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https://www.readbyqxmd.com/read/27936100/a-high-resolution-crystal-structure-of-a-psychrohalophilic-%C3%AE-carbonic-anhydrase-from-photobacterium-profundum-reveals-a-unique-dimer-interface
#1
Vijayakumar Somalinga, Greg Buhrman, Ashikha Arun, Robert B Rose, Amy M Grunden
Bacterial α-carbonic anhydrases (α-CA) are zinc containing metalloenzymes that catalyze the rapid interconversion of CO2 to bicarbonate and a proton. We report the first crystal structure of a pyschrohalophilic α-CA from a deep-sea bacterium, Photobacterium profundum. Size exclusion chromatography of the purified P. profundum α-CA (PprCA) reveals that the protein is a heterogeneous mix of monomers and dimers. Furthermore, an "in-gel" carbonic anhydrase activity assay, also known as protonography, revealed two distinct bands corresponding to monomeric and dimeric forms of PprCA that are catalytically active...
2016: PloS One
https://www.readbyqxmd.com/read/27936069/multimodal-imaging-of-photoreceptor-structure-in-choroideremia
#2
Lynn W Sun, Ryan D Johnson, Vesper Williams, Phyllis Summerfelt, Alfredo Dubra, David V Weinberg, Kimberly E Stepien, Gerald A Fishman, Joseph Carroll
PURPOSE: Choroideremia is a progressive X-linked recessive dystrophy, characterized by degeneration of the retinal pigment epithelium (RPE), choroid, choriocapillaris, and photoreceptors. We examined photoreceptor structure in a series of subjects with choroideremia with particular attention to areas bordering atrophic lesions. METHODS: Twelve males with clinically-diagnosed choroideremia and confirmed hemizygous mutations in the CHM gene were examined. High-resolution images of the retina were obtained using spectral domain optical coherence tomography (SD-OCT) and both confocal and non-confocal split-detector adaptive optics scanning light ophthalmoscope (AOSLO) techniques...
2016: PloS One
https://www.readbyqxmd.com/read/27935976/flexible-and-stretchable-microneedle-patches-with-integrated-rigid-stainless-steel-microneedles-for-transdermal-biointerfacing
#3
Mina Rajabi, Niclas Roxhed, Reza Zandi Shafagh, Tommy Haraldson, Andreas Christin Fischer, Wouter van der Wijngaart, Göran Stemme, Frank Niklaus
This paper demonstrates flexible and stretchable microneedle patches that combine soft and flexible base substrates with hard and sharp stainless steel microneedles. An elastomeric polymer base enables conformal contact between the microneedle patch and the complex topography and texture of the underlying skin, while robust and sharp stainless steel microneedles reliably pierce the outer layers of the skin. The flexible microneedle patches have been realized by magnetically assembling short stainless steel microneedles into a flexible polymer supporting base...
2016: PloS One
https://www.readbyqxmd.com/read/27935165/long-term-forest-resilience-to-climate-change-indicated-by-mortality-regeneration-and-growth-in-semi-arid-southern-siberia
#4
Chongyang Xu, Hongyan Liu, Oleg A Anenkhonov, Andrey Yu Korolyuk, Denis V Sandanov, Larisa D Balsanova, Bulat B Naidanov, Xiuchen Wu
Several studies have documented that regional climate warming and the resulting increase in drought stress have triggered increased tree mortality in semi-arid forests with unavoidable impacts on regional and global carbon sequestration. Although climate warming is projected to continue into the future, studies examining long-term resilience of semi-arid forests against climate change are limited. In this study, long-term forest resilience was defined as the capacity of forest recruitment to compensate for losses from mortality...
December 9, 2016: Global Change Biology
https://www.readbyqxmd.com/read/27935149/the-sorting-receptor-sorcs3-is-a-stronger-regulator-of-glutamate-receptor-functions-compared-to-gabaergic-mechanisms-in-the-hippocampus
#5
Gitte B Christiansen, Katrine Andersen, Sarah Riis, Anders Nykjaer, Ulrik Bølcho, Morten Skovgaard Jensen, Mai Marie Holm
Correct function of glutamate receptors in the postsynaptic density is crucial to synaptic function and plasticity. SorCS3 (sortilin-related receptor CNS expressed 3) is a sorting receptor which previously has been shown to interact with the key postsynaptic proteins; PSD-95 and PICK1. In this study, we employed electrophysiological analyses of acute brain slices combined with immunohistochemistry to define the role of SorCS3 in hippocampal synapses in CA1 and the dentate gyrus. We analyzed a juvenile (P17-21) and a young adult (P55-65) group of animals from a Sorcs3 knockout mouse model...
December 9, 2016: Hippocampus
https://www.readbyqxmd.com/read/27934520/two-step-membrane-binding-of-ndpk-b-induces-membrane-fluidity-decrease-and-changes-in-lipid-lateral-organization-and-protein-cluster-formation
#6
Liberty Francois-Moutal, Myriam M Ouberai, Ofelia Maniti, Mark E Welland, Agnieszka Strzelecka-Kiliszek, Marcin Wos, Slawomir Pikula, Joanna Bandorowicz-Pikula, Olivier Marcillat, Thierry Granjon
Nucleoside diphosphate kinases (NDPKs) are crucial elements in a wide array of cellular physiological or pathophysiological processes such as apoptosis, proliferation, or metastasis formation. Among the NDPK isoenzymes, NDPK-B, a cytoplasmic protein, was reported to be associated with several biological membranes such as plasma or endoplasmic reticulum membranes. Using several membrane models (liposomes, lipid monolayers, and supported lipid bilayers) associated with biophysical approaches, we show that lipid membrane binding occurs in a two-step process: first, initiation by a strong electrostatic adsorption process and followed by shallow penetration of the protein within the membrane...
December 6, 2016: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/27933631/hemicrania-continua-like-headache-related-to-transdermal-nitroglycerine-therapy
#7
Federico Mainardi, Giorgio Zanchin, Ferdinando Maggioni
Several cases of symptomatic hemicrania continua (HC) have been reported. A 66-year-old man, suffering from migraine without aura, presented with a four month history of a new headache fulfilling the ICHD 3beta clinical criteria for HC. HC onset was strictly related to the use of transdermal nitroglycerine patch (TNP). In agreement with the cardiologist, TNP was discontinued and the headache promptly disappeared; symptoms reappeared within 6-12 hours after nitroglycerine reintroduction. After permanent discontinuation of TNP, headache disappeared at one year follow-up...
December 9, 2016: Headache
https://www.readbyqxmd.com/read/27933319/a-metabolic-widget-adjusts-the-phosphoenolpyruvate-dependent-fructose-influx-in-pseudomonas-putida
#8
Max Chavarría, Ángel Goñi-Moreno, Víctor de Lorenzo, Pablo I Nikel
Fructose uptake in the soil bacterium Pseudomonas putida occurs through a canonical phosphoenolpyruvate (PEP)-dependent sugar transport system (PTS(Fru)). The logic of the genetic circuit that rules its functioning is puzzling: the transcription of the fruBKA operon, encoding all the components of PTS(Fru), can escape the repression exerted by the catabolite repressor/activator protein Cra solely in the presence of intracellular fructose-1-P, an agonist formed only when fructose has been already transported...
November 2016: MSystems
https://www.readbyqxmd.com/read/27933048/clay-components-in-soil-dictate-environmental-stability-and-bioavailability-of-cervid-prions-in-mice
#9
A Christy Wyckoff, Sarah Kane, Krista Lockwood, Jeff Seligman, Brady Michel, Dana Hill, Aimee Ortega, Mihnea R Mangalea, Glenn C Telling, Michael W Miller, Kurt Vercauteren, Mark D Zabel
Chronic wasting disease (CWD) affects cervids and is the only known prion disease to affect free-ranging wildlife populations. CWD spread continues unabated, and exact mechanisms of its seemingly facile spread among deer and elk across landscapes in North America remain elusive. Here we confirm that naturally contaminated soil contains infectious CWD prions that can be transmitted to susceptible model organisms. We show that smectite clay content of soil potentiates prion binding capacity of different soil types from CWD endemic and non-endemic areas, likely contributing to environmental stability of bound prions...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27932918/salix-transect-of-europe-variation-in-ploidy-and-genome-size-in-willow-associated-common-nettle-urtica-dioica-l-sens-lat-from-greece-to-arctic-norway
#10
Quentin Cronk, Oriane Hidalgo, Jaume Pellicer, Diana Percy, Ilia J Leitch
BACKGROUND: The common stinging nettle, Urtica dioica L. sensu lato, is an invertebrate "superhost", its clonal patches maintaining large populations of insects and molluscs. It is extremely widespread in Europe and highly variable, and two ploidy levels (diploid and tetraploid) are known. However, geographical patterns in cytotype variation require further study. NEW INFORMATION: We assembled a collection of nettles in conjunction with a transect of Europe from the Aegean to Arctic Norway (primarily conducted to examine the diversity of Salix and Salix-associated insects)...
2016: Biodiversity Data Journal
https://www.readbyqxmd.com/read/27932454/inducible-colonic-m-cells-are-dependent-on-tnfr2-but-not-lt%C3%AE-r-identifying-distinct-signaling-requirements-for-constitutive-versus-inducible-m-cells
#11
Erinn A Parnell, Erin M Walch, David D Lo
BACKGROUND AND AIMS: M cells associated with organized lymphoid tissues such as intestinal Peyer's patches provide surveillance of the intestinal lumen. Inflammation or infection in the colon can induce an M cell population associated with lymphoid infiltrates; paradoxically, induction is dependent on the inflammatory cytokine Tumor Necrosis Factor (TNF)-α. Anti-TNFα blockade is an important therapeutic in Inflammatory Bowel Disease, so understanding the effects of TNFα signaling is important in refining therapeutics...
December 7, 2016: Journal of Crohn's & Colitis
https://www.readbyqxmd.com/read/27932442/contraceptive-sales-in-the-setting-of-the-zika-virus-epidemic
#12
Luis Bahamondes, Moazzam Ali, Ilza Monteiro, Arlete Fernandes
STUDY QUESTION: Has there been any influence of the Zika virus (ZIKV) outbreak on the sales of contraceptive methods in Brazil? SUMMARY ANSWER: Contraceptive sales in the 24 months of evaluation showed little variation and no significant change has been observed since the ZIKV outbreak. WHAT IS KNOWN ALREADY: Transmission of ZIKV is primarily by Aedes aegypti mosquitoes; however, sexual transmission has also been described. The association of several birth defects and the ZIKV infection during pregnancy has been established, and it was estimated in Bahia, Brazil that the infection rate could range from 10% to 80%...
December 7, 2016: Human Reproduction
https://www.readbyqxmd.com/read/27932132/collateral-development-in-thrombosis-of-the-hepatic-artery-after-transplantation
#13
C M García Bernardo, B Argüelles García, P Redondo Buil, A Miyar de León, L González Dieguez, V Cadahía Rodrigo, I González-Pinto Arrillaga, L Vázquez Velasco
OBJECTIVES: The authors sought to identify strictures or hepatic artery obstruction with posterior collateral transformation in our series of liver transplantation, treatment, and evolution. The thrombosis or severe hepatic artery stenosis sometimes presents a compensation mechanism, the collateral transformation of the artery. MATERIAL AND METHODS: From April 2002 to December 2011 we collected 18 cases of collateral transformation. We analyzed data regarding the transplantation, diagnosis, treatment, clinical evolution, liver function, and Doppler-ultrasound...
November 2016: Transplantation Proceedings
https://www.readbyqxmd.com/read/27931745/histone-acetylation-regulates-chromatin-accessibility-role-of-h4k16-in-inter-nucleosome-interaction
#14
Ruihan Zhang, Jochen Erler, Jörg Langowski
The N-terminal tail of histone H4 is an indispensable mediator for inter-nucleosome interaction, which is required for chromatin fiber condensation. H4K16 acetylation (H4K16Ac) activates gene transcription by influencing both chromatin structure and interplay with nonhistone proteins. To understand the influence of H4K16Ac on inter-nucleosome interaction, we performed a simulation study for the H4 tail in the context of two nucleosomes in neighboring unit cells in the crystal structure. The binding conformation of H4 tail with/without K16Ac was sampled by replica exchange with solute tempering, and the free energy landscape was explored by metadynamics...
December 6, 2016: Biophysical Journal
https://www.readbyqxmd.com/read/27930958/protected-areas-and-agricultural-expansion-biodiversity-conservation-versus-economic-growth-in-the-southeast-of-brazil
#15
Mayra Cristina Prado de Moraes, Kaline de Mello, Rogério Hartung Toppa
The conversion of natural ecosystems to agricultural land and urban areas plays a threat to the protected areas and the natural ecosystems conservation. The aim of this paper is to provide an analysis of the agricultural expansion and its impact on the landscape spatial and temporal patterns in a buffer zone of a protected area located in the transition zone between the Atlantic Forest and Cerrado, in the State of São Paulo, Brazil. The land use and land cover were mapped between 1971 and 2008 and landscape metrics were calculated to provide a spatiotemporal analysis of the forest structure and the expansion of the croplands...
December 5, 2016: Journal of Environmental Management
https://www.readbyqxmd.com/read/27930519/who-are-the-users-of-a-traditional-chinese-sanfu-acupoint-herbal-patching-therapy-in-china-a-cross-sectional-survey
#16
Fen Zhou, Hong-Jun Wu, Jun-Ping Zhai, Gui-Yan Zhang, Yan Shao, Xia Tian, Ru-Yu Xia, Li-Yan Jia, Wen-Yuan Li, Ning Liang, Xiao-Yi Yan, Ruo-Han Wu, Kang Zhang, Xin-Lin Li, Cong-Cong Wang, Jian-Ping Liu
Sanfu acupoint herbal patching (SAHP) is a unique traditional Chinese medicine therapy, which has become popular for preventing acute attack of respiratory diseases such as asthma and chronic obstructive pulmonary disease, in many regions of mainland China. However, the knowledge about its users is lacking, especially the characteristics of the users and their experience and perspectives.To investigate the demographics of users, conditions for its use and the previous experience of SAHP, as well as users' perspectives to provide baseline information for its practice...
December 2016: Medicine (Baltimore)
https://www.readbyqxmd.com/read/27930311/spop-promotes-skeletal-development-and-homeostasis-by-positively-regulating-ihh-signaling
#17
Hongchen Cai, Aimin Liu
Indian Hedgehog (Ihh) regulates chondrocyte and osteoblast differentiation through the Glioma-associated oncogene homolog (Gli) transcription factors. Previous in vitro studies suggested that Speckle-type POZ protein (Spop), part of the Cullin-3 (Cul3) ubiquitin ligase complex, targets Gli2 and Gli3 for degradation and negatively regulates Hedgehog (Hh) signaling. In this study, we found defects in chondrocyte and osteoblast differentiation in Spop-null mutant mice. Strikingly, both the full-length and repressor forms of Gli3, but not Gli2, were up-regulated in Spop mutants, and Ihh target genes Patched 1 (Ptch1) and parathyroid hormone-like peptide (Pthlh) were down-regulated, indicating compromised Hh signaling...
December 5, 2016: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/27929727/television-video-games-in-the-treatment-of-amblyopia-in-children-aged-4-7-years
#18
Subhash Dadeya, Sonal Dangda
AIM: To investigate the role of television video games in childhood amblyopia treatment. METHOD: This prospective, randomized, interventional study included 40 patients between 4-7 years of age, with unilateral amblyopia (visual acuity in amblyopic eye between 1-0.6 LogMAR equivalents) attending the squint clinic at a tertiary eye hospital. All patients were prescribed optimal spectacle correction and occlusion therapy, i.e. full time patching according to patient's age, was initiated after six weeks...
December 2016: Strabismus
https://www.readbyqxmd.com/read/27929726/treatment-of-amblyopia-using-personalized-dosing-strategies-statistical-modelling-and-clinical-implementation
#19
Michael P Wallace, Catherine E Stewart, Merrick J Moseley, David A Stephens, Alistair R Fielder
PURPOSE: To generate a statistical model for personalizing a patient's occlusion therapy regimen. METHODS: Statistical modelling was undertaken on a combined data set of the Monitored Occlusion Treatment of Amblyopia Study (MOTAS) and the Randomized Occlusion Treatment of Amblyopia Study (ROTAS). This exercise permits the calculation of future patients' total effective dose (TED)-that predicted to achieve their best attainable visual acuity. Daily patching regimens (hours/day) can be calculated from the TED...
December 2016: Strabismus
https://www.readbyqxmd.com/read/27929520/the-minimum-area-requirements-mar-for-giant-panda-an-empirical-study
#20
Jing Qing, Zhisong Yang, Ke He, Zejun Zhang, Xiaodong Gu, Xuyu Yang, Wen Zhang, Biao Yang, Dunwu Qi, Qiang Dai
Habitat fragmentation can reduce population viability, especially for area-sensitive species. The Minimum Area Requirements (MAR) of a population is the area required for the population's long-term persistence. In this study, the response of occupancy probability of giant pandas against habitat patch size was studied in five of the six mountain ranges inhabited by giant panda, which cover over 78% of the global distribution of giant panda habitat. The probability of giant panda occurrence was positively associated with habitat patch area, and the observed increase in occupancy probability with patch size was higher than that due to passive sampling alone...
December 8, 2016: Scientific Reports
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