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https://www.readbyqxmd.com/read/28535303/a-novel-goldnano-carb-test-for-rapid-phenotypic-detection-of-carbapenemases-particularly-oxa-type-in-enterobacteriaceae-pseudomonas-aeruginosa-and-acinetobacter-spp
#1
Arpasiri Srisrattakarn, Aroonlug Lulitanond, Chotechana Wilailuckana, Nicha Charoensri, Jureerut Daduang, Aroonwadee Chanawong
Objectives: To develop a simple gold nanoparticle (AuNP)-based colorimetric test, GoldNano Carb (GoldC), for detecting carbapenemase production in Gram-negative bacteria, compared with updated Carba NP (CNP) and CarbAcineto NP (CAcNP) tests by using PCR methods as gold standard. Methods: Ninety-nine carbapenemase-producing Enterobacteriaceae (CPE), Pseudomonas spp. and Acinetobacter spp. isolates and 89 non-CPE isolates were tested by the GoldC and CNP. Additionally, the CAcNP was performed in the Acinetobacter spp...
May 23, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/28396492/the-geometric-framework-for-nutrition-reveals-interactions-between-protein-and-carbohydrate-during-larval-growth-in-honey-bees
#2
Bryan R Helm, Garett P Slater, Arun Rajamohan, George D Yocum, Kendra J Greenlee, Julia H Bowsher
In holometabolous insects, larval nutrition affects adult body size, a life history trait with a profound influence on performance and fitness. Individual nutritional components of larval diets are often complex and may interact with one another, necessitating the use of a geometric framework for elucidating nutritional effects. In the honey bee, Apis mellifera, nurse bees provision food to developing larvae, directly moderating growth rates and caste development. However, the eusocial nature of honey bees makes nutritional studies challenging, because diet components cannot be systematically manipulated in the hive...
June 15, 2017: Biology Open
https://www.readbyqxmd.com/read/28279669/contribution-of-visuospatial-attention-short-term-memory-and-executive-functions-to-performance-in-number-interval-bisection
#3
Mariagrazia Ranzini, Katia Carbè, Wim Gevers
Number interval bisection consists of estimating the mid-number within a pair (1-9=>5). Healthy adults and right-brain damage patients can show biased performance in this task, underestimating and overestimating the mid-number, respectively. The role of visuospatial attention during this task, and its interplay with other cognitive abilities (e.g., working memory) is still object of debate. In this study we explored the relation between visuospatial attention and individual differences in working memory and executive functions during number interval bisection...
March 6, 2017: Neuropsychologia
https://www.readbyqxmd.com/read/28254873/inter-and-intra-observer-agreement-of-fdg-pet-ct-image-interpretation-in-patients-referred-for-assessment-of-cardiac-sarcoidosis
#4
Hiroshi Ohira, Brian Mc Ardle, Robert A deKemp, Pablo B Nery, Daniel Juneau, Jennifer M Renaud, Ran Klein, Owen Clarkin, Karen MacDonald, Eugene Leung, Girish Nair, Rob S B Beanlands, David Birnie
Recent studies have reported the usefulness of (18)F-fluoro-2-deoxyglucose positron emission tomography (FDG-PET) in aiding with the diagnosis and management of patients with cardiac sarcoidosis (CS). However, the image interpretation of FDG-PET for CS is sometimes challenging. We sought to investigate the inter- and intra-observer agreement and explore factors which lead to important discrepancies between readers. METHODS: We studied consecutive patients with no significant coronary artery disease who were referred for assessment of CS...
March 2, 2017: Journal of Nuclear Medicine: Official Publication, Society of Nuclear Medicine
https://www.readbyqxmd.com/read/28148458/impact-of-biodiesel-on-regulated-and-unregulated-emissions-and-redox-and-proinflammatory-properties-of-pm-emitted-from-heavy-duty-vehicles
#5
Georgios Karavalakis, Nicholas Gysel, Debra A Schmitz, Arthur K Cho, Constantinos Sioutas, James J Schauer, David R Cocker, Thomas D Durbin
The emissions and the potential health effects of particulate matter (PM) were assessed from two heavy-duty trucks with and without emission control aftertreatment systems when operating on CARB ultra-low sulfur diesel (ULSD) and three different biodiesel blends. The CARB ULSD was blended with soy-based biodiesel, animal fat biodiesel, and waste cooking oil biodiesel at 50vol%. Testing was conducted over the EPA Urban Dynamometer Driving Schedule (UDDS) in triplicate for both trucks. The aftertreatment controls effectively decreased PM mass and number emissions, as well as the polycyclic aromatic hydrocarbons (PAHs) compared to the uncontrolled truck...
April 15, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28050905/detailed-analysis-of-criteria-and-particle-emissions-from-a-very-large-crude-carrier-using-a-novel-eca-fuel
#6
Nicholas R Gysel, William A Welch, Kent Johnson, Wayne Miller, David R Cocker
Ocean going vessels (OGVs) operating within emission control areas (ECA) are required to use fuels with ≤0.1 wt % sulfur. Up to now only distillate fuels could meet the sulfur limits. Recently refiners created a novel low-sulfur heavy-fuel oil (LSHFO) meeting the sulfur limits so questions were posed whether nitric oxide (NOx) and particulate matter (PM) emissions were the same for the two fuels. This project characterized criteria pollutants and undertook a detailed analysis of PM emissions from a very large crude oil carrier (VLCC) using a distillate ECA fuel (MGO) and novel LSHFO...
February 7, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/27737785/detection-of-carbapenemase-activity-in-enterobacteriaceae-using-lc-ms-ms-in-comparison-with-the-neo-rapid-carb-kit-using-direct-visual-assessment-and-colorimetry
#7
Charlotte A Huber, Hanna E Sidjabat, Hosam M Zowawi, David Kvaskoff, Sarah Reed, John F McNamara, Kate L McCarthy, Patrick Harris, Benjamin Toh, Alexander M Wailan, David L Paterson
It has been described that the sensitivity of the Carba NP test may be low in the case of OXA-48-like carbapenamases and mass spectrometry based methods as well as a colorimetry based method have been described as alternatives. We evaluated 84 Enterobacteriaceae isolates including 31 OXA-48-like producing isolates and 13 isolates that produced either an imipenemase (IMP; n=8), New Delhi metallo-β-lactamase (NDM; n=3), or Klebsiella pneumoniae carbapenemase (KPC; n=2), as well as 40 carbapenemase negative Enterobacteriaceae isolates...
October 11, 2016: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/27733250/a-human-model-of-dietary-saturated-fatty-acid-induced-insulin-resistance
#8
RANDOMIZED CONTROLLED TRIAL
Juraj Koska, Marlies K Ozias, James Deer, Julie Kurtz, Arline D Salbe, S Mitchell Harman, Peter D Reaven
BACKGROUND: Increased consumption of high-fat diets is associated with the development of insulin resistance and type 2 diabetes. Current models to study the mechanisms of high-fat diet-induced IR in humans are limited by their long duration or low efficacy. In the present study we developed and characterized an acute dietary model of saturated fatty acid-enriched diet induced insulin resistance. METHODS: High caloric diets enriched with saturated fatty acids (SFA) or carbohydrates (CARB) were evaluated in subjects with normal and impaired glucose tolerance (NGT or IGT)...
November 2016: Metabolism: Clinical and Experimental
https://www.readbyqxmd.com/read/27494448/long-term-effects-of-weight-loss-with-a-very-low-carbohydrate-low-saturated-fat-diet-on-flow-mediated-dilatation-in-patients-with-type-2-diabetes-a-randomised-controlled-trial
#9
Thomas P Wycherley, Campbell H Thompson, Jonathan D Buckley, Natalie D Luscombe-Marsh, Manny Noakes, Gary A Wittert, Grant D Brinkworth
BACKGROUND AND AIMS: Very-low carbohydrate diets can improve glycaemic control in patients with type 2 diabetes (T2DM). However, compared to traditional higher carbohydrate, low fat (HighCHO) diets, they have been associated with impaired endothelial function (measured by flow mediated dilatation [FMD]) that is possibly related to saturated fat. This study aimed to examine the effects of a 12-month hypocaloric very-low carbohydrate, low saturated fat (LowCHO) diet compared to an isocaloric HighCHO diet...
September 2016: Atherosclerosis
https://www.readbyqxmd.com/read/27490353/-characteristics-of-nutrition-in-competitive-sports-ranging-from-leisure-activities-to-high-performance-athletics
#10
REVIEW
H Braun
Nutrition has a crucial influence on physical and mental performance ability and is an important measure along sidetraining in high-performance athletes. However, this form of nutritionis not applicable for every athlete and in every situation. The question of optimal nutrition requires involvement with the particular type of sports, an athlete's current training stage, and athletes' individual requirements and objectives. Implementation takes time and individual motivation on the part of athletes and the specialist staff who engage intensively with the nutritional needs of athletes...
August 2016: Sportverletzung Sportschaden: Organ der Gesellschaft Für Orthopädisch-Traumatologische Sportmedizin
https://www.readbyqxmd.com/read/27342793/determination-of-endocrine-disrupting-compounds-in-fish-liver-brain-and-muscle-using-focused-ultrasound-solid-liquid-extraction-and-dispersive-solid-phase-extraction-as-clean-up-strategy
#11
Oihana Ros, Asier Vallejo, Maitane Olivares, Nestor Etxebarria, Ailette Prieto
This study describes a new method for the simultaneous extraction of several endocrine disrupting compounds, including alkylphenols (APs), estrogen, bisphenol-A (BPA) and one phthalate metabolite (mono-2-ethylhexyl ester, MEHP) in fish liver, brain, and muscle. Parameters affecting the extraction (extraction solvent and temperature) and the clean-up (dispersive phase nature and amount) steps were evaluated. The extraction was performed by means of focused ultrasound solid-liquid extraction (FUSLE) using 10 mL of n-hexane:acetone (50:50, v/v) for 5 min at ~0 °C, and the clean-up was done by means of dispersive solid phase extraction (dSPE) using 100 mg of ENVI-CARB and 100 mg of MgSO4 for the cleaning of brain and muscle extracts together with 100 mg of PSA in the case of liver extracts...
August 2016: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/27161762/evaluation-of-the-methylene-blue-addition-in-binary-polymeric-systems-composed-by-poloxamer-407-and-carbopol-934p-using-quality-by-design-rheological-textural-and-mucoadhesive-analysis
#12
Mariana Volpato Junqueira, Fernanda Belincanta Borghi-Pangoni, Sabrina Barbosa de Souza Ferreira, Marcos Luciano Bruschi
This study describes the investigation about the physicochemical behavior of methylene blue (Mb) addition to systems containing poloxamer 407 (Polox), Carbopol 934P (Carb), intended to be locally used by photodynamic therapy. A factorial design 2(3) (plus center point) was used to analyze the rheological, mucoadhesive and textural properties of the preparations. Systems containing the lower concentrations of Polox (15 and 17.5%, w/w) exhibited pseudoplastic flow and low degrees of rheopexy. On the other hand, at higher Polox concentration (20%, w/w) the systems display plastic flow and thixotropy...
May 10, 2016: Drug Development and Industrial Pharmacy
https://www.readbyqxmd.com/read/26553353/screening-and-in-vitro-evaluation-of-mucoadhesive-thermoresponsive-system-containing-methylene-blue-for-local-photodynamic-therapy-of-colorectal-cancer
#13
Fernanda Belincanta Borghi-Pangoni, Mariana Volpato Junqueira, Sabrina Barbosa de Souza Ferreira, Larissa Lachi Silva, Bruno Ribeiro Rabello, Wilker Caetano, Andrea Diniz, Marcos Luciano Bruschi
PURPOSE: Photodynamic therapy (PDT) with methylene blue (MB) constitutes a potentially useful modality for colorectal cancer treatment. The limitations of the formulations containing MB are problems of administration and the inability to get the closeness contact at the site during the appropriate residence time. Present study aimed to develop and characterize mucoadhesive thermoresponsive system containing MB designed as platform for colorectal cancer therapy. METHODS: Formulations composed of different amounts of poloxamer 407 (Polox), Carbopol 934P (Carb), and MB were developed and characterized as rheological, compressional, mucoadhesive and syringeability properties, toxicity, photodynamic action, in vitro MB release profile, and ex vivo MB intestinal permeation...
March 2016: Pharmaceutical Research
https://www.readbyqxmd.com/read/26487764/dietary-intake-in-swedish-medical-students-during-2007-2012
#14
Ellinor Fredriksson, Hilde Kristin Brekke, Lars Ellegård
BACKGROUND: The dietary intake in Swedish medical students has been reported for the periods 1987-1993 and 1994-2006. OBJECTIVE: To analyse dietary intake in medical students between 2007 and 2012, in relation to Nordic Nutrition Recommendations, to previous surveys and to a contemporary Swedish population. DESIGN: Nutrient intake was calculated from 3-day food records conducted by 698 medical students. The differences between surveys were evaluated using a t-test and the changes over time by linear regression...
February 2016: Scandinavian Journal of Public Health
https://www.readbyqxmd.com/read/26452864/new-approach-for-assessing-human-perfluoroalkyl-exposure-via-hair
#15
Andreia Alves, Griet Jacobs, Guido Vanermen, Adrian Covaci, Stefan Voorspoels
In the recent years hair has been increasingly used as alternative matrix in human biomonitoring (HBM) of environmental pollutants. Sampling advantages and time integration of exposure assessment seems the most attractive features of hair matrix. In the current study, a novel miniaturized method was developed and validated for measuring 15 perfluoroalkyl substances (PFAS), including perfluoro n-butanoic acid (PFBA), perfluoro n-pentanoic acid (PFPeA), perfluoro n-hexanoic acid (PFHxA), perfluoro n-heptanoic acid (PFHpA), perfluor n-octanoic acid (PFOA), perfluoro n-nonanoic acid (PFNA), perfluoro tetradecanoic acid (PFTeDA), perfluorobutane sulfonic acid (PFBS), perfluoro pentane sulfonic acid (PFPeS), perfluorohexane sulfonic acid (PFHxS), perfluoroheptane sulfonic acid (PFHpS), perfluorooctane sulfonic acid (PFOS), perfluorononane sulfonic acid (PFNS), perfluorodecane sulfonic acid (PFDS) and perfluorododecane sulfonic acid (PFDoS) in human hair by liquid chromatography tandem mass spectrometry (LC-MS/MS)...
November 1, 2015: Talanta
https://www.readbyqxmd.com/read/26379200/ecological-risks-of-home-and-personal-care-products-in-the-riverine-environment-of-a-rural-region-in-south-china-without-domestic-wastewater-treatment-facilities
#16
Nai-Sheng Zhang, You-sheng Liu, Paul J Van den Brink, Oliver R Price, Guang-Guo Ying
Home and personal care products (HPCPs) including biocides, benzotriazoles (BTs) and ultraviolet (UV) filters are widely used in our daily life. After use, they are discharged with domestic wastewater into the receiving environment. This study investigated the occurrence of 29 representative HPCPs, including biocides, BTs and UV filters, in the riverine environment of a rural region of South China where no wastewater treatment plants were present, and assessed their potential ecological risks to aquatic organisms...
December 2015: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/26333511/the-low-fat-low-carb-debate-and-the-theory-of-relativity
#17
EDITORIAL
Caroline M Apovian
No abstract text is available yet for this article.
October 2015: American Journal of Clinical Nutrition
https://www.readbyqxmd.com/read/26300227/polyphenolic-composition-of-grape-stem-extracts-affects-antioxidant-activity-in-endothelial-and-muscle-cells
#18
Nikolaos Goutzourelas, Dimitrios Stagos, Ypatios Spanidis, Maria Liosi, Anna Apostolou, Alexandros Priftis, Serko Haroutounian, Demetrios A Spandidos, Aristidis M Tsatsakis, Demetrios Kouretas
The aim of the present study was the assessment of the antioxidant effects of polyphenolic extracts derived from the stems of three Greek grape varieties (Moshomayro, Mavrotragano and Mandilaria) in endothelial (EA.hy926) and muscle (C2C12) cells. We also investigated the effects of the polyphenolic composition on the antioxidant effects of the grape stem extracts. For this purpose, the endothelial and muscle cells were treated with low non-cytotoxic concentrations of the extracts for 24 h in order to assess the effects of the extracts on cellular redox status using oxidative stress biomarkers...
October 2015: Molecular Medicine Reports
https://www.readbyqxmd.com/read/26279863/crystal-structures-of-two-6-2-hy-droxy-benzo-yl-5h-thia-zolo-3-2-a-pyrimidin-5-ones
#19
Ligia R Gomes, John Nicolson Low, Fernando Cagide, Fernanda Borges
The title compounds, 6-(2-hy-droxy-benz-yl)-5H-thia-zolo[3,2-a]pyrimidin-5-one, C13H8N2O3S, (1), and 6-(2-hy-droxy-benz-yl)-3-methyl-5H-thia-zolo[3,2-a]pyrimidin-5-one, C14H10N2O3S, (2), were synthesized when a chromone-3-carb-oxy-lic acid, activated with (benzotriazol-1-yl-oxy)tripyrrolidinyl-phospho-nium hexa-fluorido-phosphate (PyBOP), was reacted with a primary heteromamine. Instead of the expected amidation, the unusual title thia-zolo-pyrimidine-5-one derivatives were obtained serendipitously and a mechanism of formation is proposed...
July 1, 2015: Acta Crystallographica. Section E, Crystallographic Communications
https://www.readbyqxmd.com/read/26223127/-therapeutic-effects-and-mechanisms-of-opuntia-dillenii-haw-on-atherosclerosis-of-rats
#20
Yu-chun Wang, Zhan-peng Qi, Zhen-zhong Liu, Tao Li, Hong-xia Cui, Bao-qing Wang, Na Chi
The research aimed to investigate the therapeutic effects and mechanisms of Opuntia dillenii Haw polysaccharide (OPS) on atherosclerosis of rats. First atherosclerotic rat models were established by high-fat and high-calcium diet. Thirty days later, the rats were treated with low dosage of OPS (0.2 g x kg(-1) x d(-1)) or high dosage of OPS (0.4 g x kg(-1) x d(-1)) by intraperitoneal injection for 60 days continuously. At the end of treatment, thoracic aorta rings were prepared and vasorelaxation of rat thoracic aorta in different experiment groups were determined by using 620M multi wire myograph system in vitro...
April 2015: Yao Xue Xue Bao, Acta Pharmaceutica Sinica
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