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https://www.readbyqxmd.com/read/28110519/examining-the-nutritional-quality-of-food-and-beverage-consumed-at-melbourne-aquatic-and-recreation-centres
#1
Tara Boelsen-Robinson, Alexandra Chung, Marianne Khalil, Evelyn Wong, Ariana Kurzeme, Anna Peeters
OBJECTIVE: Examine the nutritional quality of food and beverages consumed across a sample of community aquatic and recreation centres in metropolitan Melbourne, Australia. METHODS: Interviewer-administered surveys of randomly selected patrons attending four aquatic and recreation centres were conducted to ascertain food and beverage items consumed over two data collection periods (May-June 2014, January-February 2015). We selected centres in and around metropolitan Melbourne with a sit-down cafeteria and children's swimming classes...
January 22, 2017: Australian and New Zealand Journal of Public Health
https://www.readbyqxmd.com/read/28110486/spectra-optia-%C3%A2-for-automated-red-blood-cell-exchange-in-patients-with-sickle-cell-disease-a-nice-medical-technology-guidance
#2
REVIEW
Iain Willits, Helen Cole, Roseanne Jones, Kimberley Carter, Mick Arber, Michelle Jenks, Joyce Craig, Andrew Sims
The Spectra Optia(®) automated apheresis system, indicated for red blood cell exchange in people with sickle cell disease, underwent evaluation by the National Institute for Health and Care Excellence, which uses its Medical Technologies Advisory Committee to make recommendations. The company (Terumo Medical Corporation) produced a submission making a case for adoption of its technology, which was critiqued by the Newcastle and York external assessment centre. Thirty retrospective observational studies were identified in their clinical submission...
January 21, 2017: Applied Health Economics and Health Policy
https://www.readbyqxmd.com/read/28110314/long-term-exposure-to-a-butter-rich-diet-induces-mild-to-moderate-steatosis-in-chang-liver-cells-and-swiss-albino-mice-models
#3
Thomas John Philip Nalloor, Nitesh Kumar, Kasinathan Narayanan, Vasanth Raj Palanimuthu
BACKGROUND: Butter is one of the widely used fats present in the diet. However, there is no satisfactory study available that evaluates the effect of a high-fat diet containing butter as the principal fat on the development of non-alcoholic fatty liver disease (NAFLD). METHODS: In the present study, butter was used for the development of steatosis in Chang liver cells in an in vitro study and Swiss albino mice in an in vivo study. In vitro steatosis was established, and butter was compared with oleic acid in Chang liver cells using an oil red O (ORO)-based colorimetric assay...
January 23, 2017: Journal of Basic and Clinical Physiology and Pharmacology
https://www.readbyqxmd.com/read/28110127/bacteriophage-application-on-red-meats-and-poultry-effects-on-salmonella-population-in-final-ground-products
#4
Y Yeh, P Purushothaman, N Gupta, M Ragnone, S C Verma, A S de Mello
This research was conducted to study the effects of bacteriophage application during tumbling on Salmonella populations in ground meat and poultry. Red meat trim and poultry were inoculated with a Salmonella cocktail to result in a contamination level of 7logCFU/g in ground products. A commercial preparation containing bacteriophages S16 and Felix-O1a (FO1a) was applied during tumbling at 10(7) and 10(8)PFU/ml. Samples were held at 4°C for 6h and 18h (red meat) and 30min and 6h (poultry). Overall, bacteriophage application on trim reduced 1 and 0...
January 9, 2017: Meat Science
https://www.readbyqxmd.com/read/28109844/colorimetric-detection-of-cucumber-green-mottle-mosaic-virus-using-unmodified-gold-nanoparticles-as-colorimetric-probes
#5
Lin Wang, Zhanmin Liu, Xueying Xia, Cuiyun Yang, Junyi Huang, Sibao Wan
Cucumber green mottle mosaic virus (CGMMV)causes a severe mosaic symptom of watermelon and cucumber, and can be transmitted via infected cucumber seeds, leaves and soil. It remains a challenge to detect this virus to prevent its introduction and infection and spread in fields. For this purpose, a simple and sensitive label-free colorimetric detection method for CGMMV has been developed with unmodified gold nanoparticles (AuNPs) as colorimetric probes. The method is based on the finding that the presence of RT-PCR target products of CGMMV and species-specific probes results in color change of AuNPs from red to blue after NaCl induction...
January 18, 2017: Journal of Virological Methods
https://www.readbyqxmd.com/read/28109837/interspecific-variation-and-plasticity-in-hemoglobin-nitrite-reductase-activity-and-its-correlation-with-oxygen-affinity-in-vertebrates
#6
Frank B Jensen, Rasmus A H Kolind, Natashia S Jensen, Gabriella Montesanti, Tobias Wang
Deoxygenated hemoglobin (Hb) is a nitrite reductase that reduces naturally occurring nitrite to nitric oxide (NO), supplying physiological relevant NO under hypoxic conditions. The nitrite reductase activity is modulated by the allosteric equilibrium between the R and T structures of Hb that also determines oxygen affinity. In the present study we investigated nitrite reductase activity and O2 affinity in Hbs from ten different vertebrate species under identical conditions to disclose interspecific variations and allow an extended test for a correlation between the rate constant for nitrite reduction and O2 affinity...
January 18, 2017: Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology
https://www.readbyqxmd.com/read/28109601/effects-of-rumen-protected-capsicum-oleoresin-on-immune-responses-in-dairy-cows-intravenously-challenged-with-lipopolysaccharide
#7
J Oh, M Harper, F Giallongo, D M Bravo, E H Wall, A N Hristov
The objective of this experiment was to investigate the effects of rumen-protected Capsicum oleoresin (RPC) on productivity and immune responses including feed intake, milk yield and composition, white and red blood cells, lipid peroxidation, and blood concentration of cortisol, haptoglobin, glucose, and insulin in lactating dairy cows experimentally challenged with lipopolysaccharide (LPS). The experiment was a replicated 3 × 3 Latin square design with 9 multiparous Holstein cows in three 28-d periods. Treatments were 0 (control), 100, and 200 mg of RPC/cow per day, mixed with small portions of the total mixed ration and top-dressed...
January 18, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28109460/dietary-protein-sources-and-all-cause-and-cause-specific-mortality-the-golestan-cohort-study-in-iran
#8
Maryam S Farvid, Akbar F Malekshah, Akram Pourshams, Hossein Poustchi, Sadaf G Sepanlou, Maryam Sharafkhah, Masoud Khoshnia, Mojtaba Farvid, Christian C Abnet, Farin Kamangar, Sanford M Dawsey, Paul Brennan, Paul D Pharoah, Paolo Boffetta, Walter C Willett, Reza Malekzadeh
INTRODUCTION: Dietary protein comes from foods with greatly different compositions that may not relate equally with mortality risk. Few cohort studies from non-Western countries have examined the association between various dietary protein sources and cause-specific mortality. Therefore, the associations between dietary protein sources and all-cause, cardiovascular disease, and cancer mortality were evaluated in the Golestan Cohort Study in Iran. METHODS: Among 42,403 men and women who completed a dietary questionnaire at baseline, 3,291 deaths were documented during 11 years of follow up (2004-2015)...
February 2017: American Journal of Preventive Medicine
https://www.readbyqxmd.com/read/28109163/an-assessment-of-the-overexpression-of-bmp-2-in-transfected-human-osteoblast-cells-stimulated-by-mta-and-biodentine
#9
E M Rodrigues, A L G Cornélio, A S C Fuentes, L P Salles, M Velayutham, C Rossa-Junior, J M Guerreiro-Tanomaru, M Tanomaru-Filho
AIM: To evaluate the effect of MTA and Biodentine on viability, osteogenic differentiation and BMP-2 expression in osteogenic cells. METHODOLOGY: Saos-2 cells were used as a model of osteoblastic cells. Overexpression of BMP2 was induced by transfection of a CMV-driven plasmid construct including the human BMP-2 coding sequence and stably transfected cells were selected. Cell viability was assessed by the mitochondrial dehydrogenase enzymatic (MTT) assay. The bioactivity of the materials was evaluated by the Alkaline Phosphatase (ALP) assay and detection of calcium deposits with Alizarin Red Staining (ARS)...
January 21, 2017: International Endodontic Journal
https://www.readbyqxmd.com/read/28109150/-isolation-culture-and-identification-of-adipose-derived-stem-cells-from-sd-rat-adipose-tissues-subjected-to-long-term-cryopreservation
#10
Qin Liu, Liping Wang, Fang Chen, Yi Zhang
Objective To study the feasibility of isolation and culture of adipose-derived stem cells (ADSCs) from SD rat adipose tissues subjected to long-term cryopreservation. Methods We took inguinal fat pads from healthy SD rats. Adipose tissues were stored with 100 mL/L dimethyl sulfoxide (DMSO) combined with 900 mL/L fetal bovine serum (FBS) in liquid nitrogen. Three months later, the adipose tissues were resuscitated for the isolation and culture of ADSCs. The growth status and morphology were observed. The growth curve and cell surface markers CD29, CD45, CD90 of the 3rd passage cells were analyzed respectively by CCK-8 assay and immunocytochemistry...
February 2017: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
https://www.readbyqxmd.com/read/28109148/-rat-bone-marrow-mesenchymal-stem-cells-promote-apoptosis-and-inhibit-proliferation-and-migration-of-rsc96-cells
#11
Yongbin Ma, Suohua Ge, Dan Zhou, Huanyan Zhang, Luyang Sun, Xuefeng Wang, Jianhua Su
Objective To investigate the effect of rat bone marrow mesenchymal stem cells (BMSCs) on the proliferation and migration of rat RSC96 Schwann cells in vitro. Methods BMSCs of Sprague Dawley (SD) rats were isolated by bone marrow cell adherent method. The morphology of the third generation of BMSCs was observed by inverted microscopy. The cell surface antigen makers CD19, CD34, CD73, CD105 were detected by flow cytometry. The third-passage BMSCs differentiated into osteoblasts and adipocytes after exposed to bone-inducing and lipid-inducing media for 3 and 2 weeks, respectively, and then were identified by oil red O and alkaline phosphatase staining...
February 2017: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
https://www.readbyqxmd.com/read/28109111/-efficacy-of-high-versus-low-plasma-red-blood-cell-ratio-resuscitation-in-patients-with-severe-trauma-requiring-massive-blood-transfusion-a-meta-analysis
#12
Fang Yu, Tao Zhong, Gang Wu
OBJECTIVE: To evaluate the efficacy of high (≥1:2) and low (<1:2) plasma: red blood cell (RBC) ratio resuscitation in patients with severe trauma requiring massive blood transfusion. METHODS: The databases including the Cochrane Library, Pubmed, Web of Science, and EMBASE were systemically searched for relevant studies published between January, 2009 and April, 2016. The selection of studies, assessment of methodological quality and data extraction were performed by two researchers independently according to the inclusion and exclusion criteria...
January 20, 2017: Nan Fang Yi Ke da Xue Xue Bao, Journal of Southern Medical University
https://www.readbyqxmd.com/read/28109101/-fgf-2-pela-bmp-2-microcapsule-scaffold-promotes-osteogenic-differentiation-of-rat-periosteum-derived-stem-cells-in-vitro
#13
Jie Yin, Su-Jun Qiu, Jun-Huai Gao, Sheng-Li Zhao, Shao-Xiong Min
OBJECTIVE: To observe the effect of a microencapsule scaffold capable of sustained release of fibroblast growth factor-2 (FGF-2) and bone morphogenetic protein-2 (BMP-2) in promoting the osteogenic differentiation of rat periosteum-derived stem cells (PDSCs) in vitro. METHODS: PDSCs from 4-week-old SD rats, after identification of the surface markers using flow cytometry, were induced to differentiate into osteoblast, chondroblast, and adipocyte lineages. The differentiated cells were verified by staining with Alizarin red, toluidine blue, alcian blue, oil red O and by immunofluorescence assay...
January 20, 2017: Nan Fang Yi Ke da Xue Xue Bao, Journal of Southern Medical University
https://www.readbyqxmd.com/read/28109081/shope-fibroma-in-the-external-ear-canal-of-a-domestic-rabbit
#14
Shawna J
A 5-y-old, intact, 2.5-kg female domestic rabbit was presented because of blood spatter on the wall of its cage and thetoenailsof its right hind limb. Physical examination revealed a red, gelatinous mass that spanned the width of the right vertical ear canal. Radiographic images revealed a soft-tissue opacity at the base of the right ear, which was superimposed over the tympanic bulla and extended to the pinna. A CT scan revealed that the soft-tissue mass was within the vertical and horizontal portions of the right external ear canal and extended to the level of the tympanic membrane, with no bony involvement...
January 20, 2017: Comparative Medicine
https://www.readbyqxmd.com/read/28108999/differential-response-of-scots-pine-seedlings-to-variable-intensity-and-ratio-of-r-and-fr-light
#15
Md Abdur Razzak, Sonali Sachin Ranade, Åsa Strand, M R García-Gil
We investigated the response to increasing intensity of red (R) and far-red (FR) light and to a decrease in R:FR ratio in Pinus sylvestris L. (Scots pine) seedling. The results showed that FR-high irradiance response (FR-HIR) for hypocotyl elongation may be present in Scots pine, and that this response is enhanced by increasing light intensity. However, both hypocotyl inhibition and pigment accumulation were more strongly affected by the R light compared to FR light. This is in contrast to previous reports in Arabidopsis thaliana (L...
January 20, 2017: Plant, Cell & Environment
https://www.readbyqxmd.com/read/28108956/post-implantation-fate-of-adipogenic-induced-mesenchymal-stem-cells-on-type-i-collagen-scaffold-in-a-rat-model
#16
Balu Venugopal, Francis B Fernandez, V S Harikrishnan, Annie John
Regenerative medicine via its application in soft tissue reconstruction through novel methods in adipose tissue engineering (ATE) has gained remarkable attention and investment despite simultaneous reports on clinical incidence of graft resorption and impaired vascularization. The underlying malaise here once identified may play a critical role in optimizing implant function. Our work attempts to determine the fate of donor cells and the implant in recipient micro environment using adipose-derived mesenchymal stem cells (ASCs) on a type I collagen sponge, an established scaffold for ATE...
February 2017: Journal of Materials Science. Materials in Medicine
https://www.readbyqxmd.com/read/28108825/photodynamic-therapy-for-the-treatment-of-complex-anal-fistula
#17
A Arroyo, P Moya, M A Rodríguez-Prieto, M J Alcaide, M M Aguilar, M Bellón, M T Pérez-Vázquez, F Candela, R Calpena
BACKGROUND: Photodynamic therapy (PDT) is a new procedure for the treatment of anal fistula. This preliminary study was designed to investigate the safety and effectiveness of this new technique in the treatment of anal fistula. METHODS: Ten patients were treated with PDT. Intralesional 5-aminolevulinic acid (ALA) 2% was directly injected into the fistula. The internal and external orifices were closed. After an incubation period of 2 h, the fistula was irradiated using an optical fibre connected to a red laser (MULTIDIODE 630 PDT, INTERmedic, Spain) operating at 1 W/cm for 3 min (180 Joules)...
January 20, 2017: Techniques in Coloproctology
https://www.readbyqxmd.com/read/28108813/relationship-between-il-27-and-coronary-arterial-lesions-in-children-with-kawasaki-disease
#18
Feifei Si, Yao Wu, Fang Gao, Siqi Feng, Ruixi Liu, Qijian Yi
Kawasaki disease (KD) arises due to the disorder of the inflammation response and faulty immune regulation. Interleukin-27 (IL-27) is a novel cytokine with both pro-inflammatory and anti-inflammatory effects. This study investigated the relationship between serum levels of IL-27, Interleukin-17A (IL-17A), Interleukin-10 (IL-10), Interleukin-6 (IL-6), Interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α) and coronary artery lesions (CALs) in patients with KD. We obtained blood samples from 81 children with KD before intravenous immunoglobulin (IVIG) therapy...
January 20, 2017: Clinical and Experimental Medicine
https://www.readbyqxmd.com/read/28108432/living-on-the-edge-conservation-genetics-of-seven-thermophilous-plant-species-in-a-high-arctic-archipelago
#19
Siri Birkeland, Idunn Elisabeth Borgen Skjetne, Anne Krag Brysting, Reidar Elven, Inger Greve Alsos
Small, isolated, and/or peripheral populations are expected to harbour low levels of genetic variation and may therefore have reduced adaptability to environmental change, including climate warming. In the Arctic, global warming has already caused vegetation change across the region and is acting as a significant stressor on Arctic biodiversity. Many of the rare plants in the Arctic are relicts from early Holocene warm periods, but their ability to benefit from the current warming is dependent on the viability of their populations...
January 20, 2017: AoB Plants
https://www.readbyqxmd.com/read/28108271/comparative-effectiveness-of-busulfan-and-fludarabine-versus-fludarabine-and-400-cgy-total-body-irradiation-conditioning-regimens-for-aml-mds
#20
Moaath Mustafa Ali, Donna M Abounader, Lisa A Rybicki, Melissa A Yurch, Jamie Starn, Christina Ferraro, Victoria Winslow, Betty K Hamilton, Aaron T Gerds, Hien Liu, Robert Dean, Brian T Hill, Brad Pohlman, Steven Andresen, Rabi Hanna, Matt Kalaycio, Brian J Bolwell, Navneet S Majhail, Ronald Sobecks
Allogeneic hematopoietic cell transplantation (alloHCT) conditioning regimen intensity has varied for patients with acute myeloid leukemia (AML) and myelodysplastic syndrome (MDS). A comparative effectiveness analysis was performed to assess outcomes of busulfan and fludarabine (BuFlu) versus fludarabine and 400 cGy total body irradiation (FluTBI) conditioning. Thirty-three subjects received BuFlu and 38 had FluTBI. The BuFlu group received more red blood cell transfusions (P=0.02) and had a longer time to platelet recovery (P=0...
January 17, 2017: Biology of Blood and Marrow Transplantation
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