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https://www.readbyqxmd.com/read/28088760/usp15-regulates-dynamic-protein-protein-interactions-of-the-spliceosome-through-deubiquitination-of-prp31
#1
Tanuza Das, Joon Kyu Park, Jinyoung Park, Eunji Kim, Michael Rape, Eunice EunKyeong Kim, Eun Joo Song
Post-translational modifications contribute to the spliceosome dynamics by facilitating the physical rearrangements of the spliceosome. Here, we report USP15, a deubiquitinating enzyme, as a regulator of protein-protein interactions for the spliceosome dynamics. We show that PRP31, a component of U4 snRNP, is modified with K63-linked ubiquitin chains by the PRP19 complex and deubiquitinated by USP15 and its substrate targeting factor SART3. USP15(SART3) makes a complex with USP4 and this ternary complex serves as a platform to deubiquitinate PRP31 and PRP3...
January 13, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/28081489/protective-effect-of-chitosan-on-photosynthesis-and-antioxidative-defense-system-in-edible-rape-brassica-rapa-l-in-the-presence-of-cadmium
#2
Haiying Zong, Song Liu, Ronge Xing, Xiaolin Chen, Pengcheng Li
Chitosan (CTS) induces plant tolerance against several abiotic stresses, including salinity and drought exposure. However, the role of CTS in cadmium (Cd)-induced stress amelioration is largely unknown. In the present study, a hydroponic pot experiment was conducted to study the roles of CTS with different molecular weight (Mw) (10kDa,5kDa and 1kDa) in alleviating Cd toxicity in edible rape (Brassica rapa L .). The results showed that Cd stress significantly decreased plant growth, leaf chlorophyll contents and increased the malondialdehyde (MDA) level in rape leaves...
January 9, 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/28078831/perception-on-the-abortion-laws-in-sri-lanka-a-community-based-study-in-the-city-of-colombo
#3
M S Suranga, K T Silva, L Senanayake
Introduction: Abortion is legally permitted in Sri Lanka, only if it is performed to save the mother’s life. However, it is estimated that a large number of induced abortions take place in Sri Lanka. Knowledge and attitudes towards induced abortion in the society are key issues influencing the policy response towards changes in the law. This study aimed to assess the knowledge and attitudes of adults towards induced abortion in Sri Lanka. Methods: Six Grama Niladhari Divisions (GNDs) and five to eight housing clusters from each GND were selected from Thimbirigasyaya Divisional Secretariat Division using multi stage stratified random sampling...
30, 2016: Ceylon Medical Journal
https://www.readbyqxmd.com/read/28076392/arthropod-pest-control-for-uk-oilseed-rape-comparing-insecticide-efficacies-side-effects-and-alternatives
#4
Han Zhang, Tom Breeze, Alison Bailey, David Garthwaite, Richard Harrington, Simon G Potts
Oilseed rape (Brassica napus) is an important combinable break crop in the UK, which is largely protected from arthropod pests by insecticidal chemicals. Despite ongoing debate regarding the use of neonicotinoids, the dominant seed treatment ingredients used for this crop, there is little publicly available data comparing the efficacy of insecticides in controlling key arthropod pests or comparing the impacts on non-target species and the wider environment. To provide an insight into these matters, a UK-wide expert survey targeting agronomists and entomologists was conducted from March to June 2015...
2017: PloS One
https://www.readbyqxmd.com/read/28071792/thermal-stability-improvement-of-rice-bran-albumin-protein-incorporated-with-epigallocatechin-gallate
#5
Zhongkai Zhou, Jingjing Xu, Yuqian Liu, Demei Meng, Xiaoli Sun, Hong Yi, Yunjing Gao, Guoyu Sun, Padraig Strappe, Chris Blanchard, Rui Yang
Rice bran albumin protein (RAP) is sensitive to thermal changes and tends to degrade when exposed to high-temperature processing. In this work, RAP-epigallocatechin-3-gallate (EGCG) complex (RAPE) was prepared and the thermal stability was evaluated. Fluorescence results showed that EGCG could interact with RAP with a binding number n of 0.0885:1 (EGCG:RAP, w/w) and a binding constant K of 1.02 (± 0.002) ×10(4) /M, suggesting both hydrogen bonding and van der Waals forces played an important role. FTIR analysis demonstrated that EGCG could induce secondary structural changes in RAP above a ratio of 1...
January 10, 2017: Journal of Food Science
https://www.readbyqxmd.com/read/28071031/study-of-variation-of-tocochromanol-and-phytosterol-contents-in-black-and-yellow-seeds-of-brassica-napus-l-doubled-haploid-populations
#6
Teresa Cegielska-Taras, Małgorzata Nogala-Kałucka, Laurencja Szala, Aleksander Siger
BACKGROUND: In the study, an analysis of tocopherols, plastochomanol-8 and phytosterols was conducted using DH lines obtained from F1 hybrids of reciprocal crosses between yellow- and black-seeded lines. METHODS: The biological material for the study consisted of two DH populations of winter oilseed rape obtained from F1 hybrids of reciprocal crosses between two DH lines: yellow- and black-seeded. Seed color was determined using a ColorFlex spectrophotometer. Fat content was determined via pulsed NMR...
July 2016: Acta Scientiarum Polonorum. Technologia Alimentaria
https://www.readbyqxmd.com/read/28071028/influence-of-impurities-in-raw-material-on-sensory-and-physicochemical-properties-of-cold-pressed-rapeseedoil-produced-from-conventionally-and-ecologically-grown-seeds
#7
Małgorzata Wroniak, Agnieszka Rękas, Katarzyna Ratusz
BACKGROUND: The main problem of cold-pressed technology is its low yield and the varying quality of the end product, which is dependent on the quality of the raw material, technological process applied and also on conditions of packaging and storage. The effects of different contents of impurities in rapeseed on the sensory, physicochemical properties and oxidative stability of rapeseed oil produced by cold-pressing were investigated. METHODS: Cold-pressed oil produced from conventionally grown rapeseeds (individual cultivars and industrial seeds) and certified ecological rapeseeds...
July 2016: Acta Scientiarum Polonorum. Technologia Alimentaria
https://www.readbyqxmd.com/read/28068426/screening-of-candidate-leaf-morphology-genes-by-integration-of-qtl-mapping-and-rna-sequencing-technologies-in-oilseed-rape-brassica-napus-l
#8
Hongju Jian, Bo Yang, Aoxiang Zhang, Li Zhang, Xinfu Xu, Jiana Li, Liezhao Liu
Leaf size and shape play important roles in agronomic traits, such as yield, quality and stress responses. Wide variations in leaf morphological traits exist in cultivated varieties of many plant species. By now, the genetics of leaf shape and size have not been characterized in Brassica napus. In this study, a population of 172 recombinant inbred lines (RILs) was used for quantitative trait locus (QTL) analysis of leaf morphology traits. Furthermore, fresh young leaves of extreme lines with more leaf lobes (referred to as 'A') and extreme lines with fewer lobes (referred to as 'B') selected from the RIL population and leaves of dissected lines (referred to as 'P') were used for transcriptional analysis...
2017: PloS One
https://www.readbyqxmd.com/read/28065969/survellience-system-for-child-abuse-bridging-the-gap-between-actual-and-hidden-cases
#9
Maryam Pyar Ali Lakhdir, Yasmin Parpio, Salima Farooq
The dynamics of child abuse have been recognised as a global emerging social issue which is highly associated with child humiliation. The Government of Pakistan has taken many initiatives for addressing issues regarding child abuse and neglect and has designed laws to protect children against exploitation, abuse and violence; however, most of them are not enforced at all or poorly implemented due to lack of proper definition of child abuse and related terms, and different ways of reporting child abuse cases...
January 2017: JPMA. the Journal of the Pakistan Medical Association
https://www.readbyqxmd.com/read/28060974/-socio-demographic-profile-and-factors-associated-with-emergency-contraception-consultations
#10
Sebastián Lavanderos, Camilo Riquelme, Juan Haase, Andrea Morales
BACKGROUND: Emergency contraception refers to contraceptive methods that can be used after a sexual intercourse Aim: To analyze the evolution of emergency contraception (EC) consultations on the Chilean public health system, at a communal level, in relation to the legal changes that recently took place, aimed to ease the delivery of the benefit. To analyze its association with socioeconomic, demographic and municipal healthcare system characteristics. MATERIAL AND METHODS: We analyzed data bases of the Ministry of Health to study EC consultation rates on Chilean communes, including consultations on emergency departments and by rape, from 2008 to 2013...
September 2016: Revista Médica de Chile
https://www.readbyqxmd.com/read/28060583/the-use-of-vignettes-to-empower-effective-responses-to-attempted-sexual-assault
#11
Kaylie T Allen, Elizabeth A Meadows
OBJECTIVE: Women assertively resisting sexual aggression have the best chances of avoiding completed rape. Especially with acquaintances, there are significant social and psychological barriers to resistance. Novel vignettes depicting acquaintance rape were designed to enhance self-efficacy, reduce unrealistic optimism, and empower assertive resistance. PARTICIPANTS: The data were collected using a Web-based survey of 449 female college students from multiple universities in August-October 2014...
January 6, 2017: Journal of American College Health: J of ACH
https://www.readbyqxmd.com/read/28055082/association-of-dsm-iv-posttraumatic-stress-disorder-with-traumatic-experience-type-and-history-in-the-world-health-organization-world-mental-health-surveys
#12
Howard Liu, Maria V Petukhova, Nancy A Sampson, Sergio Aguilar-Gaxiola, Jordi Alonso, Laura Helena Andrade, Evelyn J Bromet, Giovanni de Girolamo, Josep Maria Haro, Hristo Hinkov, Norito Kawakami, Karestan C Koenen, Viviane Kovess-Masfety, Sing Lee, Maria Elena Medina-Mora, Fernando Navarro-Mateu, Siobhan O'Neill, Marina Piazza, José Posada-Villa, Kate M Scott, Victoria Shahly, Dan J Stein, Margreet Ten Have, Yolanda Torres, Oye Gureje, Alan M Zaslavsky, Ronald C Kessler
Importance: Previous research has documented significant variation in the prevalence of posttraumatic stress disorder (PTSD) depending on the type of traumatic experience (TE) and history of TE exposure, but the relatively small sample sizes in these studies resulted in a number of unresolved basic questions. Objective: To examine disaggregated associations of type of TE history with PTSD in a large cross-national community epidemiologic data set. Design, Setting, and Participants: The World Health Organization World Mental Health surveys assessed 29 TE types (lifetime exposure, age at first exposure) with DSM-IV PTSD that was associated with 1 randomly selected TE exposure (the random TE) for each respondent...
January 4, 2017: JAMA Psychiatry
https://www.readbyqxmd.com/read/28050618/clubroot-resistance-qtl-are-modulated-by-nitrogen-input-in-brassica-napus
#13
A Laperche, Y Aigu, M Jubault, M Ollier, S Guichard, P Glory, S E Strelkov, A Gravot, M J Manzanares-Dauleux
Nitrogen levels can modulate the effectiveness of clubroot resistance in an isolate- and host-specific manner. While the same QTL were detected under high and low nitrogen, their effects were altered. Clubroot, caused by Plasmodiophora brassicae, is one of the most damaging diseases of oilseed rape and is known to be affected by nitrogen fertilization. However, the genetic factors involved in clubroot resistance have not been characterized under nitrogen-limiting conditions. This study aimed to assess the variability of clubroot resistance under different nitrogen levels and to characterize the impact of nitrogen supply on genetic resistance factors...
January 3, 2017: TAG. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik
https://www.readbyqxmd.com/read/28045929/detecting-the-hormonal-pathways-in-oilseed-rape-behind-induced-systemic-resistance-by-trichoderma-harzianum-th12-to-sclerotinia-sclerotiorum
#14
Jawadayn Talib Alkooranee, Tamarah Raad Aledan, Ali Kadhim Ali, Guangyuan Lu, Xuekun Zhang, Jiangsheng Wu, Chunhua Fu, Maoteng Li
Plants have the ability to resist pathogen attack after infection or treatment with biotic and abiotic elicitors. In oilseed rape plant Brassica napus AACC and in the artificially synthesized Raphanus alboglabra RRCC, the root-colonizing Trichoderma harzianum TH12 fungus triggers induced systemic resistance (ISR), and its culture filtrate (CF) triggers a systemic acquired resistance (SAR) response against infection by the Sclerotinia sclerotiorum. Salicylic acid (SA) and jasmonic acid/ethylene (JA/ET) are plant hormone signals that play important roles in the regulation of ISR and SAR...
2017: PloS One
https://www.readbyqxmd.com/read/28043336/sensitivity-and-biochemical-characteristics-of-sclerotinia-sclerotiorum-to-propamidine
#15
Yong Wang, Yang Sun, Ying Zhang, Yinxing Zhang, Lirong Han, Xing Zhang, Juntao Feng
Propamidine is an aromatic diamidine compound. In the current study, baseline sensitivity of Sclerotinia sclerotiorum to propamidine was determined using 78 strains collected from the oilseed rape fields without a previous history of propamidine usage. The median effective concentration (EC50) values for propamidine inhibiting mycelial growth ranged from 0.406 to 3.647μg/mL, with a mean of 1.616±0.217μg/mL. There was no correlation between sensitivity to propamidine and sensitivity to dimethachlon or carbendazim...
January 2017: Pesticide Biochemistry and Physiology
https://www.readbyqxmd.com/read/28032694/simultaneous-determination-of-thiamethoxam-clothianidin-and-metazachlor-residues-in-soil-by-ultra-high-performance-liquid-chromatography-coupled-to-quadrupole-time-of-flight-mass-spectrometry
#16
Silvia Valverde, Ana M Ares, José Luis Bernal, María Jesús Nozal, José Bernal
A rapid pioneering method has been developed to simultaneously determine residues of three pesticides (thiamethoxam, clothianidin and metazachlor) in soil by ultra high performance liquid chromatography coupled to a mass spectrometry detector (quadrupole time-of-flight). An efficient extraction procedure (90-105% average analyte recoveries) has also been proposed, involving solid-liquid extraction by a mixture of water and methanol (60:40, v/v), centrifugation and concentration. A chromatographic analysis of the compounds was achieved in 5...
December 29, 2016: Journal of Separation Science
https://www.readbyqxmd.com/read/28030996/not-a-big-deal-examining-help-seeking-behaviors-of-sexually-victimized-women-on-the-college-campus
#17
McKenzie Wood, Amy Stichman
The sexual assault and coercion of women on university campuses continues to be an ongoing problem. It is estimated that more than 15% of women attending college experience some type of sexual assault or coercion each year. As universities and other social institutions begin to acknowledge the prevalence of sexual victimization, an increased emphasis has been placed on helping victims of sexual crimes. The current study uses results from 378 surveys completed by females at a midwestern university to examine the help-seeking behaviors of those who have been victimized...
December 1, 2016: International Journal of Offender Therapy and Comparative Criminology
https://www.readbyqxmd.com/read/28029496/accuracy-of-the-estimation-of-dental-age-in-comparison-with-chronological-age-in-a-spanish-sample-of-2641-living-subjects-using-the-demirjian-and-nolla-methods
#18
María Melo, Javier Ata-Ali
Age estimation is an important procedure in forensic medicine and is carried out for a number of reasons. For living persons, age estimation is performed in order to assess whether a child has attained the age of criminal responsibility, in scenarios involving rape, kidnapping or marriage, in premature births, adoption procedures, illegal immigration, pediatric endocrine diseases and orthodontic malocclusion, as well as in circumstances in which the birth certificate is not available or the records are suspect...
October 12, 2016: Forensic Science International
https://www.readbyqxmd.com/read/28024671/reform-of-abortion-law-in-uruguay-context-process-and-lessons-learned
#19
Susan Wood, Lilián Abracinskas, Sonia Correa, Mario Pecheny
In October 2012, a new law was approved in Uruguay that allows abortion on demand during the first 12 weeks of pregnancy, 14 weeks in the case of rape, and without a time limit when the woman's health is at risk or in the case of foetal anomalies. This paper analyses this legal reform. It is based on 27 individual and group interviews with key informants, and on review of primary documents and the literature. The factors explaining the reform include: secular values in society, favourable public opinion, a persistent feminist movement, effective coalition building, particular party politics, and a vocal public health sector...
November 2016: Reproductive Health Matters
https://www.readbyqxmd.com/read/28024267/sexual-homicide-brief-review-of-the-literature-and-case-report-involving-rape-genital-mutilation-and-human-arson
#20
REVIEW
Maria-Valeria Karakasi, Epameinondas Vasilikos, Polichronis Voultsos, Aikaterini Vlachaki, Pavlos Pavlidis
The objective of the current paper is to report a new case of sexual murder involving human arson and summarize the literature on the phenomenon of sexual homicide. The present case study is unprecedented in Greece and a rarity in international literature due to the fact that the victim suffered genital mutilation and incineration while still alive. The evaluation consisted of 176 articles; 53 were reviewed by the authors. The results revealed sparse, but significant, research findings. The authors discuss the limitations regarding research, incidence of the phenomenon, crime-scene patterns, offender characteristics (killing methods, motive inferences, sociodemographic data, classifications, psychopathology, modus operandi), and victim selection...
December 20, 2016: Journal of Forensic and Legal Medicine
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