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Jessica A Zinskie, Michael Shribak, Michael F Bruist, Karl J Aufderheide, Chris Janetopoulos
In order to obtain fine details in 3 dimensions (3D) over time, it is critical for motile biological specimens to be appropriately immobilized. Of the many immobilization options available, the mechanical microcompressor offers many benefits. Our device, previously described, achieves gentle flattening of a cell, allowing us to image finely detailed structures of numerous organelles and physiological processes in living cells. We have imaged protozoa and other small metazoans using differential interference contrast (DIC) microscopy, orientation-independent (OI) DIC, and real-time birefringence imaging using a video-enhanced polychromatic polscope...
October 1, 2015: Experimental Cell Research
Yingjun Yan, Liwei Jiang, Karl J Aufderheide, Gus A Wright, Alexander Terekhov, Lino Costa, Kevin Qin, W Tyler McCleery, John J Fellenstein, Alessandro Ustione, J Brian Robertson, Carl Hirschie Johnson, David W Piston, M Shane Hutson, John P Wikswo, William Hofmeister, Chris Janetopoulos
A microcompressor is a precision mechanical device that flattens and immobilizes living cells and small organisms for optical microscopy, allowing enhanced visualization of sub-cellular structures and organelles. We have developed an easily fabricated device, which can be equipped with microfluidics, permitting the addition of media or chemicals during observation. This device can be used on both upright and inverted microscopes. The apparatus permits micrometer precision flattening for nondestructive immobilization of specimens as small as a bacterium, while also accommodating larger specimens, such as Caenorhabditis elegans, for long-term observations...
February 2014: Microscopy and Microanalysis
Karl J Aufderheide
Microscopists making observations on living cells are often faced with the challenge of getting those cells to hold still for extended observation times. This paper presents an overview/summary of a range of techniques for non-destructive immobilization of living cells (with an emphasis on protozoa), permitting microscopic observations and photography. A variety of chemical and physical immobilization techniques are discussed, but particular attention is paid to a comparative discussion of the mechanical devices (rotocompressors or microcompressors) used for reversible trapping of living cells...
2008: Micron: the International Research and Review Journal for Microscopy
O A Kamyshanskiĭ, E I Sham
No abstract text is available yet for this article.
1980: Laboratornoe Delo
L S Samben
No abstract text is available yet for this article.
1967: Novosti Meditsinskogo Priborostroeniia
S B Daniiarov, Iu M Lanskiĭ, E M Bebinov
A design of dry spirograph is described. It is characterized by greater precision, lack of inertia, high reliability, absence of respiration resistance, adequate form of recording, rapid resetting to any respiratory rate. The device consists of two similar injection syringes, photoelectric sensor for the identification of the initial moments of respiration stages, electromagnetic valves, two photoelectric converters of the air volume into the impulse signal, vacuum micro-pump, microcompressor and a system of air-driving tubes...
October 1986: Biulleten' Eksperimental'noĭ Biologii i Meditsiny
V S Borodianskiĭ, A E Shoĭkhet, A S Pinchuk, A N Ostapenko, S E Bonda'r
The results of treatment of 137 patients with post-injection abscesses are analysed. The specific weight of such abscesses in the general structure of purulent diseases was 10.8%. Diabetes mellitus was present in 19.7% of the patients. Seventy nine patients were treated by a conventional method. In 58 patients, the surgical treatment with the use of a drainage-irrigative system was employed. Of them, in 15 patients, the prolonged aspiration by means of the adapted VK-1 microcompressor were performed. In therapy of post-injection abscesses by a conventional method, the mean period of temporary invalidity was 37...
1989: Klinicheskaia Khirurgiia
D M Spoon
No abstract text is available yet for this article.
July 1978: Transactions of the American Microscopical Society
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