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https://www.readbyqxmd.com/read/28941404/development-of-a-simple-multi-residue-determination-method-of-80-veterinary-drugs-in-oplegnathus-punctatus-by-liquid-chromatography-coupled-to-quadrupole-orbitrap-mass-spectrometry
#1
Fei Zhao, Xin Gao, Zhixu Tang, Xin Luo, Miaomiao Wu, Jiachao Xu, Xiaoting Fu
A simple, rapid and sensitive multi-residue analytical method was developed and validated for 80 veterinary drugs in Oplegnathus punctatus using ultrahigh performance liquid chromatography-Orbitrap high resolution mass spectrometry (LC-HRMS). The analytes belong to 12 different families include benzimidazoles, β-lactams, lincosamides, macrolides, nitromidazoles, quinolones, sulfonamides and trimethoprim, tetracyclines, triphenylmethane dyes, amphenicols, nonsteroidal estrogens and steroid hormones. The sample preparation was optimized base on QuEChERS (quick, easy, cheap, effective, rugged and safe) procedure...
September 18, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28938774/evaluation-of-encapsulated-sodium-butyrate-on-growth-performance-energy-digestibility-gut-development-and-salmonella-colonization-in-broilers
#2
J D Liu, H O Bayir, D E Cosby, N A Cox, S M Williams, J Fowler
Two experiments were conducted to determine the effect of an encapsulated sodium butyrate (Na-B) with targeted releasing times on broiler performance, energy digestibility, intestinal morphology, and ceca Salmonella colonization. In experiment 1, 3 different Na-B products (CMA, CMP, and CMS) were evaluated following a challenge with a nalidixic acid-resistant Salmonella typhimurium (STNAR). Cobb-Cobb male birds were placed 8 per pen into 6 replicates for each treatment. Treatments included 6 Na-B treatments (500 and 1,000 ppm of each product) plus 2 control (non-challenged and challenged)...
October 1, 2017: Poultry Science
https://www.readbyqxmd.com/read/28938751/new-permeable-cryoprotectant-free-vitrification-method-for-native-human-sperm
#3
J Aizpurua, L Medrano, M Enciso, J Sarasa, A Romero, M A Fernández, M J Gómez-Torres
STUDY QUESTION: Is permeable cryoprotectant-free vitrification of native sperm samples a good alternative to conventional slow freezing? SUMMARY ANSWER: The permeable cryoprotectant-free sperm vitrification protocol tested in this study renders considerably better recovery rates of good quality sperm compared to slow freezing. WHAT IS KNOWN ALREADY: Slow freezing is currently the most commonly used technique for sperm cryopreservation, though this method has been repeatedly shown to have negative effects on both structural and functional sperm features...
October 1, 2017: Human Reproduction
https://www.readbyqxmd.com/read/28938625/predictive-value-of-preoperative-retinal-nerve-fiber-layer-thickness-for-postoperative-visual-recovery-in-patients-with-chiasmal-compression
#4
Jing Zhang, Sunfu Zhang, Yanlin Song, Chenjing Zhu, Min He, Qingqing Ren, Baoyin Shan, Ziqiong Wang, Yunhui Zeng, Jianguo Xu
The aim of this study was to evaluate the predictive role of preoperative retinal nerve fiber layer (RNFL) thickness for postoperative visual recovery in patients with chiasmal compression through performing a meta-analysis. PubMed, EMBASE, Cochrane Library and China National Knowledge Infrastructure were searched for relevant studies. The study and patient characteristics were extracted. Pooled odds ratio (OR) with 95% confidence interval (CI) was calculated to estimate the predictive value of RNFL thickness...
August 29, 2017: Oncotarget
https://www.readbyqxmd.com/read/28938612/magnetic-resonance-imaging-tracking-and-assessing-repair-function-of-the-bone-marrow-mesenchymal-stem-cells-transplantation-in-a-rat-model-of-spinal-cord-injury
#5
Hongwu Zhang, Liqin Wang, Shihong Wen, Qingfeng Xiang, Xianhong Xiang, Caixia Xu, Yong Wan, Jingnan Wang, Bin Li, Yiqian Wan, Zhiyun Yang, David Y B Deng
The transplantation of bone marrow mesenchymal stem cells (BMSCs) to repair spinal cord injury (SCI) has become a promising therapy. However, there is still a lack of visual evidence directly implicating the transplanted cells as the source of the improvement of spinal cord function. In this study, BMSCs were labeled with NF-200 promoter and lipase-activated gadolinium-containing nanoparticles (Gd-DTPA-FA). Double labeled BMSCs were implanted into spinal cord transaction injury in rat models in situ, the function recovery was evaluated on 1st, 7th, 14th, 28 th days by MRI, Diffusion Tensor Imaing, CT imaging and post-processing, and histological observations...
August 29, 2017: Oncotarget
https://www.readbyqxmd.com/read/28938363/reconstruction-of-the-heel-middle-foot-sole-and-plantar-forefoot-with-the-medial-plantar-artery-perforator-mpap-flap-clinical-experience-with-28-cases
#6
Mario F Scaglioni, Daniel Rittirsch, Pietro Giovanoli
INTRODUCTION: Defects in the weight-bearing region of the foot sole can represent a substantial restriction in quality of life and pose a challenge for reconstructive plastic surgery.The purpose of this article is to report our experience with the use of medial plantar artery perforator (MPAP) flap for reconstruction of defects of the foot sole in 3 different regions: heel, middle foot sole and plantar forefoot. PATIENTS AND METHODS: From January 2003 to May 2016, 28 patients (13 males and 15 females) with an average age of 54 years (range 12 to 84 y...
September 15, 2017: Plastic and Reconstructive Surgery
https://www.readbyqxmd.com/read/28938362/muscle-versus-fasciocutaneous-free-flaps-in-lower-extremity-traumatic-reconstruction-a-multicenter-outcomes-analysis
#7
Eugenia H Cho, Ronnie L Shammas, Martin J Carney, Jason M Weissler, Andrew R Bauder, Adam D Glener, Stephen J Kovach, Scott T Hollenbeck, L Scott Levin
PURPOSE: Clinical indications are expanding for the use of fasciocutaneous free flaps in lower extremity traumatic reconstruction. We assessed the impact of muscle versus fasciocutaneous free flap coverage on reconstructive and functional outcomes. METHODS: A multicenter retrospective review was conducted on all lower extremity traumatic free flaps performed at Duke University (1997-2013) and the University of Pennsylvania (2002-2013). Muscle and fasciocutaneous flaps were compared in two subgroups (acute trauma and chronic traumatic sequelae), according to limb salvage, return to ambulation, flap outcomes, and secondary flap procedures...
September 15, 2017: Plastic and Reconstructive Surgery
https://www.readbyqxmd.com/read/28938332/mandibular-condyle-reconstruction-with-fibula-free-tissue-transfer-the-role-of-the-masseter-muscle
#8
Andreas Gravvanis, Dimitrios Anterriotis, Despoina Kakagia
BACKGROUND: Free fibula flap is an option for primary restoration after disarticulation mandibular resection, though literature on technique refinements is scarce. The authors hypothesized that inset of the masseter, the key mandibular elevator muscle, at the reconstructed mandible may optimize functional recovery. METHODS: All patients undergoing reconstruction of mandibulectomy-condylectomy defect (January 2009 to January 2014) by means of a fibular flap were prospectively studied...
September 21, 2017: Journal of Craniofacial Surgery
https://www.readbyqxmd.com/read/28938300/enhancing-the-quality-and-safety-of-the-perioperative-patient
#9
Sven Staender, Andrew Smith
PURPOSE OF REVIEW: Many possible hazards bedevil the perioperative patient. This review focuses on a number of aspects of perioperative management where the patient's quality and safety can be enhanced. RECENT FINDINGS: Our understanding of the relationship between preoperative preparation and postoperative outcomes has improved. There have also been recent developments in our understanding of how to construct useful cognitive aids and make the best use of checklists by understanding the cultural environment supporting their use...
September 21, 2017: Current Opinion in Anaesthesiology
https://www.readbyqxmd.com/read/28938275/inpatient-treatment-of-patients-admitted-for-dizziness-a-population-based-healthcare-research-study-on-epidemiology-diagnosis-treatment-and-outcome
#10
Vera Renner, Katharina Geißler, Daniel Boeger, Jens Buentzel, Dirk Esser, Kerstin Hoffmann, Peter Jecker, Andreas Mueller, Gerald Radtke, Hubertus Axer, Orlando Guntinas-Lichius
OBJECTIVE: To determine inpatient treatment rates of patients with dizziness with focus on diagnostics, treatment and outcome. STUDY DESIGN: Retrospective population-based study. SETTING: Inpatients in the federal state Thuringia in 2014. PATIENTS: All 1,262 inpatients (62% females, median age: 61 yr) treated for inpatient dizziness were included. MAIN OUTCOME MEASURES: The association between analyzed parameters and probability of improvement and recovery was tested using univariable and multivariable statistics...
September 21, 2017: Otology & Neurotology
https://www.readbyqxmd.com/read/28938199/determination-of-lead-at-trace-levels-in-mussel-and-sea-water-samples-using-vortex-assisted-dispersive-liquid-liquid-microextraction-slotted-quartz-tube-flame-atomic-absorption-spectrometry
#11
Sezin Erarpat, Gözde Özzeybek, Dotse Selali Chormey, Sezgin Bakırdere
In this study, dispersive liquid-liquid microextraction (DLLME) and slotted quartz tube (SQT) were coupled to flame atomic absorption spectrometry (FAAS) to increase the sensitivity of lead. Conditions such as the formation of the lead-dithizone complex, efficiency of the DLLME method and the output of the SQT were systematically optimized to improve the detection limit for the analyte. The conventional FAAS system was improved upon by about 3.0 times with SQT-FAAS, 32 times with DLLME-FAAS and 142 times with DLLME-SQT-FAAS...
September 17, 2017: Chemosphere
https://www.readbyqxmd.com/read/28938132/development-and-validation-of-carbofuran-and-3-hydroxycarbofuran-analysis-by-high-pressure-liquid-chromatography-with-diode-array-detector-hplc-dad-for-forensic-veterinary-medicine
#12
Vagner Gonçalves, Nicolle Queiroz Hazarbassanov, Adriana de Siqueira, Jorge Camilo Florio, Claudia Helena Pastor Ciscato, Paulo Cesar Maiorka, Andre Fukushima, Helenice de Souza Spinosa
Agricultural pesticides used with the criminal intent to intoxicate domestic and wild animals are a serious concern in Veterinary Medicine. In order to identify the pesticide carbofuran and its metabolite 3- hydroxycarbofuran in animals suspected of exogenous intoxication a high pressure liquid chromatography with diode array detector (HPLC-DAD) method was developed and validated in stomach contents, liver, vitreous humor and blood. The method was evaluated using biological samples from seven different animal species...
September 14, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28938108/cervical-arthroplasty-versus-anterior-cervical-fusion-for-symptomatic-adjacent-segment-disease-after-anterior-cervical-fusion-surgery-review-of-treatment-in-41-patients
#13
Sang-Bok Lee, Kyoung-Suok Cho
OBJECTIVE: The purpose of this study is to compare the efficacy and safety of anterior cervical discectomy and fusion (ACDF) and cervical total disc replacement (CTDR) as revision surgeries for symptomatic adjacent segment degeneration (ASD) in cases with previous ACDF. PATIENTS AND METHODS: Between 2010 and 2014, 41 patients with previous cervical fusion surgery underwent ACDF or CTDR for symptomatic ASD. Twenty-two patients in the ACDF group underwent 26 ACDFs, and 19 patients in the CTDR group underwent 25 arthroplasties for symptomatic ASD...
August 4, 2017: Clinical Neurology and Neurosurgery
https://www.readbyqxmd.com/read/28937981/cognitive-function-in-patients-with-stable-coronary-heart-disease-related-cerebrovascular-and-cardiovascular-responses
#14
Mathieu Gayda, Vincent Gremeaux, Louis Bherer, Martin Juneau, Joffrey Drigny, Olivier Dupuy, Gabriel Lapierre, Véronique Labelle, Annik Fortier, Anil Nigam
Chronic exercise has been shown to prevent or slow age-related decline in cognitive functions in otherwise healthy, asymptomatic individuals. We sought to assess cognitive function in a stable coronary heart disease (CHD) sample and its relationship to cerebral oxygenation-perfusion, cardiac hemodynamic responses, and [Formula: see text] peak compared to age-matched and young healthy control subjects. Twenty-two young healthy controls (YHC), 20 age-matched old healthy controls (OHC) and 25 patients with stable CHD were recruited...
2017: PloS One
https://www.readbyqxmd.com/read/28937936/is-high-t-1-slope-a-significant-risk-factor-for-developing-interlaminar-bony-fusion-after-cervical-laminoplasty-a-retrospective-cohort-study
#15
Takeshi Oichi, Yasushi Oshima, Hiroyuki Oka, Yuki Taniguchi, Hirotaka Chikuda, Yoshitaka Matsubayashi, Katsushi Takeshita, Sakae Tanaka
OBJECTIVE Several investigators have reported the occurrence of interlaminar bony fusion after cervical laminoplasty, which is reportedly associated with reduced postoperative cervical range of motion (ROM). However, to the authors' knowledge, no previous study has investigated the characteristics of patients who were likely to develop interlaminar bony fusion after cervical laminoplasty. Therefore, the objective of this study was to investigate the risk factors for interlaminar bony fusion in patients with cervical spondylotic myelopathy (CSM) following cervical laminoplasty and to investigate the effect of interlaminar bony fusion on surgical outcomes...
September 15, 2017: Journal of Neurosurgery. Spine
https://www.readbyqxmd.com/read/28937911/characterization-and-application-of-the-enzyme-peroxidase-to-the-degradation-of-the-mycotoxin-don
#16
Ana Carla P Feltrin, Sabrina De O Garcia, Sergiane S Caldas, Ednei G Primel, Eliana Badiale-Furlong, Jaqueline Garda-Buffon
Deoxynivalenol (DON), one of the main mycotoxins found in food matrices, has high level of toxicity. This study aimed to characterize the peroxidase enzyme extracted from rice bran to be applied to the biodegradation of DON in order to evaluate the potential peroxidase (PO) from rice bran (RB) has to degrade DON in optimal conditions. Purification and recovery factors of PO extracted from RB and purified by three-phase partitioning were 5.7% and 50%, respectively. PO had the highest level of activity in the phosphate buffer 5 mM pH 5...
September 22, 2017: Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes
https://www.readbyqxmd.com/read/28937880/detection-of-free-microcystins-in-the-liver-and-muscle-of-freshwater-fish-by-liquid-chromatography-tandem-mass-spectrometry
#17
Xiaobin Hu, Jinyun Ye, Rongfei Zhang, Xiang Wu, Yixiang Zhang, Chenglong Wu
MC analysis of biological tissue is considered to be very difficult due to the lack of validated methods. This is the primary limiting factor for monitoring potential risks in both the flesh of aquatic organisms and the aquatic ecosystem. In this study, an effective method to determine free MCs (MC-LR and MC-RR) in the muscle and liver tissues of freshwater cultured fish was developed using solid-phase extraction (SPE) and liquid chromatography-tandem mass spectrometry (LC/MS-MS). The extraction solvent, time of extraction, eluent and purification of the extract were optimized...
September 22, 2017: Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes
https://www.readbyqxmd.com/read/28937864/determination-of-seven-pyrethroid-pesticides-residue-in-vegetables-by-gas-chromatography-using-carboxylated-multi-walled-carbon-nanotubes-as-dispersion-solid-phase-extraction-sorbent
#18
Peng Sun, Yuling Gao, Chong Xu, Yongfu Lian
:A novel carboxylated multi-walled carbon nanotubes (MWCNTs-COOH) dispersive solid phase extraction (d-SPE) method combined with gas chromatography (GC) with ECD detection was developed for the determination of seven pyrethroid pesticides in cucumber, spinach, eggplant, tomato and carrot. We optimized d-SPE conditions including the type and volume of the extractant, the type and amount of the sorbent, and shaking time. Under the optimal conditions, the linear range was from 2.0 to 2000 μg kg(-1). The recoveries were from 88...
September 22, 2017: Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment
https://www.readbyqxmd.com/read/28937862/the-effect-of-preoperative-oral-carbohydrate-on-breastfeeding-after-cesarean-section-a-double-blind-randomized-controlled-clinical-trial
#19
Razieh Khalooei Fard, Zohre Tabassi, Mostafa Qorbani, Saeed Hosseini
Delay in the initiation of breastfeeding is one of the problems of cesarean section. Its causes are insulin resistance, pain, anxiety, stress, thirst, hunger, and so on. Preoperative oral carbohydrate (OCH) reduces postoperative insulin resistance and improves postoperative recovery. The present study was conducted to evaluate the effect of preoperative oral carbohydrate on breastfeeding after cesarean section. In this double-blind randomized clinical trial, 91 pregnant women who underwent elective cesarean section were randomly assigned to preoperative OCH (Nutricia Preop; n = 45) or control group (water flavored with lemon; n = 46)...
September 22, 2017: Journal of Dietary Supplements
https://www.readbyqxmd.com/read/28937789/identifying-dyslexia-risk-in-student-athletes-a-preliminary-protocol-for-concussion-management
#20
Rebecca Wiseheart, Robin Wellington
Learning disability (LD) has been identified as a potential risk factor for protracted recovery from a sport-related concussion, yet students with LD are rarely included in concussion research. Here, we draw special attention to dyslexia, a common but often underdiagnosed LD. Reading and learning problems commonly associated with dyslexia are often masked by protective factors, such as high verbal ability or general intelligence. Hence, high-achieving individuals with dyslexia may not be identified as being in a high-risk category...
September 22, 2017: Journal of Athletic Training
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