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Advanced glycation endproducts

Christoph Tondera, Markus Laube, Jens Pietzsch
The EF-hand type calcium-binding protein S100A12 exerts numerous intra- and extracellular functions of (patho)physiological relevance. Therefore, receptors of S100A12 are of high interest for research and clinical applications. Beside the extensively studied receptor for advanced glycation endproducts (RAGE), G-protein coupled receptors and more recently, scavenger receptors are suggested to be putative S100A12 receptors. Own findings and further information from the literature predestined CD36, a class B scavenger receptor, as promising candidate...
October 12, 2016: Amino Acids
Aleem Syed, Qiaochu Zhu, Emily A Smith
The effect of ligand on the lateral diffusion of receptor for advanced glycation endproducts (RAGE), a receptor involved in numerous pathological conditions, remains unknown. Single particle tracking experiments that use quantum dots specifically bound to hemagglutinin (HA)-tagged RAGE (HA-RAGE) are reported to elucidate the effect of ligand binding on HA-RAGE diffusion in GM07373 cell membranes. The ligand used in these studies is methylglyoxal modified-bovine serum albumin (MGO-BSA) containing advanced glycation end products modifications...
October 7, 2016: Biochimica et Biophysica Acta
Ewa Grzebyk, Agnieszka Piwowar
BACKGROUND: Advanced glycation endproducts (AGE) and advanced oxidation protein products (AOPP) arise as a result of excessive glycation and oxidation processes of proteins in hyperglycemia and oxidative stress conditions respectively, both in vivo and in vitro. In vivo these processes are especially intensified in patients with diabetes, and the adverse effects of AGE and AOPP are particularly unfavorable for the pathogenesis and aggravate the biochemical disturbances and clinical complications of diabetes...
September 29, 2016: BMC Complementary and Alternative Medicine
Mercedes Rodriguez-Teja, Claudia Breit, Mitchell Clarke, Kamil Talar, Kai Wang, Mohammad A Mohammad, Sage Pickwell, Guillermina Etchandy, Graeme J Stasiuk, Justin Sturge
Here we describe a protocol that can be used to study the biophysical microenvironment related to increased thickness and stiffness of the basement membrane (BM) during age-related pathologies and metabolic disorders (e.g. cancer, diabetes, microvascular disease, retinopathy, nephropathy and neuropathy). The premise of the model is non-enzymatic crosslinking of reconstituted BM (rBM) matrix by treatment with glycolaldehyde (GLA) to promote advanced glycation endproduct (AGE) generation via the Maillard reaction...
2016: Journal of Visualized Experiments: JoVE
Harveen Kaur, Meder Kamalov, Margaret A Brimble
In nature, proteins, lipids, and nucleic acids can nonenzymatically react with sugars and sugar degradation products to give rise to a diverse range of modifications, known as advanced glycation endproducts (AGEs). These AGEs typically occur at lysine and arginine residues of long-lived proteins, such as collagen, and can modify the structure and function of the native protein. AGEs accumulate during the normal aging process, and AGE formation is dramatically accelerated with diabetes. AGEs have also been implicated in a wide range of debilitating conditions including cardiovascular, renal failure, and neurodegenerative diseases...
October 18, 2016: Accounts of Chemical Research
Marta Navarro, Francisco J Morales
The antiglycative activity of hydroxytyrosol (HT) and olive leaf extract (OLE) was investigated in wheat-flour biscuits. Quercetin (QE) and gallic acid (GA) were used as reference of antiglycative activity of phenolic compounds. HT, OLE, QE and GA were added in the range of 0.25-0.75% (w/w). Samples were compared against a control recipe baked at 180°C/20min. HT biscuit was able to inhibit efficiently the formation of hydroxymethylfurfural (HMF) and 3-deoxyglucosone (3-DG), as well as reduced the formation of overall free fluorescent AGEs and pentosidine...
February 15, 2017: Food Chemistry
Jun Xie, Ran Li, Han Wu, Jianzhou Chen, Guannan Li, Qinhua Chen, Zhonghai Wei, Guixin He, Lian Wang, Albert Ferro, Biao Xu
Endothelial progenitor cells (EPCs) are a subtype of bone marrow-derived progenitor cells. Stromal cell-derived factor 1 (SDF-1)-mediated EPC mobilization from bone marrow to areas of ischemia plays an important role in angiogenesis. Previous studies have reported that advanced glycation endproducts (AGEs), which are important mediators of diabetes-related vascular pathology, may impair EPC migration and homing, but the mechanism is unclear. Syndecan-4 (synd4) is a ubiquitous heparan sulfate proteoglycan receptor on the cell surface, involved in SDF-1-dependent cell migration...
September 24, 2016: Stem Cells
Nicola Napoli, Manju Chandran, Dominique D Pierroz, Bo Abrahamsen, Ann V Schwartz, Serge L Ferrari
The risk of fragility fractures is increased in patients with either type 1 diabetes mellitus (T1DM) or type 2 diabetes mellitus (T2DM). Although BMD is decreased in T1DM, BMD in T2DM is often normal or even slightly elevated compared with an age-matched control population. However, in both T1DM and T2DM, bone turnover is decreased and the bone material properties and microstructure of bone are altered; the latter particularly so when microvascular complications are present. The pathophysiological mechanisms underlying bone fragility in diabetes mellitus are complex, and include hyperglycaemia, oxidative stress and the accumulation of advanced glycation endproducts that compromise collagen properties, increase marrow adiposity, release inflammatory factors and adipokines from visceral fat, and potentially alter the function of osteocytes...
September 23, 2016: Nature Reviews. Endocrinology
Emily R Koch, Permal Deo
BACKGROUND: Chronic hyperglycemia enhances the formation of advanced glycation endproducts (AGEs) and reactive oxygen species (ROS), contributing to diabetic complications. Thus, controlling blood glucose levels, inhibiting the formation of AGEs and reducing ROS are key therapeutic targets in early stage type 2 diabetes. METHODS: The inhibitory effects of seven commercial liquid nutritional supplements against carbohydrate hydrolysing enzymes, α-amylase and α-glucosidase, was determined by dinitrosalicylic (DNS) reagent and p-nitrophenyl-α-D-glucopyranoside solution, respectively...
2016: BMC Complementary and Alternative Medicine
Amrit Raj Ghosh, Roopkatha Bhattacharya, Shamik Bhattacharya, Titli Nargis, Oindrila Rahaman, Pritam Duttagupta, Deblina Raychaudhuri, Chinky Shiu Chen Liu, Shounak Roy, Parasar Ghosh, Shashi Khanna, Tamonas Chaudhuri, Om Tantia, Stefan Haak, Santu Bandyopadhyay, Satinath Mukhopadhyay, Partha Chakrabarti, Dipyaman Ganguly
In obese individuals the visceral adipose tissue (VAT) becomes seat of chronic low grade inflammation (metaflammation). But the mechanistic link between increased adiposity and metaflammation remains largely unclear. We report here that in obese individuals deregulation of a specific adipokine, chemerin, contributes to innate initiation of metaflammation, by recruiting circulating plasmacytoid dendritic cells (pDCs) into visceral adipose tissue via chemokine-like receptor 1 (CMKLR1). Adipose tissue-derived high mobility group B1 (HMGB1) protein, activates toll-like receptor 9 (TLR9) in the adipose-recruited pDCs by transporting extracellular DNA via receptor for advanced glycation endproducts (RAGE) and induces production of type I interferons...
August 25, 2016: Diabetes
John H Meertens, Hans L Nienhuis, Joop D Lefrandt, Casper G Schalkwijk, Kristiina Nyyssönen, Jack J M Ligtenberg, Andries J Smit, Jan G Zijlstra, D J Mulder
BACKGROUND: Advanced glycation end products (AGEs) have been implicated in multiple organ failure, predominantly via their cellular receptor (RAGE) in preclinical studies. Little is known about the time course and prognostic relevance of AGEs in critically ill human patients, including those with severe sepsis. OBJECTIVE: 1) To explore the reliability of Skin Autofluorescence (AF) as an index of tissue AGEs in ICU patients, 2) to compare its levels to healthy controls, 3) to describe the time course of AGEs and influencing factors during ICU admission, and 4) to explore their association with disease severity, outcome, and markers of oxidative stress and inflammation...
2016: PloS One
Ali Aljohi, Sabine Matou-Nasri, Nessar Ahmed
The accumulation of advanced glycation endproducts (AGEs) and oxidative stress underlie the pathogenesis of diabetic complications. In many developing countries, diabetes treatment is unaffordable, and plants such as bitter gourd (or bitter melon; Momordica charantia) are used as traditional remedies because they exhibit hypoglycaemic properties. This study compared the antiglycation and antioxidant properties of aqueous extracts of M. charantia pulp (MCP), flesh (MCF) and charantin in vitro. Lysozyme was mixed with methylglyoxal and 0-15 mg/ml of M...
2016: PloS One
Christiane Rüster, Sybille Franke, Stefanie Reuter, Ralf Mrowka, Tzvetanka Bondeva, Gunter Wolf
BACKGROUND/AIMS: We have previously shown that advanced glycation-endproducts (AGEs) induced NFκB activation in differentiated mouse podocytes. This NFκB activation may contribute to the progression of renal disease and mediation of fibrosis by various mechanisms. This study was undertaken to test whether this detrimental response may be reversed by vitamin D3 or its analogue paricalcitol. METHODS: Differentiated mouse podocytes were challenged with glycated bovine serum albumin (AGE-BSA), or non-glycated control BSA (in the presence or absence of various concentrations of vitamin D3 (decostriol, 1α,25-dihydroxyvitamin D3)) or its active analog paricalcitol...
2016: Nephron
Xiaodi Tian, Zhong Wang, Gang Chen
High mobility group box-1 (HMGB1), a highly conserved nonhistone nuclear protein, is widely expressed in most eukaryotic cells including neural cells. Nuclear HMGB1 stabilize nucleosome formation and facilitates gene transcription. HMGB1 can be passively released from necrotic cells or actively secreted from stimulated immune cells. Extracellular HMGB1 interacts with receptors, including the receptor for advanced glycation endproducts (RAGEs), Toll-like receptor 2 (TLR2) and TLR4. After brain injury, HMGB1 is released early from neural cells and contribute to the early stages of the inflammatory response...
August 8, 2016: Current Drug Delivery
Julien Pottecher, Anne-Claude Roche, Tristan Dégot, Olivier Helms, Jean-Gustave Hentz, Jean-Paul Schmitt, Pierre-Emmanuel Falcoz, Nicola Santelmo, François Levy, Olivier Collange, Béatrice Uring-Lambert, Siamak Bahram, Mickaël Schaeffer, Nicolas Meyer, Bernard Geny, Philippe Lassalle, Pierre Diemunsch, Gilbert Massard, Romain Kessler, Annick Steib
BACKGROUND: After lung transplantation (LT), early prediction of grade 3 pulmonary graft dysfunction (PGD) remains a research gap for clinicians. We hypothesized that it could be improved using extravascular lung water (EVLWi) and plasma biomarkers of acute lung injury. METHODS: After institutional review board approval and informed consent, consecutive LT recipients were included. Transpulmonary thermodilution-based EVLWi, plasma concentrations of epithelial (soluble receptor for advanced glycation endproducts [sRAGE]) and endothelial biomarkers (soluble intercellular adhesion molecule-1 [sICAM-1] and endocan [full-length and cleaved p14 fragment]) were obtained before and after LT (0 [H0], 6, 12, 24, 48 and 72h after pulmonary artery unclamping)...
August 5, 2016: Transplantation
Monika Pruenster, Thomas Vogl, Johannes Roth, Markus Sperandio
Neutrophils and monocytes belong to the first line of immune defence cells and are recruited to sites of inflammation during infection or sterile injury. Both cells contain huge amounts of the heterodimeric protein S100A8/A9 in their cytoplasm. S100A8/A9 belongs to the Ca(2+) binding S100 protein family and has recently gained a lot of interest as a critical alarm in modulating the inflammatory response after its release (extracellular S100A8/A9) from neutrophils and monocytes. Extracellular S100A8/A9 interacts with the pattern recognition receptors Toll-like receptor 4 (TLR4) and Receptor for Advanced Glycation Endproducts (RAGE) promoting cell activation and recruitment...
August 1, 2016: Pharmacology & Therapeutics
Takeshi Nishida, Maho Tsubota, Yudai Kawaishi, Hiroki Yamanishi, Natsuki Kamitani, Fumiko Sekiguchi, Hiroyasu Ishikura, Keyue Liu, Masahiro Nishibori, Atsufumi Kawabata
Given that high mobility group box 1 (HMGB1), a nuclear protein, once released to the extracellular space, promotes nociception, we asked if inactivation of HMGB1 prevents or reverses chemotherapy-induced painful neuropathy in rats and also examined possible involvement of Toll-like receptor 4 (TLR4) and the receptor for advanced glycation endproduct (RAGE), known as targets for HMGB1. Painful neuropathy was produced by repeated i.p. administration of paclitaxel or vincristine in rats. Nociceptive threshold was determined by the paw pressure method and/or von Frey test in the hindpaw...
July 15, 2016: Toxicology
Theodoros Eleftheriadis, Konstantina Tsogka, Georgios Pissas, Georgia Antoniadi, Vassilios Liakopoulos, Ioannis Stefanidis
PURPOSE: Considering the referred beneficial effects of protein restriction on diabetic nephropathy (DN) and the role of renal endothelium in its pathogenesis, we evaluated the effect of general control nonderepressible 2 (GCN2) kinase activation, a sensor of amino acid deprivation, on known detrimental molecular pathways in primary human glomerular endothelial cells (GEnC). METHODS: GEnC were cultured under normal or high-glucose conditions in the presence or not of the GCN2 kinase activator, tryptophanol...
October 2016: International Urology and Nephrology
Ann V Schwartz
Diabetes is characterized by increased fracture risk and by reduced bone strength for a given density. Contributing factors may include lower bone turnover and accumulation of advanced glycation endproducts. There are concerns that the pharmacological therapies for osteoporosis, particularly anti-resorptive therapies that suppress bone turnover, may not be as effective in the setting of diabetes. This review considers clinical trials and observational studies that have assessed the efficacy of anti-resorptive and anabolic therapies in diabetic patients...
July 26, 2016: Calcified Tissue International
Zsuzsanna Géhl, Edina Bakondi, Miklós D Resch, Csaba Hegedűs, Katalin Kovács, Petra Lakatos, Antal Szabó, Zoltán Nagy, László Virág
PURPOSE: Diabetes is accompanied by fundamental rearrangements in redox homeostasis. Hyperglycemia triggers the production of reactive oxygen and nitrogen species which contributes to tissue damage in various target organs. Proliferative diabetic retinopathy (PDR) is a common manifestation of diabetic complications but information on the possible role of reactive intermediates in this condition with special regard to the involvement of the vitreous in PDR-associated redox alterations is scarce...
July 8, 2016: Redox Biology
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