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Acidic stress yeast

Karamat Mohammad, Paméla Dakik, Younes Medkour, Mélissa McAuley, Darya Mitrofanova, Vladimir I Titorenko
The concentrations of some key metabolic intermediates play essential roles in regulating the longevity of the chronologically aging yeast Saccharomyces cerevisiae . These key metabolites are detected by certain ligand-specific protein sensors that respond to concentration changes of the key metabolites by altering the efficiencies of longevity-defining cellular processes. The concentrations of the key metabolites that affect yeast chronological aging are controlled spatially and temporally. Here, we analyze mechanisms through which the spatiotemporal dynamics of changes in the concentrations of the key metabolites influence yeast chronological lifespan...
March 15, 2018: International Journal of Molecular Sciences
Leandro Ferreira Moreno, Vania Aparecida Vicente, Sybren de Hoog
Black yeasts (BY) comprise a group of polyextremotolerant fungi, mainly belonging to the order Chaetothyriales, which are capable of colonizing a wide range of extreme environments. The tolerance to hostile habitats can be explained by their intrinsic ability to survive under acidic, alkaline, and toxic conditions, high temperature, low nutrient availability, and osmotic and mechanical stress. Occasionally, some species can cause human chromoblastomycosis, a chronic subcutaneous infection, as well as disseminated or cerebral phaeohyphomycosis...
April 1, 2018: Medical Mycology: Official Publication of the International Society for Human and Animal Mycology
Chieun Song, Jimin Lee, Taeyoon Kim, Jong Chan Hong, Chae Oh Lim
Under normal growth conditions, Arabidopsis VOZ1 interacts with DREB2C and acts as a transcriptional repressor by reducing DNA binding of DREB2C. Under heat stress conditions, VOZ1 is degraded by ubiquitination, and DREB2C, which is freed from VOZ1, functions as a transcription activator. To investigate the mechanism by which the DEHYDRATION-RESPONSIVE ELEMENT-BINDING FACTOR 2C (DREB2C)-dependent signaling cascade regulates heat stress (HS) responses, we performed a yeast two-hybrid screening using the DREB2C APETALA2 (AP2) DNA-binding domain as the bait against a cDNA library derived from Arabidopsis...
March 13, 2018: Planta
Hao Ji, Xinyao Lu, Hong Zong, Bin Zhuge
γ-aminobutyric acid (GABA) is an important non-protein amino acid involved in the response to various environmental stresses in plant cells. The objectives of this study was to test the hypothesis that intracellular accumulation of GABA improves osmotic tolerance in the unconventional yeast Candida glycerinogenes. In C. glycerinogenes, the expression of UGA4 encoding GABA-specific permease is highly induced by hyperosmotic stress. Exogenous GABA application enhanced intracellular GABA accumulation and promoted cell growth under hyperosmotic conditions...
March 12, 2018: Journal of General and Applied Microbiology
Asma Timoumi, Stéphane E Guillouet, Carole Molina-Jouve, Luc Fillaudeau, Nathalie Gorret
The yeast Yarrowia lipolytica is an industrially important microorganism with distinctive physiological and metabolic characteristics. A variety of external factors (e.g., pH, temperature, and nutrient availability) influences the behavior of the yeast and may act as stress conditions which the cells must withstand and adapt. In this mini review, the impacts of environmental factors on the morphology and metabolite production by Y. lipolytica are summarized. In this regard, detailed insights into the effectors involved in the dimorphic transition of Y...
March 9, 2018: Applied Microbiology and Biotechnology
Keiichirou Nemoto, Makiko Kagawa, Akira Nozawa, Yoshinori Hasegawa, Minoru Hayashi, Kenichiro Imai, Kentaro Tomii, Tatsuya Sawasaki
Abscisic acid (ABA) is the main phytohormone involved in abiotic stress response and its adaptation, and is a candidate agrichemical. Consequently, several agonists of ABA have been developed using the yeast two-hybrid system. Here, we describe a novel cell-free-based drug screening approach for the development and validation of ABA receptor agonists. Biochemical validation of this approach between 14 ABA receptors (PYR/PYL/RCARs) and 7 type 2C-A protein phosphatases (PP2CAs) revealed the same interactions as those of previous proteome data, except for nine new interactions...
March 9, 2018: Scientific Reports
Valter Miotto Alessio, Natale Cavaçana, Luíza Lane de Barros Dantas, Nayoung Lee, Carlos Takeshi Hotta, Takato Imaizumi, Marcelo Menossi
Ethylene is a phytohormone involved in the regulation of several aspects of plant development as well as in responses to biotic and abiotic stress. The effects of exogenous application of ethylene to sugarcane plants are well characterized: growth inhibition of immature internodes and stimulation of sucrose accumulation. However, the molecular network underlying the control of ethylene biosynthesis in sugarcane remains largely unknown. The chemical reaction catalyzed by 1-amynociclopropane-1-carboxylic acid synthase (ACS) is an important rate limiting-step that regulates ethylene production in plants...
March 3, 2018: Journal of Experimental Botany
Guaiqiang Chai, Chunlian Li, Feng Xu, Yang Li, Xue Shi, Yong Wang, Zhonghua Wang
BACKGROUND: The cuticle covers the surface of the polysaccharide cell wall of leaf epidermal cells and forms an essential diffusion barrier between the plant and the environment. The cuticle is composed of cutin and wax. Cuticular wax plays an important role in the survival of plants by serving as the interface between plants and their biotic and abiotic environments, especially restricting nonstomatal water loss. Leaf cuticular waxes of hexaploid wheat at the seedling stage mainly consist of primary alcohols, aldehydes, fatty acids, alkane and esters...
March 5, 2018: BMC Plant Biology
Yovanny Izquierdo, Satish Kulasekaran, Pablo Benito, Bran López, Ruth Marcos, Tomás Cascón, Mats Hamberg, Carmen Castresana
Stress adaptation and translational regulation was studied using noxy7 (non-responding to oxylipins7) from a series of Arabidopsis thaliana mutants. We identified the noxy7 mutation in At1g64790, which encodes a homolog of the yeast translational regulator GENERAL CONTROL NON-DEREPRESSIBLE1 (GCN1) that activates the GCN2 kinase; GCN2 in turn phosphorylates the α subunit of the translation initiation factor eIF2. This regulatory circuit is conserved in yeast and mammals, in which phosphorylated eIF2α (P-eIF2α) facilitates stress adaptation by inhibiting protein synthesis...
March 2, 2018: Plant, Cell & Environment
Osvaldo Filipe, David De Vleesschauwer, Ashley Haeck, Kristof Demeestere, Monica Höfte
Sucrose non-fermenting-1-related protein kinase-1 (SnRK1) belongs to a family of evolutionary conserved kinases with orthologs in all eukaryotes, ranging from yeasts (SnF1) to mammals (AMP-Activated kinase). These kinases sense energy deficits caused by nutrient limitation or stress and coordinate the required adaptations to maintain energy homeostasis and survival. In plants, SnRK1 is a global regulator of plant metabolism and is also involved in abiotic stress responses. Its role in the response to biotic stress, however, is only starting to be uncovered...
March 1, 2018: Scientific Reports
António Rego, Katrina F Cooper, Justin Snider, Yusuf A Hannun, Vítor Costa, Manuela Côrte-Real, Susana Rodrigues Chaves
Changes in sphingolipid metabolism have been linked to modulation of cell fate in both yeast and mammalian cells. We previously assessed the role of sphingolipids in cell death regulation using a well characterized yeast model of acetic acid-induced regulated cell death, finding that Isc1p, inositol phosphosphingolipid phospholipase C, plays a pro-death role in this process. Indeed, isc1∆ mutants exhibited a higher resistance to acetic acid associated with reduced mitochondrial alterations. Here, we show that Isc1p is regulated by Sch9p under acetic acid stress, since both single and double mutants lacking Isc1p or/and Sch9p have the same resistant phenotype, and SCH9 deletion leads to a higher retention of Isc1p in the endoplasmic reticulum upon acetic acid exposure...
February 26, 2018: Biochimica et Biophysica Acta
Dustin C Ernst, Diana M Downs
A variety of metabolic deficiencies and human diseases arise from the disruption of mitochondrial enzymes and/or loss of mitochondrial DNA. Mounting evidence shows that eukaryotes have conserved enzymes that prevent the accumulation of reactive metabolites that cause stress inside the mitochondrion. 2-Aminoacrylate is a reactive enamine generated by pyridoxal 5'-phosphate-dependent α,β-eliminases as an obligatory intermediate in the breakdown of serine. In prokaryotes, members of the broadly conserved RidA family (PF14588) prevent metabolic stress by deaminating 2-aminoacrylate to pyruvate...
February 27, 2018: MBio
Jorge Mejía-Barajas, Rocío Montoya-Pérez, Salvador Manzo-Avalos, Christian Cortés-Rojo, Héctor Riveros-Rosas, Carlos Cervantes, Alfredo Saavedra-Molina
Membrane fatty acid composition has an important role in yeast stress resistance, particularly in temperature tolerance. Most studies investigating temperature and membrane fatty acids use the yeast Saccharomyces cerevisiae without considering other yeasts, such as Kluyveromyces marxianus, which has physiological differences and industrial advantages with respect to Saccharomyces cerevisiae. One of the primary traits of Kluyveromyces marxianus is its thermotolerance. The effect of fatty acid addition (oleic acid, linoleic acid, linolenic acid, and araquidic acid) on the thermotolerance of the Kluyveromyces marxianus strain SLP1 was evaluated...
February 22, 2018: FEMS Microbiology Letters
Nadia Maria Berterame, Francesca Martani, Danilo Porro, Paola Branduardi
The yeast Saccharomyces cerevisiae is widely used as a cell factory for the biotechnological production of various industrial products. During these processes, yeasts meet different kinds of stressors that often cause oxidative stress and thus impair cell growth. Therefore, the development of robust strains is indispensable to improve production, yield and productivity of fermentative processes. Copper plays a key role in the response to oxidative stress, as cofactor of the cytosolic superoxide dismutase (Sod1) and being contained in metallochaperone and metallothioneines with antioxidant properties...
February 22, 2018: Metabolic Engineering
C Heit, S J Martin, F Yang, D L Inglis
AIMS: Volatile acidity production along with gene expression patterns, encoding enzymes involved in both acetic acid production and utilization, were investigated to relate gene expression patterns to the production of undesired volatile acidity during Icewine fermentation. METHODS AND RESULTS: Icewine juice and diluted Icewine juice were fermented using the Saccharomyces cerevisiae wine yeast K1-V1116. Acetic acid production increased 6-fold during the Icewine fermentation versus the diluted juice condition, while ethyl acetate production increased 2...
February 14, 2018: Journal of Applied Microbiology
Hemanth Noothalapati, Ryo Ikarashi, Keita Iwasaki, Tatsuro Nishida, Tomohiro Kaino, Keisuke Yoshikiyo, Keiji Terao, Daisuke Nakata, Naoko Ikuta, Masahiro Ando, Hiro-O Hamaguchi, Makoto Kawamukai, Tatsuyuki Yamamoto
α-lipoic acid (ALA) is an essential cofactor for many enzyme complexes in aerobic metabolism, especially in mitochondria of eukaryotic cells where respiration takes place. It also has excellent anti-oxidative properties. The acid has two stereo-isomers, R- and S- lipoic acid (R-LA and S-LA), but only the R-LA has biological significance and is exclusively produced in our body. A mutant strain of fission yeast, Δdps1, cannot synthesize coenzyme Q10, which is essential during yeast respiration, leading to oxidative stress...
February 6, 2018: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
Kathy Parisi, Thomas M A Shafee, Pedro Quimbar, Nicole L van der Weerden, Mark R Bleackley, Marilyn A Anderson
Plant defensins are an extensive family of small cysteine rich proteins characterised by a conserved cysteine stabilised alpha beta protein fold which resembles the structure of insect and vertebrate defensins. However, secondary structure and disulphide topology indicates two independent superfamilies of defensins with similar structures that have arisen via an extreme case of convergent evolution. Defensins from plants and insects belong to the cis-defensin superfamily whereas mammalian defensins belong to the trans-defensin superfamily...
February 9, 2018: Seminars in Cell & Developmental Biology
Yulan Shi, Xiule Yue, Lizhe An
Ω-3 fatty acid desaturases (FADs) are thought to contribute to plant stress tolerance mainly through the C18:3-induced membrane stabilization, but the comprehensive analysis of their roles in stress adaptation is lacking. Here, we isolated a microsomal ω-3 FAD gene (CbFAD3) from a cryophyte (Chorispora bungeana), and elucidated its functions in stress tolerance. CbFAD3, exhibiting highest identity to Arabidopsis AtFAD3, was up-regulated by abiotic stresses. Its functionality was verified by heterogonous expression in yeast...
February 8, 2018: Journal of Experimental Botany
Caia D S Duncan, María Rodríguez-López, Phil Ruis, Jürg Bähler, Juan Mata
Eukaryotes respond to amino acid starvation by enhancing the translation of mRNAs encoding b-ZIP family transcription factors (GCN4 in Saccharomyces cerevisiae and ATF4 in mammals), which launch transcriptional programs to counter this stress. This pathway involves phosphorylation of the eIF2 translation factor by Gcn2-protein kinases and is regulated by upstream ORFs (uORFs) in the GCN4/ATF4 5' leaders. Here, we present evidence that the transcription factors that mediate this response are not evolutionarily conserved...
February 5, 2018: Proceedings of the National Academy of Sciences of the United States of America
Praveen Kumar Allu, Yerranna Boggula, Srinivasu Karri, Adinarayana Marada, Thanuja Krishnamoorthy, Naresh Babu V Sepuri
Cells across evolution employ reversible oxidative modification of methionine and cysteine amino acids within proteins to regulate responses to redox stress. Previously we have shown that mitochondrial localized methionine sulfoxide reductase (Mxr2) reversibly regulates oxidized yeast Mge1 (yMge1), a co-chaperone of Hsp70/Ssc1 to maintain protein homeostasis during oxidative stress. However, the specificity and the conservation of the reversible methionine oxidation mechanism in higher eukaryotes is debatable as human GrpEL1 (hGrpEL1) unlike its homolog yMge1 harbors two methionine residues and multiple cysteines besides the mammalian mitochondria hosting R and S types of Mxrs/Msrs...
February 9, 2018: Scientific Reports
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