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Ting Wang, Yi Hou, Bo Bu, Wenxin Wang, Tiancong Ma, Chunyan Liu, Lan Lin, Lin Ma, Xin Lou, Mingyuan Gao
Ischemic stroke is one of the major leading causes for long-term disability and mortality. Collateral vessels provide an alternative pathway to protect the brain against ischemic injury after arterial occlusion. Aiming at visualizing the collaterals occurring during acute ischemic stroke, an integrin αv β3 -specific Fe3 O4 -Arg-Gly-Asp (RGD) nanoprobe is prepared for magnetic resonance imaging (MRI) of the collaterals. Rat models are constructed by occluding the middle cerebral artery for imaging studies of cerebral ischemia and ischemia-reperfusion on 7...
April 17, 2018: Small
Zhenli Li, Han Zhang, Jun Han, Yu Chen, Han Lin, Tian Yang
Hepatocellular carcinoma (HCC) is one of the most common and deadly gastrointestinal malignancies. Given its insensitivity to traditional systematic chemotherapy, new therapeutic strategies for efficient HCCs treatment are urgently needed. Here, the development of a novel 2D MXene-based composite nanoplatform for highly efficient and synergistic chemotherapy and photothermal hyperthermia against HCC is reported. A surface-nanopore engineering strategy is developed for the MXenes' surface functionalization, which achieves the uniform coating of a thin mesoporous-silica layer onto the surface of 2D Ti3 C2 MXene (Ti3 C2 @mMSNs)...
April 16, 2018: Advanced Materials
Akanksha Jain, Sudipta Chakraborty, H D Sarma, Ashutosh Dash
Purpose: The present paper reports a systematic study on the effect of bifunctional chelators (BFC) namely, NOTA, DOTA, and DTPA, on the radiochemical formulation, in vitro stability, and in vivo biological properties of 68 Ga-labeled RGD peptide derivatives. Methods: The three RGD conjugates namely, NOTA-Bn-E-[c(RGDfk)]2 , DOTA-Bn-E-[c(RGDfk)]2 , and DTPA-Bn-E-[c(RGDfk)]2 were radiolabeled with 68 Ga and the radiolabeling was optimized with respect to the ligand amount, radiolabeling time, and temperature...
April 2018: Nuclear Medicine and Molecular Imaging
Filomena A Carvalho, Ana Filipa Guedes, Cedric Duval, Fraser L Macrae, Luke Swithenbank, David H Farrell, Robert As Ariëns, Nuno C Santos
Background: Erythrocyte aggregation, a cardiovascular risk factor, is increased by high plasma fibrinogen levels. Here, the effect of different fibrinogen mutations on binding to its human erythrocyte receptor was assessed in order to identify the interaction sites. Methods: Three fibrinogen variants were tested, specifically mutated in their putative integrin recognition sites on the Aα chain (mutants D97E, D574E and D97E/D574E) and compared with wild-type fibrinogen...
2018: International Journal of Nanomedicine
Francesco Saverio Di Leva, Stefano Tomassi, Salvatore Di Maro, Florian Reichart, Johannes Notni, Abha Dangi, Udaya Kiran Marelli, Diego Brancaccio, Francesco Merlino, Hans-Jürgen Wester, Ettore Novellino, Horst Kessler, Luciana Marinelli
The RGD-recognizing αvβ6 integrin has only recently emerged as a major target for cancer diagnosis and therapy. Thus, the development of selective, low molecular weight ligands of this receptor is still of great demand. Here, a metadynamics-driven design strategy allowed us to successfully convert a helical nonapeptide into a cyclic pentapeptide (6) showing remarkable potency and αvβ6 specificity. NMR and docking studies elucidated the reasons for the high affinity and selectivity of this compound, setting the ground for the rational design of new αvβ6-specific small sized peptides or even peptidomimetics...
April 16, 2018: Angewandte Chemie
Yang Huang, Jinsong Kong, Xiaokang Gong, Xin Zheng, Haibao Wang, Jianwei Ruan
<b>Objective:</b> To analysis the biomechanical and biocompatible properties of calcium phosphate cement (CPC) enhanced by chitosan short nanofibers(CSNF) and Arg-Gly-Asp (RGD). <b>Methods:</b> Chitosan nanofibers were prepared by electrospinning, and cut into short fibers by high speed dispersion. CPC with calcium phosphorus ratio of 1.5:1 was prepared by Biocement D method. The composition and structure of CPC, CSNF, RGD modified CSNF (CSNF-RGD), CSNF enhanced CPC (CPC-CSNF), RGD modified CPC-CSNF (CPC-CSNF-RGD) were observed by infrared spectrum, X-ray diffraction (XRD) and scan electron microscopy (SEM)...
May 25, 2017: Zhejiang da Xue Xue Bao. Yi Xue Ban, Journal of Zhejiang University. Medical Sciences
Wei Li, Danyang Sun, Ning Li, Yiming Shen, Yiming Hu, Jian Tan
Objective To evaluate the effectiveness of two kinds of Arg-Gly-Asp (RGD)-targeted 131 I-containing nanoliposomes for the treatment of cervical cancer in vitro and in vivo. Methods The nanoparticle liposomes designated RGD-131 I-tyrosine peptide chain (TPC)-L and 131 I-RGD-L were prepared. The emulsion solvent evaporation method was used to encapsulate the polypeptide into liposomes. The quantity of entrapped polypeptide was measured using UV spectrophotometry. The labeling rates, radiochemical purities, and total radioactivities were measured using paper chromatography...
January 1, 2018: Journal of International Medical Research
Yuuki Inoue, Yuya Onodera, Kazuhiko Ishihara
Dynamic changes in the properties of adsorbed protein layers at material surfaces make it difficult to analyze a cell adhesion behavior. Adhesion is affected by the ligand molecules in the adsorbed protein layers on the material's surface. This study aimed to quantitatively analyze the initial cell adhesion onto a polymeric surface modified with immobilized cell adhesion molecules with a well-defined structure. Peptides containing an arginine-glycine-aspartic acid (RGD) sequence were introduced at almost all the termini of the grafted poly(2-methacryloyloxyethyl phosphorylcholine) (poly(MPC)) chains using a click reaction at a highly protein-resistant poly(MPC) brush layer...
April 13, 2018: ACS Applied Materials & Interfaces
Daphne Lobeek, Gerben M Franssen, Michelle T Ma, Hans-Jürgen Wester, Clemens Decristoforo, Wim J G Oyen, Otto C Boerman, Samantha Y A Terry, Mark Rijpkema
Rationale: αv β3 integrins play an important role in angiogenesis and cell migration of cancers. They are highly expressed on activated endothelial cells of newly formed blood vessels. Here, we compare the targeting characteristics of four68 Ga-labeled multimeric cyclic arginine-glycine-aspartate RGD-based tracers in an αv β3 integrin-expressing tumor model and in a tumor model where αv β3 integrin is expressed solely on the neovasculature. Methods: Female BALB/c nude mice were subcutaneously injected with SK-RC-52 (αv β3 integrin-positive) or FaDu (αv β3 integrin-negative) tumor cells...
April 6, 2018: Journal of Nuclear Medicine: Official Publication, Society of Nuclear Medicine
Chara Simitzi, Pascal Harimech, Syrago Spanou, Christina Lanara, Amelie Heuer-Jungemann, Aleka Manousaki, Costas Fotakis, Anthi Ranella, Antonios G Kanaras, Emmanuel Stratakis
In this work, we report on a novel approach to develop hierarchically-structured cell culture platforms incorporating functionalized gold nanoparticles (AuNPs). In particular, the hierarchical substrates comprise primary pseudo-periodic arrays of silicon microcones combined with a secondary nanoscale pattern of homogeneously deposited AuNPs terminated with bio-functional moieties. AuNPs with various functionalities (i.e. oligopeptides, small molecules and oligomers) were successfully attached onto the microstructures...
April 6, 2018: Biomaterials Science
Maria Carles-Carner, Leila S Saleh, Stephanie Bryant
This study investigated the effects of introducing hydroxyapatite nanoparticles into a matrix metalloproteinase (MMP) sensitive poly(ethylene glycol) (PEG) hydrogel containing cell adhesion peptides of RGD for bone tissue engineering. MC3T3-E1 pre-osteoblasts were encapsulated in the biomimetic PEG hydrogel, which was formed from the photoclick thiol-norbornene reaction system, cultured for up to 28 days in growth medium or osteogenic differentiation medium, and evaluated by cellular morphology and differentiation by alkaline phosphatase activity and bone-like extracellular matrix (ECM) deposition for mineral and collagen...
April 3, 2018: Biomedical Materials
Xiao-Jing Shi, Yan-Yan Qiu, Hui Yu, Cheng Liu, Yu-Xia Yuan, Pei-Hao Yin, Tao Liu
A well-defined multifunctional brush-type polymeric prodrug covalently linked with an anticancer drug (bufalin, BUF), a tumor-targeting peptide (RGD), and an endosome-escaping polymer, poly(N,N-diethylaminoethyl methacrylate-co-butyl methacrylate (P(DEA-co-BMA)), was developed. Its anticancer performance against colon cancer was investigated in vitro and in vivo. Reversible addition-fragmentation transfer (RAFT) polymerization of oligo(ethylene glycol) monomethyl ether methacrylate (OEGMA), 2-((3-(tert-butoxy)-3-oxopropyl)thio)ethyl methacrylate (BSTMA), and 2-(2-bromoisobutyryloxy)ethylmethacrylate (BIEM) afforded the multifunctional random copolymer, P(OEGMA-co-BSTMA-co-BIEM), in which hydrophilic POEGMA can stabilize nanoparticles in water, PBSTMA can be converted into carboxyl groups, and PBIEM can be employed as a macromolecular atom radical transfer polymerization (ATRP) initiator...
March 19, 2018: Colloids and Surfaces. B, Biointerfaces
Dharmesh Patel, Sadhana Sharma, Hazel R C Screen, Stephanie J Bryant
We recently developed a fiber composite consisting of tenocytes seeded onto discontinuous fibers embedded within a hydrogel, designed to mimic physiological tendon micromechanics of tension and shear. This study examined if cell adhesion peptide (DGEA or YRGDS), fiber modulus (50 or 1300 kPa) and/or cyclic strain (5% strain, 1 Hz) influenced bovine tenocyte gene expression. Ten genes were analyzed and none were sensitive to peptide or fiber modulus in the absence of cyclic tensile strain. Genes associated with tendon (SCX and TNMD), collagens (COL1A1, COL3A1, COL11A1), and matrix remodelling (MMP1, MMP2, and TIMP3) were insensitive to cyclic strain...
March 27, 2018: Biochemical and Biophysical Research Communications
Hosni A M Hussein, Usama M Abdel-Raouf, Shaw M Akula
OBJECTIVES: Kaposi sarcoma-associated herpesvirus (KSHV) glycoprotein B (gB) is expressed on the viral envelope as well as on the cytoplasmic membrane of infected cells. In the current study, we aimed to decipher the impact of membrane-associated gB on adhesion and migration of cells via modulating the expression of cytokines. METHODS: A combination of polymerase chain reaction array, cell adhesion assay, and wound-healing migration assay was conducted to study the influence of the gB-induced cytokines on cell adhesion and migration...
March 29, 2018: Intervirology
Susan M Bello, Mary Shimoyama, Elvira Mitraka, Stanley J F Laulederkind, Cynthia L Smith, Janan T Eppig, Lynn M Schriml
Model organisms are vital to uncovering the mechanisms of human disease and developing new therapeutic tools. Researchers collecting and integrating relevant model organism and/or human data often apply disparate terminologies (vocabularies and ontologies), making comparisons and inferences difficult. A unified disease ontology is required that connects data annotated using diverse disease terminologies, and in which the terminology relationships are continuously maintained. The Mouse Genome Database (MGD, http://www...
March 12, 2018: Disease Models & Mechanisms
Hua Shi, Runqi Yan, Luyan Wu, Yidan Sun, Song Liu, Zhengyang Zhou, Jian He, Deju Ye
Precise diagnosis of lymph node metastasis to guide lymphadenectomy is highly important for gastric cancer therapy in clinics. Though surgical dissection of regional metastatic lymph nodes remains the only way for gastric cancer therapy, the extended dissection may cause unavoidable postoperative risk of complications. These is still lack of effective method enabling the accurate removal of metastatic gastric cancer cells in lymph nodes with minimum injuries to normal tissue. Herein, we report a new fluorescent copper sulfide (CuS) nanoparticle (RGD-CuS-Cy5...
March 24, 2018: Acta Biomaterialia
Ermelinda Lucente, Hongguang Liu, Yang Liu, Xiang Hu, Enza Lacivita, Marcello Leopoldo, Zhen Cheng
Bombesin receptor 2 (BB2 ) and integrin αv β3 receptor are privileged targets for molecular imaging of cancer because their overexpression in a number of tumor tissues. The most recent developments in heterodimer-based radiopharmaceuticals concern BB2 - and integrin αv β3 -targeting compounds, consisting of bombesin (BBN) and RGD peptides, connected through short length linkers. Molecular imaging probes based on RGD-BBN heterodimer design exhibit improved tumor targeting efficacy compared to the single-receptor targeting peptide monomers...
March 27, 2018: Bioconjugate Chemistry
Baochang Cheng, Yufei Yan, Jingjing Qi, Lianfu Deng, Zeng-Wu Shao, Ke-Qin Zhang, Bin Li, Ziling Sun, Xinming Li
Silk fibroin (SF) from Bombyx mori has received increasing interest in biomedical fields, because of its slow biodegradability, good biocompatibility, and low immunogenicity. Although SF based hydrogels have been studied intensively as potential matrix for tissue engineering, weak gelation performance and low mechanical strength are major limitations that hamper their widespread applicability. Therefore, searching for new strategies to improve SF gelation property is highly desirable in tissue engineering research...
March 27, 2018: ACS Applied Materials & Interfaces
Dongni Wu, Yongnu Zhang, Xiaoting Xu, Ting Guo, Deming Xie, Rong Zhu, Shengfeng Chen, Seeram Ramakrishna, Liumin He
In this study, we prepared a multifunctional gene delivery nanovector containing a chitosan (CS) backbone and polyethylenimine (PEI) arms with arginine-glycine-aspartate (RGD)/twin-arginine translocation (TAT) conjugated via polyethylene glycol (PEG). Branched PEI, with a molecular weight of 2000 Da, was used to achieve a balance between biocompatibility and transfection efficiency, whereas RGD/TAT peptides were conjugated for enhanced targeting ability and cellular uptake. Synthesis of the copolymers was confirmed by characterizing the chemical structure with1 H nuclear magnetic resonance and Fourier Transform Infrared Spectroscopy (FTIR)...
March 22, 2018: Acta Biomaterialia
Elizabeth A Aisenbrey, Stephanie J Bryant
Cartilage tissue engineering strategies that use in situ forming degradable hydrogels for mesenchymal stem cell (MSC) delivery are promising for treating chondral defects. Hydrogels that recapitulate aspects of the native tissue have the potential to encourage chondrogenesis, permit cellular mediated degradation, and facilitate tissue growth. This study investigated photoclickable poly(ethylene glycol) (PEG) hydrogels, which were tailored to mimic the cartilage microenvironment by incorporating extracellular matrix analogs, chondroitin sulfate and RGD, and crosslinks sensitive to matrix metalloproteinase 7 (MMP7)...
March 25, 2018: Journal of Biomedical Materials Research. Part A
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