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Acid stress yeast

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https://www.readbyqxmd.com/read/28521568/prion-based-memory-of-heat-stress-in-yeast
#1
Tatiana A Chernova, Yury O Chernoff, Keith D Wilkinson
Amyloids and amyloid-based prions are self-perpetuating protein aggregates which can spread by converting a normal protein of the same sequence into a prion form. They are associated with diseases in humans and mammals, and control heritable traits in yeast and other fungi. Some amyloids are implicated in biologically beneficial processes. As prion formation generates reproducible memory of a conformational change, prions can be considered as molecular memory devices.  We have demonstrated that in yeast, stress-inducible cytoskeleton-associated protein Lsb2 forms a metastable prion in response to high temperature...
May 19, 2017: Prion
https://www.readbyqxmd.com/read/28503907/proteomics-analysis-for-asymmetric-inheritance-of-preexisting-proteins-between-mother-and-daughter-cells-in-budding-yeast
#2
Mitsuhiro Okada, Shunta Kusunoki, Yuko Ishibashi, Keiji Kito
In budding yeast, a mother cell can produce a finite number of daughter cells over its life. The accumulation of a variety of types of damaged components has an impact on the aging process. Asymmetrical inheritance during cell division causes these aberrant intracellular constituents to be retained in mother cells and prevents them from segregating to daughter cells. However, the understanding of asymmetrical inheritance of individual proteins that are damaged or old age, and their relevance to the aging process, has been limited...
May 15, 2017: Genes to Cells: Devoted to Molecular & Cellular Mechanisms
https://www.readbyqxmd.com/read/28497532/arabidopsis-thaliana-epoxide-hydrolase1-ateh1-is-a-cytosolic-epoxide-hydrolase-involved-in-the-synthesis-of-poly-hydroxylated-cutin-monomers
#3
Emmanuelle Pineau, Lin Xu, Hugues Renault, Adrien Trolet, Nicolas Navrot, Pascaline Ullmann, Bertrand Légeret, Gaëtan Verdier, Fred Beisson, Franck Pinot
Epoxide hydrolases (EHs) are present in all living organisms. They have been extensively characterized in mammals; however, their biological functions in plants have not been demonstrated. Based on in silico analysis, we identified AtEH1 (At3g05600), a putative Arabidopsis thaliana epoxide hydrolase possibly involved in cutin monomer synthesis. We expressed AtEH1 in yeast and studied its localization in vivo. We also analyzed the composition of cutin from A. thaliana lines in which this gene was knocked out...
May 12, 2017: New Phytologist
https://www.readbyqxmd.com/read/28488916/observing-the-fate-of-trna-and-its-modifications-by-nucleic-acid-isotope-labeling-mass-spectrometry-nail-ms
#4
Matthias Heiss, Valentin F Reichle, Stefanie Kellner
RNA in yeast, especially rRNA and tRNA are heavily modified to fulfill their function in protein translation. Using biosynthetic stable isotope labeled internal standards we quantified 12 modified nucleosides in tRNA from S. cerevisiae over 24 hours. We observed different quantities of modified nucleosides in dependence of the growth phase. To elucidate the underlying mechanism of the observed tRNA epitranscriptome adaptation, it is necessary to distinguish the pre-existing tRNA pool and its modifications from newly-synthesized tRNAs...
May 10, 2017: RNA Biology
https://www.readbyqxmd.com/read/28487715/overexpression-of-a-tartary-buckwheat-gene-ftbhlh3-enhances-drought-oxidative-stress-tolerance-in-transgenic-arabidopsis
#5
Pan-Feng Yao, Cheng-Lei Li, Xue-Rong Zhao, Mao-Fei Li, Hai-Xia Zhao, Jin-Ya Guo, Yi Cai, Hui Chen, Qi Wu
bHLH (basic helix-loop-helix) transcription factors play important roles in the abiotic stress response in plants, but their characteristics and functions in tartary buckwheat (Fagopyrum tataricum), a flavonoid-rich cereal crop with a strong stress tolerance, have not been fully investigated. Here, a novel bHLH gene, designated FtbHLH3, was isolated and characterized. Expression analysis in tartary buckwheat revealed that FtbHLH3 was mainly induced by polyethylene glycol 6000 (PEG6000) and abscisic acid (ABA) treatments...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28484514/increased-lignocellulosic-inhibitor-tolerance-of-saccharomyces-cerevisiae-cell-populations-in-early-stationary-phase
#6
Venkatachalam Narayanan, Jenny Schelin, Marie Gorwa-Grauslund, Ed Wj van Niel, Magnus Carlquist
BACKGROUND: Production of second-generation bioethanol and other bulk chemicals by yeast fermentation requires cells that tolerate inhibitory lignocellulosic compounds at low pH. Saccharomyces cerevisiae displays high plasticity with regard to inhibitor tolerance, and adaptation of cell populations to process conditions is essential for reaching efficient and robust fermentations. RESULTS: In this study, we assessed responses of isogenic yeast cell populations in different physiological states to combinations of acetic acid, vanillin and furfural at low pH...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28482005/the-rapid-evolution-of-an-ohnolog-contributes-to-the-ecological-specialization-of-incipient-yeast-species
#7
Chris Eberlein, Lou Nielly-Thibault, Halim Maaroufi, Alexandre K Dubé, Jean-Baptiste Leducq, Guillaume Charron, Christian R Landry
Identifying the molecular changes that lead to ecological specialization during speciation is one of the major goals of molecular evolution. One question that remains to be thoroughly investigated is whether ecological specialization derives strictly from adaptive changes and their associated trade-offs, or from conditionally neutral mutations that accumulate under relaxed selection. We used whole-genome sequencing, genome annotation and computational analyses to identify genes that have rapidly diverged between two incipient species of Saccharomyces paradoxus that occupy different climatic regions along a south-west to north-east gradient...
May 8, 2017: Molecular Biology and Evolution
https://www.readbyqxmd.com/read/28476241/evaluation-of-saccharomyces-cerevisiae-gas1-with-respect-to-its-involvement-in-tolerance-to-low-ph-and-salt-stress
#8
Akinori Matsushika, Toshihiro Suzuki, Tetsuya Goshima, Tamotsu Hoshino
We previously showed that overexpression of IoGAS1, which was isolated from the multiple stress-tolerant yeast Issatchenkia orientalis, endows Saccharomyces cerevisiae cells with the ability to grow and ferment under acidic and high-salt conditions. The deduced amino acid sequence of the IoGAS1 gene product exhibits 60% identity with the S. cerevisiae Gas1 protein, a glycosylphosphatidylinositol-anchored protein essential for maintaining cell wall integrity. However, the functional roles of ScGAS1 in stress tolerance and pH regulation remain unclear...
May 2, 2017: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/28470798/alternative-pathways-for-escherichia-coli-biofilm-formation-revealed-by-srna-overproduction
#9
Ashley Parker, Suanur Cureoglu, Nicholas De Lay, Nadim Majdalani, Susan Gottesman
Small regulatory RNAs have major roles in many regulatory circuits in Escherichia coli and other bacteria, including the transition from planktonic to biofilm growth. We tested Hfq-dependent sRNAs in E. coli for their ability, when overproduced, to inhibit or stimulate biofilm formation, in two different growth media. We identify two mutually exclusive pathways for biofilm formation. In LB, PgaA, encoding an adhesion export protein, played a critical role; biofilm was independent of the general stress factor RpoS or CsgD, regulator of curli and other biofilm genes...
May 4, 2017: Molecular Microbiology
https://www.readbyqxmd.com/read/28460089/genome-sequence-of-the-highly-weak-acid-tolerant-zygosaccharomyces-bailii-ist302-amenable-to-genetic-manipulations-and-physiological-studies
#10
Margarida Palma, Martin Münsterkötter, João Peça, Ulrich Güldener, Isabel Sá-Correia
Zygosaccharomyces bailii is one of the most problematic spoilage yeast species found in the food and beverage industry particularly in acidic products, due to its exceptional resistance to weak acid stress. This article describes the annotation of the genome sequence of Z. bailii IST302, a strain recently proven to be amenable to genetic manipulations and physiological studies. The work was based on the annotated genomes of strain ISA1307, an interspecies hybrid between Z. bailii and a closely related species, and the Z...
April 29, 2017: FEMS Yeast Research
https://www.readbyqxmd.com/read/28452060/cimyb17-a-stress-induced-chicory-r2r3-myb-transcription-factor-activates-promoters-of-genes-involved-in-fructan-synthesis-and-degradation
#11
Hongbin Wei, Anja Bausewein, Steffen Greiner, Nicolas Dauchot, Karsten Harms, Thomas Rausch
In Cichorium intybus, inulin metabolism is mediated by fructan-active enzymes (FAZYs): sucrose:sucrose 1-fructosyltransferase (1-SST), fructan:fructan 1-fructosyltransferase (1-FFT), and fructan 1-exohydrolases 1, 2a and 2b (1-FEH1, -2a and -2b), respectively. While these enzymes have been rigorously characterized, the transcriptional network orchestrating their development- and stress-related expression has remained largely unknown. Here, the possible role of R2R3-MYB transcription factors in FAZY regulation was explored via bioinformatic identification of R2R3-MYBs (using an RNA sequencing (RNAseq) database), studies of co-expression of these factors with target genes, in vivo transient transactivation assays of FAZY target promoters (dual luciferase assay), and a yeast one-hybrid assay investigating the specificity of the binding of these factors to cis-elements...
April 28, 2017: New Phytologist
https://www.readbyqxmd.com/read/28450371/adaptive-roles-of-ssy1-and-sir3-during-cycles-of-growth-and-starvation-in-saccharomyces-cerevisiae-populations-enriched-for-quiescent-or-non-quiescent-cells
#12
Dominika M Wloch-Salamon, Katarzyna Tomala, Dimitra Aggeli, Barbara Dunn
Over its evolutionary history, Saccharomyces cerevisiae has evolved to be well-adapted to fluctuating nutrient availability. In the presence of sufficient nutrients, yeast cells continue to proliferate, but upon starvation haploid yeast cells enter stationary phase and differentiate into non-quiescent (NQ) and quiescent (Q) cells. Q cells survive stress better than NQ cells and show greater viability when nutrient-rich conditions are restored. To investigate the genes that may be involved in the differentiation of Q and NQ cells we serially propagated yeast populations that were enriched for either only Q or only NQ cell types over many repeated growth-starvation cycles...
April 26, 2017: G3: Genes—Genomes—Genetics
https://www.readbyqxmd.com/read/28431563/inheritance-of-brewing-relevant-phenotypes-in-constructed-saccharomyces-cerevisiae%C3%A2-%C3%A3-%C3%A2-saccharomyces-eubayanus-hybrids
#13
Kristoffer Krogerus, Tuulikki Seppänen-Laakso, Sandra Castillo, Brian Gibson
BACKGROUND: Interspecific hybridization has proven to be a potentially valuable technique for generating de novo lager yeast strains that possess diverse and improved traits compared to their parent strains. To further enhance the value of hybridization for strain development, it would be desirable to combine phenotypic traits from more than two parent strains, as well as remove unwanted traits from hybrids. One such trait, that has limited the industrial use of de novo lager yeast hybrids, is their inherent tendency to produce phenolic off-flavours; an undesirable trait inherited from the Saccharomyces eubayanus parent...
April 21, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28428821/genome-wide-search-for-candidate-genes-for-yeast-robustness-improvement-against-formic-acid-reveals-novel-susceptibility-trk1-and-positive-regulators-and-resistance-haa1-regulon-determinants
#14
Sílvia F Henriques, Nuno P Mira, Isabel Sá-Correia
BACKGROUND: Formic acid is an inhibitory compound present in lignocellulosic hydrolysates. Understanding the complex molecular mechanisms underlying Saccharomyces cerevisiae tolerance to this weak acid at the system level is instrumental to guide synthetic pathway engineering for robustness improvement of industrial strains envisaging their use in lignocellulosic biorefineries. RESULTS: This study was performed to identify, at a genome-wide scale, genes whose expression confers protection or susceptibility to formic acid, based on the screening of a haploid deletion mutant collection to search for these phenotypes in the presence of 60, 70 and 80 mM of this acid, at pH 4...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28419348/litchi-fruit-lcnac1-is-a-target-of-lcmyc2-and-regulator-of-fruit-senescence-through-its-interaction-with-lcwrky1
#15
Guoxiang Jiang, Huiling Yan, Fuwang Wu, Dandan Zhang, Wei Zeng, Hongxia Qu, Feng Chen, Li Tan, Xuewu Duan, Yueming Jiang
Senescence is a key factor resulting in deterioration of non-climacteric fruit. NAC transcription factors are important regulators in plant development and abiotic stress responses, yet little information regarding the role of NACs in regulating non-climacteric fruit senescence is available. In this study, we cloned 13 NAC genes from litchi (Litchi chinensis) fruit, and analyzed subcellular localization and expression profiles of these genes during postharvest natural and low-temperature-delayed senescence...
April 13, 2017: Plant & Cell Physiology
https://www.readbyqxmd.com/read/28411489/kale-borack1-is-involved-in-the-plant-response-to-salt-stress-and-peronospora-brassicae-gaumann
#16
Da-Hong Li, Fu-Jia Shen, Hong-Yan Li, Wei Li
The receptor for activated C kinase 1 (RACK1) belongs to a protein subfamily containing a tryptophan-aspartic acid-domain (WD) repeat structure. Compelling evidence indicates that RACK1 can interact with many signal molecules and affect different signal transduction pathways. In this study, a kale (Brassica oleracea var. acephala f.tricolor) RACK1 gene (BoRACK1) was cloned by RT-PCR. The amino acid sequence of BoRACK1 had seven WD repeats in which there were typical GH (glycine-histidine) and WD dipeptides...
March 24, 2017: Journal of Plant Physiology
https://www.readbyqxmd.com/read/28409382/yeast-response-and-tolerance-to-benzoic-acid-involves-the-gcn4-and-stp1-regulated-multidrug-multixenobiotic-resistance-transporter-tpo1
#17
Cláudia P Godinho, Nuno P Mira, Tânia R Cabrito, Miguel C Teixeira, Kaur Alasoo, Joana F Guerreiro, Isabel Sá-Correia
The action of benzoic acid in the food and beverage industries is compromised by the ability of spoilage yeasts to cope with this food preservative. Benzoic acid occurs naturally in many plants and is an intermediate compound in the biosynthesis of many secondary metabolites. The understanding of the mechanisms underlying the response and resistance to benzoic acid stress in the eukaryotic model yeast is thus crucial to design more suitable strategies to deal with this toxic lipophilic weak acid. In this study, the Saccharomyces cerevisiae multidrug transporter Tpo1 was demonstrated to confer resistance to benzoic acid...
April 13, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28405910/omics-analysis-of-acetic-acid-tolerance-in-saccharomyces-cerevisiae
#18
REVIEW
Peng Geng, Liang Zhang, Gui Yang Shi
Acetic acid is an inhibitor in industrial processes such as wine making and bioethanol production from cellulosic hydrolysate. It causes energy depletion, inhibition of metabolic enzyme activity, growth arrest and ethanol productivity losses in Saccharomyces cerevisiae. Therefore, understanding the mechanisms of the yeast responses to acetic acid stress is essential for improving acetic acid tolerance and ethanol production. Although 329 genes associated with acetic acid tolerance have been identified in the Saccharomyces genome and included in the database ( http://www...
May 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28398122/investigating-proteome-and-transcriptome-defense-response-of-apples-induced-by-yarrowia-lipolytica
#19
Hongyin Zhang, Liangliang Chen, Yiwen Sun, Lina Zhao, Xiangfeng Zheng, Qiya Yang, Xiaoyun Zhang
A better understanding of the mode of action of postharvest biocontrol agents on fruit surfaces is critical for the advancement of successful implementation of postharvest biocontrol products. This is due to the increasing importance of biological control of postharvest diseases over chemical and other control methods. However, most of the mechanisms involved in biological control remain unknown and need to be explored. Yarrowia lipolytica significantly inhibited blue mold decay of apples caused by Penicillium expansum...
April 2017: Molecular Plant-microbe Interactions: MPMI
https://www.readbyqxmd.com/read/28392791/progress-toward-understanding-protein-s-acylation-prospective-in-plants
#20
REVIEW
Yaxiao Li, Baoxiu Qi
S-acylation, also known as S-palmitoylation or palmitoylation, is a reversible post-translational lipid modification in which long chain fatty acid, usually the 16-carbon palmitate, covalently attaches to a cysteine residue(s) throughout the protein via a thioester bond. It is involved in an array of important biological processes during growth and development, reproduction and stress responses in plant. S-acylation is a ubiquitous mechanism in eukaryotes catalyzed by a family of enzymes called Protein S-Acyl Transferases (PATs)...
2017: Frontiers in Plant Science
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