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Acidic stress yeast

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https://www.readbyqxmd.com/read/28099852/yeast-short-lived-actin-associated-protein-forms-a-metastable-prion-in-response-to-thermal-stress
#1
Tatiana A Chernova, Denis A Kiktev, Andrey V Romanyuk, John R Shanks, Oskar Laur, Moiez Ali, Abheek Ghosh, Dami Kim, Zhen Yang, Maggie Mang, Yury O Chernoff, Keith D Wilkinson
Self-perpetuating ordered protein aggregates (amyloids and prions) are associated with a variety of neurodegenerative disorders. Although environmental agents have been linked to certain amyloid diseases, the molecular basis of their action remains unclear. We have employed endogenous yeast prions as a model system to study environmental control of amyloid formation. A short-lived actin-associated yeast protein Lsb2 can trigger prion formation by other proteins in a mode regulated by the cytoskeleton and ubiquitin-dependent processes...
January 17, 2017: Cell Reports
https://www.readbyqxmd.com/read/28094115/oxidative-stress-and-antioxidant-response-in-a-thermotolerant-yeast
#2
Jorge A Mejía-Barajas, Rocío Montoya-Pérez, Rafael Salgado-Garciglia, Leopoldo Aguilera-Aguirre, Christian Cortés-Rojo, Ricardo Mejía-Zepeda, Melchor Arellano-Plaza, Alfredo Saavedra-Molina
Stress tolerance is a key attribute that must be considered when using yeast cells for industrial applications. High temperature is one factor that can cause stress in yeast. High environmental temperature in particular may exert a natural selection pressure to evolve yeasts into thermotolerant strains. In the present study, three yeasts (Saccharomyces cerevisiae, MC4, and Kluyveromyces marxianus, OFF1 and SLP1) isolated from hot environments were exposed to increased temperatures and were then compared with a laboratory yeast strain...
January 3, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28089773/mitochondrial-diseases-yeast-as-a-model-for-the-study-of-suppressors
#3
Silvia Francisci, Arianna Montanari
Mitochondrial (mt) tRNA gene mutations are an important cause of human morbidity and are associated with different syndromes. We have previously shown that the mitochondrial protein synthesis elongation factor EF-Tu and isolated sequences from the carboxy-terminal domain of yeast and human mt leucyl-tRNA synthetases (LeuRS), have a wide range of suppression capability among different yeast mt tRNA mutants having defective respiratory phenotype. Here we show that the rescuing capability can be restricted to a specific sequence of six amino acids from the carboxy-terminal domain of mt LeuRS...
January 12, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28086780/the-zygosaccharomyces-bailii-transcription-factor-haa1-is-required-for-acetic-acid-and-copper-stress-responses-suggesting-subfunctionalization-of-the-ancestral-bifunctional-protein-haa1-cup2
#4
Margarida Palma, Paulo Jorge Dias, Filipa de Canaveira Roque, Laura Luzia, Joana Fernandes Guerreiro, Isabel Sá-Correia
BACKGROUND: The food spoilage yeast species Zygosaccharomyces bailii exhibits an extraordinary capacity to tolerate weak acids, in particular acetic acid. In Saccharomyces cerevisiae, the transcription factor Haa1 (ScHaa1) is considered the main player in genomic expression reprogramming in response to acetic acid stress, but the role of its homologue in Z. bailii (ZbHaa1) is unknown. RESULTS: In this study it is demonstrated that ZbHaa1 is a ScHaa1 functional homologue by rescuing the acetic acid susceptibility phenotype of S...
January 13, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28070662/investigation-of-the-instability-and-low-water-kefir-grain-growth-during-an-industrial-water-kefir-fermentation-process
#5
David Laureys, Amandine Van Jean, Jean Dumont, Luc De Vuyst
A poorly performing industrial water kefir production process consisting of a first fermentation process, a rest period at low temperature, and a second fermentation process was characterized to elucidate the causes of its low water kefir grain growth and instability. The frozen-stored water kefir grain inoculum was thawed and reactivated during three consecutive prefermentations before the water kefir production process was started. Freezing and thawing damaged the water kefir grains irreversibly, as their structure did not restore during the prefermentations nor the production process...
January 9, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28041812/metabolic-adaptation-via-regulated-enzyme-degradation-in-the-pathogenic-yeast-candida-albicans
#6
S Y Ting, O A Ishola, M A Ahmed, Y M Tabana, S Dahham, M T Agha, S F Musa, R Muhammed, L T L Than, D Sandai
The virulence of Candida albicans is dependent upon fitness attributes as well as virulence factors. These attributes include robust stress responses and metabolic flexibility. The assimilation of carbon sources is important for growth and essential for the establishment of infections by C. albicans. Previous studies showed that the C. albicans ICL1 genes, which encode the glyoxylate cycle enzymes isocitratelyase are required for growth on non-fermentable carbon sources such as lactate and oleic acid and were repressed by 2% glucose...
December 29, 2016: Journal de Mycologie Médicale
https://www.readbyqxmd.com/read/28030573/cation-stress-responsive-transcription-factors-slta-and-crza-regulate-morphogenetic-processes-and-pathogenicity-of-colletotrichum-gloeosporioides
#7
Amit K Dubey, Shiri Barad, Neta Luria, Dilip Kumar, Eduardo A Espeso, Dov B Prusky
Growth of Colletotrichum gloeosporioides in the presence of cation salts NaCl and KCl inhibited fungal growth and anthracnose symptom of colonization. Previous reports indicate that adaptation of Aspergillus nidulans to salt- and osmotic-stress conditions revealed the role of zinc-finger transcription factors SltA and CrzA in cation homeostasis. Homologs of A. nidulans SltA and CrzA were identified in C. gloeosporioides. The C. gloeosporioides CrzA homolog is a 682-amino acid protein, which contains a C2H2 zinc finger DNA-binding domain that is highly conserved among CrzA proteins from yeast and filamentous fungi...
2016: PloS One
https://www.readbyqxmd.com/read/28027901/fungicide-bac8c-triggers-attenuation-of-mitochondrial-homeostasis-and-caspase-dependent-apoptotic-death
#8
Heejeong Lee, Dong Gun Lee
Bac8c (RIWVIWRR-NH2), an 8-mer peptide modified from amino acids 4-11 of Bac2a, shows broad-spectrum activity against pathogenic bacteria and yeast, and it has been the focus of attention owing to its low cost of synthesis. Although Bac8c is effective against Candida albicans, its mode of action needs to be investigated further. Bac8c causes yeast cell death in a dose-dependent manner by eliciting the production of reactive oxygen species, thereby attenuating the antioxidant defense system. It is also involved in Ca(2+) signaling, and produces apoptotic features, such as phosphatidylserine externalization and DNA fragmentation...
December 24, 2016: Biochimie
https://www.readbyqxmd.com/read/28013188/a-capture-method-based-on-the-vc1-domain-reveals-new-binding-properties-of-the-human-receptor-for-advanced-glycation-end-products-rage
#9
Genny Degani, Alessandra A Altomare, Mara Colzani, Caterina Martino, Angelica Mazzolari, Guenter Fritz, Giulio Vistoli, Laura Popolo, Giancarlo Aldini
The Advanced Glycation and Lipoxidation End products (AGEs and ALEs) are a heterogeneous class of compounds derived from the non-enzymatic glycation or protein adduction by lipoxidation break-down products. The receptor for AGEs (RAGE) is involved in the progression of chronic diseases based on persistent inflammatory state and oxidative stress. RAGE is a pattern recognition receptor (PRR) and the inhibition of the interaction with its ligands or of the ligand accumulation have a potential therapeutic effect...
December 18, 2016: Redox Biology
https://www.readbyqxmd.com/read/27999589/empirical-validation-of-a-hypothesis-of-the-hormetic-selective-forces-driving-the-evolution-of-longevity-regulation-mechanisms
#10
Alejandra Gomez-Perez, Pavlo Kyryakov, Michelle T Burstein, Nimara Asbah, Forough Noohi, Tania Iouk, Vladimir I Titorenko
Exogenously added lithocholic bile acid and some other bile acids slow down yeast chronological aging by eliciting a hormetic stress response and altering mitochondrial functionality. Unlike animals, yeast cells do not synthesize bile acids. We therefore hypothesized that bile acids released into an ecosystem by animals may act as interspecies chemical signals that generate selective pressure for the evolution of longevity regulation mechanisms in yeast within this ecosystem. To empirically verify our hypothesis, in this study we carried out a three-step process for the selection of long-lived yeast species by a long-term exposure to exogenous lithocholic bile acid...
2016: Frontiers in Genetics
https://www.readbyqxmd.com/read/27992471/overexpression-of-poplar-pyrabactin-resistance-like-abscisic-acid-receptors-promotes-abscisic-acid-sensitivity-and-drought-resistance-in-transgenic-arabidopsis
#11
Jingling Yu, Lei Yang, Xiaobing Liu, Renjie Tang, Yuan Wang, Haiman Ge, Mengting Wu, Jiang Zhang, Fugeng Zhao, Sheng Luan, Wenzhi Lan
Drought stress is an important environmental factor limiting productivity of plants, especially fast growing species with high water consumption like poplar. Abscisic acid (ABA) is a phytohormone that positively regulates seed dormancy and drought resistance. The PYR1 (Pyrabactin Resistance 1)/ PYRL (PYR-Like)/ RCAR (Regulatory Component of ABA Receptor) (PYR/PYL/RCAR) ABA receptor family has been identified and widely characterized in Arabidopsis thaliana. However, their functions in poplars remain unknown...
2016: PloS One
https://www.readbyqxmd.com/read/27984092/the-budding-yeast-orthologue-of-parkinson-s-disease-associated-dj-1-is-a-multi-stress-response-protein-protecting-cells-against-toxic-glycolytic-products
#12
Urszula Natkańska, Adrianna Skoneczna, Marzena Sieńko, Marek Skoneczny
Saccharomyces cerevisiae Hsp31p is a DJ-1/ThiJ/PfpI family protein that was previously shown to be important for survival in the stationary phase of growth and under oxidative stress. Recently, it was identified as a chaperone or as glutathione-independent glyoxalase. To elucidate the role played by this protein in budding yeast cells, we investigated its involvement in the protection against diverse environmental stresses. Our study revealed that HSP31 gene expression is controlled by multiple transcription factors, including Yap1p, Cad1p, Msn2p, Msn4p, Haa1p and Hsf1p...
October 27, 2016: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/27966978/quantitative-global-proteomics-of-yeast-pbp1-deletion-mutants-and-their-stress-responses-identifies-glucose-metabolism-mitochondrial-and-stress-granule-changes
#13
Gunnar Seidel, David Meierhofer, Nesli-Ece Şen, Anika Guenther, Sylvia Krobitsch, Georg Auburger
The yeast protein PBP1 is implicated in very diverse pathways. Intriguingly, its deletion mitigates the toxicity of human neurodegeneration factors. Here, we performed label-free quantitative global proteomics to identify crucial downstream factors, either without stress or under cell stress conditions (heat and NaN3). Compared to the wildtype BY4741 strain, PBP1 deletion always triggered downregulation of the key bioenergetics enzyme KGD2 and the prion protein RNQ1 as well as upregulation of the leucine biosynthesis enzyme LEU1...
December 22, 2016: Journal of Proteome Research
https://www.readbyqxmd.com/read/27957393/stress-responses-of-the-oil-producing-green-microalga-botryococcus-braunii-race-b
#14
Ivette Cornejo-Corona, Hem R Thapa, Daniel R Browne, Timothy P Devarenne, Edmundo Lozoya-Gloria
Plants react to biotic and abiotic stresses with a variety of responses including the production of reactive oxygen species (ROS), which may result in programmed cell death (PCD). The mechanisms underlying ROS production and PCD have not been well studied in microalgae. Here, we analyzed ROS accumulation, biomass accumulation, and hydrocarbon production in the colony-forming green microalga Botryococcus braunii in response to several stress inducers such as NaCl, NaHCO3, salicylic acid (SA), methyl jasmonate, and acetic acid...
2016: PeerJ
https://www.readbyqxmd.com/read/27941652/functional-divergence-of-poplar-histidine-aspartate-kinase-hk1-paralogs-in-response-to-osmotic-stress
#15
François Héricourt, Françoise Chefdor, Inès Djeghdir, Mélanie Larcher, Florent Lafontaine, Vincent Courdavault, Daniel Auguin, Franck Coste, Christiane Depierreux, Mirai Tanigawa, Tatsuya Maeda, Gaëlle Glévarec, Sabine Carpin
Previous works have shown the existence of protein partnerships belonging to a MultiStep Phosphorelay (MSP) in Populus putatively involved in osmosensing. This study is focused on the identification of a histidine-aspartate kinase, HK1b, paralog of HK1a. The characterization of HK1b showed its ability to homo- and hetero-dimerize and to interact with a few Histidine-containing Phosphotransfer (HPt) proteins, suggesting a preferential partnership in poplar MSP linked to drought perception. Furthermore, determinants for interaction specificity between HK1a/1b and HPts were studied by mutagenesis analysis, identifying amino acids involved in this specificity...
December 8, 2016: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/27940268/transcription-activation-activity-of-erd15-protein-from-morus-indica
#16
Bushra Saeed, Paramjit Khurana
Early Responsive to Dehydration (ERD) genes are described as rapid response mediators of dehydration stress. Recently, ERD15 has emerged as a novel stress induced transcription factor which might be involved in mediating distinct stress responses in plants. In order to determine whether mulberry ERD15 can act as functional transcription factor, yeast-based assays were performed. Mulberry ERD15 was found to drive high level reporter gene expression in yeast which suggests it may function as a transcription factor...
November 27, 2016: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/27936160/a-cbl-interacting-protein-kinase-tacipk2-confers-drought-tolerance-in-transgenic-tobacco-plants-through-regulating-the-stomatal-movement
#17
Yan Wang, Tao Sun, Tingting Li, Meng Wang, Guangxiao Yang, Guangyuan He
In plants, the CBL-CIPK signaling pathways play key roles in the response to abiotic stresses. However, functional studies of CIPKs in the important staple crop wheat are very rare. In this study, we identified a CIPK gene from wheat, designated TaCIPK2. Expression analysis results showed that TaCIPK2 could be up-regulated in wheat leaves by polyethylene glycol, abscisic acid and H2O2 treatments. Subcellular localization analyses revealed that TaCIPK2 was present in whole wheat epidermal cells. A yeast two-hybrid assay indicated that TaCIPK2 interacted with TaCBL1, 2, 3 and 4 in vitro...
2016: PloS One
https://www.readbyqxmd.com/read/27933076/overexpression-of-tanac2d-displays-opposite-responses-to-abiotic-stresses-between-seedling-and-mature-stage-of-transgenic-arabidopsis
#18
Quanjun Huang, Yan Wang
Environmental stresses frequently affect plant growth and development, and many genes have been found to be induced by unfavorable environmental conditions. Here, we reported the biological functions of TaNAC2D, a stress-related NAC (NAM, ATAF, and CUC) gene from wheat. TaNAC2D showed transcriptional activator activity in yeast. TaNAC2D-GFP fusion protein was localized in the nucleus of wheat mesophyll protoplasts. TaNAC2D transcript abundance was significantly induced by NaCl, PEG6000, and abscisic acid (ABA) at seedling stage, and repressed by NaCl and PEG6000 at mature plant stage...
2016: Frontiers in Plant Science
https://www.readbyqxmd.com/read/27933075/a-non-specific-setaria-italica-lipid-transfer-protein-gene-plays-a-critical-role-under-abiotic-stress
#19
Yanlin Pan, Jianrui Li, Licong Jiao, Cong Li, Dengyun Zhu, Jingjuan Yu
Lipid transfer proteins (LTPs) are a class of cysteine-rich soluble proteins having small molecular weights. LTPs participate in flower and seed development, cuticular wax deposition, also play important roles in pathogen and abiotic stress responses. A non-specific LTP gene (SiLTP) was isolated from a foxtail millet (Setaria italica) suppression subtractive hybridization library enriched for differentially expressed genes after abiotic stress treatments. A semi-quantitative reverse transcriptase PCR analysis showed that SiLTP was expressed in all foxtail millet tissues...
2016: Frontiers in Plant Science
https://www.readbyqxmd.com/read/27931189/adaptation-of-propionibacterium-freudenreichii-to-long-term-survival-under-gradual-nutritional-shortage
#20
Flavia Figueira Aburjaile, Marine Rohmer, Hugues Parrinello, Marie-Bernadette Maillard, Eric Beaucher, Gwénaële Henry, Aurélie Nicolas, Marie-Noëlle Madec, Anne Thierry, Sandrine Parayre, Stéphanie-Marie Deutsch, Muriel Cocaign-Bousquet, Anderson Miyoshi, Vasco Azevedo, Yves Le Loir, Hélène Falentin
BACKGROUND: Propionibacterium freudenreichii is an Actinobacterium widely used in the dairy industry as a ripening culture for Swiss-type cheeses, for vitamin B12 production and some strains display probiotic properties. It is reportedly a hardy bacterium, able to survive the cheese-making process and digestive stresses. RESULTS: During this study, P. freudenreichii CIRM-BIA 138 (alias ITG P9), which has a generation time of five hours in Yeast Extract Lactate medium at 30 °C under microaerophilic conditions, was incubated for 11 days (9 days after entry into stationary phase) in a culture medium, without any adjunct during the incubation...
December 8, 2016: BMC Genomics
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