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Acidic stress yeast

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https://www.readbyqxmd.com/read/27913740/a-%C3%AE-ketoacyl-coa-synthase-is-involved-in-rice-leaf-cuticular-wax-synthesis-and-requires-a-cer2-like-protein-as-a-cofactor
#1
Xiaochen Wang, Yuanyuan Guan, Du Zhang, Xiangbai Dong, Lihong Tian, Le Qing Qu
Cuticular waxes are complex mixtures of very-long-chain fatty acids (VLCFAs) and their derivatives, forming a natural barrier on aerial surfaces of terrestrial plants against biotic and abiotic stresses. In VLCFA biosynthesis, β-ketoacyl-CoA synthase (KCS) is the key enzyme, catalyzing the first reaction in fatty acid elongation and determining substrate specificity. We isolated a rice (Oryza sativa L.) wax crystal-sparse leaf 4 (WSL4) gene using a map-based cloning strategy. WSL4 is predicted to encode a KCS, a homolog of Arabidopsis CER6...
December 2, 2016: Plant Physiology
https://www.readbyqxmd.com/read/27900876/effects-of-production-methods-and-protective-ingredients-on-the-viability-of-probiotic-lactobacillus-rhamnosus-r0011-in-air-dried-alginate-beads
#2
Claude P Champagne, Yves Raymond, Yves Arcand
The goal of this study was to use a microencapsulation technology to prepare air-dried concentrated cultures of Lactobacillus rhamnosus R0011. The cultures were microencapsulated in alginate beads, which were added to a growth medium to allow cell multiplication inside the matrix; the beads were recovered, dipped in protective solutions, and air-dried. The effects of fermentation technology and of the composition of the protective solutions on subsequent survival during air-drying were examined. The cells prepared under a constant pH of 6...
August 26, 2016: Canadian Journal of Microbiology
https://www.readbyqxmd.com/read/27896382/the-mechanism-of-improved-intracellular-organic-selenium-and-glutathione-contents-in-selenium-enriched-candida-utilis-by-acid-stress
#3
Gao-Chuan Zhang, Da-Hui Wang, Dong-Hua Wang, Gong-Yuan Wei
Batch culture of Candida utilis CCTCC M 209298 for the preparation of selenium (Se)-enriched yeast was carried out under different pH conditions, and maximal intracellular organic Se and glutathione (GSH) contents were obtained in a moderate acid stress environment (pH 3.5). In order to elucidate the physiological mechanism of improved performance of Se-enriched yeast by acid stress, assays of the key enzymes involved in GSH biosynthesis and determinations of energy supply and regeneration were performed. The results indicated that moderate acid stress increased the activity of γ-glutamylcysteine synthetase and the ratios of NADH/NAD(+) and ATP/ADP, although no significant changes in intracellular pH were observed...
November 28, 2016: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/27886402/analysis-of-paracoccidioides-lutzii-mitochondria-a-proteomic-approach
#4
L Casaletti, P S Lima, L N Lima, C L Borges, S N Báo, A M Bailão, C M A Bailão
The genus Paracoccidioides is composed of thermal dimorphic fungi, causative agents of paracoccidioidomycosis, one of the most frequent systemic mycoses in Latin America. Mitochondria have sophisticated machinery for ATP production, which involves metabolic pathways such as citric acid and glyoxylate cycles, electron transport chain and oxidative phosphorylation. In addition, this organelle performs a variety of functions in the cell, working as an exceptional metabolic signaling center that contributes to cellular stress responses, as autophagy and apoptosis in eukaryotic organisms...
November 25, 2016: Yeast
https://www.readbyqxmd.com/read/27883312/time-course-analysis-of-gene-expression-during-the-saccharomyces-cerevisiae-hypoxic-response
#5
Nasrine Bendjilali, Samuel MacLeon, Gurmannat Kalra, Stephen D Willis, A K M Nawshad Hossian, Erica Avery, Olivia Wojtowicz, Mark J Hickman
Many cells experience hypoxia, or low oxygen, and respond by dramatically altering gene expression. In the yeast S. cerevisiae, genes that respond are required for many oxygen-dependent cellular processes, such as respiration, biosynthesis and redox regulation. To more fully characterize the global response to hypoxia, we exposed yeast to hypoxic conditions, extracted RNA at different times and performed RNA-seq analysis. Time-course statistical analysis revealed hundreds of genes that changed expression by up to 550-fold...
November 9, 2016: G3: Genes—Genomes—Genetics
https://www.readbyqxmd.com/read/27878508/calcium-supplementation-abates-the-inhibition-effects-of-acetic-acid-on-saccharomyces-cerevisiae
#6
Hongwei Zhao, Jingyuan Li, Jiming Wang, Xin Xu, Mo Xian, Huizhou Liu, Haibo Zhang
The toxic level of acetic acid could be released during the pretreatment of lignocellulosic biomass, and an economical method was reported to minimize the acidic stress on the fermentation of Saccharomyces cerevisiae by cation supplementation. A dose-dependent protection of Ca(2+) was monitored, and the optimal concentration of Ca(2+) was 8 mM under 4.5 g/L acetic acid stress. The activities of catalase and superoxide dismutase of yeast cells supplemented with optimal Ca(2+) increased by 18.6 and 27.3 %, respectively, coupling with an obvious decrease of reactive oxygen species content...
November 22, 2016: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/27868313/nutrient-dependent-increased-dendritic-arborization-of-somatosensory-neurons
#7
Kaori Watanabe, Yuki Furumizo, Tadao Usui, Yukako Hattori, Tadashi Uemura
Suboptimal nutrition imposes developmental constraints on infant animals, which marshal adaptive responses to eventually become mature adults. Such responses are mounted at multiple levels from systemic to cellular. At the cellular level, the underlying mechanisms of cell proliferation control have been intensively studied. However, less is known about how growth of postmitotic and morphologically complex cells, such as neurons, is controlled by nutritional status. We address this question using Class I and Class IV dendritic arborization neurons in Drosophila larvae...
November 21, 2016: Genes to Cells: Devoted to Molecular & Cellular Mechanisms
https://www.readbyqxmd.com/read/27868205/antioxidant-properties-and-global-metabolite-screening-of-the-probiotic-yeast-saccharomyces-cerevisiae-var-boulardii
#8
Suprama Datta, David J Timson, Uday S Annapure
BACKGROUND: Saccharomyces cerevisiae var. boulardii is the only yeast species with probiotic properties. It is considered to have therapeutic significance in gastro-intestinal disorders. In this paper, a comparative physiological study between this yeast and Saccharomyces cerevisiae (BY4742) was performed by evaluating two prominent traits of probiotic species, responses to different stress conditions and antioxidant capacity. A global metabolite profile was also developed in order to identify which therapeutically important secondary metabolites are produced...
November 21, 2016: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/27860167/jrwrky2-and-jrwrky7-of-juglans-regia-mediate-responses-to-abiotic-stresses-and-abscisic-acid-by-formation-of-homodimers-and-interaction
#9
Guiyan Yang, Wenhui Zhang, Zexing Liu, A-Yijiamali Yi-Maer, Meizhi Zhai, Zhenggang Xu
[Backgroud] WRKY transcription factors (TFs) belong to a large protein family that is involved in diverse developmental processes and abiotic stress responses. Currently, there is little understanding of the role of WRKY TFs in regulatory mechanisms in plants, especially in the protein-protein interactions that are essential for biological regulatory functions and networks. [Methods] In the present study, the yeast one-hybrid, yeast two-hybrid, transient expression and quantitative RT-PCR were applied to investigate the potential characters of two WRKY proteins from Juglans regia, JrWRKY2 (GenBank No...
November 11, 2016: Plant Biology
https://www.readbyqxmd.com/read/27835736/citeromyces-matritensis-m37-is-a-salt-tolerant-yeast-that-produces-ethanol-from-salted-algae
#10
Masahiko Okai, Ayako Betsuno, Ayaka Shirao, Nobuo Obara, Kotaro Suzuki, Toshinori Takei, Masachika Takashio, Masami Ishida, Naoto Urano
Algae are referred to as a third-generation biomass for ethanol production. However, salinity treatment is a problem that needs to be solved, because algal hydrolysates often contain high salt. Here, we isolated the salt-tolerant ethanol-producing yeast Citeromyces matritensis M37 from the east coast of Miura Peninsula in Japan. This yeast grew under osmotic stress conditions (20% NaCl or 60% glucose). It produced 6.55 g/L ethanol from YPD medium containing 15% NaCl after 48 h, and the ethanol accumulation was observed even at 20% NaCl...
August 26, 2016: Canadian Journal of Microbiology
https://www.readbyqxmd.com/read/27815194/evolutionary-engineering-reveals-divergent-paths-when-yeast-is-adapted-to-different-acidic-environments
#11
Eugene Fletcher, Amir Feizi, Markus M M Bisschops, Björn M Hallström, Sakda Khoomrung, Verena Siewers, Jens Nielsen
Tolerance of yeast to acid stress is important for many industrial processes including organic acid production. Therefore, elucidating the molecular basis of long term adaptation to acidic environments will be beneficial for engineering production strains to thrive under such harsh conditions. Previous studies using gene expression analysis have suggested that both organic and inorganic acids display similar responses during short term exposure to acidic conditions. However, biological mechanisms that will lead to long term adaptation of yeast to acidic conditions remains unknown and whether these mechanisms will be similar for tolerance to both organic and inorganic acids is yet to be explored...
November 1, 2016: Metabolic Engineering
https://www.readbyqxmd.com/read/27814402/characterization-of-cghif%C3%AE-like-a-novel-bhlh-pas-transcription-factor-family-member-and-its-role-under-hypoxia-stress-in-the-pacific-oyster-crassostrea-gigas
#12
Ting Wang, Jie Meng, Li Li, Guofan Zhang
Hypoxia-inducible factor (HIF), a critical member of the basic-helix-loop-helix (bHLH)-containing Per-Arnt-Sim (PAS) protein family, is a master transcription factor involved in maintaining oxygen homeostasis. In the present study, we isolated and characterized a novel bHLH-PAS family member, CgHIFα-like gene, from the Pacific oyster Crassostrea gigas, and determined its importance during hypoxia stress. The 3020-bp CgHIFα-like cDNA encoded a protein of 888 amino acids. The predicted CgHIFα-like amino acid sequence was conserved in the N-terminal bHLH, PAS, and PAC domains (but not in the C-terminal domain) and was most closely related to the HIF family in the bHLH-PAS protein phylogenic tree...
2016: PloS One
https://www.readbyqxmd.com/read/27798688/adaptive-response-of-listeria-monocytogenes-to-heat-salinity-and-low-ph-after-habituation-on-cherry-tomatoes-and-lettuce-leaves
#13
Sofia V Poimenidou, Danai-Natalia Chatzithoma, George-John Nychas, Panagiotis N Skandamis
Pathogens found on fresh produce may encounter low temperatures, high acidity and limited nutrient availability. The aim of this study was to evaluate the effect of habituation of Listeria monocytogenes on cherry tomatoes or lettuce leaves on its subsequent response to inhibitory levels of acid, osmotic and heat stress. Habituation was performed by inoculating lettuce coupons, whole cherry tomatoes or tryptic soy broth (TSB) with a three-strains composite of L. monocytogenes, which were further incubated at 5°C for 24 hours or 5 days...
2016: PloS One
https://www.readbyqxmd.com/read/27793829/overexpression-of-ole1-enhances-cytoplasmic-membrane-stability-and-confers-resistance-to-cadmium-in-saccharomyces-cerevisiae
#14
Zhijia Fang, Zhongxiang Chen, Song Wang, Ping Shi, Yuhu Shen, Youshang Zhang, Junhua Xiao, Zhiwei Huang
: Heavy metal cadmium is widely used and released into the environment, posing a severe threat to crops and humans. Saccharomyces cerevisiae is one of the most commonly used organisms in the investigation of environmental metal toxicity. We investigated cadmium stress and the adaptive mechanisms of yeast by screening a genome-wide essential genes overexpression library. A candidate gene, OLE1, encodes a delta-9 desaturase and was associated with high anti-cadmium activity. The results demonstrate that the expression of OLE1 is positively correlated with cadmium stress tolerance and induction was Mga2p and Spt23p (important regulatory factors of OLE1) independent...
October 28, 2016: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/27783562/empirical-verification-of-evolutionary-theories-of-aging
#15
Pavlo Kyryakov, Alejandra Gomez-Perez, Anastasia Glebov, Nimara Asbah, Luigi Bruno, Carolynne Meunier, Tatiana Iouk, Vladimir I Titorenko
We recently selected 3 long-lived mutant strains of Saccharomyces cerevisiae by a lasting exposure to exogenous lithocholic acid. Each mutant strain can maintain the extended chronological lifespan after numerous passages in medium without lithocholic acid. In this study, we used these long-lived yeast mutants for empirical verification of evolutionary theories of aging. We provide evidence that the dominant polygenic trait extending longevity of each of these mutants 1) does not affect such key features of early-life fitness as the exponential growth rate, efficacy of post-exponential growth and fecundity; and 2) enhances such features of early-life fitness as susceptibility to chronic exogenous stresses, and the resistance to apoptotic and liponecrotic forms of programmed cell death...
October 25, 2016: Aging
https://www.readbyqxmd.com/read/27782169/valproate-inhibits-map-kinase-signalling-and-cell-cycle-progression-in-s-cerevisiae
#16
Kristelle Desfossés-Baron, Ian Hammond-Martel, Antoine Simoneau, Adnane Sellam, Stephen Roberts, Hugo Wurtele
The mechanism of action of valproate (VPA), a widely prescribed short chain fatty acid with anticonvulsant and anticancer properties, remains poorly understood. Here, the yeast Saccharomyces cerevisiae was used as model to investigate the biological consequences of VPA exposure. We found that low pH strongly potentiates VPA-induced growth inhibition. Transcriptional profiling revealed that under these conditions, VPA modulates the expression of genes involved in diverse cellular processes including protein folding, cell wall organisation, sexual reproduction, and cell cycle progression...
October 26, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27777448/implementing-principles-of-traditional-concentrated-grape-must-fermentation-to-the-production-of-new-generation-balsamic-vinegars-starter-selection-and-effectiveness
#17
Sofia Lalou, Angela Capece, Fani Th Mantzouridou, Patrizia Romano, Maria Z Tsimidou
In an effort to implement principles of traditional concentrated grape must fermentation to the production of new generation balsamic vinegars (BVs), the specific goals of the study were the isolation and molecular identification of the predominant yeasts in concentrated grape must (cv. Xinomavro), their technological characterization and the evaluation of the fermentative aptitude of the selected strains. Tolerance against 5-hydroxymethyl-furfural (HMF) and furfural, acetic acid and glucose concentration was examined by appropriate methods and tests...
September 2016: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/27771580/an-rrm-containing-mei2-like-mct1-plays-a-negative-role-in-the-seed-germination-and-seedling-growth-of-arabidopsis-thaliana-in-the-presence-of-aba
#18
Lili Gu, Hyun Ju Jung, Kyung Jin Kwak, Sy Nguyen Dinh, Yeon-Ok Kim, Hunseung Kang
Despite an increasing understanding of the essential role of the Mei2 gene encoding an RNA-binding protein (RBP) in premeiotic DNA synthesis and meiosis in yeasts and animals, the functional roles of the mei2-like genes in plant growth and development are largely unknown. Contrary to other mei2-like RBPs that contain three RNA-recognition motifs (RRMs), the mei2 C-terminal RRM only (MCT) is unique in that it harbors only the last C-terminal RRM. Although MCTs have been implicated to play important roles in plants, their functional roles in stress responses as well as plant growth and development are still unknown...
October 13, 2016: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/27752498/antioxidant-and-neuroprotective-effects-of-doenjang-prepared-with-rhizopus-pichia-and-bacillus
#19
Su Jin Kang, Ji Yeon Seo, Kye Man Cho, Chang Kwon Lee, Jeong Hwan Kim, Jong-Sang Kim
A new type of doenjang was manufactured by mixing soaked soybean, koji (Rhizopus oryzae), cheonggukjang (Bacillus amyloliquefaciens MJ1-4 and B. amyloliquefaciens EMD17), and Pichia farinosa SY80 as a yeast, salt, and water, followed by fermentation with koji that was made by fermenting whole wheat with R. oryzae. The mixed culture doenjang was designed to have a more palatable flavor and stronger biological activities than the conventional product. The extract of mixed culture doenjang showed higher antioxidant activity than the commercial doenjang as evaluated by the ferric reducing antioxidant power assay although it was not significantly different from the commercial product in 2,2-diphenyl-1-picrylhydrazyl and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) radical scavenging activities...
September 2016: Preventive Nutrition and Food Science
https://www.readbyqxmd.com/read/27734932/combined-transcriptomics-and-chemical-genetics-reveal-molecular-mode-of-action-of-valproic-acid-an-anticancer-molecule-using-budding-yeast-model
#20
Upendarrao Golla, Deepthi Joseph, Raghuvir Singh Tomar
Valproic acid (VA) is a pharmacologically important histone deacetylase inhibitor that recently garnered attention as an anticancer agent. Since the molecular mechanisms behind the multiple effects of VA are unclear, this study was aimed to unravel the comprehensive cellular processes affected by VA and its molecular targets in vivo using budding yeast as a model organism. Interestingly, genome-wide transcriptome analysis of cells treated with VA showed differential regulation of 30% of the genome. Functional enrichment analysis of VA transcriptome evidenced alteration of various cellular processes including cell cycle, cell wall biogenesis, DNA repair, ion homeostasis, metabolism, stress response, transport and ribosomal biogenesis, etc...
October 13, 2016: Scientific Reports
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