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https://www.readbyqxmd.com/read/28971586/dietary-n-glycans-from-bovine-lactoferrin-and-tlr-modulation
#1
Susana Figueroa-Lozano, Rivca L Valk-Weeber, Sander S van Leeuwen, Lubbert Dijkhuizen, Paul de Vos
SCOPE: Bovine lactoferrin (bLF) is an ingredient of food supplements and infant formulas given its antimicrobial and antiviral properties. We modified bLF enzymatically to alter its N-glycosylation and to isolate the glycan chains. The aims of this study were 1) to evaluate whether such derivates induce responses via Pattern Recognition Receptors (PRRs) namely Toll-like receptors (TLRs) and 2) to relate those responses to their different glycosylation profiles. METHODS AND RESULTS: The unmodified and modified bLF fractions were incubated with reporter cell lines expressing PRRs...
October 3, 2017: Molecular Nutrition & Food Research
https://www.readbyqxmd.com/read/28963751/tiomax-a-spanish-multicenter-registry-of-the-real-world-use-of-the-titanium-optimax-%C3%A2-biostent-tiomax-registro-espa%C3%A3-ol-multic%C3%A3-ntrico-del-biostent-de-titanio-optimax-%C3%A2-en-la-vida-real
#2
José R López-Mínguez, Juan M Nogales-Asensio, Sebastián Romani, Fernando Rivero-Crespo, Victor M Aragón-Extremera, Jesús M Jiménez-Mazuecos, Fernando Carrasco, Juan Francisco Oteo-Domínguez, Francisco Bosa-Ojeda, Joan A Gómez-Hospital
OBJECTIVES: To compare the safety and efficacy of the new cobalt-chromium bioactive stent Titan Optimax(®) (Hexacath, France) with its predecessor, Titan-2(®) . BACKGROUND: The TIOMAX registry includes 784 patients who underwent percutaneous coronary intervention with these stents in 21 Spanish hospitals. METHODS: Analysis of all patients in the registry without exclusion criteria, candidates for revascularization (March-2013/July-2014)...
September 30, 2017: Catheterization and Cardiovascular Interventions
https://www.readbyqxmd.com/read/28963016/delivery-of-the-tlr-ligand-poly-i-c-to-liver-cells-in-vitro-and-in-vivo-by-calcium-phosphate-nanoparticles-leads-to-a-pronounced-immunostimulation
#3
Viktoriya Sokolova, Zou Shi, Shunmei Huang, Yanqin Du, Mathis Kopp, Annika Frede, Torben Knuschke, Jan Buer, Dongliang Yang, Jun Wu, Astrid Maria Westendorf, Matthias Epple
The selective activation of the immune system is a concurrent problem in the treatment of persistent diseases like viral infections (e.g. hepatitis). For the delivery of the toll-like receptor ligand poly(I:C), an immunostimulatory action was discovered earlier by hydrodynamic injection. However, this technique is not clinically transferable to human patients. A modular system where the immunoactive toll-like-receptor ligand 3 (TLR-3) poly(I:C) was incorporated into calcium phosphate nanoparticles was developed...
September 27, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28924346/co-delivery-of-polyinosinic-polycytidylic-acid-and-flagellin-by-poly-lactic-co-glycolic-acid-mps-synergistically-enhances-immune-response-elicited-by-intranasally-delivered-hepatitis-b-surface-antigen
#4
Xiaojing Dai, Jintian He, Ruxia Zhang, Guanghao Wu, Fangfang Xiong, Baohua Zhao
The aim of the present work was to investigate the synergistic effect between toll-like receptor (TLR) 3 ligand polyinosinic:polycytidylic acid (pI:C) and TLR5 ligand flagellin (FLN) on immune responses induced by nasally delivered hepatitis B virus surface antigen (HBsAg). Mannan and chitosan oligosaccharide-modified, pH-responsive poly(lactic-co-glycolic acid) (MC-PLGA) microparticles (MPs) containing HBsAg, FLN, pI:C or both ligands were prepared with a double-emulsion method. In vitro uptake experiments show that cellular uptake of MC-PLGA MPs by macrophages was through energy-dependent, receptor-mediated endocytosis mechanism...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/28921745/transcription-factor-ccaat-enhancer-binding-protein-%C3%AE-upregulates-microrna-let-7f-1-in-human-endocervical-cells
#5
Kanchana Ayyar, Kudumula Venkata Rami Reddy
PROBLEM: In endocervical epithelial cells (End1/E6E7), miRNA let-7f plays an important role in the control of innate immunity. The underlying molecular mechanism for let-7f regulation in these cells remains largely unclear. METHODS OF STUDY: let-7f was knocked down in End1/E6E7 cells using siRNA, and differential gene expression was analyzed by microarray. Differentially expressed genes were validated by qPCR and Western blot. Expression of let-7f was studied by qPCR after inhibition of C/EBPβ with betulinic acid (BA) and pCMVβ plasmid and after overexpression of C/EBPβ with pCMVβ+ plasmid...
September 16, 2017: American Journal of Reproductive Immunology: AJRI
https://www.readbyqxmd.com/read/28900156/dual-roles-of-two-isoforms-of-autophagy-related-gene-atg10-in-hcv-subgenomic-replicon-mediated-autophagy-flux-and-innate-immunity
#6
Qiong Zhao, Zhan-Ying Hu, Jing-Pu Zhang, Jian-Dong Jiang, Yuan-Yuan Ma, Jian-Rui Li, Zong-Gen Peng, Jin-Hua Chen
Autophagy and immune response are two defense systems that human-body uses against viral infection. Previous studies documented that some viral mechanisms circumvented host immunity mechanisms and hijacked autophagy for its replication and survival. Here, we focus on interactions between autophagy mechanism and innate-immune-response in HCV-subgenomic replicon cells to find a mechanism linking the two pathways. We report distinct effects of two autophagy-related protein ATG10s on HCV-subgenomic replication...
September 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28891416/role-of-innate-immunity-in-preeclampsia-a-systematic-review
#7
Ana P Bouças, Bianca M de Souza, Andrea C Bauer, Daisy Crispim
Innate immune system dysfunction has been known to be a key player in preeclampsia (PE). Activation of the maternal innate immunity may be triggered by invading microorganisms or endogenous ligands, which are detected by different pattern recognition receptors (PRRs). Although some studies have linked PRR activation to PE, it is still unclear if dysregulated PRR expression is associated with the development of this complication. Therefore, we conducted a systematic review of the literature, searching articles that evaluated associations of PRRs with PE...
October 2017: Reproductive Sciences
https://www.readbyqxmd.com/read/28878771/coordinated-role-of-toll-like-receptor-3-and-retinoic-acid-inducible-gene-i-in-the-innate-response-of-bovine-endometrial-cells-to-virus
#8
Luisa C Carneiro, Carmen Bedford, Sarah Jacca, Alfonso Rosamilia, Vera F de Lima, Gaetano Donofrio, I Martin Sheldon, James G Cronin
Bovine herpesvirus-4 (BoHV-4) and bovine viral diarrhea virus (BVDV) infect the uterus of cattle, often resulting in reduced fertility, or abortion of the fetus, respectively. Here, exposure of primary bovine endometrial cells to BoHV-4 or BVDV modulated the production of inflammatory mediators. Viral pathogen-associated molecular patterns (PAMPs) are detected via pattern-recognition receptors (PRRs). However, the relative contribution of specific PRRs to innate immunity, during viral infection of the uterus, is unclear...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28878760/respiratory-commensal-bacteria-corynebacterium-pseudodiphtheriticum-improves-resistance-of-infant-mice-to-respiratory-syncytial-virus-and-streptococcus-pneumoniae-superinfection
#9
Paulraj Kanmani, Patricia Clua, Maria G Vizoso-Pinto, Cecilia Rodriguez, Susana Alvarez, Vyacheslav Melnikov, Hideki Takahashi, Haruki Kitazawa, Julio Villena
Corynebacterium pseudodiphtheriticum is a Gram-positive bacterium found as a member of the normal microbiota of the upper respiratory tract. It was suggested that C. pseudodiphtheriticum may be potentially used as a next-generation probiotic for nasal application, although no deep studies were performed in this regard. We hypothesized that human isolate C. pseudodiphtheriticum strain 090104 is able to modulate the respiratory innate immune response and beneficially influence the resistance to viral and bacterial infections...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28872665/expression-of-human-cathelicidin-peptide-ll-37-in-inflammatory-bowel-disease
#10
Shoko Kusaka, Atsushi Nishida, Kenichiro Takahashi, Shigeki Bamba, Hiroyuki Yasui, Masahiro Kawahara, Osamu Inatomi, Mitsushige Sugimoto, Akira Andoh
Cathelicidin peptide LL-37 plays an important role in the early host response against invading pathogens via its broad-spectrum antimicrobial activity. In this study, we investigated LL-37 expression in the inflamed mucosa of IBD patients. Furthermore, the regulatory mechanism of LL-37 induction was investigated in human colonic subepithelial myofibroblasts (SEMFs). LL-37 mRNA expression and protein secretion were analyzed using real-time polymerase chain reaction and enzyme-linked immunosorbent assay, respectively...
September 5, 2017: Clinical and Experimental Immunology
https://www.readbyqxmd.com/read/28835708/exploring-dengue-genome-to-construct-a-multi-epitope-based-subunit-vaccine-by-utilizing-immunoinformatics-approach-to-battle-against-dengue-infection
#11
Mudassar Ali, Rajan Kumar Pandey, Nazia Khatoon, Aruna Narula, Amit Mishra, Vijay Kumar Prajapati
Dengue is considered as a major health issue which causes a number of deaths worldwide each year; tropical countries are majorly affected by dengue outbreaks. It is considered as life threatening issue because, since many decades not a single effective approach for treatment and prevention of dengue has been developed. Therefore, to find new preventive measure, we used immunoinformatics approaches to develop a multi-epitope based subunit vaccine for dengue which can generate various immune responses inside the host...
August 23, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28831740/expression-of-mir-155-associated-with-toll-like-receptors-3-7-and-9-transcription-in-the-olfactory-bulbs-of-cattle-naturally-infected-with-bhv5
#12
Bruna R S M Oliveira, Flavia V Vieira, Dielson de S Vieira, Sergio E L da Silva, Roberto Gameiro, Eduardo F Flores, Tereza C Cardoso
Bovine herpesvirus 5 (BHV5) infection of young cattle is frequently associated with fatal neurological disease and, as such, represents an attractive model for studying the pathogenesis of viral-induced meningoencephalitis. Following replication in the nasal mucosa, BHV5 invades the central nervous system (CNS) mainly through the olfactory pathway. The innate immune response triggered by the host face to virus replication through the olfactory route is poorly understood. Recently, an upregulation of conserved pathogen-associated molecular pattern, as Toll-like receptors (TLRs), has been demonstrated in the CNS of BHV5 experimentally infected cows...
August 22, 2017: Journal of Neurovirology
https://www.readbyqxmd.com/read/28807557/the-role-of-toll-like-receptor-3-in-chronic-contact-hypersensitivity-induced-by-repeated-elicitation
#13
Risa Yasuike, Risa Tamagawa-Mineoka, Mayumi Ueta, Naomi Nakamura, Shigeru Kinoshita, Norito Katoh
BACKGROUND: Accumulating evidence suggests that Toll-like receptor (TLR)-3 signaling is involved in non-infectious immune and inflammatory reactions as well as in viral infections. The skin of patients with atopic dermatitis (AD) is often infected with virus and bacteria, leading to the aggravation of atopic symptoms. These findings suggest TLR3 signaling may be involved in the pathogenesis of AD, but the exact role of TLR3 in AD remains to be defined. OBJECTIVE: The purpose of this study was to investigate the role of TLR3 in chronic contact hypersensitivity reactions induced by repeated elicitation, resembling the features of AD...
August 4, 2017: Journal of Dermatological Science
https://www.readbyqxmd.com/read/28800975/exopolysaccharides-from-lactobacillus-delbrueckii-oll1073r-1-modulate-innate-antiviral-immune-response-in-porcine-intestinal-epithelial-cells
#14
Paulraj Kanmani, Leonardo Albarracin, Hisakazu Kobayashi, Hikaru Iida, Ryoya Komatsu, A K M Humayun Kober, Wakako Ikeda-Ohtsubo, Yoshihito Suda, Hisashi Aso, Seiya Makino, Hiroshi Kano, Tadao Saito, Julio Villena, Haruki Kitazawa
Previous studies demonstrated that the extracellular polysaccharides (EPSs) produced by Lactobacillus delbrueckii OLL1073R-1 (LDR-1) improve antiviral immunity, especially in the systemic and respiratory compartments. However, it was not studied before whether those EPSs are able to beneficially modulate intestinal antiviral immunity. In addition, LDR-1-host interaction has been evaluated mainly with immune cells while its interaction with intestinal epithelial cells (IECs) was not addressed before. In this work, we investigated the capacity of EPSs from LDR-1 to modulate the response of porcine IECs (PIE cells) to the stimulation with the Toll-like receptor (TLR)-3 agonist poly(I:C) and the role of TLR2, TLR4, and TLR negative regulators in the immunoregulatory effect...
August 8, 2017: Molecular Immunology
https://www.readbyqxmd.com/read/28726298/faah-but-not-magl-inhibition-modulates-acute-tlr3-induced-neuroimmune-signaling-in-the-rat-independent-of-sex
#15
Lisa E Flannery, Rebecca J Henry, Daniel M Kerr, David P Finn, Michelle Roche
Toll-like receptor (TLR)3 is a key component of the innate immune response to viral infection. The present study firstly examined whether sex differences exist in TLR3-induced inflammatory, endocrine, and sickness responses. The data revealed that TLR3-induced expression of interferon- or NFkB-inducible genes (IFN-α/β, IP-10, or TNF-α), either peripherally (spleen) or centrally (hypothalamus), did not differ between male and female rats, with the exception of TLR3-induced IFN-α expression in the spleen of female, but not male, rats 8 hr post TLR3 activation...
July 20, 2017: Journal of Neuroscience Research
https://www.readbyqxmd.com/read/28687988/initial-and-late-efficacy-of-everolimus-eluting-stents-for-small-and-non-small-coronary-lesions-from-evaluating-delayed-late-loss-study
#16
Naoto Tama, Hiroyasu Uzui, Yuki Horita, Masanobu Namura, Hiroshi Tada
The aim of the present study was to evaluate the long-term outcomes at 2 years in patients in whom everolimus-eluting stents (EESs) were implanted in small and non-small vessels. A small vessel is an important risk factor for restenosis with BMSs, even in the first generation DESs. The 690 patients with 690 lesions implanted with an EES were enrolled and divided into two groups by vessel reference diameter (RD): >2.5 mm for non-small vessels (Non-S-group) and ≤2.5 mm for small vessels (S-group). Two years later, the 365 patients with no restenosis at 8 months who underwent angiography were enrolled into the late catch-up study...
July 7, 2017: Heart and Vessels
https://www.readbyqxmd.com/read/28670908/effects-of-lactobacillus-rhamnosus-gg-on-the-maturation-and-differentiation-of-dendritic-cells-in-rotavirus-infected-mice
#17
Y Jiang, L Ye, Y Cui, G Yang, W Yang, J Wang, J Hu, W Gu, C Shi, H Huang, C Wang
Rotavirus-related diarrhoea is considered one of the most important diseases in field animal production. In addition to the classic vaccine strategy, a number of studies have utilised probiotics, such as Lactobacillus rhamnosus GG (LGG), to prevent rotavirus-induced diarrhoea. Although it has been partially revealed that Toll-like receptors (TLRs) are involved in the LGG-mediated protection against rotavirus infection, the details of the underlying immunologic mechanisms remain largely unknown. In this study, three-to-four-week-old female BALB/c mice were divided into three groups and orally administered phosphate buffered saline (PBS), PBS plus rotavirus or LGG plus rotavirus, respectively...
August 24, 2017: Beneficial Microbes
https://www.readbyqxmd.com/read/28624224/optimizing-the-dna-donor-template-for-homology-directed-repair-of-double-strand-breaks
#18
Fei Song, Knut Stieger
The CRISPR-Cas (clustered regularly interspaced short palindromic repeats-associated proteins) technology enables rapid and precise genome editing at any desired genomic position in almost all cells and organisms. In this study, we analyzed the impact of different repair templates on the frequency of homology-directed repair (HDR) and non-homologous end joining (NHEJ). We used a stable HEK293 cell line expressing the traffic light reporter (TLR-3) system to quantify HDR and NHEJ events following transfection with Cas9, eight different guide RNAs, and a 1,000 bp donor template generated either as circular plasmid, as linearized plasmid with long 3' or 5' backbone overhang, or as PCR product...
June 16, 2017: Molecular Therapy. Nucleic Acids
https://www.readbyqxmd.com/read/28620261/single-nucleotide-polymorphism-based-genetic-diversity-analysis-of-the-kilakarsal-and-vembur-sheep-breeds
#19
Rathinasamy Selvam, Nagarajan Murali, A Kannan Thiruvenkadan, Ramesh Saravanakumar, Gurusamy Ponnudurai, Thilak Pon Jawahar
AIM: The present study was thus undertaken to analyze the genetic diversity of Kilakarsal and Vembur sheep breeds using single-nucleotide polymorphism (SNP) markers within Toll-like receptor (TLR) 3, 5, 6, 9, and 10 genes. MATERIALS AND METHODS: Competitive allele-specific polymerase chain reaction (PCR)-based end-point genotyping was performed using real-time PCR to type the SNPs. Allele discrimination module implemented in real-time PCR was utilized to call the genotypes based on fluorescence intensity recorded for each of the two alleles...
May 2017: Veterinary World
https://www.readbyqxmd.com/read/28613415/composition-and-functionality-of-the-intrahepatic-innate-lymphoid-cell-compartment-in-human-nonfibrotic-and-fibrotic-livers
#20
Marianne Forkel, Lena Berglin, Eliisa Kekäläinen, Adrian Carlsson, Emma Svedin, Jakob Michaëlsson, Maho Nagasawa, Jonas S Erjefält, Michiko Mori, Malin Flodström-Tullberg, Annika Bergquist, Hans-Gustaf Ljunggren, Magnus Westgren, Ulrik Lindforss, Danielle Friberg, Carl Jorns, Ewa Ellis, Niklas K Björkström, Jenny Mjösberg
Human innate lymphoid cells have been described to exist in different organs, with functional deregulation of these cells contributing to several disease states. Here, we performed the first detailed characterization of the phenotype, tissue-residency properties, and functionality of ILC1s, ILC2s, and ILC3s in the human adult and fetal liver. In addition, we investigated changes in the ILC compartment in liver fibrosis. A unique composition of tissue-resident ILCs was observed in nonfibrotic livers as compared with that in mucosal tissues, with NKp44(-) ILC3s accounting for the majority of total intrahepatic ILCs...
June 14, 2017: European Journal of Immunology
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