Read by QxMD icon Read

Non-tuberculous mycobacteria

Cecilia Brambilla, Marta Llorens-Fons, Esther Julián, Estela Noguera-Ortega, Cristina Tomàs-Martínez, Miriam Pérez-Trujillo, Thomas F Byrd, Fernando Alcaide, Marina Luquin
The rough morphotypes of non-tuberculous mycobacteria have been associated with the most severe illnesses in humans. This idea is consistent with the fact that Mycobacterium tuberculosis presents a stable rough morphotype. Unlike smooth morphotypes, the bacilli of rough morphotypes grow close together, leaving no spaces among them and forming large aggregates (clumps). Currently, the initial interaction of macrophages with clumps remains unclear. Thus, we infected J774 macrophages with bacterial suspensions of rough morphotypes of M...
2016: Frontiers in Microbiology
D Kušar, U Zajc, V Jenčič, M Ocepek, J Higgins, M Žolnir-Dovč, M Pate
Fish are commonly infected with non-tuberculous mycobacteria (NTM), which should be regarded as potential pathogens when handling aquarium fish and equipment. This study examined 107 aquarium fish from pet shops. Cultivation of the fish samples using different selective media was conducted for identification of NTM. Isolates were identified using the GenoType Mycobacterium common mycobacteria and additional species assays, sequencing of the 16S rRNA and rpoB genes, and real-time PCR assay for identification of Mycobacterium (M...
October 17, 2016: Journal of Fish Diseases
John Moat, Athanasios Rizoulis, Graeme Fox, Mathew Upton
The domestic environment can be a source of pathogenic bacteria. We show here that domestic shower hoses may harbour potentially pathogenic bacteria and fungi. Well-developed biofilms were physically removed from the internal surface of shower hoses collected in four locations in England and Scotland. Amplicon pyrosequencing of 16S and 18S rRNA targets revealed the presence of common aquatic and environmental bacteria, including members of the Actinobacteria, Alphaproteobacteria, Bacteroidetes and non-tuberculous Mycobacteria...
October 2016: Journal of Water and Health
Sabine Hofmann-Thiel, Nikolay Molodtsov, Uladzimir Antonenka, Harald Hoffmann
Abbott RealTime MTB (RT MTB) is a new automated nucleic acid amplification test for the detection of Mycobacterium tuberculosis complex (MTBC) in clinical specimens. In combination with RealTime MTB INH/RIF Resistance (RT MTB INH/RIF), which can be applied to RT MTB positive specimens as add-on assay, the tests also indicate genetic markers of resistance to isoniazid and rifampicin. We aimed to evaluate the diagnostic sensitivity and specificity of RT MTB using different types of respiratory and extra-pulmonary specimens and to compare performance characteristics directly with the FluoroType MTB assay...
October 12, 2016: Journal of Clinical Microbiology
R Kumar, R Garg, S Kant, S K Verma, R A S Kushwaha, S Kumar, A Jain
OBJECTIVE: To study the drug resistance profile of patients with suspected multidrug-resistant tuberculosis (MDR-TB). MATERIAL AND METHODS: This was a prospective study conducted among patients with suspected MDR-TB attending the Department of Respiratory Medicine, King George's Medical University, Lucknow, India, from August 2014 to April 2015. Sputum samples obtained from 50 such patients were subjected to drug susceptibility testing against first- and second-line drugs...
October 2016: International Journal of Tuberculosis and Lung Disease
Xia Yu, Pengnian Liu, Guan Liu, Liping Zhao, Yanjie Hu, Guomei Wei, Jingjing Luo, Hairong Huang
Despite increasing attention towards the non-tuberculous mycobacterial (NTM) diseases, the overall epidemiological information remains unavailable for China. A systematic review and meta-analysis was conducted using data of 105 qualified publications from Chinese mainland. The assay demonstrated that the prevalence of NTM infections among tuberculosis suspects was 6.3% (5.4%-7.4%) in mainland, while the Southeastern region had the highest NTM prevalence at 8.6% (7.1%-10.5%). In Northern China, slow growing mycobacteria (SGM) consistituted 63...
October 4, 2016: Journal of Infection
Ranu Surolia, Suman Karki, Zheng Wang, Tejaswini Kulkarni, Fu Jun Li, Shikhar Vohra, Hitesh Batra, Jerry A Nick, Steven R Duncan, Victor J Thannickal, Adrie Jc Steyn, Anupam Agarwal, Veena B Antony
Pulmonary infections with non-tuberculous mycobacteria (P-NTM) such as by Mycobacterium avium complex (M. avium) are increasingly found in the elderly, but the underlying mechanisms are unclear. Recent studies suggest that adaptive immunity is necessary but not sufficient for host defense against mycobacteria. Heme oxygenase-1 (HO-1) has been recognized as a critical modulator of granuloma formation and programmed cell death in mycobacterial infections. Old mice (18-21 months) infected with M. avium had attenuated HO-1 response with diffuse inflammation, high burden of mycobacteria, poor granuloma formation, and decreased survival (45%) while young mice (4-6 months) showed tight, well defined granuloma, increased HO-1 expression and increased survival (95%)...
September 30, 2016: American Journal of Physiology. Lung Cellular and Molecular Physiology
Seow Hoon Saw, Joon Liang Tan, Xin Yue Chan, Kok Gan Chan, Yun Fong Ngeow
BACKGROUND: Meningitis is a major cause of mortality in tuberculosis (TB). It is not clear what factors promote central nervous system invasion and pathology but it has been reported that certain strains of Mycobacterium tuberculosis (Mtb) might have genetic traits associated with neurotropism. METHODS: In this study, we generated whole genome sequences of eight clinical strains of Mtb that were isolated from the cerebrospinal fluid (CSF) of patients presenting with tuberculous meningitis (TBM) in Malaysia, and compared them to the genomes of H37Rv and other respiratory Mtb genomes either downloaded from public databases or extracted from local sputum isolates...
2016: PeerJ
Xuzhou Wang, Feilai Xie, Qiaoling Zheng, Xingfeng Qi, Min Li, Xiaoling Zhou, Zhiyong Zheng
AIMS: This study aimed to use EDTA to retrieve paraffin-embedded tissue sections of inflammatory granulomatous lesions and increase the detection rate of tuberculosis (TB)/non-tuberculous mycobacteria. Due to the influence of chemical reagents during the fixation process, the amplification of fluorescent quantitative PCR was blocked after DNA extraction, and the results were not ideal. METHODS: Special staining technologies (acid-fast and Auramine O) and fluorescent quantitative PCR were used to detect TB/non-tuberculous mycobacteria in 125 cases of inflammatory granulomatous lesions in paraffin-embedded tissue sections with and without EDTA retrieval...
September 29, 2016: Journal of Clinical Pathology
Guma Abdeldaim, Erik Svensson, Jonas Blomberg, Björn Herrmann
A duplex real-time PCR based on the rnpB gene was developed for Mycobacterium spp. The assay was specific for the Mycobacterium tuberculosis complex (MTB) and also detected all 19 tested species of non-tuberculous mycobacteria (NTM). The assay was evaluated on 404 clinical samples: 290 respiratory samples and 114 from tissue and other non-respiratory body sites. M. tuberculosis was detected by culture in 40 samples and in 30 samples by the assay. The MTB assay showed a sensitivity similar to Roche Cobas Amplicor MTB-PCR (Roche Molecular Systems, Pleasanton, CA, USA)...
November 2016: APMIS: Acta Pathologica, Microbiologica, et Immunologica Scandinavica
Oren Mayer, Paras Jain, Torin Weisbrod, Daniel Biro, Libby Ho, Deborah Jacobs-Sera, Graham F Hatfull, William R Jacobs
: Mycobacteriophage DS6A is unique amongst the more than 8000 isolated mycobacteriophages due to its ability to form plaques exclusively on mycobacteria belonging to the Mycobacterium tuberculosis Complex (MTBC). Speculation surrounding this specificity has led to unsupported assertions in published studies and patents that non-tuberculous mycobacteria (NTM) are wholly resistant to DS6A infection. In this study, we identified two independent, nonessential regions in the DS6A genome and replaced them with an mVenus-expressing plasmid to generate fluorescent reporter phages Φ(2)GFP12 and Φ(2)GFP13...
September 26, 2016: Journal of Bacteriology
Manoj Kumar, Sheetal Verma, Tapan N Dhole, Raj Kumar
A 34 year-old woman presented with history of progressive back pain which was diagnosed as Pott's disease with recurrent paravertebral abscess, and had received a total of 23 months of antitubercular treatment for lumbar infection. The patient was non-responsive to treatment and was referred to a higher centre where the infection was finally identified as non-tuberculous mycobacterium (Mycobacterium fortuitum) by genotypic testing. The drugs were initiated according to results of drug susceptibility testing by the broth microdilution method...
2016: BMJ Case Reports
Hyunjung Kim, Jehoon Lee, Hi Jeong Kwon, Kwan Hyoung Kim, Chang Dong Yeo, Jin Woo Kim, Hae Kyung Lee
BACKGROUND: This study compared the diagnostic power of isothermal target and probe amplification (iTPA) with the existing real-time PCR for the detection of Mycobacterium tuberculosis (MTB). METHOD: The two molecular methods were performed using DNA extracted directly from lower respiratory tract samples, not from the culture broth. A total of 174 non-consecutive patients with suspected pulmonary tuberculosis were enrolled in this study. Acid-fast bacilli (AFB) stain and liquid culture with the BACTEC MGIT 960 system (Becton Dickinson Diagnostic, USA) were performed...
September 2016: Annals of Clinical and Laboratory Science
Feifei Yin, Jasper Fuk-Woo Chan, Qixuan Zhu, Ruijia Fu, Jonathan Hon-Kwan Chen, Garnet Kwan-Yue Choi, Kah-Meng Tee, Lihua Li, Shiuyun Qian, Wing-Cheong Yam, Gang Lu, Kwok-Yung Yuen
AIMS: Rapid and accurate diagnostic assays with simultaneous microbial identification and drug resistance detection are essential for optimising treatment and control of tuberculosis. METHODS: We developed a novel multiplex (TRIOL, Tuberculosis-Rifampicin-Isoniazid-Ofloxacin-Luminex) assay using the Luminex xMAP system that simultaneously identifies Mycobacterium tuberculosis and detects resistance to first-line and second-line anti-tuberculous drugs, and compared its performance with that by PCR sequencing, using phenotypic drug susceptibility testing as the gold standard...
September 19, 2016: Journal of Clinical Pathology
I Porvaznik, I Solovič, J Mokrý
Non-tuberculous mycobacteria (NTM) are species other than those belonging to the Mycobacterium tuberculosis complex and do not cause leprosy. NTM are generally free-living organisms that are ubiquitous in the environment. There have been more than 140 NTM species identified to-date. They can cause a wide range of infections, with pulmonary infections being the most frequent (65-90 %). There is growing evidence that the incidence of NTM lung diseases and associated hospitalizations are on the rise, mainly in regions with a low prevalence of tuberculosis...
September 20, 2016: Advances in Experimental Medicine and Biology
D Huet, B Godbert, J Hermann, J-M Zordan, F Chabot, C Andréjak
INTRODUCTION: Pulmonary infection due to Mycobacterium malmoense can be difficult to diagnose. These difficulties can be responsible for a delay in the implementation of optimal treatment. Moreover, the treatment is not standardized. OBSERVATION: We report the case of a 56-year-old patient who developed a Mycobacterium malmoense pulmonary infection whose diagnosis was delayed due to initial suspicion of pulmonary Mycobacterium tuberculosis infection. Once the diagnosis was confirmed, the patient was treated empirically with rifampicin, ethambutol, and clarithromycin for 12 months after culture conversion, giving a total of 15 months...
September 14, 2016: Revue des Maladies Respiratoires
Haekyung Lee, Dohui Hwang, Minchul Jeon, Eunjung Lee, Taehyong Kim, Shi Nae Yu, Yongbeom Kim, Byung-Ill Lee
BACKGROUND: There are increasing reports on nosocomial Mycobacterium massiliense infection, but septic arthritis and osteomyelitis because of that microorganism is rare. This report focuses on the clinical aspects of M. massiliense arthritis outbreak concurrent with soft tissue infection. CASE PRESENTATION: An outbreak of septic arthritis among patients who had been injected at a single clinic occurred in South Korea between April and September 2012. This may be associated with repeated injection of triamcinolone contaminated with M...
2016: BMC Research Notes
Nurul Yaqeen Mohd Esa, Adli Azam Mohd Radzi, Nor Salmah Bakar, Mohd Shukry Mohd Khalid, Ahmad Izuanuddin Ismail, Mohamed Fauzi Abdul Rani
Teratomas of anterior mediastinum are rare. They are often slow growing, asymptomatic, and detected incidentally on chest imaging. Mycobacterium abscessus (M. abscessus) is an acid-fast bacillus that is classified as a pathogenic "rapid growing" non-tuberculous mycobacteria. It is an uncommon cause of human pathology, which may cause skin and soft tissue infection after skin injury following inoculation, minor trauma, and surgery. Here, we present an unusual case of benign cystic teratoma mimicking recurrent pleural effusion, which was subsequently complicated by M...
May 2016: Respirology Case Reports
J Monteserin, R Paul, B Lopez, M Cnockaert, E Tortoli, C Menéndez, M J García, J C Palomino, P Vandamme, V Ritacco, A Martin
SETTING: Over 150 potentially pathogenic non-tuberculous mycobacteria (NTM) species have been described, posing an onerous challenge for clinical laboratory diagnosis. OBJECTIVE: To evaluate different approaches for the identification of 40 clinically relevant NTM isolates whose species were not reliably identified using our routine diagnostic workflow comprising phenotypic tests and hsp65 polymerase chain reaction restriction analysis. DESIGN: We used 1) sequencing analysis of four conserved gene targets: 16S rRNA, rpoB, hsp65 and sodA; 2) two commercial reverse hybridisation assays; and 3) protein analysis using matrix-assisted laser desorption/ionisation time of flight mass spectrometry (MALDI-TOF MS)...
September 2016: International Journal of Tuberculosis and Lung Disease
H S Schaaf, A J Garcia-Prats, K du Preez, C Rautenbach, A C Hesseling
BACKGROUND: Longer-term tuberculosis (TB) drug resistance surveillance among children is rare. We determined the prevalence of drug resistance among children with culture-confirmed TB from 2011 to 2013, compared these results with four previous consecutive 2-year periods and documented other mycobacterial isolates identified. METHOD: Surveillance study of mycobacterial culture in all children aged <13 years conducted from March 2011 to February 2013 at the Tygerberg Children's Hospital, Cape Town, South Africa...
September 2016: International Journal of Tuberculosis and Lung Disease
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"