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Bacteria sugar utilization

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https://www.readbyqxmd.com/read/28814130/identification-and-characterization-of-a-sulfoglycosidase-from-bifidobacterium-bifidum-implicated-in-mucin-glycan-utilization
#1
Toshihiko Katoh, Takako Maeshibu, Kei-Ichi Kikkawa, Aina Gotoh, Yusuke Tomabechi, Motoharu Nakamura, Wei-Hsiang Liao, Masanori Yamaguchi, Hisashi Ashida, Kenji Yamamoto, Takane Katayama
Human gut symbiont bifidobacteria possess carbohydrate-degrading enzymes that act on the O-linked glycans of intestinal mucins to utilize those carbohydrates as carbon sources. However, our knowledge about mucin type O-glycan degradation by bifidobacteria remains fragmentary, especially regarding how they decompose sulfated glycans, which are abundantly found in mucin sugar-chains. Here, we examined the abilities of several Bifidobacterium strains to degrade a sulfated glycan substrate and identified a 6-sulfo-β-d-N-acetylglucosaminidase, also termed sulfoglycosidase, encoded by bbhII from Bifidobacterium bifidum JCM 7004...
August 17, 2017: Bioscience, Biotechnology, and Biochemistry
https://www.readbyqxmd.com/read/28790984/acidotolerant-bacteria-and-fungi-as-a-sink-of-methanol-derived-carbon-in-a-deciduous-forest-soil
#2
Mareen Morawe, Henrike Hoeke, Dirk K Wissenbach, Guillaume Lentendu, Tesfaye Wubet, Eileen Kröber, Steffen Kolb
Methanol is an abundant atmospheric volatile organic compound that is released from both living and decaying plant material. In forest and other aerated soils, methanol can be consumed by methanol-utilizing microorganisms that constitute a known terrestrial sink. However, the environmental factors that drive the biodiversity of such methanol-utilizers have been hardly resolved. Soil-derived isolates of methanol-utilizers can also often assimilate multicarbon compounds as alternative substrates. Here, we conducted a comparative DNA stable isotope probing experiment under methylotrophic (only [(13)C1]-methanol was supplemented) and combined substrate conditions ([(12)C1]-methanol and alternative multi-carbon [(13)Cu]-substrates were simultaneously supplemented) to (i) identify methanol-utilizing microorganisms of a deciduous forest soil (European beech dominated temperate forest in Germany), (ii) assess their substrate range in the soil environment, and (iii) evaluate their trophic links to other soil microorganisms...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28771501/obliteration-of-bacterial-growth-and-biofilm-through-ros-generation-by-facilely-synthesized-green-silver-nanoparticles
#3
Shariq Qayyum, Mohammad Oves, Asad U Khan
Mangifera indica inflorescence aqueous extract was utilized for production of green AgNPs. Synthesized AgNPs were characterized by UV-vis spectrophotometry, XRD, TEM, FESEM and particles size analyzer. AgNPs showed minimum inhibitory concentrations (MICs) of 8 μg ml-1 and 16 μg ml-1 for Gram negative (K. pneumoniae, P. aeruginosa and E. coli) and Gram positive (S. mutans and S. aureus) strains, respectively which was relatively quite low compared to chemically synthesized silver nanoparticles. AgNPs inhibited 80% and 75% biofilms of E...
2017: PloS One
https://www.readbyqxmd.com/read/28751880/genomic-reconstruction-of-carbohydrate-utilization-capacities-in-microbial-mat-derived-consortia
#4
Semen A Leyn, Yukari Maezato, Margaret F Romine, Dmitry A Rodionov
Two nearly identical unicyanobacterial consortia (UCC) were previously isolated from benthic microbial mats that occur in a heliothermal saline lake in northern Washington State. Carbohydrates are a primary source of carbon and energy for most heterotrophic bacteria. Since CO2 is the only carbon source provided, the cyanobacterium must provide a source of carbon to the heterotrophs. Available genomic sequences for all members of the UCC provide opportunity to investigate the metabolic routes of carbon transfer between autotroph and heterotrophs...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28716923/molecular-mechanism-of-environmental-d-xylose-perception-by-a-xylfii-lyts-complex-in-bacteria
#5
Jianxu Li, Chengyuan Wang, Gaohua Yang, Zhe Sun, Hui Guo, Kai Shao, Yang Gu, Weihong Jiang, Peng Zhang
d-xylose, the main building block of plant biomass, is a pentose sugar that can be used by bacteria as a carbon source for bio-based fuel and chemical production through fermentation. In bacteria, the first step for d-xylose metabolism is signal perception at the membrane. We previously identified a three-component system in Firmicutes bacteria comprising a membrane-associated sensor protein (XylFII), a transmembrane histidine kinase (LytS) for periplasmic d-xylose sensing, and a cytoplasmic response regulator (YesN) that activates the transcription of the target ABC transporter xylFGH genes to promote the uptake of d-xylose...
August 1, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28706879/multiple-substrate-usage-of-coxiella-burnetii-to-feed-a-bipartite-metabolic-network
#6
Ina Häuslein, Franck Cantet, Sarah Reschke, Fan Chen, Matteo Bonazzi, Wolfgang Eisenreich
The human pathogen Coxiella burnetii causes Q-fever and is classified as a category B bio-weapon. Exploiting the development of the axenic growth medium ACCM-2, we have now used (13)C-labeling experiments and isotopolog profiling to investigate the highly diverse metabolic network of C. burnetii. To this aim, C. burnetii RSA 439 NMII was cultured in ACCM-2 containing 5 mM of either [U-(13)C3]serine, [U-(13)C6]glucose, or [U-(13)C3]glycerol until the late-logarithmic phase. GC/MS-based isotopolog profiling of protein-derived amino acids, methanol-soluble polar metabolites, fatty acids, and cell wall components (e...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28687644/genome-scale-model-and-omics-analysis-of-metabolic-capacities-of-akkermansia-muciniphila-reveal-a-preferential-mucin-degrading-lifestyle
#7
Noora Ottman, Mark Davids, Maria Suarez-Diez, Sjef Boeren, Peter J Schaap, Vitor A P Martins Dos Santos, Hauke Smidt, Clara Belzer, Willem M de Vos
The composition and activity of the microbiota in the human gastrointestinal tract is primarily shaped by nutrients derived either from food or the host. Bacteria colonizing the mucus layer have evolved to use mucin as a carbon and energy source. One of the members of the mucosa-associated microbiota is Akkermansia muciniphila, which is capable of producing an extensive repertoire of mucin-degrading enzymes. To further study substrate utilization abilities of A. muciniphila, we constructed a genome-scale metabolic model to test amino acid auxotrophy, vitamin biosynthesis, and sugar degrading capacities...
July 7, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28646159/yvck-a-protein-required-for-cell-wall-integrity-and-optimal-carbon-source-utilization-binds-uridine-diphosphate-sugars
#8
Elodie Foulquier, Anne Galinier
In Bacillus subtilis, Listeria monocytogenes and in two Mycobacteria, it was previously shown that yvcK is a gene required for normal cell shape, for optimal carbon source utilization and for virulence of pathogenic bacteria. Here we report that the B. subtilis protein YvcK binds to Uridine diphosphate-sugars like Uridine diphosphate-Glucose (UDP-Glc) and Uridine diphosphate-N-acetylglucosamine (UDP-GlcNAc) in vitro. Using the crystal structure of Bacillus halodurans YvcK, we identified residues involved in this interaction...
June 23, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28634232/the-phosphocarrier-protein-hpr-of-the-bacterial-phosphotransferase-system-globally-regulates-energy-metabolism-by-directly-interacting-with-multiple-enzymes-in-escherichia-coli
#9
Irina A Rodionova, Zhongge Zhang, Jitender Mehla, Norman Goodacre, Mohan Babu, Andrew Emili, Peter Uetz, Milton H Saier
The histidine-phosphorylatable phosphocarrier protein HPr, is an essential component of the sugar-transporting phosphotransferase system (PTS) in many bacteria. Recent interactome findings suggested that HPr interacts with several carbohydrate-metabolizing enzymes, but whether HPr plays a regulatory role was unclear. Here, we provide evidence that HPr interacts with a large number of proteins in Escherichia coli. We demonstrate HPr-dependent allosteric regulation of the activities of pyruvate kinase (PykF, but not PykA), phosphofructokinase (PfkB, but not PfkA), glucosamine 6-phosphate deaminase (NagB) and adenylate kinase (Adk)...
June 20, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28580630/the-midgut-microbiota-plays-an-essential-role-in-sand-fly-vector-competence-for-leishmania-major
#10
Isabelle Louradour, Carolina Cunha Monteiro, Ehud Inbar, Kashinath Ghosh, Richard Merkhofer, Phillip Lawyer, Andrea Paun, Margery Smelkinson, Nagila Secundino, Michael Lewis, Dinesh Erram, Ludek Zurek, David Sacks
For many arthropod vectors, the diverse bacteria and fungi that inhabit the gut can negatively impact pathogen colonization. Our attempts to exploit antibiotic treatment of colonized Phlebotomus duboscqi sand flies in order to improve their vector competency for Leishmania major resulted instead in flies that were refractory to the development of transmissible infections due to the inability of the parasite to survive and to colonize the anterior midgut with infective, metacyclic stage promastigotes. The parasite survival and development defect could be overcome by feeding the flies on different symbiont bacteria but not by feeding them on bacterial supernatants or replete medium...
June 5, 2017: Cellular Microbiology
https://www.readbyqxmd.com/read/28572960/production-of-organic-flavor-compounds-by-dominant-lactic-acid-bacteria-and-yeasts-from-obushera-a-traditional-sorghum-malt-fermented-beverage
#11
Ivan M Mukisa, Yusuf B Byaruhanga, Charles M B K Muyanja, Thor Langsrud, Judith A Narvhus
Single and mixed starter cultures of lactic acid bacteria (LAB): Weissella confusa MNC20, Lactobacillus plantarum MNC21, Lactococcus lactis MNC24 and Lactobacillus fermentum MNC34 and yeasts: Issatchenkia orientalis MNC20Y and Saccharomyces cerevisiae MNC21Y were used to produce Obushera, a fermented sorghum beverage. Microbial counts, pH, sugars, organic acids, and volatile compounds in starter culture and spontaneous fermentations were monitored during 48 hrs. Maximum counts of LAB (8.4-9.4 log cfu g(-1)) and yeasts (7...
May 2017: Food Science & Nutrition
https://www.readbyqxmd.com/read/28475235/quantitative-proteome-profiles-help-reveal-efficient-xylose-utilization-mechanisms-in-solventogenic-clostridium-sp-strain-boh3
#12
Anindya Basu, Fengxue Xin, Teck Kwang Lim, Qingsong Lin, Kun-Lin Yang, Jianzhong He
Development of sustainable biobutanol production platforms from lignocellulosic materials is impeded by inefficient five carbon sugar uptake by solventogenic bacteria. The recently isolated Clostridium sp. strain BOH3 is particularly advantaged in this regard as it serves as a model organism which can simultaneously utilize both glucose and xylose for high butanol (>15 g/L) production. Strain BOH3 was, therefore, investigated for its metabolic mechanisms for efficient five carbon sugar uptake using a quantitative proteomics based approach...
September 2017: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/28468624/direct-hydrogen-production-from-dilute-acid-pretreated-sugarcane-bagasse-hydrolysate-using-the-newly-isolated-thermoanaerobacterium-thermosaccharolyticum-mj1
#13
Bin-Bin Hu, Ming-Jun Zhu
BACKGROUND: Energy shortage and environmental pollution are two severe global problems, and biological hydrogen production from lignocellulose shows great potential as a promising alternative biofuel to replace the fossil fuels. Currently, most studies on hydrogen production from lignocellulose concentrate on cellulolytic microbe, pretreatment method, process optimization and development of new raw materials. Due to no effective approaches to relieve the inhibiting effect of inhibitors, the acid pretreated lignocellulose hydrolysate was directly discarded and caused environmental problems, suggesting that isolation of inhibitor-tolerant strains may facilitate the utilization of acid pretreated lignocellulose hydrolysate...
May 3, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28465215/glycogen-synthase-from-the-parabasalian-parasite-trichomonas-vaginalis-an-unusual-member-of-the-starch-glycogen-synthase-family
#14
Wayne A Wilson, Prajakta Pradhan, Nayasha Madhan, Galen C Gist, Andrew Brittingham
Trichomonas vaginalis, a parasitic protist, is the causative agent of the common sexually-transmitted infection trichomoniasis. The organism has long been known to synthesize substantial glycogen as a storage polysaccharide, presumably mobilizing this compound during periods of carbohydrate limitation, such as might be encountered during transmission between hosts. However, little is known regarding the enzymes of glycogen metabolism in T. vaginalis. We had previously described the identification and characterization of two forms of glycogen phosphorylase in the organism...
July 2017: Biochimie
https://www.readbyqxmd.com/read/28460054/effect-of-halotolerant-rhizobacteria-isolated-from-halophytes-on-the-growth-of-sugar-beet-beta-vulgaris-l-under-salt-stress
#15
Na Zhou, Shuai Zhao, Chang-Yan Tian
Utilization of rhizobacteria that have associated with plant roots in harsh environments could be a feasible strategy to deal with limits to agricultural production caused by soil salinity. Halophytes occur naturally in high-salt environments, and their roots may be associated with promising microbial candidates for promoting growth and salt tolerance in crops. This study aimed to isolate efficient halotolerant plant-growth-promoting rhizobacterial strains from halophytes and evaluate their activity and effects on sugar beet (Beta vulgaris L...
June 15, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/28446907/regulation-and-molecular-basis-of-environmental-muropeptide-uptake-and-utilization-in-fastidious-oral-anaerobe-tannerella-forsythia
#16
Angela Ruscitto, Kiyonobu Honma, Vamsee M Veeramachineni, Kiyoshi Nishikawa, Graham P Stafford, Ashu Sharma
Tannerella forsythia is a Gram-negative oral anaerobe associated with periodontitis. This bacterium is auxotrophic for the peptidoglycan amino sugar N-acetylmuramic (MurNAc) and likely relies on scavenging peptidoglycan fragments (muropeptides) released by cohabiting bacteria during their cell wall recycling. Many Gram-negative bacteria utilize an inner membrane permease, AmpG, to transport peptidoglycan fragments into their cytoplasm. In the T. forsythia genome, the Tanf_08365 ORF has been identified as a homolog of AmpG permease...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28370477/inducer-exclusion-in-firmicutes-insights-into-the-regulation-of-a-carbohydrate-atp-binding-cassette-transporter-from-lactobacillus-casei-bl23-by-the-signal-transducing-protein-p-ser46-hpr
#17
Constanze Homburg, Martin Bommer, Steven Wuttge, Carolin Hobe, Sebastian Beck, Holger Dobbek, Josef Deutscher, Anke Licht, Erwin Schneider
Catabolite repression is a mechanism that enables bacteria to control carbon utilization. As part of this global regulatory network, components of the phosphoenolpyruvate:carbohydrate phosphotransferase system inhibit the uptake of less favorable sugars when a preferred carbon source such as glucose is available. This process is termed inducer exclusion. In bacteria belonging to the phylum Firmicutes, HPr, phosphorylated at serine 46 (P-Ser46-HPr) is the key player but its mode of action is elusive. To address this question at the level of purified protein components, we have chosen a homolog of the Escherichia coli maltose/maltodextrin ATP-binding cassette transporter from Lactobacillus casei (MalE1-MalF1G1K12 ) as a model system...
March 30, 2017: Molecular Microbiology
https://www.readbyqxmd.com/read/28344648/cellulosic-ethanol-production-via-consolidated-bioprocessing-by-a-novel-thermophilic-anaerobic-bacterium-isolated-from-a-himalayan-hot-spring
#18
Nisha Singh, Anshu S Mathur, Deepak K Tuli, Ravi P Gupta, Colin J Barrow, Munish Puri
BACKGROUND: Cellulose-degrading thermophilic anaerobic bacterium as a suitable host for consolidated bioprocessing (CBP) has been proposed as an economically suited platform for the production of second-generation biofuels. To recognize the overall objective of CBP, fermentation using co-culture of different cellulolytic and sugar-fermenting thermophilic anaerobic bacteria has been widely studied as an approach to achieving improved ethanol production. We assessed monoculture and co-culture fermentation of novel thermophilic anaerobic bacterium for ethanol production from real substrates under controlled conditions...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28335448/a-community-multi-omics-approach-towards-the-assessment-of-surface-water-quality-in-an-urban-river-system
#19
David J Beale, Avinash V Karpe, Warish Ahmed, Stephen Cook, Paul D Morrison, Christopher Staley, Michael J Sadowsky, Enzo A Palombo
A multi-omics approach was applied to an urban river system (the Brisbane River (BR), Queensland, Australia) in order to investigate surface water quality and characterize the bacterial population with respect to water contaminants. To do this, bacterial metagenomic amplicon-sequencing using Illumina next-generation sequencing (NGS) of the V5-V6 hypervariable regions of the 16S rRNA gene and untargeted community metabolomics using gas chromatography coupled with mass spectrometry (GC-MS) were utilized. The multi-omics data, in combination with fecal indicator bacteria (FIB) counts, trace metal concentrations (by inductively coupled plasma mass spectrometry (ICP-MS)) and in-situ water quality measurements collected from various locations along the BR were then used to assess the health of the river ecosystem...
March 14, 2017: International Journal of Environmental Research and Public Health
https://www.readbyqxmd.com/read/28299315/rna-based-stable-isotope-probing-suggests-allobaculum-spp-as-particularly-active-glucose-assimilators-in-a-complex-murine-microbiota-cultured-in-vitro
#20
Elena Herrmann, Wayne Young, Douglas Rosendale, Verena Reichert-Grimm, Christian U Riedel, Ralf Conrad, Markus Egert
RNA-based stable isotope probing (RNA-SIP) and metabolic profiling were used to detect actively glucose-consuming bacteria in a complex microbial community obtained from a murine model system. A faeces-derived microbiota was incubated under anaerobic conditions for 0, 2, and 4 h with 40 mM [U(13)C]glucose. Isopycnic density gradient ultracentrifugation and fractionation of isolated RNA into labeled and unlabeled fractions followed by 16S rRNA sequencing showed a quick adaptation of the bacterial community in response to the added sugar, which was dominated by unclassified Lachnospiraceae species...
2017: BioMed Research International
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