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Acidic stress yeast

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https://www.readbyqxmd.com/read/28712705/engineering-of-global-regulators-and-cell-surface-properties-toward-enhancing-stress-tolerance-in-saccharomyces-cerevisiae
#1
REVIEW
Kouichi Kuroda, Mitsuyoshi Ueda
Microbial cell factories are subject to various stresses, leading to the reductions of metabolic activity and bioproduction efficiency. Therefore, the development of stress-tolerant microorganisms is important for improving bio-production efficiency. Recently, modifications of cell surface properties and master regulators have been shown to be effective approaches for enhancing stress tolerance. The cell surface is an attractive target owing to its interactions with the environment and its role in transmitting environmental information...
July 13, 2017: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/28710262/med15b-regulates-acid-stress-response-and-tolerance-in-candida-glabrata
#2
Yanli Qi, Hui Liu, Jiayin Yu, Xiulai Chen, Liming Liu
Candida glabrata is a promising producer of organic acids. To elucidate the physiological function of the Mediator tail subunit Med15B in the response to low pH stress, we constructed a deletion strain, med15BΔ, and overexpression strain, HTUΔ/CgMED15B Deletion of MED15B caused biomass production, glucose consumption rate, and cell viability to decrease by 28.3%, 31.7%, and 26.5%, respectively, compared with those of the parent strain (HTUΔ) at pH 2.0. Expression of lipid metabolism-related genes was significantly downregulated in the med15BΔ strain, whereas key genes of ergosterol biosynthesis showed abnormal upregulation...
July 14, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28702017/acetic-acid-causes-endoplasmic-reticulum-stress-and-induces-the-unfolded-protein-response-in-saccharomyces-cerevisiae
#3
Nozomi Kawazoe, Yukio Kimata, Shingo Izawa
Since acetic acid inhibits the growth and fermentation ability of Saccharomyces cerevisiae, it is one of the practical hindrances to the efficient production of bioethanol from a lignocellulosic biomass. Although extensive information is available on yeast response to acetic acid stress, the involvement of endoplasmic reticulum (ER) and unfolded protein response (UPR) has not been addressed. We herein demonstrated that acetic acid causes ER stress and induces the UPR. The accumulation of misfolded proteins in the ER and activation of Ire1p and Hac1p, an ER-stress sensor and ER stress-responsive transcription factor, respectively, were induced by a treatment with acetic acid stress (>0...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28681139/a-novel-ap2-erf-family-transcription-factor-from-glycine-soja-gserf71-is-a-dna-binding-protein-that-positively-regulates-alkaline-stress-tolerance-in-arabidopsis
#4
Yang Yu, Xiangbo Duan, Xiaodong Ding, Chao Chen, Dan Zhu, Kuide Yin, Lei Cao, Xuewei Song, Pinghui Zhu, Qiang Li, Zaib Un Nisa, Jiyang Yu, Jianying Du, Yu Song, Huiqing Li, Beidong Liu, Yanming Zhu
Here we first found that GsERF71, an ERF factor from wild soybean could increase plant alkaline stress tolerance by up-regulating H+-ATPase and by modifing the accumulation of Auxin. Alkaline soils are widely distributed all over the world and greatly limit plant growth and development. In our previous transcriptome analyses, we have identified several ERF (ethylene-responsive factor) genes that responded strongly to bicarbonate stress in the roots of wild soybean G07256 (Glycine soja). In this study, we cloned and functionally characterized one of the genes, GsERF71...
July 2017: Plant Molecular Biology
https://www.readbyqxmd.com/read/28667821/an-apple-cipk-protein-kinase-targets-a-novel-residue-of-areb-transcription-factor-for-aba-dependent-phosphorylation
#5
Qi-Jun Ma, Mei-Hong Sun, Jing Lu, Ya-Jing Liu, Chun-Xiang You, Yu-Jin Hao
Phytohormone abscisic acid (ABA) regulates many important processes in plants. It is a major molecule facilitating signal transduction during the abiotic stress response. In this study, an ABA-inducible transcription factor gene, MdAREB2, was identified in apple. Transgenic analysis was performed to characterize its function in ABA sensitivity. Overexpression of the MdAREB2 gene increased ABA sensitivity in the transgenic apple compared with the WT control. In addition, it was found that the protein MdAREB2 was phosphorylated at a novel site Thr(411) in response to ABA...
July 1, 2017: Plant, Cell & Environment
https://www.readbyqxmd.com/read/28659185/the-genome-wide-transcription-response-to-telomerase-deficiency-in-the-thermotolerant-yeast-hansenula-polymorpha-dl-1
#6
Alexey V Beletsky, Alexander N Malyavko, Maria V Sukhanova, Eugenia S Mardanova, Maria I Zvereva, Olga A Petrova, Yulia Yu Parfenova, Maria P Rubtsova, Andrey V Mardanov, Olga I Lavrik, Olga A Dontsova, Nikolai V Ravin
BACKGROUND: In the course of replication of eukaryotic chromosomes, the telomere length is maintained due to activity of telomerase, the ribonucleoprotein reverse transcriptase. Abolishing telomerase function causes progressive shortening of telomeres and, ultimately, cell cycle arrest and replicative senescence. To better understand the cellular response to telomerase deficiency, we performed a transcriptomic study for the thermotolerant methylotrophic yeast Hansenula polymorpha DL-1 lacking telomerase activity...
June 28, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28654660/isolation-and-characterization-of-gmmybj3-an-r2r3-myb-transcription-factor-that-affects-isoflavonoids-biosynthesis-in-soybean
#7
Mingzhu Zhao, Tianliang Wang, Ping Wu, Wenyun Guo, Liantai Su, Ying Wang, Yajing Liu, Fan Yan, Qingyu Wang
Isoflavonoids are secondary metabolites that play a variety of roles in plant-microbe interactions and plant defenses against abiotic stresses. Here we report a new MYB transcription factor (TF) gene, GmMYBJ3, that is involved in the isoflavonoids biosynthesis. The GmMYBJ3 gene is 1,002 bp long and encodes a protein of 333 amino acids. Amino acid sequence analysis showed that GmMYBJ3 is a typical R2R3 MYB TF. Yeast expression experiment demonstrated that GmMYBJ3 has its transcription activity in the nucleus and is transiently expressed in onion epidermal cells...
2017: PloS One
https://www.readbyqxmd.com/read/28652237/the-candidate-antimalarial-drug-mmv665909-causes-oxygen-dependent-mrna-mistranslation-and-synergises-with-quinoline-derived-antimalarials
#8
Cindy Vallières, Simon V Avery
To cope with growing resistance to current antimalarials, new drugs with novel modes of action are urgently needed. Molecules targeting protein synthesis appear to be promising candidates. We identified a compound (MMV665909) from the MMV Malaria Box of candidate antimalarials that could produce synergistic growth inhibition with the aminoglycoside antibiotic paromomycin, suggesting a possible action of the compound in mRNA mistranslation. This mechanism of action was substantiated with the yeast cell model using available reporters of mistranslation and other genetic tools...
June 26, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28649255/overexpression-of-the-peat1-elicitor-gene-from-alternaria-tenuissima-improves-drought-tolerance-in-rice-plants-via-interaction-with-a-myo-inositol-oxygenase
#9
Fachao Shi, Yijie Dong, Yi Zhang, Xiufeng Yang, Dewen Qiu
Abiotic stresses, especially drought, seriously threaten cereal crops yields and quality. In this study, we observed that the rice plants of overexpression the Alternariatenuissima PeaT1 gene showed enhanced drought stress tolerance and increased the survival rate following a drought treatment. In PeaT1-overexpressing (PeaT1OE) plants, abscisic acid and chlorophyll content significantly increased, while the malondialdehyde (MDA) content decreased compared with the wild-type plants. Additionally, we confirmed that the transcript levels of drought-responsive genes, including OsAM1, OsLP2, and OsDST, were prominently lower in the PeaT1OE plants...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28639705/cloning-and-characterization-of-iron-superoxide-dismutase-in-antarctic-yeast-strain-rhodotorula-mucilaginosa-an5
#10
Guangfeng Kan, Hua Wen, Xiaofei Wang, Ting Zhou, Cuijuan Shi
A novel superoxide dismutase gene from Antarctic yeast Rhodotorula mucilaginosa AN5 was cloned, sequenced, and then expressed in Escherichia coli. The R. mucilaginosa AN5 SOD (RmFeSOD) gene was 639 bp open reading frame in length, which encoded a protein of 212 amino acids with a deduced molecular mass of 23.5 kDa and a pI of 7.89. RmFeSOD was identified as iron SOD type with a natural status of homodimer. The recombinant RmFeSOD showed good pH stability in the pH 1.0-9.0 after 1 h incubation. Meanwhile, it was found to behave relatively high thermostability, and maintained more than 80% activity at 50 °C for 1 h...
June 22, 2017: Journal of Basic Microbiology
https://www.readbyqxmd.com/read/28633312/the-influence-of-dekkera-bruxellensis-on-the-transcriptome-of-saccharomyces-cerevisiae-and-on-the-aromatic-profile-of-synthetic-wine-must
#11
Janez Kosel, Neža Cadež, Dorit Schuller, Laura Carreto, Ricardo Franco-Duarte, Peter Raspor
A double compartment membrane system was constructed in order to systematically study possible microbial interactions between yeasts Saccharomyces cerevisiae and Dekkera bruxellensis and their impact on wine aroma. The presence of D. bruxellensis induced 77 transcripts of S. cerevisiae. These were mostly of unknown function; however, some were involved in thiamine biosynthesis and in amino acid and polyamine transport, suggesting a competitive relationship between the two yeast species. Among the transcripts with no biological function, 14 of them were found to be the members of the PAU gene family that is associated with response to anaerobiosis stress...
June 1, 2017: FEMS Yeast Research
https://www.readbyqxmd.com/read/28631015/ask-yeast-how-to-burn-your-fats-lessons-learned-from-the-metabolic-adaptation-to-salt-stress
#12
REVIEW
Amparo Pascual-Ahuir, Sara Manzanares-Estreder, Alba Timón-Gómez, Markus Proft
Here, we review and update the recent advances in the metabolic control during the adaptive response of budding yeast to hyperosmotic and salt stress, which is one of the best understood signaling events at the molecular level. This environmental stress can be easily applied and hence has been exploited in the past to generate an impressively detailed and comprehensive model of cellular adaptation. It is clear now that this stress modulates a great number of different physiological functions of the cell, which altogether contribute to cellular survival and adaptation...
June 19, 2017: Current Genetics
https://www.readbyqxmd.com/read/28625541/plant-derived-antifungal-compounds-trigger-a-common-transcriptional-response
#13
Zhe Wang
Understanding the mechanism of action of antifungal drugs is vital for better control of mycosis, which kills >1.3 million lives every year thus remains a major health problem worldwide. In this study, we investigate the activities of three different categories of plant-derived antifungal compounds (resveratrol, honokiol and osthole) via transcriptomics and bioinformatics analysis, with the goal of discovering the common Mode-of-Action (MoA) at molecular level. The result shows that a common transcriptional response (72 gene are up-regulated while 10 genes are down-regulated, commonly) are triggered by above representative antifungal compounds in Schizosaccharomyces pombe (S...
June 15, 2017: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/28623136/arabidopsis-inositol-polyphosphate-kinase-atipk2%C3%AE-is-phosphorylated-by-cpk4-and-positively-modulates-aba-signaling
#14
Peng Wang, Qiaofeng Yang, Sihong Sang, Yao Chen, Yujiao Zhong, Zhaoyun Wei
Arabidopsis inositol polyphosphate kinase 2β (AtIpk2β) has multiple functions in plant development and in responding to abiotic stress. Although some related clues suggested a potential role of AtIpk2β in ABA signaling, the defined evidence was still lack. Here we discovered that a key ABA signaling component calcium-dependent protein kinase 4 (CPK4) can interact with AtIpk2β under ABA treated conditions through affinity purification and mass spectrometry detection. The interaction between CPK4 and AtIpk2β were further confirmed by yeast two hybrid and bimolecular fluorescence complementation assays...
August 19, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28620360/cytr-homolog-of-pectobacterium-carotovorum-subsp-carotovorum-controls-air-liquid-biofilm-formation-by-regulating-multiple-genes-involved-in-cellulose-production-c-di-gmp-signaling-motility-and-type-iii-secretion-system-in-response-to-nutritional-and-environmental
#15
M M Haque, M M H Oliver, Kamrun Nahar, Mohammad Z Alam, Hisae Hirata, Shinji Tsuyumu
Pectobacterium carotovorum subsp. carotovorum [Pcc (formerly Erwinia carotovora subsp. carotovora)] PC1 causes soft-rot disease in a wide variety of plant species by secreting multiple pathogenicity-related traits. In this study, regulatory mechanism of air-liquid (AL) biofilm formation was studied using a cytR homolog gene deletion mutant (ΔcytR) of Pcc PC1. Compared to the wild type (Pcc PC1), the ΔcytR mutant produced fragile and significantly (P < 0.001) lower amounts of AL biofilm on salt-optimized broth plus 2% glycerol (SOBG), yeast peptone dextrose adenine, and also on King's B at 27°C after 72 h incubation in static condition...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28606754/deletion-of-acetate-transporter-gene-ady2-improved-tolerance-of-saccharomyces-cerevisiae-against-multiple-stresses-and-enhanced-ethanol-production-in-the-presence-of-acetic-acid
#16
Mingming Zhang, Keyu Zhang, Muhammad Aamer Mehmood, Zongbao Kent Zhao, Fengwu Bai, Xinqing Zhao
The aim of this work was to study the effects of deleting acetate transporter gene ADY2 on growth and fermentation of Saccharomyces cerevisiae in the presence of inhibitors. Comparative transcriptome analysis revealed that three genes encoding plasma membrane carboxylic acid transporters, especially ADY2, were significantly downregulated under the zinc sulfate addition condition in the presence of acetic acid stress, and the deletion of ADY2 improved growth of S. cerevisiae under acetic acid, ethanol and hydrogen peroxide stresses...
June 1, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28602696/the-medicago-truncatula-r2r3-myb-transcription-factor-gene-mtmybs1-enhances-salinity-tolerance-when-constitutively-expressed-in-arabidopsis-thaliana
#17
Wei Dong, Yuguang Song, Zhong Zhao, Nian Wei Qiu, Xijiang Liu, Weihua Guo
MYB-type proteins are known to participate in many stress responses, although their role in legumes is still less clear. Here, the isolation and characterization of MtMYBS1, an R2R3 MYB gene isolated from the model legume Medicago truncatula, is described. MtMYBS1 transcription was inducible by NaCl, polyethylene glycol or abscisic acid (ABA). When tested in yeast, its product was shown to have transactivational activity. The constitutive expression of MtMYBS1 in Arabidopsis thaliana seedlings mitigated the restriction on root growth imposed by either salinity or osmotic stress and raised their sensitivity to ABA...
August 19, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28600551/epe1-contributes-to-activation-of-ampk-by-promoting-phosphorylation-of-ampk-alpha-subunit-ssp2
#18
Yongyi Chen, Xiaoyue Hu, Chao Guo, Yao Yu, Hong Lu
AMP-activated protein kinase (AMPK) is a pivotal cellular energy sensor. It is activated by stresses that cause depletion of energy and initiates adaptive responses by regulating metabolism balance. AMPK forms αβγ heterotrimer. In fission yeast, activation of AMPK mainly depends on the phosphorylation of AMPKα subunit Ssp2 at Thr(189) by upstream kinase Ssp1. However, not much is known about the regulation of this process. In this study, we identified Epe1 as a novel positive regulator of AMPK. Epe1, a jmjC-domain-containing protein, is best-known as a negative regulator of heterochromatin spreading...
June 9, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28588114/two-glycerol-3-phosphate-dehydrogenases-from-chlamydomonas-have-distinct-roles-in-lipid-metabolism
#19
Thomas Driver, Drupad K Trivedi, Owen A McIntosh, Andrew P Dean, Royston Goodacre, Jon K Pittman
The metabolism of glycerol-3-phosphate (G3P) is important for environmental stress responses by eukaryotic microalgae. G3P is an essential precursor for glycerolipid synthesis and the accumulation of triacylglycerol (TAG) in response to nutrient starvation. G3P dehydrogenase (GPDH) mediates G3P synthesis but the roles of specific GPDH isoforms are currently poorly understood. Of the five GPDH enzymes in the model alga Chlamydomonas reinhardtii, GPD2 and GPD3 were shown to be induced by nutrient starvation and/or salt stress...
June 6, 2017: Plant Physiology
https://www.readbyqxmd.com/read/28587992/the-isolation-and-functional-characterization-of-three-liverwort-genes-encoding-cinnamate-4-hydroxylase
#20
Xin-Yan Liu, Hai-Na Yu, Shuai Gao, Yi-Feng Wu, Ai-Xia Cheng, Hong-Xiang Lou
The plant phenylpropanoid pathway is responsible for the synthesis of a wide variety of secondary metabolites. The second step in phenylpropanoid synthesis is carried out by the cytochrome P450 monooxygenase enzyme cinnamate 4-hydroxylase (C4H), which catalyzes the p-hydroxylation of trans-cinnamic acid to p-coumarate. Genes encoding C4H have been characterized in many vascular plant species, but as yet not in any bryophyte species. Here, a survey of the transcriptome sequences of four liverwort species was able to identify eight putative C4Hs...
May 26, 2017: Plant Physiology and Biochemistry: PPB
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