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https://www.readbyqxmd.com/read/27924544/multiplex-detection-of-aspergillus-species
#1
Pedro Martínez-Culebras, María Victoria Selma, Rosa Aznar
Multiplex real-time polymerase chain reaction (PCR) provides a fast and accurate DNA-based tool for the simultaneous amplification of more than one target sequence in a single reaction. Here a duplex real-time PCR assay is described for the simultaneous detection of Aspergillus carbonarius and members of the Aspergillus niger aggregate, which are the main responsible species for ochratoxin A (OTA) contamination in grapes. This single tube reaction targets the beta-ketosynthase and the acyl transferase domains of the polyketide synthase of A...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27921330/the-new-face-of-isotopic-nmr-at-natural-abundance
#2
Tangi Jézéquel, Valentin Joubert, Patrick Giraudeau, Gérald S Remaud, Serge Akoka
The most widely used method for isotope analysis at natural abundance is isotope ratio monitoring by Mass Spectrometry (irm-MS) which provides bulk isotopic composition in (2) H, (13) C, (15) N, (18) O or (34) S. However, in the 1980s, the direct access to Site-specific Natural Isotope Fractionation by Nuclear Magnetic Resonance (SNIF-NMR(TM) ) was immediately recognized as a powerful technique to authenticate the origin of natural or synthetic products. The initial - and still most popular - application consisted in detecting the chaptalization of wines by irm-(2) H NMR...
November 7, 2016: Magnetic Resonance in Chemistry: MRC
https://www.readbyqxmd.com/read/27918105/resveratrol-inhibits-proliferation-invasion-and-epithelial-mesenchymal-transition-by-increasing-mir-200c-expression-in-hct-116-colorectal-cancer-cells
#3
Fatemeh Karimi Dermani, Massoud Saidijam, Razieh Amini, Ali Mahdavinezhad, Korosh Heydari, Rezvan Najafi
OBJECTIVE: Colorectal cancer (CRC) is one of the most common malignancies, associated with high rates of relapse. A notable challenge in treatment is low response rate to current therapies for advanced CRC. The miR-200c plays an essential role in tumor suppression by inhibiting epithelial-mesenchymal transition (EMT). Resveratrol, a natural compound found in red wine, reveals anti-cancer properties in several types of cancers such as CRC. The aim of current study was to evaluate the effects of resveratrol on proliferation, apoptosis and invasion of HCT-116 cells and also expression of EMT-related genes in presences or absence of miR-200c...
December 5, 2016: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/27916898/a-multi-pumping-flow-system-for-in-situ-measurements-of-dissolved-manganese-in-aquatic-systems
#4
David Meyer, Ralf D Prien, Olaf Dellwig, Joanna J Waniek, Ingo Schuffenhauer, Jan Donath, Siegfried Krüger, Malte Pallentin, Detlef E Schulz-Bull
A METals In Situ analyzer (METIS) has been used to determine dissolved manganese (II) concentrations in the subhalocline waters of the Gotland Deep (central Baltic Sea). High-resolution in situ measurements of total dissolved Mn were obtained in near real-time by spectrophotometry using 1-(2-pyridylazo)-2-naphthol (PAN). PAN is a complexing agent of dissolved Mn and forms a wine-red complex with a maximum absorbance at a wavelength of 562 nm. Results are presented together with ancillary temperature, salinity, and dissolved O 2 data...
November 30, 2016: Sensors
https://www.readbyqxmd.com/read/27916747/the-broadly-tuned-odorant-receptor-or1a1-is-highly-selective-for-3-methyl-2-4-nonanedione-a-key-food-odorant-in-aged-wines-tea-and-other-foods
#5
Christiane Geithe, Franziska Noe, Johanna Kreissl, Dietmar Krautwurst
Key food odorants are the most relevant determinants by which we detect, recognize, and hedonically evaluate the aroma of foods and beverages. Odorants are detected by our chemical sense of olfaction, comprising a set of approximately 400 different odorant receptor types. However, the specific receptor activity patterns representing the aroma percepts of foods or beverages, as well as the key food odorant agonist profiles of single-odorant receptors, are largely unknown. We aimed to establish comprehensive key food odorant agonist profiles of 2 unrelated, broadly tuned receptors, OR1A1 and OR2W1, that had been associated thus far with mostly non-key food odorants and shared some of these agonists...
December 4, 2016: Chemical Senses
https://www.readbyqxmd.com/read/27916121/development-of-an-indirect-elisa-for-the-determination-of-ethyl-carbamate-in-chinese-rice-wine
#6
Lin Luo, Hong-Tao Lei, Jin-Yi Yang, Gong-Liang Liu, Yuan-Ming Sun, Wei-Dong Bai, Hong Wang, Yu-Dong Shen, Sui Chen, Zhen-Lin Xu
The widespread occurrence of ethyl carbamate (EC, 89.09 Da), a group 2A carcinogen, in fermented foods and alcoholic beverages has raised worldwide public health concern. Immunoassay for EC is unavailable due to the simple and small structure of EC. In this work, an initial attempt to produce antibody specific for EC, by using 4-((ethoxycarbonyl)amino)butanoic acid as hapten, was made but failed. However, since EC can easily react with 9-xanthydrol to form xanthyl ethyl carbamate (XEC), two haptens based on XEC structure were designed and synthesized...
January 15, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/27915238/pontine-changes-in-metabolites-and-axonal-fibres-of-rats-following-four-week-alcohol-exposure-in-vivo-diffusion-tensor-imaging-and-1h-magnetic-resonance-spectroscopy-study-at-7-0-t
#7
Jianming Luo, Zhiwei Shen, Guang Chen, Dian Wang, Xiaojun Yu
AIMS: We characterized the metabolite content and axonal fibre changes in the rat pontine, in vivo, using (1)H-magnetic resonance spectroscopy (1H-MRS) and diffusion tensor imaging (DTI) with a 7.0 T magnetic resonance imaging (MRI) scanner following chronic alcohol administration to explore new indicators for the early diagnosis and treatment of chronic alcoholism. METHODS: Forty male Sprague-Dawley rats were randomly divided into the EtOH and control groups (water group)...
December 3, 2016: Alcohol and Alcoholism: International Journal of the Medical Council on Alcoholism
https://www.readbyqxmd.com/read/27913851/impact-of-oxygenation-on-the-performance-of-three-non-saccharomyces-yeasts-in-co-fermentation-with-saccharomyces-cerevisiae
#8
Kirti Shekhawat, Florian F Bauer, Mathabatha E Setati
The sequential or co-inoculation of grape must with non-Saccharomyces yeast species and Saccharomyces cerevisiae wine yeast strains has recently become a common practice in winemaking. The procedure intends to enhance unique aroma and flavor profiles of wine. The extent of the impact of non-Saccharomyces strains depends on their ability to produce biomass and to remain metabolically active for a sufficiently long period. However, mixed-culture wine fermentations tend to become rapidly dominated by S. cerevisiae, reducing or eliminating the non-Saccharomyces yeast contribution...
December 2, 2016: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/27909090/alcohol-intake-and-risk-of-incident-melanoma-a-pooled-analysis-of-three-prospective-studies-in-the-united-states
#9
Andrew Rivera, Hongmei Nan, Tricia Li, Abrar Qureshi, Eunyoung Cho
BACKGROUND: Alcohol consumption is associated with increased risk of numerous cancers, but existing evidence for an association with melanoma is equivocal. No study has evaluated the association with different anatomic locations of melanoma. METHODS: We used data from three large prospective cohort studies to investigate whether alcohol intake was associated with risk of melanoma. Alcohol intake was assessed repeatedly by food-frequency questionnaires. A Cox proportional hazards model was used to calculate multivariate-adjusted hazard ratios (HRs)...
December 2016: Cancer Epidemiology, Biomarkers & Prevention
https://www.readbyqxmd.com/read/27906789/radon-and-thoron-in-air-occupational-exposure-study-within-selected-wine-cellars-of-the-western-cape-south-africa-and-associated-annual-effective-doses
#10
R Botha, R T Newman, R Lindsay, P P Maleka
This is the first known study of exposure of Rn (radon) and secondarily Rn (thoron) in-air activity concentrations assessed within nine selected wine cellars in four wine districts of the Western Cape (South Africa) and the associated annual occupational effective doses. E-PERM electret ion chambers (EIC) and RAD-7 α-detectors were used to perform these measurements. The radon in-air levels ranged from 12 ± 4 Bq m to 770 ± 40 Bq m within the nine selected wine cellars. Eight of the nine wine cellars (excluding results from cellar w-6) had a median radon in-air activity concentration of 48 ± 8 Bq m...
January 2017: Health Physics
https://www.readbyqxmd.com/read/27905961/estimating-average-alcohol-consumption-in-the-population-using-multiple-sources-the-case-of-spain
#11
Luis Sordo, Gregorio Barrio, María J Bravo, Joan R Villalbí, Albert Espelt, Montserrat Neira, Enrique Regidor
BACKGROUND: National estimates on per capita alcohol consumption are provided regularly by various sources and may have validity problems, so corrections are needed for monitoring and assessment purposes. Our objectives were to compare different alcohol availability estimates for Spain, to build the best estimate (actual consumption), characterize its time trend during 2001-2011, and quantify the extent to which other estimates (coverage) approximated actual consumption. METHODS: Estimates were: alcohol availability from the Spanish Tax Agency (Tax Agency availability), World Health Organization (WHO availability) and other international agencies, self-reported purchases from the Spanish Food Consumption Panel, and self-reported consumption from population surveys...
June 2, 2016: Population Health Metrics
https://www.readbyqxmd.com/read/27905883/carbohydrate-metabolism-in-oenococcus-oeni-a-genomic-insight
#12
Alice Cibrario, Claire Peanne, Marine Lailheugue, Hugo Campbell-Sills, Marguerite Dols-Lafargue
BACKGROUND: Oenococcus oeni is the bacterial species that drives malolactic fermentation in most wines. Several studies have described a high intraspecific diversity regarding carbohydrate degradation abilities but the link between the phenotypes and the genes and metabolic pathways has been poorly described. RESULTS: A collection of 41 strains whose genomic sequences were available and representative of the species genomic diversity was analyzed for growth on 18 carbohydrates relevant in wine...
December 1, 2016: BMC Genomics
https://www.readbyqxmd.com/read/27905092/broad-spectrum-of-antimicrobial-properties-of-commercial-wines-from-different-vitis-vinifera-l-varieties
#13
Aleksandra Radovanović, Biljana Arsić, Vladimir Radovanović, Branimir Jovančičević, Vesna Nikolić
Fifteen commercial wines produced from international and autochthonic varieties of Vitis vinifera L. cultivation of different Balkan winegrowing subregions were studied for their antimicrobial activity against six Gram-positive (Clostridium perfringens, Bacillus subtilis, Staphylococcus aureus, Listeria inocua, Sarcina lutea and Micrococcus flavus) and six Gram-negative (Escherichia coli, Pseudomonas aeruginosa, Salmonella enteritidis, Shigella sonnei, Klebsiella pneumonia and Proteus vulgaris) bacteria. The concentrations and types of phenolic compounds responsible for antibacterial activity in wines were investigated by HPLC and spectroscopic methods...
January 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/27904404/phenolic-composition-antioxidant-capacity-and-in-vitro-cytotoxicity-assessment-of-fruit-wines
#14
Ana Ljevar, Natka Ćurko, Marina Tomašević, Kristina Radošević, Višnja Gaurina Srček, Karin Kovačević Ganić
Fruit wines contain a wide range of phenolic compounds with biological effects, but their composition and potential benefits to human health have been studied to the much lesser extent compared to grape wines. The aim of this research is to study the phenolic profile of different types of fruit wines and to evaluate their antioxidant and biological potential. Commercially available fruit wines from blackberry, cherry, raspberry, blackcurrant, strawberry and apple produced in Croatia were analyzed. To the best of our knowledge, this study represents the first comprehensive screening of Croatian fruit wines...
June 2016: Food Technology and Biotechnology
https://www.readbyqxmd.com/read/27904403/quality-and-composition-of-air%C3%A3-n-wines-fermented-by-sequential-inoculation-of-lachancea-thermotolerans-and-saccharomyces-cerevisiae
#15
Ángel Benito, Fernando Calderón, Felipe Palomero, Santiago Benito
This study evaluates the influence of Lachancea thermotolerans on low-acidity Airén grape must from the south of Spain. For this purpose, combined fermentations with Lachancea thermotolerans and Saccharomyces cerevisiae were compared to a single fermentation by S. cerevisiae. Results of all developed analyses showed significant differences in several parameters including acidity, population growth kinetics, concentration of amino acids, volatile and non-volatile compounds, and sensorial parameters. The Airén wine quality increased mainly due to the acidification by L...
June 2016: Food Technology and Biotechnology
https://www.readbyqxmd.com/read/27903630/genetic-causes-of-phenotypic-adaptation-to-the-second-fermentation-of-sparkling-wines-in-saccharomyces-cerevisiae
#16
Maria Martí-Raga, Emilien Peltier, Albert Mas, Gemma Beltran, Philippe Marullo
Hybridization is known to improve complex traits due to heterosis and phenotypic robustness. However, these phenomena have been rarely explained at the molecular level. Here, the genetic determinism of Saccharomyces cerevisiae fermentation performance was investigated using a QTL-mapping approach on an F1-progeny population. Three main QTLs were detected, with positive alleles coming from both parental strains. The heterosis effect found in the hybrid was partially explained by three loci showing pseudo-overdominance and dominance effect...
November 30, 2016: G3: Genes—Genomes—Genetics
https://www.readbyqxmd.com/read/27902771/piceatannol-attenuates-renal-fibrosis-induced-by-unilateral-ureteral-obstruction-via-downregulation-of-histone-deacetylase-4-5-or-p38-mapk-signaling
#17
Sin Young Choi, Zhe Hao Piao, Li Jin, Jung Ha Kim, Gwi Ran Kim, Yuhee Ryu, Ming Quan Lin, Hyung-Seok Kim, Hae Jin Kee, Myung Ho Jeong
Piceatannol, a resveratrol metabolite, is a phenolic compound found in red wine and grapes. We investigated the effect of piceatannol on renal fibrosis and histone deacetylase (HDAC) expression in a mouse model of unilateral ureteral obstruction (UUO). Fibrosis was established by UUO and piceatannol was intraperitoneally injected for 2 weeks. Piceatannol suppressed extracellular matrix (ECM) protein deposition including collagen type I and fibronectin as well as connective tissue growth factor (CTGF) and α-smooth muscle actin (α-SMA) in UUO kidneys...
2016: PloS One
https://www.readbyqxmd.com/read/27901205/six-month-color-change-and-water-sorption-of-9-new-generation-flowable-composites-in-6-staining-solutions
#18
Maria Arregui, Luis Giner, Marco Ferrari, Marta Vallés, Montserrat Mercadé
Color match and water sorption are two factors that affect restorative materials. Discoloration is essential in the lifespan of restorations. The aim of this study was to evaluate color change and water sorption of nine flowable composites at multiple time points over 6 months. 60 samples of each composite were divided into two groups (Color Change and Water Sorption/Solubility). Each Color Change group was divided into six subgroups, which were immersed in distilled water (DW), coffee (CF), Coca-Cola (CC), red wine (RW), tea (TE) and orange juice (OJ)...
November 28, 2016: Brazilian Oral Research
https://www.readbyqxmd.com/read/27901083/resveratrol-serves-as-a-protein-substrate-interaction-stabilizer-in-human-sirt1-activation
#19
Xuben Hou, David Rooklin, Hao Fang, Yingkai Zhang
Resveratrol is a natural compound found in red wine that has been suggested to exert its potential health benefit through the activation of SIRT1, a crucial member of the mammalian NAD(+)-dependent deacetylases. SIRT1 has emerged as an attractive therapeutic target for many aging related diseases, however, how its activity can only be activated toward some specific substrates by resveratrol has been poorly understood. Herein, by employing extensive molecular dynamics simulations as well as fragment-centric topographical mapping of binding interfaces, we have clarified current controversies in the literature and elucidated that resveratrol plays an important activation role by stabilizing SIRT1/peptide interactions in a substrate-specific manner...
November 30, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27901076/prevalence-of-metabolic-syndrome-and-its-risk-factors-among-rural-adults-in-nantong-china
#20
Jing Xiao, Chuan-Li Wu, Yue-Xia Gao, Shu-Lan Wang, Lei Wang, Qing-Yun Lu, Xiao-Jian Wang, Tian-Qi Hua, Huan Shen, Hui Cai
The prevalence of metabolic syndrome (MS) varies worldwide due to genetic and environmental factors. A population-based cross-sectional study, with 37,582 participants recruited in Nantong, China in 2007-2008 (stage I) and 2013 (stage II). Socio-demographic, lifestyle factors, disease history and fasting blood sample were collected. The prevalence of MS was much higher in 2013 (42.6%) than that in 2007-2008 (21.6%), which was significantly higher in older people in both stages. Participants with two or more familial history of diseases were associated with a higher MS prevalence compared to those who didn't have familial history of diseases...
November 30, 2016: Scientific Reports
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