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https://www.readbyqxmd.com/read/28535613/expression-and-purification-of-biologically-active-human-bone-morphogenetic-protein-4-in-recombinant-chinese-hamster-ovary-cells
#1
Minyub Cha, Nara Han, Jia Pi, Yongsu Jeong, Kwanghee Baek, Jaeseung Yoon
Bone morphogenetic protein-4 (BMP-4) is considered to have therapeutic potential for various diseases including cancers; however, the high expression of biologically active recombinant human BMP-4 (rhBMP-4) needed to manufacture rhBMP-4 for therapeutic purposes has yet to be established. In the current study, we established a recombinant Chinese hamster ovary (rCHO) cell line overexpressing rhBMP-4 as well as a production process using 7.5-l bioreactor (5 l working volume). The expression of the mature rhBMP-4 was significantly enhanced by recombinant furin expression...
May 24, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28535612/efficient-whole-cell-biocatalyst-for-2-3-butanediol-acetoin-production-with-nadh-nad-regeneration-system-in-engineered-serratia-marcescens
#2
Rao Ben, Truong Ngoc Tu, Fan Ji Ying, Sun Jian An, Yaling Shen
Serratia marcescens is reported to possess the potential for industrial 2,3-butanediol or acetoin production by fermentation. But 2,3-butanediol or acetoin are always co-produced and this may make purification process difficult. So biocatalytic technologies may be the appropriate production methods. In this study, we developed an auto-inducing expression system based on pET system and swr quorum sensing system by using S. marcescens. By using this system, S. marcescens could be engineered as whole-cell biocatalyst to obtain 2,3-butanediol or acetoin...
May 24, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28535606/isolation-purification-and-characterization-of-five-active-diketopiperazine-derivatives-from-endophytic-streptomyces-suk-25-with-antimicrobial-and-cytotoxic-activities
#3
Muhanna M Alshaibani, Juriyati Jalil, Nik M Sidik, Siti Junaidah Ahmad, Nurkhalida Kamal, Ruangelie Edrada-Ebel, Noraziah M Zin
On our search for new sources of bioactive secondary metabolites from Streptomyces sp., the ethyl acetate extracts from the endophytic Streptomyces SUK 25 afforded five active diketopiperazines compounds. The aim of this study was to characterize the bioactive compounds isolated from endophytic Streptomyces SUK 25 and evaluate its bioactivity against multiple drug resistance (MDR) bacteria such as Enterococcus raffinosus, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumanii, Pseudomonas aeruginosa and Enterobacter spp...
May 24, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28535479/adsorption-of-vocs-onto-engineered-carbon-materials-a-review
#4
REVIEW
Xueyang Zhang, Bin Gao, Anne Elise Creamer, Chengcheng Cao, Yuncong Li
Volatile organic compounds (VOCs) severely threaten human health and the ecological environment because most of them are toxic, mutagenic, and carcinogenic. The persistent increase of VOCs together with the stringent regulations make the reduction of VOC emissions more imperative. Up to now, numerous VOC treatment technologies have emerged, such as incineration, condensation, biological degradation, absorption, adsorption, and catalysis oxidation et al. Among them, the adsorption technology has been recognized as an efficient and economical control strategy because it has the potential to recover and reuse both adsorbent and adsorbate...
May 12, 2017: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/28535130/home-manufacture-of-drugs-an-online-investigation-and-a-toxicological-reality-check-of-online-discussions-on-drug-chemistry
#5
Evelyn Hearne, Emanuele Amorim Alves, Marie Claire Van Hout, Jean-Paul C Grund
Emerging trends in market dynamics and the use of new psychoactive substances are both a public health concern and a complex regulatory issue. One novel area of investigation is the availability of homemade opioids, amphetamines and dissociatives, and the potential fueling of interest in clandestine home manufacture of drugs via the Internet. We illustrate here how online communal folk pharmacology of homemade drugs on drug website forums may actually inform home manufacture practices or contribute to the reduction of harms associated with this practice...
May 23, 2017: Journal of Psychoactive Drugs
https://www.readbyqxmd.com/read/28534915/quantum-dot-based-sensitive-detection-of-disease-specific-exosome-in-serum
#6
Kseniia Boriachek, Md Nazmul Islam, Vinod Gopalan, Alfred K Lam, Nam-Trung Nguyen, Muhammad J A Shiddiky
Tumor-derived exosomes have emerged as promising cancer biomarkers due to their unique composition and functions. Herein, we report a stripping voltammetric immunoassay for the electrochemical detection of disease-specific exosomes using quantum dots as signal amplifiers. The assay involves three subsequent steps where bulk exosome populations are initially magnetically captured on magnetic beads by a generic tetraspanin antibody (e.g., CD9 or CD63) followed by the identification of disease-specific exosomes using cancer-related...
May 23, 2017: Analyst
https://www.readbyqxmd.com/read/28534782/reinforce-an-ensemble-approach-for-inferring-ppi-network-from-ap-ms-data
#7
Bo Tian, Qiong Duan, Can Zhao, Ben Teng, Zengyou He
Affinity Purification-Mass Spectrometry (AP-MS) is one of the most important technologies for constructing protein-protein interaction (PPI) networks. In this paper, we propose an ensemble method, Reinforce, for inferring PPI network from AP-MS data set. The new algorithm named Reinforce is based on rank aggregation and false discovery rate control. Under the null hypothesis that the interaction scores from different scoring methods are randomly generated, Reinforce follows three steps to integrate multiple ranking results from different algorithms or different data sets...
May 17, 2017: IEEE/ACM Transactions on Computational Biology and Bioinformatics
https://www.readbyqxmd.com/read/28534518/cdk20-interacts-with-keap1-to-activate-nrf2-and-promotes-radiochemoresistance-in-lung-cancer-cells
#8
Q Wang, J Ma, Y Lu, S Zhang, J Huang, J Chen, J-X Bei, K Yang, G Wu, K Huang, J Chen, S Xu
Radiochemoresistance is considered the main cause of local recurrence and distant metastasis in lung cancer. However, the underlying mechanisms of radiochemoresistance remain to be uncovered. In this study, we determine the functions of cell cycle-related kinase (CDK20) in radiochemoresistance. CDK20 is a newly identified protein kinase, which plays critical roles in cell growth and proliferation in several types of cancer. Using tandem affinity purification technology, we provide evidences that CDK20 binds to the ubiquitin ligase Kelch-like ECH-associated protein 1 (KEAP1), which targets transcriptional factor nuclear factor erythroid-2-related factor 2 (NRF2) for degradation...
May 22, 2017: Oncogene
https://www.readbyqxmd.com/read/28534479/efficient-protein-production-inspired-by-how-spiders-make-silk
#9
Nina Kronqvist, Médoune Sarr, Anton Lindqvist, Kerstin Nordling, Martins Otikovs, Luca Venturi, Barbara Pioselli, Pasi Purhonen, Michael Landreh, Henrik Biverstål, Zigmantas Toleikis, Lisa Sjöberg, Carol V Robinson, Nicola Pelizzi, Hans Jörnvall, Hans Hebert, Kristaps Jaudzems, Tore Curstedt, Anna Rising, Jan Johansson
Membrane proteins are targets of most available pharmaceuticals, but they are difficult to produce recombinantly, like many other aggregation-prone proteins. Spiders can produce silk proteins at huge concentrations by sequestering their aggregation-prone regions in micellar structures, where the very soluble N-terminal domain (NT) forms the shell. We hypothesize that fusion to NT could similarly solubilize non-spidroin proteins, and design a charge-reversed mutant (NT*) that is pH insensitive, stabilized and hypersoluble compared to wild-type NT...
May 23, 2017: Nature Communications
https://www.readbyqxmd.com/read/28534126/optimization-of-continuous-flow-solid-phase-denitrification-via-coupling-carriers-in-enhancing-simultaneous-removal-of-nitrogen-and-organics-for-agricultural-runoff-purification
#10
Yi Lu, Xiaoqing Zhang, Lijuan Feng, Guangfeng Yang, Zhou Zheng, Junzhi Liu, Jun Mu
Coupling of biodegradable corncob and plastic carrier was optimized in continuous-flow solid-phase denitrification systems for enhancing simultaneously removal of nitrogen and organics in agricultural runoff. In compared with preposition of plastic carriers and mixed distribution method, it was demonstrated that the preposition of corncobs simultaneously enhanced nitrate (6.64 ± 1.35 mg L(-1) day (-1)) and organics removal (6.33 ± 1.44 mg L(-1) day(-1)) at a hydraulic retention time (HRT) of 6 h...
May 22, 2017: Biodegradation
https://www.readbyqxmd.com/read/28533942/hla-a24-ligandome-analysis-of-colon-and-lung-cancer-cells-identifies-a-novel-cancer-testis-antigen-and-a-neoantigen-that-elicits-specific-and-strong-ctl-responses
#11
Vitaly Kochin, Takayuki Kanaseki, Serina Tokita, Sho Miyamoto, Yosuke Shionoya, Yasuhiro Kikuchi, Daichi Morooka, Yoshihiko Hirohashi, Tomohide Tsukahara, Kazue Watanabe, Shingo Toji, Yasuo Kokai, Noriyuki Sato, Toshihiko Torigoe
This study focused on HLA-A24 and comprehensively analyzed the ligandome of colon and lung cancer cells without the use of MHC-binding in silico prediction algorithms. Affinity purification using the antibody specific to HLA-A24 followed by LC-MS/MS sequencing was used to detect peptides, which harbored the known characteristics of HLA-A24 peptides in terms of length and anchor motifs. Ligandome analysis demonstrated the natural presentation of two different types of novel tumor-associated antigens. The ligandome contained a peptide derived from SUV39H2, a gene found to be expressed in a variety of cancers but not in normal tissues (except for the testis)...
2017: Oncoimmunology
https://www.readbyqxmd.com/read/28532216/structural-insights-into-the-eukaryotic-transcription-initiation-machinery
#12
Eva Nogales, Robert K Louder, Yuan He
Eukaryotic gene transcription requires the assembly at the promoter of a large preinitiation complex (PIC) that includes RNA polymerase II (Pol II) and the general transcription factors TFIID, TFIIA, TFIIB, TFIIF, TFIIE, and TFIIH. The size and complexity of Pol II, TFIID, and TFIIH have precluded their reconstitution from heterologous systems, and purification relies on scarce endogenous sources. Together with their conformational flexibility and the transient nature of their interactions, these limitations had precluded structural characterization of the PIC...
May 22, 2017: Annual Review of Biophysics
https://www.readbyqxmd.com/read/28532127/investigation-of-free-standing-plasmonic-mesoporous-ag-cmk-8-nafion-composite-membrane-for-the-removal-of-organic-pollutants-with-254-nm-uv-irradiation
#13
Chuan Ming Tseng, Hsin Liang Chen, Sz Nian Lai, Ming Shiung Chen, Chien Jung Peng, Chia Jui Li, Wei Hsuan Hung
"Carbon-based material" has demonstrated a great potential on water purification due to its strong physical adsorption to organic pollutants in the water. Three-dimensional cubic ordered mesoporous carbon (CMK-8), one of the well-known ordered mesoporous carbons, was prepared by using nanocasting method with mesoporous silica (KIT-6) as the template. In this study, CMK-8 blended with Nafion polymer to form a free-standing mesoporous CMK-8-Nafion composite membrane. The synthesis of high crystallinity CMK-8 was characterized by X-ray diffraction (XRD) and transmission electron microscopy (TEM)...
December 2017: Nanoscale Research Letters
https://www.readbyqxmd.com/read/28532053/functionalized-fe3o4-graphene-oxide-nanocomposites-with-hairpin-aptamers-for-the-separation-and-preconcentration-of-trace-pb-2-from-biological-samples-prior-to-determination-by-icp-ms
#14
Mojtaba Shamsipur, Leila Farzin, Mahmoud Amouzadeh Tabrizi, Shahab Sheibani
Lead (Pb) as a topically poisonous metal represents a serious threat to the ecological environment and especially to human beings. Therefore, it is urgent to develop a rapid and reliable monitoring technique for this heavy metal in the environmental samples. In the present study, we have designed a selective and sensitive method for the determination of ultratrace contents of Pb(2+) in biological samples, based on the guanine (G)-quadruplex formed by the aptamer with hairpin structure and Pb(2+). For this purpose, Pb(2+) specific aptamer serving as affinity probe to capture and separate trace amounts of the analyte, was covalently linked to Fe3O4/graphene oxide (GO) surface by using a suitable cross-linking agent...
August 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28531968/retention-of-metal-and-sulphate-ions-from-acidic-mining-water-by-anionic-nanofibrillated-cellulose
#15
Salla H Venäläinen, Helinä Hartikainen
We carried out an adsorption experiment to investigate the ability of anionic nanofibrillated cellulose (NFC) to retain metal and SO4(2-) ions from authentic highly acidic (pH3.2) mining water. Anionic NFC gels of different consistencies (1.1-%, 1.4-% and 1.8-% w/w) were allowed to react for 10min with mining water, after which NFC-induced changes in the metal and SO4(2-) concentrations of the mining water were determined. The sorption capacities of the NFC gels were calculated as the difference between the element concentrations in the untreated and NFC-treated mining water samples...
May 18, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28531912/from-bench-to-market-preparing-human-pluripotent-stem-cells-derived-cardiomyocytes-for-various-applications
#16
REVIEW
Sung-Hwan Moon, Daekyeong Bae, Taek-Hee Jung, Eun-Bin Chung, Young-Hoon Jeong, Soon-Jung Park, Hyung-Min Chung
Human cardiomyocytes (CMs) cease to proliferate and remain terminally differentiated thereafter, when humans reach the mid-20s. Thus, any damages sustained by myocardium tissue are irreversible, and they require medical interventions to regain functionality. To date, new surgical procedures and drugs have been developed, albeit with limited success, to treat various heart diseases including myocardial infarction. Hence, there is a pressing need to develop more effective treatment methods to address the increasing mortality rate of the heart diseases...
May 30, 2017: International Journal of Stem Cells
https://www.readbyqxmd.com/read/28531457/new-structural-insights-into-the-oligosaccharide-phosphate-fraction-of-pichia-hansenula-holstii-nrrl-y2448-phosphomannan
#17
Paul N Handley, Anthony Carroll, Vito Ferro
The oligosaccharide phosphate fraction (OPF) obtained from mild acid hydrolysis of P. holstii NRRL Y-2448 phosphomannan is the starting material for the preparation of the Phase III anticancer drug candidate PI-88. The OPF was for the first time successfully separated by preparative ion exchange chromatography and the major oligosaccharides isolated and characterized by NMR spectroscopy. The components were also acetylated and subjected to LC-MS analysis. These studies revealed that the OPF also contained all-α(1 → 3)-linked oligosaccharides in addition to the known α(1 → 3)/(1 → 2)-linked species, most likely formed by hydrolysis of the latter...
May 13, 2017: Carbohydrate Research
https://www.readbyqxmd.com/read/28530199/synthesis-of-ag-nps-impregnated-cellulose-composite-material-its-possible-role-in-wound-healing-and-photocatalysis
#18
Attarad Ali, Ihsan Ul Haq, Javeed Akhtar, Muhammad Sher, Naveed Ahmed, Muhammad Zia
Cellulose is the natural biopolymer normally used as supporting agent with enhanced applicability and properties. In present study, cellulose isolated from citrus waste is used for silver nanoparticles (Ag-NPs) impregnation by a simple and reproducible method. The Ag-NPs fabricated cellulose (Ag-Cel) was characterised by powder X-rays diffraction, Fortier transform infrared spectroscopy and scanning electron microscopy. The thermal stability was studied by thermo-gravimetric analysis. The antibacterial activity performed by disc diffusion assay reveals good zone of inhibition against Staphylococcus aureus and Escherichia coli by Ag-Cel as compared Ag-NPs...
June 2017: IET Nanobiotechnology
https://www.readbyqxmd.com/read/28529978/emerging-applications-of-exosomes-in-cancer-therapeutics-and-diagnostics
#19
REVIEW
Sahil Inamdar, Rajeshwar Nitiyanandan, Kaushal Rege
Exosomes are nanoscale extracellular vesicles that are shed from different cells in the body. Exosomes encapsulate several biomolecules including lipids, proteins, and nucleic acids, and can therefore play a key role in cellular communication. These vesicles can be isolated from different body fluids and their small sizes make them attractive in various biomedical applications. Here, we review state-of-the art approaches in exosome isolation and purification, and describe their potential use in cancer vaccines, drug delivery, and diagnostics...
March 2017: Bioengineering & Translational Medicine
https://www.readbyqxmd.com/read/28529672/on-chip-density-based-purification-of-liposomes
#20
Siddharth Deshpande, Anthony Birnie, Cees Dekker
Due to their cell membrane-mimicking properties, liposomes have served as a versatile research tool in science, from membrane biophysics and drug delivery systems to bottom-up synthetic cells. We recently reported a novel microfluidic method, Octanol-assisted Liposome Assembly (OLA), to form cell-sized, monodisperse, unilamellar liposomes with excellent encapsulation efficiency. Although OLA provides crucial advantages over alternative methods, it suffers from the presence of 1-octanol droplets, an inevitable by-product of the production process...
May 2017: Biomicrofluidics
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