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https://www.readbyqxmd.com/read/28645582/improved-pfastbac%C3%A2-donor-plasmid-vectors-for-higher-protein-production-using-the-bac-to-bac%C3%A2-baculovirus-expression-vector-system
#1
Hui Shang, Tyler A Garretson, C M Senthil Kumar, Robert F Dieter, Xiao-Wen Cheng
The Autographa californica multiple nucleopolyhedrovirus (AcMNPV)-based Bac-to-Bac(®) expression system consists of a bacmid and five pFastBac™ donor transfer vectors. It has been widely used for eukaryotic gene expression in insect cells to elucidate gene function in biotechnology laboratories. The pFastBac™ vectors contain a 50bp AcMNPV polyhedrin (polh) promoter and a 127bp SV40 polyadenylation (pA) signal for cloning a gene of interest into the bacmid, resulting in unsolved lower gene expression levels than the wild type (wt) AcMNPV in insect cells...
June 20, 2017: Journal of Biotechnology
https://www.readbyqxmd.com/read/28641392/alcohol-is-not-protective-against-mortality-following-traumatic-brain-injury
#2
Jennifer S Albrecht, Majid Afshar, Deborah M Stein, Gordon S Smith
Alcohol exposure results in reduced mortality following traumatic brain injury (TBI) in animal models, yet clinical studies reported conflicting results and clinical trials based on proposed mechanisms have been disappointing. Methodological issues common to many of the clinical studies may have contributed to spurious results. Our objective was to evaluate the association between blood alcohol concentration (BAC) and in-hospital mortality following TBI, overcoming methodological problems of prior studies. We conducted a retrospective cohort study on individuals treated for isolated TBI at the R Adams Cowley Shock Trauma Center between 1997-2012 (n = 1,084)...
June 21, 2017: American Journal of Epidemiology
https://www.readbyqxmd.com/read/28640892/novel-resistance-to-cydia-pomonella-granulovirus-cpgv-in-codling-moth-shows-autosomal-and-dominant-inheritance-and-confers-cross-resistance-to-different-cpgv-genome-groups
#3
Annette J Sauer, Eva Fritsch, Karin Undorf-Spahn, Petr Nguyen, Frantisek Marec, David G Heckel, Johannes A Jehle
Commercial Cydia pomonella granulovirus (CpGV) products have been successfully applied to control codling moth (CM) in organic and integrated fruit production for more than 30 years. Since 2005, resistance against the widely used isolate CpGV-M has been reported from different countries in Europe. The inheritance of this so-called type I resistance is dominant and linked to the Z chromosome. Recently, a second form (type II) of CpGV resistance in CM was reported from a field population (NRW-WE) in Germany. Type II resistance confers reduced susceptibility not only to CpGV-M but to most known CpGV isolates and it does not follow the previously described Z-linked inheritance of type I resistance...
2017: PloS One
https://www.readbyqxmd.com/read/28639827/the-effects-of-alcohol-expectancy-and-intake-on-slot-machine-gambling-behavior
#4
Dominic Sagoe, Rune Aune Mentzoni, Tony Leino, Helge Molde, Sondre Haga, Mikjel Fredericson Gjernes, Daniel Hanss, Ståle Pallesen
Background and aims Although alcohol intake and gambling often co-occur in related venues, there is conflicting evidence regarding the effects of alcohol expectancy and intake on gambling behavior. We therefore conducted an experimental investigation of the effects of alcohol expectancy and intake on slot machine gambling behavior. Methods Participants were 184 (females = 94) individuals [age range: 18-40 (mean = 21.9) years] randomized to four independent conditions differing in information/expectancy about beverage (told they received either alcohol or placebo) and beverage intake [actually ingesting low (target blood alcohol concentration [BAC] < 0...
June 22, 2017: Journal of Behavioral Addictions
https://www.readbyqxmd.com/read/28636875/effects-of-prenatal-binge-like-ethanol-exposure-and-maternal-stress-on-postnatal-morphological-development-of-hippocampal-neurons-in-rats
#5
Ewa Jakubowska-Dogru, Birsen Elibol, Ilknur Dursun, Sinan Yürüker
BACKGROUND: Alcohol is one of the most commonly used drugs of abuse negatively affecting human health and it is known as a potent teratogen responsible for fetal alcohol syndrome (FAS), which is characterized by cognitive deficits especially pronounced in juveniles but ameliorating in adults. Searching for the potential morphological correlates of these effects, in this study, we compared the course of developmental changes in the morphology of principal hippocampal neurons in fetal-alcohol (A group), intubated control (IC group), and intact control male rats (C group) over a protracted period of the first two postnatal months...
June 18, 2017: International Journal of Developmental Neuroscience
https://www.readbyqxmd.com/read/28633517/biomethanation-of-sewage-sludge-with-food-waste-leachate-via-co-digestion
#6
Jingyeong Shin, Young Beom Kim, Jong Hun Jeon, Sangki Choi, In Kyu Park, Young Mo Kim
Anaerobic mono- and co-digestion of sewage sludge (SS) and food waste leachate (FWL) were performed by assessing methane production and characterizing microbial communities. Anaerobic digestion (AD) of waste activated sludge (WAS) alone produced the lowest methane production (281 mL-CH4), but an approximately 80% increase in methane production was achieved via co-digestion of WAS and FWL (506 mL-CH4). There were less differences in the diversity of bacterial communities in anaerobic digesters, while archaeal (ARC) and bacterial (BAC) amounts reflected AD's performance...
June 21, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28633431/differential-effects-of-alcohol-on-memory-performance-in-adolescent-men-and-women-with-a-binge-drinking-history
#7
Concepción Vinader-Caerols, Andrew Talk, Adriana Montañés, Aránzazu Duque, Santiago Monleón
Aims: Binge drinking (BD) is characterized by intermittent consumption of large quantities of alcohol in short periods. This pattern of drinking is prevalent among adolescents, and has been associated with undermined learning and memory ability. This study investigates the relationships between a history of BD and the effects of acute exposure to alcohol on learning and memory performance in adolescent men and women. Methods: A high, acute dose of alcohol or control refreshment was administered to a sample of 172 adolescent undergraduate students, some of which had a history of BD and others of which had refrained from alcohol consumption...
June 17, 2017: Alcohol and Alcoholism: International Journal of the Medical Council on Alcoholism
https://www.readbyqxmd.com/read/28628988/enhanced-power-generation-and-wastewater-treatment-in-sustainable-biochar-electrodes-based-bioelectrochemical-system
#8
Binwei Wang, Zhifen Wang, Yong Jiang, Guangcai Tan, Nan Xu, Yang Xu
Corn-straw biochar (BC500 and BC900) and KOH modified biochar (BAC) were used as the electrode materials of bioelectrochemical system (BES). Compared to carbon felt (CF) electrodes BES, the maximum power density of BC500, BC900 and BAC anodes BES increased by 10.7%, 56.0% and 92.0%, and that of BC500, BC900 and BAC cathodes BES increased by 3.1, 5.2 and 4.8 times, respectively. The CF electrodes BES was optimized to decolor the AO7 simulated wastewater and 97% of AO7 was quickly degraded within 2h. When using biochar anodes, the decoloration rates were enhanced...
May 29, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28628197/vesicular-acetylcholine-transporter-vacht-overexpression-induces-major-modifications-of-striatal-cholinergic-interneuron-morphology-and-function
#9
Helena Janickova, Vania F Prado, Marco A M Prado, Salah El Mestikawy, Véronique Bernard
Striatal cholinergic interneurons (CIN) are pivotal for the regulation of the striatal network. Acetylcholine (ACh) released by CIN is centrally involved in reward behavior as well as locomotor or cognitive functions. Recently, BAC transgenic mice expressing channelrhodopsin-2 (ChR2) protein under the control of the choline acetyltransferase (ChAT) promoter (ChAT-ChR2) and displaying almost 50 extra copies of the VAChT gene were used to dissect cholinergic circuit connectivity and function using optogenetic approaches...
June 19, 2017: Journal of Neurochemistry
https://www.readbyqxmd.com/read/28625614/characterization-of-an-acquired-jumping-translocation-involving-3q13-31-qter-in-a-patient-with-de-novo-acute-monocytic-leukemia
#10
Eigil Kjeldsen
We studied an adult with de novo acute monocytic leukemia and a dismal outcome where her leukemic cells harbored an acquired rare jumping translocation (JT). We used oligo-based array CGH (oaCGH) analysis, fluorescence in situ hybridization (FISH), and 24-color karyotyping to enhance the characterization of the JT. G-banding detected a JT involving the 3q13.3-qter chromosomal segment and the recipient chromosomal regions 17p, 8q, and 15q. Each clone with JT was associated with trisomy 8. oaCGH analysis revealed an additional submicroscopic deletion in 3q13...
June 15, 2017: Experimental and Molecular Pathology
https://www.readbyqxmd.com/read/28624588/segmented-regression-analysis-of-interrupted-time-series-data-to-assess-outcomes-of-a-south-american-road-traffic-alcohol-policy-change
#11
Beatriz Nistal-Nuño
BACKGROUND: In Chile, a new law introduced in March 2012 decreased the legal blood alcohol concentration (BAC) limit for driving while impaired from 1 to 0.8 g/l and the legal BAC limit for driving under the influence of alcohol from 0.5 to 0.3 g/l. The goal is to assess the impact of this new law on mortality and morbidity outcomes in Chile. METHODS: A review of national databases in Chile was conducted from January 2003 to December 2014. Segmented regression analysis of interrupted time series was used for analyzing the data...
June 14, 2017: Public Health
https://www.readbyqxmd.com/read/28621052/10p15-3p13-duplication-inherited-from-paternal-balance-translocation-46-xy-t-5-10-q35-1-p13-identified-on-non-invasive-prenatal-testing
#12
Jin Mei, Hao Wang, Liyong Zhan
Balanced reciprocal translocations are relatively common human genetic abnormalities that involve the exchange of the terminal segments between different chromosomes and have an approximately 5-80% chance of generating an embryo with chromosomal abnormalities. Non-invasive prenatal testing (NIPT) has been increasingly used in clinical practice to detect fetal trisomies 21, 18 and 13 with a sensitivity and specificity of up to 99%. In this report, we describe a duplication on chromosome 10 and a deletion on chromosome 5 that were first detected on NIPT...
June 2017: Journal of Obstetrics and Gynaecology Research
https://www.readbyqxmd.com/read/28618354/canadian-drivers-attitudes-regarding-preventative-responses-to-driving-while-impaired-by-alcohol
#13
Ward Vanlaar, Louise Nadeau, Anna McKiernan, Marisela M Hing, Marie Claude Ouimet, Thomas G Brown
BACKGROUND: In many jurisdictions, a risk assessment following a first driving while impaired (DWI) offence is used to guide administrative decision making regarding driver relicensing. Decision error in this process has important consequences for public security on one hand, and the social and economic well being of drivers on the other. Decision theory posits that consideration of the costs and benefits of decision error is needed, and in the public health context, this should include community attitudes...
June 12, 2017: Accident; Analysis and Prevention
https://www.readbyqxmd.com/read/28614915/identification-of-an-enhancer-critical-for-the-ephirn-a5-gene-expression-in-the-posterior-region-of-the-mesencephalon
#14
Eunjeong Park, Hyuna Noh, Soochul Park
Ephrin-A5 has been implicated in the regulation of brain morphogenesis and axon pathfinding. In this study, we used bacterial homologous recombination to express a LacZ reporter in various ephrin-A5 BAC clones to identify elements that regulate ephrin-A5 gene expression during mesencephalon development. We found that there is mesencephalonspecific enhancer activity localized to a specific +25.0 kb to +30.5 kb genomic region in the first intron of ephrin-A5. Further comparative genomic analysis indicated that two evolutionary conserved regions, ECR1 and ECR2, were present within this 5...
June 13, 2017: Molecules and Cells
https://www.readbyqxmd.com/read/28606213/is-emotional-intelligence-impaired-in-unaffected-siblings-of-patients-with-schizophrenia
#15
Beatrice Frajo-Apor, Georg Kemmler, Silvia Pardeller, Markus Huber, Christian Macina, Anna-Sophia Welte, Alex Hofer
OBJECTIVES: Social cognitive deficits have been discussed to be endophenotypes for schizophrenia and other serious mental illnesses. The current study aimed to assess emotional intelligence (EI) in unaffected siblings of schizophrenia patients to investigate its potential role as endophenotype for schizophrenia. METHODS: EI was measured in 56 schizophrenia patients, 57 unaffected siblings, and 127 healthy control subjects by using the Mayer-Salovey-Caruso Emotional Intelligence Test (MSCEIT)...
June 13, 2017: Journal of the International Neuropsychological Society: JINS
https://www.readbyqxmd.com/read/28606110/a-c9orf72-bac-mouse-model-recapitulates-key-epigenetic-perturbations-of-als-ftd
#16
Rustam Esanov, Gabriela Toro Cabrera, Nadja S Andrade, Tania F Gendron, Robert H Brown, Michael Benatar, Claes Wahlestedt, Christian Mueller, Zane Zeier
BACKGROUND: Amyotrophic Lateral Sclerosis (ALS) is a fatal and progressive neurodegenerative disorder with identified genetic causes representing a significant minority of all cases. A GGGGCC hexanucleotide repeat expansion (HRE) mutation within the C9ORF72 gene has recently been identified as the most frequent known cause of ALS. The expansion leads to partial heterochromatinization of the locus, yet mutant RNAs and dipeptide repeat proteins (DPRs) are still produced in sufficient quantities to confer neurotoxicity...
June 12, 2017: Molecular Neurodegeneration
https://www.readbyqxmd.com/read/28605266/estimated-blood-alcohol-concentrations-achieved-by-consuming-supersized-alcopops
#17
Matthew E Rossheim, Dennis L Thombs
BACKGROUND: Producers of supersized alcopops have ignored requests from a number of state attorneys general to reduce the alcohol concentration in these products. To the contrary, new flavor options have since been released that contain even greater alcohol content so that some alcopop products now contain 5.5 standard alcoholic drinks in a single-serving can. Though alcohol content of supersized alcopops has risen, little attention has been paid to the blood alcohol concentration (BAC) level consumers can expect to achieve from drinking these products...
June 12, 2017: American Journal of Drug and Alcohol Abuse
https://www.readbyqxmd.com/read/28605058/cardiovascular-pharmacogenomics-and-cognitive-function-in-patients-with-schizophrenia
#18
Kristen M Ward, A Zarina Kraal, Stephanie A Flowers, Vicki L Ellingrod
STUDY OBJECTIVE: To examine the impact of multiple risk alleles for cognitive dysfunction and cardiovascular disease risk on cognitive function, and to determine if these relationships varied by cognitive reserve (CR) or concomitant medication use in patients with schizophrenia. DESIGN: Cross-sectional study. SETTING: Ambulatory mental health centers. PATIENTS: A total of 122 adults with a schizophrenia spectrum diagnosis who were maintained on a stable antipsychotic regimen for at least 6 months prior to study enrollment; patients were divided into three CR groups based on years of formal education: no high school completion or equivalent (low-education group [18 patients]); completion of high school or equivalent (moderate-education group [36 patients]; or any degree of post-high school education (high-education group [68 patients])...
June 12, 2017: Pharmacotherapy
https://www.readbyqxmd.com/read/28604949/-the-value-of-a-novel-prenatal-diagnosis-model-with-combination-of-karyotyping-and-bacs-on-beads-tm-technique
#19
Kai Mou, Yi Liu, Xin Wei
OBJECTIVE: To explore the value of a novel prenatal diagnosis model using combined chromosomal karyotyping and BACs-on-Beads(TM)(BoBs), a newly-developed technique. METHODS: 1048 single pregnancy pregnant women with various diagnostic indications were performed amniocentesis for prenatal diagnosis with karyotyping and BoBs simultaneously. RESULTS: Among 1047 successfully cultured specimens, 50 chromosomal abnormalities were identified with BoBs, including 43 common chromosomal trisomies, 3 chimeric chromosomes and 4 structural abnormalities, of which 3 microdeletions/microduplications were not detected with karyotyping...
June 10, 2017: Zhonghua Yi Xue Yi Chuan Xue za Zhi, Zhonghua Yixue Yichuanxue Zazhi, Chinese Journal of Medical Genetics
https://www.readbyqxmd.com/read/28602954/a-versatile-genetic-tool-to-study-midline-glia-function-in-the-drosophila-cns
#20
Swati Banerjee, E Mino, Elizabeth S Fisher, Manzoor A Bhat
Neuron-glial interactions are crucial for growth, guidance and ensheathment of axons across species. In the Drosophila CNS midline, neuron-glial interactions underlie ensheathment of commissural axons by midline glial (MG) cells in a manner similar to mammalian oligodendrocytes. Although there has been some advance in the study of neuron-glial interactions and ensheathment of axons in the CNS midline, key aspects of axonal ensheathment are still not fully understood. One of the limitations has been the unavailability of MG membrane markers that could highlight the glial processes wrapping the axons...
June 8, 2017: Developmental Biology
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