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https://www.readbyqxmd.com/read/28108144/postmortem-in-vitro-ethanol-production-it-could-be-more-common-than-we-think
#1
Maria José Quintas, Pedro Costa, Paula Melo, André Castro, João Miguel Franco, Helena M Teixeira
The blood alcohol concentration (BAC) is the most frequent determination in a Forensic Toxicology Laboratory. Despite its apparent simplicity, the results interpretation can be complex and always have relevant social and legal implications, particularly in postmortem analysis. In the present report we describe the case of a 55-year-old male with an apparent natural death by myocardial infarction, whose initial BAC was 0.18g/L but, in repeated determinations prompted by discrepancies observed in the first two, it rapidly increased to 0...
January 5, 2017: Forensic Science International
https://www.readbyqxmd.com/read/28107903/ion-mobility-spectrometry-as-a-fast-analytical-tool-in-benzalkonium-chloride-homologs-determination
#2
D Gallart-Mateu, S Armenta, F A Esteve-Turrillas, M de la Guardia
A novel procedure is proposed for the determination by ion mobility spectrometry (IMS) of C12, C14 and C16 benzalkonium chloride (BAC) homologs. The proposed method requires minimum sample treatment and the measurement was made in less than one minute. A high sensitivity was obtained for BAC determination by IMS with limit of detection values from 37 to 69µgL(-1). Accuracy of the proposed methodology was evaluated through the analysis of aqueous and alcoholic samples spiked with BAC at concentration levels from 0...
March 1, 2017: Talanta
https://www.readbyqxmd.com/read/28107820/evidence-against-a-role-for-jaagsiekte-sheep-retrovirus-in-human-lung-cancer
#3
A Dusty Miller, Marcelo De Las Heras, Jingyou Yu, Fushun Zhang, Shan-Lu Liu, Andrew E Vaughan, Thomas L Vaughan, Raul Rosadio, Stefano Rocca, Giuseppe Palmieri, James J Goedert, Junya Fujimoto, Ignacio I Wistuba
BACKGROUND: Jaagsiekte sheep retrovirus (JSRV) causes a contagious lung cancer in sheep and goats that can be transmitted by aerosols produced by infected animals. Virus entry into cells is initiated by binding of the viral envelope (Env) protein to a specific cell-surface receptor, Hyal2. Unlike almost all other retroviruses, the JSRV Env protein is also a potent oncoprotein and is responsible for lung cancer in animals. Of concern, Hyal2 is a functional receptor for JSRV in humans. RESULTS: We show here that JSRV is fully capable of infecting human cells, as measured by its reverse transcription and persistence in the DNA of cultured human cells...
January 20, 2017: Retrovirology
https://www.readbyqxmd.com/read/28107513/the-zebrafish-anillin-egfp-reporter-marks-late-dividing-retinal-precursors-and-stem-cells-entering-neuronal-lineages
#4
Meret Cepero Malo, Anne-Laure Duchemin, Luca Guglielmi, Eva Patzel, Saadettin Sel, Gerd U Auffarth, Matthias Carl, Lucia Poggi
Monitoring cycling behaviours of stem and somatic cells in the living animal is a powerful tool to better understand tissue development and homeostasis. The tg(anillin:anillin-eGFP) transgenic line carries the full-length zebrafish F-actin binding protein Anillin fused to eGFP from a bacterial artificial chromosome (BAC) containing Anillin cis-regulatory sequences. Here we report the suitability of the Anillin-eGFP reporter as a direct indicator of cycling cells in the late embryonic and post-embryonic retina...
2017: PloS One
https://www.readbyqxmd.com/read/28098790/synthesis-structure-and-antimicrobial-properties-of-novel-benzalkonium-chloride-analogues-with-pyridine-rings
#5
Bogumił Brycki, Izabela Małecka, Anna Koziróg, Anna Otlewska
Quaternary ammonium compounds (QACs) are a group of compounds of great economic significance. They are widely used as emulsifiers, detergents, solubilizers and corrosion inhibitors in household and industrial products. Due to their excellent antimicrobial activity QACs have also gained a special meaning as antimicrobials in hospitals, agriculture and the food industry. The main representatives of the microbiocidal QACs are the benzalkonium chlorides (BACs), which exhibit biocidal activity against most bacteria, fungi, algae and some viruses...
January 13, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28095770/the-unique-genomic-landscape-surrounding-the-epsps-gene-in-glyphosate-resistant-amaranthus-palmeri-a-repetitive-path-to-resistance
#6
William T Molin, Alice A Wright, Amy Lawton-Rauh, Christopher A Saski
BACKGROUND: The expanding number and global distributions of herbicide resistant weedy species threaten food, fuel, fiber and bioproduct sustainability and agroecosystem longevity. Amongst the most competitive weeds, Amaranthus palmeri S. Wats has rapidly evolved resistance to glyphosate primarily through massive amplification and insertion of the 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) gene across the genome. Increased EPSPS gene copy numbers results in higher titers of the EPSPS enzyme, the target of glyphosate, and confers resistance to glyphosate treatment...
January 17, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28095034/self-reported-driving-under-the-influence-of-alcohol-and-cannabis-among-ontario-students-associations-with-graduated-licensing-risk-taking-and-substance-abuse
#7
Steven Cook, Danielle Shank, Tara Bruno, Nigel E Turner, Robert E Mann
OBJECTIVE: This paper describes the patterns of self-reported driving under the influence of alcohol (DUIA) and driving under the influence of cannabis (DUIC) among licenced Ontario students in 2009, and examines their associations with graduated licencing, risk taking, and substance use problems for understanding DUIA and DUIC behaviours. Ontario's graduated licencing system requires new drivers to hold a G1 license for a minimum of 8 months, and a G2 licence for a minimum of 12 months before a full and unrestricted G license can be obtained...
January 17, 2017: Traffic Injury Prevention
https://www.readbyqxmd.com/read/28092936/the-effect-of-ozonation-and-biological-activated-carbon-treatment-of-wastewater-effluents-on-formation-of-n-nitrosamines-and-halogenated-disinfection-byproducts
#8
Yi-Hsueh Chuang, William A Mitch
Ozonation followed by biological activated carbon (O3/BAC) is being considered as a key component of reverse osmosis-free advanced treatment trains for potable wastewater reuse. Using a laboratory-scale O3/BAC system treating two nitrified wastewater effluents, this study characterized the effect of different ozone dosages (0-1.0 mg O3/mg dissolved organic carbon) and BAC empty bed contact times (EBCT; 15-60 minutes) on the formation after chlorination or chloramination of 35 regulated and unregulated halogenated disinfection byproducts (DBPs), 8 N-nitrosamines, and bromate...
January 17, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/28092034/expression-and-purification-of-sh2-domains-using-baculovirus-expression-system
#9
Mari Ogiue-Ikeda, Kazuya Machida
Recombinant proteins expressed in bacteria are sometimes insoluble, aggregated, and incorrectly folded. For those Src homology 2 (SH2) domains that are insoluble in bacteria, baculovirus-insect cell expression systems can be an alternative to produce soluble and functionally active proteins. We describe a protocol for cloning and purification of GST-tagged SH2 domains using the Bac-to-Bac baculovirus expression system.
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28087103/identifying-methanogen-community-structures-and-their-correlations-with-performance-parameters-in-four-full-scale-anaerobic-sludge-digesters
#10
Taewoan Koo, Seung Gu Shin, Joonyeob Lee, Gyuseong Han, Woong Kim, Kyungjin Cho, Seokhwan Hwang
Four full-scale mesophilic anaerobic digesters treating waste sludge were monitored to characterize methanogen communities and their relationship with process parameters. The performance of the four digesters were dissimilar with the average chemical oxygen demand removal efficiencies between 24 and 45% and differing pH. Real-time quantitative PCR showed that archaeal 16S rRNA gene concentration ([ARC]) and, more pronouncedly, its ratio to bacterial counterpart ([ARC]/[BAC]) correlated positively with the performance parameters, including the lipid removal efficiency...
January 4, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28078788/violence-involvement-among-nightlife-patrons-the-relative-role-of-demographics-and-substance-use
#11
Trond Nordfjaern
The nightlife setting is a risk context for violence involvement that ultimately may cause severe injuries and fatalities. Few studies have examined associations between alcohol and illicit substance use with physical violence involvement among nightlife patrons. The aim of the current study was to investigate the relative role of demographics and substance use characteristics for nightlife violence involvement among Norwegian nightlife patrons. A cross-sectional self-completion survey was conducted outside 12 licensed premises in Oslo (n = 1099, response rate = 76%) and each respondent's BAC level was measured by a breathalyzer and registered on the questionnaire...
January 12, 2017: Aggressive Behavior
https://www.readbyqxmd.com/read/28074752/removal-of-17%C3%AE-estradiol-in-a-biological-active-carbon-reactor-with-acetic-acid-and-humic-acid
#12
Zhongtian Li
The objective of this study is to characterize the removal of 17β-estradiol (E2) and the microbial community of a biologically active carbon (BAC) reactor under acetic acid or humic acid as the primary carbon source. Influent E2 concentration was maintained at 20 μg/L. Higher than 99 % removal of E2 was achieved by the BAC reactor. E2 concentration increased from below detection limit (<5.8 ng/L) to 48±8 ng/L after switching the primary carbon source from acetic acid to humic acid in the reactor influent...
January 6, 2017: Water Environment Research: a Research Publication of the Water Environment Federation
https://www.readbyqxmd.com/read/28070617/a-pooled-analysis-of-on-the-road-highway-driving-studies-in-actual-traffic-measuring-standard-deviation-of-lateral-position-i-e-weaving-while-driving-at-a-blood-alcohol-concentration-of-0-5%C3%A2-g-l
#13
S Jongen, A Vermeeren, N N J J M van der Sluiszen, M B Schumacher, E L Theunissen, K P C Kuypers, E F P M Vuurman, J G Ramaekers
INTRODUCTION: The on-the-road highway driving test is generally regarded as a gold standard for assessing drug-induced driving impairment. The primary outcome measure is the standard deviation of lateral position (SDLP), a measure of road tracking error or "weaving". The test has been calibrated for incremental doses of alcohol almost 30 years ago in order to define the impact of drug-induced impairment in terms of blood alcohol concentration (BAC) equivalents. Drug-induced changes in SDLP exceeding 2...
January 9, 2017: Psychopharmacology
https://www.readbyqxmd.com/read/28068535/comparison-of-micropollutants-removal-performance-between-pre-ozonation-and-post-ozonation-using-a-pilot-study
#14
Kai Yang, Jianwei Yu, Qingyuan Guo, Chunmiao Wang, Min Yang, Yu Zhang, Ping Xia, Dong Zhang, Zhiyong Yu
Despite the strong oxidizing ability of ozone, pre-ozonation has seldom been employed for the purpose of micropollutant removal in drinking water utilities. In this paper, the possibility of using pre-ozonation instead of post-ozonation for the removal of micropollutants was explored because of the lower risk of forming carcinogenic bromate. A 1.0 m(3)/h pilot system was utilized to compare the efficacy of pre- and post-ozonation in the removal of bulk organic pollutants as well as micropollutants, including typical odor-causing compounds, pharmaceuticals, and typical pesticides, from one source water (Huangpu River) characterized by the occurrence of various micropollutants...
December 27, 2016: Water Research
https://www.readbyqxmd.com/read/28065516/ignition-interlock-laws-effects-on-fatal-motor-vehicle-crashes-1982-2013
#15
Emma E McGinty, Gregory Tung, Juliana Shulman-Laniel, Rose Hardy, Lainie Rutkow, Shannon Frattaroli, Jon S Vernick
INTRODUCTION: Alcohol-involved motor vehicle crashes are a major cause of preventable mortality in the U.S., leading to more than 10,000 fatalities in 2013. Ignition interlocks, or alcohol-sensing devices connected to a vehicle's ignition to prevent it from starting if a driver has a predetermined blood alcohol content (BAC) level, are a promising avenue for preventing alcohol-involved driving. This study sought to assess the effects of laws requiring ignition interlocks for some or all drunk driving offenders on alcohol-involved fatal crashes...
December 27, 2016: American Journal of Preventive Medicine
https://www.readbyqxmd.com/read/28063820/assessment-of-ocular-discomfort-caused-by-5-shampoos-using-the-slug-mucosal-irritation-test
#16
Jean-Yanique Petit, Vanessa Doré, Geneviève Marignac, Sébastien Perrot
Assessment of ocular discomfort caused by veterinary care products is less legitimately regulated than that caused by human care products. The Slug Mucosal Irritation (SMI) assay was adapted to evaluate canine hygiene shampoos to predict ocular discomfort. Experiments were performed using four commercial canine shampoos, a baby care product, and two controls (ArtTear® and BAC1%). Groups of 3 slugs were tested with 5% dilution of the 7 test substances. The negative control (ArtTear®) was the best tolerated...
January 4, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/28054379/to-wait-or-not-to-wait-improving-results-when-interviewing-intoxicated-witnesses-to-violence
#17
Malin Hildebrand Karlén, Emma Roos Af Hjelmsäter, Claudia Fahlke, Pär Anders Granhag, Anna Söderpalm-Gordh
Witnesses to violent crimes are often alcohol intoxicated, but few studies have investigated the impact of alcohol on witness reports. This study investigated how alcohol intoxication and time of interview affected reports of intimate partner violence (IPV). One hundred thirty six healthy men (N = 66) and women (N = 70) were randomized to an alcohol group (0.8g/kg for men, 0.75g/kg for women) (N = 70) or control group (N = 66), given juice. Participants consumed drinks in a laboratory setting before they witnessed an IPV scenario...
February 2017: Scandinavian Journal of Psychology
https://www.readbyqxmd.com/read/28044383/alcohol-use-among-fatally-injured-victims-in-s%C3%A3-o-paulo-brazil-bridging-the-gap-between-research-and-health-services-in-developing-countries
#18
Gabriel Andreuccetti, Vilma Leyton, Nikolas P Lemos, Ivan Dieb Miziara, Yu Ye, Juliana Takitane, Daniel Romero Munoz, Arthur L Reingold, Cheryl J Cherpitel, Heraclito Barbosa de Carvalho
BACKGROUND AND AIMS: Most studies reporting alcohol use among fatally injured victims are subject to bias, particularly those related to sample selection and to absence of injury context data. We developed a research method to estimate the prevalence of alcohol consumption and test correlates of alcohol use prior to fatal injuries. DESIGN, SETTING AND PARTICIPANTS: Cross-sectional study based on a probability sample of fatally injured adult victims (n = 365) autopsied in São Paulo, Brazil...
November 6, 2016: Addiction
https://www.readbyqxmd.com/read/28035713/evaluation-of-the-effectiveness-of-the-potent-bis-quaternary-ammonium-compound-4-4-%C3%AE-%C3%AF-hexametylenedithio-bis-1-octylpyridinium-bromide-4dtbp-6-8-on-pseudomonas-aeruginosa
#19
Keiji Murakami, Hiromichi Yumoto, Ayu Murakami, Takashi Amoh, Darija Viducic, Katsuhiko Hirota, Atsushi Tabata, Hideaki Nagamune, Hiroki Kourai, Takashi Matsuo, Yoichiro Miyake
AIMS: Quaternary ammonium compounds (QACs) including benzalkonium chloride (BAC) and cetylpyridinium chloride (CPC) are cationic surfactants and have been used widely as general disinfectants in the medical field because of their strong antibacterial effects and low cytotoxicity to human cells. 4,4'-(α,ω-hexametylenedithio) bis (1-octylpyridinium bromide) (4DTBP-6,8) is one of the potent bis-QACs synthesized to improve the antimicrobial activities of mono-QACs such as BAC. This study aimed to assess the effectiveness of 4DTBP-6,8 against Pseudomonas aeruginosa, a prevalent hospital pathogen...
December 30, 2016: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28034278/proceeding-of-the-8th-alcohol-hangover-research-group-meeting
#20
Marlou Mackus, Sally Adams, Amir Barzilay, Sarah Benson, Lauren Blau, Jacqueline Iversen, Sean J Johnson, Ali Keshavarzian, Andrew Scholey, Gordon S Smith, Constantine Trela, Vatsalya Vatsalya, Joris C Verster
Alcohol hangover is one of the most commonly experienced consequences of alcohol consumption. An alcohol hangover develops as the blood alcohol concentrations (BAC) approaches zero, and is characterized by a general feeling of misery. More insight into the pathology of an alcohol hangover needs to be gained, in order to enhance the understanding of the area, and as a potential contribution to the innovation of a preventative or hangover curing treatment. The Alcohol Hangover Research Group (AHRG) was founded to support the area of alcohol hangover...
December 29, 2016: Current Drug Abuse Reviews
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