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https://www.readbyqxmd.com/read/28926682/development-of-a-simple-and-valid-method-for-the-trace-determination-of-phthalate-esters-in-human-plasma-using-dispersive-liquid-liquid-microextraction-coupled-with-gc-ms
#1
Karim Ebrahim, Parinaz Poursafa, Mohammad Mehdi Amin
A new method was developed for the trace determination of phthalic acid esters in plasma using dispersive liquid-liquid microextraction and gas chromatography with mass spectrometry analysis. Plasma proteins were efficiently precipitated by trichloroacetic acid and then a mixture of chlorobenzene (as extraction solvent) and acetonitrile (as dispersive solvent) rapidly injected to clear supernatant using a syringe. After the centrifuging, chlorobenzene were sedimented in the bottom of the test tube. 1 μL of this sedimented phase was injected into the gas chromatograph for phthalic acid esters analysis...
September 19, 2017: Journal of Separation Science
https://www.readbyqxmd.com/read/28926207/seasonal-rather-than-spatial-variability-drives-planktonic-and-benthic-bacterial-diversity-in-a-microtidal-lagoon-and-the-adjacent-open-sea
#2
G M Quero, L Perini, G Pesole, C Manzari, C Lionetti, M Bastianini, M Marini, G M Luna
Coastal lagoons are highly productive ecosystems, which are experiencing a variety of human disturbances at increasing frequency. Bacteria are key ecological players within lagoons, yet little is known about the magnitude, patterns and drivers of diversity in these transitional environments. We carried out a seasonal study in the Venice Lagoon (Italy) and the adjacent sea, to simultaneously explore diversity patterns in different domains (pelagic, benthic) and their spatio-temporal variability, and test the role of environmental gradients in structuring assemblages...
September 19, 2017: Molecular Ecology
https://www.readbyqxmd.com/read/28926205/prevalence-and-correlates-of-sleep-disturbance-among-older-women-in-vietnam
#3
Tiet-Hanh Dao-Tran, Charrlotte Seib
AIMS AND OBJECTIVES: To explore prevalence and correlates of sleep disturbance among women aged 60 and over in Vietnam. BACKGROUND: Sleep disturbance can negatively influence human's health. Sleep disturbance is likely to increase with age, and women appear to be more likely to experience sleep disturbance than men. Knowledge about sleep disturbance in women aged 60 and over in Vietnam is under-researched. DESIGN: The study presents results from a cross-sectional survey of 440 women aged 60 and over from 16 rural and urban suburbs in Vietnam from 8/2014 to 1/2015...
September 19, 2017: Journal of Clinical Nursing
https://www.readbyqxmd.com/read/28926189/environmental-peer-pressure-cd4-t-cell-help-in-tolerance-and-transplantation
#4
REVIEW
Dana Tedesco, Arash Grakoui
The liver participates in a multitude of metabolic functions that are critical for sustaining human life. Despite constant encounters with antigenic-rich intestinal blood, oxidative stress and metabolic intermediates, there is no appreciable immune response. Interestingly, patients undergoing orthotopic liver transplantation benefit from a high rate of graft acceptance in comparison to other solid organ transplant recipients. In fact, co-transplantation of a donor liver in tandem with a rejection-prone graft increases the likelihood of graft acceptance...
September 19, 2017: Liver Transplantation
https://www.readbyqxmd.com/read/28926158/selection-on-a-behaviour-related-gene-during-the-first-stages-of-the-biological-invasion-pathway
#5
J C Mueller, P Edelaar, A Baños-Villalba, M Carrete, J Potti, J Blas, J L Tella, B Kempenaers
Human-induced biological invasions are common worldwide and often have negative impacts on wildlife and human societies. Several studies have shown evidence for selection on invaders after introduction to the new range. However, selective processes already acting prior to introduction have been largely neglected. Here we tested whether such early selection acts on known behaviour-related gene variants in the yellow-crowned bishop (Euplectes afer), a pet-traded African songbird. We tested for non-random allele frequency changes after trapping, acclimation and survival in captivity...
September 19, 2017: Molecular Ecology
https://www.readbyqxmd.com/read/28926155/idna-screening-disease-vectors-as-vertebrate-samplers
#6
Arthur Kocher, Benoit De Thoisy, François Catzeflis, Sophie Valiere, Anne-Laure Bañuls, Jérôme Murienne
In the current context of global change and human-induced biodiversity decline, there is an urgent need for developing sampling approaches able to accurately describe the state of biodiversity. Traditional surveys of vertebrate fauna involve time-consuming and skill-demanding field methods. Recently, the use of DNA derived from invertebrate parasites (leeches and blowflies) was suggested as a new tool for vertebrate diversity assessment. Blood meal analyses of arthropod disease vectors have long been performed to describe their feeding behaviour, for epidemiological purposes...
September 19, 2017: Molecular Ecology
https://www.readbyqxmd.com/read/28926141/working-at-the-membrane-interface-ligand-induced-changes-in-dynamic-conformation-and-oligomeric-structure-in-human-aromatase
#7
Giovanna Di Nardo, Giuseppe Cimicata, Roberta Baravalle, Valentina Dell'Angelo, Alberto Ciaramella, Gianluca Catucci, Piero Ugliengo, Gianfranco Gilardi
Aromatase catalyses the biosynthesis of estrogens from androgens. Due to the physiological importance of this conversion of lipophilic substrates, the interaction with the lipid bilayer for this cytochrome P450 is crucial for its dynamics that must allow an easy access to substrates and inhibitors. Here, aromatase-anastrozole interaction is studied combining computational methods to identify possible access/egress routes with the protein inserted in the membrane and experimental tools aimed at the investigation of the effect of the inhibitor on the protein conformation...
September 19, 2017: Biotechnology and Applied Biochemistry
https://www.readbyqxmd.com/read/28926138/tnfaip8-regulates-hippo-pathway-through-interacting-with-lats1-to-promote-cell-proliferation-and-invasion-in-lung-cancer
#8
Yong Han, ZhongPing Tang, Yue Zhao, Qingchang Li, Enhua Wang
TNFAIP8 is associated with prognosis of several human malignancies. However, the molecular mechanism of TNFAIP8 in lung cancer remains unknown. In our study, we found TNFAIP8 could enhance TEAD luciferase activity and inhibits the activity of Hippo pathway. TNFAIP8 also increased cyclin D1, CDK6 and decreased p27 in lung cancer cells. In addition, TNFAIP8 increased total YAP protein and promoted nuclear localization of YAP. More importantly, YAP depletion blocked the role of TNFAIP8 on cell cycle-related proteins and TEAD luciferase activity, revealing that TNFAIP8 regulates Hippo pathway in a YAP-dependend manner...
September 19, 2017: Molecular Carcinogenesis
https://www.readbyqxmd.com/read/28926119/procaine-is-a-specific-dna-methylation-inhibitor-with-anti-tumor-effect-for-human-gastric-cancer
#9
Yong-Chao Li, Yun Wang, Dan-Dan Li, Ying Zhang, Tian-Cheng Zhao, Chang-Feng Li
DNA hypermethylation and the silencing of tumor suppressor genes caused by DNA hypermethylation is considered as a molecular hallmark of many kinds of cancers. Procaine, a local anesthetic, has been shown as a potential DNA methylation inhibitor in some types of cancers. However, the influence of procaine on DNA methylation regulation as well as the biological function in gastric cancer is still unknown. We report here that procaine represses the DNA-methylation level and promotes the proliferation arrest and apoptosis of gastric cancer cells...
September 19, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28926118/chop-induced-loss-of-intestinal-epithelial-stemness-contributes-to-bile-duct-ligation-induced-cholestatic-liver-injury
#10
Runping Liu, Xiaojiaoyang Li, Zhiming Huang, Derrick Zhao, Bhagyalaxmi Sukka Ganesh, Guanhua Lai, William M Pandak, Phillip B Hylemon, Jasmohan S Bajaj, Arun J Sanyal, Huiping Zhou
Impaired intestinal barrier function promotes the progression of various liver diseases including cholestatic liver disease. The close association of primary sclerosing cholangitis (PSC) with inflammatory bowel disease highlights the importance of the gut-liver axis. It has been reported that bile duct ligation (BDL)-induced liver fibrosis is significantly reduced in C/EBP homologous protein knock out (CHOP(-/-) ) mice. However, the underlying mechanisms remain unclear. In the current study, we demonstrate that BDL induces striking and acute hepatic ER stress responses after 1 day, which return to normal after 3 days...
September 19, 2017: Hepatology: Official Journal of the American Association for the Study of Liver Diseases
https://www.readbyqxmd.com/read/28926110/pacap-and-pac1r-are-differentially-expressed-in-motor-cortex-of-amyotrophic-lateral-sclerosis-patients-and-support-survival-of-ipsc-derived-motor-neurons
#11
Gabriele Bonaventura, Rosario Iemmolo, Agata Grazia D'Amico, Valentina La Cognata, Erminio Costanzo, Mario Zappia, Velia D'Agata, Francesca Luisa Conforti, Eleonora Aronica, Sebastiano Cavallaro
Amyotrophic lateral sclerosis (ALS) is a fatal and disabling neurodegenerative disease characterized by upper and lower motor neurons depletion. In our previous work, comprehensive genomic profiling of 41 motor cortex samples enabled to discriminate controls from sporadic ALS patients, and segregated these latter into two distinct subgroups (SALS1 and SALS2), each associated with different deregulated genes. In the present study, we focused our attention on two of them, Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP) and its type 1 receptor (PAC1R), and validated the results of the transcriptome experiments by quantitative reverse transcription-polymerase chain reaction (qRT-PCR), immunohistochemistry and western blot analysis...
September 19, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28926109/role-of-viruses-in-gastrointestinal-cancer
#12
REVIEW
Habibollah Mirzaei, Hossein Goudarzi, Gita Eslami, Ebrahim Faghihloo
Gastrointestinal cancers are a global public health problem, which represent a vast majority of all cancer-caused deaths in both men and women. On the other hand, viral pathogens have been long implicated as etiological factors in the onset of certain human cancers, including gastrointestinal tumors. In this regard, Human Papilloma Virus (HPV), Epstein - Barr Virus (EBV) and John Cunningham Virus (JCV) have been more strongly suggested to be involved in gastrointestinal carcinogenesis; so that, the association of HPV with oropharyngeal and anal cancers and also the association of EBV with gastric cancer have been etiologically confirmed by epidemiological and experimental investigations...
September 19, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28926106/oroxyloside-inhibits-angiogenesis-through-suppressing-internalization-of-vegfr2-flk-1-in-endothelial-cells
#13
Kai Zhao, Xiaorui Li, Binyan Lin, Dawei Yang, Yuxin Zhou, Zhiyu Li, Qinglong Guo, Na Lu
Increasing flavonoids have been reported to possess anti-angiogenic effects. Inhibition of angiogenesis plays a critical role in the treatment of cancer, especially in advanced metastatic cancer. In this study, we assessed the effect of Oroxylin A-7-glucuronide (Oroxyloside), a main metabolite of Oroxylin A, on angiogenesis in human endothelial cell-like EA.hy926 cells. Oroxyloside suppressed the migration and tube formation of EA.hy926 cells. Meanwhile, microvessels sprouting from aortic rings and new blood vessels on the chicken chorioallantoic membrane (CAM) were also inhibited...
September 19, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28926093/pulmonary-hypertension-molecular-aspects-of-current-therapeutic-intervention-and-future-direction
#14
Taruna Katyal Arora, Amit KumarArora, Monika Katyal Sachdeva, Satyendra Kumar Rajput, Arun Kumar Sharma
Pulmonary hypertension (PH) is a life-threatening lung disorder with towering prevalence and risk for future has been gradually rising worldwide. Even, no specific medications are available for pulmonary hypertension; various classes of treatment based upon the origin and magnitude of hypertension are still used for the treatment of PH. Consideration of molecular or signaling modulation is the imperative approach that can offer a new notion for prevalent pharmacotherapeutic agents. Instead of concurrent targets, including endothelin receptor antagonists (ETA/ETB), phosphodiesterase 5 inhibitor (PDF-5), calcium channel blockers, anticoagulants, diuretics and long acting prostacyclin analogue, recent scientific reports revealed the numerous potential alternative therapeutic approaches that can significantly target the pathological signaling alteration associated with PH...
September 19, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28926092/key-genes-regulating-the-liposecretion-process-of-mature-adipocytes
#15
Giulia Maurizi, Tiina Petäistö, Angela Maurizi, Lucio Della Guardia
White mature adipocytes (MAs) are plastic cells able to reversibly transdifferentiate toward fibroblast-like cells maintaining stem cell gene signatures. The main morphologic aspect of this transdifferentiation process, called liposecretion, is the secretion of large lipid droplets and the development of organelles necessary for exocrine secretion. There is a considerable interest in the adipocyte plastic properties involving liposecretion process, but the molecular details are incompletely explored. This review analyzes the gene expression of MAs isolated from human subcutaneous fat tissue with respect to bone marrow (BM)-derived mesenchymal stem cells (MSC) focusing on gene regulatory pathways involved into cellular morphology changes, cellular proliferation and transports of molecules through the membrane, suggesting potential ways to guide liposecretion...
September 19, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28925592/defining-a-microrna-mrna-interaction-map-for-calcineurin-inhibitor-induced-nephrotoxicity
#16
Christopher J Benway, John Iacomini
Calcineurin inhibitors induce nephrotoxicity through poorly understood mechanisms thereby limiting their use in transplantation and other diseases. Here we define a microRNA (miRNA)-messenger RNA (mRNA) interaction map that facilitates exploration into the role of miRNAs in cyclosporine-induced nephrotoxicity (CIN) and the gene pathways they regulate. Using photoactivatable ribonucleoside-enhanced crosslinking and immunoprecipitation (PAR-CLIP), we isolated RNAs associated with Argonaute 2 in the RNA-Induced Silencing Complex (RISC) of cyclosporine A (CsA) treated and control human proximal tubule cells and identified mRNAs undergoing active targeting by miRNAs...
September 19, 2017: American Journal of Transplantation
https://www.readbyqxmd.com/read/28925571/human-factors-and-simulation-in-emergency-medicine
#17
Emily M Hayden, Ambrose H Wong, Jeremy Ackerman, Margaret K Sande, Charles Lei, Leo Kobayashi, Michael Cassara, Dylan D Cooper, Kimberly Perry, William E Lewandowski, Mark W Scerbo
This consensus group from the 2017 Academic Emergency Medicine Consensus Conference "Catalyzing System Change through Health Care Simulation: Systems, Competency, and Outcomes" held in Orlando, Florida on May 16, 2017 focused on the use of human factors and simulation in the field of emergency medicine. The human factors discipline is often underutilized within emergency medicine but has significant potential in improving the interface between technologies and individuals in the field. The discussion explored the domain of human factors, its benefits in medicine, how simulation can be a catalyst for human factors work in emergency medicine, and how emergency medicine can collaborate with human factors professionals to affect change...
September 19, 2017: Academic Emergency Medicine: Official Journal of the Society for Academic Emergency Medicine
https://www.readbyqxmd.com/read/28925558/endothelial-precursor-cell-crossmatch-using-tie-2-enriched-spleen-cells
#18
Volker Daniel, Caner Süsal, Sabine Scherer, Hien Tran, Petra Gombos, Karina Trojan, Mahmoud Sadeghi, Christian Morath, Gerhard Opelz
BACKGROUND: Non-HLA-antibodies against human endothelial progenitor cells (EPC) in pre-transplant recipient serum can have a deleterious influence on the graft. EPC enriched from peripheral blood have been commonly used for EPC crossmatching. In the present study, we describe crossmatches using EPC enriched from fresh or frozen-thawed spleen cell preparations, thereby widening the sample source for deceased-donor crossmatching and retrospective studies. METHODS: EPC crossmatches were performed retrospectively using spleen cells and the flow cytometric XM-ONE crossmatch test kit...
September 19, 2017: Clinical Transplantation
https://www.readbyqxmd.com/read/28925528/relatedness-of-wildlife-and-livestock-avian-isolates-of-the-nosocomial-pathogen-acinetobacter-baumannii-to-lineages-spread-in-hospitals-worldwide
#19
Gottfried Wilharm, Evelyn Skiebe, Paul G Higgins, Marie T Poppel, Ulrike Blaschke, Sarah Leser, Christine Heider, Magdalena Heindorf, Paul Brauner, Udo Jäckel, Karin Böhland, Christiane Cuny, Andżelina Łopińska, Piotr Kaminski, Mariusz Kasprzak, Marcin Bochenski, Olaf Ciebiera, Marcin Tobółka, Katarzyna M Żołnierowicz, Joachim Siekiera, Harald Seifert, Stéphanie Gagné, Suzana P Salcedo, Michael Kaatz, Franziska Layer, Jennifer K Bender, Stephan Fuchs, Torsten Semmler, Yvonne Pfeifer, Leszek Jerzak
The natural habitats and potential reservoirs of the nosocomial pathogen Acinetobacter baumannii are poorly defined. Here, we put forth and tested the hypothesis of avian reservoirs of A. baumannii. We screened tracheal and rectal swab samples from livestock (chicken, geese) and wild birds (white stork nestlings) and isolated A. baumannii from 3% of sampled chicken (n=220), 8% of geese (n=40) and 25% of white stork nestlings (n=661). Virulence of selected avian A. baumannii isolates was comparable to that of clinical isolates in the Galleria mellonella infection model...
September 19, 2017: Environmental Microbiology
https://www.readbyqxmd.com/read/28925524/hypothermia-increases-aquaporin-4-aqp4-plasma-membrane-abundance-in-human-primary-cortical-astrocytes-via-a-calcium-transient-receptor-potential-vanilloid-4-trpv4-and-calmodulin-mediated-mechanism
#20
Mootaz M Salman, Philip Kitchen, M Nicola Woodroofe, James E Brown, Roslyn M Bill, Alex C Conner, Matthew T Conner
Human aquaporin 4 (AQP4) is the primary water channel protein in brain astrocytes. Hypothermia is known to cause astrocyte swelling in culture, but the precise role of AQP4 in this process is unknown. Primary human cortical astrocytes were cultured under hypothermic (32°C) or normothermic (37°C) conditions. AQP4 transcript, total protein and surface localized protein were quantified using RT-qPCR, sandwich ELISA with whole cell lysates, or cell-surface biotinylation followed by ELISA analysis of the surface-localized protein, respectively...
September 19, 2017: European Journal of Neuroscience
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