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https://www.readbyqxmd.com/read/28628981/biodiesel-synthesis-from-calophyllum-inophyllum-oil-with-different-supercritical-fluids
#1
Neha Lamba, Kaveri Gupta, Jayant M Modak, Giridhar Madras
Biodiesel or fatty acid methyl esters (FAMEs) is primarily synthesized using edible vegetable oils and methanol with a catalyst. However, in the present study, FAMEs were synthesized from a non-edible oil (Calophyllum inophyllum also called as sura honne, Punnagam, Alexandrian Laurel) in different supercritical fluids: methanol (MeOH), methyl tert-butyl ether (MTBE), methyl acetate (MeOAc) and dimethyl carbonate (DMC) non-catalytically. Reactions were performed from 523K to 673K at 30MPa with a molar ratio of 40:1 with times varying from 3min to 3h...
June 8, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28624246/uv-c-irradiation-accelerates-neutral-lipid-synthesis-in-the-marine-oleaginous-diatom-fistulifera-solaris
#2
Atsushi Arakaki, Takuya Matsumoto, Takuma Tateishi, Mitsufumi Matsumoto, Daisuke Nojima, Yoshino Tomoko, Tsuyoshi Tanaka
This study investigated the induction of oil synthesis in the oleaginous diatom, Fistulifera solaris, following irradiation with small doses of UV-C. A rapid induction of oil accumulation was confirmed within 6h following UV-C radiation of the diatom cells, with increases in cell oil body volumes after 24h of approximately 4- to 6-fold from the initial volume. Reactive oxygen species (ROS), which can be generated by a UV-C-mediated reaction, were detected in irradiated cells and the correlation between ROS generation and oil accumulation was confirmed...
June 1, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28615918/lung-cancer-pathological-image-analysis-using-a-hidden-potts-model
#3
Qianyun Li, Faliu Yi, Tao Wang, Guanghua Xiao, Faming Liang
Nowadays, many biological data are acquired via images. In this article, we study the pathological images scanned from 205 patients with lung cancer with the goal to find out the relationship between the survival time and the spatial distribution of different types of cells, including lymphocyte, stroma, and tumor cells. Toward this goal, we model the spatial distribution of different types of cells using a modified Potts model for which the parameters represent interactions between different types of cells and estimate the parameters of the Potts model using the double Metropolis-Hastings algorithm...
2017: Cancer Informatics
https://www.readbyqxmd.com/read/28604970/-study-of-the-molecular-basis-for-an-individual-with-bel-variant-due-to-deletion-of-b-glycosyltransferase-gene
#4
Yanling Ying, Xiaozhen Hong, Shu Chen, Xianguo Xu, Kairong Ma, Xiaofei Lan, Ji He, Faming Zhu
OBJECTIVE: To explore the molecular basis of an individual with Bel variant of the ABO blood group. METHODS: The ABO antigen and serum antibody of the individual were detected by serological method. All coding regions and flanking introns of the ABO gene were amplified with PCR and sequenced bidirectionally. The haplotypes of the individual were analyzed by cloning and sequencing. A three dimensional model of the mutant protein was constructed and analyzed. RESULTS: The individual has expressed a very weak B antigen on its red blood cells by absorption and elution testing, which was identified as a Bel variant phenotype...
June 10, 2017: Zhonghua Yi Xue Yi Chuan Xue za Zhi, Zhonghua Yixue Yichuanxue Zazhi, Chinese Journal of Medical Genetics
https://www.readbyqxmd.com/read/28588567/biofilm-vs-planktonic-lifestyle-consequences-for-pesticide-2-4-d-metabolism-by-cupriavidus-necator-jmp134
#5
Thomas Z Lerch, Claire Chenu, Marie F Dignac, Enrique Barriuso, André Mariotti
The development of bacterial biofilms in natural environments may alter important functions, such as pollutant bioremediation by modifying both the degraders' physiology and/or interactions within the matrix. The present study focuses on the influence of biofilm formation on the metabolism of a pesticide, 2,4-dichlorophenoxyacetic acid (2,4-D), by Cupriavidus necator JMP134. Pure cultures were established in a liquid medium with 2,4-D as a sole carbon source with or without sand grains for 10 days. Bacterial numbers and 2,4-D concentrations in solution were followed by spectrophotometry, the respiration rate by gas chromatography and the surface colonization by electron microscopy...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28580860/the-effects-of-social-concern-goals-on-the-value-of-learning-and-on-the-intentions-of-medical-students-to-change-their-majors
#6
Soowon Park, Seunghee Cho, Jun-Young Lee
BACKGROUND: In the process of developing a professional medical expertise, goals can become a psychological impetus and act as a source of retaining an individual's persistency. Therefore, the goals of medical students should be considered when designing a curriculum for health professions. PURPOSE: The purpose of this study was to examine relative effects of goal categories on the value of learning and intention to change one's major. METHOD: Data were obtained from the Korea Education Longitudinal Study, which included 1938 representative Korean college freshmen majoring in medicine, engineering, natural science and humanities...
2017: Medical Education Online
https://www.readbyqxmd.com/read/28571547/potential-of-macroalgae-ulva-lactuca-as-a-source-feedstock-for-biodiesel-production
#7
Hanaa H Abd El Baky, Gamal S El Baroty
BACKGROUND: The aim of this study was to investigate the possibility of growing of algae Ulva lactuca L under different salinity levels coupled with varied KNO3 concentrations (source of N) as a potential source of oil for biodiesel production. METHODS: U. lactuta was cultured in 10.0% NaCl coupled with either 2.5 g/L (S1+ 1N) or 1.0 g/L KNO3 (S1+ 2N) and in 30.0% NaCl coupled with 2.5 g/L (S2+ 1N) or 1.0 g/L KNO3 (S2+ 2N) nutrient medium. Among all algae cultures, biomass (dry weight) and lipid accumulation (total lipid content, TL) were significantly different (P>0...
June 1, 2017: Recent Patents on Food, Nutrition & Agriculture
https://www.readbyqxmd.com/read/28569156/how-chinese-clinicians-face-ethical-and-social-challenges-in-fecal-microbiota-transplantation-a-questionnaire-study
#8
Yonghui Ma, Jinqiu Yang, Bota Cui, Hongzhi Xu, Chuanxing Xiao, Faming Zhang
BACKGROUND: Fecal microbiota transplantation (FMT) is reportedly the most effective therapy for relapsing Clostridium Difficile infection (CDI) and a potential therapeutic option for many diseases. It also poses important ethical concerns. This study is an attempt to assess clinicians' perception and attitudes towards ethical and social challenges raised by fecal microbiota transplantation. METHODS: A questionnaire was developed which consisted of 20 items: four items covered general aspects, nine were about ethical aspects such as informed consent and privacy issues, four concerned social and regulatory issues, and three were about an FMT bank...
May 31, 2017: BMC Medical Ethics
https://www.readbyqxmd.com/read/28541721/a-joint-bayesian-model-for-integrating-microarray-and-rna-sequencing-transcriptomic-data
#9
Tianzhou Ma, Faming Liang, Steffi Oesterreich, George C Tseng
As the sequencing cost continued to drop in the past decade, RNA sequencing (RNA-seq) has replaced microarray to become the standard high-throughput experimental tool to analyze transcriptomic profile. As more and more datasets are generated and accumulated in the public domain, meta-analysis to combine multiple transcriptomic studies to increase statistical power has received increasing popularity. In this article, we propose a Bayesian hierarchical model to jointly integrate microarray and RNA-seq studies...
May 25, 2017: Journal of Computational Biology: a Journal of Computational Molecular Cell Biology
https://www.readbyqxmd.com/read/28521228/acidified-flocculation-process-for-harvesting-of-microalgae-coagulant-reutilization-and-metal-free-microalgae-recovery
#10
Dong-Yeon Kim, Kyubock Lee, Jiye Lee, Young-Hee Lee, Jong-In Han, Ji-Yeon Park, You-Kwan Oh
Chemical flocculation is considered to be an overall low-cost and up-scalable process for harvesting of microalgae. In this study a new flocculation approach utilizing metal coagulant (Fe2(SO4)3) and sulfuric acid (H2SO4) was introduced for harvesting of Chlorella sp. KR-1, which overcome two main issues of contamination and reuse of coagulant. Reduction of pH successfully released precipitates attached to the microalgae, and the remaining acidic solution containing recovered ferric ions could be reused for harvesting up to three times with high, better-than 98% efficiencies...
September 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28505772/fame-and-obsolescence-disentangling-growth-and-aging-dynamics-of-patent-citations
#11
K W Higham, M Governale, A B Jaffe, U Zülicke
We present an analysis of citations accrued over time by patents granted by the United States Patent and Trademark Office in 1998. In contrast to previous studies, a disaggregation by technology category is performed, and exogenously caused citation-number growth is controlled for. Our approach reveals an intrinsic citation rate that clearly separates into an-in the long run, exponentially time-dependent-aging function and a completely time-independent preferential-attachment-type growth kernel. For the general case of such a separable citation rate, we obtain the time-dependent citation distribution analytically in a form that is valid for any functional form of its aging and growth parts...
April 2017: Physical Review. E
https://www.readbyqxmd.com/read/28501730/larvicidal-activity-of-vegetable-oils-and-esterified-compounds-against-culex-quinquefasciatus-diptera-culicidae
#12
José A Ribeiro-Neto, Maria Eduarda A Pinto, Vanessa V Ferreira, Jacqueline D Tibúrcio, Fernando P Varotti, Dihego O Azevedo, Ezequias P Siqueira-Filho, José E Serrão, Luciana A Santos Lima, Stenio Nunes Alves
Control of Culex quinquefasciatus using chemical insecticides may result in the selection of resistant mosquito strains. Thus, the use of plant-derived products has been studied as alternative for the mosquito control. Fatty acid methyl esters (FAMEs) obtained by transesterification of vegetable oils may result in compounds with larvicidal potential against C. quinquefasciatus. However, little is known about the morphological, physiological or biochemical effects that these FAMEs may have on mosquito larvae...
May 11, 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/28501685/lipid-for-biodiesel-production-from-attached-growth-chlorella-vulgaris-biomass-cultivating-in-fluidized-bed-bioreactor-packed-with-polyurethane-foam-material
#13
Ainur-Assyakirin Mohd-Sahib, Jun-Wei Lim, Man-Kee Lam, Yoshimitsu Uemura, Mohamed Hasnain Isa, Chii-Dong Ho, Shamsul Rahman Mohamed Kutty, Chung-Yiin Wong, Siti-Suhailah Rosli
The potential to grow attached microalgae Chlorella vulgaris in fluidized bed bioreactor was materialized in this study, targeting to ease the harvesting process prior to biodiesel production. The proposed thermodynamic mechanism and physical property assessment of various support materials verified polyurethane to be suitable material favouring the spontaneous adhesion by microalgae cells. The 1-L bioreactor packed with only 2.4% (v/v) of 1.00-mL polyurethane foam cubes could achieve the highest attached growth microalgae biomass and lipid weights of 812±122 and 376±37mg, respectively, in comparison with other cube sizes...
September 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28500884/rapid-biodiesel-synthesis-from-waste-pepper-seeds-without-lipid-isolation-step
#14
Jechan Lee, Jieun Kim, Yong Sik Ok, Eilhann E Kwon
In situ transformation of lipid in waste pepper seeds into biodiesel (i.e., fatty acid methyl esters: FAMEs) via thermally-induced transmethylation on silica was mainly investigated in this study. This study reported that waste pepper seeds contained 26.9wt% of lipid and that 94.1% of the total lipid in waste pepper seeds could be converted into biodiesel without lipid extraction step for only ∼1min reaction time. This study also suggested that the optimal temperature for in situ transmethylation was identified as 390°C...
September 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28493913/pathogenic-variants-screening-in-seventeen-candidate-genes-on-2p15-for-association-with-ankylosing-spondylitis-in-a-han-chinese-population
#15
Mengmeng Wang, Lihong Xin, Guoqi Cai, Xu Zhang, Xiao Yang, Xiaona Li, Qing Xia, Li Wang, Shengqian Xu, Jianhua Xu, Zongwen Shuai, Changhai Ding, Faming Pan
OBJECTIVES: Previous studies have found the association between rs10865331 in 2p15 area and ankylosing spondylitis (AS). This study aimed to identify additional functional genetic variants in 2p15 region associated with AS susceptibility. METHODS: We used next generation sequencing (NGS) in 100 AS cases and 100 healthy controls to screen AS susceptible genetic variants, and validated these variants in 620 cases and 620 controls by using imLDRTM technique for single nucleotide polymorphism (SNP) genotyping...
2017: PloS One
https://www.readbyqxmd.com/read/28493452/suitability-of-selected-chromatographic-columns-for-analysis-of-fatty-acids-in-dialyzed-patients
#16
Magdalena Pazda, Piotr Stepnowski, Tomasz Sledzinski, Michal Chmielewski, Adriana Mika
Gas chromatography-mass spectrometry is a preferred method for fatty acid (FA) analysis in biofluids from patients with metabolic diseases. Complex characteristics of FAs make their analysis particularly challenging. Selection of an appropriate chromatographic column is particularly important component of the process as it provides optimal separation and detection of possibly all FAs present in the sample. However, no accurate protocol for comparative evaluation of capillary columns for the analysis of whole serum FA profile in patients with chronic kidney disease (CKD) has been developed thus far...
May 10, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/28479721/fatty-acid-profiling-and-in-vitro-antihyperglycemic-effect-of-leucas-cephalotes-roth-spreng-via-carbohydrate-hydrolyzing-enzyme-inhibition
#17
Anjali Verma, Anil Kumar, Dalip Kumar Upreti, Veena Pande, Mahesh Pal
BACKGROUND: Leucas cephalotes has been used by many tribes to treat variety of diseases and known to have many essential secondary metabolites. To the best of our knowledge, it is the first comparative analysis of total fatty acid (FA) composition and α-amylase inhibition activity of L. cephalotes. OBJECTIVE: The present study is carried out to explore the antihyperglycemic activity and FA contents of all parts of L. cephalotes. MATERIAL AND METHOD: Fruits, leaves, stems, and roots part of L...
January 2017: Pharmacognosy Magazine
https://www.readbyqxmd.com/read/28469387/integrative-analysis-of-gene-networks-and-their-application-to-lung-adenocarcinoma-studies
#18
Sangin Lee, Faming Liang, Ling Cai, Guanghua Xiao
The construction of gene regulatory networks (GRNs) is an essential component of biomedical research to determine disease mechanisms and identify treatment targets. Gaussian graphical models (GGMs) have been widely used for constructing GRNs by inferring conditional dependence among a set of gene expressions. In practice, GRNs obtained by the analysis of a single data set may not be reliable due to sample limitations. Therefore, it is important to integrate multiple data sets from comparable studies to improve the construction of a GRN...
2017: Cancer Informatics
https://www.readbyqxmd.com/read/28438358/potential-of-oleaginous-yeast-trichosporon-sp-for-conversion-of-sugarcane-bagasse-hydrolysate-into-biodiesel
#19
K K Brar, A K Sarma, Mohammad Aslam, Igor Polikarpov, B S Chadha
This study reports production of microbial oil from a yeast strain Trichosporon sp., (RW) isolated from decayed wood. Preliminary analysis based on fluorescence microscopy and spectroscopy of Nile red stained yeast cells showed accumulation of lipid globules. The potential of the yeast to produce lipids was evaluated on glucose, glycerol and acid hydrolysate of sugarcane bagasse, where Trichosporon sp. (RW) was found to accumulate 21.45 (59.6%), 18.41 (56%) and 10.25g/l (40.5%) of the lipids after 120h of fermentation at 30°C...
March 28, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28433903/one-step-production-of-biodiesel-from-wet-and-unbroken-microalgae-biomass-using-deep-eutectic-solvent
#20
Ying Pan, Md Asraful Alam, Zhongming Wang, Dalong Huang, Keqin Hu, Hongxuan Chen, Zhenhong Yuan
One-step and Two-step methods were studied for lipid extraction from wet and unbroken (water content is 65-67%) Chlorella sp. and Chlorococcum sp. (GN38) using deep eutectic solvent (DES) treated microalgae cells. Further we optimized the extraction process and studied on its underlying mechanism. Among all DES, Choline chloride-Acetic acid (Ch-Aa) DES treatment showed optimal conditions at the mass ratio of DES: methanol-H2SO4 (2.00%) mixture: algae biomass was 60:40:3 with reaction time was 60min, and the optimum temperature was 110°C (Chlorococcum sp...
August 2017: Bioresource Technology
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