 MPa, while the loss of HSP31 impaired high-pressure growth...
August 2006: Extremophiles: Life Under Extreme Conditions
https://www.readbyqxmd.com/read/16460754/the-disaggregation-activity-of-the-mitochondrial-clpb-homolog-hsp78-maintains-hsp70-function-during-heat-stress
#17
Birgit von Janowsky, Tamara Major, Karin Knapp, Wolfgang Voos
Molecular chaperones are important components of mitochondrial protein biogenesis and are required to maintain the organellar function under normal and stress conditions. We addressed the functional role of the Hsp100/ClpB homolog Hsp78 during aggregation reactions and its functional cooperation with the main mitochondrial Hsp70, Ssc1, in mitochondria of the yeast Saccharomyces cerevisiae. By establishing an aggregation/disaggregation assay in intact mitochondria we demonstrated that Hsp78 is indispensable for the resolubilization of protein aggregates generated by heat stress under in vivo conditions...
March 31, 2006: Journal of Molecular Biology
https://www.readbyqxmd.com/read/16339719/regulation-and-recovery-of-functions-of-saccharomyces-cerevisiae-chaperone-bip-kar2p-after-thermal-insult
#18
Laura Seppä, Marja Makarow
We described earlier a novel mode of regulation of Hsp104, a cytosolic chaperone directly involved in the refolding of heat-denatured proteins, and designated it delayed upregulation, or DUR. When Saccharomyces cerevisiae cells grown at the physiological temperature of 24 degrees C, preconditioned at 37 degrees C, and treated briefly at 50 degrees C were shifted back to 24 degrees C, Hsp104 expression was strongly induced after 2.5 h of recovery and returned back to normal after 5 h. Here we show that the endoplasmic reticulum (ER) chaperones BiP/Kar2p and Lhs1p and the mitochondrial chaperone Hsp78 were also upregulated at the physiological temperature during recovery from thermal insult...
December 2005: Eukaryotic Cell
https://www.readbyqxmd.com/read/14757377/weak-electromagnetic-fields-50-hz-elicit-a-stress-response-in-human-cells
#19
Sergey V Tokalov, Herwig O Gutzeit
The aim of this study was to demonstrate the expression of heat shock (HS) genes in human cells in response to extremely low-frequency electromagnetic fields (ELF-EMF) alone and in combination with thermal stress. After exposing human myeloid leukemia (HL-60) cells to the stressor(s) for 30 min we quantified the expression of the HS genes HSP27, HSP60, HSP70 (A, B, and C), HSC70, HSP75, HSP78, and HSP90 (alpha and beta) by RT-PCR. The results clearly show that HS genes, in particular the three HSP70 genes (A, B, and C), are induced by ELF-EMF, a reaction that is enhanced by simultaneous HS (43 degrees C for 30 min)...
February 2004: Environmental Research
https://www.readbyqxmd.com/read/14680327/gene-expression-profiling-in-ecotoxicology
#20
REVIEW
Terry W Snell, Sara E Brogdon, Michael B Morgan
Gene expression profiling is a powerful new end point for ecotoxicology and a means for bringing the genomics revolution to this field. We review the usefulness of gene expression profiling as an end point in ecotoxicology and describe methods for applying this approach to non-model organisms. Since genomes contain thousands of genes representing hundreds of pathways, it is possible to identify toxicant-specific responses from this wide array of possibilities. Stressor-specific signatures in gene expression profiles can be used to diagnose which stressors are impacting populations in the field...
December 2003: Ecotoxicology
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