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https://www.readbyqxmd.com/read/28109208/psychromonas-aquatilis-sp-nov-isolated-from-water-samples-obtained-in-the-chilean-antarctica
#1
Peter Kämpfer, Rute Irgang, Mathías Poblete-Morales, Stefanie P Glaeser, Marcelo Cortez-San Martín, Ruben Avendaño-Herrera
One slightly beige-white pigmented, Gram-staining negative, rod-shaped bacterium, strain M1A1T, was isolated from seawater samples obtained in Fildes Bay, Antarctica (62º12´S, 58º57´W). Phylogenetic analysis based on nearly full-length 16S rRNA gene sequences showed that the isolate shared 98.4 % 16S rRNA gene sequence identity to the type strain of the species Psychromonas arctica, but less than 97 % 16S rRNA gene sequence similarities to all other Psychromonas species. DNA-DNA hybridisation (DDH) with Psychromonas arctica DSM 14288T showed low values (21 %, reciprocal 27 %)...
January 19, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28109207/rhodohalobacter-halophilus-gen-nov-sp-nov-a-moderately-halophilic-member-of-the-family-balneolaceae
#2
Jun Xia, Zhi-Hong Xie, Christopher A Dunlap, Alejandro P Rooney, Zong-Jun Du
A novel Gram-stain-negative, rod-shaped, facultatively anaerobic, oxidase-negative and catalase-positive bacterium, designated JZ3C29T, was isolated from a saltern located in Feicheng, China. Strain JZ3C29T was tolerant to moderate salt conditions. Optimal growth occurred at 40 °C (range 20-50 °C) and pH 7.5-8.5 (range pH 7.0-9.0) with 8-10 % (w/v) NaCl (range 2-16 %). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain JZ3C29T shared highest similarity with Gracilimonas tropica CL-CB462T (90...
January 19, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28109206/proposal-of-mucilaginibacter-galii-sp-nov-isolated-from-leaves-of-galium-album
#3
Ebru L Aydogan, Hans-Jürgen Busse, Gerald Moser, Christoph Müller, Peter Kämpfer, Stefanie P Glaeser
A pale pink pigmented, Gram-stain-negative, rod-shaped, non-spore forming bacterial strain, PP-F2F-G47T, was isolated from the phyllosphere of the herbaceous plant Galium album. Phylogenetic analysis based on nearly full-length 16S rRNA gene sequence showed highest sequence similarity to type strains of the species Mucilaginibacter daejeonensis (96.2 %), Mucilaginibacter dorajii (95.7 %), and Mucilaginibacter phyllosphaerae (95.5 %). 16S rRNA gene-sequence similarities to all other type strains were below 95...
January 19, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28109204/aquaspirillum-soli-sp-nov-isolated-from-a-soil-sample-in-the-people-s-republic-of-china
#4
Gabriela Moya, Huan Trinh, Zheng-Fei Yan, KyungHwa Won, Jung-Eun Yang, Qi-Jun Wang, MooChang Kook, Tae-Hoo Yi
A Gram-stain-negative, smooth, opaque, white pigmented, helical-shaped and motile bacterium by means of bipolar tufts of flagella, catalase- and oxidase-positive, grew under microaerophilic conditions, was isolated from soil sample of a reed pond in Shangqiu, Henan province, P. R. China. The strain named THG-SQE6T grows well at 25-42 oC, pH 6.5-7.5 and in the presence of 0-1 % (w/v) NaCl. The phylogenetic analysis based on 16S rRNA gene sequence showed that the strain THG-SQE6T was most closely related to Aquaspirillum serpens IAM 13944T (97...
January 19, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28109203/pedobacter-lusitanus-sp-nov-a-new-bacterial-species-isolated-from-sludge-of-a-deactivated-uranium-mine-in-portugal
#5
Cláudia Covas, Tânia Caetano, Andreia Cruz, Tiago Santos, Liliana Dias, Guenter Klein, Amir Abdulmawjood, Luiz Rodríguez-Alcalá, Lígia Pimentel, Ana Gomes, Ana Cristina Freitas, Alba Garcia-Serrano, Javier Fontecha, Sónia Mendo
Strain NL19T is a Gram negative aerobic bacterium that was isolated from sludge of a deactivated uranium mine in Portugal. 16S rRNA gene sequence analysis revealed that strain NL19T is a member of the genus Pedobacter and closely related to the strains Pedobacter himalayensis MTCC 6384T, Pedobacter cryoconitis DSM 14825T, Pedobacter westerhofensis DSM 19036T and P. hartonius DSM 19033T. It has a DNA G+C content of 40.8 mol%, which agreed with the genus description. The main fatty acids include C16:1 9c, C14:1 9c, C4:0, Iso-C17:0, Iso-C17:0 3-OH, C16:0, Anteiso-C15:0 and Iso-C15:0 3-OH...
January 21, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28109202/algoriphagus-resistens-sp-nov-isolated-from-marine-sediment
#6
Ji-Ru Han, Jin-Xin Zhao, Zong-Jie Wang, Guan-Jun Chen, Zong-Jun Du
Strain NH1T, a pink-pigmented, facultatively anaerobic, heterotrophic, catalase-positive and oxidase-negative, Gram-stain-negative marine bacterium, was isolated from marine sediment in the coast of Weihai, China. Cells of strain NH1T was rod-shaped, 0.8-2.0 µm in length and 0.5 µm in width. The strain able to grow at 13-37 °C, pH 5.5-8.5, in the presence of 0.0-8.0% (w/v) NaCl. Optimal growth was observed at 28 °C, with 3.0% (w/v) NaCl and pH 6.5-7.0. Nitrate was reduced. The G+C content of the DNA was 41...
January 19, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28109200/arcobacter-lekithochrous-sp-nov-a-new-species-isolated-from-a-molluscan-hatchery-in-norway
#7
Ana L Diéguez, Sabela Balboa, Thorolf Magnesen, Jesús L Romalde
Four bacterial strains, LFT 1.7T, LT2C 2.5, LT4C 2.8 and TM 4.6, were isolated from Great scallop (Pecten maximus) larvae and tank seawater in a Norwegian hatchery and characterized by a polyphasic approach including phenotypic, chemotaxonomic and genomic traits. All of them were Gram-negative and motile rods, oxidase and catalase positive and required sea salts for growth. Major fatty acids present were Summed Feature 3 (C16:1ω7c/C16:1 ω6c), Summed Feature 8 (C18:1ω7c or C18:1 ω6c), C16:0, C14:0, Summed Feature 2 (C14:0 3OH/C16:1 iso I), C12:0 3OH and C12:0...
January 19, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28109166/a-novel-dual-amylin-and-calcitonin-receptor-agonist-dacra-kbp-089-induces-weight-loss-through-a-reduction-in-fat-but-not-lean-mass-while-improving-food-preference
#8
Sofie Gydesen, Sara Toftegaard Hjuler, Zenia Freving, Kim Vietz Andreassen, Nina Sonne, Lars I Hellgren, Morten Asser Karsdal, Kim Henriksen
BACKGROUND AND PURPOSE: Obesity and associated co-morbidities, such as type 2 diabetes and non-alcoholic fatty liver disease, are major health challenges - hence, development of weight loss therapies with the ability to reduce the co-morbidities is key. EXPERIMENTAL APPROACH: The effect of the dual amylin and calcitonin receptor agonist (DACRA), KBP-089, on bodyweight, glucose homeostasis, and fatty acid accumulation in liver and muscle tissue, food preference was investigated...
January 21, 2017: British Journal of Pharmacology
https://www.readbyqxmd.com/read/28109017/clostridium-butyricum-b1-alleviates-high-fat-diet-induced-steatohepatitis-in-mice-via-enterohepatic-immunoregulation
#9
Da Zhou, Qin Pan, Xiao-Lin Liu, Rui-Xu Yang, Yuan-Wen Chen, Chang Liu, Jian-Gao Fan
BACKGROUND AND AIM: Enterohepatic immunologic derangement is associated with nonalcoholic steatohepatitis (NASH). Here, we investigated whether Clostridium butyricum B1 (CB) would be an effective immune-targeted substance to attenuate steatohepatitis in mice. METHODS: Thirty mice were randomized into a control group fed with common forage, a high-fat diet (HFD) group fed a HFD for 16 weeks, and a HFD + CB group treated with CB for the latter 8 weeks. Inflammation- or metabolism-associated genes in the liver or epididymal fat tissue were quantified, intrahepatic and intestinal immune factors were detected...
January 20, 2017: Journal of Gastroenterology and Hepatology
https://www.readbyqxmd.com/read/28108749/deinococcus-malanensis-sp-nov-isolated-from-radiation-polluted-soil
#10
Jing Zhu, Shan-Hui Li, Qi-Yong Tang, Min Chu, Wei Wang, Nimaichand Salam, Li Li, Wael N Hozzein, Zhi-Dong Zhang, Wen-Jun Li
A Gram-staining positive, non-spore forming, short rod-shaped and coccus-shaped, non-motile, pink-colored, gamma- and UV-resistant strain, designated T93(T) was isolated from soil of Malan area in Xinjiang Uyghur Autonomous Region, Northwest China. The taxonomic position of the new isolate was determined using a polyphasic approach. Strain T93(T) shared the highest 16 S rRNA gene sequence similarity with Deinococcus deserti VCD115(T) (97.54%). The genomic DNA G+C content of the isolate T93(T) was 61.7 mol%...
January 20, 2017: Archives of Microbiology
https://www.readbyqxmd.com/read/28108729/comparative-omega-3-fatty-acid-enrichment-of-egg-yolks-from-first-cycle-laying-hens-fed-flaxseed-oil-or-ground-flaxseed
#11
I J Ehr, M E Persia, E A Bobeck
When laying hen diets are enriched with omega-3 polyunsaturated fatty acids to generate value-added eggs for human consumption markets, concentrations of alpha-linolenic (ALA), eicosapentaenoic (EPA), and docosahexaenoic acids (DHA) in the yolk can reach 250 mg/50 g whole egg. Flaxseed, a rich source of ALA, is commonly used for omega-3 enrichment; however, the impact of dietary flaxseed source (extracted oil vs. milled seed) on fatty acid transfer to egg yolk in laying hens is unknown. Therefore, transfer of ALA, EPA, and DHA into egg yolk from extracted flaxseed oil or milled flaxseed was evaluated in Hy-Line W-36 laying hens over an 8-week feeding period (25 to 33 wk old)...
January 20, 2017: Poultry Science
https://www.readbyqxmd.com/read/28108698/genome-wide-analysis-of-fatty-acid-desaturation-and-its-response-to-temperature
#12
Guillaume Menard, Jose Martin Moreno, Fiona Bryant, Olaya Munoz-Azcarate, Amélie A Kelly, Keywan Hassani-Pak, Smita Kurup, Peter J Eastmond
Plants modify the polyunsaturated fatty acid content of their membrane and storage lipids in order to adapt to changes in temperature. In developing seeds this response is largely controlled by the activities of the microsomal ω-6 and ω-3 fatty acid desaturases, FAD2 and FAD3. Although temperature-regulation of desaturation has been studied at the molecular and biochemical level, the genetic control of this trait is poorly understood. Here we have characterised the response of Arabidopsis thaliana seed lipids to variation in ambient temperature and found that heat inhibits both ω-6 and ω-3 desaturation in phosphatidylcholine, leading to a proportional change in triacylglycerol composition...
January 20, 2017: Plant Physiology
https://www.readbyqxmd.com/read/28108665/fasiglifam-tak-875-alters-bile-acid-homeostasis-in-rats-and-dogs-a-potential-cause-of-drug-induced-liver-injury
#13
Francis S Wolenski, Andy Z X Zhu, Mike Johnson, Shaoxia Yu, Yuu Moriya, Takuya Ebihara, Vilmos Csizmadia, Jessica Grieves, Martin Paton, Mingxiang Liao, Christopher Gemski, Liping Pan, Majid Vakilynejad, Yvonne P Dragan, Swapan K Chowdhury, Patrick J Kirby
Fasiglifam (TAK-875), a Free Fatty Acid Receptor 1 (FFAR1) agonist in development for the treatment of type 2 diabetes, was voluntarily terminated in phase 3 due to adverse liver effects. A mechanistic investigation described in this manuscript focused on the inhibition of bile acid (BA) transporters as a driver of the liver findings. TAK-875 was an in vitro inhibitor of multiple influx (NTCP and OATPs) and efflux (BSEP and MRPs) hepatobiliary BA transporters at micromolar concentrations. Repeat dose studies determined that TAK-875 caused a dose-dependent increase in serum total BA in rats and dogs...
January 20, 2017: Toxicological Sciences: An Official Journal of the Society of Toxicology
https://www.readbyqxmd.com/read/28108554/genome-wide-analysis-of-lncrna-and-mrna-expression-during-differentiation-of-abdominal-preadipocytes-in-the-chicken
#14
Tao Zhang, Xiangqian Zhang, Kunpeng Han, Genxi Zhang, Jinyu Wang, Kaizhou Xie, Qian Xue
Long non-coding RNAs (lncRNAs) regulate adipogenesis and other processes associated with metabolic tissue development and function. However, little is known about the function and profile of lncRNAs during preadipocyte differentiation in the chicken (Gallus gallus). Herein, lncRNA and mRNA expression in preadipocytes at different stages of differentiation were analyzed using RNA sequencing. A total of 1,300,074,528 clean reads and 27,023 novel lncRNAs were obtained from 12 samples. The number of genes (1,336 lncRNAs and 1,759 mRNAs; 3,095 in total) differentially expressed across various stages declined as differentiation progressed...
January 20, 2017: G3: Genes—Genomes—Genetics
https://www.readbyqxmd.com/read/28108519/survival-of-acute-monocytic-leukemia-cells-is-driven-by-fatty-acid-oxidation-mediated-activation-of-ampk-in-bone-marrow-adipocytes
#15
Yoko Tabe, Shinichi Yamamoto, Kaori Saitoh, Kazumasa Sekihara, Norikazu Monma, Kazuho Ikeo, Kaoru Mogushi, Masato Shikami, Vivian R Ruvolo, Jo Ishizawa, Numsen Hail, Saiko Kazuno, Mamoru Igarashi, Hiromichi Matsushita, Yasunari Yamanaka, Hajime Arai, Isao Nagaoka, Takashi Miida, Yoshihide Hayashizaki, Marina Konopleva, Michael Andreeff
Leukemia cells in the bone marrow (BM) must meet the biochemical demands of increased cell proliferation by continually adapting to fluctuations in nutrient and oxygen availability. Thus, targeting metabolic abnormalities in leukemia cells located in the BM may provide an effective therapeutic strategy. In this study, we report the discovery of a metabolic role for BM adipocytes in supporting the growth of leukemic blasts. Preventing nutrient starvation-induced apoptosis of leukemic cells by BM adipocytes, as well as the metabolic and molecular mechanisms involved in this process, were investigated using various analytical techniques...
January 20, 2017: Cancer Research
https://www.readbyqxmd.com/read/28108381/metabolomic-study-of-the-intervention-effects-of-shuihonghuazi-formula-a-traditional-chinese-medicinal-formulae-on-hepatocellular-carcinoma-hcc-rats-using-performance-hplc-esi-tof-ms
#16
Yongrui Bao, Shuai Wang, Xinxin Yang, Li Tianjiao, Mingyue Xia, Xiansheng Meng
ETHNOPHARMACOLOGICAL RELEVANCE: Metabolomics is the comprehensive assessment of endogenous metabolites of a biological system in a holistic context, and its property consists with the global view of Traditional Chinese Medicine (TCM). Shuihonghuazi Formula (SHHZF) has been used for liver cancer early treatment in clinical for more than thirty years, but its mechanism remains unclear completely. This paper was designed to explore the therapeutic effects of SHHZF on liver cancer and its metabolomic characters...
January 17, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/28108339/transcriptome-analysis-of-the-effect-of-polyunsaturated-fatty-acids-against-vibrio-vulnificus-infection-in-oreochromis-niloticus
#17
Chieh-Yu Pan, Yu-Hao Liu, Hong-Yi Gong, Jyh-Yih Chen
Vibrio vulnificus infection causes severe economic losses in Oreochromis niloticus aquaculture by inducing pro-inflammatory cytokines, that lead to inflammation and mortality. Omega-3 fatty acids, such as Docosahexaenoic acid (DHA) and Eicosapentaenoic acid (EPA), have been reported for their anti-inflammatory and antibacterial abilities in murine and zebrafish models. However, the anti-inflammatory and antibacterial functions of DHA and EPA in commercial aquaculture organisms such as Oreochromis niloticus remain unknown...
January 17, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28108329/exercise-increases-mitochondrial-complex-i-activity-and-drp1-expression-in-the-brains-of-aged-mice
#18
Aaron M Gusdon, Jason Callio, Giovanna DiStefano, Robert M O'Doherty, Bret H Goodpaster, Paul M Coen, Charleen T Chu
Exercise is known to have numerous beneficial effects. Recent studies indicate that exercise improves mitochondrial energetics not only in skeletal muscle but also in other tissues. While exercise elicits positive effects on memory, neurogenesis, and synaptic plasticity, the effects of exercise on brain mitochondrial energetics remain relatively unknown. Herein, we studied the effects of exercise training in old and young mice on brain mitochondrial energetics, in comparison to known effects on peripheral tissues that utilize fatty acid oxidation...
January 17, 2017: Experimental Gerontology
https://www.readbyqxmd.com/read/28108102/effects-of-soybean-lipid-infusion-on-unbound-free-fatty-acids-and-unbound-bilirubin-in-preterm-infants
#19
Thomas Hegyi, Alan Kleinfeld, Andrew Huber, Barry Weinberger, Naureen Memon, Weichung Joe Shih, Mary Carayannopoulos, William Oh
OBJECTIVE: To assess the effects of a soybean lipid emulsion infusions on levels of unbound (free) bilirubin (Bf) and unbound free fatty acids (FFAu) as well as changes in Bf and total serum bilirubin (TSB) during phototherapy in infants born preterm. STUDY DESIGN: Ninety-seven infants born preterm (birth weight: 500-2000 g; gestational age: 23-34 weeks) were enrolled to investigate the effect of 0, 1, 2, and 3 g/kg/d of intralipid infusion on Bf and FFAu. Pre- and postphototherapy TSB, FFAu, and Bf also were analyzed in 91 infants to assess the effects of phototherapy...
January 12, 2017: Journal of Pediatrics
https://www.readbyqxmd.com/read/28108057/bani-polymorphism-of-cytosolic-phospholipase-a2-gene-and-somatic-symptoms-in-medication-free-acute-depressed-patients
#20
Jane Pei-Chen Chang, Ta-Wei Guu, Yi-Chih Chen, Piotr Gałecki, Anna Walczewska, Kuan-Pin Su
Somatic symptoms are commonly seen in patients with major depressive disorder (MDD) and might be associated with inflammatory activation. Cytosolic phospholipase A2 (cPLA2) and cyclo-oxygenase-2 (COX-2) are the key enzymes in the metabolism of polyunsaturated fatty acids (PUFAs), which in turn may play an important role in inflammation and somatic symptoms in depression. This study investigated the effects of BanI polymorphism of cPLA2 gene and COX-2 rs4648308 genotypes on somatic symptoms and inflammatory marker in patients with MDD...
January 4, 2017: Prostaglandins, Leukotrienes, and Essential Fatty Acids
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