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https://www.readbyqxmd.com/read/28938619/anti-cancer-efficacy-of-biotinylated-chitosan-nanoparticles-in-liver-cancer
#1
Mingrong Cheng, Weiping Zhu, Qing Li, Dejian Dai, Yiming Hou
The present study investigated the synthesis of biotinylated chitosan (Bio-CS) from chitosan using a nanomaterial skeleton with biotin and the successful targeting of the formulation in liver cancer cells. Bio-CS was validated by fourier transformed infrared spectroscopy and hydrogen(-1) nuclear magnetic resonance spectroscopy. Bio-CS and plasmid DNA were used to construct Bio-CS/plasmid DNA nanoparticles according to the optimal molar ratio of 1:1 and the optimal pH-value of 5.5. Under these conditions, the parameters mean particle size, potential, encapsulation rate and drug loading, were 82...
August 29, 2017: Oncotarget
https://www.readbyqxmd.com/read/28938578/quantitative-dot-blot-analysis-qdb-a-versatile-high-throughput-immunoblot-method
#2
Geng Tian, Fangrong Tang, Chunhua Yang, Wenfeng Zhang, Jonas Bergquist, Bin Wang, Jia Mi, Jiandi Zhang
Lacking access to an affordable method of high throughput immunoblot analysis for daily use remains a big challenge for scientists worldwide. We proposed here Quantitative Dot Blot analysis (QDB) to meet this demand. With the defined linear range, QDB analysis fundamentally transforms traditional immunoblot method into a true quantitative assay. Its convenience in analyzing large number of samples also enables bench scientists to examine protein expression levels from multiple parameters. In addition, the small amount of sample lysates needed for analysis means significant saving in research sources and efforts...
August 29, 2017: Oncotarget
https://www.readbyqxmd.com/read/28938496/icaritin-improves-antibody-induced-thrombocytopenia-in-a-mouse-model-by-regulating-t-cell-polarization
#3
Chenghong Sun, Jian Yang, Mingzhi Wang, Lihong Pan, Jingchun Yao, Shenglan Wang, Na Guo, Chunyan Li, Guimin Zhang
Previous studies have shown that icaritin (ICT) has significant protective effects on immune thrombocytopenia (ITP), and the present study aimed to discuss the mechanism of this protective effect from the aspect of regulating T-cell polarization by an antibody-induced ITP mice model. Mice were given rat anti-mouse CD41 antibody (MWReg30) by intraperitoneal injection for 7 d to produce ITP model. At the same time, ICT was administrated at 10 mg/kg/d orally for 9 d. Peripheral blood platelets were counted by hematology analyzer...
September 22, 2017: Planta Medica
https://www.readbyqxmd.com/read/28938452/dose-specific-effects-of-di-isononyl-phthalate-on-the-endocannabinoid-system-and-on-liver-of-female-zebrafish
#4
Isabel Forner-Piquer, Francesca Maradonna, Giorgia Gioacchini, Stefania Santangeli, Marco Allarà, Fabiana Piscitelli, Hamid R Habibi, Vincenzo Di Marzo, Oliana Carnevali
Phthalates, used as plasticizers, have become a ubiquitous contaminant and have been reported for their potential to induce toxicity in living organisms. Among them, the Di-isononyl phthalate (DiNP) has been recently used to replace the bis(2-ethylhexyl) phthalate (DEHP). Nowadays, there is evidence that DiNP is an Endocrine Disrupting Chemical (EDC), however little is known about its effects on the Endocannabinoid System (ECS) and lipid metabolism. Hence, the aim of our study was to investigate the effects of DiNP on the ECS in zebrafish liver and brain and on hepatic lipid storage...
August 14, 2017: Endocrinology
https://www.readbyqxmd.com/read/28938337/pulse-pressure-is-associated-with-plasma-amyloid-%C3%AE-transport-dysfunction
#5
Yu Jiang, Suhang Shang, Pei Li, Chen Chen, Liangjun Dang, Jin Wang, Kang Huo, Meiying Deng, Jingyi Wang, Qiumin Qu
OBJECTIVE: Increased pulse pressure (PP) has been implicated in the development and progression of Alzheimer's disease in middle-aged and elderly adults. Considering the close relationship between peripheral amyloid-β clearance and brain amyloid-β deposition, we investigated the potential association between PP and plasma amyloid-β transport function. METHODS: In this cross-sectional study, a total of 1118 participants underwent a health assessment and quantification of plasma amyloid-β and amyloid-β transporter expression...
September 21, 2017: Journal of Hypertension
https://www.readbyqxmd.com/read/28938216/efficient-removal-of-priority-hazardous-priority-and-emerging-pollutants-with-prunus-armeniaca-functionalized-biochar-from-aqueous-wastes-experimental-optimization-and-modeling
#6
Maja Turk Sekulić, Sabolč Pap, Zoran Stojanović, Nikola Bošković, Jelena Radonić, Tatjana Šolević Knudsen
This paper investigates the ability of the phosphoric acid functionalized Prunus armeniaca stones biochar (AsPhA) prepared by thermochemical activation to remove lead (Pb(2+)), cadmium (Cd(2+)), nickel (Ni(2+)), naproxen and chlorophenols from aqueous wastes. The engineered biochar was characterized using the Scanning Electron Microscopy, Energy-dispersive X-ray Spectroscopy, Fourier Transform Infrared Spectroscopy and Brunauer, Emmett and Teller technique. The batch studies were performed by varying the initial pH of the solution (2-9), adsorbent dosage (0...
September 19, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28938212/the-problem-of-identifying-arsenic-anomalies-in-the-basin-of-sahand-dam-through-risk-based-soft-modelling
#7
Ata Allah Nadiri, Fariba Sadeghi Aghdam, Rahman Khatibi, Asghar Asghari Moghaddam
An investigation is undertaken to identify arsenic anomalies at the complex of Sahand dam, East Azerbaijan, northwest Iran. The complex acts as a system, in which the impounding reservoir catalyses system components related to Origin-Source-Pathways-Receptor-Consequence (OSPRC) viewed as a risk system. This 'conceptual framework' overlays a 'perceptual model' of the physical system, in which arsenic with geogenic origins diffused into the formations through extensive fractures swept through the region during the Miocene era...
September 19, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28938196/sorption-of-uranium-vi-onto-hydrous-ferric-oxide-modified-zeolite-assessment-of-the-effect-of-ph-contact-time-temperature-selected-cations-and-anions-on-sorbent-interactions
#8
Pfano Mathews Nekhunguni, Nikita Tawanda Tavengwa, Hlanganani Tutu
Zeolites are commonly used as adsorbents for metal removal in most applications e.g. in wastewater. However, the ubiquity of iron in such systems may, in the long-term, distort the true interactions and mechanisms of contaminant removal as a result of modification of the zeolite surface. In this study, this potential phenomenon was assessed for the removal of uranium(VI) from aqueous solution by hydrous ferric oxide-modified zeolite (HFOMZ). This was prepared by precipitating iron hydroxide (the common precipitate of iron in aqueous systems) onto zeolite...
September 19, 2017: Journal of Environmental Management
https://www.readbyqxmd.com/read/28938017/a-highly-conserved-amino-acid-in-vp1-regulates-maturation-of-enterovirus-71
#9
Yong-Xin Zhang, Yu-Ming Huang, Quan-Jie Li, Xiao-Yu Li, Yong-Dong Zhou, Fei Guo, Jin-Ming Zhou, Shan Cen
Enterovirus 71 (EV71) is the major causative agent of hand, foot and mouth disease (HFMD) in children, causing severe clinical outcomes and even death. Here, we report an important role of the highly conserved alanine residue at position 107 in the capsid protein VP1 (VP1A107) in the efficient replication of EV71. Substitutional mutations of VP1A107 significantly diminish viral growth kinetics without significant effect on viral entry, expression of viral genes and viral production. The results of mechanistic studies reveal that VP1A107 regulates the efficient cleavage of the VP0 precursor during EV71 assembly, which is required, in the next round of infection, for the transformation of the mature virion (160S) into an intermediate or A-particle (135S), a key step of virus uncoating...
September 22, 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/28938007/neuropilin-1-modulates-tgf%C3%AE-signaling-to-drive-glioblastoma-growth-and-recurrence-after-anti-angiogenic-therapy
#10
Sam C Kwiatkowski, Paola A Guerrero, Shinya Hirota, Zhihua Chen, John E Morales, Manish Aghi, Joseph H McCarty
Glioblastoma (GBM) is a rapidly progressive brain cancer that exploits the neural microenvironment, and particularly blood vessels, for selective growth and survival. Anti-angiogenic agents such as the vascular endothelial growth factor-A (VEGF-A) blocking antibody bevacizumab yield short-term benefits to patients due to blood vessel regression and stabilization of vascular permeability. However, tumor recurrence is common, and this is associated with acquired resistance to bevacizumab. The mechanisms that drive acquired resistance and tumor recurrence in response to anti-angiogenic therapy remain largely unknown...
2017: PloS One
https://www.readbyqxmd.com/read/28937992/efficient-generation-of-mutations-mediated-by-crispr-cas9-in-the-hairy-root-transformation-system-of-brassica-carinata
#11
Thomas W Kirchner, Markus Niehaus, Thomas Debener, Manfred K Schenk, Marco Herde
A protocol for the induction of site-directed deletions and insertions in the genome of Brassica carinata with CRISPR is described. The construct containing the Cas9 nuclease and the guide RNA (gRNA) was delivered by the hairy root transformation technique, and a successful transformation was monitored by GFP fluorescence. PAGE analysis of an amplified region, presumably containing the deletions and insertions, demonstrated up to seven different indels in one transgenic root and in all analyzed roots a wildtype allele of the modified gene was not detectable...
2017: PloS One
https://www.readbyqxmd.com/read/28937982/protein-complex-prediction-via-dense-subgraphs-and-false-positive-analysis
#12
Cecilia Hernandez, Carlos Mella, Gonzalo Navarro, Alvaro Olivera-Nappa, Jaime Araya
Many proteins work together with others in groups called complexes in order to achieve a specific function. Discovering protein complexes is important for understanding biological processes and predict protein functions in living organisms. Large-scale and throughput techniques have made possible to compile protein-protein interaction networks (PPI networks), which have been used in several computational approaches for detecting protein complexes. Those predictions might guide future biologic experimental research...
2017: PloS One
https://www.readbyqxmd.com/read/28937961/regulation-of-chromatin-assembly-and-cell-transformation-by-formaldehyde-exposure-in-human-cells
#13
Danqi Chen, Lei Fang, Shenglin Mei, Hongjie Li, Xia Xu, Thomas L Des Marais, Kun Lu, X Shirley Liu, Chunyuan Jin
BACKGROUND: Formaldehyde (FA) is an environmental and occupational chemical carcinogen. Recent studies have shown that exogenous FA causes only a modest increase in DNA adduct formation compared with the amount of adducts formed by endogenous FA, raising the possibility that epigenetic mechanisms may contribute to FA-mediated carcinogenicity. OBJECTIVES: We investigated the effects of FA exposure on histone modifications and chromatin assembly. We also examined the role of defective chromatin assembly in FA-mediated transcription and cell transformation...
September 21, 2017: Environmental Health Perspectives
https://www.readbyqxmd.com/read/28937948/textures-as-probes-of-visual-processing
#14
Jonathan D Victor, Mary M Conte, Charles F Chubb
Visual textures are a class of stimuli with properties that make them well suited for addressing general questions about visual function at the levels of behavior and neural mechanism. They have structure across multiple spatial scales, they put the focus on the inferential nature of visual processing, and they help bridge the gap between stimuli that are analytically convenient and the complex, naturalistic stimuli that have the greatest biological relevance. Key questions that are well suited for analysis via visual textures include the nature and structure of perceptual spaces, modulation of early visual processing by task, and the transformation of sensory stimuli into patterns of population activity that are relevant to perception...
September 15, 2017: Annual Review of Vision Science
https://www.readbyqxmd.com/read/28937917/head-impact-exposure-measured-in-a-single-youth-football-team-during-practice-drills
#15
Mireille E Kelley, Joeline M Kane, Mark A Espeland, Logan E Miller, Alexander K Powers, Joel D Stitzel, Jillian E Urban
OBJECTIVE This study evaluated the frequency, magnitude, and location of head impacts in practice drills within a youth football team to determine how head impact exposure varies among different types of drills. METHODS On-field head impact data were collected from athletes participating in a youth football team for a single season. Each athlete wore a helmet instrumented with a Head Impact Telemetry (HIT) System head acceleration measurement device during all preseason, regular season, and playoff practices...
September 12, 2017: Journal of Neurosurgery. Pediatrics
https://www.readbyqxmd.com/read/28937861/increased-suicides-during-new-year-but-not-during-christmas-in-sweden-analysis-of-cause-of-death-data-2006-2015
#16
Gergö Hadlaczky, Sebastian Hökby
BACKGROUND: Previous studies have investigated the impact of the Christmas and New Year holiday on suicide rates. However, no such data has yet been published on Swedish suicides. AIMS: To examine the occurrence of suicides on these dates in Sweden between 2006 and 2015. METHODS: The suicide count for each date between December 15th and January 15th was obtained from the Swedish cause of death registry. The observations were transformed to Z-scores to enable calculation of p-values...
September 22, 2017: Nordic Journal of Psychiatry
https://www.readbyqxmd.com/read/28937850/environmental-transport-of-endogenous-dairy-manure-estrogens
#17
Inna E Popova, Matthew J Morra
Although estrogens originating from dairy manure applied to agricultural soils as a fertilizer can potentially contaminate surface water and groundwater, the variables that control transport are poorly understood. Our objective was to assess the potential for off-site movement of endogenous dairy cattle estrogens when manure is applied on fields at agronomically relevant fertilization rates. Estrone (E1), 17α-estradiol (α-E2), and 17β-estradiol (β-E2) were used in laboratory sorption, desorption, and transformation incubations with both manure and an agriculturally relevant soil...
September 22, 2017: Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes
https://www.readbyqxmd.com/read/28937766/approach-toward-the-understanding-of-coupling-mechanism-for-edc-reagent-in-solvent-free-mechanosynthesis
#18
Aneta Wróblewska, Piotr Paluch, Ewelina Wielgus, Grzegorz Bujacz, Marta K Dudek, Marek J Potrzebowski
A unique approach in mechanosynthesis, joining solid-state NMR spectroscopy, X-ray crystallography, and theoretical calculations, is employed for the first time to study the mechanism of the formation of the C-N amide bond using EDC·HCl as a coupling reagent. It has been proved that EDC·HCl, which in the crystal lattice exists exclusively in the cyclic form (X-ray data), easily undergoes transformation to a pseudocyclic stable intermediate in reaction with carboxylic acid forming a low-melt phase (differential scanning calorimetry, solid-state NMR)...
September 22, 2017: Organic Letters
https://www.readbyqxmd.com/read/28937762/projected-commutator-diis-method-for-accelerating-hybrid-functional-electronic-structure-calculations
#19
Wei Hu, Lin Lin, Chao Yang
The commutator direct inversion of the iterative subspace (commutator DIIS or C-DIIS) method developed by Pulay is an efficient and the most widely used scheme in quantum chemistry to accelerate the convergence of self consistent field (SCF) iterations in Hartree-Fock theory and Kohn-Sham density functional theory. The C-DIIS method requires the explicit storage of the density matrix, the Fock matrix and the commutator matrix. Hence the method can only be used for systems with a relatively small basis set, such as the Gaussian basis set...
September 22, 2017: Journal of Chemical Theory and Computation
https://www.readbyqxmd.com/read/28937739/applications-of-iridium-catalyzed-asymmetric-allylic-substitution-reactions-in-target-oriented-synthesis
#20
Jianping Qu, Günter Helmchen
Metal catalyzed allylic substitution is a cornerstone of organometallic and synthetic chemistry. Enantioselective versions have been developed with catalysts derived from transition metals, most notably molybdenum, nickel, ruthenium, rhodium, iridium, palladium, and copper. The palladium- and the iridium-catalyzed versions have turned out to be particularly versatile in organic synthesis because of the very broad scope of the nucleophile and great functional group compatibility. Assets of the iridium-catalyzed reaction are the formation of branched, chiral products from simple monosubstituted allylic substrates, high degrees of regio- and enantioselectivity, and use of modular, readily available chiral ligands...
September 22, 2017: Accounts of Chemical Research
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