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https://www.readbyqxmd.com/read/28646652/influenza-a-virus-cap-snatches-host-rnas-based-on-their-abundance-early-after-infection
#1
Dorota Sikora, Lynda Rocheleau, Earl G Brown, Martin Pelchat
The influenza A virus RNA polymerase cleaves the 5' ends of host RNAs and uses these RNA fragments as primers for viral mRNA synthesis. We performed deep sequencing of the 5' host-derived ends of the eight viral mRNAs of influenza A/Puerto Rico/8/1934 (H1N1) virus in infected A549 cells, and compared the population to those of A/Hong Kong/1/1968 (H3N2) and A/WSN/1933 (H1N1). In the three strains, the viral RNAs target different populations of host RNAs. Host RNAs are cap-snatched based on their abundance, and we found that RNAs encoding proteins involved in metabolism are overrepresented in the cap-snatched populations...
June 21, 2017: Virology
https://www.readbyqxmd.com/read/28640800/hiv-testing-linkage-to-hiv-medical-care-and-interviews-for-partner-services-among-youths-61-health-department-jurisdictions-united-states-puerto-rico-and-the-u-s-virgin-islands-2015
#2
Renee Stein, Wei Song, Mariette Marano, Heta Patel, Shubha Rao, Elana Morris
Identifying persons living with human immunodeficiency virus (HIV) who are unaware of their infection, linking them to HIV medical care, and reducing health disparities are important national goals (1). Of the 8,841 teens and young adults aged 13-24 years (collectively referred to as youths in this report) who received a diagnosis of HIV in 2014, 70% were young men who have sex with men (MSM) (2). In the same year, an estimated 52% of young MSM living with HIV were unaware of their infection compared with 15% among all persons living with HIV (3)...
June 23, 2017: MMWR. Morbidity and Mortality Weekly Report
https://www.readbyqxmd.com/read/28639802/a-cross-cultural-analysis-of-the-test-of-memory-malingering-among-latin-american-spanish-speaking-adults
#3
Alicia Nijdam-Jones, Diego Rivera, Barry Rosenfeld, Juan Carlos Arango-Lasprilla
Cognitive efforts tests, such as the Test of Memory Malingering (TOMM; Tombaugh, 1997), are widely used internationally, yet there is a dearth of research that has assessed the utility of these measures in different cultures, countries, and languages. This study evaluated the specificity of the TOMM Trial 2 among a sample of 3,590 Spanish-speaking adults residing in 8 Latin American countries (Argentina, Bolivia, Chile, Colombia, Mexico, Paraguay, Peru, and Puerto Rico). Trial 2 TOMM scores were negatively associated with participants' age and positively associated with level of education...
June 22, 2017: Law and Human Behavior
https://www.readbyqxmd.com/read/28638342/warfarin-anticoagulation-therapy-in-caribbean-hispanics-of-puerto-rico-a-candidate-gene-association-study
#4
Karla Claudio-Campos, Aurora Labastida, Alga Ramos, Andrea Gaedigk, Jessicca Renta-Torres, Dariana Padilla, Giselle Rivera-Miranda, Stuart A Scott, Gualberto Ruaño, Carmen L Cadilla, Jorge Duconge-Soler
Existing algorithms account for ~50% of observed variance in warfarin dose requirements after including common polymorphisms. However, they do not perform as well in populations other than Caucasians, in part because some ethno-specific genetic variants are overlooked. The objective of the present study was to identify genetic polymorphisms that can explain variability in warfarin dose requirements among Caribbean Hispanics of Puerto Rico. Next-Generation Sequencing of candidate genes CYP2C9 and VKORC1 and genotyping by DMET® Plus Assay of cardiovascular patients were performed...
2017: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/28629724/in%C3%A2-vitro-antiviral-effects-of-peganum-harmala-seed-extract-and-its-total-alkaloids-against-influenza-virus
#5
Mohammad-Taghi Moradi, Ali Karimi, Mahmoud Rafieian-Kopaei, Fatemeh Fotouhi
This research was aimed to evaluate the in vitro antiviral effect and the mechanism of the effect of Peganum. harmala seeds extract against influenza A virus infection using Madin-Darby canine kidney (MDCK) cells. In this research, ethyl alcohol extract of P. harmala seeds and its total alkaloids was prepared. The potential antiviral activity of the extract and its total alkaloids against influenza A/Puerto Rico/8/34 (H1N1; PR8) virus was assessed. The mode of action of the extract to inhibit influenza replication was investigated using virucidal activity, hemagglutination inhibition assay, time of addition assays, RNA replication, western blot analysis and RNA polymerase blocking assay...
June 16, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28622406/building-bridges-to-address-health-disparities-in-puerto-rico-the-salud-para-pi%C3%A3-ones-project
#6
Enid J García-Rivera, Princess Pacheco, Marielis Colón, Mary Helen Mays, Maricruz Rivera, Verónica Munet-Díaz, María Del R González, María Rodríguez, Rebecca Rodríguez, Astrid Morales
Over the past several decades, Puerto Ricans have faced increased health threats from chronic diseases, particularly diabetes and hypertension. The patient-provider relationship is the main platform for individual disease management, whereas the community, as an agent of change for the community's health status, has been limited in its support of individual health. Likewise, traditional research approaches within communities have placed academic researchers at the center of the process, considering their knowledge was of greater value than that of the community...
June 2017: Puerto Rico Health Sciences Journal
https://www.readbyqxmd.com/read/28622403/using-network-sampling-and-recruitment-data-to-understand-social-structures-related-to-community-health-in-a-population-of-people-who-inject-drugs-in-rural-puerto-rico
#7
Mayra Coronado-García, Courtney R Thrash, Melissa Welch-Lazoritz, Robin Gauthier, Juan Carlos Reyes, Bilal Khan, Kirk Dombrowski
OBJECTIVE: This research examined the social network and recruitment patterns of a sample of people who inject drugs (PWIDs) in rural Puerto Rico, in an attempt to uncover systematic clustering and between-group social boundaries that potentially influence disease spread. METHODS: Respondent driven sampling was utilized to obtain a sample of PWID in rural Puerto Rico. Through eight initial "seeds", 317 injection drug users were recruited. Using recruitment patterns of this sample, estimates of homophily and affiliation were calculated using RDSAT...
June 2017: Puerto Rico Health Sciences Journal
https://www.readbyqxmd.com/read/28622402/prevalence-of-concurrent-prescribing-of-ace-is-and-arbs-among-beneficiaries-of-puerto-rico-s-government-sponsored-health-care-plan-during-2012-and-2013
#8
Denise Figueroa-Ríos, José J Hernández-Muñoz, Aileen García-Albarrán, Emily A García-Rodríguez, Glendaliz Méndez-Hernández, Suzette M Vélez-Rivera
OBJECTIVE: Cardiovascular conditions are the second cause of death in Puerto Rico. The individual use of angiotensin-converting enzyme inhibitors (ACE-Is) or angiotensin receptor blockers (ARBs) is considered the first-line therapy for the treatment of several cardiovascular-related medical conditions. However, the concurrent use of these 2 therapeutic classes of drugs is not supported by treatment guidelines. Studies have shown that their concurrent use represents a potential health risk...
June 2017: Puerto Rico Health Sciences Journal
https://www.readbyqxmd.com/read/28622401/impact-of-a-pharmacist-conducted-educational-program-on-human-papilloma-virus-vaccination-rates-in-a-low-socioeconomic-population-in-the-city-of-lares-pr
#9
Edda Marie Jiménez-Quiñones, Kyle Melin, Francisco Javier Jiménez-Ramírez
OBJECTIVE: To observe whether local vaccination rates are improved by a patient and physician education program on the human papillomavirus (HPV) vaccine in Farmacia San José, Lares. According to the Puerto Rico Immunization Registry, the HPV vaccine is currently underutilized. METHODS: Patients within the ages of 18 and 26 years who are current patients of Farmacia San José were contacted by phone and approached in person at the pharmacy. Once contacted, patients were provided with educational materials and counseling regarding the risks and benefits of HPV vaccination...
June 2017: Puerto Rico Health Sciences Journal
https://www.readbyqxmd.com/read/28622400/prevalence-of-malocclusion-and-distribution-of-occlusal-characteristics-in-13-to-18-year-old-adolescents-attending-selected-high-schools-in-the-municipality-of-san-juan-pr-2012-2013
#10
Karla Alvarado, Lydia López, Rosana Hanke, Francis Picón, Sona Rivas-Tumanyan
OBJECTIVE: A cross-sectional study was conducted (2012-2013) to evaluate the prevalence of 1) malocclusion and 2) occlusal characteristics in 13- to 18-yearold adolescents in San Juan, Puerto Rico (PR) from selected high schools in the municipality of San Juan. METHODS: The study sample consisted of 155 adolescents. The University of Puerto Rico's IRB approved the study and consent and assent were completed for or by each participant, as applicable. A calibrated dentist performed a single-visit dental evaluation at each school...
June 2017: Puerto Rico Health Sciences Journal
https://www.readbyqxmd.com/read/28622399/comparative-longterm-mortality-trends-in-cancer-vs-ischemic-heart-disease-in-puerto-rico
#11
David Torres, Luis R Pericchi, Hernando Mattei, Juan C Zevallos
OBJECTIVE: Although contemporary mortality data are important for health assessment and planning purposes, their availability lag several years. Statistical projection techniques can be employed to obtain current estimates. This study aimed to assess annual trends of mortality in Puerto Rico due to cancer and Ischemic Heart Disease (IHD), and to predict shorterm and longterm cancer and IHD mortality figures. METHODS: Age-adjusted mortality per 100,000 population projections with a 50% interval probability were calculated utilizing a Bayesian statistical approach of Age-Period-Cohort dynamic model...
June 2017: Puerto Rico Health Sciences Journal
https://www.readbyqxmd.com/read/28616368/correction-insights-of-the-dental-calculi-microbiome-of-pre-columbian-inhabitants-from-puerto-rico
#12
Tasha M Santiago-Rodriguez, Yvonne Narganes-Storde, Luis Chanlatte-Baik, Gary A Toranzos, Raul J Cano
[This corrects the article DOI: 10.7717/peerj.3277.].
2017: PeerJ
https://www.readbyqxmd.com/read/28610279/redescription-of-i-cletocamptus-i-albuquerquensis-and-i-c-dominicanus-i-harpacticoida-canthocamptidae-i-incertae-i-i-sedis-i-and-description-of-two-new-species-from-the-us-virgin-islands-and-bonaire
#13
Samuel Gómez, Ray Gerber, Juan Manuel Fuentes-Reinés
The history surrounding the identity of Cletocamptus albuquerquensis (Herrick, 1894) and C. dominicanus Kiefer, 1934 is very complex. This complexity has been exacerbated by incomplete, and in some cases erroneous, original descriptions of these two species. Also, new records from other locations did not describe the significant characters needed to clearly delineate them. This led several authors to consider C. dominicanus as a synonym of C. albuquerquensis, among other taxonomical considerations regarding, for example, the status of Marshia brevicaudata Herrick, 1894...
May 30, 2017: Zootaxa
https://www.readbyqxmd.com/read/28600275/interactive-voice-response-system-data-considerations-and-lessons-learned-during-a-rectal-microbicide-placebo-adherence-trial-for-young-men-who-have-sex-with-men
#14
Jose Bauermeister, Rebecca Giguere, Cheng-Shiun Leu, Irma Febo, Ross Cranston, Kenneth Mayer, Alex Carballo-Diéguez
BACKGROUND: Rectal microbicides, if proven effective, may aid in reducing human immunodeficiency virus (HIV) incidence; however, demonstration of efficacy and effectiveness is contingent on accurate measurement of product adherence. Delays in self-report, in particular, may affect the accuracy of behavioral data. OBJECTIVE: The aim of this study was to capitalize on mobile phone use by young men who have sex with men (YMSM), and examine the use of an interactive voice response system (IVRS) by YMSM aged 18-30 years enrolled in a multisite, 12-week microbicide safety and acceptability trial...
June 9, 2017: Journal of Medical Internet Research
https://www.readbyqxmd.com/read/28599584/aging-in-puerto-rico-a-comparison-of-health-status-among-island-puerto-rican-and-mainland-u-s-older-adults
#15
Catherine Pérez, Jennifer A Ailshire
OBJECTIVE: To characterize the health status of older island Puerto Ricans, a segment of the U.S. population that has been largely overlooked in aging research. METHOD: Data from the 2002 Puerto Rican Elderly Health Conditions Project and the 2002 Health and Retirement Study are used to examine differences in disease, disability, and self-rated health among island Puerto Ricans and the mainland U.S.-born older adult population. Differences are further examined by gender...
June 1, 2017: Journal of Aging and Health
https://www.readbyqxmd.com/read/28594787/measures-taken-to-prevent-zika-virus-infection-during-pregnancy-puerto-rico-2016
#16
Denise V D'Angelo, Beatriz Salvesen von Essen, Mark J Lamias, Holly Shulman, Wanda I Hernandez-Virella, Aspy J Taraporewalla, Manuel I Vargas, Leslie Harrison, Sascha R Ellington, Leslianne Soto, Tanya Williams, Aurea Rodriguez, Carrie K Shapiro-Mendoza, Brenda Rivera, Shanna Cox, Karen Pazol, Marion E Rice, Deborah L Dee, Lisa Romero, Eva Lathrop, Wanda Barfield, Ruben A Smith, Denise J Jamieson, Margaret A Honein, Carmen Deseda, Lee Warner
Zika virus infection during pregnancy remains a serious health threat in Puerto Rico. Infection during pregnancy can cause microcephaly, brain abnormalities, and other severe birth defects (1). From January 1, 2016 through March 29, 2017, Puerto Rico reported approximately 3,300 pregnant women with laboratory evidence of possible Zika virus infection (2). There is currently no vaccine or intervention to prevent the adverse effects of Zika virus infection during pregnancy; therefore, prevention has been the focus of public health activities, especially for pregnant women (3)...
June 9, 2017: MMWR. Morbidity and Mortality Weekly Report
https://www.readbyqxmd.com/read/28594235/live-attenuated-auxotrophic-mutant-of-salmonella-typhimurium-expressing-immunogenic-ha1-protein-enhances-immunity-and-protective-efficacy-against-h1n1-influenza-virus-infection
#17
Nitin Machindra Kamble, Kim Je Hyoung, John Hwa Lee
AIM: To evaluate the efficacy of attenuated Salmonella typhimurium (JOL912) as a live bacterial vaccine vector. MATERIALS & METHODS: The JOL912 engineered to deliver HA1 protein from influenza A/Puerto Rico/8/1934 (H1N1; PR8) virus was coined as JOL1635 and further evaluated for immunogenicity and protective efficacy. RESULTS: The JOL1635 stably harbored the HA1 gene within pMMP65 plasmid with periplasmic expression and effective delivery of HA1 protein to RAW264...
June 8, 2017: Future Microbiology
https://www.readbyqxmd.com/read/28591685/identification-of-a-consistent-polyene-component-of-purple-pigment-in-diseased-sclerites-of-caribbean-corals-across-region-species-and-insult-agent
#18
Monty L Fetterolf, Chad L Leverette, Christopher Perez, Garriet W Smith
Gorgonians respond to insult (damage and disease) by producing sclerites containing a purple pigment as opposed to the normal white sclerites. Raman microscopy is used to study the purple areas of three species of diseased coral, Gorgonia ventalina, Pseudoplexaura porosa, and Eunicea laciniata obtained from Puerto Rico. These spectra were compared to Gorgonia ventalina samples previously reported that were obtained from San Salvador, Bahamas. Spectra from two samples of G. ventalina that had been infected by different agents, Aspergillus sydowii and a slime mold, were also obtained...
May 29, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/28587146/evaluation-of-the-dietary-intake-of-cadmium-lead-and-mercury-and-its-relationship-with-bone-health-among-postmenopausal-women-in-spain
#19
Luis M Puerto-Parejo, Ignacio Aliaga, María L Canal-Macias, Olga Leal-Hernandez, Raul Roncero-Martín, Sergio Rico-Martín, Jose M Moran
BACKGROUND: Heavy metals, such as lead, cadmium, and mercury, are absorbed through contaminated food sources and water. Few studies have investigated the extent to which dietary heavy metals are associated with low bone mineral density. AIMS: We aimed to characterize the dietary intake of the heavy metals lead, cadmium and mercury among healthy, non-smoking postmenopausal women in Spain. Furthermore, we sought to establish a putative relationship between bone health and the intake of these heavy metals in this population...
May 26, 2017: International Journal of Environmental Research and Public Health
https://www.readbyqxmd.com/read/28583702/barriers-to-genetic-testing-for-pediatric-medicaid-beneficiaries-with-epilepsy
#20
Eric J Kutscher, Sucheta M Joshi, Anup D Patel, Baria Hafeez, Zachary M Grinspan
BACKGROUND: Children with public insurance (Medicaid) have increased barriers to specialty care in the United States. For children with epilepsy, the relationship between public insurance and barriers to genetic testing is understudied. METHODS: We surveyed a sample of US child neurology clinicians. We performed quantitative and qualitative analysis of responses. RESULTS: There were 302 responses (of 1982 surveyed; response rate 15%) from clinicians from 46 states, the District of Columbia, and Puerto Rico, including board-certified child neurologists (82%), resident physicians (6%), nurses (3%), and nurse practitioners (3%)...
April 20, 2017: Pediatric Neurology
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