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JOURNAL ARTICLE
Yeonju Bae, Ajung Kim, Chang-Hoon Cho, Donggyu Kim, Hyun-Gug Jung, Seong-Seop Kim, Jiyun Yoo, Jae-Yong Park, Eun Mi Hwang
Volume-regulated anion channels (VRACs) are involved in cellular functions such as regulation of cell volume, proliferation, migration, and cell death. Although leucine-rich repeat-containing 8A (LRRC8A) has been characterized as a molecular component of VRACs, here we show that Drosophila melanogaster tweety homologue 1 and 2 (TTYH1 and TTYH2) are critical for VRAC currents in cancer cells. LRRC8A-independent VRAC currents were present in the gastric cancer cell line SNU-601, but almost completely absent in its cisplatin-resistant derivative SNU-601-R10 (R10)...
June 9, 2019: Cells
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JOURNAL ARTICLE
Young-Eun Han, Jea Kwon, Joungha Won, Heeyoung An, Minwoo Wendy Jang, Junsung Woo, Je Sun Lee, Min Gu Park, Bo-Eun Yoon, Seung Eun Lee, Eun Mi Hwang, Jae-Young Jung, Hyungju Park, Soo-Jin Oh, C Justin Lee
In the brain, a reduction in extracellular osmolality causes water-influx and swelling, which subsequently triggers Cl- - and osmolytes-efflux via volume-regulated anion channel (VRAC). Although LRRC8 family has been recently proposed as the pore-forming VRAC which is activated by low cytoplasmic ionic strength but not by swelling, the molecular identity of the pore-forming swelling-dependent VRAC (VRACswell ) remains unclear. Here we identify and characterize Tweety-homologs (TTYH1, TTYH2, TTYH3) as the major VRACswell in astrocytes...
April 2019: Experimental Neurobiology
#23
EDITORIAL
Yasunobu Okada
No abstract text is available yet for this article.
April 2019: Experimental Neurobiology
#24
JOURNAL ARTICLE
Hai-Ning Wu, Xiu-Li Cao, Zheng Fang, Yu-Fei Zhang, Wen-Juan Han, Kang-Yi Yue, Yuan Cao, Min-Hua Zheng, Li-Li Wang, Hua Han
Mammalian neural stem cells (NSCs) are not only responsible for normal development of the central nervous system (CNS), but also participate in brain homeostasis and repair, thus hold promising clinical potentials in the treatment of neurodegenerative diseases and trauma. However the molecular networks regulating the stemness and differentiation of NSCs have not been fully understood. In this study, we show that Tweety-homolog 1 (Ttyh1), a five-pass transmembrane protein specifically expressed in mouse brain, is involved in maintaining stemness of murine NSCs...
June 30, 2019: Biochemical and Biophysical Research Communications
#25
JOURNAL ARTICLE
Rhonda C Boyd, Jordan Price, Marjie Mogul, Tweety Yates, James P Guevara
OBJECTIVE: This pilot study describes the adaptation of a parenting group intervention for social media, and examines the feasibility, acceptability and initial outcomes of the adapted intervention for mothers with postpartum depression symptoms. BACKGROUND: Postpartum depression can negatively affect parenting and the parent-infant relationship. Mothers with postpartum depression symptoms experience barriers to access in-person parenting interventions. METHODS: A small, randomised controlled trial was conducted with an adapted parenting intervention delivered via social media (Facebook) or in-person for mothers who screened positive for depression in paediatric clinics...
December 17, 2018: Journal of Reproductive and Infant Psychology
#26
JOURNAL ARTICLE
Juwan Kim, Dasol Han, Sung-Hyun Byun, Mookwang Kwon, Jae Youl Cho, Samuel J Pleasure, Keejung Yoon
Despite growing evidence linking Drosophila melanogaster tweety-homologue 1 (Ttyh1) to normal mammalian brain development and cell proliferation, its exact role has not yet been determined. Here, we show that Ttyh1 is required for the maintenance of neural stem cell (NSC) properties as assessed by neurosphere formation and in vivo analyses of cell localization after in utero electroporation. We find that enhanced Ttyh1-dependent stemness of NSCs is caused by enhanced γ-secretase activity resulting in increased levels of Notch intracellular domain (NICD) production and activation of Notch targets...
November 2018: EMBO Reports
#27
JOURNAL ARTICLE
Erik Jung, Matthias Osswald, Jonas Blaes, Benedikt Wiestler, Felix Sahm, Torsten Schmenger, Gergely Solecki, Katrin Deumelandt, Felix T Kurz, Ruifan Xie, Sophie Weil, Oliver Heil, Carina Thomé, Miriam Gömmel, Mustafa Syed, Peter Häring, Peter E Huber, Sabine Heiland, Michael Platten, Andreas von Deimling, Wolfgang Wick, Frank Winkler
Early and progressive colonization of the healthy brain is one hallmark of diffuse gliomas, including glioblastomas. We recently discovered ultralong (>10 to hundreds of microns) membrane protrusions [tumor microtubes (TMs)] extended by glioma cells. TMs have been associated with the capacity of glioma cells to effectively invade the brain and proliferate. Moreover, TMs are also used by some tumor cells to interconnect to one large, resistant multicellular network. Here, we performed a correlative gene-expression microarray and in vivo imaging analysis, and identified novel molecular candidates for TM formation and function...
July 19, 2017: Journal of Neuroscience
#28
JOURNAL ARTICLE
Laura B Silverman, Inge-Marie Eigsti, Loisa Bennetto
This study examined whether individuals with autism spectrum disorder (ASD) produce co-speech gestures similarly to typically developing (TD) peers. Participants were 20 youth ages 10-18 years with high-functioning ASD and 21 TD controls matched on age, gender, verbal IQ, and handedness. Gestures were elicited using a classic narrative-retelling task, in which participants watched a Tweety and Sylvester cartoon and retold the cartoon to a confederate. Analyses compared gesture rate, type, and viewpoint (character, observer, dual) across groups...
August 2017: Autism Research: Official Journal of the International Society for Autism Research
#29
JOURNAL ARTICLE
Rebekah M Dedrick, Deborah Jacobs-Sera, Carlos A Guerrero Bustamante, Rebecca A Garlena, Travis N Mavrich, Welkin H Pope, Juan C Cervantes Reyes, Daniel A Russell, Tamarah Adair, Richard Alvey, J Alfred Bonilla, Jerald S Bricker, Bryony R Brown, Deanna Byrnes, Steven G Cresawn, William B Davis, Leon A Dickson, Nicholas P Edgington, Ann M Findley, Urszula Golebiewska, Julianne H Grose, Cory F Hayes, Lee E Hughes, Keith W Hutchison, Sharon Isern, Allison A Johnson, Margaret A Kenna, Karen K Klyczek, Catherine M Mageeney, Scott F Michael, Sally D Molloy, Matthew T Montgomery, James Neitzel, Shallee T Page, Marie C Pizzorno, Marianne K Poxleitner, Claire A Rinehart, Courtney J Robinson, Michael R Rubin, Joseph N Teyim, Edwin Vazquez, Vassie C Ware, Jacqueline Washington, Graham F Hatfull
Temperate phages are common, and prophages are abundant residents of sequenced bacterial genomes. Mycobacteriophages are viruses that infect mycobacterial hosts including Mycobacterium tuberculosis and Mycobacterium smegmatis, encompass substantial genetic diversity and are commonly temperate. Characterization of ten Cluster N temperate mycobacteriophages revealed at least five distinct prophage-expressed viral defence systems that interfere with the infection of lytic and temperate phages that are either closely related (homotypic defence) or unrelated (heterotypic defence) to the prophage...
January 9, 2017: Nature Microbiology
#30
JOURNAL ARTICLE
Xiu-Li Cao, Xing Zhang, Yu-Fei Zhang, Yi-Zhe Zhang, Chang-Geng Song, Fan Liu, Yi-Yang Hu, Min-Hua Zheng, Hua Han
Ttyh1 is a murine homolog of the Drosophila Tweety and is predicted as a five-pass transmembrane protein. The Ttyh1 mRNA is expressed in mouse brain tissues with a restricted pattern and in human glioma cells. Ttyh1 protein may function as a large-conductance chloride channel, however, the role of Ttyh1 in normal neural development and tumorigenesis has been largely unknown, at least partially due to the lack of effective antibodies. Here we report the expression in E. coli and purification of two recombinant Ttyh1 protein fragments corresponding to one of the predicted extracellular domains and the carboxyl terminus of the mouse Ttyh1...
February 2017: Protein Expression and Purification
#31
JOURNAL ARTICLE
Characterization of tweety gene (ttyh1-3) expression in Xenopus laevis during embryonic development.
Andrew D Halleran, Morgan Sehdev, Brian A Rabe, Ryan W Huyck, Cheyenne C Williams, Margaret S Saha
The tweety family of genes encodes large-conductance chloride channels and has been implicated in a wide array of cellular processes including cell division, cell adhesion, regulation of calcium activity, and tumorigenesis, particularly in neuronal cells. However, their expression patterns during early development remain largely unknown. Here, we describe the spatial and temporal patterning of ttyh1, ttyh2, and ttyh3 in Xenopus laevis during early embryonic development. Ttyh1 and ttyh3 are initially expressed at the late neurula stage are and primarily localized to the developing nervous system; however ttyh1 and ttyh3 both show transient expression in the somites...
January 2015: Gene Expression Patterns: GEP
#32
JOURNAL ARTICLE
Amal Said Darweesh, Manal Louis Louka, Maha Hana, Shaymaa Rashad, Mohamed El-Shinawi, Ahmed Sharaf-Eldin, Samar Kamal Kassim
A previously reported microarray data analysis by RISS algorithm on breast cancer showed over-expression of the growth factor receptor (Grb7) and it also highlighted Tweety (TTYH1) gene to be under expressed in breast cancer for the first time. Our aim was to validate the results obtained from the microarray analysis with respect to these genes. Also, the relationship between their expression and the different prognostic indicators was addressed. RNA was extracted from the breast tissue of 30 patients with primary malignant breast cancer...
October 2014: Medical Oncology
#33
JOURNAL ARTICLE
Madeleine Pritchard, Naomi Cocks, Lucy Dipper
Although there is a substantive body of research about the language used by individuals with aphasia, relatively little is known about their spontaneous iconic gesture. A single case study of LT, an individual with conduction aphasia indicated qualitative differences between the spontaneous iconic gestures produced alongside fluent speech and in tip of the tongue states. The current study examined the iconic gestures produced by another individual with conduction aphasia, WT, and a group of 11 control participants...
October 2013: International Journal of Speech-language Pathology
#34
JOURNAL ARTICLE
Marzena Stefaniuk, Lukasz Swiech, Joanna Dzwonek, Katarzyna Lukasiuk
We have previously shown that Ttyh1 mRNA is expressed in neurons and its expression is up-regulated in the brain during epileptogenesis and epilepsy. In this study, we aimed to elucidate the role of Ttyh1 in neurons. We found widespread expression of Ttyh1 protein in neurons in vivo and in vitro. Ttyh1 immunoreactivity in vitro was frequently found in invaginations of dendritic spines; however, Ttyh1, seldom co-localized with synaptic markers in vivo. Silencing Ttyh1 expression with siRNA in hippocampal cultures resulted in alterations of MAP2 distribution along neurites causing it to appear in the form of chains of beads...
December 2010: Journal of Neurochemistry
#35
JOURNAL ARTICLE
Marzena Stefaniuk, Katarzyna Lukasiuk
The goal of this study was to clone and sequence selected expressed sequence tags (ESTs) which mRNA is upregulated in a rat model of epilepsy based on our previous work [17] and to determine the localization of their mRNA expression in the normal brain. Six ESTs that had not been assigned to any gene at the time of the inception of our experiments were chosen for analysis. Radioactive in situ hybridization revealed that the expression of four transcripts (AA955087, AA875438, AA899079, AA819660) was clearly localized to hippocampal neurons and was also detected in the cortex...
October 4, 2010: Neuroscience Letters
#36
JOURNAL ARTICLE
Tomohiro Kumada, Yasunari Yamanaka, Ayumi Kitano, Minoru Shibata, Tomonari Awaya, Takeo Kato, Katsuya Okawa, Takaya Abe, Naoko Oshima, Tatsutoshi Nakahata, Toshio Heike
Using comprehensive genetic studies on neuronal stem/progenitors cells through genome-wide screening with oligonucleotide arrays, we identified an endoplasmic reticulum (ER) -resident protein, Tweety homologue 1 (ttyh1). Ttyh1 encodes a glycosylated protein composed of five predicted transmembrane segments and a C-terminus that is enriched in negatively charged residues capable of Ca(2+) binding. Ttyh1-containing membranes changed to segmented tubuloreticular structures during mitosis, suggesting that the ER-containing Ttyh1 could be responsible for Ca(2+) sequestration and Ca(2+) concentration regulation during mitosis...
August 2010: Developmental Dynamics
#37
JOURNAL ARTICLE
Marine Henry, Orla O'Sullivan, Roy D Sleator, Aidan Coffey, R Paul Ross, Olivia McAuliffe, Jim M O'Mahony
Ardmore is a novel mycobacteriophage isolated from a soil sample collected in County Waterford, Ireland. The genome of this phage has been fully sequenced and is composed of 52,141 bp of linear double stranded DNA with a GC content of 61.49%. 88 ORFs were identified of which 34 were assigned predicted functions based on their homology to previously characterised proteins, their location in the genome, computer-predicted structural characteristics or presence of conserved motifs in their sequence. The Ardmore genome appears highly similar to mycobacteriophages Fruitloop and Tweety with BLASTn analysis showing 87% and 80% identity respectively...
March 15, 2010: Gene
#38
JOURNAL ARTICLE
Mathieu Boudes, Chamroeun Sar, Aurélie Menigoz, Cécile Hilaire, Marie O Péquignot, Alexei Kozlenkov, Alan Marmorstein, Patrick Carroll, Jean Valmier, Frédérique Scamps
We investigated the molecular determinants of Ca(2+)-activated chloride current (CaCC) expressed in adult sensory neurons after a nerve injury. Dorsal root ganglia express the transcripts of three gene families known to induce CaCCs in heterologous systems: bestrophin, tweety, and TMEM16. We found with quantitative transcriptional analysis and in situ hybridization that nerve injury induced upregulation of solely bestrophin-1 transcripts in sensory neurons. Gene screening with RNA interference in single neurons demonstrated that mouse Best1 is required for the expression of CaCC in injured sensory neurons...
August 12, 2009: Journal of Neuroscience
#39
JOURNAL ARTICLE
Marco Morciano, Tobias Beckhaus, Michael Karas, Herbert Zimmermann, Walter Volknandt
The presynaptic proteome controls neurotransmitter release and the short and long term structural and functional dynamics of the nerve terminal. Using a monoclonal antibody against synaptic vesicle protein 2 we immunopurified a presynaptic compartment containing the active zone with synaptic vesicles docked to the presynaptic plasma membrane as well as elements of the presynaptic cytomatrix. Individual protein bands separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis were subjected to nanoscale-liquid chromatography electrospray ionization-tandem mass spectrometry...
February 2009: Journal of Neurochemistry
#40
JOURNAL ARTICLE
Yaowu He, Deanne H Hryciw, Melanie L Carroll, Stephen A Myers, Astrid K Whitbread, Sharad Kumar, Philip Poronnik, John D Hooper
The Tweety proteins comprise a family of chloride ion channels with three members identified in humans (TTYH1-3) and orthologues in fly and murine species. In humans, increased TTYH2 expression is associated with cancer progression, whereas fly Tweety is associated with developmental processes. Structurally, Tweety proteins are characterized by five membrane-spanning domains and N-glycan modifications important for trafficking to the plasma membrane, where these proteins are oriented with the amino terminus located extracellularly and the carboxyl terminus cytoplasmically...
August 29, 2008: Journal of Biological Chemistry
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