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G H Chen, H W Huang, Y Wang, H W Liu, L J Xu, X Ma, S L Xue, X F He, Y Wang, B Gu, C X Li, H Y Qiu, X W Tang, Z M Jin, M Miao, A N Sun, D P Wu
Objective: To study the specific killing effect of CD4 membrane protein targeted chimeric antigen receptor modified T (CAR-T) cell. Methods: The second generation CD4 targeted chimeric antigen receptor containing 4-1BB costimulation domain was insert into lentiviral vector through recombinant DNA technology. Lentivirus was prepared and packaged by 293T cells with four plasmids. Beads activated T cells were transduced with lentivirus and the transduction efficiency was checked with Protein L and flow cytometry...
February 14, 2018: Zhonghua Xue Ye Xue za Zhi, Zhonghua Xueyexue Zazhi
Ju-Hee Park, Young-Woo Kim, Yeon-Seong Jeong, Hee-Jae Suh, Hyang-Sook Chun, Gun-Young Lee, Ho-Soo Lim, Chan Lee
In this study, an improved analytical method for the detection of the colorant Brown FK in foods using high-performance liquid chromatography was developed. The method, which employed an RC-C18 column and diode array detection at 254 nm with sodium acetate solution and methanol as mobile phases, exhibited good linearity (R2 = 1.0), and its limits of detection and quantification were determined to be 0.06 and 0.19 μg/mL, respectively. The precision was found to be 0-1.2% and the accuracy was between 86.5% and 94...
March 17, 2018: Journal of Chromatographic Science
Kirila Stojnova, Vidka Divarova, Petya Racheva, Kristina Bozhinova, Vanya Lekova
The equilibria of the chelate formation and ion-association in the liquid-liquid extraction system Mo(VI)‒3,5-DNC‒INT‒H2O‒CHCl3 were studied by spectrophotometry. The optimum conditions for the chelate formation and extraction of the ion-associated complex formed between the anionic chelate of Mo(VI)‒3,5-dinitrocatechol (3,5-DNC) and the cation of 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride (INT) were established. The validity of Beer's law was checked and some analytical characteristics were calculated...
March 2018: Acta Chimica Slovenica
Mingxia Zhao, Guomei Wang, Yueqiang Leng, Humphrey Wanjugi, Pinggen Xi, Mike Grosz, Mohamed Mergoum, Shaobin Zhong
ND2710 is a hard red spring wheat line with a very high level of resistance to Fusarium head blight (FHB). It was selected from the progeny of a cross between ND2603 (an advanced breeding line derived from the Sumai 3/Wheaton cross) and Grandin (a spring wheat cultivar). The FHB resistance of ND2710 is presumably derived from Sumai 3 since the other parents Grandin and Wheaton are very susceptible to FHB. To identify and map the quantitative trait loci (QTL) for FHB resistance in ND2710, we developed a mapping population consisting of 233 recombinant inbred lines (RILs) from the cross between ND2710 and the spring wheat cultivar 'Bobwhite'...
March 21, 2018: Phytopathology
Raquel González, Clara Pons-Duran, Mireia Piqueras, John J Aponte, Feiko O Ter Kuile, Clara Menéndez
BACKGROUND: The World Health Organization recommends intermittent preventive treatment in pregnancy (IPTp) with sulfadoxine-pyrimethamine for malaria for all women who live in moderate to high malaria transmission areas in Africa. However, parasite resistance to sulfadoxine-pyrimethamine has been increasing steadily in some areas of the region. Moreover, HIV-infected women on cotrimoxazole prophylaxis cannot receive sulfadoxine-pyrimethamine because of potential drug interactions. Thus, there is an urgent need to identify alternative drugs for prevention of malaria in pregnancy...
March 21, 2018: Cochrane Database of Systematic Reviews
Guillermo Chiappe, Enrique Louis, J A Verges
Electronic polarizability of finite chains is accurately calculated from the total energy
 variation of the system produced by small but finite static electric fields applied
 along the chain direction. Normalized polarizability, that is, polarizability divided
 by chain length, diverges as the second power of length for metallic systems but
 approaches a constant value for insulating systems. This behaviour provides a very convenient
 way to characterize the wave--function malleability of finite systems as it avoids the
 need of attaching infinite contacts to the chain ends...
March 21, 2018: Journal of Physics. Condensed Matter: An Institute of Physics Journal
Matt Luther, Fergus Gardiner, Shane Lenson, David Caldicott, Ryan Harris, Ryan Sabet, Mark Malloy, Jo Perkins
Specific Event Identifiers a. Event type: Outdoor music festival. b. Event onset date: December 3, 2016. c. Location of event: Regatta Point, Commonwealth Park. d. Geographical coordinates: Canberra, Australian Capital Territory (ACT), Australia (-35.289002, 149.131957, 600m). e. Dates and times of observation in latitude, longitude, and elevation: December 3, 2016, 11:00-23:00. f. Response type: Event medical support. Abstract Introduction Young adult patrons are vulnerable to risk-taking behavior, including drug taking, at outdoor music festivals...
March 21, 2018: Prehospital and Disaster Medicine
Moses Kim, Jae Hyung Kim, Tae Hyung Lim, Beom Jin Cho
PURPOSE: To evaluate and compare visual acuity and reading speed for Korean language between a diffractive bifocal and trifocal intraocular lens (IOL) of the same material and haptic design. METHODS: We reviewed the medical records of the patients who had undergone bilateral cataract surgery with bifocal IOLs (AT LISA 801) on the both eyes (bifocal group) and trifocal IOLs (AT LISA tri 839 MP, trifocal group). The main outcome measures were the uncorrected distance, intermediate, and near visual acuity (uncorrected distance visual acuity [UCDVA], uncorrected intermediate visual acuity [UCIVA], and uncorrected near visual acuity [UCNVA]) and corrected distance, near, and distance-corrected intermediate visual acuity (corrected distance visual acuity [CDVA], corrected near visual acuity [CNVA], and distance-corrected intermediate visual acuity [DCIVA]) at last postoperative follow-up month...
March 19, 2018: Korean Journal of Ophthalmology: KJO
Giuseppe Cardellini, Tatiana Valada, Claire Cornillier, Estelle Vial, Marian Dragoi, Venceslas Goudiaby, Volker Mues, Bruno Lasserre, Arkadiusz Gruchala, Per Kristian Rørstad, Mathias Neumann, Miroslav Svoboda, Risto Sirgmets, Olli-Pekka Näsärö, Frits Mohren, Wouter M J Achten, Liesbet Vranken, Bart Muys
Life cycle assessment (LCA) has become a common methodology to analyze environmental impacts of forestry systems. Although LCA has been widely applied to forestry since the 90s, the LCAs are still often based on generic Life Cycle Inventory (LCI). With the purpose of improving LCA practices in the forestry sector, we developed a European Life Cycle Inventory of Forestry Operations (EFO-LCI) and analyzed the available information to check if within the European forestry sector national differences really exist...
March 20, 2018: Environmental Management
Shuai Kang, Mingwei Shang, Matthew A Spence, Michael Andrew, Shuangyi Liu, Junjie Niu
A methodology to screen additives in lead-acid batteries is critical. We developed a three-electrode system that can rapidly check the dynamic charge acceptance (DCA) and hydrogen evolution of electrodes. The electrode with 2% MXene and 0.2% carbon black shows a better DCA, which indicates its great potential in the start-stop technology.
March 21, 2018: Chemical Communications: Chem Comm
Bruno Gonfiotti, Sandro Paci
During the last 40 years, many studies have been carried out to investigate the different phenomena occurring during a Severe Accident (SA) in a Nuclear Power Plant (NPP). Such efforts have been supported by the execution of different experimental campaigns, and the integral Phébus FP tests were probably some of the most important experiments in this field. In these tests, the degradation of a Pressurized Water Reactor (PWR) fuel bundle was investigated employing different control rod materials and burn-up levels in strongly or weakly oxidizing conditions...
March 2018: Heliyon
Gouri Chaubal, Prasad Sarkale, Pravin Kore, Pragya Yadav
Background: Kyasanur Forest Disease (KFD), a tick borne flavivirus, which was earlier endemic to Karnataka state, India, has been confirmed and detected from neighboring states of Tamil Nadu, Maharashtra, Goa and Kerala states in India. Increased human and vector surveillance therefore becomes essential for the identification of KFD affected regions and control of further spread of the disease. Currently, available KFD detection assays include realtime RT-PCR and nested RT-PCR assays...
February 2018: Heliyon
Shahram Bamdad, Anis Shamsi Kooshki, Masoud Yasemi
Background: Pterygium is a common degenerative eye disease. Despite various surgical methods to treat pterygium, recurrence is the main complication. The main issue is finding a surgical method with the lowest recurrence. Objective: to compare the complications, recurrence rate and the cosmetic effects of two surgical techniques, namely conjunctival rotation autograft (CRA) and conjunctival autograft (CA), in treating pterygium. Methods: This randomized clinical trial was conducted at Khalili Hospital in Shiraz, Iran, from January to August 2015...
December 2017: Electronic Physician
Shiming Cheng, Deanna Tollefson, Guangxue He, Yuan Li, Hui Guo, Shua Chai, Fangfang Gao, Fei Gao, Guoxin Han, Liping Ren, Yulin Ren, Jianbo Li, Lixia Wang, Jay K Varma, Dongmei Hu, Haiying Fan, Fei Zhao, Emily Bloss, Yu Wang, Carol Y Rao
Background: Health care workers are at high risk for tuberculosis (TB). China, a high burden TB country, has no policy on medical surveillance for TB among healthcare workers. In this paper, we evaluate whether China's national TB diagnostic guidelines could be used as a framework to screen healthcare workers for pulmonary TB disease in a clinical setting in China. Methods: Between April-August 2010, healthcare workers from 28 facilities in Inner Mongolia Autonomous Region, China were eligible for TB screening, comprised of symptom check, chest X-ray and tuberculin skin testing...
2018: Journal of Occupational Medicine and Toxicology
Jianyun Lu, Miaomiao Li, Lihua Gao, Yizhi Pan, Yaping Xiang, Jinhua Huang, Xianzhen Jiang
To determine initial concentrations of ozonated water under different temperatures, attenuation rules of ozonated water under the room temperature (25 ℃), and to inspect the effects of ozonated water under different concentrations on common microorganisms.
 Methods: The online test method and the plate cultivation method were employed to check the concentrations and killing rates on common microorganisms of ozonated water produced by HZ-2601 B Ozone Water Generating Instrument.
 Results: The initial concentrations of ozonated water at 20, 25, 30, 35, and 40 ℃ were 4...
February 28, 2018: Zhong Nan da Xue Xue Bao. Yi Xue Ban, Journal of Central South University. Medical Sciences
Jennifer L Walton, Diane K Dunn, Nhi Y Haines, Ilana Heisler, Michael T Bigham, Teresa A Volsko
BACKGROUND: The American Academy of Pediatrics Section on Transport recommends the use of portable ventilators during the transport of patients with advanced airways. We sought to identify knowledge gaps and evaluate the effectiveness of a transport ventilator competency boot camp. METHODS: Electronic health records of children requiring ventilatory support during air and ground interfacility transport from January 1 through December 31, 2015, were reviewed to determine when manual ventilation was used in lieu of a portable ventilator, and simulations were constructed from commonly occurring scenarios...
March 20, 2018: Respiratory Care
Sehar Qudsia, Siva B Merugu, Hitesh B Mangukiya, Negi Hema, Zhenghua Wu, Dawei Li
Antibody display libraries have become a popular technique to screen monoclonal antibodies for therapeutic purposes. An important aspect of display technology is to generate an optimization library by changing antibody affinity to antigen through mutagenesis and screening the high affinity antibody. In this study, we report a novel lentivirus display based optimization library antibody in which Agtuzumab scFv is displayed on cell membrane of HEK-293T cells. To generate an optimization library, hotspot mutagenesis was performed to achieve diverse antibody library...
March 17, 2018: Biochemical and Biophysical Research Communications
Erin J Sanchez, Lee F Gabler, Ann B Good, James R Funk, Jeff R Crandall, Matthew B Panzer
BACKGROUND: Head kinematics generated by laboratory reconstructions of professional football helmet impacts have been applied to computational models to study the biomechanics of concussion. Since the original publication of this data, techniques for evaluating accelerometer consistency and error correction have been developed. This study applies these techniques to the original reconstruction data and reanalyzes the results given the current state of concussion biomechanics. METHODS: Consistency checks were applied to the sensor data collected in the head of each test dummy...
March 14, 2018: Clinical Biomechanics
Panagiotis Azmanis, Antonio di Somma, Lucia Pappalardo, Christu-Das Silvanose, Berit Bangoura
Cryptosporidiosis in raptors and falcons is well-known to be caused by Cryptosporidium baileyi and associated mainly with respiratory pathology. This report presents the diagnosis of an atypical cryptosporidiosis event caused by Cryptosporidium parvum, that to the authors' knowledge, is a case observed for the first time in falcons. Two falcons (Gyrfalcon x Peregrine hybrids) were presented for annual check without any clinical signs. Hematology, biochemistry, fecal and crop parasitology, radiographic and endoscopic examinations were performed...
March 15, 2018: Veterinary Parasitology
Abhinav Saurabh, Sushmita Chakraborty, Prabin Kumar, Anant Mohan, Anuj K Bhatnagar, Narayan Rishi, Dipendra Kumar Mitra
Human Leukocyte Antigen-G (HLA-G), a non-classical, class Ib molecule, has been shown to mediate immunoregulatory functions by inducing apoptosis, inhibits cytotoxicity and differentiation by modulating cytokine secretion. Due to its immune-suppressive function, it facilitates tolerance in feto-maternal interface and transplantation. In contrary, it favours immune evasion of microbes and tumors by inhibiting immune and inflammatory responses. In Tuberculosis (TB), we previously reported differential expression of HLA-G and its receptor Ig-like transcript -2 (ILT-2) in disseminated vs...
March 2018: Tuberculosis
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