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https://www.readbyqxmd.com/read/29783174/facile-isolation-of-%C3%AE-ribazole-from-vitamin-b-12-hydrolysates-using-boronate-affinity-chromatography
#1
Theodoric A Mattes, Jorge C Escalante-Semerena
Alpha-ribazole (α-R) is a unique riboside found in the nucleotide loop of coenzyme B12 (CoB12 ). α-R is not an intermediate of the de novo biosynthetic pathway of coenzyme B12 , but some bacteria of the phylum Firmicutes have evolved a two-protein system (transporter, kinase) that scavenges α-R from the environment and converts it to the pathway intermediate α-RP. Since α-R is not commercially available, one must either synthesize α-R, or isolate it from hydrolysates of vitamin B12 (cyano-B12 , CNB12 ), so the function of the above-mentioned proteins can be studied...
May 16, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29782945/advances-in-the-slow-freezing-cryopreservation-of-microencapsulated-cells
#2
REVIEW
H Gurruchaga, L Saenz Del Burgo, R M Hernandez, G Orive, C Selden, B Fuller, J Ciriza, J L Pedraz
Over the past few decades, the use of cell microencapsulation technology has been promoted for a wide range of applications as sustained drug delivery systems or as cells containing biosystems for regenerative medicine. However, difficulty in their preservation and storage has limited their availability to healthcare centers. Because the preservation in cryogenic temperatures poses many biological and biophysical challenges and that the technology has not been well understood, the slow cooling cryopreservation, which is the most used technique worldwide, has not given full measure of its full potential application yet...
May 18, 2018: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/29782801/epr-evidence-of-liquid-water-in-ice-an-intrinsic-property-of-water-or-a-self-confinement-effect
#3
Muthulakshmi Thangswamy, Priya Maheshwari, Dhanadeep Dutta, Vinayak G Rane, Pradeep Kumar Pujari
Liquid water (LW) existence in pure ice below 273 K has been a controversial aspect primarily because of the lack of experimental evidences. Recently, electron paramagnetic resonance (EPR) has been used to study deeply supercooled water in a rapidly-frozen polycrystalline ice. The same technique can also be used to probe the presence of LW in polycrystalline ice that has formed through a more conventional, slow cooling one. In this context, the present study aims to emphasize that in case of an external probe involving techniques such as EPR, the results are influenced by the binary phase (BP) diagram of the probe-water system, which also predicts the existence of LW domains in ice, upto the eutectic point...
May 21, 2018: Journal of Physical Chemistry. A
https://www.readbyqxmd.com/read/29782183/effect-of-cabbage-wraps-on-the-reduction-of-post-traumatic-knee-exudates-in-men
#4
Jacek Dygut, Monika Piwowar, Kinga Fijałkowska, Ibeth Guevara, Robert Jakubas, Gustaw Gonzales, Krzysztof Popławski, Anna Strokowska, Hanna Wikariak, Wiktor Jurkowski
OBJECTIVES: The study investigates measurable effects of cabbage leaf wraps on post-traumatic knee injury exudate absorption in men. DESIGN: Case-control experiment on the same group of patients (before and after treatment). SETTINGS/LOCATION: One academic center and two hospitals. SUBJECTS: The study was carried out on a group of patients with different degrees of injury severity in the acute stage of the knee injury who were divided into three groups based on the width of suprapatellar recess gap (3-5 mm in group 1, 6-10 mm in group 2, and 11 mm or more in group 3) as assessed by ultrasonography...
May 21, 2018: Journal of Alternative and Complementary Medicine: Research on Paradigm, Practice, and Policy
https://www.readbyqxmd.com/read/29782158/fluorescigenic-magnetofluids-based-on-gadolinium-terbium-and-dysprosium-containing-imidazolium-salts
#5
Kun-Lun Cheng, Wen-Li Yuan, Ling He, Ning Tang, Hong-Mei Jian, Ying Zhao, Song Qin, Guo-Hong Tao
Multistimuli responsive soft materials are urgently needed in many different fields, such as anticounterfeiting technology and microdroplet manipulation. Herein, the straightforward preparation of fluorescigenic magnetofluids by the introduction of the paramagnetic metal ions Gd3+ , Tb3+ , and Dy3+ into alkylimidazolium-based ionic liquids (ILs) is reported. Bright visible fluorescence was observed under UV irradiation for Tb- and Dy-containing ILs. Either pure samples or papers coated with these ILs exhibited pronounced magnetic responses...
May 21, 2018: Inorganic Chemistry
https://www.readbyqxmd.com/read/29782015/experimental-procedure-for-laboratory-studies-of-in-situ-burning-flammability-and-burning-efficiency-of-crude-oil
#6
Laurens van Gelderen, Grunde Jomaas
A new method for the simultaneous study of the flammability and burning efficiency of fresh and weathered crude oil through two experimental laboratory setups is presented. The experiments are easily repeatable compared to operational scale experiments (pool diameter ≥2 m), while still featuring quite realistic in situ burning conditions of crude oil on water. Experimental conditions include a flowing water sub-layer that cools the oil slick and an external heat flux (up to 50 kW/m2 ) that simulates the higher heat feedback to the fuel surface in operational scale crude oil pool fires...
May 1, 2018: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29781805/hot-electron-cooling-in-dirac-semimetal-cd-sub-3-sub-as-sub-2-sub-due-to-polar-optical-phonons
#7
Shrishail S Kubakaddi, Tutul Biswas
A theory of hot electron cooling power due to polar optical phonons $P_{\rm op}$ is developed in
 three-dimensional Dirac semimetal($3$DDS) Cd$_3$As$_2$ taking account of hot phonon effect. Hot phonon
 distribution $N_q$ and $P_{\rm op}$ are investigated as a function of electron temperature $T_e$,
 electron density $n_e$, and phonon relaxation time $\tau_p$. It is found that $P_{\rm op}$ increases
 rapidly (slowly) with $T_e$ at lower (higher) temperature regime.
 Whereas, $P_{\rm op}$ is weakly decreasing with increasing $n_e$...
May 21, 2018: Journal of Physics. Condensed Matter: An Institute of Physics Journal
https://www.readbyqxmd.com/read/29781294/microbiota-in-a-cooling-lubrication-circuit-and-an-option-for-controlling-triethanolamine-biodegradation
#8
Thomas Klammsteiner, Heribert Insam, Maraike Probst
Cooling and lubrication agents like triethanolamine (TEA) are essential for many purposes in industry. Due to biodegradation, they need continuous replacement, and byproducts of degradation may be toxic. This study investigates an industrial (1,200 m³) cooling-lubrication circuit (CLC) that has been in operation for 20 years and is supposedly in an ecological equilibrium, thus offering a unique habitat. Next-generation (Illumina Miseq 16S rRNA amplicon) sequencing was used to profile the CLC-based microbiota and relate it to TEA and bicine dynamics at the sampling sites, influent, machine rooms, biofilms and effluent...
May 21, 2018: Biofouling
https://www.readbyqxmd.com/read/29781267/highly-regular-uniform-k-3-scf-6-mn-4-phosphors-facile-synthesis-microstructures-photo-luminescence-properties-and-application-in-led-devices
#9
Hong Ming, Shuifu Liu, Lili Liu, Jiaqing Peng, Junxiang Fu, Fu Du, Xinyu Ye
A new generation of red phosphors of complex fluoride matrices activated with Mn4+ , has gained a broad interest in getting high color rendering index (CRI), low correlated color temperature (CCT) of white light-emitting diodes(WLEDs). However, besides their instability towards moisture, the extremely irregular and nonuniform morphologies of these phosphors have limited their practical industry applications. In the present study, a novel type of K3 ScF6 :Mn4+ red phosphor with highly regular, uniform and high color purity was synthesized successfully via a facile two-step co-precipitation method under mild conditions...
May 21, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29781253/developmental-plasticity-in-reptiles-insights-from-temperature-dependent-gene-expression-in-wall-lizard-embryos
#10
Nathalie Feiner, Alfredo Rago, Geoffrey M While, Tobias Uller
Many features of the development of reptiles are affected by temperature, but very little is known about how incubation temperature affects gene expression. Here, we provide a detailed case study of gene expression profiles in common wall lizard (Podarcis muralis) embryos developing at stressfully low (15°C) versus benign (24°C) temperature. For maximum comparability between the two temperature regimes, we selected a precise developmental stage early in embryogenesis defined by the number of somites. We used a split-clutch design and lizards from four different populations to evaluate the robustness of temperature-responsive gene expression profiles...
May 20, 2018: Journal of experimental zoology. Part A, Ecological and integrative physiology
https://www.readbyqxmd.com/read/29780677/the-2016-southeastern-us-drought-an-extreme-departure-from-centennial-wetting-and-cooling
#11
A Park Williams, Benjamin I Cook, Jason E Smerdon, Daniel A Bishop, Richard Seager, Justin S Mankin
The fall 2016 drought in the southeastern United States (SE US) appeared exceptional based on its widespread impacts, but the current monitoring framework that only extends from 1979-present does not readily facilitate evaluation of soil-moisture anomalies in a centennial context. A new method to extend monthly gridded soil-moisture estimates back to 1895 is developed, indicating that since 1895, October-November 2016 soil moisture (0-200 cm) in the SE US was likely the second lowest on record, behind 1954...
October 27, 2017: Journal of Geophysical Research. Atmospheres: JGR
https://www.readbyqxmd.com/read/29780665/knowing-your-neighbourhood-the-effects-of-epichlo%C3%A3-endophytes-on-foliar-fungal-assemblages-in-perennial-ryegrass-in-dependence-of-season-and-land-use-intensity
#12
Julia König, Marco Alexandre Guerreiro, Derek Peršoh, Dominik Begerow, Jochen Krauss
Epichloë endophytes associated with cool-season grass species can protect their hosts from herbivory and can suppress mycorrhizal colonization of the hosts' roots. However, little is known about whether or not Epichloë endophyte infection can also change the foliar fungal assemblages of the host. We tested 52 grassland study sites along a land-use intensity gradient in three study regions over two seasons (spring vs. summer) to determine whether Epichloë infection of the host grass Lolium perenne changes the fungal community structure in leaves...
2018: PeerJ
https://www.readbyqxmd.com/read/29780552/experiments-indicating-a-second-hydrogen-ordered-phase-of-ice-vi
#13
Tobias M Gasser, Alexander V Thoeny, Lucie J Plaga, Karsten W Köster, Martin Etter, Roland Böhmer, Thomas Loerting
In the last twelve years five new ice phases were experimentally prepared. Two of them are empty clathrate hydrates and three of them represent hydrogen ordered counterparts of previously known disordered ice phases. Here, we report on hydrogen ordering in ice VI samples produced by cooling at pressures up to 2.00 GPa. Based on results from calorimetry, dielectric relaxation spectroscopy, Raman spectroscopy, and powder X-ray diffraction the existence of a second hydrogen ordered polymorph related to ice VI is suggested...
May 14, 2018: Chemical Science
https://www.readbyqxmd.com/read/29780544/the-enhancement-and-suppression-of-immersion-mode-heterogeneous-ice-nucleation-by-solutes
#14
Thomas F Whale, Mark A Holden, Theodore W Wilson, Daniel O'Sullivan, Benjamin J Murray
Heterogeneous nucleation of ice from aqueous solutions is an important yet poorly understood process in multiple fields, not least the atmospheric sciences where it impacts the formation and properties of clouds. In the atmosphere ice-nucleating particles are usually, if not always, mixed with soluble material. However, the impact of this soluble material on ice nucleation is poorly understood. In the atmospheric community the current paradigm for freezing under mixed phase cloud conditions is that dilute solutions will not influence heterogeneous freezing...
May 7, 2018: Chemical Science
https://www.readbyqxmd.com/read/29780536/thermoreversible-crystallization-driven-aggregation-of-diblock-copolymer-nanoparticles-in-mineral-oil
#15
Matthew J Derry, Oleksandr O Mykhaylyk, Anthony J Ryan, Steven P Armes
A poly(behenyl methacrylate)37 (PBeMA37 ) macromolecular chain transfer agent is utilized for the reversible addition-fragmentation chain transfer (RAFT) dispersion polymerization of benzyl methacrylate (BzMA) directly in mineral oil at 90 °C. Polymerization-induced self-assembly (PISA) occurs under these conditions, yielding a series of sterically-stabilized PBeMA37 -PBzMA x diblock copolymer spheres of tunable diameter as confirmed by dynamic light scattering (DLS) and transmission electron microscopy (TEM) studies...
May 7, 2018: Chemical Science
https://www.readbyqxmd.com/read/29780509/self-complementary-nickel-halides-enable-multifaceted-comparisons-of-intermolecular-halogen-bonds-fluoride-ligands-vs-other-halides
#16
Vargini Thangavadivale, Pedro M Aguiar, Naseralla A Jasim, Sarah J Pike, Dan A Smith, Adrian C Whitwood, Lee Brammer, Robin N Perutz
The syntheses of three series of complexes designed with self-complementary motifs for formation of halogen bonds between an iodotetrafluorophenyl ligand and a halide ligand at square-planar nickel are reported, allowing structural comparisons of halogen bonding between all four halides C6 F4 I···X-Ni (X = F, Cl, Br, I). In the series trans -[NiX(2,3,5,6-C6 F4 I)(PEt3 )2 ] 1pX and trans -[NiX(2,3,4,5-C6 F4 I)(PEt3 )2 ] (X = F, Cl, Br, I) 1oX , the iodine substituent on the benzene ring was positioned para and ortho to the metal, respectively...
April 21, 2018: Chemical Science
https://www.readbyqxmd.com/read/29780175/rapid-identification-of-a-cooling-tower-associated-legionnaires-disease-outbreak-supported-by-polymerase-chain-reaction-testing-of-environmental-samples-new-york-city-2014-2015
#17
Isaac Benowitz, Robert Fitzhenry, Christopher Boyd, Michelle Dickinson, Michael Levy, Ying Lin, Elizabeth Nazarian, Belinda Ostrowsky, Teresa Passaretti, Jennifer Rakeman, Amy Saylors, Elena Shamoonian, Terry-Ann Smith, Sharon Balter
We investigated an outbreak of eight Legionnaires' disease cases among persons living in an urban residential community of 60,000 people. Possible environmental sources included two active cooling towers (air-conditioning units for large buildings) <1 km from patient residences, a market misting system, a community-wide water system used for heating and cooling, and potable water. To support a timely public health response, we used real-time polymerase chain reaction (PCR) to identify Legionella DNA in environmental samples within hours of specimen collection...
April 2018: Journal of Environmental Health
https://www.readbyqxmd.com/read/29779996/cool-intentions-adding-refrigerators-to-elementary-classrooms-improves-fruit-and-vegetable-access-in-a-pilot-study
#18
Rebecca Boehm, Tara J Schuknecht, Sean B Cash
No abstract text is available yet for this article.
May 18, 2018: Journal of Nutrition Education and Behavior
https://www.readbyqxmd.com/read/29779063/development-of-sperm-vitrification-protocols-for-two-endangered-salmonid-species-the-adriatic-grayling-thymallus-thymallus-and-the-marble-trout-salmo-marmoratus
#19
Eszter Kása, Jelena Lujić, Zoran Marinović, Tímea Kollár, Gergely Bernáth, Zoltán Bokor, Béla Urbányi, Kinga Katalin Lefler, Dušan Jesenšek, Ákos Horváth
Vitrification was applied to the sperm of two endangered fish species of Soča River basin in Slovenia, the Adriatic grayling (Thymallus thymallus) and marble trout (Salmo marmoratus) following testing different cooling devices and vitrifying media. Sperm was collected, diluted in species-specific non-activating media containing cryoprotectants, and vitrified by plunging directly into liquid nitrogen without pre-cooling. Progressive motility, curvilinear velocity, and straightness of fresh and vitrified-warmed sperm were evaluated with computer-assisted sperm analysis (CASA)...
May 19, 2018: Fish Physiology and Biochemistry
https://www.readbyqxmd.com/read/29778877/an-insight-into-the-multi-scale-structures-and-pasting-behaviors-of-starch-following-citric-acid-treatment
#20
Yinqiang Huo, Binjia Zhang, Meng Niu, Caihua Jia, Siming Zhao, Qilin Huang, Hongying Du
Using combined analytical techniques, this work discloses how citric acid (CA) treatment affects the pasting features of starch from a view of multi-scale structural evolutions. The treatment induced disruption events in starch multi-level structures and thus modulated the pasting behaviors. A structure-property relationship was proposed to rationalize CA effects on starch pasting properties. Specifically, CA disrupted starch structures from molecular scale to micron scale, eventually reducing pasting temperature and paste viscosities...
May 17, 2018: International Journal of Biological Macromolecules
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