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Bacteria sugar utilization

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https://www.readbyqxmd.com/read/27924829/diverse-specificity-of-cellulosome-attachment-to-the-bacterial-cell-surface
#1
Joana L A Brás, Benedita A Pinheiro, Kate Cameron, Fiona Cuskin, Aldino Viegas, Shabir Najmudin, Pedro Bule, Virginia M R Pires, Maria João Romão, Edward A Bayer, Holly L Spencer, Steven Smith, Harry J Gilbert, Victor D Alves, Ana Luísa Carvalho, Carlos M G A Fontes
During the course of evolution, the cellulosome, one of Nature's most intricate multi-enzyme complexes, has been continuously fine-tuned to efficiently deconstruct recalcitrant carbohydrates. To facilitate the uptake of released sugars, anaerobic bacteria use highly ordered protein-protein interactions to recruit these nanomachines to the cell surface. Dockerin modules located within a non-catalytic macromolecular scaffold, whose primary role is to assemble cellulosomal enzymatic subunits, bind cohesin modules of cell envelope proteins, thereby anchoring the cellulosome onto the bacterial cell...
December 7, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27915410/the-properties-and-potential-metabolic-role-of-glucokinase-in-halotolerant-obligate-methanotroph-methylomicrobium-alcaliphilum-20z
#2
Ildar I Mustakhimov, Olga N Rozova, Natalia P Solntseva, Valentina N Khmelenina, Alexander S Reshetnikov, Yuri A Trotsenko
Aerobic bacteria utilizing methane as the carbon and energy source do not use sugars as growth substrates but possess the gene coding for glucokinase (Glk), an enzyme converting glucose into glucose 6-phosphate. Here we demonstrate the functionality and properties of Glk from an obligate methanotroph Methylomicrobium alcaliphilum 20Z. The recombinant Glk obtained by heterologous expression in Escherichia coli was found to be close in biochemical properties to other prokaryotic Glks. The homodimeric enzyme (2 × 35 kDa) catalyzed ATP-dependent phosphorylation of glucose and glucosamine with nearly equal activity, being inhibited by ADP (K i = 2...
December 3, 2016: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/27902282/defining-the-taxonomic-status-of-described-subdivision-3-acidobacteria-the-proposal-of-bryobacteraceae-fam-nov
#3
Svetlana N Dedysh, Irina S Kulichevskaya, Katharina J Huber, Jörg Overmann
Acidobacteria represent one of the highly diverse but poorly characterized phylogenetic groups of the domain Bacteria. The taxonomically described acidobacteria belong to 27 genera and 49 species, which represent subdivisions 1, 3, 4, 6, 8, 10 and 23 of this phylum. However, the corresponding family ranks have been defined only for some of these characterized microorganisms. Here, we suggest the establishment of a novel family, Bryobacteraceae fam. nov., to accommodate taxonomically described members of subdivision 3 Acidobacteria...
November 24, 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27872964/toward-sustainable-amino-acid-production
#4
Yoshihiro Usuda, Yoshihiko Hara, Hiroyuki Kojima
Because the global amino acid production industry has been growing steadily and is expected to grow even more in the future, efficient production by fermentation is of great importance from economic and sustainability viewpoints. Many systems biology technologies, such as genome breeding, omics analysis, metabolic flux analysis, and metabolic simulation, have been employed for the improvement of amino acid-producing strains of bacteria. Synthetic biological approaches have recently been applied to strain development...
November 22, 2016: Advances in Biochemical Engineering/biotechnology
https://www.readbyqxmd.com/read/27836849/identification-of-a-new-phosphatase-enzyme-potentially-involved-in-sugar-phosphate-stress-response-in-pseudomonas-fluorescens
#5
Susan Maleki, Radka Hrudikova, Sergey B Zotchev, Helga Ertesvåg
: The alginate-producing bacterium Pseudomonas fluorescens utilizes the Entner-Doudoroff (ED) and pentose phosphate (PP) pathways to metabolize fructose, since the upper part of its Embden-Meyerhof-Parnas pathway is defective. Our previous study indicated that perturbation of the central carbon metabolism by diminishing glucose-6-phosphate dehydrogenase activity could lead to sugar-phosphate stress when P. fluorescens was cultivated on fructose. In the present study, we demonstrate that PFLU2693, annotated as a haloacid dehalogenase-like enzyme, is a new sugar-phosphate phosphatase now designated Spp, which is able to dephosphorylate a range of phosphate substrates including glucose 6-phosphate and fructose 6-phosphate in vitro The effect of spp overexpression on growth and alginate production was investigated using both wild type and several mutant strains...
November 11, 2016: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/27815279/comparative-proteogenomics-and-metabolic-profiling-identify-an-inducible-operon-involved-in-inulin-utilization-in-lactobacillus-plantarum-strains
#6
Nirunya Buntin, Tipparat Hongpattarakere, Jarmo Ritari, François P Douillard, Lars Paulin, Sjef Boeren, Sudarshan A Shetty, Willem M de Vos
: The draft genome of L. plantarum isolated from Asian fermented foods, infant feces and shrimp intestines were sequenced and compared to those of well-studied strains. Among twenty-eight strains of L. plantarum, the variation of genomic features involved in the ecological adaptation were elucidated. The genome sizes ranged from approximately 3.1 to 3.5 Mb, of which about 2,932 to 3,345 of protein-coding sequences (CDS) were predicted. The food derived isolates contained higher number of carbohydrate metabolism associated genes than those from infant feces...
November 4, 2016: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/27803186/metabolism-of-toxic-sugars-by-strains-of-the-bee-gut-symbiont-gilliamella-apicola
#7
Hao Zheng, Alex Nishida, Waldan K Kwong, Hauke Koch, Philipp Engel, Margaret I Steele, Nancy A Moran
: Social bees collect carbohydrate-rich food to support their colonies, and yet, certain carbohydrates present in their diet or produced through the breakdown of pollen are toxic to bees. The gut microbiota of social bees is dominated by a few core bacterial species, including the Gram-negative species Gilliamella apicola We isolated 42 strains of G. apicola from guts of honey bees and bumble bees and sequenced their genomes. All of the G. apicola strains share high 16S rRNA gene similarity, but they vary extensively in gene repertoires related to carbohydrate metabolism...
November 1, 2016: MBio
https://www.readbyqxmd.com/read/27800149/the-effects-of-sugars-on-the-biofilm-formation-of-escherichia-coli-185p-on-stainless-steel-and-polyethylene-terephthalate-surfaces-in-a-laboratory-model
#8
Mahdi Khangholi, Ailar Jamalli
BACKGROUND: Bacteria utilize various methods in order to live in protection from adverse environmental conditions. One such method involves biofilm formation; however, this formation is dependent on many factors. The type and concentration of substances such as sugars that are present in an environment can be effective facilitators of biofilm formation. METHODS: First, the physico-chemical properties of the bacteria and the target surface were studied via the MATS and contact angle measurement methods...
September 2016: Jundishapur Journal of Microbiology
https://www.readbyqxmd.com/read/27766829/development-of-rare-bacterial-monosaccharide-analogs-for-metabolic-glycan-labeling-in-pathogenic-bacteria
#9
Emily L Clark, Madhu Emmadi, Katharine L Krupp, Ananda R Podilapu, Jennifer D Helble, Suvarn S Kulkarni, Danielle H Dube
Bacterial glycans contain rare, exclusively bacterial monosaccharides that are frequently linked to pathogenesis and essentially absent from human cells. Therefore, bacterial glycans are intriguing molecular targets. However, systematic discovery of bacterial glycoproteins is hampered by the presence of rare deoxy amino sugars, which are refractory to traditional glycan-binding reagents. Thus, the development of chemical tools that label bacterial glycans is a crucial step toward discovering and targeting these biomolecules...
October 21, 2016: ACS Chemical Biology
https://www.readbyqxmd.com/read/27754312/new-insights-into-various-production-characteristics-of-streptococcus-thermophilus-strains
#10
Yanhua Cui, Tingting Xu, Xiaojun Qu, Tong Hu, Xu Jiang, Chunyu Zhao
Streptococcus thermophilus is one of the most valuable homo-fermentative lactic acid bacteria, which, for a long time, has been widely used as a starter for the production of fermented dairy products. The key production characteristics of S. thermophilus, for example the production of extracellular polysaccharide, proteolytic enzymes and flavor substances as well as acidifying capacity etc., have an important effect on the quality of dairy products. The acidification capacity of the strains determines the manufacturing time and quality of dairy products...
October 12, 2016: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/27734757/agrowaste-to-vanillin-conversion-by-a-natural-pediococcus-acidilactici-strain-bd16
#11
Debkumar Chakraborty, Baljinder Kaur, Karthikeyan Obulisamy, Ammaiyappan Selvam, Jonathan W C Wong
Owing to its flavoring, antimicrobial, antioxidant and anticarcinogenic nature, vanillin is widely used in foods, beverages, perfumes and pharmaceutical products. Ferulic acid (FA) is an important precursor of vanillin which is abundant in cereals like maize, rice and wheat and sugar beet. A major drawback of microbial vanillin production from FA is the degradation and biotransformation of toxic vanillin to other phenolic derivatives. The present study is undertaken to explore microbial vanillin production from FA precursor rice bran by employing vanillin-resistant Pediococcus acidilactici BD16, a natural lactic acid bacteria isolate...
October 13, 2016: Environmental Technology
https://www.readbyqxmd.com/read/27734121/phylogenetic-signal-in-phenotypic-traits-related-to-carbon-source-assimilation-and-chemical-sensitivity-in-acinetobacter-species
#12
Ado Van Assche, Sergio Álvarez-Pérez, Anna de Breij, Joseph De Brabanter, Kris A Willems, Lenie Dijkshoorn, Bart Lievens
A common belief is that the phylogeny of bacteria may reflect molecular functions and phenotypic characteristics, pointing towards phylogenetic conservatism of traits. Here, we tested this hypothesis for a large set of Acinetobacter strains. Members of the genus Acinetobacter are widespread in nature, demonstrate a high metabolic diversity and are resistant to several environmental stressors. Notably, some species are known to cause opportunistic human infections. A total of 133 strains belonging to 33 species with validly published names, two genomic species and species of an as-yet unknown taxonomic status were analyzed using the GENIII technology of Biolog, which allows high-throughput phenotyping...
October 12, 2016: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/27664739/organic-acid-mediated-repression-of-sugar-utilization-in-rhizobia
#13
REVIEW
Bhagya Iyer, Mahendrapal Singh Rajput, Rahul Jog, Ekta Joshi, Krishna Bharwad, Shalini Rajkumar
Rhizobia are a class of symbiotic diazotrophic bacteria which utilize C4 acids in preference to sugars and the sugar utilization is repressed as long as C4 acids are present. This can be manifested as a diauxie when rhizobia are grown in the presence of a sugar and a C4 acid together. Succinate, a C4 acid is known to repress utilization of sugars, sugar alcohols, hydrocarbons, etc by a mechanism termed as Succinate Mediated Catabolite Repression (SMCR). Mechanism of catabolite repression determines the hierarchy of carbon source utilization in bacteria...
November 2016: Microbiological Research
https://www.readbyqxmd.com/read/27590817/glycosulfatase-encoding-gene-cluster-in-bifidobacterium-breve-ucc2003
#14
Muireann Egan, Hao Jiang, Mary O'Connell Motherway, Stefan Oscarson, Douwe van Sinderen
: Bifidobacteria constitute a specific group of commensal bacteria typically found in the gastrointestinal tract (GIT) of humans and other mammals. Bifidobacterium breve strains are numerically prevalent among the gut microbiota of many healthy breastfed infants. In the present study, we investigated glycosulfatase activity in a bacterial isolate from a nursling stool sample, B. breve UCC2003. Two putative sulfatases were identified on the genome of B. breve UCC2003. The sulfated monosaccharide N-acetylglucosamine-6-sulfate (GlcNAc-6-S) was shown to support the growth of B...
November 15, 2016: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/27575333/gene-cloning-and-expression-of-cellulase-of-bacillus-amyloliquefaciens-isolated-from-the-cecum-of-goose
#15
Linghong Sun, Jiangyan Cao, Ying Liu, Junjie Wang, Panpan Guo, Zaigui Wang
A kind of bacteria secreting cellulase and showing probiotic attributes was isolated from the cecum of goose and identified as Bacillus amyloliquefaciens by analysis of 16S rRNA gene sequence and named as B. amyloliquefaciens S1. In vitro assays, the enzymatic activity of the strain was determined by the reducing-sugar method, and the proper culture conditions of producing cellulase and some properties of the cellulase were investigated. The cultural mixture of the bacteria had a high cellulase activity of 1...
August 30, 2016: Animal Biotechnology
https://www.readbyqxmd.com/read/27570278/effect-of-lactobacillus-casei-casei-and-lactobacillus-reuteri-on-acrylamide-formation-in-flat-bread-and-bread-roll
#16
Farnaz Dastmalchi, Seyed Hadi Razavi, Mohammad Faraji, Mohsen Labbafi
The aim of this study was the evaluation of fermentation by lactic acid bacteria (LAB) contains lactobacillus (L.) casei- casei and L. reuteri on acrylamide formation and physicochemical properties of the Iranian flat bread named, Sangak, and Bread roll. Sangak and Bread roll were made with whole and white wheat flour, respectively. Whole-wheat flour had upper content of protein, sugar, ash, fiber, damaged starch and the activity of amylase than the white wheat flour. After 24 h of fermentation, the pH values of the sourdoughs made from whole-wheat flour (3...
March 2016: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/27525041/lignocellulosic-saccharification-by-a-newly-isolated-bacterium-ruminiclostridium-thermocellum-m3-and-cellular-cellulase-activities-for-high-ratio-of-glucose-to-cellobiose
#17
Tao Sheng, Lei Zhao, Ling-Fang Gao, Wen-Zong Liu, Min-Hua Cui, Ze-Chong Guo, Xiao-Dan Ma, Shih-Hsin Ho, Ai-Jie Wang
BACKGROUND: Lignocellulosic biomass is one of earth's most abundant resources, and it has great potential for biofuel production because it is renewable and has carbon-neutral characteristics. Lignocellulose is mainly composed of carbohydrate polymers (cellulose and hemicellulose), which contain approximately 75 % fermentable sugars for biofuel fermentation. However, saccharification by cellulases is always the main bottleneck for commercialization. Compared with the enzyme systems of fungi, bacteria have evolved distinct systems to directly degrade lignocellulose...
2016: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/27481927/unexpected-diversity-of-escherichia-coli-sialate-o-acetyl-esterase-nans
#18
Ariel Rangel, Susan M Steenbergen, Eric R Vimr
UNLABELLED: The sialic acids (N-acylneuraminates) are a group of nine-carbon keto-sugars existing mainly as terminal residues on animal glycoprotein and glycolipid carbohydrate chains. Bacterial commensals and pathogens exploit host sialic acids for nutrition, adhesion, or antirecognition, where N-acetyl- or N-glycolylneuraminic acids are the two predominant chemical forms of sialic acids. Each form may be modified by acetyl esters at carbon position 4, 7, 8, or 9 and by a variety of less-common modifications...
October 15, 2016: Journal of Bacteriology
https://www.readbyqxmd.com/read/27424995/d-mannose-a-promising-support-for-acute-urinary-tract-infections-in-women-a-pilot-study
#19
L Domenici, M Monti, C Bracchi, M Giorgini, V Colagiovanni, L Muzii, P Benedetti Panici
OBJECTIVE: Urinary tract infections still represent a significant bother for women and result in high costs to the health system. D-mannose is a simple sugar; it seems able to hinder bacteria adhesion to the urothelium. The present study aimed to determine whether D-mannose alone is effective in treating acute urinary tract infections in women and its possible utility in the management of recurrences. PATIENTS AND METHODS: This is a pilot study, performed between April 2014 and July 2015 at Department of Gynaecological Obstetrics and Urologic Sciences of "Sapienza" University of Rome...
July 2016: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/27405438/biotransformation-of-chemical-constituents-of-durian-wine-with-simultaneous-alcoholic-fermentation-by-torulaspora-delbrueckii-and-malolactic-fermentation-by-oenococcus-oeni
#20
Yuyun Lu, Jian-Yong Chua, Dejian Huang, Pin-Rou Lee, Shao-Quan Liu
This work represents the first study on the biotransformation of chemical constituents of durian wine via simultaneous alcoholic fermentation (AF) and malolactic fermentation (MLF) with non-Saccharomyces yeast and lactic acid bacteria (LAB), namely, Torulaspora delbrueckii Biodiva and Oenococcus oeni PN4. The presence of PN4 improved the utilization of sugars but did not affect ethanol production. MLF resulted in the significant degradation of malic acid with corresponding increases in pH and lactic acid. The final concentrations of acetic acid (1...
October 2016: Applied Microbiology and Biotechnology
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