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P selectin

B Sobolewska, J Golenko, S Poeschel, C Grimmel, A Gatsiou, K Sopova, T Biedermann, K Schenke-Layland, K Stellos, F Ziemssen
Aflibercept appears to accumulate in systemic circulation following intravitreal injections in therapy of neovascular age-related macular degeneration. This gives raise to the question of whether aflibercept affects platelets and their function such as activation and aggregation, which are substantial in the pathogenesis of an arterial thromboembolic event (ATE). In order to determine the effect of aflibercept in platelet activation, platelets from healthy volunteers were treated with aflibercept and its solvents at equal concentrations (0...
June 14, 2018: Experimental Eye Research
Punam P Parikh, Roberta M Lassance-Soares, Hongwei Shao, Manuela M Regueiro, Yan Li, Zhao-Jun Liu, Omaida C Velazquez
BACKGROUND: Poor wound healing in critical limb ischemia (CLI) is attributed to impaired neovascularization and reperfusion. Optimizing the ischemic wound with adhesion molecules that enhance stem cell homing may revolutionize treatment. The purpose of this study is to test the efficacy of adhesion molecule E-selectin on wound healing in an ischemic mouse wound. METHODS: Adult FVB/NJ mice underwent unilateral femoral artery and vein ligation to induce CLI. A 4-mm punch biopsy wound was created on the anterior thigh to simulate ischemic wounds...
August 2018: Journal of Surgical Research
Erin P Childers, Nathan Z Dreger, Alex B Ellenberger, Mary Beth Wandel, Karen Domino, Yanyi Xu, Derek Luong, Jiayi Yu, David Orsini, Robert H Bell, Christopher Premanandan, Stephen D Fening, Matthew L Becker
Platelet-rich plasma (PRP) is a clinically relevant source of growth factors used commonly by surgeons. The clinical efficacy of PRP use as reported in the literature is widely variable which is likely attributed to poorly defined retention time of PRP at the repair site. To overcome this limitation, branched poly(ester urea) (PEU) nanofibers were used to adsorb and retain PRP at the implant site in an acute rotator-cuff tear model in rats. The adsorption of PRP to branched-PEU was characterized using quartz crystal microbalance (QCM) and immuno-protein assay...
June 15, 2018: Biomacromolecules
Mingxia Sun, Chongdong Liu, Na Zhao, Kaikai Meng, Zhenyu Zhang
The present study investigated the predictive value of the platelet aggregation rate in postpartum deep venous thrombosis and its possible mechanism. From January 2014 to January 2016, 23 patients with postpartum deep vein thrombosis of lower extremity treated in the Department of Obstetrics of Beijing Chaoyang Hospital were as assigned as the observation group. At the same time, 25 cases with normal recovery were assigned as the control group. Blood samples were collected from all the subjects. The platelet aggregation rate was measured using a platelet aggregation apparatus...
June 2018: Experimental and Therapeutic Medicine
Angélica Salas-Perdomo, Francesc Miró-Mur, Xabier Urra, Carles Justicia, Mattia Gallizioli, Yashu Zhao, Vanessa H Brait, Carlos Laredo, Raúl Tudela, Andrés Hidalgo, Ángel Chamorro, Anna M Planas
OBJECTIVE: Hemorrhagic transformation is a serious complication of ischemic stroke after recanalization therapies. This study aims to identify mechanisms underlying hemorrhagic transformation after cerebral ischemia/reperfusion. APPROACH AND RESULTS: We used wild-type mice and Selplg-/- and Fut7-/- mice defective in P-selectin binding and lymphopenic Rag2-/- mice. We induced 30-minute or 45-minute ischemia by intraluminal occlusion of the middle cerebral artery and assessed hemorrhagic transformation at 48 hours with a hemorrhage grading score, histological means, brain hemoglobin content, or magnetic resonance imaging...
June 14, 2018: Arteriosclerosis, Thrombosis, and Vascular Biology
Stefan Brunner, Konstantinos Rizas, Wolfgang Hamm, Michael Mehr, Korbinian Lackermair
It is known that physical exercise may increase platelet activity. However, the effect of exercise on platelet reactivity in patients on dual antiplatelet therapy has not been investigated yet. In our study, 21 patients with coronary artery disease on dual antiplatelet therapy and 10 controls were enrolled. We performed an exercise test using a cycle ergometer and determined the adenosine diphosphate-induced platelet reactivity before and immediately after exercise testing. Additionally, we analysed maximal exercise capacity and an electrocardiogram...
June 14, 2018: International Journal of Sports Medicine
Sascha Marx, Maximilian Splittstöhser, Frederik Kinnen, Eileen Moritz, Christy Joseph, Sebastian Paul, Heiko Paland, Carolin Seifert, Madlen Marx, Andreas Böhm, Edzard Schwedhelm, Kerstin Holzer, Stephan Singer, Christoph A Ritter, Sandra Bien-Möller, Henry W S Schroeder, Bernhard H Rauch
Patients with glioblastoma multiforme (GBM) suffer from an increased incidence of vascular thrombotic events. However, key influencing factors of the primary hemostasis have not been characterized in GBM patients to date. Thus, the present study determines the activation level of circulating platelets in GBM patients, in-vitro reactivity to agonist-induced platelet stimulation and the formation of circulating platelet-leucocyte conjugates as well as the plasma levels of the proinflammatory lipid mediator sphingosine-1-phosphate (S1P)...
May 25, 2018: Oncotarget
Anna Holthenrich, Volker Gerke
Endothelial cells serve as gatekeepers of vascular hemostasis and local inflammatory reactions. They can rapidly respond to changes in the environment, caused, for example, by blood vessel injury, tissue damage or infection, by secreting in a strictly regulated manner factors regulating these processes. These factors include adhesion receptors for circulating leukocytes and platelets, P-selectin and von-Willebrand factor (VWF) that are stored in specialized secretory granules of endothelial cells, the Weibel-Palade bodies (WPB)...
June 13, 2018: International Journal of Molecular Sciences
Fariborz Soroush, Yuan Tang, Hasan M Zaidi, Joel B Sheffield, Laurie E Kilpatrick, Mohammad F Kiani
In the event of a radiologic catastrophe, endothelial cell and neutrophil dysfunction play important roles in tissue injury. Clinically available therapeutics for radiation-induced vascular injury are largely supportive. PKCδ was identified as a critical regulator of the inflammatory response, and its inhibition was shown to protect critical organs during sepsis. We used a novel biomimetic microfluidic assay (bMFA) to interrogate the role of PKCδ in radiation-induced neutrophil-endothelial cell interaction and endothelial cell function...
June 13, 2018: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
Jelena Kornej, Petra Büttner, Philipp Sommer, Nikolaos Dagres, Borislav Dinov, Katja Schumacher, Andreas Bollmann, Gerhard Hindricks
Aims: Low voltage areas (LVAs) represent advanced remodelling processes in left atrium in patients with atrial fibrillation (AF) and are associated with higher rates of arrhythmia recurrences. However, the prediction of LVA based on clinical parameters is understudied. Recently, we introduced APPLE score to predict rhythm outcomes after catheter ablation. The aim of this study was to analyse (i) LVA prediction using APPLE score and (ii) differences in biomarker profiles according to APPLE score in AF patients...
June 8, 2018: Europace: European Pacing, Arrhythmias, and Cardiac Electrophysiology
Tracy Stokol, Priscila B S Serpa, Marjory B Brooks, Thomas Divers, Sally Ness
Equine herpesvirus type 1 (EHV-1) is a major cause of infectious respiratory disease, abortion and neurologic disease. Thrombosis in placental and spinal vessels and subsequent ischemic injury in EHV-1-infected horses manifests clinically as abortion and myeloencephalopathy. We have previously shown that addition of heparin anticoagulants to equine platelet-rich plasma (PRP) can abolish ex vivo EHV-1-induced platelet activation. The goal of this study was to test whether platelets isolated from horses treated with unfractionated heparin (UFH) or low-molecular-weight heparin (LMWH) were resistant to ex vivo EHV-1-induced activation...
2018: Frontiers in Veterinary Science
Haibin Tong, Siya Wu, Kangxing Song, Jian Liu, Xindan Song, Xu Zhang, Luqi Huang, Mingjiang Wu
P-selectin is a promising therapeutic target for acute inflammation-related diseases, and interest has been growing in the search for high-affinity glycoconjugate ligands that can target the initial P-selectin-mediated recruitment of neutrophils to the site of inflammation. In our previous study, we isolated a water-soluble polysaccharide (BCPS) from Bupleurum chinense and showed that it exhibits anti-inflammatory effect by antagonizing P-selectin-mediated adhesion of HL-60 cells to CHO-P cells. In this study, we prepared a P-selectin-based affinity chromatography medium and used it to purify the P-selectin-binding moiety of BCPS...
September 15, 2018: Carbohydrate Polymers
Muhammad Irfan, Da Hye Jeong, Hyuk-Woo Kwon, Jung-Hae Shin, Sang-Joon Park, Dongmi Kwak, Tae-Hwan Kim, Dong-Ha Lee, Hwa-Jin Park, Man Hee Rhee
BACKGROUND & PURPOSE: Ginseng (Panax ginseng C.A. Mayer) contains saponin fractions called ginsenosides, which are thought to be the main components responsible for its various pharmacological activities. Ginsenosides have cardioprotective and antiplatelet effects. In the present study, we evaluated the effects of ginsenoside Rp3 (G-Rp3) on platelet function. METHODS: The in vitro effects of G-Rp3 were evaluated on agonist-induced human and rat platelet aggregation, while [Ca2+ ]i mobilization, granule secretion, integrin αIIb β3 activation, and clot retraction were assessed in rat platelets...
June 8, 2018: Vascular Pharmacology
Constance C F M J Baaten, Floor C J I Moenen, Yvonne M C Henskens, Frauke Swieringa, Rick J H Wetzels, René van Oerle, Harry F G Heijnen, Hugo Ten Cate, Graham P Holloway, Erik A M Beckers, Johan W M Heemskerk, Paola E J van der Meijden
Severe thrombocytopenia (≤50x109 platelets/L) due to hematological malignancy and intensive chemotherapy is associated with an increased risk of clinically significant bleeding. Since the bleeding risk is not linked to the platelet count only, other hemostatic factors must be involved. In 77 patients with acute leukemia, multiple myeloma or malignant lymphoma, who experienced chemotherapy-induced thrombocytopenia, we studied platelet function. Platelets from all patients - independent of disease or treatment type - were to a variable extent compromised in Ca2+ flux, integrin αIIbβ3 activation and P-selectin expression, when stimulated with a panel of agonists...
June 7, 2018: Haematologica
Marco Aurelio L Saffi, Eneida R Rabelo-Silva, Carisi A Polanczyk, Mariana V Furtado, Marlon M Montenegro, Ingrid W J Ribeiro, Cassio Kampits, Cassiano K Rösing, Alex N Haas
OBJECTIVE: To assess the effects of periodontal treatment on endothelial function in patients with coronary artery disease. MATERIALS AND METHODS: A randomized controlled trial was conducted with 69 patients with stable coronary disease and severe periodontitis. The test group received non-surgical periodontal therapy consisting of personalized oral hygiene instructions, subgingival scaling and root planing per quadrant, whereas the control group received equal treatment after the study period...
June 6, 2018: Oral Diseases
Knut A Mosevoll, Silje Johansen, Øystein Wendelbo, Ina Nepstad, Øystein Bruserud, Håkon Reikvam
The inflammatory response is a well-established part of, and a prerequisite for, venous thrombosis. To better understand the pathophysiology of venous thrombosis and to identify improved diagnostic biomarkers, further studies of the relationship between inflammation and coagulation are needed. We review previous studies concerning inflammatory biomarkers in venous thromboembolism, in particular cytokines, soluble adhesion molecules and matrix metalloproteases as predisposing, diagnostic and prognostic factors in venous thrombosis...
2018: Frontiers in Medicine
Lei Pi, Di Che, Haifeng Long, Zhenzhen Fang, Jiawen Li, Shuyi Lin, Yunfeng Liu, Meiai Li, Lijuan Bao, Wenli Li, Yuan Zhang, Qiulian Deng, Techang Liu, Li Zhang, Xiaoqiong Gu
Introduction: Kawasaki disease is a kind of systemic vasculitis that mainly damages moderate and small-sized blood vessels, and is a leading cause of coronary artery lesions (CAL). Antiplatelet therapy is a routine component of Kawasaki disease treatment strategies. So it is important to evaluate the antiplatelet effect of aspirin because of the individual biological variability of antiplatelet effect of aspirin. The immature platelet fraction (IPF) has attracted particular attention as it may influence the antiplatelet effect of aspirin...
2018: Drug Design, Development and Therapy
Bernard M F M Duvivier, Johanne E Bolijn, Annemarie Koster, Casper G Schalkwijk, Hans H C M Savelberg, Nicolaas C Schaper
Recent studies suggest that substituting sitting with light physical activity has beneficial metabolic effects, but it is unclear if this is associated with parallel changes in endothelial function. Data from three randomized cross-over studies were analyzed, in which 61 subjects (with normal weight, overweight and type 2 diabetes) followed different activity regimens (Sit, SitLess and/or Exercise) of four days each. Subjects were instructed to sit 14 h/day ('Sit'), to substitute 1 h/day of sitting with moderate-to-vigorous cycling ('Exercise') or to substitute 5-6 h/day sitting with light-intensity walking and standing ('SitLess')...
June 5, 2018: Scientific Reports
Harpreet S Bajaj, Chang Ye, Anthony J Hanley, Mathew Sermer, Bernard Zinman, Ravi Retnakaran
OBJECTIVE: Women with gestational diabetes mellitus and milder gestational impaired glucose intolerance have elevated future risks of type 2 diabetes and cardiovascular disease. However, it is unclear whether they show postpartum evidence of vascular injury/dysfunction, an early event in the natural history of cardiovascular disease. METHODS: In total, 337 women underwent a glucose challenge test and oral glucose tolerance test in pregnancy, yielding four gestational glucose tolerance groups: gestational diabetes mellitus, gestational impaired glucose intolerance, abnormal glucose challenge test with normal glucose tolerance on the oral glucose tolerance test and normal glucose challenge test with normal glucose tolerance...
June 1, 2018: Diabetes & Vascular Disease Research
Susanne Claudia Gruber, Matthias Karl Freynhofer, Martin Willheim, Thomas Werner Weiss, Florian Egger, Wolfgang Hübl, Kurt Huber
Long-term evidence shows an increased risk of cardiovascular events in the morning hours and recent studies in aspirin-treated patients have shown increased platelet reactivity at the end of the dosing interval. Similar pharmacodynamic analyses of adenosine-diphosphate (ADP) receptor inhibitors are scarce. We therefore investigated changes in clopidogrel-dependent platelet function and activation over 24 h and whether enhanced platelet turnover might explain diurnal variability of platelet function and activation...
June 5, 2018: Platelets
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