keyword
MENU ▼
Read by QxMD icon Read
search

RPTEC

keyword
https://www.readbyqxmd.com/read/27924516/carbon-monoxide-inhibits-the-nuclear-cytoplasmic-translocation-of-hmgb1-in-an-in-vitro-oxidative-stress-injury-model-of-mouse-renal-tubular-epithelial-cells
#1
Yu Jia, Lu Wang, Guang-Yuan Zhao, Zhi-Qiang Wang, Song Chen, Gang Chen
Carbon monoxide (CO), as a vital small molecule in signaling pathways, is found to be involved in ischemia-reperfusion injury (IRI) in renal transplantation. CO-releasing molecule-2 (CORM-2), a CO-releasing molecule, is a type of metal carbonyl complexes which can quickly release CO in vivo. In this study, an in vitro oxidative stress injury model was established to examine the effect of CORM-2 pretreatment on the nuclear-cytoplasmic translocation of high mobility group box 1 protein (HMGB1) in mouse primary renal proximal tubular epithelial cells (RPTECs)...
December 2016: Journal of Huazhong University of Science and Technology. Medical Sciences
https://www.readbyqxmd.com/read/27755992/a-new-form-of-amphotericin-b-the-complex-with-copper-ii-ions-downregulates-stnfr1-shedding-and-changes-the-activity-of-genes-involved-in-tnf-induced-pathways-amb-cu-2-downregulates-stnfr1-shedding-and-changes-the-activity-of-genes-involved-in-tnf-induced-pathways
#2
Joanna Gola, Barbara Strzałka-Mrozik, Celina Kruszniewska-Rajs, Adrian Janiszewski, Bartłomiej Skowronek, Mariusz Gagoś, Grzegorz Czernel, Urszula Mazurek
BACKGROUND: A new form of amphotericin B (AmB)- complex with copper (II) ions (AmB-Cu(2+)) - is less toxic to human renal cells. Cytokines, including Tumor Necrosis Factor (TNF), are responsible for nephrotoxicity observed in patients treated with AmB. Another problem during therapy is the occurrence of oxidized forms of AmB (AmB-ox) in patients' circulation. To elucidate the molecular mechanism responsible for the reduction of the toxicity of AmB-Cu(2+), we evaluated the expression of genes encoding TNF and its receptors alongside encoding proteins involved in TNF-induced signalization...
September 5, 2016: Pharmacological Reports: PR
https://www.readbyqxmd.com/read/27616666/gene-expression-study-of-phase-i-and-ii-metabolizing-enzymes-in-rptec-tert1-cell-line-application-in-in-vitro-nephrotoxicity-prediction
#3
Heta Shah, Manish Patel, Neeta Shrivastava
1. The phase I and II metabolizing enzymes of kidneys play an important role in the metabolism of xenobiotic as well as endogenous compounds and proximal tubules of kidney constitute high concentration of these metabolizing enzymes compared with the other parts. 2. It has been shown previously that differential enzyme expression among human and rodent/non-rodent species can be a roadblock in drug discovery and development process. Currently, proximal tubule cell lines of human origin such as RPTEC/TERT1 and HK-2 are used to understand the pathophysiology of kidney diseases, therapeutic efficacy of drugs, and nephrotoxicity of compounds...
October 3, 2016: Xenobiotica; the Fate of Foreign Compounds in Biological Systems
https://www.readbyqxmd.com/read/27602490/renal-tubular-epithelium-targeted-peroxisome-proliferator-activated-receptor-%C3%AE-maintains-the-epithelial-phenotype-and-antagonizes-renal-fibrogenesis
#4
Min Zhao, Ying Chen, Guixia Ding, Ying Xu, Mi Bai, Yue Zhang, Zhanjun Jia, Songming Huang, Aihua Zhang
Accumulating evidence suggests that loss of the renal tubular epithelial phenotype plays an important role in the pathogenesis of renal tubulointerstitial fibrosis. Systemic activation of peroxisome proliferator-activated receptor γ (PPAR-γ) has been shown to be protective against renal fibrosis, although the mechanisms are poorly understood. The present study aimed to define the role of renal tubular epithelium-targeted PPAR-γ in protection of the epithelial phenotype and the antagonism of renal fibrosis and to define the underlying mechanisms...
September 1, 2016: Oncotarget
https://www.readbyqxmd.com/read/27432882/convergent-signaling-pathways-regulate-parathyroid-hormone-and-fibroblast-growth-factor-23-action-on-npt2a-mediated-phosphate-transport
#5
W Bruce Sneddon, Giovanni W Ruiz, Luciana I Gallo, Kunhong Xiao, Qiangmin Zhang, Youssef Rbaibi, Ora A Weisz, Gerard L Apodaca, Peter A Friedman
Parathyroid hormone (PTH) and FGF23 are the primary hormones regulating acute phosphate homeostasis. Human renal proximal tubule cells (RPTECs) were used to characterize the mechanism and signaling pathways of PTH and FGF23 on phosphate transport and the role of the PDZ protein NHERF1 in mediating PTH and FGF23 effects. RPTECs express the NPT2A phosphate transporter, αKlotho, FGFR1, FGFR3, FGFR4, and the PTH receptor. FGFR1 isoforms are formed from alternate splicing of exon 3 and of exon 8 or 9 in Ir-like loop 3...
September 2, 2016: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/27409796/mesenchymal-stromal-cells-epithelial-transition-induced-by-renal-tubular-cells-derived-extracellular-vesicles
#6
Giulia Chiabotto, Stefania Bruno, Federica Collino, Giovanni Camussi
Mesenchymal-epithelial interactions play an important role in renal tubular morphogenesis and in maintaining the structure of the kidney. The aim of this study was to investigate whether extracellular vesicles (EVs) produced by human renal proximal tubular epithelial cells (RPTECs) may induce mesenchymal-epithelial transition of bone marrow-derived mesenchymal stromal cells (MSCs). To test this hypothesis, we characterized the phenotype and the RNA content of EVs and we evaluated the in vitro uptake and activity of EVs on MSCs...
2016: PloS One
https://www.readbyqxmd.com/read/27186953/anti-diabetic-agent-sodium-tungstate-induces-the-secretion-of-pro-and-anti-inflammatory-cytokines-by-human-kidney-cells
#7
Romina Bertinat, Francisco Westermeier, Pamela Silva, Jie Shi, Francisco Nualart, Xuhang Li, Alejandro J Yáñez
Diabetic kidney disease (DKD) is the major cause of end stage renal disease. Sodium tungstate (NaW) exerts anti-diabetic and immunomodulatory activities in diabetic animal models. Here, we used primary cultures of renal proximal tubule epithelial cells derived from type-2-diabetic (D-RPTEC) and non-diabetic (N-RPTEC) subjects as in vitro models to study the effects of NaW on cytokine secretion, as these factors participate in intercellular regulation of inflammation, cell growth and death, differentiation, angiogenesis, development and repair, all processes that are dysregulated during DKD...
May 17, 2016: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/26981789/the-protective-role-of-g-protein-coupled-estrogen-receptor-1-gper-1-on-methotrexate-induced-nephrotoxicity-in-human-renal-epithelium-cells
#8
Akif Hakan Kurt, Fulsen Bozkus, Nuray Uremis, Muhammed Mehdi Uremis
Nephrotoxicity is an important problem during methotrexate (MTX) treatment, which has been widely used for the treatment of several cancer types. Females are less susceptible to kidney diseases; however, the reason for this condition has not to be fully clarified. But sex hormones such as estrogen may have a protective effect on the kidney. We aimed to evaluate the possible protective role of estrogen on the MTX-induced renal epithelial cell death. Primary renal proximal tubular epithelial cells (RPTEC) were incubated with MTX (1, 10 and 100 μM), either alone or in combination with the 17β-estradiol, G protein-coupled estrogen receptor 1 (GPER1) agonist G-1, estrogen receptor alpha agonist propyl pyrazole triol (PPT), estrogen receptor beta agonist diarylpropionitrile (DPN)...
June 2016: Renal Failure
https://www.readbyqxmd.com/read/26925784/overexpression-of-btg2-suppresses-growth-migration-and-invasion-of-human-renal-carcinoma-cells-in-vitro
#9
Jin Sima, B Zhang, X Y Sima, Y X Mao
The objective of the study was to investigate the impact of BTG2 on growth, migration and invasion of human clear cell renal cell carcinoma (ccRCC) cells. Endogenous expression of BTG2 was evaluated in the ccRCC cell lines (Caki-1, 786-O and Caki-2) and noncancerous human renal proximal tubular cell lines (HKC, HK-2 and RPTEC). BTG2 expression was decreased in the ccRCC cells compared with the noncancerous cells (P < 0.01). Then Caki-1 and 786-O cells described as suitable transfection hosts were used in transfection to carry out biological function studies...
2016: Neoplasma
https://www.readbyqxmd.com/read/26908771/metallothioneins-and-renal-ageing
#10
Johannes Leierer, Michael Rudnicki, Susie-Jane Braniff, Paul Perco, Christian Koppelstaetter, Irmgard Mühlberger, Susanne Eder, Julia Kerschbaum, Christoph Schwarzer, Andrea Schroll, Günter Weiss, Stefan Schneeberger, Silvia Wagner, Alfred Königsrainer, Georg A Böhmig, Gert Mayer
BACKGROUND: Human lifespan is increasing continuously and about one-third of the population >70 years of age suffers from chronic kidney disease. The pathophysiology of the loss of renal function with ageing is unclear. METHODS: We determined age-associated gene expression changes in zero-hour biopsies of deceased donor kidneys without laboratory signs of impaired renal function, defined as a last serum creatinine >0.96 mg/dL in females and >1.18 mg/dL in males, using microarray technology and the Significance Analysis of Microarrays routine...
September 2016: Nephrology, Dialysis, Transplantation
https://www.readbyqxmd.com/read/26661279/sglt2-inhibitors-glucotoxicity-and-tumorigenesis-downstream-the-renal-proximal-tubule
#11
REVIEW
Romina Bertinat, Francisco Nualart, Alejandro J Yáñez
At present, diabetes mellitus is the main cause of end-stage renal disease. Effective glycaemic management is the most powerful tool to delay the establishment of diabetic complications, such as diabetic kidney disease. Together with reducing blood glucose levels, new anti-diabetic agents are expected not only to control the progression but also to restore known defects of the diabetic kidney. Sodium-glucose co-transporter 2 (SGLT2) inhibitors are promising anti-diabetic agents that reduce hyperglycaemia by impairing glucose reabsorption in proximal tubule of the kidney and increasing glucosuria...
August 2016: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/26362508/biokinetics-in-repeated-dosing-in-vitro-drug-toxicity-studies
#12
Nynke I Kramer, Emma Di Consiglio, Bas J Blaauboer, Emanuela Testai
The aim of the EU FP7 Predict-IV project was to improve the predictivity of in vitro assays for unwanted effects of drugs after repeated dosing. The project assessed the added benefit of integrating long-lived in vitro organotypic cell systems with 'omics' technologies and in silico modelling, including systems biology and pharmacokinetic assessments. RPTEC/TERT1 kidney cells, primary rat and human hepatocytes, HepaRG liver cells and 2D and 3D primary brain cultures were dosed daily or every other day for 14 days to a selection of drugs varying in their mechanism of pharmacological action...
December 25, 2015: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/26209740/new-developments-concerning-the-proximal-tubule-in-diabetic-nephropathy-in-vitro-models-and-mechanisms
#13
REVIEW
Jennifer Slyne, Craig Slattery, Tara McMorrow, Michael P Ryan
The incidence of Type 2 diabetes is increasing rapidly worldwide, and understanding the mechanisms of its complications including diabetic nephropathy (DN) is important in the discovery of early biomarkers, understanding the causative mechanisms of its complications and identifying therapeutic targets. DN is characterized by glomerulosclerosis, tubulointerstitial fibrosis and tubular atrophy. The tubular component of the disease is important in progression of disease. In vitro models are a valuable alternative to animal studies and an effective way to explore mechanisms of human disease...
August 2015: Nephrology, Dialysis, Transplantation
https://www.readbyqxmd.com/read/26152359/human-renal-normal-tumoral-and-cancer-stem-cells-express-membrane-bound-interleukin-15-isoforms-displaying-different-functions
#14
COMPARATIVE STUDY
Sandy Azzi, Cindy Gallerne, Cristina Romei, Vincent Le Coz, Rosaria Gangemi, Krystel Khawam, Aurore Devocelle, Yanhong Gu, Stefania Bruno, Silvano Ferrini, Salem Chouaib, Pierre Eid, Bruno Azzarone, Julien Giron-Michel
Intrarenal interleukin-15 (IL-15) participates to renal pathophysiology, but the role of its different membrane-bound isoforms remains to be elucidated. In this study, we reassess the biology of membrane-bound IL-15 (mb-IL-15) isoforms by comparing primary cultures of human renal proximal tubular epithelial cells (RPTEC) to peritumoral (ptumTEC), tumoral (RCC), and cancer stem cells (CSC/CD105(+)). RPTEC express a 14 to 16 kDa mb-IL-15, whose existence has been assumed but never formally demonstrated and likely represents the isoform anchored at the cell membrane through the IL-15 receptor α (IL-15Rα) chain, because it is sensitive to acidic treatment and is not competent to deliver a reverse signal...
June 2015: Neoplasia: An International Journal for Oncology Research
https://www.readbyqxmd.com/read/26062793/chrysin-inhibits-diabetic-renal-tubulointerstitial-fibrosis-through-blocking-epithelial-to-mesenchymal-transition
#15
Min-Kyung Kang, Sin-Hye Park, Yean-Jung Choi, Daekeun Shin, Young-Hee Kang
UNLABELLED: Renal fibrosis is a crucial event in the pathogenesis of diabetic nephropathy (DN). The process known as epithelial to mesenchymal transition (EMT) contributes to the accumulation of matrix proteins in kidneys, in which renal tubular epithelial cells play an important role in progressive renal fibrosis. The current study investigated that chrysin (5,7-dihydroxyflavone) present in bee propolis and herbs, inhibited renal tubular EMT and tubulointerstitial fibrosis due to chronic hyperglycemia...
July 2015: Journal of Molecular Medicine: Official Organ of the "Gesellschaft Deutscher Naturforscher und Ärzte"
https://www.readbyqxmd.com/read/26055719/nephron-toxicity-profiling-via-untargeted-metabolome-analysis-employing-a-high-performance-liquid-chromatography-mass-spectrometry-based-experimental-and-computational-pipeline
#16
Christina Ranninger, Marc Rurik, Alice Limonciel, Silke Ruzek, Roland Reischl, Anja Wilmes, Paul Jennings, Philip Hewitt, Wolfgang Dekant, Oliver Kohlbacher, Christian G Huber
Untargeted metabolomics has the potential to improve the predictivity of in vitro toxicity models and therefore may aid the replacement of expensive and laborious animal models. Here we describe a long term repeat dose nephrotoxicity study conducted on the human renal proximal tubular epithelial cell line, RPTEC/TERT1, treated with 10 and 35 μmol·liter(-1) of chloroacetaldehyde, a metabolite of the anti-cancer drug ifosfamide. Our study outlines the establishment of an automated and easy to use untargeted metabolomics workflow for HPLC-high resolution mass spectrometry data...
July 31, 2015: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/25871823/et-1-induced-downregulation-of-mrp2-via-mirna-133a-a-marker-for-tubular-nephrotoxicity
#17
Heike Loeser, Melanie von Brandenstein, Angela Herschung, Monika Schlosser, Reinhard Büttner, Jochen W U Fries
BACKGROUND: Multiple drug resistance (MDR), known from treating malignant tumors with chemotherapy, increases the efflux of reabsorbed reagents in tumor cells. This mechanism has been reported in the renal proximal tubule and may prevent therapeutic tubular protection in proteinuria. Since endothelin-1 (ET-1), a major component in the urine of proteinuric patients, stimulates proximal tubules, its influence on MDR was analyzed with emphasis on the multidrug resistance-associated protein 2 (MRP2), a prominent transporter in the human proximal tubule and microRNA (miRNA) 133a...
2015: American Journal of Nephrology
https://www.readbyqxmd.com/read/25796475/optimization-of-a-quantitative-pcr-based-method-for-plasmid-copy-number-determination-in-human-cell-lines
#18
Lukas Fliedl, Florian Kast, Johannes Grillari, Matthias Wieser, Regina Grillari-Voglauer
Transient gene expression (TGE) is an essential tool for the production of recombinant proteins, especially in early drug discovery and development phases of biopharmaceuticals. The need for fast production of sufficient recombinant protein for initial tests has dramatically increased with increase in the identification of potential novel pharmaceutical targets. One of the critical factors for transient transfection is plasmid copy number (PCN), for which we here provide an optimized qPCR based protocol. Thereby, we show the loss of PCN during a typical batch process of HEK293 cells after transfection from 606,000 to 4560 copies per cell within 5 days...
December 25, 2015: New Biotechnology
https://www.readbyqxmd.com/read/25649790/characterization-of-the-interaction-of-staphylococcal-enterotoxin-b-with-cd1d-expressed-in-human-renal-proximal-tubule-epithelial-cells
#19
Rasha Hammamieh, Nabarun Chakraborty, Yixin Lin, Jeffrey W Shupp, Stacy-Ann Miller, Sam Morris, Marti Jett
BACKGROUND: Participation of renal cells in the pathogenesis of staphylococcal enterotoxin B (SEB) is critical for late cleansing and sequestration of the antigens facilitated by CD1d mediated antigen sensing and recognition. This is a noted deviation from the typical antigen recognition process that recruits the major histocompatibility complex class II (MHC II) molecules. The immunological importance of CD1d is underscored by its influences on the performances of natural killer T-cells and thereby mediates the innate and adaptive immune systems...
2015: BMC Microbiology
https://www.readbyqxmd.com/read/25500123/expression-of-xenobiotic-transporters-in-the-human-renal-proximal-tubule-cell-line-rptec-tert1
#20
Lydia Aschauer, Giada Carta, Nadine Vogelsang, Eberhard Schlatter, Paul Jennings
The kidney is a major target for drug-induced injury, primarily due the fact that it transports a wide variety of chemical entities into and out of the tubular lumen. Here, we investigated the expression of the main xenobiotic transporters in the human renal proximal tubule cell line RPTEC/TERT1 at an mRNA and/or protein level. RPTEC/TERT1 cells expressed OCT2, OCT3, OCTN2, MATE1, MATE2, OAT1, OAT3 and OAT4. The functionality of the OCTs was demonstrated by directional transport of the fluorescent dye 4-Di-1-ASP...
December 25, 2015: Toxicology in Vitro: An International Journal Published in Association with BIBRA
keyword
keyword
28678
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"