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https://www.readbyqxmd.com/read/28092829/enhancing-4-propylheptane-dissociation-with-nickel-nanocluster-based-on-molecular-dynamics-simulations
#1
Margarita G Ilyina, Edward M Khamitov, Rail N Galiakhmetov, Ildar A Mustafin, Akhat G Mustafin
In the present work, a 0.4nm nickel cluster has been theoretically studied. Its equilibrium structural parameters have been calculated by the DFT method based on the PBEH1PBE hybrid functional and split-valence basis set Lanl2DZ including effective core potentials. We have systematically considered diverse spin states of this cluster and find out its ground state. The relative stability of these states depends on the HOMO-LUMO gap. The interaction of the Ni6 with 4-propylheptane С10Н22 has been studied to simulate the process of catalytic cracking of hydrocarbons...
January 3, 2017: Journal of Molecular Graphics & Modelling
https://www.readbyqxmd.com/read/28092265/the-fibronectin-synergy-site-re-enforces-cell-adhesion-and-mediates-a-crosstalk-between-integrin-classes
#2
Maria Benito-Jardón, Sarah Klapproth, Irene Gimeno-LLuch, Tobias Petzold, Mitasha Bharadwaj, Daniel J Müller, Gabriele Zuchtriegel, Christoph A Reichel, Mercedes Costell
Fibronectin (FN), a major extracellular matrix component, enables integrin-mediated cell adhesion via binding of α5β1, αIIbβ3 and αv-class integrins to an RGD-motif. An additional linkage for α5 and αIIb is the synergy site located in close proximity to the RGD motif. We report that mice with a dysfunctional FN-synergy motif (FNsyn/syn) suffer from surprisingly mild platelet adhesion and bleeding defects due to delayed thrombus formation after vessel injury. Additional loss of β3 integrins dramatically aggravates the bleedings and severely compromises smooth muscle cell coverage of the vasculature leading to embryonic lethality...
January 16, 2017: ELife
https://www.readbyqxmd.com/read/28088078/genetically-tailored-magnetosomes-used-as-mri-probe-for-molecular-imaging-of-brain-tumor
#3
M Boucher, F Geffroy, S Prévéral, L Bellanger, E Selingue, G Adryanczyk-Perrier, M Péan, C T Lefèvre, D Pignol, N Ginet, S Mériaux
We investigate here the potential of single step production of genetically engineered magnetosomes, bacterial biogenic iron-oxide nanoparticles embedded in a lipid vesicle, as a new tailorable magnetic resonance molecular imaging probe. We demonstrate in vitro the specific binding and the significant internalization into U87 cells of magnetosomes decorated with RGD peptide. After injection at the tail vein of glioblastoma-bearing mice, we evidence in the first 2 h the rapid accumulation of both unlabeled and functionalized magnetosomes inside the tumor by Enhanced Permeability and Retention effects...
December 19, 2016: Biomaterials
https://www.readbyqxmd.com/read/28086938/alpha-enolase-eno1-controls-alpha-v-beta-3-integrin-expression-and-regulates-pancreatic-cancer-adhesion-invasion-and-metastasis
#4
Moitza Principe, Simone Borgoni, Mariafrancesca Cascione, Michelle Samuel Chattaragada, Sammy Ferri-Borgogno, Michela Capello, Sara Bulfamante, Jennifer Chapelle, Francesca Di Modugno, Paola Defilippi, Paola Nisticò, Paola Cappello, Chiara Riganti, Stefano Leporatti, Francesco Novelli
BACKGROUND: We have previously shown that in pancreatic ductal adenocarcinoma (PDA) cells, the glycolytic enzyme alpha-enolase (ENO1) also acts as a plasminogen receptor and promotes invasion and metastasis formation. Moreover, ENO1 silencing in PDA cells induces oxidative stress, senescence and profoundly modifies PDA cell metabolism. Although anti-ENO1 antibody inhibits PDA cell migration and invasion, little is known about the role of ENO1 in regulating cell-cell and cell-matrix contacts...
January 13, 2017: Journal of Hematology & Oncology
https://www.readbyqxmd.com/read/28075550/vascularization-in-engineered-tissue-construct-by-assembly-of-cellular-patterned-micro-modules-and-degradable-microspheres
#5
Meiling Zhong, Dan Wei, You Yang, Jing Sun, Xuening Chen, Likun Guo, Qingrong Wei, Yizao Wan, Hongsong Fan, Xingdong Zhang
Tissue engineering aims to generate functional tissue constructs in which proper ECM for cell survival and establishment of vascular network are necessary. A modular approach via the assembly of modules mimicking the complex tissues' micro-architectural features and establishing vascular network represents a promising strategy for fabricating larger and more complex tissue constructs. Herein, as a model for this modular tissue engineering, engineered bone-like constructs were developed by self-assembly of osteon-like modules and fast degradable gelatin microspheres...
January 11, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28074920/a-comprehensive-evaluation-of-the-activity-and-selectivity-profile-of-ligands-for-rgd-binding-integrins
#6
Tobias G Kapp, Florian Rechenmacher, Stefanie Neubauer, Oleg V Maltsev, Elisabetta A Cavalcanti-Adam, Revital Zarka, Ute Reuning, Johannes Notni, Hans-Jürgen Wester, Carlos Mas-Moruno, Joachim Spatz, Benjamin Geiger, Horst Kessler
Integrins, a diverse class of heterodimeric cell surface receptors, are key regulators of cell structure and behaviour, affecting cell morphology, proliferation, survival and differentiation. Consequently, mutations in specific integrins, or their deregulated expression, are associated with a variety of diseases. In the last decades, many integrin-specific ligands have been developed and used for modulation of integrin function in medical as well as biophysical studies. The IC50-values reported for these ligands strongly vary and are measured using different cell-based and cell-free systems...
January 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28071891/dual-enzymatic-reaction-assisted-gemcitabine-delivery-systems-for-programmed-pancreatic-cancer-therapy
#7
Haijie Han, Daniel Valdeperez, Qiao Jin, Bin Yang, Zuhong Li, Yulian Wu, Beatriz Pelaz, Wolfgang J J Parak, Jian Ji
Dual enzymatic reactions were introduced to fabricate programmed gemcitabine (GEM) nanovectors for targeted pancreatic cancer therapy. Dual-enzyme sensitive GEM nanovectors were prepared by conjugation of matrix metalloproteinase-9 (MMP-9) detachable poly(ethylene glycol) (PEG), cathepsin B cleavable GEM, and targeting ligand CycloRGD to CdSe/ZnS quantum dots (QDs). The GEM nanovectors decorated with PEG corona could avoid nonspecific interactions and exhibit prolonged blood circulation time. After GEM nanovectors were accumulated in tumor tissue by the enhanced permeability and retention (EPR) effect, the PEG corona can be removed by overexpressed MMP-9 in tumor tissue and RGD would be exposed, which was capable of facilitating cellular internalization...
January 10, 2017: ACS Nano
https://www.readbyqxmd.com/read/28069495/surface-modification-of-copolymerized-films-from-three-armed-biodegradable-macromers-an-analytical-platform-for-modified-tissue-engineering-scaffolds
#8
Benno M Müller, Rudi Loth, Peter-Georg Hoffmeister, Friederike Zühl, Liv Kalbitzer, Michael C Hacker, Michaela Schulz-Siegmund
: The concept of macromers allows for a broad adjustment of biomaterial properties by macromer chemistry or copolymerization. Copolymerization strategies can also be used to introduce reactive sites for subsequent surface modification. Control over surface features enables adjustment of cellular reactions with regard to site and object of implantation. We designed macromer-derived polymer films which function as non-implantable analytical substrates for the investigation of surface properties of equally composed scaffolds for bone tissue engineering...
January 6, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28068609/dual-peptide-presenting-hydrogels-for-controlling-the-phenotype-of-pc12-cells
#9
Jae Won Lee, Kuen Yong Lee
Controlling the cell-matrix interaction is a critical factor in the design and fabrication of tissue engineering scaffolds. A particular peptide sequence, Arg-Gly-Asp (RGD peptide), is often used as an adhesion ligand in the engineering of different types of tissues. While in some cases this has been adequate, the use of multiple ligands may be required for the successful engineering of some tissue types. We hypothesized that hydrogels presenting both the RGD peptide and the YIGSR peptide (Tyr-Ile-Gly-Ser-Arg) could successfully regulate the phenotype of PC12 cells, thereby providing a new platform for effective tissue engineering applications...
January 3, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28068367/cell-surface-integrins-and-car-are-both-essential-for-adenovirus-type-5-transduction-of-canine-cells-of-lymphocytic-origin
#10
Payal Agarwal, Elizabeth A Gammon, Abdul Mohin Sajib, Maninder Sandey, Bruce F Smith
Adenoviruses are the most widely used vectors in cancer gene therapy. Adenoviruses vectors are well characterized and are easily manipulated. Adenovirus serotype 5 (Ad5) is the most commonly used human serotype. Ad5 internalization into host cells is a combined effect of binding of Ad5 fiber knob with the coxsackie virus and adenovirus receptor (CAR) and binding of RGD motifs in viral penton to cell surface integrins (αvβ3, αvβ5). Ad5's wide range of host-cell transduction and lack of integration into the host genome have made it an excellent choice for cancer therapeutics...
2017: PloS One
https://www.readbyqxmd.com/read/28065871/targeted-glioma-chemotherapy-by-cyclic-rgd-peptide-functionalized-reversibly-core-crosslinked-multifunctional-poly-ethylene-glycol-b-poly-%C3%AE%C2%B5-caprolactone-micelles
#11
Ya Fang, Yu Jiang, Yan Zou, Fenghua Meng, Jian Zhang, Chao Deng, Huanli Sun, Zhiyuan Zhong
: Cyclic RGD peptide-functionalized reversibly core-crosslinked biodegradable poly(ethylene glycol)-b-poly(ε-caprolactone) (PEG-PCL) micelles (cRGD-RCCMs) were designed and developed for highly potent and targeted glioma chemotherapy. To achieve crosslinkable core, dithiolane-functionalized trimethylene carbonate (DTC) was incorporated into PCL block. Interestingly, cRGD-RCCMs displayed a high doxorubicin (DOX) loading content of ∼18 wt.%, small hydrodynamic size of ∼50 nm, and excellent colloidal stability with minimum drug leakage under physiological conditions whilst fast DOX release under cytoplasmic-mimicking reductive environments...
January 5, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28050608/an-in-situ-poly-carboxybetaine-hydrogel-for-tissue-engineering-applications
#12
Hsiu-Wen Chien, Jiashing Yu, Shing Tak Li, Hsin-Yu Chen, Wei-Bor Tsai
Hydrogels provide three-dimensional (3D) frames with tissue-like elasticity and high water content for tissue scaffolds. Previously, we reported the design and synthesis protocol of a biodegradable poly(carboxybetaine) poly(CB) hydrogel with a zwitterionic carboxybetaine methacrylate (CBMA) monomer and a disulfide-containing crosslinker via free radical polymerization. We also demonstrated that cells could be successfully encapsulated in the hydrogels without compromising cytoviability. In this study, we evaluated the cytoviability of three commonly used zwitterionic monomers (CBMA, 2-methacryloyloxyethyl phosphorylcholine (MPC) and sulfobetaine methacrylate (SBMA)) and the suitability of being utilized as precursor materials for in situ gel forming implants...
January 4, 2017: Biomaterials Science
https://www.readbyqxmd.com/read/28047291/su-f-t-175-absorbed-dose-measurement-using-radiophotoluminescent-glass-dosimeter-in-therapeutic-proton-beam
#13
W Chang, Y Koba, T Katayose, M Hariu, K Yasui, H Saitoh
PURPOSE: To measure the absorbed dose to water Dw in therapeutic proton beam with radiophotoluminescent glass dosimeter (RGD), a methodology was proposed. In this methodology, the correction factor for the LET dependence of radiophotoluminescent (RPL) efficiency and the variation of mass stopping power ratio of water to RGD (SPRw, RGD) were adopted. The feasibility of proposed method was evaluated in this report. METHODS: The calibration coefficient in terms of Dw for RGDs (GD-302M, Asahi Techno Glass) was obtained using 60Co beam...
June 2016: Medical Physics
https://www.readbyqxmd.com/read/28044100/photocrosslinkable-chitosan-hydrogels-functionalized-with-the-rgd-peptide-and-phosphoserine-to-enhance-osteogenesis
#14
Soyon Kim, Zhong-Kai Cui, Jiabing Fan, Armita Fartash, Tara L Aghaloo, Min Lee
Hydrogels derived from naturally occurring polymers are attractive matrix for tissue engineering. Here, we report a biofunctional hydrogel for specific use in bone regeneration by introducing Arg-Gly-Asp (RGD)-containing cell adhesive motifs and phosphorylated serine residues, which are prevalent in native bone extracellular matrix and known to promote osteogenesis by enhancing cell-matrix interactions and hydroxyapatite nucleation, into photopolymerizable methacrylated glycol chitosan (MeGC). Incorporation of phosphoserine into MeGC hydrogels increased the ability of the hydrogels to nucleate mineral on their surfaces...
August 21, 2016: Journal of Materials Chemistry. B, Materials for Biology and Medicine
https://www.readbyqxmd.com/read/28043010/integrin-%C3%AE-v%C3%AE-3-promotes-infection-by-japanese-encephalitis-virus
#15
Wenchun Fan, Ping Qian, Dandan Wang, Xianwei Zhi, Yanming Wei, Huanchun Chen, Xiangmin Li
Japanese encephalitis virus (JEV) is a mosquito-borne flavivirus that is one of the major causes of viral encephalitis diseases worldwide. The JEV envelope protein facilitates viral entry, and its domain III contains an Arg-Gly-Asp (RGD) motif, that may modulate JEV entry through the RGD-binding integrin. In this study, the roles of integrin αv and β3 on the infection of JEV were evaluated. Reduced expression of integrin αv/β3 by special shRNA confers 2 to 4-fold inhibition of JEV replication in BHK-21 cells...
December 28, 2016: Research in Veterinary Science
https://www.readbyqxmd.com/read/28038913/rlj-rgd3-induces-apoptosis-via-the-mitochondrial-dependent-pathway-and-inhibits-adhesion-migration-and-invasion-of-human-heya8-cells-via-fak-pathway
#16
Qi Jiang, Qingwei Li, Jianmei Han, Meng Gou, Yuanyuan Zheng, Bowen Li, Rong Xiao, Jihong Wang
Ovarian carcinoma is a tumor derived from ovary, which brings relatively higher mortality rate among the fatal gynecological cancers. Recently, lots of studies have concentrated on the anti-tumor effects of Arg-Gly-Asp (RGD) motif containing peptides due to their integrin binding properties. In order to meet the criterion of genetic engineering drugs, a recombinant RGD toxin protein (rLj-RGD3) without a His-tag was cloned from the buccal glands of Lampetra japonica in the present study. After endotoxin removal, the His-tag removed rLj-RGD3 was shown to inhibit the proliferation of HeyA8 cells...
December 27, 2016: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28025149/substrate-stiffness-promotes-latent-tgf-%C3%AE-1-activation-in-hepatocellular-carcinoma
#17
Mingshu Pang, Yao Teng, Jianyong Huang, Yuan Yuan, Feng Lin, Chunyang Xiong
Hepatocellular carcinoma (HCC) was usually coupled with increased stiffness of the extracellular matrix (ECM) and elevated level of transforming growth factor-β1 (TGF-β1). However, the mechanism by which substrate rigidity modulated TGF-β1 signaling transduction remained unknown. This paper investigated the molecular mechanism of how matrix stiffness regulating TGF-β1 signaling in HCC cells. By means of stiffness tunable collagen I-coated polyacrylamide (PA) gels, we found that the expressions of β1 integrin, p-FAK (Y397) and p-Smad2 upregulated on stiffer gels as well as the content of TGF-β1 in culture media of HCC cells, which were inhibited by RGD blocking peptides, Y-27632 (ROCK inhibitor) or Blebbistatin (myosin II inhibitor)...
December 24, 2016: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28025144/bio-active-molecules-modified-surfaces-enhanced-mesenchymal-stem-cell-adhesion-and-proliferation
#18
Rezvan Mobasseri, Lingling Tian, Masoud Soleimani, Seeram Ramakrishna, Hossein Naderi-Manesh
Surface modification of the substrate as a component of in vitro cell culture and tissue engineering, using bio-active molecules including extracellular matrix (ECM) proteins or peptides derived ECM proteins can modulate the surface properties and thereby induce the desired signaling pathways in cells. The aim of this study was to evaluate the behavior of human bone marrow mesenchymal stem cells (hBM-MSCs) on glass substrates modified with fibronectin (Fn), collagen (Coll), RGD peptides (RGD) and designed peptide (R-pept) as bio-active molecules...
December 23, 2016: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28024192/next-generation-ultrasound-platforms-for-theranostics
#19
Letizia Oddo, Barbara Cerroni, Fabio Domenici, Angelico Bedini, Federico Bordi, Ester Chiessi, Stefan Gerbes, Gaio Paradossi
Microbubbles are a well-established contrast agent which improves diagnostic ultrasound imaging. During the last decade research has focused on expanding their use to include molecular imaging, targeted therapy and imaging modalities other than ultrasound. However, bioadhesion of targeted microbubbles under physiological flow conditions is still difficult to achieve, the main challenge being connected to the poor stability of lipid microbubbles in the body's circulation system. In this article, we investigate the use of polymeric microbubbles based on a poly (vinyl alcohol) shell as an alternative to lipid microbubbles...
December 18, 2016: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/28009982/precise-integrin-targeting-near-infrared-imaging-guided-surgical-method-increases-surgical-qualification-of-peritoneal-carcinomatosis-from-gastric-cancer-in-mice
#20
Haidong Cheng, Chongwei Chi, Wenting Shang, Sha Rengaowa, Jianxin Cui, Jinzuo Ye, Shixin Jiang, Yamin Mao, Caoting Zeng, Huiping Huo, Lin Chen, Jie Tian
Peritoneal carcinomatosis from gastric cancer represents a common recurrent gastric cancer that seriously affects the survival, prognosis, and quality of life of patients at its advanced stage. In recent years, complete cytoreduction surgery in combination with hyperthermic intraperitoneal chemotherapy has been demonstrated to improve the survival and prognosis of patients with malignant tumors including peritoneal carcinomatosis from gastric cancer. Establishing viable methods of accurately assessing the tumor burden in patients with peritoneal carcinoma and correctly selecting suitable patients in order to improve cytoreduction surgical outcomes and reduce the risk of postoperative complications has become a challenge in the field of peritoneal carcinoma research...
December 21, 2016: Oncotarget
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