keyword
MENU ▼
Read by QxMD icon Read
search

Bacteria secretion system

keyword
https://www.readbyqxmd.com/read/28636560/lipocalin-2-regulates-intestine-bacterial-survival-by-interplaying-with-siderophore-in-a-weaned-piglet-model-of-escherichia-coli-infection
#1
Bing-Xiu Guo, Qian-Qian Wang, Jia-Hui Li, Zhen-Shun Gan, Xiao-Feng Zhang, Yi-Zhen Wang, Hua-Hua Du
Iron is an essential nutrient that facilitates cell proliferation and growth, which plays a pivotal role in modulating the battle for survival between mammalian hosts and their pathogens. Pathogenic bacteria secrete siderophores to acquire iron from the host. However, lipocalin 2 (Lcn2), a siderophore-binding antimicrobial protein, binds to siderophores to prevent bacterial uptake of iron, which is critical for the control of systemic infection with Escherichia coli (E. coli). But few studies focus on the anti-infective response of Lcn2 in the intestines by inhibiting bacterial proliferation based on microbial iron metabolism...
June 16, 2017: Oncotarget
https://www.readbyqxmd.com/read/28635589/regulation-of-escherichia-coli-pathogenesis-by-alternative-sigma-factor-n
#2
James T Riordan, Avishek Mitra
σ(N) (also σ(54)) is an alternative sigma factor subunit of the RNA polymerase complex that regulates the expression of genes from many different ontological groups. It is broadly conserved in the Eubacteria with major roles in nitrogen metabolism, membrane biogenesis, and motility. σ(N) is encoded as the first gene of a five-gene operon including rpoN (σ(N)), ptsN, hpf, rapZ, and npr that has been genetically retained among species of Escherichia, Shigella, and Salmonella. In an increasing number of bacteria, σ(N) has been implicated in the control of genes essential to pathogenic behavior, including those involved in adherence, secretion, immune subversion, biofilm formation, toxin production, and resistance to both antimicrobials and biological stressors...
June 2017: EcoSal Plus
https://www.readbyqxmd.com/read/28634572/a-helicobacter-pylori-homolog-of-eukaryotic-flotillin-is-involved-in-cholesterol-accumulation-epithelial-cell-responses-and-host-colonization
#3
Melanie L Hutton, Kimberley D'Costa, Amanda E Rossiter, Lin Wang, Lorinda Turner, David L Steer, Seth L Masters, Ben A Croker, Maria Kaparakis-Liaskos, Richard L Ferrero
The human pathogen Helicobacter pylori acquires cholesterol from membrane raft domains in eukaryotic cells, commonly known as "lipid rafts." Incorporation of this cholesterol into the H. pylori cell membrane allows the bacterium to avoid clearance by the host immune system and to resist the effects of antibiotics and antimicrobial peptides. The presence of cholesterol in H. pylori bacteria suggested that this pathogen may have cholesterol-enriched domains within its membrane. Consistent with this suggestion, we identified a hypothetical H...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28630070/regulation-of-type-iii-secretion-of-translocon-and-effector-proteins-by-the-esab-esal-esam-complex-in-edwardsiella-tarda
#4
Lu Yi Liu, Pin Nie, Hong Bing Yu, Hai Xia Xie
The type III secretion system (T3SS) plays a crucial role in the pathogenesis of many Gram-negative bacteria, including Edwardsiella tarda, an important fish pathogen. Within the E. tarda T3SS, there are three proteins (EsaB/EsaL/EsaM) that are homologous to proteins present in many other bacteria, including SpiC/SsaL/SsaM in Salmonella, SepD/SepL/CesL in EPEC and EHEC, and YscB/YopN/SycN in Yersinia EsaL was found to interact with both EsaB and EsaM within the bacterial cell, as revealed by a co-immunoprecipitation assay...
June 19, 2017: Infection and Immunity
https://www.readbyqxmd.com/read/28630067/sipa-activation-of-caspase-3-is-a-decisive-mediator-of-host-cell-survival-at-early-stages-of-salmonella-typhimurium-infection
#5
Anne McIntosh, Lynsey M Meikle, Michael Ormsby, Beth A McCormick, John M Christie, James M Brewer, Mark Roberts, Daniel M Wall
Salmonella invasion protein A (SipA) is a dual function effector protein that plays roles in both actin polymerization and caspase-3 activation in intestinal epithelial cells. To date its function in other cell types has remained largely unknown despite its expression in multiple cell types and its extracellular secretion during infection. Here we show that in macrophages SipA induces increased caspase-3 activation early in infection. This activation required a threshold level of SipA linked to multiplicity of infection and may be a limiting factor controlling bacterial numbers in infected macrophages...
June 19, 2017: Infection and Immunity
https://www.readbyqxmd.com/read/28627648/characterization-of-a-blandm%C3%A2-1%C3%A2-harboring-plasmid-from-a-salmonella%C3%A2-enterica-clinical-isolate-in-china
#6
Jinwei Huang, Shanshan Deng, Jianmin Ren, Jianfei Tu, Meiping Ye, Minggui Wang
The plasmid-mediated transmission of antibiotic resistance genes has been reported to be involved in the development of antibiotic resistance in bacteria, and poses a serious threat for the success of bacterial infection treatment and human health worldwide. The present study used a 454 GS‑FLX pyrosequencing system to determine the ~140 kb nucleotide sequence of plasmid pHS36‑NDM, which was identified in a Salmonella Stanley isolate from the stool sample of an 11‑month‑old girl at Lishui Central Hospital, China, and which contains a New Delhi metallo‑β‑lactamase‑1 (NDM‑1) carbapenem resistance gene (blaNDM‑1)...
June 8, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28625823/the-leukocyte-associated-immunoglobulin-ig-like-receptor-1-modulating-cell-apoptosis-and-inflammatory-cytokines-secretion-in-thp-1%C3%A2-cells-after-helicobacter-pylori-infection
#7
Ying Zhang, Hui Sun, Jiaojiao Li, Qianyu Rong, Xiaofei Ji, Boqing Li
OBJECTIVE: Helicobacter pylori is a Gram-negative, microaerophilic bacteria usually found in the stomach, which may evade its host's immune system and present long-term symptoms in affected individuals. This study aimed to evaluate the functional role of leukocyte-associated immunoglobulin (Ig)-like receptor-1 (LAIR-1) in the strategies and underlying molecular mechanisms by which H. pylori escapes the host's immune responses. METHODS: LAIR-1 knockdown THP-1 cells were used to detect cell apoptosis, cell proliferation, interleukin-8 (IL-8), IL-10, and activation of intracellular signaling induced by H...
June 15, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28623733/a-novel-immune-related-gene-hdd1-of-silkworm-bombyx-mori-is-involved-in-bacterial-response
#8
Kui Zhang, Guangzhao Pan, Yuzu Zhao, Xiangwei Hao, Chongyang Li, Li Shen, Rui Zhang, Jingjing Su, Hongjuan Cui
Insects have evolved an effective immune system to respond to various challenges. In this study, a novel immune-related gene, called BmHDD1, was first charactered in silkworm, Bombyx mori. BmHDD1 contained an ORF of 837bp and encoding a deduced protein of 278 amino acids. BmHDD1 was specifically expressed in hemocytes, and highly expressed at the molting and metamorphosis stages under normal physiological conditions. Our results suggested that BmHDD1 was mainly generated by hemocytes and secreted into hemolymph...
June 14, 2017: Molecular Immunology
https://www.readbyqxmd.com/read/28623321/dysbiosis-in-chronic-periodontitis-key-microbial-players-and-interactions-with-the-human-host
#9
Zhi-Luo Deng, Szymon P Szafrański, Michael Jarek, Sabin Bhuju, Irene Wagner-Döbler
Periodontitis is an extremely prevalent disease worldwide and is driven by complex dysbiotic microbiota. Here we analyzed the transcriptional activity of the periodontal pocket microbiota from all domains of life as well as the human host in health and chronic periodontitis. Bacteria showed strong enrichment of 18 KEGG functional modules in chronic periodontitis, including bacterial chemotaxis, flagellar assembly, type III secretion system, type III CRISPR-Cas system, and two component system proteins. Upregulation of these functions was driven by the red-complex pathogens and candidate pathogens, e...
June 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28622430/enterohaemorrhagic-escherichia-coli-produces-outer-membrane-vesicles-as-an-active-defense-system-against-antimicrobial-peptide-ll-37
#10
Akiko Urashima, Ayano Sanou, Hilo Yen, Toru Tobe
Antimicrobial peptides (AMPs) are important components of the innate immune system. Enterohaemorrhagic Escherichia coli (EHEC), a food-borne pathogen causing serious diarrheal diseases, must overcome attack by AMPs. Here, we show that resistance of EHEC against human cathelicidin LL-37, a primary AMP, was enhanced by butyrate, which has been shown to act as a stimulant for the expression of virulence genes. The increase of resistance depended on the activation of the ompT gene, which encodes the outer membrane protease OmpT for LL-37...
June 16, 2017: Cellular Microbiology
https://www.readbyqxmd.com/read/28622339/the-chlamydia-trachomatis-type-iii-secretion-substrates-ct142-ct143-and-ct144-are-secreted-into-the-lumen-of-the-inclusion
#11
Maria da Cunha, Sara V Pais, Joana N Bugalhão, Luís Jaime Mota
Chlamydia trachomatis is a human bacterial pathogen causing ocular and genital infections. It multiplies exclusively within an intracellular membrane-bound vacuole, the inclusion, and uses a type III secretion system to manipulate host cells by injecting them with bacterially-encoded effector proteins. In this work, we characterized the expression and subcellular localization in infected host cells of the C. trachomatis CT142, CT143, and CT144 proteins, which we previously showed to be type III secretion substrates...
2017: PloS One
https://www.readbyqxmd.com/read/28621333/francisella-requires-dynamic-type-vi-secretion-system-and-clpb-to-deliver-effectors-for-phagosomal-escape
#12
Maj Brodmann, Roland F Dreier, Petr Broz, Marek Basler
Francisella tularensis is an intracellular pathogen that causes the fatal zoonotic disease tularaemia. Critical for its pathogenesis is the ability of the phagocytosed bacteria to escape into the cell cytosol. For this, the bacteria use a non-canonical type VI secretion system (T6SS) encoded on the Francisella pathogenicity island (FPI). Here we show that in F. novicida T6SS assembly initiates at the bacterial poles both in vitro and within infected macrophages. T6SS dynamics and function depends on the general purpose ClpB unfoldase, which specifically colocalizes with contracted sheaths and is required for their disassembly...
June 16, 2017: Nature Communications
https://www.readbyqxmd.com/read/28620586/tracking-proteins-secreted-by-bacteria-what-s-in-the-toolbox
#13
REVIEW
Benoit Maffei, Olivera Francetic, Agathe Subtil
Bacteria have acquired multiple systems to expose proteins on their surface, release them in the extracellular environment or even inject them into a neighboring cell. Protein secretion has a high adaptive value and secreted proteins are implicated in many functions, which are often essential for bacterial fitness. Several secreted proteins or secretion machineries have been extensively studied as potential drug targets. It is therefore important to identify the secretion substrates, to understand how they are specifically recognized by the secretion machineries, and how transport through these machineries occurs...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28619883/spatiotemporal-monitoring-of-pseudomonas-effectors-via-type-iii-secretion-using-split-fluorescent-protein-fragments
#14
Eunsook Park, Hye-Young Lee, Jongchan Woo, Doil Choi, Savithramma P Dinesh-Kumar
Pathogenic gram-negative bacteria cause serious diseases in animals and plants. These bacterial pathogens use the type III secretion system (T3SS) to deliver effector proteins into host cells; these effectors then localize to different subcellular compartments to attenuate immune responses by altering biological processes of the host cells. The fluorescent protein (FP)-based approach to monitor effectors secreted from bacteria into the host cells is not possible because the folded FP prevents effector delivery through the T3SS...
June 14, 2017: Plant Cell
https://www.readbyqxmd.com/read/28619616/activity-independent-screening-of-secreted-proteins-using-split-gfp
#15
Andreas Knapp, Myriam Ripphahn, Kristina Volkenborn, Pia Skoczinski, Karl-Erich Jaeger
The large-scale industrial production of proteins requires efficient secretion, as provided, for instance, by the Sec system of Gram-positive bacteria. Protein engineering approaches to optimize secretion often require the screening of large libraries, e.g. comprising a target protein fused to many different signal peptides. Respective high-throughput screening methods are usually based on photometric or fluorimetric assays enabling fast and simple determination of enzymatic activities. Here, we report on an alternative method for quantification of secreted proteins based on the split GFP assay...
June 12, 2017: Journal of Biotechnology
https://www.readbyqxmd.com/read/28618206/grazing-of-leaf-associated-cercomonads-protists-rhizaria-cercozoa-structures-bacterial-community-composition-and-function
#16
Sebastian Flues, David Bass, Michael Bonkowski
Preferential food selection in protists is well documented, but we still lack basic understanding on how protist predation modifies the taxonomic and functional composition of bacterial communities. We conducted feeding trials using leaf-associated cercomonad Cercozoa by incubating them on a standardized, diverse bacterial community washed from plant leaves. We used a shotgun metagenomics approach to investigate the taxonomic and functional changes of the bacterial community after five days protist predation upon bacteria...
June 15, 2017: Environmental Microbiology
https://www.readbyqxmd.com/read/28611954/super-resolution-imaging-of-protein-secretion-systems-and-the-cell-surface-of-gram-negative-bacteria
#17
REVIEW
Sachith D Gunasinghe, Chaille T Webb, Kirstin D Elgass, Iain D Hay, Trevor Lithgow
Gram-negative bacteria have a highly evolved cell wall with two membranes composed of complex arrays of integral and peripheral proteins, as well as phospholipids and glycolipids. In order to sense changes in, respond to, and exploit their environmental niches, bacteria rely on structures assembled into or onto the outer membrane. Protein secretion across the cell wall is a key process in virulence and other fundamental aspects of bacterial cell biology. The final stage of protein secretion in Gram-negative bacteria, translocation across the outer membrane, is energetically challenging so sophisticated nanomachines have evolved to meet this challenge...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28611405/local-immunization-impacts-the-response-of-dairy-cows-to-escherichia-coli-mastitis
#18
Vincent Herry, Christophe Gitton, Guillaume Tabouret, Maryline Répérant, Laurine Forge, Christian Tasca, Florence B Gilbert, Edouard Guitton, Céline Barc, Christophe Staub, David G E Smith, Pierre Germon, Gilles Foucras, Pascal Rainard
Current vaccines to Escherichia coli mastitis have shown some albeit limited efficacy. Their mode of action has not been documented, and immune responses protecting the mammary gland against E. coli are not completely understood. To improve our knowledge of mammary gland immune protection, cows immunized either intramuscularly or intramammarily with the E. coli P4 were submitted to a homologous mastitis challenge. A third group of mock-immunized cows serve as challenge controls. Local immunization modified favorably the course of infection, by improving bacterial clearance while limiting inflammation...
June 13, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28609638/hyperbiofilm-phenotype-of-pseudomonas-aeruginosa-defective-for-the-plcb-and-plcn-secreted-phospholipases
#19
Shawn Lewenza, Laetitia Mazenod, Shirin Afroj, Erik van Tilburg Bernardes
Biofilms are dense communities of bacteria enmeshed in a protective extracellular matrix composed mainly of exopolysaccharides (EPS), extracellular DNA (eDNA), proteins and outer membrane vesicles (OMV). Given the role of biofilms in antibiotic tolerant and chronic infections, novel strategies are needed to block, disperse or degrade biofilms. Enzymes that degrade the biofilm matrix are a promising new therapy. We screened mutants in many of the enzymes secreted by the type II secretion system (T2SS) and determined that the T2SS, and specifically phospholipases, play a role in biofilm formation...
June 13, 2017: Canadian Journal of Microbiology
https://www.readbyqxmd.com/read/28603700/the-type-ix-secretion-system-t9ss-highlights-and-recent-insights-into-its-structure-and-function
#20
REVIEW
Anna M Lasica, Miroslaw Ksiazek, Mariusz Madej, Jan Potempa
Protein secretion systems are vital for prokaryotic life, as they enable bacteria to acquire nutrients, communicate with other species, defend against biological and chemical agents, and facilitate disease through the delivery of virulence factors. In this review, we will focus on the recently discovered type IX secretion system (T9SS), a complex translocon found only in some species of the Bacteroidetes phylum. T9SS plays two roles, depending on the lifestyle of the bacteria. It provides either a means of movement (called gliding motility) for peace-loving environmental bacteria or a weapon for pathogens...
2017: Frontiers in Cellular and Infection Microbiology
keyword
keyword
286
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"