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Acidic stress yeast

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https://www.readbyqxmd.com/read/28639705/cloning-and-characterization-of-iron-superoxide-dismutase-in-antarctic-yeast-strain-rhodotorula-mucilaginosa-an5
#1
Guangfeng Kan, Hua Wen, Xiaofei Wang, Ting Zhou, Cuijuan Shi
A novel superoxide dismutase gene from Antarctic yeast Rhodotorula mucilaginosa AN5 was cloned, sequenced, and then expressed in Escherichia coli. The R. mucilaginosa AN5 SOD (RmFeSOD) gene was 639 bp open reading frame in length, which encoded a protein of 212 amino acids with a deduced molecular mass of 23.5 kDa and a pI of 7.89. RmFeSOD was identified as iron SOD type with a natural status of homodimer. The recombinant RmFeSOD showed good pH stability in the pH 1.0-9.0 after 1 h incubation. Meanwhile, it was found to behave relatively high thermostability, and maintained more than 80% activity at 50 °C for 1 h...
June 22, 2017: Journal of Basic Microbiology
https://www.readbyqxmd.com/read/28633312/the-influence-of-dekkera-bruxellensis-on-the-transcriptome-of-saccharomyces-cerevisiae-and-on-the-aromatic-profile-of-synthetic-wine-must
#2
Janez Kosel, Neža Cadež, Dorit Schuller, Laura Carreto, Ricardo Franco-Duarte, Peter Raspor
A double compartment membrane system was constructed in order to systematically study possible microbial interactions between yeasts Saccharomyces cerevisiae and Dekkera bruxellensis and their impact on wine aroma. The presence of D. bruxellensis induced 77 transcripts of S. cerevisiae. These were mostly of unknown function; however, some were involved in thiamine biosynthesis and in amino acid and polyamine transport, suggesting a competitive relationship between the two yeast species. Among the transcripts with no biological function, 14 of them were found to be the members of the PAU gene family that is associated with response to anaerobiosis stress...
June 1, 2017: FEMS Yeast Research
https://www.readbyqxmd.com/read/28631015/ask-yeast-how-to-burn-your-fats-lessons-learned-from-the-metabolic-adaptation-to-salt-stress
#3
REVIEW
Amparo Pascual-Ahuir, Sara Manzanares-Estreder, Alba Timón-Gómez, Markus Proft
Here, we review and update the recent advances in the metabolic control during the adaptive response of budding yeast to hyperosmotic and salt stress, which is one of the best understood signaling events at the molecular level. This environmental stress can be easily applied and hence has been exploited in the past to generate an impressively detailed and comprehensive model of cellular adaptation. It is clear now that this stress modulates a great number of different physiological functions of the cell, which altogether contribute to cellular survival and adaptation...
June 19, 2017: Current Genetics
https://www.readbyqxmd.com/read/28625541/plant-derived-antifungal-compounds-trigger-a-common-transcriptional-response
#4
Zhe Wang
Understanding the mechanism of action of antifungal drugs is vital for better control of mycosis, which kills >1.3 million lives every year thus remains a major health problem worldwide. In this study, we investigate the activities of three different categories of plant-derived antifungal compounds (resveratrol, honokiol and osthole) via transcriptomics and bioinformatics analysis, with the goal of discovering the common Mode-of-Action (MoA) at molecular level. The result shows that a common transcriptional response (72 gene are up-regulated while 10 genes are down-regulated, commonly) are triggered by above representative antifungal compounds in Schizosaccharomyces pombe (S...
June 15, 2017: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/28623136/arabidopsis-inositol-polyphosphate-kinase-atipk2%C3%AE-is-phosphorylated-by-cpk4-and-positively-modulates-aba-signaling
#5
Peng Wang, Qiaofeng Yang, Sihong Sang, Yao Chen, Yujiao Zhong, Zhaoyun Wei
Arabidopsis inositol polyphosphate kinase 2β (AtIpk2β) has multiple functions in plant development and in responding to abiotic stress. Although some related clues suggested a potential role of AtIpk2β in ABA signaling, the defined evidence was still lack. Here we discovered that a key ABA signaling component calcium-dependent protein kinase 4 (CPK4) can interact with AtIpk2β under ABA treated conditions through affinity purification and mass spectrometry detection. The interaction between CPK4 and AtIpk2β were further confirmed by yeast two hybrid and bimolecular fluorescence complementation assays...
June 13, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28620360/cytr-homolog-of-pectobacterium-carotovorum-subsp-carotovorum-controls-air-liquid-biofilm-formation-by-regulating-multiple-genes-involved-in-cellulose-production-c-di-gmp-signaling-motility-and-type-iii-secretion-system-in-response-to-nutritional-and-environmental
#6
M M Haque, M M H Oliver, Kamrun Nahar, Mohammad Z Alam, Hisae Hirata, Shinji Tsuyumu
Pectobacterium carotovorum subsp. carotovorum [Pcc (formerly Erwinia carotovora subsp. carotovora)] PC1 causes soft-rot disease in a wide variety of plant species by secreting multiple pathogenicity-related traits. In this study, regulatory mechanism of air-liquid (AL) biofilm formation was studied using a cytR homolog gene deletion mutant (ΔcytR) of Pcc PC1. Compared to the wild type (Pcc PC1), the ΔcytR mutant produced fragile and significantly (P < 0.001) lower amounts of AL biofilm on salt-optimized broth plus 2% glycerol (SOBG), yeast peptone dextrose adenine, and also on King's B at 27°C after 72 h incubation in static condition...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28606754/deletion-of-acetate-transporter-gene-ady2-improved-tolerance-of-saccharomyces-cerevisiae-against-multiple-stresses-and-enhanced-ethanol-production-in-the-presence-of-acetic-acid
#7
Mingming Zhang, Keyu Zhang, Muhammad Aamer Mehmood, Zongbao Kent Zhao, Fengwu Bai, Xinqing Zhao
The aim of this work was to study the effects of deleting acetate transporter gene ADY2 on growth and fermentation of Saccharomyces cerevisiae in the presence of inhibitors. Comparative transcriptome analysis revealed that three genes encoding plasma membrane carboxylic acid transporters, especially ADY2, were significantly downregulated under the zinc sulfate addition condition in the presence of acetic acid stress, and the deletion of ADY2 improved growth of S. cerevisiae under acetic acid, ethanol and hydrogen peroxide stresses...
June 1, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28602696/the-medicago-truncatula-r2r3-myb-transcription-factor-gene-mtmybs1-enhances-salinity-tolerance-when-constitutively-expressed-in-arabidopsis-thaliana
#8
Wei Dong, Yuguang Song, Zhong Zhao, Nian Wei Qiu, Xijiang Liu, Weihua Guo
MYB-type proteins are known to participate in many stress responses, although their role in legumes is still less clear. Here, the isolation and characterization of MtMYBS1, an R2R3 MYB gene isolated from the model legume Medicago truncatula, is described. MtMYBS1 transcription was inducible by NaCl, polyethylene glycol or abscisic acid (ABA). When tested in yeast, its product was shown to have transactivational activity. The constitutive expression of MtMYBS1 in Arabidopsis thaliana seedlings mitigated the restriction on root growth imposed by either salinity or osmotic stress and raised their sensitivity to ABA...
June 8, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28600551/epe1-contributes-to-activation-of-ampk-by-promoting-phosphorylation-of-ampk-alpha-subunit-ssp2
#9
Yongyi Chen, Xiaoyue Hu, Chao Guo, Yao Yu, Hong Lu
AMP-activated protein kinase (AMPK) is a pivotal cellular energy sensor. It is activated by stresses that cause depletion of energy and initiates adaptive responses by regulating metabolism balance. AMPK forms αβγ heterotrimer. In fission yeast, activation of AMPK mainly depends on the phosphorylation of AMPKα subunit Ssp2 at Thr(189) by upstream kinase Ssp1. However, not much is known about the regulation of this process. In this study, we identified Epe1 as a novel positive regulator of AMPK. Epe1, a jmjC-domain-containing protein, is best-known as a negative regulator of heterochromatin spreading...
June 9, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28588114/two-glycerol-3-phosphate-dehydrogenases-from-chlamydomonas-have-distinct-roles-in-lipid-metabolism
#10
Thomas Driver, Drupad K Trivedi, Owen A McIntosh, Andrew P Dean, Royston Goodacre, Jon K Pittman
The metabolism of glycerol-3-phosphate (G3P) is important for environmental stress responses by eukaryotic microalgae. G3P is an essential precursor for glycerolipid synthesis and the accumulation of triacylglycerol (TAG) in response to nutrient starvation. G3P dehydrogenase (GPDH) mediates G3P synthesis but the roles of specific GPDH isoforms are currently poorly understood. Of the five GPDH enzymes in the model alga Chlamydomonas reinhardtii, GPD2 and GPD3 were shown to be induced by nutrient starvation and/or salt stress...
June 6, 2017: Plant Physiology
https://www.readbyqxmd.com/read/28587992/the-isolation-and-functional-characterization-of-three-liverwort-genes-encoding-cinnamate-4-hydroxylase
#11
Xin-Yan Liu, Hai-Na Yu, Shuai Gao, Yi-Feng Wu, Ai-Xia Cheng, Hong-Xiang Lou
The plant phenylpropanoid pathway is responsible for the synthesis of a wide variety of secondary metabolites. The second step in phenylpropanoid synthesis is carried out by the cytochrome P450 monooxygenase enzyme cinnamate 4-hydroxylase (C4H), which catalyzes the p-hydroxylation of trans-cinnamic acid to p-coumarate. Genes encoding C4H have been characterized in many vascular plant species, but as yet not in any bryophyte species. Here, a survey of the transcriptome sequences of four liverwort species was able to identify eight putative C4Hs...
May 26, 2017: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/28574606/sfp1-and-rtg3-reciprocally-modulate-carbon-source-conditional-stress-adaptation-in-the-pathogenic-yeast-candida-albicans
#12
Stavroula L Kastora, Carmen Herrero-de-Dios, Gabriela M Alvelar, Carol A Munro, Alistair J P Brown
The pathogenicity of the clinically important yeast, Candida albicans, is dependent on robust responses to host-imposed stresses. These stress responses have generally been dissected in vitro at 30°C on artificial growth media that do not mimic host niches. Yet host inputs, such as changes in carbon source or temperature, are known to affect C. albicans stress adaptation. Therefore, we performed screens to identify novel regulators that promote stress resistance during growth on a physiologically relevant carboxylic acid and at elevated temperatures...
June 2, 2017: Molecular Microbiology
https://www.readbyqxmd.com/read/28573339/heterologous-expression-of-the%C3%A2-carrot-hsp17-7-gene-increased-growth-cell-viability-and-protein-solubility-in-transformed-yeast-saccharomyces-cerevisiae-under-heat-cold-acid-and-osmotic-stress-conditions
#13
Eunhye Ko, Minhye Kim, Yunho Park, Yeh-Jin Ahn
In industrial fermentation of yeast (Saccharomyces cerevisiae), culture conditions are often modified from the optimal growth conditions of the cells to maintain large-scale cultures and/or to increase recombinant protein production. However, altered growth conditions can be stressful to yeast cells resulting in reduced cell growth and viability. In this study, a small heat shock protein gene from carrot (Daucus carota L.), Hsp17.7, was inserted into the yeast genome via homologous recombination to increase tolerance to stress conditions that can occur during industrial culture...
June 1, 2017: Current Microbiology
https://www.readbyqxmd.com/read/28572457/a-central-role-for-triacylglycerol-in-membrane-lipid-turnover-and-plant-survival-under-stress
#14
Jilian Fan, Linhui Yu, Changcheng Xu
Neutral lipid metabolism is a key aspect of intracellular homeostasis and energy balance and plays a vital role in cell survival under adverse conditions including nutrient deprivation in yeast and mammals, but the role of triacylglycerol (TAG) metabolism in plant stress response remains largely unknown. By thoroughly characterizing mutants defective in SUGAR-DEPENDENT1 (SDP1) triacylglycerol lipase or PEROXISOMAL ABC TRANSPORTER 1 (PXA1), here we show that TAG is a key intermediate in the mobilization of fatty acids from membrane lipids for peroxisomal ß-oxidation under prolonged dark treatment...
June 1, 2017: Plant Physiology
https://www.readbyqxmd.com/read/28558063/amino-acid-metabolites-that-regulate-g-protein-signaling-during-osmotic-stress
#15
James P Shellhammer, Elizabeth Morin-Kensicki, Jacob P Matson, Guowei Yin, Daniel G Isom, Sharon L Campbell, Robert P Mohney, Henrik G Dohlman
All cells respond to osmotic stress by implementing molecular signaling events to protect the organism. Failure to properly adapt can lead to pathologies such as hypertension and ischemia-reperfusion injury. Mitogen-activated protein kinases (MAPKs) are activated in response to osmotic stress, as well as by signals acting through G protein-coupled receptors (GPCRs). For proper adaptation, the action of these kinases must be coordinated. To identify second messengers of stress adaptation, we conducted a mass spectrometry-based global metabolomics profiling analysis, quantifying nearly 300 metabolites in the yeast S...
May 2017: PLoS Genetics
https://www.readbyqxmd.com/read/28536588/a-novel-wheat-c-bzip-gene-tabzip14-b-participates-in-salt-and-freezing-tolerance-in-transgenic-plants
#16
Lina Zhang, Lichao Zhang, Chuan Xia, Lifeng Gao, Chenyang Hao, Guangyao Zhao, Jizeng Jia, Xiuying Kong
The group C-bZIP transcription factors (TFs) are involved in diverse biological processes, such as the regulation of seed storage protein (SSP) production and the responses to pathogen challenge and abiotic stress. However, our knowledge of the abiotic functions of group C-bZIP genes in wheat remains limited. Here, we present the function of a novel TabZIP14-B gene in wheat. This gene belongs to the group C-bZIP TFs and contains six exons and five introns; three haplotypes were identified among accessions of tetraploid and hexaploid wheat...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28521568/prion-based-memory-of-heat-stress-in-yeast
#17
Tatiana A Chernova, Yury O Chernoff, Keith D Wilkinson
Amyloids and amyloid-based prions are self-perpetuating protein aggregates which can spread by converting a normal protein of the same sequence into a prion form. They are associated with diseases in humans and mammals, and control heritable traits in yeast and other fungi. Some amyloids are implicated in biologically beneficial processes. As prion formation generates reproducible memory of a conformational change, prions can be considered as molecular memory devices.  We have demonstrated that in yeast, stress-inducible cytoskeleton-associated protein Lsb2 forms a metastable prion in response to high temperature...
May 4, 2017: Prion
https://www.readbyqxmd.com/read/28503907/proteomics-analysis-for-asymmetric-inheritance-of-preexisting-proteins-between-mother-and-daughter-cells-in-budding-yeast
#18
Mitsuhiro Okada, Shunta Kusunoki, Yuko Ishibashi, Keiji Kito
In budding yeast, a mother cell can produce a finite number of daughter cells over its life. The accumulation of a variety of types of damaged components has an impact on the aging process. Asymmetrical inheritance during cell division causes these aberrant intracellular constituents to be retained in mother cells and prevents them from segregating to daughter cells. However, the understanding of asymmetrical inheritance of individual proteins that are damaged or old age, and their relevance to the aging process, has been limited...
May 15, 2017: Genes to Cells: Devoted to Molecular & Cellular Mechanisms
https://www.readbyqxmd.com/read/28497532/arabidopsis-thaliana-epoxide-hydrolase1-ateh1-is-a-cytosolic-epoxide-hydrolase-involved-in-the-synthesis-of-poly-hydroxylated-cutin-monomers
#19
Emmanuelle Pineau, Lin Xu, Hugues Renault, Adrien Trolet, Nicolas Navrot, Pascaline Ullmann, Bertrand Légeret, Gaëtan Verdier, Fred Beisson, Franck Pinot
Epoxide hydrolases (EHs) are present in all living organisms. They have been extensively characterized in mammals; however, their biological functions in plants have not been demonstrated. Based on in silico analysis, we identified AtEH1 (At3g05600), a putative Arabidopsis thaliana epoxide hydrolase possibly involved in cutin monomer synthesis. We expressed AtEH1 in yeast and studied its localization in vivo. We also analyzed the composition of cutin from A. thaliana lines in which this gene was knocked out...
May 12, 2017: New Phytologist
https://www.readbyqxmd.com/read/28488916/observing-the-fate-of-trna-and-its-modifications-by-nucleic-acid-isotope-labeling-mass-spectrometry-nail-ms
#20
Matthias Heiss, Valentin F Reichle, Stefanie Kellner
RNA in yeast, especially rRNA and tRNA are heavily modified to fulfill their function in protein translation. Using biosynthetic stable isotope labeled internal standards we quantified 12 modified nucleosides in tRNA from S. cerevisiae over 24 hours. We observed different quantities of modified nucleosides in dependence of the growth phase. To elucidate the underlying mechanism of the observed tRNA modification profile adaptation, it is necessary to distinguish the pre-existing tRNA pool and its modifications from newly-synthesized tRNAs...
May 10, 2017: RNA Biology
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