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https://www.readbyqxmd.com/read/29332142/purification-and-characterization-of-a-novel-organic-solvent-tolerant-and-cold-adapted-lipase-from-psychrobacter-sp-zy124
#1
Yue Zhang, Fangling Ji, Jingyun Wang, Zhongji Pu, Bo Jiang, Yongming Bao
By screening 25 different psychrophilic strains isolated from the Arctic habitat, we isolated a strain capable of producing lipase. We identified this strain as Psychrobacter sp. ZY124 based on the amplified 16S rDNA sequence. The lipase, named as Lipase ZC12, produced from the supernatant of Psychrobacter sp. ZY124 cultured at 15 °C was purified to homogeneity by ammonium sulfate precipitation followed by Phenyl Sepharose FF gel hydrophobic chromatography. Based on the obtained amino acid sequence, Lipase ZC12 is classified as a member of the Proteus/psychrophilic subfamily of lipase family I...
January 13, 2018: Extremophiles: Life Under Extreme Conditions
https://www.readbyqxmd.com/read/29331859/development-of-a-method-to-determine-axitinib-lapatinib-and-afatinib-in-plasma-by-micellar-liquid-chromatography-and-validation-by-the-european-medicines-agency-guidelines
#2
Jaume Albiol-Chiva, Josep Esteve-Romero, Juan Peris-Vicente
A method based on micellar liquid chromatography to quantify the tyrosine kinase inhibitors axitinib, lapatinib and afatinib in plasma is reported. The sample pretreatment was a simple 1/5-dilution in a pure micellar solution, filtration and direct injection, without requiring extraction or purification steps. The three drugs were resolved from the matrix in 17min, using an aqueous solution of 0.07M sodium dodecyl sulfate - 6.0% 1-pentanol, buffered at pH7 with 0.01M phosphate salt as mobile phase, running under isocratic mode at 1mL/min through a C18 column...
January 2, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29331827/pilot-scale-outdoor-photobioreactor-culture-of-the-marine-dinoflagellate-karlodinium-veneficum-production-of-a-karlotoxins-rich-extract
#3
L López-Rosales, A Sánchez-Mirón, F García-Camacho, A R Place, Yusuf Chisti, E Molina-Grima
A pilot-scale bioprocess was developed for the production of karlotoxin-enriched extracts of the marine algal dinoflagellate Karlodinium veneficum. A bubble column and a flat-panel photobioreactors (80-281 L) were used for comparative assessment of growth. Flow hydrodynamics and energy dissipation rates (EDR) in the bioreactors were characterized through robust computational fluid dynamic simulations. All cultures were conducted monoseptically outdoors. Bubble column (maximum cell productivity in semicontinuous operation of 58 × 103 cell mL-1 day-1) proved to be a better culture system for this alga...
December 30, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/29331784/degradation-of-dyes-by-peroxymonosulfate-activated-by-ternary-cofeni-layered-double-hydroxide-catalytic-performance-mechanism-and-kinetic-modeling
#4
Hanxuan Zeng, Weiqiu Zhang, Lin Deng, Jinming Luo, Shiqing Zhou, Xia Liu, Yong Pei, Zhou Shi, John Crittenden
Ternary CoFeNi-layered double hydroxide (CoFeNi-LDH) was synthesized and initially applied to activate peroxymonosulfate (PMS) for the degradation of Congo red (CR) and Rhodamine B (RhB). The results show that the CoFeNi-LDH/PMS system can efficiently degrade nearly 100% of 20 mg/L CR or 20 mg/L RhB within 6- and 10-min reaction times, respectively. And the catalyst exhibits higher degradation efficiency on CR than on RhB under identical conditions, which is confirmed by electron clouds of the highest occupied molecular orbital (HOMO) and lowest unoccupied molecular orbital (LUMO) performed by DFT calculations...
January 4, 2018: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/29331742/a-sensitive-and-selective-immunoaffinity-column-clean-up-coupled-to-uplc-ms-ms-for-determination-of-trace-methyl-3-quinoxaline-2-carboxylic-acid-in-animal-tissues
#5
Peipei Li, Xiaojun Zhang, Juanmei Zhang, Zhongyong Yan, Shuai Zhang, Si Chen, Yi Fang
This paper described a reliable and simple method for the selective determination of MQCA in animal tissues using ultra high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). A highly targeted immunoaffinity column was used for sample purification after enzymatic hydrolysis. The purified extracts were analyzed by reversed-phase HPLC-MS/MS in positive ESI and multiple reaction monitoring mode. The calibration curves showed good linearity with correlation coefficient (r2) larger than 0...
January 3, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29331223/structure-based-design-and-application-of-a-nucleotide-coenzyme-mimetic-ligand-application-to-the-affinity-purification-of-nucleotide-dependent-enzymes
#6
Marigianna Marinou, Dimitrios Platis, Farid S Ataya, Evangelia Chronopoulou, Dimitrios Vlachakis, Nikolaos E Labrou
In the present study, a structure-based approach was exploited for the in silico design of a nucleotide coenzyme mimetic ligand. The enzyme formate dehydrogenase (FDH) was employed as a model in our study. The biomimetic ligand was designed and synthesized based on a tryptamine/3-aminopropylphosphonic acid bi-substituted 1,3,5-triazine (Trz) scaffold (Tra-Trz-3APP), which potentially mimics the interactions of NAD+-FDH complex. Molecular docking studies of the biomimetic ligand predicted that it can occupy the same binding site as the natural coenzyme...
January 3, 2018: Journal of Chromatography. A
https://www.readbyqxmd.com/read/29331148/isolation-of-anti-extra-cellular-vesicle-single-domain-antibodies-by-direct-panning-on-vesicle-enriched-fractions
#7
Milica Popovic, Elisa Mazzega, Barbara Toffoletto, Ario de Marco
BACKGROUND: The thorough understanding of the physiological and pathological processes mediated by extracellular vesicles (EVs) is challenged by purification methods which are cumbersome, not reproducible, or insufficient to yield homogeneous material. Chromatography based on both ion-exchange and immune-capture can represent an effective method to improve EV purification and successive analysis. METHODS: Cell culture supernatant was used as a model sample for assessing the capacity of anion-exchange chromatography to separate distinct EV fractions and to isolate nanobodies by direct panning on whole EVs to recover binders specific for the native conformation of EV-surface epitopes and suitable to develop EV immune-capture reagents...
January 13, 2018: Microbial Cell Factories
https://www.readbyqxmd.com/read/29331014/biochemical-characterization-of-recombinant-thermostable-cohnella-sp-a01-%C3%AE-glucanase
#8
Meysam Rezaie, Saeed Aminzadeh, Farid Heidari, Masoud Mashhadi Akbar Boojar, Ali Asghar Karkhane
Background: Typically, non-cellulytic glucanase, including fungi and yeast cell wall hydrolyzing enzymes, are release by some symbiotic fungi and plants during the mycoparasitic fungi attack on plants. These enzymes are known as the defense mechanisms of plants. This study intends to investigate the biochemical properties of β-1,6-glucanase (bg16M) from native thermophilic bacteria, Cohnella A01. Methods: bg16M gene was cloned and expressed in E. coli BL21 (DE3)...
January 13, 2018: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/29330912/high-capacity-protein-a-affinity-chromatography-for-the-fast-quantification-of-antibodies-two-wavelength-detection-expands-linear-range
#9
Peter Satzer, Alois Jungbauer
The high-throughput analysis of antibodies from processes can be enhanced when the linear range is expanded and sample preparation is kept to a minimum. We developed a fast chromatography method based on a hexameric variant of Staphylococcal protein A immobilized on Toyopearl matrix, TSK 5 PW using two wavelengths. A protocol with 5 min runtime and a single-wavelength detection at 280 nm yielded an upper limit of quantification of 2.10 mg/mL and a lower limit of quantification of 0.06 mg/mL. The optimized method with a runtime of 2 min and two-wavelength detection at 280 and 300 nm allowed us to span a valid concentration range of 0...
January 13, 2018: Journal of Separation Science
https://www.readbyqxmd.com/read/29330328/assembling-a-cellular-user-manual-for-the-brain
#10
Steven A Sloan, Ben A Barres
For many years, efforts to decipher the various cellular components that comprise the central nervous system were stymied by a lack of technical strategies for isolating and profiling the brain's resident cell types. The advent of transcriptional profiling, combined with powerful new purification schemes, changed this reality and transformed our understanding of the macroglial populations within the brain. Here, we chronicle the historical context and scientific setting for our efforts to transcriptionally profile neurons, astrocytes, and oligodendrocytes, and highlight some of the profound discoveries that were cultivated by these data...
January 12, 2018: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
https://www.readbyqxmd.com/read/29329805/preparative-expression-and-purification-of-a-nacreous-protein-n16-and-testing-its-effect-on-osteoporosis-rat-model
#11
Zhen-Yan Xu, Yu-Ling Liu, Jia-Bi Lin, Ke-Ling Cheng, Yong-Gang Wang, Hong-Liang Yao, Wei-Peng, Hoi-Yan Wu, Wei-Wei Su, Pang-Chui Shaw, Pei-Bo Li
N16, a nacreous protein isolated from Pinctada martensii, is related to nacreous layer formation. Our previous study indicated that N16 showed dual regulatory effects by inducing osteoblast biomineralization as well as inhibiting osteoclast formation. In order to obtain large quantity of N16 for animal experiment and clinical trial, a fermentation and preparative purification method was established. The N16 cDNA was cloned to a BL21(DE3)plysE-pET32a vector and grown in a 20 L fermenter. The medium, temperature, pH and dissolved oxygen (DO) were optimized...
January 9, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29329804/a-rapid-and-sensitive-fluorometric-method-for-determination-of-aldehyde-oxidase-activity
#12
Nancy Apenova, Hui Peng, Markus Hecker, Markus Brinkmann
Previous research has characterized the important role of aldehyde oxidases (AOX) in biotransformation of N-heterocyclic therapeutic drugs and environmental contaminants in mammals. Research pertaining to AOX activity in non-mammalian vertebrates, however, is scarce, despite its biological role as a potentially important metabolic pathway for xenobiotics. One of the limiting factors of research on AOX is that available photometric methods are relatively insensitive, limited in throughput, and prone to cross-reactivity from other enzymes...
January 9, 2018: Toxicology and Applied Pharmacology
https://www.readbyqxmd.com/read/29329014/nanoadsorbents-based-on-conducting-polymer-nanocomposites-with-main-focus-on-polyaniline-and-its-derivatives-for-removal-of-heavy-metal-ions-dyes-a-review
#13
REVIEW
Ehsan Nazarzadeh Zare, Ahmad Motahari, Mika Sillanpää
Water contamination by toxic heavy metal ions and dyes remains a serious public health problem for humans, so attention on specific methods and technologies to remove heavy metal ions and dyes from wastewaters/aqueous solutions are desired. Numerous adsorbents have been reported for the removal of heavy metal ions/dyes from wastewaters/aqueous solutions. Polyaniline (PANI) and its derivatives, as conducting polymers, are good adsorbents to remove various kinds of heavy metal ions and dyes from wastewaters/aqueous solutions...
January 9, 2018: Environmental Research
https://www.readbyqxmd.com/read/29327488/isolation-of-human-photoreceptor-precursors-via-a-cell-surface-marker-panel-from-stem-cell-derived-retinal-organoids-and-fetal-retinae
#14
Jörn Lakowski, Emily Welby, Dimitri Budinger, Fabiana Di Marco, Valentina Di Foggia, James W B Bainbridge, Kyle Wallace, David M Gamm, Robin R Ali, Jane C Sowden
Loss of photoreceptor cells due to retinal degeneration is one of the main causes of blindness in the developed world. Although there is currently no effective treatment, cell replacement therapy using stem-cell-derived photoreceptor cells may be a feasible future treatment option. In order to ensure safety and efficacy of this approach, robust cell isolation and purification protocols must be developed. To this end, we previously developed a biomarker panel for the isolation of mouse photoreceptor precursors from the developing mouse retina and mouse embryonic stem cell cultures...
January 12, 2018: Stem Cells
https://www.readbyqxmd.com/read/29327466/analysis-of-trace-levels-of-impurities-and-hydrogen-isotopes-in-helium-purge-gas-using-gas-chromatography-for-tritium-extraction-system-of-an-indian-lead-lithium-ceramic-breeder-test-blanket-module
#15
V Gayathri Devi, Amit Sircar, Deepak Yadav, Jayraj Parmar
In the fusion fuel cycle, the accurate analysis and understanding of the chemical composition of any gas mixture is of great importance for the efficient design of a tritium extraction and purification system or any tritium handling system. Methods like laser Raman spectroscopy and gas chromatography with thermal conductivity detector have been considered for hydrogen isotopes analyses in fuel cycles. Gas chromatography with a cryogenic separation column has been used for the analysis of hydrogen isotopes gas mixtures in general due to its high reliability and ease of operation...
January 12, 2018: Journal of Separation Science
https://www.readbyqxmd.com/read/29326991/a-facile-one-pot-synthesis-of-acrylated-hyaluronic-acid
#16
Sigen A, Qian Xu, Peter McMichael, Yongsheng Gao, Xiaolin Li, Xi Wang, Udo Greiser, Dezhong Zhou, Wenxin Wang
The synthesis of acrylated hyaluronic acid (HA-A) normally requires 2 to 3 steps of modification, needs laborious purification and also increases the risks of HA degradation. Here, we report that the conjugation of acrylate groups to hyaluronic acid can be successfully achieved via a new facile one-pot approach. Two types of new HA-A hydrogels (via chemical or UV crosslinking) were developed and applied for 3D cell encapsulation.
January 12, 2018: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/29326975/identifying-the-substrate-proteins-of-u-box-e3s-e4b-and-chip-by-orthogonal-ubiquitin-transfer
#17
Karan Bhuripanyo, Yiyang Wang, Xianpeng Liu, Li Zhou, Ruochuan Liu, Duc Duong, Bo Zhao, Yingtao Bi, Han Zhou, Geng Chen, Nicholas T Seyfried, Walter J Chazin, Hiroaki Kiyokawa, Jun Yin
E3 ubiquitin (UB) ligases E4B and carboxyl terminus of Hsc70-interacting protein (CHIP) use a common U-box motif to transfer UB from E1 and E2 enzymes to their substrate proteins and regulate diverse cellular processes. To profile their ubiquitination targets in the cell, we used phage display to engineer E2-E4B and E2-CHIP pairs that were free of cross-reactivity with the native UB transfer cascades. We then used the engineered E2-E3 pairs to construct "orthogonal UB transfer (OUT)" cascades so that a mutant UB (xUB) could be exclusively used by the engineered E4B or CHIP to label their substrate proteins...
January 2018: Science Advances
https://www.readbyqxmd.com/read/29326063/efficient-purification-of-a-highly-active-h-subunit-of-tyrosinase-from-agaricus-bisporus
#18
David Lopez-Tejedor, Jose M Palomo
A highly-active tyrosinase (H subunit) isoform has been purified from a commercial crude extract of Agaricus bisporus by a specific, two step-hydrophobic chromatography cascade process based on the differential adsorption of the proteins from the extract to hydrophobic-functionalized supports. At first, commercial, crude tyrosinase from Agaricus bisporus (AbTyr) dissolved in aqueous media was added to octadecyl-Sepabeads matrix at 25 °C. Under these conditions, the support specifically adsorbed a protein with a molecular weight of 47 kDa which showed no tyrosinase activity...
January 8, 2018: Protein Expression and Purification
https://www.readbyqxmd.com/read/29325877/dmcatd-a-cathepsin-d-like-peptidase-of-the-hematophagous-insect-dipetalogaster-maxima-hemiptera-reduviidae-purification-bioinformatic-analyses-and-the-significance-of-its-interaction-with-lipophorin-in-the-internalization-by-developing-oocytes
#19
Jimena Leyria, Leonardo L Fruttero, Rodrigo Ligabue-Braun, Marina S Defferrari, Estela L Arrese, José L Soulages, Beatriz P Settembrini, Celia R Carlini, Lilián E Canavoso
DmCatD, a cathepsin D-like peptidase of the hematophagous insect Dipetalogaster maxima, is synthesized by the fat body and the ovary and functions as yolk protein precursor. Functionally, DmCatD is involved in vitellin proteolysis. In this work, we purified and sequenced DmCatD, performed bioinformatic analyses and investigated the events involved in its targeting and storage in developing oocytes. By ion exchange and gel filtration chromatography, DmCatD was purified from egg homogenates and its identity was confirmed by mass spectrometry...
January 8, 2018: Journal of Insect Physiology
https://www.readbyqxmd.com/read/29323681/the-structure-of-a-lanthanide-complex-at-an-extractant-water-interface-studied-using-heterodyne-detected-vibrational-sum-frequency-generation
#20
Ryoji Kusaka, Masayuki Watanabe
Solvent extraction plays an integral part in the separation and purification of metals. Because extractants generally used as complexing agents for metal extractions, such as di-(2-ethylhexyl)phosphoric acid (HDEHP) for lanthanide extractions, are amphiphilic, they come to an organic/water interface, and the interface plays a crucial role as the site of the formation of metal complexes and the subsequent transfer reaction to an organic phase. Despite the importance of the interface for metal solvent extractions, its molecular-level structure is unclear because of the experimental difficulties...
January 11, 2018: Physical Chemistry Chemical Physics: PCCP
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