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Caifeng Ding, Yujuan Xu, Yanan Zhao, Hua Zhong, Xiliang Luo
Drug delivery which can offer efficient and localized drug transportation together with imaging capabilities is highly demanded in the development of cancer theranostic approaches. Herein, we report the construction of bovine serum albumin (BSA) gold nanoclusters (BSA@AuNCs) for cell fluoresce imaging and target drug delivery. BSA@AuNCs were modified with cyclic arginine-glycine-aspartate (cRGD) with the product RGD-BSA@AuNCs to enhance the cell internalization of the nanoclusters. Furthermore, doxorubicin (DOX), a widely used a chemotherapy drug, was also used to modify RGD-BSA@AuNCs...
February 19, 2018: ACS Applied Materials & Interfaces
Fu-Chen Kung
Imbalance crosslink density and polymer concentration gradient is formed within the traditional alginate hydrogel using calcium chloride as a crosslinking agent in external gelation for instantaneously process. In this studying, type I collagen (Col I) blended calcium salt form of poly(γ-glutamic acid) (γCaPGA) was mixing with RGD-modified alginate with convenient gelation process and suitable for practical use. The hydrophilicity of the resulting hydrogels was evaluated through swelling tests, water retention capacity tests, and water vapor permeation tests...
2018: Bio-medical Materials and Engineering
Dongdong Bi, Lei Zhao, Runqi Yu, Haowen Li, Yifei Guo, Xiangtao Wang, Meihua Han
One major challenge of current surface modification of nanoparticles is the demand for chemical reactive polymeric layers, such modification is always complicated, inefficient, and may lead the polymer lose the ability to encapsulate drug. To overcome this limitation, we adopted a pH-sensitive platform using polydopamine (PDA) as a way of functionalizing nanoparticles (NPs) surfaces. All this method needed to be just a brief incubation in weak alkaline solution of dopamine, which was simple and applicable to a variety of polymer carriers regardless of their chemical reactivity...
November 2018: Drug Delivery
Rhiannon R Batchelor, Eva Blasco, Kilian N R Wuest, Hongxu Lu, Martin Wegener, Christopher Barner-Kowollik, Martina H Stenzel
A λ-orthogonal reaction system is introduced, where visible light induced radical thiol-ene and UV light induced NITEC (Nitrile-Imine mediated Tetrazole-Ene Conjugation) ligations are consecutively employed to fabricate and functionalize PEG-based hydrogels. The fluorescent pyrazoline cycloadducts from the NITEC reaction are exploited to visualize the written structures within the hydrogels as well as to attach RGD containing functional groups to promote spatially resolved cell attachment on the hydrogel surface...
February 19, 2018: Chemical Communications: Chem Comm
Zuyuan Luo, Siqi Zhang, Jijia Pan, Rui Shi, Hao Liu, Yalin Lyu, Xiao Han, Yan Li, Yue Yang, Zhixiu Xu, Yi Sui, En Luo, Yuanyuan Zhang, Shicheng Wei
The efficacy of stem cell-based bone tissue engineering has been hampered by cell death and limited fate control. A smart cell culture system with the capability of sequentially delivering multiple factors in specific growth stages, like the mechanism of the natural extracellular matrix modulating tissue formation, is attractive for enhancing cell activity and controlling cell fate. Here, a bone forming peptide-1 (BFP-1)-laden mesoporous silica nanoparticles (pep@MSNs) incorporated adhesion peptide, containing the arginine-glycine-aspartic acid (RGD) domain, modified alginate hydrogel (RA) system (pep@MSNs-RA) was developed to promote the activity and stimulate osteo-differentiation of human mesenchymal stem cells (hMSCs) in sequence...
February 10, 2018: Biomaterials
Tessa Buckle, Danny M van Willigen, Silvia J Spa, Albertus W Hensbergen, Steffen van der Wal, Clarize M de Korne, Mick M Welling, Henk G van der Poel, James C H Hardwick, Fijs W B van Leeuwen
Objectives: The potential of (receptor-mediated) fluorescence-based image-guided surgery tracers is generally linked to the near-infrared emission profile and good manufacturing production (GMP) availability of fluorescent dyes. Surprisingly, little is known about the critical interaction between the structural composition of the dye and the pharmacokinetics of the tracers. In this study, a bimodal/hybrid tracer design was used to systematically and quantitatively evaluate the influence of elongation of the polymethine chain in a fluorescent cyanine (Cy) dye on the imaging potential of a targeted (RGD-based) tracer...
February 15, 2018: Journal of Nuclear Medicine: Official Publication, Society of Nuclear Medicine
Zhi-Jian Sun, Yi-Zhe Zhang, Fan Liu, Juan-Juan Chen, Dong-Xue Chen, Hong-Bao Liu, Liang Liang, Hua Han
Intestine is vulnerable to irradiation injury, which induces cell death and compromises regeneration of intestinal crypts. It is well accepted that cryptic stem cells, which are responsible for cryptic regeneration under physiological and pathological conditions, are controlled by multiple cell-intrinsic and environmental signals such as Notch signaling. Therefore in this study, we tested whether a soluble Notch ligand tethered to endothelial cells -- mD1R -- ¬¬¬the DSL domain of mouse Notch ligand Delta-like1 fused with a RGD motif, could protect cryptic cells from irradiation-induced intestinal injury...
February 14, 2018: Bioscience Reports
Ignasi Casanellas, Anna Lagunas, Iro Tsintzou, Yolanda Vida, Daniel Collado, Ezequiel Pérez-Inestrosa, Cristina Rodríguez-Pereira, Joana Magalhaes, Pau Gorostiza, José A Andrades, José Becerra, Josep Samitier
Cellular adhesion and differentiation is conditioned by the nanoscale disposition of the extracellular matrix (ECM) components, with local concentrations having a major effect. Here we present a method to obtain large-scale uneven nanopatterns of arginine-glycine-aspartic acid (RGD)-functionalized dendrimers that permit the nanoscale control of local RGD surface density. Nanopatterns are formed by surface adsorption of dendrimers from solutions at different initial concentrations and are characterized by water contact angle (CA), X-ray photoelectron spectroscopy (XPS), and scanning probe microscopy techniques such as scanning tunneling microscopy (STM) and atomic force microscopy (AFM)...
January 20, 2018: Journal of Visualized Experiments: JoVE
Frederick F Lang, Charles Conrad, Candelaria Gomez-Manzano, W K Alfred Yung, Raymond Sawaya, Jeffrey S Weinberg, Sujit S Prabhu, Ganesh Rao, Gregory N Fuller, Kenneth D Aldape, Joy Gumin, Luis M Vence, Ignacio Wistuba, Jaime Rodriguez-Canales, Pamela A Villalobos, Clemens M F Dirven, Sonia Tejada, Ricardo D Valle, Marta M Alonso, Brett Ewald, Joanna J Peterkin, Frank Tufaro, Juan Fueyo
Purpose DNX-2401 (Delta-24-RGD; tasadenoturev) is a tumor-selective, replication-competent oncolytic adenovirus. Preclinical studies demonstrated antiglioma efficacy, but the effects and mechanisms of action have not been evaluated in patients. Methods A phase I, dose-escalation, biologic-end-point clinical trial of DNX-2401 was conducted in 37 patients with recurrent malignant glioma. Patients received a single intratumoral injection of DNX-2401 into biopsy-confirmed recurrent tumor to evaluate safety and response across eight dose levels (group A)...
February 12, 2018: Journal of Clinical Oncology: Official Journal of the American Society of Clinical Oncology
Yan Zhu, Dongqing Wang, Zhe Liu, Yuefeng Li
BACKGROUND: Neuroimaging studies have shown that major depressive disorder is associated with altered activity patterns of the default-mode network (DMN). In this study, we sought to investigate the topological organization of the DMN in patients with remitted geriatric depression (RGD) and whether RGD patients would be more likely to show disrupted topological configuration of the DMN during the resting-state. METHODS: Thirty-three RGD patients and thirty-one healthy control participants underwent clinical and cognitive evaluations as well as resting-state functional magnetic resonance imaging scans...
February 12, 2018: International Psychogeriatrics
Guangjian Zhang, Rui Gao, Yuanbo Wang, Yan Liu, Juan Li, Xi Jia, Yiqian Liang, Aimin Yang
Aims: To investigate the association between angiogenetic activity of hyperplastic thymus and serum thyroglobulin (Tg) level in differentiated thyroid carcinoma patients with thyroglobulin (Tg)-elevated Negative Iodine Scintigraphy (TENIS) Syndrome. Methods: A cohort of 30 consecutive patients who underwent total thyroidectomy followed by radioiodine ablation and had TENIS syndrome received integrin αvβ3 targeted imaging with 99mTc-HYNIC-PEG4-E[PEG4-c(RGDfk)]2 (99mTc-3PRGD2)...
January 9, 2018: Oncotarget
Nick Huettner, Tim R Dargaville, Aurelien Forget
As an alternative to natural extracellular matrix (ECM) macromolecules, cell-adhesion peptides (CAPs) have had tremendous impact on the design of cell culture platforms, implants, and wound dressings. However, only a handful of CAPs have been utilized. The discrepancy in ECM composition strongly affects cell behavior, so it is paramount to reproduce such differences in synthetic systems. This Opinion article presents strategies inspired from high-throughput screening techniques implemented in drug discovery to exploit the potential of a growing CAP library...
February 5, 2018: Trends in Biotechnology
Nengpan Zhang, Chunlin Li, Dayong Zhou, Chen Ding, Yaqing Jin, Qingmei Tian, Xiangzhou Meng, Kefeng Pu, Yimin Zhu
Thrombosis, a critical event in blood vessels, not only is associated with myocardial infarction and stroke, but also accounts for considerable morbidity and mortality. Thrombolytic drugs are usually applied to the treatment of acute myocardial infarction, acute cerebral infarction and pulmonary embolism. However, thrombolytic drugs show limited efficacy in clinical practice because of the short half-life in plasma and systemic side effects. In this study, the cyclic RGD (cRGD) functionalized liposomes were prepared to encapsulate urokinase, a cheap and widely used thrombolytic drug in clinic and better thrombolysis efficacy was achieved...
February 2, 2018: Acta Biomaterialia
Nathaniel J Hogrebe, James W Reinhardt, Nguyen K Tram, Anna C Debski, Gunjan Agarwal, Matthew A Reilly, Keith J Gooch
A cell's insoluble microenvironment has increasingly been shown to exert influence on its function. In particular, matrix stiffness and adhesiveness strongly impact behaviors such as cell spreading and differentiation, but materials that allow for independent control of these parameters within a fibrous, stromal-like microenvironment are very limited. In the current work, we devise a self-assembling peptide (SAP) system that facilitates user-friendly control of matrix stiffness and RGD (Arg-Gly-Asp) concentration within a hydrogel possessing a microarchitecture similar to stromal extracellular matrix...
February 1, 2018: Acta Biomaterialia
Shengnan Wang, Junshu Jiang, Yue Wang, Qilan Jia, Shufang Dai, Yuping Wang, Li Lv, Jihong Wang
Recombinant Lampetra japonica RGD-peptide (rLj-RGD3), a soluble protein containing three RGD sequences, was acquired from the oral salivary glands of Lampetra japonica using recombinant DNA technology. The aim of this study was to investigate the protective effects of rLj-RGD3 against acute myocardial infarction (AMI) induced by coronary artery thrombosis, as well as the underlying mechanisms. A rat model of AMI caused by ferric chloride-induced thrombosis on the surface of the left anterior descending (LAD) coronary artery was successfully established...
February 3, 2018: Biochemical and Biophysical Research Communications
Simona Morochnik, Yunxiao Zhu, Chongwen Duan, Michelle Cai, Russell R Reid, Tong-Chuan He, Jason Koh, Igal Szleifer, Guillermo A Ameer
There is a need in orthopaedic and craniomaxillofacial surgeries for materials that are easy to handle and apply to a surgical site, can fill and fully conform to the bone defect, and can promote the formation of new bone tissue. Thermoresponsive polymers that undergo liquid to gel transition at physiological temperature can potentially be used to meet these handling and shape-conforming requirements. However, there are no reports on their capacity to induce in vivo bone formation. The objective of this research was to investigate whether the functionalization of the thermoresponsive, antioxidant macromolecule poly(poly-ethyleneglycol citrate-co-N-isopropylacrylamide) (PPCN), with strontium, phosphate, and/or the cyclic RGD peptide would render it a hydrogel with osteoinductive properties...
February 2, 2018: Journal of Biomedical Materials Research. Part A
Xianchun Wen, Jiping Li, Defu Cai, Liling Yue, Qi Wang, Li Zhou, Li Fan, Jianwen Sun, Yonghui Wu
Shikonin (SHK) has been proven to have a good anti-tumor effect. However, poor water solubility and low bioavailability limit its wide application in clinical practice. In this study, to overcome these drawbacks, RGD-modified shikonin-loaded liposomes (RGD-SSLs-SHK) were successfully prepared. It exhibited excellent physicochemical characteristics including particle size, zeta potential, encapsulation efficiency, and delayed release time. Meanwhile, the targeting activity of the RGD-modified liposomes was demonstrated by flow cytometry and confocal microscopy in the αvβ₃-positive MDA-MB-231 cells...
January 29, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Elliott E Hill, Jin Koo Kim, Younghun Jung, Chris K Neeley, Kenneth J Pienta, Russell S Taichman, Jacques E Nor, James R Baker, Paul H Krebsbach
Therapeutic strategies targeting both cancer cells and associated cells in the tumor microenvironment offer significant promise in cancer therapy. We previously reported that generation 5 (G5) dendrimers conjugated with cyclic-RGD peptides target cells expressing integrin alpha V beta 3. In this study, we report a novel dendrimer conjugate modified to deliver the mammalian target of rapamycin (mTOR) inhibitor, rapamycin. In vitro analyses demonstrated that this drug conjugate, G5-FI-RGD-rapamycin, binds to prostate cancer (PCa) cells and fibroblasts to inhibit mTOR signaling and VEGF expression...
January 30, 2018: Journal of Cellular Biochemistry
Benqing Zhou, Ruizhi Wang, Feng Chen, Lingzhou Zhao, Peng Wang, Xin Li, István Bányai, Qiang Ouyang, Xiangyang Shi, Mingwu Shen
We report the construction and characterization of99m Tc-labeled arginine-glycine-aspartic acid (RGD)-polyethylenimine (PEI) conjugates with entrapped gold nanoparticles in the cavities (RGD-99m Tc-Au PENPs) for dual-mode single-photon emission computed tomography (SPECT)/computed tomography (CT) imaging of an orthotopic hepatic carcinoma model. In this study, PEI was successively decorated with diethylenetriaminepentaacetic acid, poly(ethylene glycol) (PEG), and PEGylated RGD segments, and was utilized as an effective nanoplatform to entrap Au NPs and to be labeled with99m Tc...
February 9, 2018: ACS Applied Materials & Interfaces
Adam Richardson, Lewis Muir, Sasha Mousdell, Darren Sexton, Sarah Jones, John Howl, Kehinde Ross
OBJECTIVE: Biologically active cell penetrating peptides (CPPs) are an emerging class of therapeutic agent. The wasp venom peptide mastoparan is an established CPP that modulates mitochondrial activity and triggers caspase-dependent apoptosis in cancer cells, as does the mastoparan analogue mitoparan (mitP). Mitochondrial depolarisation and activation of the caspase cascade also underpins the action of dithranol, a topical agent for treatment of psoriasis. The effects of a potent mitP analogue on mitochondrial activity were therefore examined to assess its potential as a novel approach for targeting mitochondria for the treatment of psoriasis...
January 30, 2018: BMC Research Notes
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