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https://www.readbyqxmd.com/read/28425146/a-deep-nasopharyngeal-swab-versus-nonendoscopic-bronchoalveolar-lavage-for-isolation-of-bacterial-pathogens-from-preweaned-calves-with-respiratory-disease
#1
L Van Driessche, B R Valgaeren, L Gille, F Boyen, R Ducatelle, F Haesebrouck, P Deprez, B Pardon
BACKGROUND: Nonendoscopic bronchoalveolar lavage (BAL) is a practical alternative for a deep nasopharyngeal swab (DNS) to sample the airways of a large number of calves in a short period of time. The extent of commensal overgrowth and agreement of BAL with DNS culture results in preweaned calves are unknown. OBJECTIVES: To compare commensal overgrowth and bacterial culture results between DNS and BAL samples. ANIMALS: A total of 183 preweaned calves (144 with bovine respiratory disease and 39 healthy animals)...
April 19, 2017: Journal of Veterinary Internal Medicine
https://www.readbyqxmd.com/read/28416833/-diagnosis-and-treatment-for-the-basicervical-fractures-of-the-trochanteric-region
#2
T C Zhang, Z S Zhang, F Zhou, Y Tian, H Q Ji, Y Guo, Y Lv, Z W Yang, G J Hou
OBJECTIVE: To evaluate the rate of basicervical fractures and document their diagnosis and treatment. METHODS: From January 2005 to May 2016, 28 basicervical fractures of the 832 trochanteric fractures were collected and evaluated. The patients were treated with multiple screws, dynamic hip screw (DHS), intramedullary nail. Via the operation time, postoperative hospitalization, loss of blood duration the operation, hidden blood loss, total blood loss, mean union time and the final follow-up Harris hip score, the characteristics of different internal fixations were compared and analyzed...
April 18, 2017: Beijing da Xue Xue Bao. Yi Xue Ban, Journal of Peking University. Health Sciences
https://www.readbyqxmd.com/read/28415324/scale-dependence-of-multiplier-distributions-for-particle-concentration-enstrophy-and-dissipation-in-the-inertial-range-of-homogeneous-turbulence
#3
Thomas Hartlep, Jeffrey N Cuzzi, Brian Weston
Turbulent flows preferentially concentrate inertial particles depending on their stopping time or Stokes number, which can lead to significant spatial variations in the particle concentration. Cascade models are one way to describe this process in statistical terms. Here, we use a direct numerical simulation (DNS) dataset of homogeneous, isotropic turbulence to determine probability distribution functions (PDFs) for cascade multipliers, which determine the ratio by which a property is partitioned into subvolumes as an eddy is envisioned to decay into smaller eddies...
March 2017: Physical Review. E
https://www.readbyqxmd.com/read/28407487/matching-nlr-immune-receptors-to-autoimmunity-in-camta3-mutants-using-antimorphic-nlr-alleles
#4
Signe Lolle, Christiaan Greeff, Klaus Petersen, Milena Roux, Michael Krogh Jensen, Simon Bressendorff, Eleazar Rodriguez, Kenneth Sømark, John Mundy, Morten Petersen
To establish infection, pathogens deploy effectors to modify or remove host proteins. Plant immune receptors with nucleotide-binding, leucine-rich repeat domains (NLRs) detect these modifications and trigger immunity. Plant NLRs thus guard host "guardees." A corollary is that autoimmunity may result from inappropriate NLR activation because mutations in plant guardees could trigger corresponding NLR guards. To explore these hypotheses, we expressed 108 dominant-negative (DN) Arabidopsis NLRs in various lesion mimic mutants, including camta3, which exhibits autoimmunity...
April 12, 2017: Cell Host & Microbe
https://www.readbyqxmd.com/read/28392766/presence-and-absence-of-muscle-contraction-elicited-by-peripheral-nerve-electrical-stimulation-differentially-modulate-primary-motor-cortex-excitability
#5
Ryoki Sasaki, Shinichi Kotan, Masaki Nakagawa, Shota Miyaguchi, Sho Kojima, Kei Saito, Yasuto Inukai, Hideaki Onishi
Modulation of cortical excitability by sensory inputs is a critical component of sensorimotor integration. Sensory afferents, including muscle and joint afferents, to somatosensory cortex (S1) modulate primary motor cortex (M1) excitability, but the effects of muscle and joint afferents specifically activated by muscle contraction are unknown. We compared motor evoked potentials (MEPs) following median nerve stimulation (MNS) above and below the contraction threshold based on the persistence of M-waves. Peripheral nerve electrical stimulation (PES) conditions, including right MNS at the wrist at 110% motor threshold (MT; 110% MNS condition), right MNS at the index finger (sensory digit nerve stimulation [DNS]) with stimulus intensity approximately 110% MNS (DNS condition), and right MNS at the wrist at 90% MT (90% MNS condition) were applied...
2017: Frontiers in Human Neuroscience
https://www.readbyqxmd.com/read/28375329/study-of-a-weak-scattering-model-in-aero-optic-simulations-and-its-computation
#6
Lingfei Xu, Zhichao Zhou, Tianrong Ren
Atmosphere optic statistical modeling cannot be used to analyze aerodynamic flow laser beam propagation characteristics without accounting for the density spectrum of the refractive index. However, numerical simulations support the analysis of these characteristics through ray tracing, random-phase-screen diffraction modeling, and Maxwell-equation-based scattering modeling. We analyzed the laser-beam propagation in aero-optics using the Maxwell-equation-based scattering model. Three approaches offer solutions to Maxwell's equations in statistically inhomogeneous media: high-order numerical differentiations with paraxial approximation and Runge-Kutta methods, Born-approximated scattering potential integral equations, and Rytov-approximated scattering potential integral equations...
April 1, 2017: Journal of the Optical Society of America. A, Optics, Image Science, and Vision
https://www.readbyqxmd.com/read/28355258/the-value-of-gd-eob-dtpa-enhanced-mr-imaging-in-characterizing-cirrhotic-nodules-with-atypical-enhancement-on-gd-dtpa-enhanced-mr-images
#7
Yi-Chun Wang, Chen-Te Chou, Ching-Po Lin, Yao-Li Chen, Yung-Fang Chen, Ran-Chou Chen
PURPOSE: To evaluate the utility of Gd-EOB-DTPA-enhanced magnetic resonance imaging (MRI) in characterizing atypically enhanced cirrhotic nodules detected on conventional Gd-DTPA-enhanced MR images. MATERIALS AND METHODS: We enrolled 61 consecutive patients with 88 atypical nodules seen on conventional Gd-DTPA-enhanced MR images who underwent Gd-EOB-DTPA-enhanced MRI within a 3-month period. Using a reference standard, we determined that 58 of the nodules were hepatocellular carcinoma (HCC) and 30 were dysplastic nodules (DNs)...
2017: PloS One
https://www.readbyqxmd.com/read/28349731/serum-neuron-specific-enolase-as-an-early-predictor-of-delayed-neuropsychiatric-sequelae-in-patients-with-acute-carbon-monoxide-poisoning
#8
Y S Cha, H Kim, H H Do, H I Kim, O H Kim, K-C Cha, K H Lee, S O Hwang
Delayed onset of neuropsychiatric symptoms after apparent recovery from acute carbon monoxide (CO) poisoning has been described as delayed neuropsychiatric sequelae (DNS). To date, there have been no studies on the utility of serum neuron-specific enolase (NSE), a marker of neuronal cell damage, as a predictive marker of DNS in acute CO poisoning. This retrospective observational study was performed on adult patients with acute CO poisoning consecutively treated over a 9-month period. Serum NSE was measured after emergency department arrival, and patients were divided into two groups...
January 1, 2017: Human & Experimental Toxicology
https://www.readbyqxmd.com/read/28322219/two-dimensional-turbulence-in-symmetric-binary-fluid-mixtures-coarsening-arrest-by-the-inverse-cascade
#9
Prasad Perlekar, Nairita Pal, Rahul Pandit
We study two-dimensional (2D) binary-fluid turbulence by carrying out an extensive direct numerical simulation (DNS) of the forced, statistically steady turbulence in the coupled Cahn-Hilliard and Navier-Stokes equations. In the absence of any coupling, we choose parameters that lead (a) to spinodal decomposition and domain growth, which is characterized by the spatiotemporal evolution of the Cahn-Hilliard order parameter ϕ, and (b) the formation of an inverse-energy-cascade regime in the energy spectrum E(k), in which energy cascades towards wave numbers k that are smaller than the energy-injection scale kin j in the turbulent fluid...
March 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28297886/reynolds-number-scaling-of-velocity-increments-in-isotropic-turbulence
#10
Kartik P Iyer, Katepalli R Sreenivasan, P K Yeung
Using the largest database of isotropic turbulence available to date, generated by the direct numerical simulation (DNS) of the Navier-Stokes equations on an 8192^{3} periodic box, we show that the longitudinal and transverse velocity increments scale identically in the inertial range. By examining the DNS data at several Reynolds numbers, we infer that the contradictory results of the past on the inertial-range universality are artifacts of low Reynolds number and residual anisotropy. We further show that both longitudinal and transverse velocity increments scale on locally averaged dissipation rate, just as postulated by Kolmogorov's refined similarity hypothesis, and that, in isotropic turbulence, a single independent scaling adequately describes fluid turbulence in the inertial range...
February 2017: Physical Review. E
https://www.readbyqxmd.com/read/28273984/study-of-risk-factors-for-development-of-voice-disorders-and-its-impact-on-the-quality-of-life-of-school-teachers-in-mangalore-india
#11
Arati Alva, Megna Machado, Kiran Bhojwani, Suja Sreedharan
INTRODUCTION: School teachers are most prone to the development and detrimental effects of voice disorders as a consequence of their work. The risk factors for development of dysphonia in teachers are multifactorial. AIM: The primary aim of our study was to investigate the various risk factors that influence the onset and progression of voice disorders in school teachers in the Indian context. We wanted to assess the effect of voice problems on the physical, psychosocial and functional aspect of a teacher's life...
January 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28262612/gold-dendrites-co-deposited-with-m13-virus-as-a-biosensor-platform-for-nitrite-ions
#12
Yeji Seo, Shanmugam Manivannan, Inhak Kang, Seung-Wuk Lee, Kyuwon Kim
We developed a biosensor for nitrite ion on an electrode surface modified with M13 viruses and gold nanostructures. Gold dendritic nanostructures (Au-DNs) are electrochemically co-deposited from 4E peptides engineered M13 virus (M134E) mixed electrolyte on to the ITO electrode. The M134E could specifically nucleate Au precursor (Gold (III) chloride), which enable the efficient growth of dendritic nanostructures, whereas such dendritic structures were not obtained in the presence of wild-type and Y3E peptides engineered M13 viruses...
February 24, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28250248/comparative-effects-of-denosumab-or-bisphosphonate-treatment-on-bone-mineral-density-and-calcium-metabolism-in-postmenopausal-women
#13
A Augoulea, E Tsakonas, I Triantafyllopoulos, D Rizos, E Armeni, N Tsoltos, S Tournis, E Deligeoroglou, A Antoniou, I Lambrinoudaki
OBJECTIVES: To clarify potential differences between denosumab (DNS) and bisphosphonates (BIS) in terms of bone density and bone metabolism, in a sample of postmenopausal women. METHODS: A total of 113 postmenopausal women aged 53-66 years were treated with either DNS or BIS for 12 months. Bone densitometry and laboratory tests were compared between baseline and follow-up. RESULTS: Femoral neck BMD increased in both treatment-arms (FN-BMD, DNS: 0...
March 1, 2017: Journal of Musculoskeletal & Neuronal Interactions
https://www.readbyqxmd.com/read/28208245/preparation-of-yellow-green-emissive-carbon-dots-and-their-application-in-constructing-a-fluorescent-turn-on-nanoprobe-for-imaging-of-selenol-in-living-cells
#14
Qin Wang, Shengrui Zhang, Yaogang Zhong, Xiao-Feng Yang, Zheng Li, Hua Li
Selenocysteine (Sec) carries out the majority of the functions of the various Se-containing species in vivo. Thus, it is of great importance to develop sensitive and selective assays to detect Sec. Herein, a carbon-dot-based fluorescent turn-on probe for highly selective detection of selenol in living cells is presented. The highly photoluminescent carbon dots that emit yellow-green fluorescence (Y-G-CDs; λmax = 520 nm in water) were prepared by using m-aminophenol as carbon precursor through a facile solvothermal method...
February 7, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28184988/cloning-evaluation-and-high-level-expression-of-a-thermo-alkaline-pectate-lyase-from-alkaliphilic-bacillus-clausii-with-potential-in-ramie-degumming
#15
Cheng Zhou, Yanfen Xue, Yanhe Ma
Alkaline pectate lyases (Pels) have potential application in bioscouring of the textile industry. In this study, a thermo-alkaline Pel (BacPelA) gene from an alkaliphilic Bacillus clausii strain was cloned and overexpressed in Escherichia coli. The mature BacPelA exhibited maximum activity at pH 10.5 and 70 °C and showed high cleavage capability on methylated pectins. BacPelA showed the highest specific activity of 936.2 U mg(-1) on ≥85% methylated pectin and 675.5 U mg(-1) on standard substrate polygalacturonic acid (PGA) upon evaluation of the absorbance at 235 nm (A235)...
February 9, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28165743/design-and-synthesis-of-novel-and-selective-phosphodiesterase-2-pde2a-inhibitors-for-the-treatment-of-memory-disorders
#16
Laurent Gomez, Mark Eben Massari, Troy Vickers, Graeme Freestone, William Vernier, Kiev Ly, Rui Xu, Margaret McCarrick, Tami Marrone, Markus Metz, Yingzhou G Yan, Zachary W Yoder, Robert Lemus, Nicola J Broadbent, Richard Barido, Noelle Warren, Kara Schmelzer, David Neul, Dong Lee, Carsten B Andersen, Kristen Sebring, Kathleen Aertgeerts, Xianbo Zhou, Ali Tabatabaei, Marco Peters, J Guy Breitenbucher
A series of potent and selective [1,2,4]triazolo[1,5-a]pyrimidine PDE2a inhibitors is reported. The design and improvement of the binding properties of this series was achieved using X-ray crystal structures in conjunction with careful analysis of electronic and structural requirements for the PDE2a enzyme. One of the lead compounds, compound 27 (DNS-8254), was identified as a potent and highly selective PDE2a enzyme inhibitor with favorable rat pharmacokinetic properties. Interestingly, the increased potency of compound 27 was facilitated by the formation of a halogen bond with the oxygen of Tyr827 present in the PDE2a active site...
February 21, 2017: Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/28160709/normal-viscous-force-of-pendular-liquid-bridge-between-two-relatively-moving-particles
#17
Kimiaki Washino, Ei L Chan, Taku Matsumoto, Seiji Hashino, Takuya Tsuji, Toshitsugu Tanaka
In this work, Direct Numerical Simulations (DNS) of a pendular liquid bridge formed between two relatively moving particles are performed to evaluate the normal component of the viscous force exerted on the particles. The viscous force obtained are non-dimensionalised in order to clarify the parameters which can affect the dimensionless force. The DNS results are compared with the viscous force models in literature which are commonly used in DEM simulations. It is found that these models cannot be used with large inter-particle separation distance...
May 15, 2017: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/28114366/progressive-enrichment-of-stemness-features-and-tumor-stromal-alterations-in-multistep-hepatocarcinogenesis
#18
Jeong Eun Yoo, Young-Joo Kim, Hyungjin Rhee, Haeryoung Kim, Ei Yong Ahn, Jin Sub Choi, Massimo Roncalli, Young Nyun Park
Cancer stem cells (CSCs), a subset of tumor cells, contribute to an aggressive biological behavior, which is also affected by the tumor stroma. Despite the role of CSCs and the tumor stroma in hepatocellular carcinoma (HCC), features of stemness have not yet been studied in relation to tumor stromal alterations in multistep hepatocarcinogenesis. We investigated the expression status of stemness markers and tumor stromal changes in B viral carcinogenesis, which is the main etiology of HCC in Asia. Stemness features of tumoral hepatocytes (EpCAM, K19, Oct3/4, c-KIT, c-MET, and CD133), and tumor stromal cells expressing α-smooth muscle actin (α-SMA), CD68, CD163, and IL-6 were analyzed in 36 low grade dysplastic nodules (DNs), 48 high grade DNs, 30 early HCCs (eHCCs), and 51 progressed HCCs (pHCCs) by immunohistochemistry or real-time PCR...
2017: PloS One
https://www.readbyqxmd.com/read/28111737/expression-of-dna-methylation-related-proteins-in-invasive-lobular-carcinoma-of-breast-comparison-to-invasive-ductal-carcinoma
#19
Yoon Jin Cha, Hye Min Kim, Ja Seung Koo
PURPOSE: We aimed to compare the expression of DNA methylation-related proteins in invasive lobular carcinoma (ILC) of breast with those of invasive ductal carcinoma (IDC) of breast and to assess its potential clinical application. METHODS: Immunohistochemical staining of DNS methylation-related proteins (5-meC, DNMT1, DNMT3B and ISL-1) was applied to tissue microarrays generated from 108 ILCs and 203 IDCs. Protein expression and its correlation with clinicopatholgic variables were statistically analyzed...
January 23, 2017: Histology and Histopathology
https://www.readbyqxmd.com/read/28078553/expression-of-apoptosis-regulatory-genes-in-the-hippocampus-of-rat-neonates-born-to-mothers-with-diabetes
#20
Hossein Haghir, Javad Hami, Nassim Lotfi, Mostafa Peyvandi, Simagol Ghasemi, Mehran Hosseini
Diabetes during pregnancy impairs the development of the central nervous system (CNS) and causes cognitive and behavioral abnormalities in offspring. However, the exact mechanism by which the maternal diabetes affects the development of the brain remains to be elucidated. The aim of the present study was to investigate the effects of maternal diabetes in pregnancy on the expression of Bcl-2 and Bax genes and the numerical density of degenerating dark neurons (DNs) in the hippocampus of offspring at the first postnatal two weeks...
April 2017: Metabolic Brain Disease
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