keyword
MENU ▼
Read by QxMD icon Read
search

Lactic acid stress yeast

keyword
https://www.readbyqxmd.com/read/27815194/evolutionary-engineering-reveals-divergent-paths-when-yeast-is-adapted-to-different-acidic-environments
#1
Eugene Fletcher, Amir Feizi, Markus M M Bisschops, Björn M Hallström, Sakda Khoomrung, Verena Siewers, Jens Nielsen
Tolerance of yeast to acid stress is important for many industrial processes including organic acid production. Therefore, elucidating the molecular basis of long term adaptation to acidic environments will be beneficial for engineering production strains to thrive under such harsh conditions. Previous studies using gene expression analysis have suggested that both organic and inorganic acids display similar responses during short term exposure to acidic conditions. However, biological mechanisms that will lead to long term adaptation of yeast to acidic conditions remains unknown and whether these mechanisms will be similar for tolerance to both organic and inorganic acids is yet to be explored...
November 1, 2016: Metabolic Engineering
https://www.readbyqxmd.com/read/27798688/adaptive-response-of-listeria-monocytogenes-to-heat-salinity-and-low-ph-after-habituation-on-cherry-tomatoes-and-lettuce-leaves
#2
Sofia V Poimenidou, Danai-Natalia Chatzithoma, George-John Nychas, Panagiotis N Skandamis
Pathogens found on fresh produce may encounter low temperatures, high acidity and limited nutrient availability. The aim of this study was to evaluate the effect of habituation of Listeria monocytogenes on cherry tomatoes or lettuce leaves on its subsequent response to inhibitory levels of acid, osmotic and heat stress. Habituation was performed by inoculating lettuce coupons, whole cherry tomatoes or tryptic soy broth (TSB) with a three-strains composite of L. monocytogenes, which were further incubated at 5°C for 24 hours or 5 days...
2016: PloS One
https://www.readbyqxmd.com/read/27493768/cold-stress-responses-in-the-antarctic-basidiomycetous-yeast-mrakia-blollopis
#3
Masaharu Tsuji
Microbes growing at subzero temperatures encounter numerous growth constraints. However, fungi that inhabit cold environments can grow and decompose organic compounds under subzero temperatures. Thus, understanding the cold-adaptation strategies of fungi under extreme environments is critical for elucidating polar-region ecosystems. Here, I report that two strains of the Antarctic basidiomycetous yeast Mrakia blollopis exhibited distinct growth characteristics under subzero conditions: SK-4 grew efficiently, whereas TKG1-2 did not...
July 2016: Royal Society Open Science
https://www.readbyqxmd.com/read/27470533/yeast-diversity-of-sourdoughs-and-associated-metabolic-properties-and-functionalities
#4
Luc De Vuyst, Henning Harth, Simon Van Kerrebroeck, Frédéric Leroy
Together with acidifying lactic acid bacteria, yeasts play a key role in the production process of sourdough, where they are either naturally present or added as a starter culture. Worldwide, a diversity of yeast species is encountered, with Saccharomyces cerevisiae, Candida humilis, Kazachstania exigua, Pichia kudriavzevii, Wickerhamomyces anomalus, and Torulaspora delbrueckii among the most common ones. Sourdough-adapted yeasts are able to withstand the stress conditions encountered during their growth, including nutrient starvation as well as the effects of acidic, oxidative, thermal, and osmotic stresses...
July 15, 2016: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/27450514/a-low-ph-does-not-determine-the-community-dynamics-of-spontaneously-developed-backslopped-liquid-wheat-sourdoughs-but-does-influence-their-metabolite-kinetics
#5
Simon Van Kerrebroeck, Francisca Casanova C Bastos, Henning Harth, Luc De Vuyst
This study dealt with the influence of a crucial pH value of 4.0 on the microbiota of spontaneously fermented backslopped liquid wheat sourdoughs. Two spontaneously fermented wheat sourdough fermentation experiments were carried out, one without control of the pH and one with the pH kept constant at pH4.0, both during nine backslopping steps. In each case, two additional backslopping steps were carried out, with the pH kept constant at 4.0 and with free pH, respectively. Keeping the pH constant at 4.0 changed the microbial community dynamics and metabolite kinetics of the sourdough fermentations...
July 14, 2016: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/27432472/quantitative-mass-spectrometry-based-multiplexing-compares-the-abundance-of-5000-s-cerevisiae-proteins-across-10-carbon-sources
#6
Joao A Paulo, Jeremy D O'Connell, Robert A Everley, Jonathon O'Brien, Micah A Gygi, Steven P Gygi
UNLABELLED: The budding yeast Saccharomyces cerevisiae is a model system for investigating biological processes. Cellular events are known to be dysregulated due to shifts in carbon sources. However, the comprehensive proteomic alterations thereof have not been fully investigated. Here we examined proteomic alterations in S. cerevisiae due to the adaptation of yeast from glucose to nine different carbon sources - maltose, trehalose, fructose, sucrose, glycerol, acetate, pyruvate, lactic acid, and oleate...
October 4, 2016: Journal of Proteomics
https://www.readbyqxmd.com/read/27350544/positive-effects-of-proline-addition-on-the-central-metabolism-of-wild-type-and-lactic-acid-producing-saccharomyces-cerevisiae-strains
#7
Riyanto Heru Nugroho, Katsunori Yoshikawa, Fumio Matsuda, Hiroshi Shimizu
In Saccharomyces cerevisiae, proline is a stress protectant interacting with other substrate uptake systems against oxidative stress under low pH conditions. In this study, we performed metabolomics analysis to investigate the response associated with an increase in cell growth rates and maximum densities when cells were treated with proline under normal and acid stress conditions. Metabolome data show that concentrations of components of central metabolism are increased in proline-treated S. cerevisiae. No consumption of proline was observed, suggesting that proline does not act as a nutrient but regulates metabolic state and growth of cells...
November 2016: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/27325743/engineering-of-synthetic-stress-responsive-yeast-promoters
#8
Arun S Rajkumar, Guodong Liu, David Bergenholm, Dushica Arsovska, Mette Kristensen, Jens Nielsen, Michael K Jensen, Jay D Keasling
Advances in synthetic biology and our understanding of the rules of promoter architecture have led to the development of diverse synthetic constitutive and inducible promoters in eukaryotes and prokaryotes. However, the design of promoters inducible by specific endogenous or environmental conditions is still rarely undertaken. In this study, we engineered and characterized a set of strong, synthetic promoters for budding yeast Saccharomyces cerevisiae that are inducible under acidic conditions (pH ≤ 3). Using available expression and transcription factor binding data, literature on transcriptional regulation, and known rules of promoter architecture we improved the low-pH performance of the YGP1 promoter by modifying transcription factor binding sites in its upstream activation sequence...
September 30, 2016: Nucleic Acids Research
https://www.readbyqxmd.com/read/27175476/three-week-dietary-intervention-using-apricots-pomegranate-juice-or-and-fermented-sour-sobya-and-impact-on-biomarkers-of-antioxidative-activity-oxidative-stress-and-erythrocytic-glutathione-transferase-activity-among-adults
#9
Mostafa Gouda, Amr Moustafa, Laila Hussein, Mohamed Hamza
BACKGROUND: The beneficial effects of the polyphenol (PP) rich fruits and Lactic acid bacteria fermented foods had been reported as cost-effective strategies for health promotion. Randomized controlled trial was designed to test the hypothesis that daily intake of polyphenol rich pomegranate juice (PGJ) or/ and lactic acid bacteria fermented sobya (FS) improved selected biomarkers of relevance to heath status. METHODS: The design of the human trial consisted of 35 healthy adults, who were distributed to 5 equal groups; The first group served as control and received no supplements; the second group received fresh apricot fruits (200 g); the third (PGJ) (250 g), the fourth a mixture of PGJ (150 g) and FS (140 g) and the fifth group received (FS) (170 g)...
2016: Nutrition Journal
https://www.readbyqxmd.com/read/27102264/overexpression-of-esbp6-improves-lactic-acid-resistance-and-production-in-saccharomyces-cerevisiae
#10
Minetaka Sugiyama, Shin-Pei Akase, Ryota Nakanishi, Yoshinobu Kaneko, Satoshi Harashima
Polylactic acid plastics are receiving increasing attention for the control of atmospheric CO2 emissions. Lactic acid, the building block for polylactic acid, is produced by fermentation technology from renewable carbon sources. The yeast Saccharomyces cerevisiae, harboring the lactate dehydrogenases gene (LDH), produces lactic acid at a large scale due to its strong acid resistance, to its simple nutritional requirements and to its ease of genetic engineering. Since improvement of lactic acid resistance is correlated with an increase of lactic acid production under non-neutralizing condition, we isolated a novel gene that enhances lactic acid resistance using a multi-copy yeast genomic DNA library...
October 2016: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/26887851/protein-aggregation-and-membrane-lipid-modifications-under-lactic-acid-stress-in-wild-type-and-opi1-deleted-saccharomyces-cerevisiae-strains
#11
Nadia Maria Berterame, Danilo Porro, Diletta Ami, Paola Branduardi
BACKGROUND: Lactic acid is a versatile chemical platform with many different industrial applications. Yeasts have been demonstrated as attractive alternative to natural lactic acid producers since they can grow at low pH, allowing the direct purification of the product in the desired acidic form. However, when very high concentrations of organic acids are reached, the major limitation for a viable production is the toxic effect of the product. The accumulation in the cytosol of H(+) and of the weak organic counter-anions triggers a cellular reprogramming...
2016: Microbial Cell Factories
https://www.readbyqxmd.com/read/26690924/improved-stress-resistance-and-ethanol-production-by-segmental-haploidization-of-the-diploid-genome-in-saccharomyces-cerevisiae
#12
Saeed Kaboli, Tetsuya Miyamoto, Keisuke Sunada, Yu Sasano, Minetaka Sugiyama, Satoshi Harashima
Saccharomyces cerevisiae strains from industrial and natural geographical environments are reported to show great variation in copy number of chromosomal regions. Such variation contributes to the mechanisms underlying adaptation to different environments. Here, we created and phenotypically analyzed segmentally haploidized strains, each harboring a deletion of one copy of approximately 100-300 kb of the left or right terminal region of 16 chromosomes in a diploid strain by using a PCR-mediated chromosomal deletion method...
June 2016: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/26279142/regulation-of-lactobacillus-plantarum-contamination-on-the-carbohydrate-and-energy-related-metabolisms-of-saccharomyces-cerevisiae-during-bioethanol-fermentation
#13
Shi-Jun Dong, Xiang-Hua Lin, Hao Li
During the industrial bioethanol fermentation, Saccharomyces cerevisiae cells are often stressed by bacterial contaminants, especially lactic acid bacteria. Generally, lactic acid bacteria contamination can inhibit S. cerevisiae cell growth through secreting lactic acid and competing with yeast cells for micronutrients and living space. However, whether are there still any other influences of lactic acid bacteria on yeast or not? In this study, Lactobacillus plantarum ATCC 8014 was co-cultivated with S. cerevisiae S288c to mimic the L...
November 2015: International Journal of Biochemistry & Cell Biology
https://www.readbyqxmd.com/read/26032856/inhibition-of-stress-mediated-cell-death-by-human-lactate-dehydrogenase-b-in-yeast
#14
Sara Sheibani, Natalie K Jones, Rawan Eid, Nada Gharib, Nagla T T Arab, Vladimir Titorenko, Hojatollah Vali, Paul A Young, Michael T Greenwood
We report the identification of human L- lactate dehydrogenase B (LDHB) as a novel Bax suppressor. Yeast heterologously expressing LDHB is also resistant to the lethal effects of copper indicating that it is a general suppressor of stress mediated cell death. To identify potential LDHB targets, LDHB was expressed in yeast mutants defective in apoptosis, necrosis and autophagy. The absence of functional PCD regulators including MCA1, YBH3, cyclophilin (CPR3) and VMA3, as well as the absence of the pro-survival autophagic pathway (ATG1,7) did not interfere with the LDHB mediated protection against copper indicating that LDHB functions independently of known PCD regulators or by simply blocking or stimulating a common PCD promoting or inhibitory pathway...
August 2015: FEMS Yeast Research
https://www.readbyqxmd.com/read/25947251/lactic-acid-fermentation-as-a-tool-to-enhance-the-antioxidant-properties-of-myrtus-communis-berries
#15
José Antonio Curiel, Daniela Pinto, Barbara Marzani, Pasquale Filannino, Giovanni Antonio Farris, Marco Gobbetti, Carlo Giuseppe Rizzello
BACKGROUND: Myrtle (Myrtus communis L.) is a medicinal and aromatic plant belonging to Myrtaceae family, which is largely diffused in the Mediterranean areas and mainly cultivated in Tunisia and Italy. To the best of our knowledge, no studies have already considered the use of the lactic acid fermentation to enhance the functional features of M. communis. This study aimed at using a selected lactic acid bacterium for increasing the antioxidant features of myrtle berries, with the perspective of producing a functional ingredient, dietary supplement or pharmaceutical preparation...
2015: Microbial Cell Factories
https://www.readbyqxmd.com/read/25447856/general-introduction-recombinant-protein-production-and-purification-of-insoluble-proteins
#16
REVIEW
Neus Ferrer-Miralles, Paolo Saccardo, José Luis Corchero, Zhikun Xu, Elena García-Fruitós
Proteins are synthesized in heterologous systems because of the impossibility to obtain satisfactory yields from natural sources. The production of soluble and functional recombinant proteins is among the main goals in the biotechnological field. In this context, it is important to point out that under stress conditions, protein folding machinery is saturated and this promotes protein misfolding and, consequently, protein aggregation. Thus, the selection of the optimal expression organism and the most appropriate growth conditions to minimize the formation of insoluble proteins should be done according to the protein characteristics and downstream requirements...
2015: Methods in Molecular Biology
https://www.readbyqxmd.com/read/25359316/changes-in-sam2-expression-affect-lactic-acid-tolerance-and-lactic-acid-production-in-saccharomyces-cerevisiae
#17
Laura Dato, Nadia Maria Berterame, Maria Antonietta Ricci, Paola Paganoni, Luigi Palmieri, Danilo Porro, Paola Branduardi
BACKGROUND: The great interest in the production of highly pure lactic acid enantiomers comes from the application of polylactic acid (PLA) for the production of biodegradable plastics. Yeasts can be considered as alternative cell factories to lactic acid bacteria for lactic acid production, despite not being natural producers, since they can better tolerate acidic environments. We have previously described metabolically engineered Saccharomyces cerevisiae strains producing high amounts of L-lactic acid (>60 g/L) at low pH...
2014: Microbial Cell Factories
https://www.readbyqxmd.com/read/25142694/implications-of-new-research-and-technologies-for-malolactic-fermentation-in-wine
#18
REVIEW
Krista M Sumby, Paul R Grbin, Vladimir Jiranek
The initial conversion of grape must to wine is an alcoholic fermentation (AF) largely carried out by one or more strains of yeast, typically Saccharomyces cerevisiae. After the AF, a secondary or malolactic fermentation (MLF) which is carried out by lactic acid bacteria (LAB) is often undertaken. The MLF involves the bioconversion of malic acid to lactic acid and carbon dioxide. The ability to metabolise L-malic acid is strain specific, and both individual Oenococcus oeni strains and other LAB strains vary in their ability to efficiently carry out MLF...
October 2014: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/25111117/in-silico-identification-and-characterization-of-organic-and-inorganic-chemical-stress-responding-genes-in-yeast-saccharomyces-cerevisiae
#19
Muhammad Younas Khan Barozai, Farrukh Bashir, Shafia Muzaffar, Saba Afzal, Farida Behlil, Muzaffar Khan
To study the life processes of all eukaryotes, yeast (Saccharomyces cerevisiae) is a significant model organism. It is also one of the best models to study the responses of genes at transcriptional level. In a living organism, gene expression is changed by chemical stresses. The genes that give response to chemical stresses will provide good source for the strategies in engineering and formulating mechanisms which are chemical stress resistant in the eukaryotic organisms. The data available through microarray under the chemical stresses like lithium chloride, lactic acid, weak organic acids and tomatidine were studied by using computational tools...
October 15, 2014: Gene
https://www.readbyqxmd.com/read/24682296/nuclear-localization-of-haa1-which-is-linked-to-its-phosphorylation-status-mediates-lactic-acid-tolerance-in-saccharomyces-cerevisiae
#20
Minetaka Sugiyama, Shin-Pei Akase, Ryota Nakanishi, Hitoshi Horie, Yoshinobu Kaneko, Satoshi Harashima
Improvement of the lactic acid resistance of the yeast Saccharomyces cerevisiae is important for the application of the yeast in industrial production of lactic acid from renewable resources. However, we still do not know the precise mechanisms of the lactic acid adaptation response in yeast and, consequently, lack effective approaches for improving its lactic acid tolerance. To enhance our understanding of the adaptation response, we screened for S. cerevisiae genes that confer enhanced lactic acid resistance when present in multiple copies and identified the transcriptional factor Haa1 as conferring resistance to toxic levels of lactic acid when overexpressed...
June 2014: Applied and Environmental Microbiology
keyword
keyword
28596
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"