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https://www.readbyqxmd.com/read/29913479/prioritizing-and-modelling-of-putative-drug-target-proteins-of-candida-albicans-by-systems-biology-approach
#1
Tariq Ismail, Nighat Fatima, Syed Aun Muhammad, Syed Saoud Zaidi, Nisar Rehman, Izhar Hussain, Najam Us Sahr Tariq, Imran Amirzada, Abdul Mannan
Candida albicans (Candida albicans) is one of the major sources of nosocomial infections in humans which may prove fatal in 30% of cases. The hospital acquired infection is very difficult to treat affectively due to the presence of drug resistant pathogenic strains, therefore there is a need to find alternative drug targets to cure this infection. In silico and computational level frame work was used to prioritize and establish antifungal drug targets of Candida albicans. The identification of putative drug targets was based on acquiring 5090 completely annotated genes of Candida albicans from available databases which were categorized into essential and non-essential genes...
2018: Acta Biochimica Polonica
https://www.readbyqxmd.com/read/29913331/unraveling-the-binding-interaction-of-a-bioactive-pyrazole-based-probe-with-serum-proteins-relative-concentration-dependent-1-1-and-2-1-probe-protein-stoichiometries
#2
Pronab Kundu, Nitin Chattopadhyay
Molecular interactions and binding of probes/drugs with biomacromolecular systems are of fundamental importance in understanding the mechanism of action and hence designing of proactive drugs. In the present study, binding interactions of a biologically potent fluorophore, (E)-1,5-diphenyl-3-styryl-4,5-dihydro-1H-pyrazole (DSDP) with two serum transport proteins, human serum albumin and bovine serum albumin, have been investigated exploiting multi-spectroscopic techniques. The spectrophotometric and fluorometric studies together with fluorescence quenching, fluorescence anisotropy, urea induced denaturation studies and fluorescence lifetime measurements reveal strong binding of DSDP with both the plasma proteins...
June 15, 2018: Biophysical Chemistry
https://www.readbyqxmd.com/read/29912261/-mexican-case-of-tinea-incognito-and-granuloma-de-majocchi-acquired-from-a-hedgehog
#3
Lorena Lammoglia-Ordiales, Erick Martínez-Herrera, Sonia Toussaint-Caire, Roberto Arenas, Gabriela Moreno-Coutiño
Exotic pets, such as the ground hedgehog, are capable of transmitting to the human being different zoonoses, such as salmonellosis, mycobacteria, protozoa such as Cryptosporidium parvum, and dermatophytosis. We present the case report of a male adult patient, who had recently acquired a ground hedgehog, who presented in his hand a ringworm lesion incognito and a Majocchi granuloma. The etiological agent was identified as Trichophyton erinacei by mycological culture and molecular biology. The patient was treated with terbinafine oral, with excellent response...
April 2018: Revista Chilena de Infectología: órgano Oficial de la Sociedad Chilena de Infectología
https://www.readbyqxmd.com/read/29911664/revision-of-the-reciprocal-action-of-mercury-and-selenium
#4
REVIEW
Renata Kuras, Beata Janasik, Magdalena Stanislawska, Wojciech Wasowicz
Diverse forms of mercury (Hg) have various effects on animals and humans because of a variety of routes of administration. Inorganic mercury (iHg) binds to thiol groups of proteins and enzymes in one's body or is methylated by microorganisms. Organic form of Hg, contrary to the iHg, is more stable but may be demethylated to Hg2+ in the tissue of intestinal flora. Selenium (Se) also occurs in a variety of chemical forms in one's body but both of these elements behave very differently from one another. Mercury binding to selenide or Se-containing ligands is a primary molecular mechanism that reduces toxicity of Hg...
June 18, 2018: International Journal of Occupational Medicine and Environmental Health
https://www.readbyqxmd.com/read/29910778/effects-of-1-8-cineole-on-carbohydrate-metabolism-related-cell-structure-changes-of-salmonella
#5
Yangying Sun, Xiaojun Cai, Jinxuan Cao, Zhen Wu, Daodong Pan
Salmonella is gram-negative foodborne zoonotic bacteria which triggers disease in humans. Our previous studies showed 1,8-cineole possessed remarkable antimicrobial effects on foodborne zoonotic bacteria indicating it could serve as a new source of antibiotic for Salmonella . Present study elucidated the antibacterial mechanism of 1,8-cineole by analyzing serum protein expressed by Salmonella following treatment with 1,8-cineole (0.25 mg/mL, 3 h) using isobaric tags for relative and absolute quantification (iTRAQ) with two-dimensional liquid chromatography/tandem mass spectrometry (2D-LC-MS/MS)...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29910660/the-evolution-of-biosimilars-in-oncology-with-a-focus-on-trastuzumab
#6
N A Nixon, M B Hannouf, S Verma
Cancer therapy has evolved significantly with increased adoption of biologic agents ("biologics"). That evolution is especially true for her2 (human epidermal growth factor receptor-2)-positive breast cancer with the introduction of trastuzumab, a monoclonal antibody against the her2 receptor, which, in combination with chemotherapy, significantly improves survival in both metastatic and early disease. Although the efficacy of biologics is undeniable, their expense is a significant contributor to the increasing cost of cancer care...
June 2018: Current Oncology
https://www.readbyqxmd.com/read/29909453/m-6-a-rna-methylation-controls-neural-development-and-is-involved-in-human-diseases
#7
REVIEW
Kunzhao Du, Longbin Zhang, Trevor Lee, Tao Sun
RNA modifications are involved in many aspects of biological functions. N6-methyladenosine (m6 A) is one of the most important forms of RNA methylation and plays a vital role in regulating gene expression, protein translation, cell behaviors, and physiological conditions in many species, including humans. The dynamic and reversible modification of m6 A is conducted by three elements: methyltransferases ("writers"), such as methyltransferase-like protein 3 (METTL3) and METTL14; m6 A-binding proteins ("readers"), such as the YTH domain family proteins (YTHDFs) and YTH domain-containing protein 1 (YTHDC1); and demethylases ("erasers"), such as fat mass and obesity-associated protein (FTO) and AlkB homolog 5 (ALKBH5)...
June 16, 2018: Molecular Neurobiology
https://www.readbyqxmd.com/read/29908591/emerging-viruses-in-bees-from-molecules-to-ecology
#8
Dino P McMahon, Lena Wilfert, Robert J Paxton, Mark J F Brown
Emerging infectious diseases arise as a result of novel interactions between populations of hosts and pathogens, and can threaten the health and wellbeing of the entire spectrum of biodiversity. Bees and their viruses are a case in point. However, detailed knowledge of the ecological factors and evolutionary forces that drive disease emergence in bees and other host-pathogen communities is surprisingly lacking. In this review, we build on the fundamental insight that viruses evolve and adapt over timescales that overlap with host ecology...
2018: Advances in Virus Research
https://www.readbyqxmd.com/read/29908207/sumo1-sentrin-smt3-specific-peptidase-2-modulates-target-molecules-and-its-corresponding-functions
#9
REVIEW
Shan-Ling Liu, Feng-Hou Gao
Small ubiquitin-like modifier (SUMOylation) is a reversible post-translational modification, which plays important roles in numerous biological processes. SUMO could be covalently attached to target proteins in an isopeptide bond manner that occurs via a lysine ε-amino group on the target proteins and the glycine on SUMO C-terminus. This covalent binding could affect the subcellular localization and stability of target proteins. SUMO modification can be reversed by members of the Sentrin/SUMO-specific proteases (SENPs) family, which are highly evolutionarily conserved from yeast to human...
June 13, 2018: Biochimie
https://www.readbyqxmd.com/read/29908156/classifying-the-molecular-functions-of-rab-gtpases-in-membrane-trafficking-using-deep-convolutional-neural-networks
#10
Nguyen-Quoc-Khanh Le, Quang-Thai Ho, Yu-Yen Ou
Deep learning has been increasingly used to solve a number of problems with state-of-the-art performance in a wide variety of fields. In biology, deep learning can be applied to reduce feature extraction time and achieve high levels of performance. In our present work, we apply deep learning via two-dimensional convolutional neural networks and position-specific scoring matrices to classify Rab protein molecules, which are main regulators in membrane trafficking for transferring proteins and other macromolecules throughout the cell...
June 13, 2018: Analytical Biochemistry
https://www.readbyqxmd.com/read/29907771/mir-30-disrupts-senescence-and-promotes-cancer-by-targeting-both-p16-ink4a-and-dna-damage-pathways
#11
Weijun Su, Lixin Hong, Xin Xu, Shan Huang, Denise Herpai, Lisheng Li, Yingxi Xu, Lan Truong, Wen-Yuan Hu, Xiaohua Wu, Changchun Xiao, Wei Zhang, Jiahuai Han, Waldemar Debinski, Rong Xiang, Peiqing Sun
miR-30 is a microRNA frequently overexpressed in human cancers. However, the biological consequence of miR-30 overexpression in cancer has been unclear. In a genetic screen, miR-30 was found to abrogate oncogenic-induced senescence, a key tumor-suppressing mechanism that involves DNA damage responses, activation of p53 and induction of p16INK4A . In cells and mouse models, miR-30 disrupts senescence and promotes cancer by suppressing 2 targets, CHD7 and TNRC6A. We show that while CHD7 is a transcriptional coactivator essential for induction of p16INK4A in senescent cells, TNRC6A, a miRNA machinery component, is required for expression and functionality of DNA damage response RNAs (DDRNAs) that mediate DNA damage responses and p53 activation by orchestrating histone modifications, chromatin remodeling and recruitment of DNA damage factors at damaged sites...
June 15, 2018: Oncogene
https://www.readbyqxmd.com/read/29907252/opisthorchis-viverrini-draft-genome-biomedical-implications-and-future-avenues
#12
Neil D Young, Robin B Gasser
Opisthorchiasis is a neglected tropical disease of major proportion, caused by the carcinogenic, Asian liver fluke, Opisthorchis viverrini. This hepatobiliary disease is known to be associated with malignant cancer (cholangiocarcinoma, CCA) and affects millions of people in Southeast Asia. No vaccine is available, and only one drug (praziquantel) is routinely employed against the parasite. Despite technological advances, little is known about the molecular biology of the fluke itself and the disease complex that it causes in humans...
2018: Advances in Parasitology
https://www.readbyqxmd.com/read/29907131/confirmation-of-covalently-linked-structure-and-cell-death-inducing-activity-in-site-specific-chemical-conjugates-of-human-fas-ligand-extracellular-domain
#13
Michiro Muraki, Kiyonori Hirota
OBJECTIVE: In this study, we aimed to identify the structural components and to clarify the biological activity in the site-specific conjugates of human Fas ligand extracellular domain (hFasLECD) with either fluorescein moiety (FL) or chicken egg-white avidin (Avi). The conjugates were characterized by molecular-weight measurement using MALDI-TOF mass-spectrometric analysis and by cell-death inducing activity measurement against a human colorectal cancer cell line, HT-29, using MTT cell-viability assay...
June 15, 2018: BMC Research Notes
https://www.readbyqxmd.com/read/29906414/guide-rna-selection-for-crispr-cas9-transfections-in-plasmodium-falciparum
#14
Jose M Ribeiro, Meera Garriga, Nicole Potchen, Anna K Crater, Ankit Gupta, Daisuke Ito, Sanjay A Desai
CRISPR-Cas9 mediated genome editing is addressing key limitations in the transfection of malaria parasites. While this method has already simplified the needed molecular cloning and reduced the time required to generate mutants in the human pathogen Plasmodium falciparum, optimal selection of required guide RNAs and guidelines for successful transfections have not been well characterized, leading workers to use time-consuming trial and error approaches. We used a genome-wide computational approach to create a comprehensive and publicly accessible database of possible guide RNA sequences in the P...
June 12, 2018: International Journal for Parasitology
https://www.readbyqxmd.com/read/29906306/topical-silver-and-gold-nanoparticles-complexed-with-cornus-mas-suppress-inflammation-in-human-plaques-psoriasis-by-inhibiting-nf-%C3%AE%C2%BAb-activity
#15
D Crisan, K Scharffetter-Kochanek, M Crisan, S Schatz, H Hainzl, L Olenic, A Filip, L A Schneider, A Sindrilaru
New biomaterials based on nanoparticles (NPs) carrying polyphenols-rich extracts (Cornus mas) recently showed promising anti-inflammatory activity in psoriasis. We aimed to understand how topically delivered silver and gold nanoparticles complexed with Cornus mas (Ag-NPs-CM, Au-NPs-CM) modulate inflammation in psoriasis at cellular and molecular level. The impact on psoriatic inflammation was assessed in vitro on pro-inflammatory macrophages, by clinical score, high-frequency ultrasonography and immunohistology of psoriasis plaques treated with Ag-NPs-CM, Au-NPs-CM or control...
June 15, 2018: Experimental Dermatology
https://www.readbyqxmd.com/read/29905792/yeast-as-a-tool-to-identify-anti-aging-compounds
#16
Andreas Zimmermann, Sebastian Hofer, Tobias Pendl, Katharina Kainz, Frank Madeo, Didac Carmona-Gutierrez
In the search for interventions against aging and age-related diseases, biological screening platforms are indispensable tools to identify anti-aging compounds among large substance libraries. The budding yeast, Saccharomyces cerevisiae, has emerged as a powerful chemical and genetic screening platform, as it combines a rapid workflow with experimental amenability and the availability of a wide range of genetic mutant libraries. Given the amount of conserved genes and aging mechanisms between yeast and human, testing candidate anti-aging substances in yeast gene-deletion or overexpression collections, or de novo derived mutants, has proven highly successful in finding potential molecular targets...
September 1, 2018: FEMS Yeast Research
https://www.readbyqxmd.com/read/29904607/water-in-the-human-body-an-anesthesiologist-s-perspective-on-the-connection-between-physicochemical-properties-of-water-and-physiologic-relevance
#17
REVIEW
Efraín Riveros-Perez, Ricardo Riveros
The unique structure and multifaceted physicochemical properties of the water molecule, in addition to its universal presence in body compartments, make water a key player in multiple biological processes in human physiology. Since anesthesiologists deal with physiologic processes where water molecules are critical at different levels, and administer medications whose pharmacokinetics and pharmacodynamics depend on interaction with water molecules, we consider that exploration of basic science aspects related to water and its role in physiology and pharmacology is relevant to the practice of anesthesiology...
February 2018: Annals of Medicine and Surgery
https://www.readbyqxmd.com/read/29904507/simple-protocol-for-molecular-fingerprinting-of-human-oral-microbiota-samples-in-lab-classes
#18
Ana C Henriques, Paolo De Marco
DNA fingerprinting is a major tool in identifying individuals and in evidence matching. However, this technique can be difficult to reproduce in practical classes. Here, we report on distinct PCR profiles obtained when amplifying saliva DNA of a score of distinct individuals with Random Amplified Polymorphic DNA (RAPD)-PCR primer BOXA1R. The RAPD-PCR method is simple and efficient for discrimination between bacterial strains and is used in this instance to obtain personalized fingerprints of each individual's oral microbiota...
2018: Journal of Microbiology & Biology Education: JMBE
https://www.readbyqxmd.com/read/29902974/aldh1a-inhibition-sensitizes-colon-cancer-cells-to-chemotherapy
#19
Z Kozovska, A Patsalias, V Bajzik, E Durinikova, L Demkova, S Jargasova, B Smolkova, J Plava, L Kucerova, M Matuskova
BACKGROUND: Recent evidence in cancer research, developed the notion that malignant tumors consist of different subpopulations of cells, one of them, known as cancer stem cells, being attributed many important properties such as enhanced tumorigenicity, proliferation potential and profound multidrug resistance to chemotherapy. Several key stem cells markers were identified in colon cancer. In our study we focused on the aldehyde dehydrogenase type 1 (ALDH1) expression in colon cancer-derived cell lines HT-29/eGFP, HCT-116/eGFP and LS-180/eGFP, and its role in the chemoresistance and tumorigenic potential...
June 15, 2018: BMC Cancer
https://www.readbyqxmd.com/read/29902695/therapeutic-strategies-for-targeting-neurodegenerative-protein-misfolding-disorders
#20
REVIEW
Robert H Scannevin
Neurodegenerative diseases can arise from a multitude of different pathological drivers, however protein misfolding appears to be a common molecular feature central to several disorders. Protein folding, and attainment of correct secondary and tertiary structure, is essential for proper protein function. Protein misfolding gives rise to structural perturbations that can result in loss of protein function or a gain of toxic function, such as through aggregation, either of which can initiate and propagate biological responses that are deleterious to cells...
June 11, 2018: Current Opinion in Chemical Biology
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