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https://www.readbyqxmd.com/read/29145127/effect-of-lipid-free-microalgal-biomass-and-waste-glycerol-on-growth-and-lipid-production-of-scenedesmus-obliquus-innovative-waste-recycling-for-extraordinary-lipid-production
#1
Abd El-Fatah Abomohra, Hamed Eladel, Mohamed El-Esawi, Shuang Wang, Qian Wang, Zhixia He, Yongqiang Feng, Hao Shang, Dieter Hanelt
In the present work, a novel approach of using growth medium with different substitutions of lipid-free algal hydrolysate (LFAH, 0, 5, 10 and 15%) and/or waste glycerol (WG, 0, 5, 10 and 20 g L(-1)) for enhanced biodiesel production from Scenedesmus obliquus was studied. Combination of different concentrations of WG with 15% LFAH showed the maximum significant biomass productivity, which represented 27.4, 30.5 and 28.9% over the control at combined 5, 10 and 20 g L(-1) WG, respectively. The combinations of different LFAH with 20 g L(-1) WG showed the maximum significant lipid accumulation, where lipid productivity showed its maximum significant value of 59...
November 4, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/29135651/comparison-of-the-pharmacokinetics-and-pharmacodynamics-of-single-dose-tenofovir-vaginal-film-and-gel-formulation-fame-05
#2
Jennifer A Robinson, Mark A Marzinke, Edward J Fuchs, Rahul P Bakshi, Hans M L Spiegel, Jenell S Coleman, Lisa C Rohan, Craig W Hendrix
OBJECTIVE: While pre-exposure prophylaxis with oral tenofovir (TFV) disoproxil fumarate/emtricitabine reduces HIV acquisition rates, poor adherence to and acceptability of daily vaginal gels has led to development of vaginal film formulations to improve adherence and, potentially, enable episodic use. STUDY DESIGN: In this two-arm, cross-over study of a fast-dissolving tenofovir film (40 mg) compared to a previously studied semisolid tenofovir 1% gel (40 mg), 10 healthy women received a single vaginal dose of each study product...
November 7, 2017: Journal of Acquired Immune Deficiency Syndromes: JAIDS
https://www.readbyqxmd.com/read/29114241/a-nanoscale-study-of-carbon-and-nitrogen-fluxes-in-mats-of-purple-sulfur-bacteria-implications-for-carbon-cycling-at-the-surface-of-coastal-sediments
#3
Cédric Hubas, Dominique Boeuf, Bruno Jesus, Najet Thiney, Yann Bozec, Christian Jeanthon
Mass blooms of purple sulfur bacteria growing seasonally on green stranded macroalgae have a major impact on the microbial composition and functionality of intertidal mats. To explore the active anoxygenic phototrophic community in purple bacterial mats from the Roscoff Aber Bay (Brittany, France), we conducted a combined approach including molecular and high-resolution secondary ion mass spectrometry (NanoSIMS) analyses. To investigate the dynamics of carbon and nitrogen assimilation activities, NanoSIMS was coupled with a stable isotope probing (SIP) experiment and a compound specific isotope analysis (CSIA) of fatty acid methyl ester (FAME)...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29110110/changes-and-clinical-significance-of-cd8-cd122-t-cells-in-the-peripheral-blood-of-patients-with-ankylosing-spondylitis
#4
Renfang Han, Xiao Yang, Mengya Chen, Xu Zhang, Yaping Yuan, Xingxing Hu, Mengmeng Wang, Rui Liu, Yubo Ma, Jiajia Yang, Shengqian Xu, Zongwen Shuai, Shanqun Jiang, Faming Pan
The aim of our study was to explore the relationship between circulating T cells and ankylosing spondylitis (AS) and to find the role of the CD8+CD122+ T cells in the pathogenesis and progression of AS. With the method of case-control design, flow cytometry was performed to quantitatively determine the percentage of circulating CD8(+)CD122(+) T cells in peripheral blood mononuclear cells from established AS patients and age- and gender-matched healthy controls. Serum levels of inflammatory cytokines like interleukin-2, interleukin-10, interleukin-15, TGF-β1, and TNF-ɑ were detected by enzyme-linked immunosorbent assay...
November 6, 2017: Clinical Rheumatology
https://www.readbyqxmd.com/read/29097450/clinical-outcomes-and-cost-effectiveness-of-fractional-flow-reserve-guided-percutaneous-coronary-intervention-in-patients-with-stable-coronary-artery-disease-three-year-follow-up-of-the-fame-2-trial-fractional-flow-reserve-versus-angiography-for-multivessel
#5
William F Fearon, Takeshi Nishi, Bernard De Bruyne, Derek B Boothroyd, Emanuele Barbato, Pim Tonino, Peter Jüni, Nico H J Pijls, Mark A Hlatky
BACKGROUND : Previous studies found that percutaneous coronary intervention (PCI) does not improve outcome compared with medical therapy (MT) in patients with stable coronary artery disease, but PCI was guided by angiography alone. FAME 2 trial (Fractional Flow Reserve Versus Angiography for Multivessel Evaluation) compared PCI guided by fractional flow reserve with best MT in patients with stable coronary artery disease to assess clinical outcomes and cost-effectiveness. METHODS : A total of 888 patients with stable single-vessel or multivessel coronary artery disease with reduced fractional flow reserve were randomly assigned to PCI plus MT (n=447) or MT alone (n=441)...
November 2, 2017: Circulation
https://www.readbyqxmd.com/read/29074028/effects-of-fabric-thickness-and-material-on-apparent-wet-conductive-thermal-resistance-of-knitted-fabric-skin-on-sweating-manikins
#6
Faming Wang, Dandan Lai, Wen Shi, Ming Fu
Currently, no published standard and research work have addressed the basic requirements on knitted fabric 'skin' on sweating manikins. In this study, we performed 252 experiments to investigate the influence of fabric thickness and material on the apparent 'wet' conductive (or effective) thermal resistance of the fabric 'skin' using a 'Newton' manikin. Four types of cotton fabric 'skin' (fabric thickness: 0.38, 0.54, 0.92 and 1.43mm) and three types of polyester fabric 'skin' (fabric thickness: 0.41, 0.54 and 1...
December 2017: Journal of Thermal Biology
https://www.readbyqxmd.com/read/29074024/an-investigation-of-thermophysiological-responses-of-human-while-using-four-personal-cooling-strategies-during-heatwaves
#7
Faming Wang, Wenfang Song
This study aimed to examine how human body thermophysiologically responds to four commonly used cooling strategies (i.e., electric fan cooling [AirFC], evaporative cooling clothing [EvapCC], hybrid cooling, i.e., evaporative cooling clothing combined with electric fan cooling [EvapCC&AirFC] and liquid cooling clothing [LiqCC]) during heatwaves. An adaptive 'Newton' manikin was used to predict human physiological responses while using these four cooling systems. Four heatwave conditions were selected, i.e., 36°C, 33% RH (hot-dry, denoted as HD1), 36°C, 67% RH (hot-humid, denoted as HH1), 40°C, 27% RH (extremely hot-dry, denoted as HD2) and 40°C, 54% RH (extremely hot-humid, denoted as HH2)...
December 2017: Journal of Thermal Biology
https://www.readbyqxmd.com/read/29074022/a-field-study-of-the-effectiveness-and-practicality-of-a-novel-hybrid-personal-cooling-vest-worn-during-rest-in-hong-kong-construction-industry
#8
Albert P C Chan, Ying Zhang, Faming Wang, Francis F K Wong, Daniel W M Chan
A novel hybrid cooling vest (HCV) incorporated with phase change materials (PCMs) and ventilation fans has been developed for construction workers in Hong Kong to attenuate heat stress and prevent heat-related illnesses, and its effectiveness and practicality have been validated in this study. A total of 140 wear trials involving of 140 workers were conducted in Hong Kong construction sites during the summer time. Each wear trial involves a two-day wear test, of which one day workers wore the HCV (denoted as VEST) during resting, and another day they wore traditional workwear (denoted as CON)...
December 2017: Journal of Thermal Biology
https://www.readbyqxmd.com/read/29072117/investigation-of-phyco-remediation-of-road-salt-run-off-with-marine-microalgae-nannochloropsis-gaditana
#9
Roopa Devasya, Amarjeet Bassi
Phyco-remediation is an environmental friendly method, which involves the application of beneficial microalgae to treat wastewater-containing pollutants for a diverse range of conditions. Several industrial processes generate hyper saline wastewater, which is a significant challenge for conventional wastewater treatment, and the disposal of saline waters also has a significant negative impact on the environment. Road salt run-off is one such saline wastewater stream not currently treated and one that contributes significantly to negatively impacting receiving bodies of water...
October 26, 2017: Environmental Technology
https://www.readbyqxmd.com/read/29067227/pseudomonas-sp-bup6-produces-a-thermotolerant-alkaline-lipase-with-trans-esterification-efficiency-in-producing-biodiesel
#10
Prakasan Priji, Sreedharan Sajith, Panichikkal Abdul Faisal, Sailas Benjamin
The present study describes the characteristics of a thermotolerant and alkaline lipase secreted by Pseudomonas sp. BUP6, a novel rumen bacterium isolated from Malabari goat, and its trans-esterification efficiency in producing biodiesel from used cooking oil (UCO). The extracellular lipase was purified to homogeneity (35.8 times purified with 14.8% yield) employing (NH4)2SO4 salt precipitation and Sephadex G-100 chromatography. The apparent molecular weight of this lipase on SDS-PAGE was 35 kDa, the identity of which was further confirmed by MALDI-TOF/MS...
December 2017: 3 Biotech
https://www.readbyqxmd.com/read/29066503/chromogranin-a-as-circulating-marker-for-diagnosis-and-management-of-neuroendocrine-neoplasms-more-flaws-than-fame
#11
Vincenzo Marotta, Maria Chiara Zatelli, Concetta Sciammarella, Maria Rosaria Ambrosio, Marta Bondanelli, Annamaria A L Colao, Antongiulio Faggiano
Owing to the heterogeneity of neuroendocrine neoplasms (NENs), the availability of reliable circulating markers is critical for improving diagnostics, prognostic stratification, follow-up, and definition of treatment strategy. This review is focused on Chromogranin A (CgA), a hydrophilic glycoprotein present in large dense core vesicles of neuroendocrine cells. Despite being long identified as the most useful NEN-related circulating marker, clinical application of CgA is controversial. CgA assays still lack of standardization, thus hampering not only clinical management but also the comparison between different analyses...
October 24, 2017: Endocrine-related Cancer
https://www.readbyqxmd.com/read/29065878/mutants-of-yarrowia-lipolytica-ncim-3589-grown-on-waste-cooking-oil-as-a-biofactory-for-biodiesel-production
#12
Gouri Katre, Namasvi Ajmera, Smita Zinjarde, Ameeta RaviKumar
BACKGROUND: Oleaginous yeasts are fast emerging as a possible feedstock for biodiesel production. Yarrowia lipolytica, a model oleaginous yeast is known to utilize a variety of hydrophobic substrates for lipid accumulation including waste cooking oil (WCO). Approaches to increase lipid content in this yeast include metabolic engineering which requires manipulation of multiple genes in the lipid biosynthesis pathway. A classical and cost-effective approach, namely, random chemical mutagenesis on the yeast can lead to increased production of biodiesel as is explored here...
October 24, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/29037768/potential-of-macroalgae-for-biodiesel-production-screening-and-evaluation-studies
#13
Abd El-Fatah Abomohra, Amal Hamed El-Naggar, Areej Ali Baeshen
Nowadays, biofuel production is a fast expanding industry and is facing a growing dilemma about a feedstock source capable of keeping up with demand. Recently, macroalgae have been attracting a wide attention as a source for biofuel. In the present study, ten macroalgae were collected and screened as biodiesel feedstocks. As a result of their high biomass production and relatively high lipid content, Ulva lactuca, Padina boryana and Ulva intestinalis showed the highest significant lipids and fatty acid methyl esters (FAMEs) areal productivities among the studied species...
October 13, 2017: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/29036516/a-two-stage-approach-of-gene-network-analysis-for-high-dimensional-heterogeneous-data
#14
Sangin Lee, Faming Liang, Ling Cai, Guanghua Xiao
Gaussian graphical models have been widely used to construct gene regulatory networks from gene expression data. Most existing methods for Gaussian graphical models are designed to model homogeneous data, assuming a single Gaussian distribution. In practice, however, data may consist of gene expression studies with unknown confounding factors, such as study cohort, microarray platforms, experimental batches, which produce heterogeneous data, and hence lead to false positive edges or low detection power in resulting network, due to those unknown factors...
July 18, 2017: Biostatistics
https://www.readbyqxmd.com/read/29031116/evaluation-of-fluoride-bioremediation-and-production-of-biomolecules-by-living-cyanobacteria-under-fluoride-stress-condition
#15
Gargi Biswas, Sohini Guha Thakurta, Jitamanyu Chakrabarty, Kalyan Adhikari, Susmita Dutta
Application of microalgae for defluoridation has gained interest in recent years. In the present study, bioremediation of fluoride using living cyanobacteria, Starria zimbabweensis, collected from wastewater of coke-oven effluent treatment plant, Durgapur, India, has been investigated. Initially, the cyanobacterial strain was grown in BG11 medium at 25°C, 45μmol/m(2)/s irradiation in 18h: 6h light:dark cycle in an algal incubator. Samples were withdrawn after 2 days interval and analyzed for its dry biomass and lipid content...
October 11, 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/29025592/significantly-mutated-genes-and-regulatory-pathways-in-sclc-a-meta-analysis
#16
Varsha Sundaresan, Victor T Lin, Faming Liang, Frederic J Kaye, Reika Kawabata-Iwakawa, Kouya Shiraishi, Takashi Kohno, Jun Yokota, Lei Zhou
Small cell lung cancer (SCLC) accounts for approximately 15% of all lung cancers and demands effective targeted therapeutic strategies. In this meta-analysis study, we aim to identify significantly mutated genes and regulatory pathways to help us better understand the progression of SCLC and to identify potential biomarkers. Besides ranking genes based on their mutation frequencies, we sought to identify statistically significant mutations in SCLC with the MutSigCV software. Our analysis identified several genes with relatively low mutation frequency, including PTEN, as highly significant (p < 0...
October 2017: Cancer Genetics
https://www.readbyqxmd.com/read/29023557/stard-2015-was-reproducible-in-a-large-set-of-studies-on-glaucoma
#17
Gianni Virgili, Manuele Michelessi, Alba Miele, Francesco Oddone, Giada Crescioli, Valeria Fameli, Ersilia Lucenteforte
AIM: To investigate the reproducibility of the updated Standards for the Reporting of Diagnostic Accuracy Studies tool (STARD 2015) in a set of 106 studies included in a Cochrane diagnostic test accuracy (DTA) systematic review of imaging tests for diagnosing manifest glaucoma. METHODS: One senior rater with DTA methodological and clinical expertise used STARD 2015 on all studies, and each of three raters with different training profiles assessed about a third of the studies...
2017: PloS One
https://www.readbyqxmd.com/read/28985620/serum-levels-of-leptin-adiponectin-and-resistin-in-patients-with-ankylosing-spondylitis-a-systematic-review-and-meta-analysis
#18
REVIEW
Jiajia Yang, Xu Zhang, Yubo Ma, Meng Wu, Xingxing Hu, Renfang Han, Yaping Yuan, Mengmeng Wang, Mengya Chen, Shanqun Jiang, Jingjing Tong, Shengqian Xu, Jianhua Xu, Zongwen Shuai, Yanfeng Zou, Faming Pan
OBJECTIVES: Various studies have researched the serum levels of leptin, adiponectin and resistin in patients with ankylosing spondylitis (AS), but the results were inconclusive. The purpose of this study was to systematically evaluate the correlations between serum levels of these adipokines and AS. METHODS: Electronic databases were retrieved to search relevant publications. Pooled standard mean difference (SMD) with 95% confidence interval (CI) was calculated by the random-effect model...
November 2017: International Immunopharmacology
https://www.readbyqxmd.com/read/28983591/chondromodulin%C3%A2-i-suppresses-tumorigenesis-of-human-osteosarcoma-cells
#19
Xiangbo Lin, Lijun Wang, Faming Wang
Osteosarcoma is the most common type of bone cancer, and accounts for ~3% of cancers that occurring in children. Chondromodulin‑I (ChM-I) is a 25 kDa glycoprotein that is expressed mainly in cartilage. ChM-I demonstrates anti‑angiogenic activity and has been suggested to inhibit endothelial cells from invading cartilage, and then has been shown to be an inhibitor of tumorigenesis. However, it remains unclear if ChM‑I has any direct anti‑tumorigenesis role on osteosarcoma. Therefore, the present study aimed to identify whether ChM‑I has any direct inhibit effect on human osteosarcoma cells...
December 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28983302/biofilm-attached-cultivation-of-chlorella-pyrenoidosa-is-a-developed-system-for-swine-wastewater-treatment-and-lipid-production
#20
Pengfei Cheng, Yuanzhu Wang, Tianzhong Liu, Defu Liu
This study showed the new potential of using soluble contents and heavy metals in swine wastewater as nutrient supplements for the algae Chlorella pyrenoidosa with biofilm attached method. Algae with biofilm attached cultivation grew well in unpasteurized wastewater reaching a biomass productivity of 5.03 g m(-2) d(-1), lipid content of 35.9% and lipid productivity of 1.80 g m(-2) d(-1). Chlorella grew in BG11 medium delivered lower values for each of the aforementioned parameters. The FAMEs compositions in the algae paste were mainly consisted of C16:0, C18:2, and C18:3...
2017: Frontiers in Plant Science
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