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https://www.readbyqxmd.com/read/28639170/bistability-and-asynchrony-in-a-boolean-model-of-the-l-arabinose-operon-in-escherichia-coli
#1
Andy Jenkins, Matthew Macauley
The lactose operon in Escherichia coli was the first known gene regulatory network, and it is frequently used as a prototype for new modeling paradigms. Historically, many of these modeling frameworks use differential equations. More recently, Stigler and Veliz-Cuba proposed a Boolean model that captures the bistability of the system and all of the biological steady states. In this paper, we model the well-known arabinose operon in E. coli with a Boolean network. This has several complex features not found in the lac operon, such as a protein that is both an activator and repressor, a DNA looping mechanism for gene repression, and the lack of inducer exclusion by glucose...
June 21, 2017: Bulletin of Mathematical Biology
https://www.readbyqxmd.com/read/28627408/developmental-exposure-to-glyphosate-based-herbicide-and-depressive-like-behavior-in-adult-offspring-implication-of-glutamate-excitotoxicity-and-oxidative-stress
#2
Daiane Cattani, Patrícia Acordi Cesconetto, Mauren Kruger Tavares, Eduardo Benedetti Parisotto, Paulo Alexandre De Oliveira, Carla Elise Heinz Rieg, Marina Concli Leite, Rui Daniel Schröder Prediger, Nestor Cubas Wendt, Guilherme Razzera, Danilo Wilhelm Filho, Ariane Zamoner
We have previously demonstrated that maternal exposure to glyphosate-based herbicide (GBH) leads to glutamate excitotoxicity in 15-day-old rat hippocampus. The present study was conducted in order to investigate the effects of subchronic exposure to GBH on some neurochemical and behavioral parameters in immature and adult offspring. Rats were exposed to 1% GBH in drinking water (corresponding to 0.36% of glyphosate) from gestational day 5 until postnatal day (PND)-15 or PND60. Results showed that GBH exposure during both prenatal and postnatal periods causes oxidative stress, affects cholinergic and glutamatergic neurotransmission in offspring hippocampus from immature and adult rats...
June 13, 2017: Toxicology
https://www.readbyqxmd.com/read/28615850/prevalence-of-giardiaduodenalis-among-children-from-a-central-region-of-cuba-molecular-characterization-and-associated-risk-factors
#3
Luis Jerez Puebla, Fidel A Núñez, Alexey Brito García, Lázara Rojas Rivero, Irais Atencio Millán, Raúl Cordoví Prado
Giardia duodenalis is one of the most frequent intestinal parasitic infections in children worldwide. To date, eight main assemblages of G. duodenalis have been described, but only A and B genetic groups are known to infect humans. A cross-sectional study was conducted to determine the prevalence and risk factors of Giardia duodenalis infection in 417 preschool children from the Fomento municipality in the central region of Cuba between January and June 2013. The overall prevalence of Giardia infection was 10...
June 2017: Journal of Parasitic Diseases: Official Organ of the Indian Society for Parasitology
https://www.readbyqxmd.com/read/28614487/actual-and-predicted-prevalence-of-alcohol-consumption-during-pregnancy-in-latin-america-and-the-caribbean-systematic-literature-review-and-meta-analysis
#4
Shannon Lange, Charlotte Probst, Navrose Heer, Michael Roerecke, Jürgen Rehm, Maristela G Monteiro, Kevin Shield, Claire de Oliveira, Svetlana Popova
Objective: To estimate the prevalence of alcohol consumption during pregnancy among the general population of Latin America and the Caribbean, by country, in 2012. Methods: Three steps were taken: a comprehensive, systematic literature search; meta-analyses, assuming a random-effects model for countries with published studies; and regression modelling (data prediction) for countries with either no published studies or too few to obtain an estimate. Results: Based on 24 existing studies, the pooled prevalence of alcohol consumption during pregnancy among the general population was estimated for Brazil (15...
June 8, 2017: Revista Panamericana de Salud Pública, Pan American Journal of Public Health
https://www.readbyqxmd.com/read/28612173/evaluation-of-the-feeding-value-of-dichrostachys-cinerea-pods-for-fattening-pigs-in-cuba
#5
N Martín-Casas, M Reinoso-Pérez, J R García-Díaz, H H Hansen, M O Nielsen
Dichrostachys cinerea (L.) Wight & Arn. is a tropical leguminous shrub widely regarded as an invasive species in Cuba, after having invaded a significant proportion of its arable land during the past decades. Concurrently, smallholder pig producers are highly constrained by the scarcity of protein feeds. This study aimed to assess the feeding value of D. cinerea pod meal (DCPM) as an alternative protein supplement for pigs in Cuban smallholder production systems. An on-farm feeding trial was carried out with three groups (N = 10) of growing-fattening pigs over 60 days, where DCPM replaced 0, 15, and 30% in DM of a dietary commercial concentrate...
June 13, 2017: Tropical Animal Health and Production
https://www.readbyqxmd.com/read/28610000/the-hydraenidae-of-cuba-insecta-coleoptera-iii-description-of-two-new-hygropetric-species-of-i-hydraena-i-kugelann
#6
Albert Deler-Hernández, Martin Fikáček, Juan A Delgado
Two new species of the genus Hydraena Kugelann, 1794 collected from hygropetric habitats in eastern Cuba are described: Hydraena (Hydraenopsis) blancae sp. nov. from the Nipe-Sagua-Baracoa mountain range, and Hydraena (Hydraenopsis) matthiasi sp. nov. from the Sierra Maestra mountain range. Both species, especially the latter, are closely related to Hydraena (Hydraenopsis) franklyni Deler-Hernández & Delgado, 2012. Diagnostic characters for both new species are provided and illustrated; habitat information and distributional data are also included...
April 7, 2017: Zootaxa
https://www.readbyqxmd.com/read/28609929/a-new-anchialine-species-of-i-naushonia-i-decapoda-gebiidea-laomediidae-from-the-bahamas
#7
Fernando Alvarez, Thomas M Iliffe, José Luis Villalobos
A new species of mud shrimp of the genus Naushonia Kingsley, 1897 is described from two anchialine caves on the island of Great Abaco in the Bahamas. Naushonia tinkeri n. sp. is the fifteenth species in the genus and the second to be described from the Bahamas. The new species is morphologically similar to N. augudrea (Juarrero & García, 1997) from Holguín Province, eastern Cuba, with which it shares a carapace with cervical and cardiac grooves; however, it can be distinguished by having a pigmented cornea, the first pereiopod with a proportionately longer propodus and dactylus without a toothed external margin, and the telson longer relative to the uropod length...
April 27, 2017: Zootaxa
https://www.readbyqxmd.com/read/28608285/the-glp-1-analogue-lixisenatide-decreases-atherosclerosis-in-insulin-resistant-mice-by-modulating-macrophage-phenotype
#8
Ángela Vinué, Jorge Navarro, Andrea Herrero-Cervera, Marta García-Cubas, Irene Andrés-Blasco, Sergio Martínez-Hervás, José T Real, Juan F Ascaso, Herminia González-Navarro
AIMS/HYPOTHESIS: Recent clinical studies indicate that glucagon-like peptide-1 (GLP-1) analogues prevent acute cardiovascular events in type 2 diabetes mellitus but their mechanisms remain unknown. In the present study, the impact of GLP-1 analogues and their potential underlying molecular mechanisms in insulin resistance and atherosclerosis are investigated. METHODS: Atherosclerosis development was evaluated in Apoe (-/-) Irs2 (+/-) mice, a mouse model of insulin resistance, the metabolic syndrome and atherosclerosis, treated with the GLP-1 analogues lixisenatide or liraglutide...
June 12, 2017: Diabetologia
https://www.readbyqxmd.com/read/28590216/diagnostic-doses-of-insecticides-for-adult-aedes-aegypti-to-assess-insecticide-resistance-in-cuba
#9
María Magdalena Rodríguez, Ariel Crespo, Daymi Hurtado, Ilario Fuentes, Jorge Rey, Juan Andrés Bisset
The objective of this study was to determine diagnostic doses (DDs) of 5 insecticides for the Rockefeller susceptible strain of Aedes aegypti , using the Centers for Disease Control and Prevention (CDC) bottle bioassay as a tool for monitoring insecticide resistance in the Cuban vector control program. The 30-min DD values determined in this study were 13.5 μg/ml, 6.5 μg/ml, 6 μg/ml, 90.0 μg/ml, and 15.0 μg/ml for cypermethrin, deltamethrin, lambda-cyhalothrin, chlorpyrifos, and propoxur, respectively...
June 2017: Journal of the American Mosquito Control Association
https://www.readbyqxmd.com/read/28588590/a-conserved-carbon-starvation-response-underlies-bud-dormancy-in-woody-and-herbaceous-species
#10
Carlos Tarancón, Eduardo González-Grandío, Juan C Oliveros, Michael Nicolas, Pilar Cubas
Plant shoot systems give rise to characteristic above-ground plant architectures. Shoots are formed from axillary meristems and buds, whose growth and development is modulated by systemic and local signals. These cues convey information about nutrient and water availability, light quality, sink/source organ activity and other variables that determine the timeliness and competence to maintain development of new shoots. This information is translated into a local response, in meristems and buds, of growth or quiescence...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28588565/diversity-of-ligninolytic-enzymes-and-their-genes-in-strains-of-the-genus-ganoderma-applicable-for-biodegradation-of-xenobiotic-compounds
#11
Giselle Torres-Farradá, Ana M Manzano León, François Rineau, Lucía L Ledo Alonso, María I Sánchez-López, Sofie Thijs, Jan Colpaert, Miguel Ramos-Leal, Gilda Guerra, Jaco Vangronsveld
White-rot fungi (WRF) and their ligninolytic enzymes (laccases and peroxidases) are considered promising biotechnological tools to remove lignin related Persistent Organic Pollutants from industrial wastewaters and contaminated ecosystems. A high diversity of the genus Ganoderma has been reported in Cuba; in spite of this, the diversity of ligninolytic enzymes and their genes remained unexplored. In this study, 13 native WRF strains were isolated from decayed wood in urban ecosystems in Havana (Cuba). All strains were identified as Ganoderma sp...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28582382/predictive-model-for-the-development-of-hypertensive-cardiopathy-a-prospective-cohort-study
#12
Alexis Álvarez Aliaga, Julio César González-Aguilera, Liliana Del Rosario Maceo-Gómez, Alexis Suárez-Quesada
INTRODUCTION: Predictive models of cardiovascular conditions are useful tools for risk stratification. The high morbidity and mortality resulting from hypertensive cardiopathy creates a need for the use of tools to predict the risk of cardiovascular disease. OBJECTIVE: To evaluate the capacity of a model based on risk factors to predict the development of hypertensive cardiopathy after ten years in patients with a diagnosis of essential arterial hypertension. METHODS: A prospective cohort study was carried out in hypertensive patients cared for at the specialized arterial hypertension physician’s office of the Specialty Policlinic attached to “Carlos Manuel de Céspedes” Hospital, Bayamo Municipality, Granma Province, Cuba, from January 1, 2000 to December 31, 2009...
May 9, 2017: Medwave
https://www.readbyqxmd.com/read/28562791/terms-of-the-specialized-nursing-language-for-the-care-of-ostomates
#13
Carina Maris Gaspar Carvalho, Marcia Regina Cubas, Maria Miriam Lima da Nóbrega
Objectives: to identify terms of the specialized nursing language for the care of ostomates from the literature of the area, and to map the identified terms with terms of the International Classification for Nursing Practice (ICNP®). Method: descriptive study of quantitative approach guided by the guidelines for the elaboration of terminology subsets of the ICNP®. The terms were collected in 49 scientific articles, extracted using a computational tool, selected according to the relevance for the theme, and normalized and mapped with the ICNP®...
May 2017: Revista Brasileira de Enfermagem
https://www.readbyqxmd.com/read/28562764/serosurvey-of-anti-leptospira-sp-and-anti-toxoplasma-gondii-antibodies-in-capybaras-and-collared-and-white-lipped-peccaries
#14
Leila Sabrina Ullmann, Mara Lúcia Gravinatti, Ricardo Seiti Yamatogi, Leonilda Correa Dos Santos, Wanderlei de Moraes, Zalmir Silvino Cubas, Lucilene Granuzzio Camossi, Ivan Roque de Barros, Helio Langoni, Rafael Felipe da Costa Vieira, Alexander Welker Biondo
INTRODUCTION: Brazilian native species are reemerging as increasingly free-ranging populations. METHODS: Sera from 31 capybaras (Hydrochoerus hydrochaeris) and 28 peccaries (Pecari tajacu and Tayassu pecari) were tested for anti-Leptospira and anti-Toxoplasma gondii antibodies using microscopic seroagglutination test. RESULTS: Nineteen percent of free-ranging and 10.0% of captive capybaras, along with 31.8% of collared peccaries, were seropositive for T...
March 2017: Revista da Sociedade Brasileira de Medicina Tropical
https://www.readbyqxmd.com/read/28559798/in-situ-rt-pcr-optimized-for-electron-microscopy-allows-description-of-subcellular-morphology-of-target-mrna-expressing-cells-in-the-brain
#15
Laura Cubas-Nuñez, María Duran-Moreno, Jessica Castillo-Villalba, Jorge Fuentes-Maestre, Bonaventura Casanova, José M García-Verdugo, Sara Gil-Perotín
In situ RT-PCR detects and amplifies mRNA (cDNA) while obtaining spatial information of gene expression. When the intended use is an ultrastructural analysis of morphology, the procedure may be technically challenging and quality of tissue dramatically altered by proteolytic digestion and extreme astringency and temperature conditions. We describe a low-damaging protocol of in situ RT-PCR combined to conventional electron microscopy that preserves fine morphology, increases sensitivity, and decreases costs and complexity associated to RNA probes...
2017: Frontiers in Cellular Neuroscience
https://www.readbyqxmd.com/read/28546990/genetics-and-genomic-medicine-in-cuba
#16
Hilda Roblejo Balbuena, Beatriz Marcheco Teruel
Genetics and genomic medicine in Cuba.
May 2017: Molecular Genetics & Genomic Medicine
https://www.readbyqxmd.com/read/28540254/doppler-ultrasonography-of-the-pectinis-oculi-artery-in-harpy-eagles-harpia-harpyja
#17
Wanderlei de Moraes, Thiago A C Ferreira, André T Somma, Zalmir S Cubas, Bret A Moore, Fabiano Montiani-Ferreira
Twenty harpy eagles (Harpia harpyja) without systemic or ocular diseases were examined to measure blood velocity parameters of the pectinis oculi artery using Doppler ultrasonography. Pectinate artery resistive index (RI) and pulsatility index (PI) were investigated using ocular Doppler ultrasonography. The mean RI and PI values across all eyes were 0.44±0.10 and 0.62±0.20 respectively. Low RI and PI values found in the harpy eagle´s pectinis oculi artery compared with the American pekin ducks one and other tissue suggest indeed a high metabolic activity in pecten oculi and corroborates the hypothesis of a nutritional function and/or intraocular pressure regulation...
2017: Open veterinary journal
https://www.readbyqxmd.com/read/28539192/shared-decision-making-and-the-promise-of-a-respectful-and-equitable-healthcare-system-in-peru
#18
Germán Málaga, Zoila O Romero, Ariana S Málaga, Sofia Cuba-Fuentes
Peru has achieved sustained development in the last two decades. However, despite this achievement, it has not been matched with improvements in the quality of education and health; investment in both sectors is among the lowest in the region. This situation perpetuates huge gaps in infrastructure and also conditions a poor literacy level of the population specifically in health. Currently, there is a fragmented model of patient care, in which the systems are exclusive of each other. They do not cooperate or communicate with each other; and if there is no vertical communication within the system, preventing referral of patients directly from the basic level to the complex level of care when needed...
May 20, 2017: Zeitschrift Für Evidenz, Fortbildung und Qualität Im Gesundheitswesen
https://www.readbyqxmd.com/read/28538842/-diagnostic-concordance-between-seven-definitions-of-metabolic-syndrome-in-overweight-and-obese-adults
#19
Eduardo Cabrera-Rode, Beatriz Stusser, Wenny Cálix, Neraldo Orlandi, Janet Rodríguez, Ileana Cubas-Dueñas, Ragmila Echevarría, Aimee Álvarez
Objectives: The aim of this study was to determine the level of diagnostic concordance between seven definitions of metabolic syndrome (MS) in a group of overweight and obese adults. Materials and Methods: 350 subjects aged from 19 to 70 years were recruited for study from a clinic for overweight and obese subjects. The definitions of MS used were those given by the WHO (World Health Organization), EGIR (European Group for the Study of Insulin Resistance), NCEP- ATPIII (Adult Treatment Panel), AHA/NHLBI (American Heart Association), IDF (International Diabetes Federation), and JIS (Joint Interim Statement) as well as the Szabo criteria...
January 2017: Revista Peruana de Medicina Experimental y Salud Pública
https://www.readbyqxmd.com/read/28525651/selecting-elephant-grass-families-and-progenies-to-produce-bioenergy-through-mixed-models-reml-blup
#20
E V Rodrigues, R F Daher, A Dos Santos, M Vivas, J C Machado, G do A Gravina, Y P de Souza, A K Vidal, A Dos S Rocha, R S Freitas
Brazil has great potential to produce bioenergy since it is located in a tropical region that receives high incidence of solar energy and presents favorable climatic conditions for such purpose. However, the use of bioenergy in the country is below its productivity potential. The aim of the current study was to select full-sib progenies and families of elephant grass (Pennisetum purpureum S.) to optimize phenotypes relevant to bioenergy production through mixed models (REML/BLUP). The circulating diallel-based crossing of ten elephant grass genotypes was performed...
May 18, 2017: Genetics and Molecular Research: GMR
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