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https://www.readbyqxmd.com/read/29656462/kdm1a-regulated-the-osteo-dentinogenic-differentiation-process-of-the-stem-cells-of-the-apical-papilla-via-binding-with-plod2
#1
Lijun Wang, Haoqing Yang, Xiao Lin, Yangyang Cao, Peipei Gao, Ying Zheng, Zhipeng Fan
OBJECTIVES: Dental tissue-derived mesenchymal stem cells (MSCs)-mediated pulp-dentin regeneration is considered a potential approach for the regeneration of damaged teeth. Enhancing MSC-mediated pulp-dentin regeneration is based on an understanding of the molecular mechanisms underlying directed cell differentiation process. Histone demethylation enzyme, lysine demethylase 1A (KDM1A) can regulate the differentiation of some MSCs, but its role in dental tissue-derived MSCs is unclear. MATERIAL AND METHODS: We obtained SCAPs from immature teeth...
April 15, 2018: Cell Proliferation
https://www.readbyqxmd.com/read/29656368/fluorometric-aptamer-based-assay-for-ochratoxin-a-based-on-the-use-of-exonuclease-iii
#2
Renjie Liu, Hua Wu, Lei Lv, Xiaojiao Kang, Chengbi Cui, Jin Feng, Zhijun Guo
This study describes an aptamer based assay for the mycotoxin ochratoxin A (OTA). The method is based on the use of an OTA-specific aptamer, exonuclease (Exo) III, SYBR Gold as a fluorescent probe, and a complementary strand that specifically combines with the aptamer. In the presence of OTA, the aptamer and OTA hybridize, thereby resulting in the formation of ssDNA, which is not digested by Exo III. Intense fluorescence is observed after addition of SYBR Gold (best measured at excitation/emission wavelengths of 495/540 nm)...
April 14, 2018: Mikrochimica Acta
https://www.readbyqxmd.com/read/29656346/hip-fracture-patients-who-experience-a-greater-fluctuation-in-rdw-during-hospital-course-are-at-heightened-risk-for-all-cause-mortality-a-prospective-study-with-2-year-follow-up
#3
P Yin, H Lv, Y Li, Y Meng, L Zhang, L Zhang, P Tang
This study aims to detect whether there remains valuable prognostic information in fluctuation of red cell distribution width (RDW) in hip fracture patients. Results show that this readily available parameter may provide a more effective strategy for assessment of mortality risk, therefore providing a reference for clinical planning and decision-making. INTRODUCTION: Prognostic values have been found in the fluctuation of some hematologic parameters. The red cell distribution width (RDW) routinely reported with all complete blood cell counts (CBC) has proven to be associated with poor outcomes in various diseases...
April 14, 2018: Osteoporosis International
https://www.readbyqxmd.com/read/29656177/contribution-of-raman-and-surface-enhanced-raman-spectroscopy-sers-to-the-analysis-of-vehicle-headlights-dye-s-characterization
#4
Cyril Muehlethaler, Yin Pak Cheng, Syed K Islam, John R Lombardi
Although ubiquitous on accident scenes, the polymers from headlight optics are often neglected in hit-and-run cases, and their evidential value restrained to direct comparison once a corresponding vehicle is found. Multilayered automotive paint fragments are preferred for their access to corresponding databases (PDQ, EUCAP) to infer models and brands of cars. The potential of polymers headlights for providing forensic intelligence has never been exploited, principally due to the lack of diversity, of appropriate databases, and of case examples...
March 31, 2018: Forensic Science International
https://www.readbyqxmd.com/read/29656164/lignolytic-mushroom-lenzites-elegans-wdp2-laccase-production-characterization-and-bioremediation-of-synthetic-dyes
#5
Raj Kumar Pandey, Salil Tewari, Lakshmi Tewari
A mycoremedial study was undertaken for decolourization of synthetic dyes using wood rot fungal culture Lenzites elegans WDP2. The culture was isolated from decaying wood as fruiting body, and identified on the basis of 5.8S ITS rRNA gene sequence analysis. Qualitative plate screening of culture showed extracellular laccase and lignin peroxidase production, while only laccase enzyme was produced in higher amount (156.793 Uml-1 ) in minimal salt broth medium containing glucose and veratryl alcohol. Laccase activity was increased up to 189...
April 12, 2018: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/29656091/serial-reversal-learning-and-behavioural-flexibility-in-two-species-of-neotropical-parrots-diopsittaca-nobilis-and-pionites-melanocephala
#6
Jayden O van Horik, Nathan J Emery
Serial reversal learning of colour discriminations was assessed as an index of behavioural flexibility in two captive species of Neotropical parrots. Both species showed similar performances across serial reversals and no between species differences were observed. In a second task subjects' performances were assessed after they experienced either a low or high pre-reversal learning criterion. If reversal performances improve through processes of associative learning, a high pre-reversal criterion is expected to strengthen previously learned associations and hence impede post-reversal performances...
April 12, 2018: Behavioural Processes
https://www.readbyqxmd.com/read/29655791/overexpression-of-a-repressor-mdmyb15l-negatively-regulates-anthocyanin-and-cold-tolerance-in-red-fleshed-callus
#7
Haifeng Xu, Guanxian Yang, Jing Zhang, Yicheng Wang, Tianliang Zhang, Nan Wang, Shenghui Jiang, Zongying Zhang, Xuesen Chen
The cold-induced metabolic pathway and anthocyanin biosynthesis play important roles in plant growth. In this study, we identified a bHLH binding motif in the MdMYB15L protein using protein sequence analyses. Yeast two-hybrid and pull-down assays showed that MdMYB15L could interact with MdbHLH33. Overexpressing MdMYB15L in red-fleshed callus inhibited the expression of MdCBF2 and resulted in reduced cold tolerance but did not affect anthocyanin levels. Chip-PCR and EMSA analysis showed that MdMYB15L could bind the type II cis-acting element found in the MdCBF2 promoter...
April 12, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29655781/the-hyperopic-effect-of-narrow-band-long-wavelength-light-in-tree-shrews-increases-non-linearly-with-duration
#8
Alexander H Ward, Thomas T Norton, Carrie E Huisingh, Timothy J Gawne
During postnatal refractive development, an emmetropization mechanism uses refractive error to modulate the growth rate of the eye. Hyperopia (image focused behind the retina) produces what has been described as "GO" signaling that increases growth. Myopia (image focused in front of the retina) produces "STOP" signaling that slows growth. The interaction between GO and STOP conditions is non-linear; brief daily exposure to STOP counteracts long periods of GO. In young tree shrews, long-wavelength (red) light, presented 14 h per day, also appears to produce STOP signals...
April 12, 2018: Vision Research
https://www.readbyqxmd.com/read/29655754/green-and-fast-determination-of-the-alcoholic-content-of-wines-using-thermal-infrared-enthalpimetry
#9
Alessandra S Oliveira, Cristiano A Ballus, Cristiano R Menezes, Roger Wagner, José Neri G Paniz, Bruna Tischer, Adilson B Costa, Juliano S Barin
An innovative use of thermal infrared enthalpimetry (TIE) is proposed for the determination of alcoholic content of red and white wines. Notwithstanding the presence of ethanol in beverages, absolute ethanol was added directly to wines, and the temperature rise caused by the heat of dilution was monitored using an infrared camera. Analytical signals were obtained in only 10 s for four samples simultaneously, and a calibration curve was constructed with hydroalcoholic reference solutions. A linear calibration curve was obtained from 3...
August 30, 2018: Food Chemistry
https://www.readbyqxmd.com/read/29655748/-87-sr-86-sr-isotopes-in-grapes-of-different-cultivars-a-geochemical-tool-for-geographic-traceability-of-agriculture-products
#10
Ines Tescione, Sara Marchionni, Martina Casalini, Nadia Vignozzi, Massimo Mattei, Sandro Conticelli
87 Sr/86 Sr was determined on fresh red and white grapes, soils and rocks from three selected vineyards to verify the isotopic relationships between the fruit of the vine and geologic substrata of vineyards. 87 Sr/86 Sr were determined on sampled grapes of four different harvest years and different grape varieties, on bioavailable fraction of soils, on whole soils, and on bedrocks from the geo-pedological substratum of the vineyards. The vineyards chosen for the experimental works belong to an organic farming winery and thus cultivation procedures were strictly controlled...
August 30, 2018: Food Chemistry
https://www.readbyqxmd.com/read/29655700/effects-on-oral-fat-load-of-a-nutraceutical-combination-of-fermented-red-rice-sterol-esters-and-stanols-curcumin-and-olive-polyphenols-a-randomized-placebo-controlled-trial
#11
Giuseppe Derosa, Gabriele Catena, Riccardo Raddino, Giovanni Gaudio, Antonio Maggi, Angela D'Angelo, Pamela Maffioli
BACKGROUND: In literature, there are several studies about the effects of nutraceutical combinations at fasting, but data in post-prandial phase are lacking. PURPOSE: We planned a study to evaluate the efficacy and safety of a nutraceutical agent containing fermented red rice, phytosterols and olive polyphenols compared to placebo in a sample of Caucasian patients with low cardiovascular risk, both at fasting and after an oral fat load. STUDY DESIGN: Eighty patients were randomized to receive, as addition to diet and physical activity, a nutraceutical combination containing fermented red rice, sterol esters and stanols, curcumin, and olive polyphenols or placebo (control group), once a day...
March 15, 2018: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
https://www.readbyqxmd.com/read/29655424/simultaneous-elastase-hyaluronidase-and-collagenase-capillary-electrophoresis-based-assay-application-to-evaluate-the-bioactivity-of-the-red-alga-jania-rubens
#12
Syntia Fayad, Mona Tannoury, Philippe Morin, Reine Nehmé
There have been many efforts to search for affordable and efficient cosmetic ingredients from natural sources and to evaluate their bioactivities using eco-responsible tools. Hyaluronidase, elastase and collagenase are responsible for the degradation of the main components of the extracellular matrix, namely the hyaluronic acid, elastin and collagen, respectively. The aim of this work was to develop a single capillary electrophoresis method to monitor simultaneously the activities of these three enzymes, without reactant immobilization or radioactivity use...
August 22, 2018: Analytica Chimica Acta
https://www.readbyqxmd.com/read/29655377/schistosomicidal-antifibrotic-and-antioxidant-effects-of-cucurbita-pepo-l-seed-oil-and-praziquantel-combined-treatment-for-schistosoma-mansoni-infection-in-a-mouse-model
#13
E V N Beshay, A A Rady, A F Afifi, A H Mohamed
Despite the seriousness of schistosomiasis, its treatment depends only on praziquantel (PZQ), which has begun to lose its efficacy against the emergent Schistosoma mansoni-resistant strains. Therefore, the discovery of a novel schistosomicidal drug is an urgent priority. This study was designed to evaluate treatment with Cucurbita pepo L. (pumpkin) seed oil (PSO) alone and combined with PZQ against S. mansoni in experimentally infected mice. The study involved five groups: GI was the normal control; GII was the infected control; GIII was treated with an oral dose of PZQ of 500 mg/kg/day for two successive days, starting in the sixth week post infection; GIV was treated with an oral dose of PSO of 50 mg/kg/day for four weeks, starting in the fourth week post infection; and GV was treated with combined PSO-PZQ...
April 15, 2018: Journal of Helminthology
https://www.readbyqxmd.com/read/29653408/a-new-flavonoid-from-stellera-chamaejasme-l-stechamone-alleviated-2-4-dinitrochlorobenzene-induced-atopic-dermatitis-like-skin-lesions-in-a-murine-model
#14
Beom-Geun Jo, No-June Park, Jonghwan Jegal, Sangho Choi, Sang Woo Lee, Hang Jin, Su-Nam Kim, Min Hye Yang
Stellera chamaejasme L. (family Thymelaeaceae), also known as 'Langdu', has been traditionally used to treat of skin-related diseases, such as, psoriasis and skin ulcers. The aim of this study was to identify the biologically active component of S. chamaejasme and evaluate its preventive effects on IL-4 and mast cell degranulation in RBL-2H3 cells and on the development of atopic dermatitis (AD) in 2,4-dinitrochlorobenzene (DNCB)-treated SKH-1 hairless mice. A novel flavonoid, genkwanin 5-O-xylosyl(1 → 2)glucoside (named stechamone), and three known compounds (umbelliferone, luteolin, and luteolin-7-O-glucoside) were isolated from the aerial parts of S...
April 10, 2018: International Immunopharmacology
https://www.readbyqxmd.com/read/29653407/a-new-alzheimer-s-disease-cell-model-using-b-cells-to-induce-beta-amyloid-plaque-formation-and-increase-tnf-alpha-expression
#15
Mehrouz Dezfulian
Different cell models have been developed for the study of Alzheimer's disease (AD) pathways. The neuronal dysfunction and cell death mechanisms that are commonly found in this disease are due to the production of high levels of cytokines and the formation of amyloid plaques. In the cell model introduced in the present study, the production of these two important factors is induced by using B cells from an AD patient. The B cells of an Alzheimer's patient and a normal control were immortalized by using EBV (Epstein-Barr virus) to produce a lymphoblastoid cell line (LCL)...
April 10, 2018: International Immunopharmacology
https://www.readbyqxmd.com/read/29653392/electrolyzed-water-and-mild-thermal-processing-of-atlantic-salmon-salmo-salar-reduction-of-listeria-monocytogenes-and-changes-in-protein-structure
#16
Mahmoudreza Ovissipour, Setareh Ghorban Shiroodi, Barbara Rasco, Juming Tang, Shyam S Sablani
This study investigated the effect of different electrolyzed water (EO) solutions, including acidic electrolyzed water (AEW) and neutral electrolyzed water (NEW), alone and in combination with mild thermal processing (50, 55, 60, 65 °C) at different exposure times (2, 6, 10 min) on the reduction of Listeria monocytogenes on Atlantic salmon fillets. The effects of the EO water solutions on cell wall structures of L. monocytogenes and on the secondary structure of salmon protein were studied using Fourier Transform Infra-Red (FTIR) spectroscopy...
April 4, 2018: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/29653389/how-does-the-microbial-load-affect-the-quality-of-equine-cool-stored-semen
#17
E Varela, J Rey, E Plaza, P Muñoz de Propios, J M Ortiz-Rodríguez, M Álvarez, L Anel-López, L Anel, P De Paz, M C Gil, J M Morrell, C Ortega-Ferrusola
Contaminating bacteria present in stallion ejaculates may compromise sperm quality during storage. Different procedures have been used to reduce the load of microorganisms in semen and avoid bacterial growth during storage. The aims of this study were: 1) to evaluate different techniques to eliminate bacteria in semen 2) to study the relationship between total microflora load (TML) and ROS production; and 3) to determine if TML affects the functionality of cool-stored sperm. Ejaculates from 11 stallions were split and processed in 3 ways: A...
March 26, 2018: Theriogenology
https://www.readbyqxmd.com/read/29653176/hyperactive-piggybac-transposase-improves-transformation-efficiency-in-diverse-insect-species
#18
Kolja N Eckermann, Hassan M M Ahmed, Mohammad KaramiNejadRanjbar, Stefan Dippel, Christian E Ogaugwu, Peter Kitzmann, Musa D Isah, Ernst A Wimmer
Even in times of advanced site-specific genome editing tools, the improvement of DNA transposases is still on high demand in the field of transgenesis: especially in emerging model systems where evaluated integrase landing sites have not yet been created and more importantly in non-model organisms such as agricultural pests and disease vectors, in which reliable sequence information and genome annotations are still pending. In fact, random insertional mutagenesis is essential to identify new genomic locations that are not influenced by position effects and thus can serve as future stable transgene integration sites...
April 10, 2018: Insect Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/29653135/are-red-yellow-green-and-blue-perceptual-categories
#19
Christoph Witzel, Karl R Gegenfurtner
This study investigated categorical perception for unique hues in order to establish a relationship between color appearance, color discrimination, and low-level (second-stage) mechanisms. We tested whether pure red, yellow, green, and blue (unique hues) coincide with troughs, and their transitions (binary hues) with peaks of sensitivity in DKL-space. Results partially confirmed this idea: JNDs demarcated perceptual categories at the binary hues around green, blue and less clearly around yellow, when colors were isoluminant with the background and when accounting for the overall variation of sensitivity by fitting an ellipse...
April 10, 2018: Vision Research
https://www.readbyqxmd.com/read/29652918/a-genome-wide-association-study-of-red-blood-cell-fatty-acids-and-ratios-incorporating-dietary-covariates-framingham-heart-study-offspring-cohort
#20
Anya Kalsbeek, Jenna Veenstra, Jason Westra, Craig Disselkoen, Kristin Koch, Katelyn A McKenzie, Jacob O'Bott, Jason Vander Woude, Karen Fischer, Greg C Shearer, William S Harris, Nathan L Tintle
Recent analyses have suggested a strong heritable component to circulating fatty acid (FA) levels; however, only a limited number of genes have been identified which associate with FA levels. In order to expand upon a previous genome wide association study done on participants in the Framingham Heart Study Offspring Cohort and FA levels, we used data from 2,400 of these individuals for whom red blood cell FA profiles, dietary information and genotypes are available, and then conducted a genome-wide evaluation of potential genetic variants associated with 22 FAs and 15 FA ratios, after adjusting for relevant dietary covariates...
2018: PloS One
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