keyword
MENU ▼
Read by QxMD icon Read
search

Acid stress yeast

keyword
https://www.readbyqxmd.com/read/29140457/genistein-specific-g6dt-gene-for-the-inducible-production-of-wighteone-in-lotus-japonicus
#1
Jinyue Liu, Wenbo Jiang, Yaying Xia, Xuemin Wang, Guoan Shen, Yongzhen Pang
Prenylated isoflavonoids were found in several legume plants, and they possess various biological activities that play important roles in both plant defense and human health. However, it is still unknown whether prenylated isoflavonoids are present in the model legume plant L. japonicus. In the present study, we found that the prenylated isoflavonoid wighteone was produced in L. japonicus when the leaf was supplemented with genistein. Furthermore, a novel prenyltransferase gene LjG6DT was identified, which shared high similarity and was closely related with several known prenyltransferase genes involved in isoflavonoid biosynthesis...
November 10, 2017: Plant & Cell Physiology
https://www.readbyqxmd.com/read/29124795/proteomic-analysis-of-lysine-acetylation-provides-strong-evidence-for-acetylated-proteins-involved-in-plant-meiosis-and-tapetum-function
#2
Xiaojing Li, Juanying Ye, Hong Ma, Pingli Lu
Protein lysine acetylation (KAC) is a dynamic and reversible post-translational modification, playing important biological roles in many organisms. Although KAC was shown to affect reproductive development and meiosis in yeast and animals, similar studies are largely lacking in flowering plants, especially in a proteome-scale investigation for a certain reproductive stage. Here, we reported results from a proteomic investigation to detect KAC status of the developing rice anthers near the time of meiosis (RAM), providing strong biochemical evidence for roles of many KAC-affected proteins during rice anther development and meiosis...
November 10, 2017: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/29116578/yeast-polyubiquitin-gene-ubi4-deficiency-leads-to-early-induction-of-apoptosis-and-shortened-replicative-lifespan
#3
Wei Zhao, Tao Zhou, Hua-Zhen Zheng, Kun-Pei Qiu, Hong-Jing Cui, Hui Yu, Xin-Guang Liu
Ubiquitin is a 76-amino acid protein that is highly conserved among higher and lower eukaryotes. The polyubiquitin gene UBI4 encodes a unique precursor protein that contains five ubiquitin repeats organized in a head-to-tail arrangement. Although the involvement of the yeast polyubiquitin gene UBI4 in the stress response was reported long ago, there are no reports regarding the underlying mechanism of this involvement. In this study, we used UBI4-deletion and UBI4-overexpressing yeast strains as models to explore the potential mechanism by which UBI4 protects yeast cells against paraquat-induced oxidative stress...
November 7, 2017: Cell Stress & Chaperones
https://www.readbyqxmd.com/read/29101531/investigating-the-underlying-mechanism-of-saccharomyces-cerevisiae-in-response-to-ethanol-stress-employing-rna-seq-analysis
#4
Ruoyun Li, Guotong Xiong, Shukun Yuan, Zufang Wu, Yingjie Miao, Peifang Weng
Saccharomyces cerevisiae has been widely used for wine fermentation and bio-fuels production. A S. cerevisiae strain Sc131 isolated from tropical fruit shows good fermentation properties and ethanol tolerance, exhibiting significant potential in Chinese bayberry wine fermentation. In this study, RNA-sequence and RT-qPCR was used to investigate the transcriptome profile of Sc131 in response to ethanol stress. Scanning Electron Microscopy were carried out to observe surface morphology of yeast cells. Totally, 937 genes were identified differential expressed, including 587 up-regulated and 350 down-regulated genes, after 4-h ethanol stress (10% v/v)...
November 3, 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/29101483/resveratrol-suppresses-ethanol-stress-in-winery-and-bottom-brewery-yeast-by-affecting-superoxide-dismutase-lipid-peroxidation-and-fatty-acid-profile
#5
Lucia Gharwalova, Karel Sigler, Jana Dolezalova, Jan Masak, Tomas Rezanka, Irena Kolouchova
Mid-exponential cultures of two traditional biotechnological yeast species, winery Saccharomyces cerevisiae and the less ethanol tolerant bottom-fermenting brewery Saccharomyces pastorianus, were exposed to different concentrations of added ethanol (3, 5 and 8%) The degree of ethanol-induced cell stress was assessed by measuring the cellular activity of superoxide dismutase (SOD), level of lipid peroxidation products, changes in cell lipid content and fatty acid profile. The resveratrol as an antioxidant was found to decrease the ethanol-induced rise of SOD activity and suppress the ethanol-induced decrease in cell lipids...
November 3, 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/29097398/cell-biology-of-the-mitochondrion
#6
Alexander M van der Bliek, Margaret M Sedensky, Phil G Morgan
Mitochondria are best known for harboring pathways involved in ATP synthesis through the tricarboxylic acid cycle and oxidative phosphorylation. Major advances in understanding these roles were made with Caenorhabditiselegans mutants affecting key components of the metabolic pathways. These mutants have not only helped elucidate some of the intricacies of metabolism pathways, but they have also served as jumping off points for pharmacology, toxicology, and aging studies. The field of mitochondria research has also undergone a renaissance, with the increased appreciation of the role of mitochondria in cell processes other than energy production...
November 2017: Genetics
https://www.readbyqxmd.com/read/29061122/metabolic-differentiation-of-surface-and-invasive-cells-of-yeast-colony-biofilms-revealed-by-gene-expression-profiling
#7
Jana Maršíková, Derek Wilkinson, Otakar Hlaváček, Gregor D Gilfillan, Alexandru Mizeranschi, Timothy Hughes, Markéta Begany, Stanislava Rešetárová, Libuše Váchová, Zdena Palková
BACKGROUND: Yeast infections are often connected with formation of biofilms that are extremely difficult to eradicate. An excellent model system for deciphering multifactorial determinants of yeast biofilm development is the colony biofilm, composed of surface ("aerial") and invasive ("root") cells. While surface cells have been partially analyzed before, we know little about invasive root cells. In particular, information on the metabolic, chemical and morphogenetic properties of invasive versus surface cells is lacking...
October 23, 2017: BMC Genomics
https://www.readbyqxmd.com/read/29050406/microbiological-quality-assessment-and-validation-of-antimicrobials-against-unstressed-or-cold-stress-adapted-salmonella-and-surrogate-enterococcus-faecium-on-broiler-carcasses-and-wings
#8
Lacey Lemonakis, KaWang Li, Jeremy M Adler, Cangliang Shen
This study aims to evaluate the microbiological quality and efficacy of antimicrobials to inactivate unstressed or cold-stress adapted Salmonella and Enterococcus on broiler carcasses and wings processed at a small USDA-inspected slaughter facility in West Virginia. The first part of the study included 42 carcasses that were pre- and secondarily-enriched in bacterial media followed by streak-plating onto XLT-4 and HardyCHROM™-agar Salmonella and confirmation using an API20E-kit. The aerobic plate counts (APC), Escherichia coli (ECC), total coliforms (TCC), and yeast/molds were analyzed on petri-films...
September 1, 2017: Poultry Science
https://www.readbyqxmd.com/read/29045490/the-ethylene-response-factor-aterf4-negatively-regulates-the-iron-deficiency-response-in-arabidopsis-thaliana
#9
Wei Liu, N J Umuhoza Karemera, Ting Wu, Yafei Yang, Xinzhong Zhang, Xuefeng Xu, Yi Wang, Zhenhai Han
Iron (Fe) deficiency is one of many conditions that can seriously damage crops. Low levels of photosynthesis can lead to the degradation of chlorophyll content and impaired respiration in affected plants, which together cause poor growth and reduce quality. Although ethylene plays an important role in responses to Fe deficiency, a limited number of studies have been carried out on ethylene response factor (ERFs) as components of plant regulation mechanisms. Thus, this study aimed to investigate the role of AtERF4 in plant responses to Fe deficiency...
2017: PloS One
https://www.readbyqxmd.com/read/29044827/identifying-the-mechanism-of-escherichia-coli-o157-h7-survival-by-the-addition-of-salt-in-the-treatment-with-organic-acids
#10
Young-Min Bae, Jae-Hyun Yoon, Ji-Yeon Kim, Sun-Young Lee
AIM: In this study, the effects of addition of salt to treatment with acids (one of several organic acids and salt in various solutions including rich or minimal broth, buffer, or distilled water) on the reduction of Escherichia coli O157:H7 were investigated. The protein expression profiles corresponding to acid stress (acetic acid) with or without salt addition were studied using a comparative proteomic analysis of E. coli O157:H7. METHODS AND RESULTS: When acetic, lactic, or propionic acid was combined with 3% NaCl, mutually antagonistic effects of acid and salt on viability of E...
October 17, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/29043484/the-role-of-yeast-m-6-a-methyltransferase-in-peroxisomal-fatty-acid-oxidation
#11
REVIEW
Pradeep Kumar Yadav, Praveen Kumar Rajvanshi, Ram Rajasekharan
The precise and controlled regulation of gene expression at transcriptional and post-transcriptional levels is crucial for the eukaryotic cell survival and functions. In eukaryotes, more than 100 types of post-transcriptional RNA modifications have been identified. The N(6)-methyladenosine (m(6)A) modification in mRNA is among the most common post-transcriptional RNA modifications known in eukaryotic organisms, and the m(6)A RNA modification can regulate gene expression. The role of yeast m(6)A methyltransferase (Ime4) in meiosis, sporulation, triacylglycerol metabolism, vacuolar morphology, and mitochondrial functions has been reported...
October 17, 2017: Current Genetics
https://www.readbyqxmd.com/read/29042608/sedum-alfredii-sanramp6-metal-transporter-contributes-to-cadmium-accumulation-in-transgenic-arabidopsis-thaliana
#12
Shuangshuang Chen, Xiaojiao Han, Jie Fang, Zhuchou Lu, Wenmin Qiu, Mingying Liu, Jian Sang, Jing Jiang, Renying Zhuo
The plant natural resistance-associated macrophage protein (Nramp) family plays an important role in tolerance to heavy metal stress. However, few Nramps have been functionally characterized in the heavy metal-accumulating plant Sedum alfredii. Here, Nramp6 was cloned and identified from S. alfredii and its function analyzed in transgenic Arabidopsis thaliana. SaNramp6 cDNA contains an open reading frame of 1, 638 bp encoding 545 amino acids. SaNramp6's expression can be induced by cadmium (Cd) stress, and, after treatment, it peaked at one week and 12 h in the roots and leaves, respectively...
October 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29040754/development-of-unique-cytotoxic-chimeric-antigen-receptors-based-on-human-scfv-targeting-b7h6
#13
Casey K Hua, Albert T Gacerez, Charles L Sentman, Margaret E Ackerman
As a stress-inducible natural killer (NK) cell ligand, B7H6 plays a role in innate tumor immunosurveillance and is a fairly tumor selective marker expressed on a variety of solid and hematologic cancer cells. Here, we describe the isolation and characterization of a new family of single chain fragment variable (scFv) molecules targeting the human B7H6 ligand. Through directed evolution of a yeast surface displayed non-immune human-derived scFv library, eight candidates comprising a single family of clones differing by up to four amino acid mutations and exhibiting nM avidities for soluble B7H6-Ig were isolated...
October 1, 2017: Protein Engineering, Design & Selection: PEDS
https://www.readbyqxmd.com/read/29036515/the-jasmonate-zim-domain-gene-family-mediates-ja-signaling-and-stress-response-in-cotton
#14
Heng Sun, Lin Chen, Jianying Li, Meiling Hu, Abid Ullah, Xin He, Xiyan Yang, Xianlong Zhang
JASMONATE ZIM-domain (JAZ) family proteins are involved in regulating diverse biological processes in plants. However, their functions have not been well characterized in cotton (Gossypium spp.). In the present study, 13, 15, 25, and 30 JAZ genes were identified in Gossypium arboretum, Gossypium raimondii, Gossypium barbadense and Gossypium hirsutum respectively, based on gene homology. Selection and variation analyses showed that the SNP density of GhJAZ genes in wild species was much higher than that in domesticated species...
October 3, 2017: Plant & Cell Physiology
https://www.readbyqxmd.com/read/29023415/characterization-function-and-transcriptional-profiling-analysis-of-3-hydroxy-3-methylglutaryl-coa-synthase-gene-gbhmgs1-towards-stresses-and-exogenous-hormone-treatments-in-ginkgo-biloba
#15
Xiangxiang Meng, Qiling Song, Jiabao Ye, Lanlan Wang, Feng Xu
3-Hydroxy-3-methylglutaryl-CoA synthase (HMGS) is one of the rate-limiting enzymes in the mevalonate pathway as it catalyzes the condensation of acetoacetyl-CoA to form 3-hydroxy-3-methylglutaryl-CoA. In this study, A HMGS gene (designated as GbHMGS1) was cloned from Ginkgo biloba for the first time. GbHMGS1 contained a 1422-bp open-reading frame encoding 474 amino acids. Comparative and bioinformatics analysis revealed that GbHMGS1 was extensively homologous to HMGSs from other plant species. Phylogenetic analysis indicated that the GbHMGS1 belonged to the plant HMGS superfamily, sharing a common evolutionary ancestor with other HMGSs, and had a further relationship with other gymnosperm species...
October 12, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28993899/industrial-antifoam-agents-impair-ethanol-fermentation-and-induce-stress-responses-in-yeast-cells
#16
Jens Christian Nielsen, Felipe Senne de Oliveira Lino, Thomas Gundelund Rasmussen, Jette Thykær, Christopher T Workman, Thiago Olitta Basso
The Brazilian sugarcane industry constitutes one of the biggest and most efficient ethanol production processes in the world. Brazilian ethanol production utilizes a unique process, which includes cell recycling, acid wash, and non-aseptic conditions. Process characteristics, such as extensive CO2 generation, poor quality of raw materials, and frequent contaminations, all lead to excessive foam formation during fermentations, which is treated with antifoam agents (AFA). In this study, we have investigated the impact of industrial AFA treatments on the physiology and transcriptome of the industrial ethanol strain Saccharomyces cerevisiae CAT-1...
October 10, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28988107/calcium-dependent-protein-kinase-21-phosphorylates-14-3-3-proteins-in-response-to-aba-signaling-and-salt-stress-in-rice
#17
Yixing Chen, Xiaojin Zhou, Shu Chang, Zhilin Chu, Hanmeng Wang, Shengcheng Han, Yingdian Wang
The calcium-dependent protein kinases (CDPKs) are a class of plant-specific kinase that directly bind Ca(2+) and mediate the calcium-signaling pathways to play important physiological roles in growth and development. The rice genome contains 31 CDPK genes, one of which, OsCPK21, is known to modulate the abscisic acid (ABA) and salt stress responses in this crop; however, the molecular mechanisms underlying this regulation are largely unknown. In the present study, we performed yeast two-hybrid screening, glutathione S-transferase pull-down, co-immunoprecipitation, and bimolecular fluorescence complementation assays to confirm the interaction between OsCPK21 and one of its putative targets, Os14-3-3 (OsGF14e)...
December 2, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28976247/investigation-of-the-effect-of-biologically-active-threo-ds-isocitric-acid-on-oxidative-stress-in-paramecium-caudatum
#18
Igor G Morgunov, Olga V Karpukhina, Svetlana V Kamzolova, Vladimir A Samoilenko, Anatoly N Inozemtsev
The effect of biologically active form (threo-Ds-) of isocitric acid on oxidative stress was studied using the infusorian Paramecium caudatum stressed by hydrogen peroxide and salts of some heavy metals (Cu, Pb, Zn, and Cd). Isocitric acid at concentrations between 0.5 and 10 mM favorably influenced the infusorian cells with oxidative stress induced by the toxicants studied. The maximal antioxidant effect of isocitric acid was observed at its concentration 10 mM irrespective of the toxicant used (either H2O2 or heavy metal ions)...
October 4, 2017: Preparative Biochemistry & Biotechnology
https://www.readbyqxmd.com/read/28971800/an-unfolded-protein-induced-conformational-switch-activates-mammalian-ire1
#19
Gülsün Elif Karagöz, Diego Acosta-Alvear, Hieu T Nguyen, Crystal P Lee, Feixia Chu, Peter Walter
The unfolded protein response (UPR) adjusts the cell's protein folding capacity in the endoplasmic reticulum (ER) according to need. IRE1 is the most conserved UPR sensor in eukaryotic cells. It has remained controversial, however, whether mammalian and yeast IRE1 use a common mechanism for ER stress sensing. Here, we show that similar to yeast, human IRE1α's ER-lumenal domain (hIRE1α LD) binds peptides with a characteristic amino acid bias. Peptides and unfolded proteins bind to hIRE1α LD's MHC-like groove and induce allosteric changes that lead to its oligomerization...
October 3, 2017: ELife
https://www.readbyqxmd.com/read/28955998/phospholipases-d%C3%AE-and-%C3%AE-are-involved-in-local-and-systemic-wound-responses-of-cotton-g-hirsutum
#20
Angeliki Bourtsala, Theodora Farmaki, Dia Galanopoulou
Phospholipases D (PLDs) catabolize structural phospholipids to produce phosphatidic acid (PtdOH), a lipid playing central role in signalling pathways in animal, yeast and plant cells. In animal cells two PLD genes have been studied while in model plant Arabidopsis twelve genes exist, classified in six classes (α-ζ). This underlines the role of these enzymes in plant responses to environmental stresses. However, information concerning the PLD involvement in the widely cultivated and economically important cotton plant responses is very limited...
March 2017: Biochemistry and Biophysics Reports
keyword
keyword
28594
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"