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https://www.readbyqxmd.com/read/29161268/nucleic-acid-purification-from-plants-animals-and-microbes-in-under-30-seconds
#1
Yiping Zou, Michael Glenn Mason, Yuling Wang, Eugene Wee, Conny Turni, Patrick J Blackall, Matt Trau, Jose Ramon Botella
Nucleic acid amplification is a powerful molecular biology tool, although its use outside the modern laboratory environment is limited due to the relatively cumbersome methods required to extract nucleic acids from biological samples. To address this issue, we investigated a variety of materials for their suitability for nucleic acid capture and purification. We report here that untreated cellulose-based paper can rapidly capture nucleic acids within seconds and retain them during a single washing step, while contaminants present in complex biological samples are quickly removed...
November 2017: PLoS Biology
https://www.readbyqxmd.com/read/29161226/development-of-competitive-enzyme-linked-immunosorbent-assays-for-antibody-detection-based-on-bluetongue-virus-monoclonal-antibodies
#2
Zhou Han, Zang Mingxin, Li Xuechun, Xu Yigang, Qiao Xinyuan, Wang Li, Cui Wen, Jiang Yanping, Li Yijing, Tang Lijie
Bluetongue is a ruminant infectious disease that is characterized by hyperpyrexia, leukocyte decrease and mucosal ulcerative inflammation changes. In this study, three segments of Bluetongue virus (BTV)-8 VP2 protein (BTV-8A, 8B, and 8C) were cloned into pET-28a (+) and pMAl-c5X vectors and expressed in Escherichia coli BL21 (DE3) as histidine (His)-tagged (His-8A/8B/8C) and maltose-binding protein (MBP)-tagged (MBP-8A/8B/8C) fusion proteins. After purification, His-8A/8B/8C were used to immunize BALB/mice and MBP-8A/8B/8C were used to screen for monoclonal antibody (mAb)-secreting hybridomas...
November 21, 2017: Viral Immunology
https://www.readbyqxmd.com/read/29160716/-and-ecarlottones-uncommon-chalconoids-from-fissistigma-latifolium-with-pro-apoptotic-activity
#3
Charlotte Gény, Alma Abou Samra, Pascal Retailleau, Bogdan I Iorga, Hristo Nedev, Khalijah Awang, Fanny Roussi, Marc Litaudon, Vincent Dumontet
Four new compounds, (+)- and (-)-ecarlottone (1), (±)-fislatifolione (5), (±)-isofislatifolione (6), and (±)-fislatifolic acid (7), and the known desmethoxyyangonin (2), didymocarpin-A (3), and dehydrodidymocarpin-A (4) were isolated from the stem bark of Fissistigma latifolium, by means of bioassay-guided purification using an in vitro affinity displacement assay based on the modulation of Bcl-xL/Bak and Mcl-1/Bid interactions. The structures of the new compounds were elucidated by NMR spectroscopic data analysis, and the absolute configurations of compounds (+)-1 and (-)-1 were assigned by comparison of experimental and computed ECD spectra...
November 21, 2017: Journal of Natural Products
https://www.readbyqxmd.com/read/29160698/functional-characterization-of-salicylic-acid-carboxyl-methyltransferase-from-camellia-sinensis-providing-the-aroma-compound-of-methyl-salicylate-during-withering-process-of-white-tea
#4
Wei-Wei Deng, Rongxiu Wang, Tianyuan Yang, Li'na Jiang, Zhengzhu Zhang
Methyl salicylate (MeSA) is one of the volatile organic compounds (VOCs) that releases floral scent, and plays an important role in tea sweet flowery aroma. During the withering process for white tea producing, MeSA was generated by salicylic acid carboxyl methyltransferase (SAMT) with salicylic acid (SA), and the specific floral scent was formed. In this study, we firstly cloned a CsSAMT from tea leaves (GenBank accession No. MG459470) and used Escherichia coli and Saccharomyces cerevisiae to express the recombinant CsSAMT...
November 21, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29160181/chick-embryo-model-homing-and-host-interactions-of-tissue-engineering-aimed-human-dental-stem-cells
#5
Jennifer Adriane Dos Santos, Monica Talarico Duailibi, Durvanei Augusto Maria, Sonia Elisabete Alves de Lima Will, Paulo Cesar Simoes Silva, Ligia Ferreira Gomes, Silvio Eduardo Duailibi
Human dental stem cells (hDSC) have a potential for regenerative therapies and could differentiate in vitro into many tissues such as dentin, nerve and vascular endothelium. Gallus gallus domesticus developing fertilized egg, or chick embryo is an experimental model absent of xenografts rejection, largely employed in replacement of mammal species in scientific research and pre-clinical studies to evaluate angiogenesis and vasculogenesis, tissue differentiation, and embryonic development. This multiscale research deals with the homing and cell signaling effects of a standardized hDSC towards the receptor tissues of Gallus gallus domesticus in ovo...
November 21, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/29160016/synthetic-oligosaccharide-libraries-and-microarray-technology-a-powerful-combination-for-the-success-of-the-current-glycosaminoglycan-interactomics
#6
Vitor H Pomin, Xu Wang
Glycosaminoglycans (GAGs) are extracellular matrix and/or cell surface sulfated glycans crucial to the regulation of various signaling proteins whose functions are essential in many pathophysiologycal systems. Because structural heterogeneity is high in GAG chains and purification is difficult, the use of structurally defined GAG oligosaccharides from natural sources as molecular models in both biophysical and pharmacological assays is limited. To overcome this obstacle, GAG-like oligosaccharides of well-defined structures are currently being synthezised by chemical and/or enzymatic means in many laboratories around the world...
November 20, 2017: ChemMedChem
https://www.readbyqxmd.com/read/29159916/purification-and-characterization-of-ribosomal-proteins-l27-and-l30-having-antimicrobial-activity-produced-by-the-lactobacillus-salivarius-sgl03
#7
Paolo Pidutti, Federica Federici, Jessica Brandi, Laura Manna, Eleonora Rizzi, Umberto Marini, Daniela Cecconi
AIMS: The aim of this study was to investigate the antimicrobial potential of proteins secreted by a new strain of Lactobacillus salivarius. METHODS AND RESULTS: The secretome of L. salivarius SGL03 strain was analyzed by gel-assisted fractionation and MS/MS to identify low-molecular-mass proteins. This strategy allowed us to identify ten secreted proteins. Then a combination of heterologous expression and agar well diffusion was used to characterize them as to their antimicrobial activity, mechanisms of action, and stability...
November 21, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/29159657/polysaccharides-from-dolichos-biflorus-linn-and-trachyspermum-ammi-linn-seeds-isolation-characterization-and-remarkable-antimicrobial-activity
#8
Shibani Basu, Manojit Ghosh, Rupam Kumar Bhunia, Jhuma Ganguly, Bimal K Banik
Polysaccharides are structurally complex and essential constituents of life, and therefore, studies directed to these kinds of molecules have received scientific attention. Despite an easy availability of Dolichos biflorus Linn and Trachyspermum ammi (Linn) seeds isolation, characterization and antimicrobial studies of polysaccharides derived from these two natural sources have not been investigated. Therefore, we report here isolation of polysaccharides, their purification and characterization from Dolichos biflorus Linn and Trachyspermum ammi (Linn) seeds...
November 21, 2017: Chemistry Central Journal
https://www.readbyqxmd.com/read/29159220/data-on-atherosclerosis-specific-antibody-conjugation-to-nanoemulsions
#9
Geoffrey Prévot, Martine Duonor-Cérutti, Mélusine Larivière, Jeanny Laroche-Traineau, Marie Josée Jacobin-Valat, Philippe Barthélémy, Gisèle Clofent-Sanchez, Sylvie Crauste-Manciet
This article present data related to the publication entitled "Iron oxide core oil-in-water nanoemulsion as tracer for atherosclerosis MPI and MRI imaging" (Prévot et al., 2017) [1]. Herein we describe the engineering in the baculovirus-insect cell system and purification processes of the human scFv-Fc TEG4-2C antibody, specific of platelets within the atheroma plaque. For molecular targeting purpose, atheroma specific antibody was conjugated to nanoemulsions (NEs) using a heterobifunctional linker (DSPE-PEG-maleimide)...
December 2017: Data in Brief
https://www.readbyqxmd.com/read/29158832/clinical-translation-and-first-in-human-use-of-44-sc-sc-psma-617-for-pet-imaging-of-metastasized-castrate-resistant-prostate-cancer
#10
Elisabeth Eppard, Ana de la Fuente, Martina Benešová, Ambreen Khawar, Ralph A Bundschuh, Florian C Gärtner, Barbara Kreppel, Klaus Kopka, Markus Essler, Frank Rösch
BACKGROUND: Various trivalent radiometals are well suited for labeling of DOTA-conjugated variants of Glu-ureido-based prostate-specific membrane antigen (PSMA) inhibitors. The DOTA-conjugate PSMA-617 has proven high potential in PSMA radioligand therapy (PSMA-RLT) of prostate cancer as well as PET imaging when labeled with lutetium-177 and gallium-68 respectively. Considering the relatively short physical half-life of gallium-68 this positron emitter precludes prolonged acquisition periods, as required for pre-therapeutic dosimetry or intraoperative applications...
2017: Theranostics
https://www.readbyqxmd.com/read/29157907/expression-and-purification-of-plant-fructan-exohydrolases-and-their-potential-applications-in-fructose-production
#11
Wenyue Zhan, Lijin Jin, Jiao Jiao, Xi Zhang, Yan Zhang, Haiyan Zhao, Mingxiang Liang
Inulinases from microorganisms have been extensively studied for their role in the production of fructose from fructan. Fructan can also be hydrolyzed by plant fructan exohydrolases (FEHs), but these enzymes have not been used to produce fructose commercially. Two Ht1-FEHs (Ht1-FEH I and Ht1-FEH II) were recently characterized in Jerusalem artichoke. In this study, we cloned the third member of the Ht1-FEH family in Jerusalem artichoke (i.e., Ht1-FEH III). When heterologously expressed in Pichia pastoris X-33, Ht1-FEH III not only demonstrated hydrolysis activity towards β (2, 1)-linked fructans and β (2, 6)-linked levan, but also towards sucrose...
November 17, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29156758/development-of-novel-long-noncoding-rna-malat1-near-infrared-optical-probes-for-in-vivo-tumour-imaging
#12
Meng-Jie Dong, Cai-Qin Wang, Guo-Lin Wang, Yue-Hong Wang, Zhen-Feng Liu
With the advent of next-generation sequencing technology, there is rapidly increasing interest in long noncoding RNAs (lncRNAs). The objectives of this study were to develop a novel lncRNA MALAT1 near-infrared optical probe, to evaluate the characteristics of this optical imaging probe in vitro and to determine whether it can be used for imaging MALAT1 expression in malignant tumours in vivo. Conjugation of Cy5.5 to MALAT1 ASO was accomplished using standard NHS (N-hydroxysuccinimide) ester procedures, and the labelled MALAT1 ASO was purified with a Glen-Pak DNA Purification Cartridge and reversed-phase high performance liquid chromatography (HPLC)...
October 17, 2017: Oncotarget
https://www.readbyqxmd.com/read/29156652/comparison-of-the-chemical-profiles-and-antioxidant-activities-of-different-parts-of-cultivated-cistanche-deserticola-using-ultra-performance-liquid-chromatography-quadrupole-time-of-flight-mass-spectrometry-and-a-1-1-diphenyl-2-picrylhydrazyl-based-assay
#13
Xiaoming Wang, Jinfang Wang, Huanyu Guan, Rong Xu, Xiaomei Luo, Meifeng Su, Xiaoyan Chang, Wenting Tan, Jun Chen, Yue Shi
In this study, a sensitive ultra-performance liquid chromatography-photodiode array coupled to quadruple time-of-flight mass (UPLC-PDA-Q/TOF-MS) method and a 1,1-diphenyl-2- picrylhydrazyl (DPPH)-based assay were used to determine the chemical constituents and screen the antioxidant activity profiles of the methanol extracts of different parts of cultivated Cistanche deserticola (C. deserticola). First, qualitative and quantitative chemical composition analyses of the different parts of cultivated C. deserticola were conducted...
November 20, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29156578/pancreatic-cancer-molecular-characterization-clonal-evolution-and-cancer-stem-cells
#14
REVIEW
Elvira Pelosi, Germana Castelli, Ugo Testa
Pancreatic Ductal Adenocarcinoma (PDAC) is the fourth most common cause of cancer-related death and is the most lethal of common malignancies with a five-year survival rate of <10%. PDAC arises from different types of non-invasive precursor lesions: intraductal papillary mucinous neoplasms, mucinous cystic neoplasms and pancreatic intraepithelial neoplasia. The genetic landscape of PDAC is characterized by the presence of four frequently-mutated genes: KRAS, CDKN2A, TP53 and SMAD4. The development of mouse models of PDAC has greatly contributed to the understanding of the molecular and cellular mechanisms through which driver genes contribute to pancreatic cancer development...
November 18, 2017: Biomedicines
https://www.readbyqxmd.com/read/29155755/in-vivo-detection-and-analysis-of-rb-protein-sumoylation-in-human-cells
#15
Fengxi Meng, Xiaofeng Li, Hui Ren, Jiang Qian
The post-translational modifications of proteins are critical for the proper regulation of intracellular signal transduction. Among these modifications, small ubiquitin-related modifier (SUMO) is a ubiquitin-like protein that is covalently attached to the lysine residues of a variety of target proteins to regulate cellular processes, such as gene transcription, DNA repair, protein interaction and degradation, subcellular transport, and signal transduction. The most common approach to detecting protein SUMOylation is based on the expression and purification of recombinant tagged proteins in bacteria, allowing for an in vitro biochemical reaction which is simple and suitable for addressing mechanistic questions...
November 2, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29155737/synthesis-and-evaluation-of-a-ruthenium-based-mitochondrial-calcium-uptake-inhibitor
#16
Sarah R Nathan, Justin J Wilson
We detail the synthesis and purification of a mitochondrial calcium uptake inhibitor, [(OH2)(NH3)4Ru(µ-O)Ru(NH3)4(OH2)](5+). The optimized synthesis of this compound commences from [Ru(NH3)5Cl]Cl2 in 1 M NH4OH in a closed container, yielding a green solution. Purification is accomplished with cation-exchange chromatography. This compound is characterized and verified to be pure by UV-vis and IR spectroscopy. The mitochondrial calcium uptake inhibitory properties are assessed in permeabilized HeLa cells by fluorescence spectroscopy...
October 26, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29155468/gram-scale-synthesis-of-the-sparteine-surrogate-and-sparteine
#17
James D Firth, Steven J Canipa, Peter O'Brien, Leigh Ferris
An 8-step, gram-scale synthesis of the (-)-sparteine surrogate (22% yield, with just 3 chromatographic purifications) and a 10-step, gram-scale synthesis of (-)-sparteine (31% yield) are reported. Both syntheses proceed with complete diastereocontrol and allow access to either antipode. Since the syntheses do not rely on natural product extraction, our work addresses long-term supply issues relating to these widely used chiral ligands.
November 20, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/29155263/identification-and-characterisation-of-the-epididymal-proteins-in-the-lizard-eutropis-carinata-reptilia-squamata-schneider-1801
#18
R Medini, M Bhagya, S Samson
Lizards are seasonal breeders. Cyclic reproductive nature makes lizard as a useful model for the study of the reproductively active protein secretions in the epididymis. During breeding season, the epididymides of the lizard secret proteins that mixes with the spermatozoa and create a favourable environment for sperm maturation. In this spectrum, the aim of this study is to identify and characterize proteins which are present in the lumen of the epididymis of the lizard, E. carinata during the active phase of reproduction...
November 16, 2017: General and Comparative Endocrinology
https://www.readbyqxmd.com/read/29155159/purification-characterization-and-anti-tumor-activities-of-polysaccharides-extracted-from-wild-russula-griseocarnosa
#19
Yang Liu, Jinjin Zhang, Zhaoli Meng
The anti-tumor activity of a novel polysaccharide, PRG1-1, obtained from Russula griseocarnosa sporocarp was investigated in this paper. PRG1-1 has a molecular weight of 630kDa and was extracted and purified using DEAE-cellulose and gel filtration chromatography from crude polysaccharide extract of R. griseocarnosa sporocarp. PRG1-1 was composed of glucose, galactose, mannose, xylose and fructose, in a molar ratio of 66.5:29.2:3.17: 0.663:0.447, respectively. Purified PRG1-1 significantly reduced cell viability, increased the production of lactate dehydrogenase (LDH) and reactive oxygen species (ROS), and enhanced the apoptotic rate in HeLa and SiHa cells...
November 15, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29155144/bay-60-2770-activates-two-isoforms-of-nitric-oxide-sensitive-guanylyl-cyclase-evidence-for-stable-insertion-of-activator-drugs
#20
Anne Sömmer, Peter Sandner, Sönke Behrends
Nitric oxide sensitive guanylyl cyclase (NOsGC), a hemoprotein and the major physiological receptor for nitric oxide (NO), is a heterodimer with the α1/β1 and α2/β1 isoforms known to be important for NO-signaling and conversion of GTP to cGMP in humans. Two innovative classes of compounds modulating the NO/cGMP signaling pathway have been discovered: the heme-dependent sGC stimulators, that stimulate NOsGC directly and also increase the affinity towards NO, and the heme-independent sGC activators, that are thought to bind to oxidized and heme-free NOsGC in tissues exposed to oxidative stress...
November 15, 2017: Biochemical Pharmacology
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