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(ACL) AND (Biomarkers)

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https://www.readbyqxmd.com/read/29136827/biobarcode-assay-for-the-oral-anticoagulant-acenocoumarol
#1
Marta Broto, J Pablo Salvador, Roger Galve, M Pilar Marco
A novel approach for therapeutic drug monitoring of oral anticoagulants (OA) in clinical samples is reported, based on a NP-based biobarcode assay. The proposed strategy uses specific antibodies for acenocumarol (ACL) covalently bound to magnetic particles (pAb236-MP) and a bioconjugate competitor (hACL-BSA) linked to encoded polystyrene probes (hACL-BSA-ePSP) on a classical competitive immunochemical format. By using this scheme ACL can be detected in low nM range (LOD, 0.96 ± 0.26, N = 3, in buffer) even in complex samples such as serum or plasma (LOD 4 ± 1)...
February 1, 2018: Talanta
https://www.readbyqxmd.com/read/29106758/transcriptomic-analysis-of-synovial-extracellular-rna-following-knee-trauma-a-pilot-study
#2
Anthony J Griswold, Jose Perez, Karen Nuytemans, Thomas Strong, Liyong Wang, Danica D Vance, Hayley Ennis, Marvin K Smith, Thomas M Best, Jeffery M Vance, Margaret A Pericak-Vance, Lee D Kaplan
Traumatic knee injuries often result in damage to articular cartilage and other joint structures. Such trauma is a strong risk factor for the future development and progression of osteoarthritis (OA). The molecular mechanisms and signaling pathways modulating response to knee joint trauma remain unclear. Moreover, investigations of biomarkers influencing responses have been targeted rather than broad, unbiased discovery studies. Herein we characterize the complete complement of extracellular RNA (exRNA) in the synovial fluid of 14 subjects following knee injury...
November 6, 2017: Journal of Orthopaedic Research: Official Publication of the Orthopaedic Research Society
https://www.readbyqxmd.com/read/28921609/biomarkers-for-equine-joint-injury-and-osteoarthritis
#3
REVIEW
C Wayne McIlwraith, Christopher E Kawcak, David D Frisbie, Christopher B Little, Peter D Clegg, Mandy J Peffers, Morten A Karsdal, Stina Ekman, Sheila Laverty, Richard A Slayden, Linda J Sandell, L Stefan Lohmander, Virginia B Kraus
We report the results of a symposium aimed at identifying validated biomarkers that can be used to complement clinical observations for diagnosis and prognosis of joint injury leading to equine osteoarthritis (OA). Biomarkers might also predict pre-fracture change that could lead to catastrophic bone failure in equine athletes. The workshop was attended by leading scientists in the fields of equine and human musculoskeletal biomarkers to enable cross-disciplinary exchange and improve knowledge in both. Detailed proceedings with strategic planning was written, added to, edited and referenced to develop this manuscript...
September 16, 2017: Journal of Orthopaedic Research: Official Publication of the Orthopaedic Research Society
https://www.readbyqxmd.com/read/28903010/effect-of-loading-on-in-vivo-tibiofemoral-and-patellofemoral-kinematics-of-healthy-and-acl-reconstructed-knees
#4
Jarred M Kaiser, Michael F Vignos, Richard Kijowski, Geoffrey Baer, Darryl G Thelen
BACKGROUND: Although knees that have undergone anterior cruciate ligament reconstruction (ACLR) often exhibit normal laxity on clinical examination, abnormal kinematic patterns have been observed when the joint is dynamically loaded during whole body activity. This study investigated whether abnormal knee kinematics arise with loading under isolated dynamic movements. HYPOTHESIS: Tibiofemoral and patellofemoral kinematics of ACLR knees will be similar to those of the contralateral uninjured control knee during passive flexion-extension, with bilateral differences emerging when an inertial load is applied...
September 1, 2017: American Journal of Sports Medicine
https://www.readbyqxmd.com/read/28629188/serum-biomarkers-for-discrimination-between-hepatitis-c-related-arthropathy-and-early-rheumatoid-arthritis
#5
Isabela Siloşi, Lidia Boldeanu, Viorel Biciuşcă, Maria Bogdan, Carmen Avramescu, Citto Taisescu, Vlad Padureanu, Mihail Virgil Boldeanu, Anica Dricu, Cristian Adrian Siloşi
In the present study, we aimed to estimate the concentrations of cytokines (interleukin 6, IL-6, tumor necrosis factor-α, TNF-α) and auto-antibodies (rheumatoid factor IgM isotype, IgM-RF, antinuclear auto-antibodies, ANA, anti-cyclic citrullinated peptide antibodies IgG isotype, IgG anti-CCP3.1, anti-cardiolipin IgG isotype, IgG anti-aCL) in serum of patients with eRA (early rheumatoid arthritis) and HCVrA (hepatitis C virus-related arthropathy) and to assess the utility of IL-6, TNF-α together with IgG anti-CCP and IgM-RF in distinguishing between patients with true eRA and HCVrA, in the idea of using them as differential immunomarkers...
June 19, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28522220/surgical-reconstruction-of-ruptured-anterior-cruciate-ligament-prolongs-trauma-induced-increase-of-inflammatory-cytokines-in-synovial-fluid-an-exploratory-analysis-in-the-kanon-trial
#6
S Larsson, A Struglics, L S Lohmander, R Frobell
OBJECTIVE: Prospectively monitor how treatment of acutely ruptured anterior cruciate ligament (ACL) affects biomarkers of inflammation and proteolytic degradation over 5 years. DESIGN: We studied 119 subjects with acute ACL injury from the randomized controlled knee anterior cruciate ligament, non-surgical versus surgical treatment (KANON)-trial (Clinical trial ISRCTN 84752559) who had synovial fluid, serum and urine samples available from at least two out of six visits over 5 years after acute ACL rupture...
September 2017: Osteoarthritis and Cartilage
https://www.readbyqxmd.com/read/28341873/exploring-the-plasmatic-platelet-activating-factor-acetylhydrolase-activity-in-patients-with-anti-phospholipid-antibodies
#7
Martina Fabris, Adriana Cifù, Cinzia Pistis, Massimo Siega-Ducaton, Desrè Ethel Fontana, Roberta Giacomello, Elio Tonutti, Francesco Curcio
PURPOSE: To explore the role of plasmatic platelet-activating factor acetylhydrolase (PAF-AH), a marker of cardiovascular risk, in patients with anti-phospholipid antibodies (aPL). METHODS: PAF-AH activity was assessed in a series of 167 unselected patients screened for aPL in a context of thrombotic events, risk of thrombosis or obstetric complications and in 77 blood donors. RESULTS: 116/167 patients showed positive results for at least one aPL among IgG/IgM anti-prothrombin/phosphatidylserine (aPS/PT), anti-cardiolipin (aCL), anti-beta2-glycoprotein I (aβ2GPI) or lupus anticoagulant (LAC), while 51/167 patients resulted aPL-negative...
December 2017: Auto- Immunity Highlights
https://www.readbyqxmd.com/read/28284998/acute-mobilization-and-migration-of-bone-marrow-derived-stem-cells-following-anterior-cruciate-ligament-rupture
#8
T Maerz, M Fleischer, M D Newton, A Davidson, M Salisbury, P Altman, M D Kurdziel, K Anderson, A Bedi, K C Baker
OBJECTIVE: Little is known regarding acute local and systemic processes following anterior cruciate ligament (ACL) rupture. No study has elucidated whether bone marrow-derived mesenchymal stem cells (MSCs) are mobilized into circulation and recruited to the injured joint. METHODS: In Part 1, Lewis rats were randomized to noninvasive ACL rupture (Rupture) or non-injured (Control) (n = 6/group). After 72 h, whole blood MSC concentration was assessed using flow cytometry...
August 2017: Osteoarthritis and Cartilage
https://www.readbyqxmd.com/read/28194829/diffusion-tensor-imaging-of-the-anterior-cruciate-ligament-graft
#9
Pieter Van Dyck, Martijn Froeling, Eline De Smet, Pim Pullens, Michaël Torfs, Peter Verdonk, Jan Sijbers, Paul M Parizel, Ben Jeurissen
PURPOSE: A great need exists for objective biomarkers to assess graft healing following ACL reconstruction to guide the time of return to sports. The purpose of this study was to evaluate the feasibility and reliability of diffusion tensor imaging (DTI) to delineate the anterior cruciate ligament (ACL) graft and to investigate its diffusion properties using a clinical 3T scanner. MATERIALS AND METHODS: DTI of the knee (b = 0, 400, and 800 s/mm(2) , 10 diffusion directions, repeated 16 times for a total of 336 diffusion-weighted volumes) was performed at 3T in 17 patients between 3 and 7 months (mean, 4 months) following ACL reconstruction...
November 2017: Journal of Magnetic Resonance Imaging: JMRI
https://www.readbyqxmd.com/read/28176368/pharmacological-inhibition-of-myostatin-protects-against-skeletal-muscle-atrophy-and-weakness-after-anterior-cruciate-ligament-tear
#10
Caroline Nw Wurtzel, Jonathan P Gumucio, Jeremy A Grekin, Roger K Khouri, Alan J Russell, Asheesh Bedi, Christopher L Mendias
Anterior cruciate ligament (ACL) tears are among the most frequent knee injuries in sports medicine, with tear rates in the US up to 250,000 per year. Many patients who suffer from ACL tears have persistent atrophy and weakness even after considerable rehabilitation. Myostatin is a cytokine that directly induces muscle atrophy, and previous studies rodent models and patients have demonstrated an upregulation of myostatin after ACL tear. Using a preclinical rat model, our objective was to determine if the use of a bioneutralizing antibody against myostatin could prevent muscle atrophy and weakness after ACL tear...
November 2017: Journal of Orthopaedic Research: Official Publication of the Orthopaedic Research Society
https://www.readbyqxmd.com/read/28147215/attenuated-synovial-fluid-ghrelin-levels-are-linked-with-cartilage-damage-meniscus-injury-and-clinical-symptoms-in-patients-with-knee-anterior-cruciate-ligament-deficiency
#11
Yu-Cong Zou, Liang-Hua Chen, Yong-Liang Ye, Guang-Gang Yang, Zheng Mao, Dan-Dan Liu, Jun-Qi Chen, Jing-Jie Chen, Gang Liu
BACKGROUND: The meniscus injury and post-traumatic knee osteoarthritis (PTOA) following anterior cruciate ligament (ACL) lesions often cause great burdens to patients. Ghrelin, a recently identified 28-amino-acid peptide, has been shown to inhibit inflammation and perform as a growth factor for chondrocyte. OBJECTIVE: This study was aimed at investigating ghrelin concentration in synovial fluid and its association with the degree of meniscus injury, articular degeneration, and clinical severity in patients suffering from anterior cruciate ligament (ACL) deficiency...
December 2016: Discovery Medicine
https://www.readbyqxmd.com/read/28146402/a-multicenter-study-of-early-anti-inflammatory-treatment-in-patients-with-acute-anterior-cruciate-ligament-tear
#12
RANDOMIZED CONTROLLED TRIAL
Christian Lattermann, Cale A Jacobs, Mary Proffitt Bunnell, Laura J Huston, Lee G Gammon, Darren L Johnson, Emily K Reinke, Janet L Huebner, Virginia B Kraus, Kurt P Spindler
BACKGROUND: It is increasingly recognized that biochemical abnormalities of the joint precede radiographic abnormalities of posttraumatic osteoarthritis (PTOA) by as much as decades. A growing body of evidence strongly suggests that the progression from anterior cruciate ligament (ACL) injury to PTOA is multifactorial, involving the interplay between biomechanical disturbances and biochemical homeostasis of articular cartilage. PURPOSE: The purposes of this randomized study using an acute ACL injury model were to (1) evaluate the natural progression of inflammatory and chondrodegenerative biomarkers, (2) evaluate the relationship between subjective reports of pain and inflammatory and chondrodegenerative biomarkers, and (3) determine if postinjury arthrocentesis and corticosteroid injection offer the ability to alter this biochemical cascade...
February 2017: American Journal of Sports Medicine
https://www.readbyqxmd.com/read/28130030/biomarker-changes-in-anterior-cruciate-ligament-deficient-knees-compared-with-healthy-controls
#13
Daniel J Kaplan, Vanessa G Cuellar, Laith M Jazrawi, Eric J Strauss
PURPOSE: To establish how synovial fluid biomarker concentrations change in patients after anterior cruciate ligament (ACL) tears, with and without associated cartilage injury, with comparisons made to healthy controls. METHODS: Patients were prospectively enrolled between January 2013 and December 2014. Inclusion criteria included any patient undergoing knee arthroscopy. Patients with a confirmed ACL tear were allocated to either the ACL tear with cartilage injury group or the ACL tear without cartilage injury group based on intraoperative assessment...
January 24, 2017: Arthroscopy: the Journal of Arthroscopic & related Surgery
https://www.readbyqxmd.com/read/28090441/principal-component-analysis-t1%C3%AF-voxel-based-relaxometry-of-the-articular-cartilage-a-comparison-of-biochemical-patterns-in-osteoarthritis-and-anterior-cruciate-ligament-subjects
#14
Valentina Pedoia, Colin Russell, Allison Randolph, Xiaojuan Li, Sharmila Majumdar
BACKGROUND: Quantitative MR, including T1ρ mapping, has been extensively used to probe early biochemical changes in knee articular cartilage of subjects with osteoarthritis (OA) and others at risk for cartilage degeneration, such as those with anterior cruciate ligament (ACL) injury and reconstruction. However, limited studies have been performed aimed to assess the spatial location and patterns of T1ρ. In this study we used a novel voxel-based relaxometry (VBR) technique coupled with principal component analysis (PCA) to extract relevant features so as to describe regional patterns and to investigate their similarities and differences in T1ρ maps in subjects with OA and subjects six months after ACL reconstruction (ACLR)...
December 2016: Quantitative Imaging in Medicine and Surgery
https://www.readbyqxmd.com/read/27753626/a-unique-antiphospholipid-assay-recognizing-phospholipid-mixture-compared-with-criteria-antiphospholipid-immunoassays-in-lupus-patients
#15
COMPARATIVE STUDY
Y Zuo, R Willis, E Papalardo, M Petri, E N Harris, A Schleh, K DeCeulaer, M Smikle, L M Vilá, J D Reveille, G S Alarcón, E B Gonzalez
Background While essential for the classification of antiphospholipid syndrome (APS), anticardiolipin (aCL) assays lack specificity and anti-β2glycoproteinI (anti-β2GPI) assays lack sensitivity in this regard. Our aim was to perform a comparative analysis of the APhL ELISA assay (IgG/IgM) and criteria antiphospholipid (aPL) immunoassays in identifying APS-related clinical manifestations in a large group of patients with systemic lupus erythematosus (SLE). Methods Serum samples from 1178 patients from the Hopkins ( n = 543), LUMINA ( n = 588) and Jamaican SLE cohorts ( n = 47) were examined for IgG/IgM positivity in aCL (in-house), anti-β2GPI (two commercial kits) and APhL (Louisville APL) ELISA assays...
May 2017: Lupus
https://www.readbyqxmd.com/read/27563836/composite-metric-r2-%C3%A2-%C3%A2-r1%C3%AF-1-t2-%C3%A2-%C3%A2-1-t1%C3%AF-as-a-potential-mr-imaging-biomarker-associated-with-changes-in-pain-after-acl-reconstruction-a-six-month-follow-up
#16
MULTICENTER STUDY
Colin Russell, Valentina Pedoia, Sharmila Majumdar
This study looked to investigate a new quantitative metric, R2  - R1ρ (1/T2  - 1/T1ρ ), using magnetic resonance (MR) images and voxel-based relaxometry (VBR) for detecting early cartilage degeneration and explore the association with patient-reported outcomes measures (PROMs) in patients 6 months after ACL reconstruction. Sixty-four patients from three sites were bilaterally scanned on a 3T MR with a combined T1ρ /T2 protocol to calculate R1ρ (1/T1ρ ) and R2 (1/T2 ) values at baseline and 6 months after reconstructive surgery...
March 2017: Journal of Orthopaedic Research: Official Publication of the Orthopaedic Research Society
https://www.readbyqxmd.com/read/27557479/analysis-of-the-articular-cartilage-t1%C3%AF-and-t2-relaxation-times-changes-after-acl-reconstruction-in-injured-and-contralateral-knees-and-relationships-with-bone-shape
#17
Valentina Pedoia, Favian Su, Keiko Amano, Qi Li, Charles E McCulloch, Richard B Souza, Thomas M Link, Benjamin C Ma, Xiaojuan Li
The objectives of this study were twofold: (1) to evaluate the longitudinal change in cartilage T1ρ and T2 6- and 12-months after ACL reconstruction (ACLR) in both reconstructed and intact contralateral knees with the aim of validating the role of the contralateral knee as an internal control in longitudinal studies; (2) to explore relationships between bone shape at the time of injury and the progression of T1ρ and T2 over 12-months after ACLR. T1ρ and T2 cartilage relaxation times and 3D MRI-based statistical shape modeling (SSM) of tibia and femur were computed for both knees of forty ACL-injured patients and 15 healthy controls...
March 2017: Journal of Orthopaedic Research: Official Publication of the Orthopaedic Research Society
https://www.readbyqxmd.com/read/27306867/review-of-current-understanding-of-post-traumatic-osteoarthritis-resulting-from-sports-injuries
#18
REVIEW
Andrew Carbone, Scott Rodeo
Certain types of joint injuries, common in athletes, are known to have a high association with the development of osteoarthritis (OA). Post-traumatic osteoarthritis (PTOA) is especially debilitating due to its earlier onset than traditional OA, and its predisposition to affect a younger and more active population. Five common athletic injuries have been demonstrated to be risk factors for the development of OA. These include ACL rupture, meniscus tear, glenohumeral instability, patellar dislocation, and ankle instability...
March 2017: Journal of Orthopaedic Research: Official Publication of the Orthopaedic Research Society
https://www.readbyqxmd.com/read/27279368/logistical-challenges-and-design-considerations-for-studies-using-acute-anterior-cruciate-ligament-injury-as-a-potential-model-for-early-posttraumatic-osteoarthritis
#19
RANDOMIZED CONTROLLED TRIAL
Christian Lattermann, Cale A Jacobs, Mary Proffitt Bunnell, Kate N Jochimsen, John P Abt, Emily K Reinke, Lee G Gammon, Janet L Huebner, Virginia B Kraus, Kurt P Spindler
Anterior cruciate ligament (ACL) injuries are common and lead to posttraumatic osteoarthritis (PTOA) in a high percentage of patients. Research has been ineffective in identifying successful treatment options for people suffering from symptomatic PTOA resulting in a shift of focus toward the young, ACL injured patients at risk of developing PTOA. Randomized clinical trials examining the very early phase after ACL injury are ideal to study this population; however, these trials face significant challenges regarding recruitment as well as reproducibility of patient-reported outcomes (PROs) and inflammatory and/or chondrodegenerative biomarkers associated with early PTOA...
March 2017: Journal of Orthopaedic Research: Official Publication of the Orthopaedic Research Society
https://www.readbyqxmd.com/read/27159304/the-association-between-serum-biomarkers-of-collagen-turnover-and-subsequent-anterior-cruciate-ligament-rupture
#20
Steven J Svoboda, Brett D Owens, Travis M Harvey, Patrick M Tarwater, William F Brechue, Kenneth L Cameron
BACKGROUND: No study has attempted to associate the levels of preinjury serum biomarkers of collagen turnover with the subsequent risk of anterior cruciate ligament (ACL) injury. HYPOTHESIS: Preinjury serum biomarkers of collagen turnover would be associated with the subsequent risk of ACL injury. STUDY DESIGN: Case-control study; Level of evidence, 3. METHODS: We conducted a case-control study with 45 ACL-injured cases and 45 controls matched for sex, age, height, and weight...
July 2016: American Journal of Sports Medicine
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