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https://www.readbyqxmd.com/read/28445704/development-of-standard-methods-for-zika-virus-propagation-titration-and-purification
#1
Sharton Vinicius Antunes Coelho, Rômulo Leão Silva Neris, Michelle Premazzi Papa, Laila Castro Schnellrath, Lana Monteiro Meuren, Diogo A Tschoeke, Luciana Leomil, Brunno Renato Farias Verçoza, Milene Miranda, Fabiano L Thompson, Andrea Thompson Da Poian, Thiago Moreno L Souza, Fabiana Avila Carneiro, Clarissa R Damaso, Iranaia Assunção-Miranda, Luciana Barros de Arruda
The emergence of Zika virus (ZIKV) infection has stimulated several research groups to study and collaborate to understand virus biology and pathogenesis. These efforts may assist with the development of antiviral drugs, vaccines and diagnostic tests, as well as to promote advancements in public health policies. Here, we aim to develop standard protocols for propagation, titration, and purification of Asian and Brazilian ZIKV strains, by systematically testing different cell types, kinetics, multiplicity of infection and centrifugation protocols...
April 23, 2017: Journal of Virological Methods
https://www.readbyqxmd.com/read/28445630/murai-reaction-on-furfural-derivatives-enabled-by-removable-n-n-bidentate-directing-groups
#2
Giovanni Poli, Julie Oble, Luis Veiros, Cristofer Pezzetta
Furfural and related compounds are industrially relevant building-blocks obtained from lignocellulosic biomass. To enhance the added value of these renewable resources, a Ru-catalyzed hydrofurylation of alkenes, involving a directed C-H activation at C3 of the furan ring, was developed. A thorough experimental study revealed that a bidentate amino-imine directing group enables the desired coupling. Removal of the directing group takes place during the purification step, directly releasing the C3-functionalized furfurals...
April 26, 2017: Chemistry: a European Journal
https://www.readbyqxmd.com/read/28445010/detection-of-serum-antibodies-to-hepatitis-e-virus-based-on-hev-genotype-3-orf2-capsid-protein-expressed-in-nicotiana-benthamiana
#3
Milena Mazalovska, Nikola Varadinov, Tsvetoslav Koynarski, Ivan Minkov, Pavel Teoharov, George P Lomonossoff, Gergana Zahmanova
BACKGROUND: Hepatitis E virus (HEV) causes epidemics in developing countries and is primarily transmitted through the fecal-oral route. There have been recent reports on the zoonotic spread of the virus, and several animal species, primarily pigs, have been recognized as reservoirs of HEV. Because of its possible spread, there is an urgent need of a method for the cost-effective production of HEV proteins that can be used as diagnostic antigens for the serological detection of anti-HEV antibodies...
July 2017: Annals of Laboratory Medicine
https://www.readbyqxmd.com/read/28444801/comprehensive-identification-and-structural-characterization-of-target-components-from-gelsemium-elegans-by-high-performance-liquid-chromatography-coupled-with-quadruopole-time-of-flight-mass-spectrometry-based-on-accurate-mass-databases-combined-with-ms-ms
#4
Yan-Chun Liu, Sa Xiao, Kun Yang, Li Ling, Zhi-Liang Sun, Zhao-Ying Liu
This study reports an applicable analytical strategy of comprehensive identification and structure characterization of target components from Gelsemium elegans by using high-performance liquid chromatography quadruopole time-of-flight mass spectrometry (LC-QqTOF MS) based on the use of accurate mass databases combined with MS/MS spectra. The databases created included accurate masses and elemental compositions of 204 components from Gelsemium and their structural data. The accurate MS and MS/MS spectra were acquired through data-dependent auto MS/MS mode followed by an extraction of the potential compounds from the LC-QqTOF MS raw data of the sample...
April 26, 2017: Journal of Mass Spectrometry: JMS
https://www.readbyqxmd.com/read/28444602/bioactive-guided-fractionation-of-diols-from-streptomyces-sp-msl
#5
Raja Hima Bindhu Madasu, Vijayalakshmi Muvva, Rajesh Kumar Munaganti, Kumar Reddy Dorigondla, Venkateswarlu Yenamandra
An actinomycete strain with a great potential to produce bioactive compounds isolated from a laterite soil was identified as Streptomyces sp. MSL based on 16S rDNA sequence analysis. Secondary metabolites produced by the strain in optimized nutrient broth were extracted and analyzed by chromatographic and spectroscopic techniques. Among the different fractions, four diols, viz., (1) (2R,3R)-2,3-Butanediol, (2) (2R,3S)-2,3-Butanediol, (3) 2,3-dimethyl-2,3-butanediol (Pinacol), and (4) (3R)-1,3-Butanediol exhibited good antimicrobial activity...
May 2017: 3 Biotech
https://www.readbyqxmd.com/read/28444399/development-of-a-multipurpose-scaffold-for-the-display-of-peptide-loops
#6
Maxim Rossmann, Sandra J Greive, Tommaso Moschetti, Michael Dinan, Marko Hyvönen
Protein-protein interactions (PPIs) determine a wide range of biological processes and analysis of these dynamic networks is increasingly becoming a mandatory tool for studying protein function. Using the globular ATPase domain of recombinase RadA as a scaffold, we have developed a peptide display system (RAD display), which allows for the presentation of target peptides, protein domains or full-length proteins and their rapid recombinant production in bacteria. The design of the RAD display system includes differently tagged versions of the scaffold, which allows for flexibility in the protein purification method, and chemical coupling for small molecule labeling or surface immobilization...
April 24, 2017: Protein Engineering, Design & Selection: PEDS
https://www.readbyqxmd.com/read/28443917/hydrogen-purification-performance-of-a-nanoporous-hexagonal-boron-nitride-membrane-molecular-dynamics-and-first-principle-simulations
#7
Masoud Darvish Ganji, Razieh Dodangeh
Membranes have attracted much attention for the efficient separation of gas mixtures, due to their specific structural and unique properties. In this work, density functional theory (DFT) and molecular dynamic (MD) simulations have been employed to evaluate the performance of nanoporous hexagonal boron nitride (h-BN) monolayers for hydrogen purification. Various porous membranes were designed, and full structural relaxation was carried out by using DFT calculations and then MD simulations to investigate the H2 purification performance of the nanoporous h-BN membranes...
April 26, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28443841/automatic-concentration-and-reformulation-of-pet-tracers-via-microfluidic-membrane-distillation
#8
Philip H Chao, Jeffery Collins, Joseph P Argus, Wei-Yu Tseng, Jason T Lee, R Michael van Dam
Short-lived radiolabeled tracers for positron emission tomography (PET) must be rapidly synthesized, purified, and formulated into injectable solution just prior to imaging. Current radiosynthesizers are generally designed for clinical use, and the HPLC purification and SPE formulation processes often result in a final volume that is too large for preclinical and emerging in vitro applications. Conventional technologies and techniques for reducing this volume tend to be slow, resulting in radioactive decay of the product, and often require manual handling of the radioactive materials...
April 26, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28443689/recent-achievements-in-tc-99m-radiopharmaceutical-direct-production-by-medical-cyclotrons
#9
Alessandra Boschi, Petra Martini, Micol Pasquali, Licia Uccelli
(99m)Tc is the most commonly used radionuclide in the field of diagnostic imaging, a noninvasive method intended to diagnose a disease, assess the disease state and monitor the effects of treatments. Annually, the use of (99m)Tc, covers about 85% of nuclear medicine applications. This isotope releases gamma rays at about the same wavelength as conventional X-ray diagnostic equipment, and owing to its short half-life (t½ = 6 hours) is ideal for diagnostic nuclear imaging. A patient can be injected with a small amount of (99m)Tc and within 24 hours almost 94% of the injected radionuclide would have decayed and left the body, limiting the patient's radiation exposure...
April 26, 2017: Drug Development and Industrial Pharmacy
https://www.readbyqxmd.com/read/28443502/fe3o4-sio2-kit-6-as-an-efficient-and-reusable-catalyst-for-the-synthesis-of-novel-derivatives-of-3-3-aryl-1-phenyl-1h-pyrazol-4-yl-methylene-bis-1h-indole
#10
Mohammad Nikpassand, Leila Zare Fekri, Mozhdeh Nabatzadeh
Korea advanced institute of science and technology cubic ordered mesoporous silica (KIT-6 mesoporous) silica coated magnetite nanoparticles, is an effective, eco-benign and recyclable catalyst for the electrophilic substitution reactions of indoles with various synthetized aldehydes to afford the corresponding novel diindolylmethanes in high yields and short reaction times. The catalyst can be recovered and reused without loss of activity. The work-up of the reaction consists of a simple separation, followed by concentration of the crude product and purification...
April 25, 2017: Combinatorial Chemistry & High Throughput Screening
https://www.readbyqxmd.com/read/28442430/design-and-purification-of-active-truncated-phosphoinositide-3-kinase-gamma-protein-constructs-for-structural-studies
#11
A Vujičić Žagar, L Scapozza, O Vadas
Phosphoinositide 3-kinase gamma (PI3Kγ) is a lipid kinase that plays a crucial role in cell migration, chemotaxis, oxidative burst and myocardial contractility. It is activated downstream of G protein-coupled receptors (GPCRs) and small GTPases of Ras superfamily. PI3Kγ is a heterodimer composed of a catalytic and a regulatory subunit that is expressed mostly in hematopoietic cells and in the heart. Although it has attracted a lot of attention because of its link with tumor inflammation and heart diseases, its regulation is still not fully understood...
April 22, 2017: Protein Expression and Purification
https://www.readbyqxmd.com/read/28442412/hsccc-separation-and-enantiomeric-distribution-of-key-volatile-constituents-of-piper-claussenianum-miq-c-dc-piperaceae
#12
André M Marques, Catharina E Fingolo, Maria Auxiliadora C Kaplan
High Speed Countercurrent Chromatography (HSCCC) technique was used for the preparative isolation of the major leishmanicidal compounds from the essential oils of Piper claussenianum species in Brazil. The essential oils from inflorescences of P. claussenianum were analyzed by GC-FID and GC-MS. The enantiomeric ratio of the major constituents of the P. claussenianum essential oils were determined using a Rt-DEXsm chiral capillary column by GC-FID analysis. It was found an enantiomeric excess of (+)-(E)-nerolidol in the leaves, and (+)-linalool and (+)-(E)-nerolidol in the inflorescences essential oil...
April 22, 2017: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/28441546/isolation-of-new-polyacetylenes-from-the-roots-of-eurycoma-longifolia-via-high-speed-counter-current-chromatography
#13
Minglong Wang, Huijuan Zou, Qibin Chen, Jianguo Cao, Haji Akber Aisa, Guozheng Huang
Eurycoma longifolia is a tropical plant of diverse applications in folk medicine, which occurs in Southeast Asia. In this study, pre-purified fraction (0.86g) of the crude extracts from the roots of E. longifolia, was subjected to preparative high-speed counter-current chromatography (HSCCC) with a two-phase solvent system composed of hexane-ethyl acetate-methanol-water (HEMWat) at a volume ratio of 5:2:5:2 (v/v). Longifolione A (1, 19mg, purity 96.0%) and longifolione C (3, 317mg, purity 96.2%), together with longifolione B (2, purity 77...
April 1, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28441470/al2o3-and-sio2-atomic-layer-deposition-layers-on-zno-photoanodes-and-degradation-mechanisms
#14
Qian Cheng, Manpuneet Kaur Benipal, Qianlang Liu, Xingye Wang, Peter A A Crozier, Candace K Chan, Robert J Nemanich
Strategies for protecting unstable semiconductors include the utilization of surface layers comprised of thin films deposited using atomic layer deposition (ALD). The protective layer is expected to: (1) be stable against reaction with photogenerated holes, (2) prevent direct contact of the unstable semiconductor with the electrolyte, and (3) prevent the migration of ions through the semiconductor/electrolyte interface, while still allowing photogenerated carriers to transport to the interface and participate in the desired redox reactions...
April 25, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28441012/rgb-switchable-porous-electrospun-nanofiber-chemoprobe-filter-prepared-from-multifunctional-copolymers-for-versatile-sensing-of-ph-and-heavy-metals
#15
Fang-Cheng Liang, Chi Ching Kuo, Bo-Yu Chen, Chia-Jung Cho, Chih-Chien Hung, Wen-Chang Chen, Redouane Borsali
Novel red-green-blue (RGB) switchable probes based on fluorescent porous electrospun (ES) nanofibers exhibiting high sensitivity to pH and mercury ions (Hg2+) were prepared with one type of copolymer (poly(methyl methacrylatete-co-1, 8-naphthalimide derivatives-co-rhodamine derivative); poly(MMA-co-BNPTU-co-RhBAM)) by using a single-capillary spinneret. The MMA, BNPTU, and RhBAM moieties were designed to (i) permit formation of porous fibers, (ii) fluoresce for Hg2+ detection, and (iii) fluoresce for pH, respectively...
April 25, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28440988/-how-safe-is-the-recombinant-human-growth-hormone
#16
Raúl Calzada-León
In this paper, several aspects related to the safety of the use of biosynthetic human growth hormone are reviewed. For example, its classification as a biosynthetic drug, the phases that need to be performed in Mexico to verify its safety (obtaining, purification, preclinical studies, clinical trials, and finally observational clinical studies), as well as the evidence that exists in relation to the association of intracranial hypertension, muscular events, scoliosis, slipped capital femoral epiphysis, obstructive sleep apnea, pancreatitis, alterations in cortisol, thyroid hormones alterations, cardiovascular disease, metabolic risk, mortality and cancer, adverse events not related to its use, and finally dosing and safety...
May 2017: Revista Médica del Instituto Mexicano del Seguro Social
https://www.readbyqxmd.com/read/28440667/camelus-dromedarius-glucose-transporter-4-in-silico-analysis-cloning-expression-purification-and-characterisation-in-e-coli
#17
Anwar Ahmed, Mohammed Arshad, Ajamaluddin Malik, Shama Parveen, Abdulrahman M Alsenaidy
Camels have exceptional carbohydrate metabolism as their plasma glucose level is high and have low whole body insulin sensitivity, similar to that observed in type 2 diabetes patients. We aimed at studing an important component of insulin signalling pathway, the GLUT4, in camel. Camelus dromedarius GLUT4 (CdGLUT4) CDS is 1530 nucleotide in length that encodes for a 55KDa protein. CdGLUT4 has 23 amino acid substitutions and 3N-glycosylation sites, compared to 2 in Human GLUT4. 3 D structures of CdGLUT4 and HsGLUT4 generated by homology modelling revealed conservation of characteristic signature motifs...
April 25, 2017: Archives of Physiology and Biochemistry
https://www.readbyqxmd.com/read/28440639/synthesis-of-chromones-from-1-1-diacylcyclopropanes-towards-the-synthesis-of-bromophycoic-acid-e
#18
Robert J Smith, Duong Nhu, Mitchell R Clark, Sinan Gai, Nigel T Lucas, Bill Corey Hawkins
A tandem deprotection-cyclization reaction of 1,1-diacylcyclopropanes is described which allows rapid access to structurally diverse 2,3-disubstituted chromones in good yields, and with straightforward purification. The utility of this reaction is showcased by the construction of the potent antibacterial marine natural product bromophycoic acid E scaffold.
April 25, 2017: Journal of Organic Chemistry
https://www.readbyqxmd.com/read/28440617/anchoring-tailored-low-index-faceted-biobr-nanoplates-onto-tio2-nanorods-to-enhance-the-stability-and-visible-light-driven-catalytic-activity
#19
Chao Xue, Tianxi Zhang, Shujiang Ding, Jinjia Wei, Guidong Yang
In this work, a fantastic one-dimensional (1D) BiOBr/TiO2 nanorods (NRs) heterojunction composite was rationally proposed and designed from the perspective of molecular and interface engineering. The fabricated intimately connected interfacial heterojunction between two-dimensional (2D) BiOBr nanoplates and 1D TiO2 NRs acts as the interfacial nano-channel to promote efficient interfacial charge migration and the separation of photogenerated electron-hole pairs. As a result, 1D BiOBr/TiO2 NRs heterojunctions exhibited outstanding visible-light photocatalytic activities and sustained cycling performance...
April 25, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28439867/mapping-protein-protein-interactions-using-affinity-purification-and-mass-spectrometry
#20
Chin-Mei Lee, Christopher Adamchek, Ann Feke, Dmitri A Nusinow, Joshua M Gendron
The mapping of protein-protein interaction (PPI) networks and their dynamics are crucial steps to deciphering the function of a protein and its role in cellular pathways, making it critical to have comprehensive knowledge of a protein's interactome. Advances in affinity purification and mass spectrometry technology (AP-MS) have provided a powerful and unbiased method to capture higher-order protein complexes and decipher dynamic PPIs. However, the unbiased calling of nonspecific interactions and the ability to detect transient interactions remains challenging when using AP-MS, thereby hampering the detection of biologically meaningful complexes...
2017: Methods in Molecular Biology
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