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https://www.readbyqxmd.com/read/28919972/cytogenetic-maps-of-homoeologous-chromosomes-a-h01-and-d-h01-and-their-integration-with-the-genome-assembly-in-gossypium-hirsutum
#1
Yuling Liu, Zhen Liu, Renhai Peng, Yuhong Wang, Zhongli Zhou, Xiaoyan Cai, Xingxing Wang, Zhenmei Zhang, Kunbo Wang, Fang Liu
Cytogenetic maps of Gossypium hirsutum (Linnaeus, 1753) homoeologous chromosomes Ah01 and Dh01 were constructed by fluorescence in situ hybridization (FISH), using eleven homoeologous-chromosomes-shared bacterial artificial chromosomes (BACs) clones and one chromosome-specific BAC clone respectively. We compared the cytogenetic maps with the genetic linkage and draft genome assembly maps based on a standardized map unit, relative map position (RMP), which allowed a global view of the relationship of genetic and physical distances along each chromosome, and assembly quality of the draft genome assembly map...
2017: Comparative Cytogenetics
https://www.readbyqxmd.com/read/28912439/association-between-cognitive-deficits-and-suicidal-ideation-in-patients-with-major-depressive-disorder
#2
Shenghong Pu, Shiori Setoyama, Takamasa Noda
The role of cognitive function in suicidal ideation in patients with major depressive disorder (MDD) has not been adequately explored. This research sought to measure the relationship between suicidal ideation and cognitive function. Therefore, in this study, the association between cognitive function and suicidal ideation in patients with MDD was assessed. Cognitive function was evaluated in 233 patients with MDD using the Japanese version of the Brief Assessment of Cognition in Schizophrenia (BACS). Suicidal ideation was assessed using item 3 of the Hamilton Depression Rating Scale...
September 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28912069/generation-of-a-novel-human-cytomegalovirus-bacterial-artificial-chromosome-tailored-for-transduction-of-exogenous-sequences
#3
William L Close, Amit Bhandari, Marwa Hojeij, Philip E Pellett
The study of herpesviruses, including human cytomegalovirus (HCMV), is complicated by viral genome complexity and inefficient methods for genetic manipulation in tissue culture. Reverse genetics of herpesviruses has been facilitated by propagating their genomes in E. coli as bacterial artificial chromosomes (BACs), which enables complex and precise genetic manipulation using bacterial recombinational systems. Internal capsid volume imposes a strict limit on the length of genome that can be packaged efficiently...
September 11, 2017: Virus Research
https://www.readbyqxmd.com/read/28906557/the-unusual-s-locus-of-leavenworthia-is-composed-of-two-sets-of-paralogous-loci
#4
Sier-Ching Chantha, Adam C Herman, Vincent Castric, Xavier Vekemans, William Marande, Daniel J Schoen
The Leavenworthia self-incompatibility locus (S locus) consists of paralogs (Lal2, SCRL) of the canonical Brassicaceae S locus genes (SRK, SCR), and is situated in a genomic position that differs from the ancestral one in the Brassicaceae. Unexpectedly, in a small number of Leavenworthia alabamica plants examined, sequences closely resembling exon 1 of SRK have been found, but the function of these has remained unclear. BAC cloning and expression analyses were employed to characterize these SRK-like sequences...
September 14, 2017: New Phytologist
https://www.readbyqxmd.com/read/28904201/heterogeneity-of-the-epstein-barr-virus-major-internal-repeat-reveals-evolutionary-mechanisms-of-ebv-and-a-functional-defect-in-the-prototype-ebv-strain-b95-8
#5
Mohammed Ba Abdullah, Richard Palermo, Anne Palser, Nicholas E Grayson, Paul Kellam, Samantha Correia, Agnieszka Szymula, Rob White
Epstein-Barr virus (EBV) is a ubiquitous pathogen of humans that can cause several types of lymphoma and carcinoma. Like other herpesviruses, EBV has diversified both through co-evolution with its host, and genetic exchange between virus strains. Sequence analysis of the EBV genome is unusually challenging, because of the large number and length of repeat regions within the virus. Here we describe the sequence assembly and analysis of the large internal repeat of EBV (IR1 or BamW repeats) from over 70 strains...
September 13, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28904200/epstein-barr-virus-bkrf4-gene-product-is-required-for-efficient-progeny-production
#6
H M Abdullah Al Masud, Takahiro Watanabe, Masahiro Yoshida, Yoshitaka Sato, Fumi Goshima, Hiroshi Kimura, Takayuki Murata
Epstein-Barr virus (EBV), a member of human gammaherpesvirus, infects mainly B cells. EBV has two alternative life cycles, latent and lytic, and is reactivated occasionally from the latent stage to the lytic cycle. To combat EBV-associated disorders, understanding the molecular mechanisms of the EBV lytic replication cycle is also important. Here, we focused on an EBV lytic gene, BKRF4. Using our anti-BKRF4 antibody, we revealed that the BKRF4 gene product is expressed during the lytic cycle with late kinetics...
September 13, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28894263/the-parathyroid-hormone-regulates-skin-tumour-susceptibility-in-mice
#7
Kazuhiro Okumura, Megumi Saito, Yasuhiro Yoshizawa, Haruka Munakata, Eriko Isogai, Ikuo Miura, Shigeharu Wakana, Midori Yamaguchi, Hiroshi Shitara, Choji Taya, Andrew C Karaplis, Ryo Kominami, Yuichi Wakabayashi
Using a forward genetics approach to map loci in a mouse skin cancer model, we previously identified a genetic locus, Skin tumour modifier of MSM 1 (Stmm1) on chromosome 7, conferring strong tumour resistance. Sub-congenic mapping localized Parathyroid hormone (Pth) in Stmm1b. Here, we report that serum intact-PTH (iPTH) and a genetic polymorphism in Pth are important for skin tumour resistance. We identified higher iPTH levels in sera from cancer-resistant MSM/Ms mice compared with susceptible FVB/NJ mice...
September 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28890324/direct-head-to-head-evaluation-of-recombinant-adeno-associated-viral-vectors-manufactured-in-human-versus-insect-cells
#8
Oleksandr Kondratov, Damien Marsic, Sean M Crosson, Hector R Mendez-Gomez, Oleksandr Moskalenko, Mario Mietzsch, Regine Heilbronn, Jonathan R Allison, Kari B Green, Mavis Agbandje-McKenna, Sergei Zolotukhin
The major drawback of the Baculovirus/Sf9 system for recombinant adeno-associated viral (rAAV) manufacturing is that most of the Bac-derived rAAV vector serotypes, with few exceptions, demonstrate altered capsid compositions and lower biological potencies. Here, we describe a new insect cell-based production platform utilizing attenuated Kozak sequence and a leaky ribosome scanning to achieve a serotype-specific modulation of AAV capsid proteins stoichiometry. By way of example, rAAV5 and rAAV9 were produced and comprehensively characterized side by side with HEK293-derived vectors...
August 10, 2017: Molecular Therapy: the Journal of the American Society of Gene Therapy
https://www.readbyqxmd.com/read/28888886/the-recombinant-ehv-1-vector-producing-cdv-hemagglutinin-as-potential-vaccine-against-canine-distemper
#9
Zihao Pan, Jin Liu, Jiale Ma, Qiuli Jin, Huochun Yao, Klaus Osterrieder
Canine distemper virus (CDV), is a pantropic agent of morbillivirus that causes fetal disease in dogs. Base on a broad host rang of CDV, the continued vaccines inoculation is unavoidable to pose gene recombination risk in vaccine virus and wild virus. The current study presents the construction of novel vectors, using equine herpesvirus type 1 (EHV-1) expressing the canine distemper virus (CDV). The recent field strain hemagglutinin protein and nucleoprotein were used for the construction of the viral vector vaccines...
September 6, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28888149/the-impact-of-alcohol-policies-on-alcohol-attributable-diseases-in-taiwan-a-population-based-study
#10
Yung-Hsiang Ying, Yung-Ching Weng, Koyin Chang
BACKGROUND: Taiwan has some of the strictest alcohol-related driving laws in the world. However, its laws continue to be toughened to reduce the ever-increasing social cost of alcohol-related harm. AIM: This study assumes that alcohol-related driving laws show a spillover effect such that behavioral changes originally meant to apply behind the wheel come to affect drinking behavior in other contexts. The effects of alcohol driving laws and taxes on alcohol-related morbidity are assessed; incidence rates of alcohol-attributable diseases (AAD) serve as our measure of morbidity...
September 4, 2017: Drug and Alcohol Dependence
https://www.readbyqxmd.com/read/28887719/protective-effects-of-carbenoxolone-an-11%C3%AE-hsd1-inhibitor-against-chemical-induced-dry-eye-syndrome
#11
Yoon-Ju Na, Kyoung-Jin Choi, Sung Bum Park, Hye-Rim Sung, Won Hoon Jung, Hee Youn Kim, Sang Dal Rhee, Ki Young Kim
Dry eye syndrome (DES) is a disorder of the eye due to tear deficiency or excessive evaporation that causes damage to the eye and is associated with discomfort and dryness. 11β-Hydroxysteroid dehydrogenase 1 (11β-HSD1) is an enzyme that converts inactive cortisone to active cortisol. Recently, 11β-HSD1 has been expressed in human and rodent eyes and has been recognized as a target of glaucoma. In this study, the therapeutic effects and underlying mechanisms of topical carbenoxolone, an 11β-HSD1 inhibitor, were investigated in benzalkonium chloride (BAC)-treated human conjunctival epithelial cells and a rat DES model...
September 8, 2017: Apoptosis: An International Journal on Programmed Cell Death
https://www.readbyqxmd.com/read/28878310/large-scale-production-of-recombinant-human-lactoferrin-from-high-expression-marker-free-transgenic-cloned-cows
#12
Ming Wang, Zhaolin Sun, Tian Yu, Fangrong Ding, Ling Li, Xi Wang, Mingbo Fu, Haiping Wang, Jinming Huang, Ning Li, Yunping Dai
Human lactoferrin (hLF) is a valuable protein for pharmaceutical products and functional foods, and worldwide demand for this protein has steadily increased. However, large-scale recombinant human lactoferrin (rhLF) production using current animal bioreactor techniques is limited by the low expression of foreign proteins, the use of antibiotic resistance genes and the down-regulation of endogenous milk proteins. Here, we generated a herd of marker-free, hLF bacterial artificial chromosome (BAC) transgenic cloned cows, as confirmed by Polymerase chain reaction, Southern blot and Western blot analyses...
September 6, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28876981/genetic-and-structural-analyses-of-rrnpp-intercellular-peptide-signaling-of-gram-positive-bacteria
#13
Matthew B Neiditch, Glenn C Capodagli, Gerd Prehna, Michael J Federle
Bacteria use diffusible chemical messengers, termed pheromones, to coordinate gene expression and behavior among cells in a community by a process known as quorum sensing. Pheromones of many gram-positive bac, such as Bacillus and Streptococcus, are small, linear peptides secreted from cells and subsequently detected by sensory receptors such as those belonging to the large family ofRRNPP proteins. These proteins are cytoplasmic pheromone receptors sharing a structurally similar pheromone-binding domain that functions allosterically to regulate receptor activity...
September 6, 2017: Annual Review of Genetics
https://www.readbyqxmd.com/read/28875148/long-term-bond-strength-of-two-benzalkonium-chloride-modified-adhesive-systems-to-eroded-dentin
#14
Simon Flury, Adrian Lussi, Anne Peutzfeldt
This study investigated the effect of benzalkonium chloride (BAC) modification of two adhesive systems on long-term bond strength to normal and artificially eroded dentin. A total of 128 extracted human molars were sectioned and the buccal and oral surfaces of each molar were ground until the dentin. One half was left untreated (normal dentin) while the other half underwent artificial erosion. Resin composite was bonded to the buccal or oral surface following treatment with Adper Scotchbond 1XT or OptiBond FL without or with 1% BAC incorporation...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28871864/quantifying-the-combined-effects-of-pronase-and-benzalkonium-chloride-in-removing-late-stage-listeria-monocytogenes-escherichia-coli-dual-species-biofilms
#15
Pedro Rodríguez-López, Carmen H Puga, Belén Orgaz, Marta L Cabo
This work presents the assessment of the effectivity of a pronase (PRN)-benzalkonium chloride (BAC) sequential treatment in removing Listeria monocytogenes-Escherichia coli dual-species biofilms grown on stainless steel (SS) using fluorescence microscopy and plate count assays. The effects of PRN-BAC on the occupied area (OA) by undamaged cells in 168 h dual-species samples were determined using a first-order factorial design. Empirical equations significantly (r(2) = 0.927) described a negative individual effect of BAC and a negative interactive effect of PRN-BAC achieving OA reductions up to 46%...
September 2017: Biofouling
https://www.readbyqxmd.com/read/28865677/berkeleyacetal-c-a-meroterpenoid-isolated-from-the-fungus-penicilium-purpurogenum-mhz-111-exerts-anti-inflammatory-effects-via-inhibiting-nf-%C3%AE%C2%BAb-erk1-2-and-irf3-signaling-pathways
#16
Shan-Shan Li, Jun Li, Jing Sun, Ran Guo, Lan-Zhi Yu, Yun-Fang Zhao, Zhi-Xiang Zhu, Peng-Fei Tu
Berkeleyacetal C (BAC), a meroterpenoid compound, was isolated from the fungus Penicillium purpurogenum MHZ 111 and showed favorable activity of inhibiting nitrogen oxide (NO) production of macrophages stimulated by lipopolysaccharide (LPS) in our preliminary screening. In order to develop novel therapeutic drug for acute and chronic inflammatory diseases, the anti-inflammatory activity and underlying mechanisms of BAC were investigated in macrophages and neutrophils. The results showed that BAC significantly inhibited the expression of inducible nitric oxide synthase (iNOS) and the following NO production by macrophages...
August 30, 2017: European Journal of Pharmacology
https://www.readbyqxmd.com/read/28865483/suppression-of-cortical-seizures-by-optic-stimulation-of-the-reticular-thalamus-in-pv-mhchr2-yfp-bac-transgenic-mice
#17
Wei Jen Chang, Wei Pang Chang, Bai Chuang Shyu
Deep brain stimulation in thalamic regions has been proposed as a treatment for epilepsy. The electrical current excites thalamocortical activity which is controlled by γ-aminobutyric acid (GABA)ergic interneurons in the reticular thalamic nucleus (nRT). Previous studies showed that enhancing GABAergic inhibitory strength in the nRT reduces the duration and power of seizures, indicating that the thalamus plays an important role in modulating cortical seizures. The aim of the present study was to apply optogenetics to study the role of the nRT in modulating cortical seizures...
September 2, 2017: Molecular Brain
https://www.readbyqxmd.com/read/28861267/frequency-of-antiseptic-resistance-genes-in-clinical-staphycocci-and-enterococci-isolates-in-turkey
#18
Seyda Ignak, Yasar Nakipoglu, Bulent Gurler
BACKGROUND: Disinfectants and antiseptics are biocides widely used in hospitals to prevent spread of pathogens. It has been reported that antiseptic resistance genes, qac's, caused tolerance to a variety of biocidal agents, such as benzalkonium chloride (BAC) and chlorhexidine digluconate (CHDG) in Staphylococcus spp. isolates. We aimed to search the frequency of antiseptic resistance genes in clinical Staphylococcus spp. and Enterococcus spp. isolates to investigate the possible association with antiseptic tolerance and antibiotic resistance...
2017: Antimicrobial Resistance and Infection Control
https://www.readbyqxmd.com/read/28860974/striatal-distribution-and-cytoarchitecture-of-dopamine-receptor-subtype-1-and-2-evidence-from-double-labeling-transgenic-mice
#19
Keke Ren, Baolin Guo, Chunqiu Dai, Han Yao, Tangna Sun, Xia Liu, Zhantao Bai, Wenting Wang, Shengxi Wu
As the main input nucleus of the basal ganglion, the striatum executes different functions, including motivation, reward and attention. The functions of the striatum highly rely on its subregions that receive projections from various cortical areas and the distribution of striatonigral neurons that express D1 dopamine (DA) receptors (or D1 medium-sized spiny neurons, D1 MSNs) and striatopallidal neurons that express D2 DA receptors (or D2 MSNs). Using bacterial artificial chromosome (BAC) transgenic mice, several studies have recently been performed on the spatial distribution of D1 and D2 MSNs...
2017: Frontiers in Neural Circuits
https://www.readbyqxmd.com/read/28860474/field-free-manipulation-of-magnetization-alignments-in-a-fe-gaas-gamnas-multilayer-by-spin-orbit-induced-magnetic-fields
#20
Sangyeop Lee, Taehee Yoo, Seul-Ki Bac, Seonghoon Choi, Hakjoon Lee, Sanghoon Lee, Xinyu Liu, Margaret Dobrowolska, Jacek K Furdyna
We investigate the process of selectively manipulating the magnetization alignment in magnetic layers in the Fe/GaAs/GaMnAs structure by current-induced spin-orbit (SO) magnetic field. The presence of such fields manifests itself through the hysteretic behavior of planar Hall resistance observed for two opposite currents as the magnetization in the structure switches directions. In the case of the Fe/GaAs/GaMnAs multilayer, hystereses are clearly observed when the magnetization switches direction in the GaMnAs layer, but are negligible when magnetization transitions occur in Fe...
August 31, 2017: Scientific Reports
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