keyword
MENU ▼
Read by QxMD icon Read
search

RGD

keyword
https://www.readbyqxmd.com/read/27922023/systematic-site-directed-mutagenesis-of-the-helicobacter-pylori-cagl-protein-of-the-cag-type-iv-secretion-system-identifies-novel-functional-domains
#1
Tobias Bönig, Patrick Olbermann, Simon H Bats, Wolfgang Fischer, Christine Josenhans
The Cag Type IV secretion system, which contributes to inflammation and cancerogenesis during chronic infection, is one of the major virulence factors of the bacterial gastric pathogen Helicobacter pylori. We have generated and characterized a series of non-marked site-directed chromosomal mutants in H. pylori to define domains of unknown function of the essential tip protein CagL of the Cag secretion system. Characterizing the CagL mutants, we determined that their function to activate cells and transport the effector CagA was reduced to different extents...
December 6, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27921110/multifunctional-pei-entrapped-gold-nanoparticles-enable-efficient-delivery-of-therapeutic-sirna-into-glioblastoma-cells
#2
Lingdan Kong, Jieru Qiu, Wenjie Sun, Jia Yang, Mingwu Shen, Lu Wang, Xiangyang Shi
RNA interference (RNAi) has been considered as a promising strategy for effective treatment of cancer. However, the easy degradation of small interfering RNA (siRNA) limits its extensive applications in gene therapy. For safe and effective delivery of siRNA, a novel vector system possessing excellent biocompatibility, highly efficient transfection efficiency and specific targeting properties has to be considered. In this study, we report the use of polyethyleneimine (PEI)-entrapped gold nanoparticles (Au PENPs) modified with an arginine-glycine-aspartic (Arg-Gly-Asp, RGD) peptide via a poly(ethylene glycol) (PEG) spacer as a vector for Bcl-2 (B-cell lymphoma-2) siRNA delivery to glioblastoma cells...
December 6, 2016: Biomaterials Science
https://www.readbyqxmd.com/read/27918306/targeting-integrin-%C3%AE-5%C3%AE-1-ameliorates-severe-airway-hyperresponsiveness-in-experimental-asthma
#3
Aparna Sundaram, Chun Chen, Amin Khalifeh-Soltani, Amha Atakilit, Xin Ren, Wenli Qiu, Hyunil Jo, William DeGrado, Xiaozhu Huang, Dean Sheppard
Treatment options are limited for severe asthma, and the need for additional therapies remains great. Previously, we demonstrated that integrin αvβ6-deficient mice are protected from airway hyperresponsiveness, due in part to increased expression of the murine ortholog of human chymase. Here, we determined that chymase protects against cytokine-enhanced bronchoconstriction by cleaving fibronectin to impair tension transmission in airway smooth muscle (ASM). Additionally, we identified a pathway that can be therapeutically targeted to mitigate the effects of airway hyperresponsiveness...
December 5, 2016: Journal of Clinical Investigation
https://www.readbyqxmd.com/read/27907905/association-of-high-microvessel-%C3%AE-v%C3%AE-3-and-low-pten-with-poor-outcome-in-stage-3-neuroblastoma-rationale-for-using-first-in-class-dual-pi3k-brd4-inhibitor-sf1126
#4
Anat Erdreich-Epstein, Alok R Singh, Shweta Joshi, Francisco M Vega, Pinzheng Guo, Jingying Xu, Susan Groshen, Wei Ye, Melissa Millard, Mihaela Campan, Guillermo Morales, Joseph R Garlich, Peter W Laird, Robert C Seeger, Hiroyuki Shimada, Donald L Durden
Neuroblastoma (NB) is the most common extracranial solid tumor in children. Our previous studies showed that the angiogenic integrin αvβ3 was increased in high-risk metastatic (stage 4) NB compared with localized neuroblastomas. Herein, we show that integrin αvβ3 was expressed on 68% of microvessels in MYCN-amplified stage 3 neuroblastomas, but only on 34% (means) in MYCN-non-amplified tumors (p < 0.001; n = 54). PTEN, a tumor suppressor involved in αvβ3 signaling, was expressed in neuroblastomas either diffusely, focally or not at all (immunohistochemistry)...
November 16, 2016: Oncotarget
https://www.readbyqxmd.com/read/27904082/design-and-synthesis-of-non-peptide-rgd-mimics-for-evaluation-of-their-utility-as-anti-platelet-agents
#5
Kazuhiro Higuchi, Hideki Hikita, Asumi Murayama, Daichi Yuri, Natsu Kobayakawa, Takashi Takahashi, Shigeru Kojima, Hiroko Ueno, Tomomi Hatakeyama, Airi Kato, Masanori Tayu, Etsuko Oyama, Shigeo Sugiyama, Kazuyuki Ishii, Hidenobu Takahashi, Tomomi Kawasaki
Arg-Gly-Asp (RGD) mimics were synthesized and their anti-platelet activity was evaluated. A concise method was developed for the synthesis of the target compounds from dehydroepiandrosterone and Wieland-Miescher and Hajos-Parrish ketones, which are suitable for readily available platform. Among the synthesized compounds, the perhydronaphthalene framework with a 3-(4-piperidinyl)propoxyl structure 3e possessed the highest anti-aggregative activity. The IC50 values of 3e were 0.91 mM (ADP initiation) and 0...
2016: Chemical & Pharmaceutical Bulletin
https://www.readbyqxmd.com/read/27901317/dendrimer-stabilized-gold-nanostars-as-a-multifunctional-theranostic-nanoplatform-for-ct-imaging-photothermal-therapy-and-gene-silencing-of-tumors
#6
Ping Wei, Jingwen Chen, Yong Hu, Xin Li, Han Wang, Mingwu Shen, Xiangyang Shi
Development of versatile nanomaterials combining diagnostic and therapeutic functionalities within one single nanoplatform is extremely important for tumor theranostics. In this work, the authors report the synthesis of a gold nanostar (Au NS)-based theranostic platform stabilized with cyclic arginine-glycine-aspartic (Arg-Gly-Asp, RGD) peptide-modified amine-terminated generation 3 poly(amidoamine) dendrimers. The formed RGD-modified dendrimer-stabilized Au NSs (RGD-Au DSNSs) are used as a gene delivery vector to complex small interfering RNA (siRNA) for computed tomography (CT) imaging, thermal imaging, photothermal therapy (PTT), and gene therapy of tumors...
November 30, 2016: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/27899989/tumor-penetration-and-antitumor-efficacy-of-cetuximab-are-enhanced-by-co-administered-irgd-in-a-murine-model-of-human-nsclc
#7
Yang Zhang, Jie Yang, Manhua Ding, Liantao Li, Zheng Lu, Qing Zhang, Junnian Zheng
Lung cancer is the leading cause of cancer-associated mortality, worldwide. For this reason, novel therapies are required for the treatment of this devastating disease. Cetuximab is a monoclonal antibody against epidermal growth factor receptor (EGFR), which is overexpressed in a variety of solid tumors, including non-small cell lung cancer (NSCLC). The therapeutic efficacy of cetuximab for NSCLC is limited to use as a monotherapy or in combination with chemotherapy. The objective of the present study was to develop a novel strategy to enhance the therapeutic efficacy of cetuximab for NSCLC by a co-administration with the tumor-penetrating internalizing RGD peptide (iRGD)...
November 2016: Oncology Letters
https://www.readbyqxmd.com/read/27895477/noninvasive-monitoring-of-early-antiangiogenic-therapy-response-in-human-nasopharyngeal-carcinoma-xenograft-model-using-mri-with-rgd-conjugated-ultrasmall-superparamagnetic-iron-oxide-nanoparticles
#8
Yanfen Cui, Caiyuan Zhang, Ran Luo, Huanhuan Liu, Zhongyang Zhang, Tianyong Xu, Yong Zhang, Dengbin Wang
PURPOSE: Arginine-glycine-aspartic acid (RGD)-based nanoprobes allow specific imaging of integrin αvβ3, a protein overexpressed during angiogenesis. Therefore, this study applied a novel RGD-coupled, polyacrylic acid (PAA)-coated ultrasmall superparamagnetic iron oxide (USPIO) (referred to as RGD-PAA-USPIO) in order to detect tumor angiogenesis and assess the early response to antiangiogenic treatment in human nasopharyngeal carcinoma (NPC) xenograft model by magnetic resonance imaging (MRI)...
2016: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/27894082/the-disintegrin-echistatin-in-combination-with-doxorubicin-targets-high-metastatic-human-osteosarcoma-overexpressing-%C3%AE-%C3%AE-%C3%AE-3-integrin-in-chick-embryo-and-nude-mouse-models
#9
Yasunori Tome, Hiroaki Kimura, Naotoshi Sugimoto, Hiroyuki Tsuchiya, Fuminori Kanaya, Michael Bouvet, Robert M Hoffman
Echistatin, a cyclic RGD peptide, which is an antagonist of αvβ3 integrin (disintegrin), inhibited human osteosarcoma in the chick chorioallontoic membrane (CAM) model and tumor growth and pulmonary metastases in a nude mouse orthotopic model. A high-metastatic variant of human osteosarcoma, 143B-LM4, overexpressing αvβ3 integrin was used. Tumor angiogenesis by high-metastatic variant 143B-LM4 cells in the CAM was significantly inhibited by echistatin (P<0.05) as was overall growth. A doxorubicin (DOX)-echistatin combination inhibited orthotopic tumor growth compared to untreated control (P<0...
November 22, 2016: Oncotarget
https://www.readbyqxmd.com/read/27893196/guiding-hmsc-adhesion-and-differentiation-on-supported-lipid-bilayers
#10
Gülistan Koçer, Pascal Jonkheijm
Mesenchymal stem cells (MSCs) are intensively investigated for regenerative medicine applications due to their ease of isolation and multilineage differentiation capacity. Hence, designing instructive microenvironments to guide MSC behavior is important for the generation of smart interfaces to enhance biomaterial performance in guiding desired tissue formation. Supported lipid bilayers (SLBs) as cell membrane mimetics can be employed as biological interfaces with easily tunable characteristics such as biospecificity, mobility, and density of predesigned ligand molecules...
November 28, 2016: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/27889664/macrophage-integrins-modulate-response-to-ultra-high-molecular-weight-polyethylene-particles-and-direct-particle-induced-osteolysis
#11
Toral D Zaveri, Natalia V Dolgova, Jamal S Lewis, Kiri Hamaker, Michael J Clare-Salzler, Benjamin G Keselowsky
Aseptic loosening due to peri-prosthetic osteolysis is one of the primary causes for failure of artificial joint replacements. Implant-derived wear particles, often ultra-high molecular weight polyethylene (UHMWPE) microparticles, initiate an inflammatory cascade upon phagocytosis by macrophages, which leads to osteoclast recruitment and activation, ultimately resulting in osteolysis. Investigation into integrin receptors, involved in cellular interactions with biomaterial-adsorbed adhesive proteins, is of interest to understand and modulate inflammatory processes...
October 27, 2016: Biomaterials
https://www.readbyqxmd.com/read/27881070/pregnancy-specific-%C3%AE-1-glycoproteins-combined-biomarker-roles-structure-function-relationships-and-implications-for-drug-design
#12
Nurbubu T Moldogazieva, Innokenty M Mokhosoev, Alexander A Terentiev
BACKGROUND: Pregnancy specific β1-glycoproteins (PSGs) have long been recognized as trophoblast quality and embryo viability markers. However, biological roles of PSGs remain obscure, and structure/function relationships to other feto-placental proteins as well as implications for drug design have not been reviewed. This review summarizes and discusses advances in 45-year studies of PSGs with focus on the latest achievements and the challenges for future investigations. METHODS: Literature search was performed to review the majority of recent PSG studies with emphasis on usage of high-throughput integrated proteomic profiling technologies, systems biology and bioinformatics approaches that enhance novel biomarker and drug target discovery as well as protein structure/activity analysis...
November 22, 2016: Current Medicinal Chemistry
https://www.readbyqxmd.com/read/27879373/novel-molecular-add-on-based-on-evans-blue-confers-superior-pharmacokinetics-and-transforms-drugs-to-theranostic-agents
#13
Haojun Chen, Orit Jacobson, Gang Niu, Ido Weiss, Dale O Kiesewetter, Yi Liu, Ying Ma, Hua Wu, Xiaoyuan Chen
: One of the major design considerations for a drug is its pharmacokinetics in the blood. A drug with short half-life in the blood is less available at a target organ. Such a limitation dictates treatment with either high doses or more frequent doses, both of which may increase the likelihood of undesirable side effects. To address the need for additional methods to improve the blood half-life of drugs and molecular imaging agents, we developed an "add-on" molecule that contains three groups: (i) truncated Evans Blue (EB) dye molecule that binds to albumin with low micromolar affinity and provides prolonged half-life in the blood; (ii) a metal chelate that allows radiolabeling for imaging and radiotherapy; and (iii) a maleimide for easy conjugation to drug molecules...
November 22, 2016: Journal of Nuclear Medicine: Official Publication, Society of Nuclear Medicine
https://www.readbyqxmd.com/read/27878899/conformational-analysis-of-a-modified-rgd-adhesive-sequence
#14
Jordi Triguero, David Zanuy, Carlos Alemán
The conformational preferences of the Arg-GlE-Asp sequence, where GlE is an engineered amino acid bearing a 3,4-ethylenedioxythiophene (EDOT) ring as side group, have been determined combining density functional theory calculations with a well-established conformational search strategy. Although the Arg-GlE-Asp sequence was designed to prepare a conducting polymer-peptide conjugate with excellent electrochemical and bioadhesive properties, the behavior of such hybrid material as adhesive biointerface is improvable...
November 23, 2016: Journal of Peptide Science: An Official Publication of the European Peptide Society
https://www.readbyqxmd.com/read/27877040/intestinal-anti-inflammatory-effects-of-rgd-functionalized-silk-fibroin-nanoparticles-in-trinitrobenzenesulfonic-acid-induced-experimental-colitis-in-rats
#15
Alba Rodriguez-Nogales, Francesca Algieri, Laura De Matteis, A Abel Lozano-Perez, Jose Garrido-Mesa, Teresa Vezza, J M de la Fuente, Jose Luis Cenis, Julio Gálvez, Maria Elena Rodriguez-Cabezas
BACKGROUND: Current treatment of inflammatory bowel disease is based on the use of immunosuppressants or anti-inflammatory drugs, which are characterized by important side effects that can limit their use. Previous research has been performed by administering these drugs as nanoparticles that target the ulcerated intestinal regions and increase their bioavailability. It has been reported that silk fibroin can act as a drug carrier and shows anti-inflammatory properties. PURPOSE: This study was designed to enhance the interaction of the silk fibroin nanoparticles (SFNs) with the injured intestinal tissue by functionalizing them with the peptide motif RGD (arginine-glycine-aspartic acid) and to evaluate the intestinal anti-inflammatory properties of these RGD-functionalized silk fibroin nanoparticles (RGD-SFNs) in the trinitrobenzenesulfonic acid (TNBS) model of rat colitis...
2016: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/27867754/a-small-chimeric-fibronectin-fragment-accelerates-dermal-wound-repair-in-diabetic-mice
#16
Denise C Hocking, James R Brennan, Carol H Raeman
Objective: During wound repair, soluble fibronectin is converted into biologically active, insoluble fibrils via a cell-mediated process. This fibrillar, extracellular matrix (ECM) form of fibronectin stimulates cell processes critical to tissue repair. Nonhealing wounds show reduced levels of ECM fibronectin fibrils. The objective of this study was to produce a small, recombinant wound supplement with the biological activity of insoluble fibronectin fibrils. Approach: A chimeric fibronectin fragment was produced by inserting the integrin-binding Arg-Gly-Asp (RGD) loop from the tenth type III repeat of fibronectin (FNIII10) into the analogous site within the heparin-binding, bioactive fragment of the first type III repeat (FNIII1H)...
November 1, 2016: Advances in Wound Care
https://www.readbyqxmd.com/read/27865044/cyclo-rgd-decorated-reduction-responsive-nanogels-mediate-targeted-chemotherapy-of-integrin-overexpressing-human-glioblastoma-in-vivo
#17
Wei Chen, Yan Zou, Zhiyuan Zhong, Rainer Haag
Cyclo(Arg-Gly-Asp) peptide (cRGD) decorated disulfide (SS) containing poly(vinyl alcohol) nanogels (cRGD-SS-NGs) with an average diameter of 142 nm prepared by inverse nanoprecipitation, "click" reaction, and cRGD conjugation are developed for targeted treatment of integrin overexpressing human glioblastoma in vivo. Doxorubicin (DOX) release from cRGD-SS-NGs is highly inhibited under physiological conditions, while accelerated at endosomal pH and in response to cytoplasmic concentration of glutathione. Confocal microscopy shows that cRGD-SS-NGs facilitate the cellular uptake and intracellular DOX release in αv β3 integrin overexpressing human glioblastoma U87-MG cells...
November 16, 2016: Small
https://www.readbyqxmd.com/read/27864999/a-synthetic-toolbox-for-the-in-situ-formation-of-functionalized-homo-and-heteropolysaccharide-based-hydrogel-libraries
#18
Nick Dibbert, Andreas Krause, Julio-Cesar Rios-Camacho, Ina Gruh, Andreas Kirschning, Gerald Dräger
A synthetic toolbox for the introduction of aldehydo and hydrazido groups into the polysaccharides hyaluronic acid, alginate, dextran, pullulan, glycogen, and carboxymethyl cellulose and their use for hydrogel formation is reported. Upon mixing differently functionalized polysaccharides derived from the same natural precursor, hydrazone cross-linking takes place, which results in formation of a hydrogel composed of one type of polysaccharide backbone. Likewise, hydrogels based on two different polysaccharide strands can be formed after mixing the corresponding aldehydo- and hydrazido-modified polysaccharides...
November 16, 2016: Chemistry: a European Journal
https://www.readbyqxmd.com/read/27864802/secreted-phospholipase-a2-type-iia-spla2-iia-activates-integrins-in-an-allosteric-manner
#19
Yoshikazu Takada, Masaaki Fujita
Secreted phospholipase A2 type IIA (sPLA2-IIA) is a well-established pro-inflammatory protein and has been a major target for drug discovery. However, the mechanism of its signaling action has not been fully understood. We previously found that sPLA2-IIA binds to integrins αvβ3 and α4β1 in human and that this interaction plays a role in sPLA2-IIA's signaling action. Our recent studies found that sPLA2-IIA activates integrins in an allosteric manner through direct binding to a newly identified binding site of integrins (site 2), which is distinct from the classical RGD-binding site (site 1)...
November 19, 2016: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/27864602/beyond-bread-and-beer-whole-cell-protein-extracts-from-baker-s-yeast-as-a-bulk-source-for-3d-cell-culture-matrices
#20
Nicholas Bodenberger, Dennis Kubiczek, Patrick Paul, Nico Preising, Lukas Weber, Ramona Bosch, Rudolf Hausmann, Kay-Eberhard Gottschalk, Frank Rosenau
Here, we present a novel approach to form hydrogels from yeast whole cell protein. Countless hydrogels are available for sophisticated research, but their fabrication is often difficult to reproduce, with the gels being complicated to handle or simply too expensive. The yeast hydrogels presented here are polymerized using a four-armed, amine reactive crosslinker and show a high chemical and thermal resistance. The free water content was determined by measuring swelling ratios for different protein concentrations, and in a freeze-drying approach, pore sizes of up to 100 μm in the gel could be created without destabilizing the 3D network...
November 18, 2016: Applied Microbiology and Biotechnology
keyword
keyword
27894
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"