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Wenjun Li, Xiao Zhou, Zhenyan Shi, Yang Liu, Zhantao Liu, Hua Gao
TMG-promoted [3 + 2] organocatalytic 1,3-dipolar cycloaddition reactions of allyl ketones with in situ generated nitrile oxides have been developed. This strategy could generate 3,5-disubstituted isoxazolines in high yields and regioselectivities.
October 10, 2016: Organic & Biomolecular Chemistry
Emily Harrop, Simon Noble, Michelle Edwards, Stephanie Sivell, Barbara Moore, Annmarie Nelson
BACKGROUND: Few studies have explored in depth the experiences of patients with advanced cancer who are participating in clinical investigational medicinal product trials. However, integrated qualitative studies in such trials are needed to enable a broader evaluation of patient experiences in the trial, with important ethical and practical implications for the design and conduct of similar trials and treatment regimes in the future. METHODS: Ten participants were recruited from the control and intervention arms of FRAGMATIC: a non-placebo trial for patients with advanced lung cancer...
2016: Trials
Christopher M Zall, John C Linehan, Aaron M Appel
The copper(I) triphosphine complex LCu(MeCN)PF6 (L = 1,1,1-tris(diphenylphosphinomethyl)ethane), which we recently demonstrated is an active catalyst precursor for hydrogenation of CO2 to formate, reacts with H2 in the presence of a base to form a cationic dicopper hydride, [(LCu)2H]PF6. [(LCu)2H](+) is also an active precursor for catalytic CO2 hydrogenation, with equivalent activity to that of LCu(MeCN)(+), and therefore may be a relevant catalytic intermediate. The thermodynamic hydricity of [(LCu)2H](+) was determined to be 41...
August 10, 2016: Journal of the American Chemical Society
Rafaela Muniz de Queiroz, Rashna Madan, Jeremy Chien, Wagner Barbosa Dias, Chad Slawson
O-GlcNAcylation is a dynamic post-translational modification consisting of the addition of a single N-acetylglucosamine sugar to serine and threonine residues in proteins by the enzyme O-linked β-N-acetylglucosamine transferase (OGT), whereas the enzyme O-GlcNAcase (OGA) removes the modification. In cancer, tumor samples present with altered O-GlcNAcylation; however, changes in O-GlcNAcylation are not consistent between tumor types. Interestingly, the tumor suppressor p53 is modified by O-GlcNAc, and most solid tumors contain mutations in p53 leading to the loss of p53 function...
September 2, 2016: Journal of Biological Chemistry
Radhika Agarwal, Beate Schwer, Stewart Shuman
Luc7 is an essential 261-amino acid protein subunit of the Saccharomyces cerevisiae U1 snRNP. To establish structure-function relations for yeast Luc7, we conducted an in vivo mutational analysis entailing N- and C-terminal truncations and alanine scanning of phylogenetically conserved amino acids, including two putative zinc finger motifs, ZnF1 and ZnF2, and charged amino acids within the ZnF2 module. We identify Luc7-(31-246) as a minimal functional protein and demonstrate that whereas mutations of the CCHH ZnF2 motif are lethal, mutations of the ZnF1 CCCH motif and the charged residues of the ZnF2 modules are not...
September 2016: RNA
Hochan Lee, Jun-Ho Choi, Pramod Kumar Verma, Minhaeng Cho
The IR absorption and time-resolved IR spectroscopy of the OD stretch mode of HDO in water was successfully used to study osmolyte effects on water H-bonding network. Protecting osmolytes such as sorbitol and trimethylglycine (TMG) make the vibrational OD stretch band red-shifted, whereas urea affects the OD band marginally. Furthermore, we recently showed that, even though sorbitol and TMG cause a slow-down of HDO rotation in their aqueous solutions, urea does not induce any change in the rotational relaxation of HDO in aqueous urea solutions even at high concentrations...
July 28, 2016: Journal of Physical Chemistry. A
Rauno Álvaro de Paula Simola, Christian Raeder, Thimo Wiewelhove, Michael Kellmann, Tim Meyer, Mark Pfeiffer, Alexander Ferrauti
The study investigates whether tensiomyography (TMG) is sensitive to differentiate between strength and endurance athletes, and to monitor fatigue after either one week of intensive strength (ST) or endurance (END) training. Fourteen strength (24.1±2.0years) and eleven endurance athletes (25.5±4.8years) performed an intensive training period of 6days of ST or END, respectively. ST and END groups completed specific performance tests as well as TMG measurements of maximal radial deformation of the muscle belly (Dm), deformation time between 10% and 90% Dm (Tc), rate of deformation development until 10% Dm (V10) and 90% Dm (V90) before (baseline), after training period (post1), and after 72h of recovery (post2)...
October 2016: Journal of Electromyography and Kinesiology
Zhen Zhang, Flávia C Costa, Ee Phie Tan, Nathan Bushue, Luciano DiTacchio, Catherine E Costello, Mark E McComb, Stephen A Whelan, Kenneth R Peterson, Chad Slawson
One mode of γ-globin gene silencing involves a GATA-1·FOG-1·Mi2β repressor complex that binds to the -566 GATA site relative to the (A)γ-globin gene cap site. However, the mechanism of how this repressor complex is assembled at the -566 GATA site is unknown. In this study, we demonstrate that the O-linked N-acetylglucosamine (O-GlcNAc) processing enzymes, O-GlcNAc-transferase (OGT) and O-GlcNAcase (OGA), interact with the (A)γ-globin promoter at the -566 GATA repressor site; however, mutation of the GATA site to GAGA significantly reduces OGT and OGA promoter interactions in β-globin locus yeast artificial chromosome (β-YAC) bone marrow cells...
July 22, 2016: Journal of Biological Chemistry
Jianling Wang, Xiaofeng Xiao, Tong Chen, Tingfei Liu, Huaming Tao, Jun He
The glyceride in oil food simulant usually causes serious interferences to target analytes and leads to failure of the normal function of the RP-HPLC column. In this work, a convenient HPLC-UV method for the determination of the total specific migration of nine ultraviolet (UV) absorbers in food simulants was developed based on 1,1,3,3-tetramethylguanidine (TMG) and organic phase anion exchange (OPAE) SPE to efficiently remove glyceride in olive oil simulant. In contrast to the normal ion exchange carried out in an aqueous solution or aqueous phase environment, the OPAE SPE was performed in the organic phase environments, and the time-consuming and challenging extraction of the nine UV absorbers from vegetable oil with aqueous solution could be readily omitted...
June 17, 2016: Journal of Chromatography. A
Xiao Zhou, Xianhong Xu, Zhenyan Shi, Kun Liu, Hua Gao, Wenjun Li
TMG-catalyzed [3 + 2] organocatalytic 1,3-dipolar cycloaddition reactions of β-functionalized ketones with nitrile oxides have been developed. This strategy could generate 3,4,5-trisubstituted isoxazoles in high yields and regioselectivities.
June 21, 2016: Organic & Biomolecular Chemistry
S Azarpeykan, K E Dittmer, E K Gee, J C Marshall, J Wallace, P Elder, E Acke, K G Thompson
The aims of the study were to determine the effect of season and blanketing on vitamin D synthesis in horses and examine the interaction between vitamin D and other analytes involved in calcium homeostasis. Twenty-one healthy horses at pasture were included; 5 were covered with standard horse blankets including neck rugs. Blood samples were collected for 13 mo and analyzed for 25-hydroxyvitamin D2 (25OHD2) and 25-hydroxyvitamin D3 (25OHD3), 1,25-dihydroxyvitamin D (1,25[OH]2D), ionized calcium (iCa), total calcium (tCa), phosphorus (P), total magnesium (tMg), and parathyroid hormone (PTH)...
July 2016: Domestic Animal Endocrinology
John M S Bartlett, Jane Bayani, Andrea Marshall, Janet A Dunn, Amy Campbell, Carrie Cunningham, Monika S Sobol, Peter S Hall, Christopher J Poole, David A Cameron, Helena M Earl, Daniel W Rea, Iain R Macpherson, Peter Canney, Adele Francis, Christopher McCabe, Sarah E Pinder, Luke Hughes-Davies, Andreas Makris, Robert C Stein
BACKGROUND: Previous reports identifying discordance between multiparameter tests at the individual patient level have been largely attributed to methodological shortcomings of multiple in silico studies. Comparisons between tests, when performed using actual diagnostic assays, have been predicted to demonstrate high degrees of concordance. OPTIMA prelim compared predicted risk stratification and subtype classification of different multiparameter tests performed directly on the same population...
September 2016: Journal of the National Cancer Institute
Edmund Fitzgerald O'Connor, Warren Matthew Rozen, Muhammad Chowdhry, Nakul Gamanlal Patel, Whitney T H Chow, Matthew Griffiths, Venkat V Ramakrishnan
BACKGROUND: Venous couplers are ubiquitous around the world and are a useful tool for the reconstructive microsurgeon. A systematic review of coupler performance studies demonstrated a thrombosis rate range of 0% to 3%, whilst the average time of using the device is 5 minutes. There is sparse published data on cost analysis and the impact of operator experience on the anastomotic coupler device success. Improvements in outcomes other than time benefits have also not been shown. This study aims to address these deficiencies in the literature...
April 2016: Gland Surgery
William P Miller, Maria L Mihailescu, Chen Yang, Alistair J Barber, Scot R Kimball, Leonard S Jefferson, Michael D Dennis
PURPOSE: The translational repressor 4E-BP1 interacts with the mRNA cap-binding protein eIF4E and thereby promotes cap-independent translation of mRNAs encoding proteins that contribute to diabetic retinopathy. Interaction of 4E-BP1 with eIF4E is enhanced in the retina of diabetic rodents, at least in part, as a result of elevated 4E-BP1 protein expression. In the present study, we examined the role of 4E-BP1 in diabetes-induced visual dysfunction, as well as the mechanism whereby hyperglycemia promotes 4E-BP1 expression...
March 2016: Investigative Ophthalmology & Visual Science
David Lascorz, Victoria López, Carmen Pinedo, Joan Trujols, Joan Vegué, Víctor Pérez
INTRODUCTION: People with severe mental disorder have significant difficulties in everyday life that involve the need for continued support. These needs are not easily measurable with the currently available tools. Therefore, a multidimensional scale that assesses the different levels of need for care is proposed, including a study of its psychometric properties. METHOD: One-hundred and thirty-nine patients (58% men) with a severe mental disorder were assessed using the Required Care Levels for People with Severe Mental Disorder Assessment Scale (ENAR-TMG), the Camberwell Assessment of Need scale, and the Health of the Nation Outcome Scales...
March 8, 2016: Revista de Psiquiatrí́a y Salud Mental
Silvia Heiss, Angelika Hörmann, Christopher Tauer, Margot Sonnleitner, Esther Egger, Reingard Grabherr, Stefan Heinl
BACKGROUND: Engineering lactic acid bacteria (LAB) is of growing importance for food and feed industry as well as for in vivo vaccination or the production of recombinant proteins in food grade organisms. Often, expression of a transgene is only desired at a certain time point or period, e.g. to minimize the metabolic burden for the host cell or to control the expression time span. For this purpose, inducible expression systems are preferred, though cost and availability of the inducing agent must be feasible...
2016: Microbial Cell Factories
Jin Xu, Xiaoling Zha, Yumei Wu, Qingping Ke, Weifang Yu
SO2 capacity of the obtained TMG-AlPO-5/cordierite honeycomb ceramic (CHC) adsorbent was measured to be 1.13 mol per mol TMG. More importantly, compared with literature reported supported ionic liquids, it is featured by a significantly improved adsorption rate (t0.9 reduced from >30 min to ∼0.1 min) and negligible pressure drop.
May 11, 2016: Chemical Communications: Chem Comm
Roberto Seijas, Eduard Alentorn-Geli, Pedro Álvarez-Díaz, Miguel Marín, Oscar Ares, Andrea Sallent, Xavier Cuscó, Ramón Cugat
INTRODUCTION: the aim of the present study is to evaluate the mechanical and contractile properties of the gluteus maximus (GM) muscle in patients with femoroacetabular impingement (FAI). Our hypothesis is that the clinical observation of GM pain would be evidenced by tensiomyographic impairment in muscle function. MATERIALS AND METHODS: A prospective, cross-sectional, intra-group comparative study was conducted to assess the neuromuscular changes of lower extremity muscles in patients with FAI...
June 2016: Archives of Orthopaedic and Trauma Surgery
S Azarpeykan, K E Dittmer, E K Gee, J C Marshall, P Elder, E Acke, K G Thompson
A study was conducted to determine the circadian rhythms and trends of vitamin D metabolites including 25-hydroxyvitamin D3 , 25-hydroxyvitamin D2 , 1,25-dihydroxyvitamin D and parathyroid hormone, in addition to serum calcium, phosphorus and magnesium concentrations in horses over 48 h on the shortest and longest days of the year in 2013. Five healthy adult horses (Equus caballus) were on a constant pasture feeding regimen, and blood samples were collected from each horse every 3 h over a 48-h period, starting at 07:00 PM on day one and finishing at 07:00 PM on day three, for the measurement of calciotropic hormones and electrolytes...
February 3, 2016: Journal of Animal Physiology and Animal Nutrition
Emily Harrop, John Kelly, Gareth Griffiths, Angela Casbard, Annmarie Nelson
BACKGROUND: Surgical trials have typically experienced recruitment difficulties when compared with other types of oncology trials. Qualitative studies have an important role to play in exploring reasons for low recruitment, although to date few such studies have been carried out that are embedded in surgical trials. The BOLERO trial (Bladder cancer: Open versus Lapararoscopic or RObotic cystectomy) is a study to determine the feasibility of randomisation to open versus laparoscopic access/robotic cystectomy in patients with bladder cancer...
2016: Trials
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