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Acidic stress yeast

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https://www.readbyqxmd.com/read/28338710/the-wrky-transcription-factors-ptrwrky18-and-ptrwrky35-promote-melampsora-resistance-in-populus
#1
Yuanzhong Jiang, Li Guo, Xiaodong Ma, Xin Zhao, Bo Jiao, Chaofeng Li, Keming Luo
WRKY transcription factors play important roles in response to diverse environmental stresses, but exact functions of these proteins in poplar defense are still largely unknown. In a previous study, we have shown that poplar WRKY89 is induced by salicylic acid (SA) treatment and plays an important role in resistance against fungi in transgenic poplars. Here, we determined an increase in transcript levels of Group IIa WRKY members in transgenic poplars overexpressing WRKY89 using quantitative real-time polymerase chain reaction analysis...
February 15, 2017: Tree Physiology
https://www.readbyqxmd.com/read/28337841/yeast-aquaporin-regulation-by-4-hydroxynonenal-is-implicated-in-oxidative-stress-response
#2
Claudia Rodrigues, Ivana Tartaro Bujak, Branka Mihaljević, Graça Soveral, Ana Cipak Gasparovic
Reactive oxygen species, especially hydrogen peroxide (H2 O2 ), contribute to functional molecular impairment and cellular damage, but also are necessary in normal cellular metabolism, and in low doses play stimulatory role in cell proliferation and stress resistance. In parallel, reactive aldehydes such as 4-hydroxynonenal (HNE), are lipid peroxidation breakdown products which also contribute to regulation of numerous cellular processes. Recently, channeling of H2 O2 by some mammalian aquaporin isoforms has been reported and suggested to contribute to aquaporin involvement in cancer malignancies, although the mechanism by which these membrane water channels are implicated in oxidative stress is not clear...
March 24, 2017: IUBMB Life
https://www.readbyqxmd.com/read/28330319/roles-of-plant-hormones-and-anti-apoptosis-genes-during-drought-stress-in-rice-oryza-sativa-l
#3
Mohammad Ubaidillah, Fika Ayu Safitri, Jun-Hyeon Jo, Sang-Kyu Lee, Adil Hussain, Bong-Gyu Mun, Il Kyung Chung, Byung-Wook Yun, Kyung-Min Kim
We previously identified the rice (Oryza sativa) senescence-associated gene OsSAP which encodes a highly conserved protein involved in anti-apoptotic activity. This novel Bax suppressor-related gene regulates tolerance to multiple stresses in yeast. Here, we show the effects of drought stress on leaf and root tissues of plants over-expressing OsSAP in relation to the levels of phytohormones, abscisic acid (ABA), jasmonic acid (JA), indole-3-carboxylic acid (ICA), gibberellic acid (GA3), and zeatin. Results showed that rice plants over-expressing SAP were tolerant to drought stress compared to wild type and the plants over-expressing AtBI-1, which is a homolog of the human Bax inhibitor-1 in Arabidopsis...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28326092/a-novel-soybean-erf-transcription-factor-gmerf113-increases-resistance-to-phytophthora-sojae-infection-in-soybean
#4
Yuanling Zhao, Xin Chang, Dongyue Qi, Lidong Dong, Guangjin Wang, Sujie Fan, Liangyu Jiang, Qun Cheng, Xi Chen, Dan Han, Pengfei Xu, Shuzhen Zhang
Phytophthora root and stem rot of soybean caused by the oomycete Phytophthora sojae, is a destructive disease worldwide. Ethylene response factors (ERFs) play important roles in regulating plant biotic and abiotic stress tolerance. In this study, a new ERF gene, GmERF113, was isolated from the highly resistant soybean 'Suinong 10.' Sequence analysis suggested that the protein encoded by GmERF113 contained a conserved AP2/ERF domain of 58 amino acid and belonged to the B-4 subgroup of the ERF subfamily. Expression of GmERF113 was significantly induced by P...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28321934/multi-layered-control-of-peroxisomal-activity-upon-salt-stress-in-saccharomyces-cerevisiae
#5
Sara Manzanares-Estreder, Joan Espí-Bardisa, Benito Alarcón, Amparo Pascual-Ahuir, Markus Proft
Peroxisomes are dynamic organelles and the sole location for fatty acid β-oxidation in yeast cells. Here we report that peroxisomal function is crucial for the adaptation to salt stress, especially upon sugar limitation. Upon stress, multiple layers of control regulate the activity and the number of peroxisomes. Activated Hog1 MAP kinase triggers the induction of genes encoding enzymes for fatty acid activation, peroxisomal import and β-oxidation through the Adr1 transcriptional activator, which transiently associates with genes encoding fatty acid metabolic enzymes in a stress- and Hog1-dependent manner...
March 21, 2017: Molecular Microbiology
https://www.readbyqxmd.com/read/28314628/petit-high-pressure-carbon-dioxide-stress-increases-synthesis-of-s-adenosylmethionine-and-phosphatidylcholine-in-yeast-saccharomyces-cerevisiae
#6
Liyuan Niu, Kazuki Nomura, Hitoshi Iwahashi, Hiroyuki Matsuoka, Satoshi Kawachi, Yoshihisa Suzuki, Katsuhiro Tamura
Petit-High Pressure Carbon Dioxide (p-HPCD) is a promising nonthermal technology for foods pasteurization. Cluster analysis of gene expression profiles of Saccharomyces cerevisiae exposed to various stresses exhibited that gene expression profile for p-HPCD stress (0.5MPa, 25°C) was grouped into a cluster including profiles for Sodium Dodecyl Sulfate and Roundup herbicide. Both are detergents that can disorder membrane structurally and functionally, which suggests that cell membrane may be a target of p-HPCD stress to cause cell growth inhibition...
March 8, 2017: Biophysical Chemistry
https://www.readbyqxmd.com/read/28304398/characterization-of-the-polyphenol-oxidase-gene-family-reveals-a-novel-microrna-involved-in-posttranscriptional-regulation-of-ppos-in-salvia-miltiorrhiza
#7
Caili Li, Dongqiao Li, Jiang Li, Fenjuan Shao, Shanfa Lu
Salvia miltiorrhiza is a well-known material of traditional Chinese medicine. Understanding the regulatory mechanisms of phenolic acid biosynthesis and metabolism are important for S. miltiorrhiza quality improvement. We report here that S. miltiorrhiza contains 19 polyphenol oxidases (PPOs), forming the largest PPO gene family in plant species to our knowledge. Analysis of gene structures and sequence features revealed the conservation and divergence of SmPPOs. SmPPOs were differentially expressed in plant tissues and eight of them were predominantly expressed in phloem and xylem, indicating that some SmPPOs are functionally redundant, whereas the others are associated with different physiological processes...
March 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28295166/engineering-oxidative-stress-defense-pathways-to-build-a-robust-lipid-production-platform-in-yarrowia-lipolytica
#8
Peng Xu, Kangjian Qiao, Gregory Stephanopoulos
Microbially-derived lipids have recently attracted renewed interests due to their broad applications in production of green diesels, cosmetic additives and oleochemicals. Metabolic engineering efforts have targeted a large portfolio of biosynthetic pathways to efficiently convert sugar to lipids in oleaginous yeast. In the engineered overproducing strains, endogenous cell metabolism typically generates harmful electrophilic molecules that compromise cell fitness and productivity. Lipids, particularly unsaturated fatty acids, are highly susceptible to oxygen radical attack and the resulting oxidative species are detrimental to cell metabolism and limit lipid productivity...
March 14, 2017: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/28294107/antifungal-mechanisms-of-zno-and-ag-nanoparticles-to-sclerotinia-homoeocarpa
#9
Junli Li, Hyunkyu Sang, Huiyuan Guo, James T Popko, Lili He, Jason C White, Om Parkash Dhankher, Geunhwa Jung, Baoshan Xing
Fungicides have extensively been used to effectively combat fungal diseases on a range of plant species, but resistance to multiple active ingredients has developed in pathogens such as Sclerotinia homoeocarpa, the causal agent of dollar spot on cool-season turfgrasses. Recently, ZnO and Ag nanoparticles (NPs) have received increased attention due to their antimicrobial activities. In this study, the NPs' toxicity and mechanisms of action were investigated as alternative antifungal agents against S. homoeocarpa isolates that varied in their resistance to demethylation inhibitor (DMI) fungicides...
April 18, 2017: Nanotechnology
https://www.readbyqxmd.com/read/28293217/mechanistic-insights-underlying-tolerance-to-acetic-acid-stress-in-vaginal-candida-glabrata-clinical-isolates
#10
Diana V Cunha, Sara B Salazar, Maria M Lopes, Nuno P Mira
During colonization of the vaginal tract Candida glabrata cells are challenged with the presence of acetic acid at a low pH, specially when dysbiosis occurs. To avoid exclusion from this niche C. glabrata cells are expected to evolve efficient adaptive responses to cope with this stress; however, these responses remain largely uncharacterized, especially in vaginal strains. In this work a cohort of 18 vaginal strains and 2 laboratory strains (CBS138 and KUE100) were phenotyped for their tolerance against inhibitory concentrations of acetic acid at pH 4...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28286511/bhbzip60-from-resurrection-plant-boea-hygrometrica-is-an-mrna-splicing-activated-endoplasmic-reticulum-stress-regulator-involved-in-drought-tolerance
#11
Bo Wang, Hong Du, Zhennan Zhang, Wenzhong Xu, Xin Deng
Adverse environmental conditions cause endoplasmic reticulum (ER) stress in plants. To mitigate ER stress damage, ER associated transcription factors and inositol-requiring enzyme-1 (IRE1)-mediated bZIP60 mRNA splicing are activated in plants. A drought-induced gene, encoding the ortholog of AtbZIP60, was identified in the resurrection plant Boea hygrometrica, termed BhbZIP60. In response to ER stress and dehydration, BhbZIP60 mRNA can be spliced to create a frame shift in the C terminus by the excision of 23b segment in a manner of its ortholog in other plants, thus translocating to the nucleus instead of the cytoplasm...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28285084/a-salt-stress-regulator-from-the-poplar-r2r3-myb-family-integrates-the-regulation-of-lateral-root-emergence-and-aba-signaling-to-mediate-salt-stress-tolerance-in-arabidopsis
#12
Qing Fang, Tianzhi Jiang, Liangxiang Xu, Hai Liu, Hui Mao, Xianqiang Wang, Bo Jiao, Yanjiao Duan, Qiong Wang, Qiannan Dong, Li Yang, Guozheng Tian, Chi Zhang, Yifeng Zhou, Xiaopeng Liu, Haiyang Wang, Di Fan, Bangjun Wang, Keming Luo
The roles of most MYB transcription factors (TFs) in the poplar remain unclear. Here, we demonstrate that PtrSSR1, a salt-stress-regulator in the Populus trichocarpa R2R3 MYB gene family, mediates the tolerance of transgenic Arabidopsis plants to salt stress. The transcripts of PtrSSR1 could be induced by salt stress rapidly in poplar. Subcellular localization and yeast assays indicated that PtrSSR1 encoded a nuclear protein with transactivation activity. The Arabidopsis transformants overexpressing PtrSSR1 clearly displayed lateral root emergence (LRE) inhibition compared with wild-type (Wt) under normal conditions; while upon NaCl treatment, the transformants showed improved tolerance, and the LRs emerged faster from salt-induced inhibition...
February 24, 2017: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/28282432/metabolic-crosstalk-between-membrane-and-storage-lipids-facilitates-heat-stress-management-in-schizosaccharomyces-pombe
#13
Mária Péter, Attila Glatz, Péter Gudmann, Imre Gombos, Zsolt Török, Ibolya Horváth, László Vígh, Gábor Balogh
Cell membranes actively participate in stress sensing and signalling. Here we present the first in-depth lipidomic analysis to characterize alterations in the fission yeast Schizosaccharomyces pombe in response to mild heat stress (HS). The lipidome was assessed by a simple one-step methanolic extraction. Genetic manipulations that altered triglyceride (TG) content in the absence or presence of HS gave rise to distinct lipidomic fingerprints for S. pombe. Cells unable to produce TG demonstrated long-lasting growth arrest and enhanced signalling lipid generation...
2017: PloS One
https://www.readbyqxmd.com/read/28278249/expression-of-c-5-sterol-desaturase-from-an-edible-mushroom-in-fisson-yeast-enhances-its-ethanol-and-thermotolerance
#14
Ayushi Kamthan, Mohan Kamthan, Asis Datta
Bioethanol is an environment friendly and renewable source of energy produced by the fermentation of agricultural raw material by a variety of microorganisms including yeast. Obtaining yeast strains that are tolerant to stresses like high levels of ethanol and high temperature is highly desirable as it reduces cost and increases yield during bioethanol production. Here, we report that heterologous expression of C-5 Sterol desaturase (FvC5SD)-an ergosterol biosynthesis enzyme from an edible mushroom Flammulina velutipes in fission yeast, not only imparts increased thermotolerance but also tolerance towards high ethanol concentration and low pH...
2017: PloS One
https://www.readbyqxmd.com/read/28270203/the-effects-of-torc-signal-interference-on-lipogenesis-in-the-oleaginous-yeast-trichosporon-oleaginosus
#15
Felix Bracharz, Veronika Redai, Kathrin Bach, Farah Qoura, Thomas Brück
BACKGROUND: Oleaginous organisms are a promising, renewable source of single cell oil. Lipid accumulation is mainly induced by limitation of nutrients such as nitrogen, phosphorus or sulfur. The oleaginous yeast Trichosporon oleaginosus accumulates up to 70% w/w lipid under nitrogen stress, while cultivation in non-limiting media only yields 9% w/w lipid. Uncoupling growth from lipid accumulation is key for the industrial process applicability of oleaginous yeasts. This study evaluates the effects of rapamycin on TOR specific signaling pathways associated with lipogenesis in Trichosporon oleaginosus for the first time...
March 7, 2017: BMC Biotechnology
https://www.readbyqxmd.com/read/28258766/an-account-of-fungal-14-3-3-proteins
#16
REVIEW
Ravinder Kumar
14-3-3s are a group of relatively low molecular weight, acidic, dimeric, protein(s) conserved from single-celled yeast to multicellular vertebrates including humans. Despite lacking catalytic activity, these proteins have been shown to be involved in multiple cellular processes. Apart from their role in normal cellular physiology, recently these proteins have been implicated in various medical consequences. In this present review, fungal 14-3-3 protein localization, interactions, transcription, regulation, their role in the diverse cellular process including DNA duplication, cell cycle, protein trafficking or secretion, apoptosis, autophagy, cell viability under stress, gene expression, spindle positioning, role in carbon metabolism have been discussed...
February 24, 2017: European Journal of Cell Biology
https://www.readbyqxmd.com/read/28236766/oxidative-stress-metabolomics-profiling-and-mechanism-of-local-anesthetic-induced-cell-death-in-yeast
#17
Cory H T Boone, Ryan A Grove, Dana Adamcova, Javier Seravalli, Jiri Adamec
The World Health Organization designates lidocaine as an essential medicine in healthcare, greatly increasing the probability of human exposure. Its use has been associated with ROS generation and neurotoxicity. Physiological and metabolomic alterations, and genetics leading to the clinically observed adverse effects have not been temporally characterized. To study alterations that may lead to these undesirable effects, Saccharomyces cerevisiae grown on aerobic carbon sources to stationary phase was assessed over 6h...
February 3, 2017: Redox Biology
https://www.readbyqxmd.com/read/28216580/impacts-of-dietary-phytochemicals-in-the-presence-and-absence-of-pesticides-on-longevity-of-honey-bees-apis-mellifera
#18
Ling-Hsiu Liao, Wen-Yen Wu, May R Berenbaum
Because certain flavonols and phenolic acids are found in pollen and nectar of most angiosperms, they are routinely ingested by Apis mellifera, the western honey bee. The flavonol quercetin and the phenolic acid p-coumaric acid are known to upregulate detoxification enzymes in adult bees; their presence or absence in the diet may thus affect the toxicity of ingested pesticides. We conducted a series of longevity assays with one-day-old adult workers to test if dietary phytochemicals enhance longevity and pesticide tolerance...
February 14, 2017: Insects
https://www.readbyqxmd.com/read/28212626/a-global-characterization-of-the-translational-and-transcriptional-programs-induced-by-methionine-restriction-through-ribosome-profiling-and-rna-seq
#19
Ke Zou, Qi Ouyang, Hao Li, Jiashun Zheng
BACKGROUND: Among twenty amino acids, methionine has a special role as it is coded by the translation initiation codon and methionyl-tRNAi (Met-tRNAi) is required for the assembly of the translation initiation complex. Thus methionine may play a special role in global gene regulation. Methionine has also been known to play important roles in cell growth, development, cancer, and aging. In this work, we characterize the translational and transcriptional programs induced by methionine restriction (MetR) and investigate the potential mechanisms through which methionine regulates gene expression, using the budding yeast S...
February 17, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28209995/rna-seq-based-transcriptomic-and-metabolomic-analysis-reveal-stress-responses-and-programmed-cell-death-induced-by-acetic-acid-in-saccharomyces-cerevisiae
#20
Yachen Dong, Jingjin Hu, Linlin Fan, Qihe Chen
As a typical harmful inhibitor in cellulosic hydrolyzates, acetic acid not only hinders bioethanol production, but also induces cell death in Saccharomyces cerevisiae. Herein, we conducted both transcriptomic and metabolomic analyses to investigate the global responses under acetic acid stress at different stages. There were 295 up-regulated and 427 down-regulated genes identified at more than two time points during acetic acid treatment (150 mM, pH 3.0). These differentially expressed genes (DEGs) were mainly involved in intracellular homeostasis, central metabolic pathway, transcription regulation, protein folding and stabilization, ubiquitin-dependent protein catabolic process, vesicle-mediated transport, protein synthesis, MAPK signaling pathways, cell cycle, programmed cell death, etc...
February 17, 2017: Scientific Reports
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