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https://www.readbyqxmd.com/read/29160960/risk-of-past-year-injury-related-to-hours-of-exposure-to-an-elevated-bac-and-average-monthly-alcohol-volume-data-from-four-national-alcohol-surveys-2000-2015
#1
Cheryl J Cherpitel, Yu Ye, William C Kerr
BACKGROUND: While a strong association exists between alcohol and injury in emergency department (ED) studies, these studies are not representative of the general population. OBJECTIVE: The association of injury with the number of hours of exposure to a BAC ≥ 0.05 and average monthly volume in drinks, both based on self-report of quantity and frequency of drinking in the last year, in a merged sample of respondents (n=29,571) from four U.S. National Alcohol Surveys (2000-2015) is analyzed...
November 21, 2017: Alcoholism, Clinical and Experimental Research
https://www.readbyqxmd.com/read/29159149/procedure-related-complications-during-endovascular-treatment-of-intracranial-saccular-aneurysms
#2
Jae-Min Ahn, Jae-Sang Oh, Seok-Mann Yoon, Jae-Hyun Shim, Hyuk-Jin Oh, Hack-Gun Bae
Objective: We evaluate the rates and outcomes of major procedure-related complications during coiling. Materials and Methods: Between 2007 and 2015, 436 intracranial saccular aneurysms were treated. Complications are categorized as three types: intraprocedural aneurysm rupture (IAR), thromboembolism (TE), and post-procedural early rebleeding (PER). And we evaluated the risk factors of procedure related complications by multivariate analysis. Results: Complications occurred in 61 aneurysms (14%)...
September 2017: Journal of Cerebrovascular and Endovascular Neurosurgery
https://www.readbyqxmd.com/read/29158084/distinct-expression-patterns-of-flk1-and-flt1-in-the-coronary-vascular-system-during-development-and-after-myocardial-infarction
#3
Shota Kurotsu, Rina Osakabe, Mari Isomi, Fumiya Tamura, Taketaro Sadahiro, Naoto Muraoka, Hidenori Kojima, Sho Haginiwa, Hidenori Tani, Kaori Nara, Yoshiaki Kubota, Masatsugu Ema, Keiichi Fukuda, Takeshi Suzuki, Masaki Ieda
The coronary vascular system is critical for myocardial growth and cardiomyocyte survival. However, the molecular mechanism regulating coronary angiogenesis remains elusive. Vascular endothelial growth factor (VEGF) regulates angiogenesis by binding to the specific receptors Flk1 and Flt1, which results in different functions. Despite the importance of Flk1 and Flt1, their expression in the coronary vasculature remains largely unknown due to the lack of appropriate antibodies for immunostaining. Here, we analyzed multiple reporter mice including Flk1-GFP BAC transgenic (Tg), Flk1-LacZ knock-in, Flt1-DsRed BAC Tg, and Flk1-GFP/Flt1-DsRed double Tg animals to determine expression patterns in mouse hearts during cardiac growth and after myocardial infarction (MI)...
November 17, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29156284/temporal-characterization-and-statistical-analysis-of-flowback-and-produced-waters-and-their-potential-for-reuse
#4
Karl Oetjen, Kevin E Chan, Kristoffer Gulmark, Jan H Christensen, Jens Blotevogel, Thomas Borch, John R Spear, Tzahi Y Cath, Christopher P Higgins
Hydraulic fracturing (HF) has allowed for the utilization of previously unattainable shale oil and gas (O&G) resources. After HF is complete, the waters used to increase the facies' permeability return uphole as wastewaters. When these waters return to the surface, they are characterized by complex organic and inorganic chemistry, and can pose a health risk if not handled correctly. Therefore, these waters must be treated or disposed of properly. However, the variability of these waters' chemical composition over time is poorly understood and likely limits the applicability of their reuse...
November 17, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/29152755/interviewing-intoxicated-witnesses-memory-performance-in-theory-and%C3%A2-practice
#5
Malin Hildebrand Karlén
Memory encoding and recall involving complex, effortful cognitive processes are impaired by alcohol primarily due to impairment of a select few, but crucial, cortical areas. This review shows how alcohol affects some, but not all, aspects of eyewitnesses' oral free recall performance. The principal results, so far, are that: a) free recall reports by intoxicated witnesses (at the investigated BAC-levels) may contain less, but as accurate, information as reports by sober witnesses; b) immediate reports given by intoxicated witnesses may yield more information compared to reports by sober witnesses given after a one week delay; c) an immediate interview may enhance both intoxicated and sober witnesses' ability to report information in a later interview; and d) reminiscence seems to occur over repeated interviews and the new information seems to be as accurate as the previously reported information...
November 20, 2017: Scandinavian Journal of Psychology
https://www.readbyqxmd.com/read/29149435/equine-herpesvirus-type-1-orf51-encoding-ul11-as-an-essential-gene-for-replication-in-cultured-cells
#6
Yassien Badr, Ayaka Okada, Rania Abo-Sakaya, Emad Beshir, Kenji Ohya, Hideto Fukushi
Equine herpesvirus type 1 (EHV-1) UL11 is a 74-amino-acid tegument protein encoded by ORF51 of the EHV-1 genome. EHV-1 UL11 was previously reported by other researchers using the RacL22 and RacH strains to be nonessential for viral replication in cultured cells. Here, we constructed UL11 mutant viruses including a UL11 null mutant and three C-terminal truncated mutants, for further characterization of EHV-1 UL11 using bacterial artificial chromosome (BAC) technology based on the neuropathogenic strain Ab4p...
November 17, 2017: Archives of Virology
https://www.readbyqxmd.com/read/29147023/nitrogen-related-intermediate-band-in-p-rich-ganxpyas1-x-y-alloys
#7
K Zelazna, M Gladysiewicz, M P Polak, S Almosni, A Létoublon, C Cornet, O Durand, W Walukiewicz, R Kudrawiec
The electronic band structure of phosphorus-rich GaNxPyAs1-x-y alloys (x ~ 0.025 and y ≥ 0.6) is studied experimentally using optical absorption, photomodulated transmission, contactless electroreflectance, and photoluminescence. It is shown that incorporation of a few percent of N atoms has a drastic effect on the electronic structure of the alloys. The change of the electronic band structure is very well described by the band anticrossing (BAC) model in which localized nitrogen states interact with the extended states of the conduction band of GaAsP host...
November 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29145131/removal-of-the-precursors-of-n-nitrosodiethylamine-ndea-an-emerging-disinfection-byproduct-in-drinking-water-treatment-process-and-its-toxicity-to-adult-zebrafish-danio-rerio
#8
Jian Zheng, Tao Lin, Wei Chen
N-nitrosodiethylamine (NDEA) is one of the emerging nitrogenous disinfection byproducts with probable cytotoxicity, genotoxicity, and carcinogenesis. Its potential toxicological effects have received extensive attention but remain to be poorly understood. In this study, changes in NDEA precursors in drinking water treatment process were studied using the trial of its formation potential (FP), and the toxicity induced by NDEA to adult zebrafish was investigated. NDEA FP in the raw water of Taihu Lake ranged from 46...
October 10, 2017: Chemosphere
https://www.readbyqxmd.com/read/29145119/amine-functionalized-magnetic-bamboo-based-activated-carbon-adsorptive-removal-of-ciprofloxacin-and-norfloxacin-a-batch-and-fixed-bed-column-study
#9
Xiaoming Peng, Fengping Hu, Tao Zhang, Fengxian Qiu, Hongling Dai
Amine-functionalized magnetic bamboo-based activated carbon (AFM-BAC) derived from bamboo products wastes were employed for effective adsorption of fluoroquinolone antibiotics ciprofloxacin (CIP) and norfloxacin (NOR) through batch processing. The effects of factors on the adsorption of both antibiotics were studied. The studies of various factors influencing the adsorption behavior indicated that the maximum adsorption capacities for two antibiotics adsorption were observed in weakly acidic condition and the adsorption amounts of two antibiotics increased with the increase of zwitterionic form, implying the importance of zwitterionic form, and the adsorption process is spontaneous and endothermic...
November 2, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/29143604/fine-organization-of-genomic-regions-tagged-to-the-5s-rdna-locus-of-the-bread-wheat-5b-chromosome
#10
Ekaterina M Sergeeva, Andrey B Shcherban, Irina G Adonina, Michail A Nesterov, Alexey V Beletsky, Andrey L Rakitin, Andrey V Mardanov, Nikolai V Ravin, Elena A Salina
BACKGROUND: The multigene family encoding the 5S rRNA, one of the most important structurally-functional part of the large ribosomal subunit, is an obligate component of all eukaryotic genomes. 5S rDNA has long been a favored target for cytological and phylogenetic studies due to the inherent peculiarities of its structural organization, such as the tandem arrays of repetitive units and their high interspecific divergence. The complex polyploid nature of the genome of bread wheat, Triticum aestivum, and the technically difficult task of sequencing clusters of tandem repeats mean that the detailed organization of extended genomic regions containing 5S rRNA genes remains unclear...
November 14, 2017: BMC Plant Biology
https://www.readbyqxmd.com/read/29137618/afeap-cloning-a-precise-and-efficient-method-for-large-dna-sequence-assembly
#11
Fanli Zeng, Jinping Zang, Suhua Zhang, Zhimin Hao, Jingao Dong, Yibin Lin
BACKGROUND: Recent development of DNA assembly technologies has spurred myriad advances in synthetic biology, but new tools are always required for complicated scenarios. Here, we have developed an alternative DNA assembly method named AFEAP cloning (Assembly of Fragment Ends After PCR), which allows scarless, modular, and reliable construction of biological pathways and circuits from basic genetic parts. METHODS: The AFEAP method requires two-round of PCRs followed by ligation of the sticky ends of DNA fragments...
November 14, 2017: BMC Biotechnology
https://www.readbyqxmd.com/read/29135708/high-permeability-and-intercellular-space-widening-with-brimonidine-tartrate-eye-drops-in-cultured-stratified-human-corneal-epithelial-sheets
#12
Yumi Hashimoto, Seiichi Yokoo, Tomohiko Usui, Yukiko Tsubota, Satoru Yamagami
PURPOSE: To investigate the toxicity of topical glaucoma medications using cultured stratified human corneal epithelial sheets (HCES). METHODS: HCES were exposed for 30 minutes to the following glaucoma medications: 0.1% brimonidine with sodium chlorite as the preservative, 0.005% latanoprost with 0.02% benzalkonium chloride (BAC) as the preservative, and 0.5% timolol with 0.005% BAC as the preservative. Then, cell viability and barrier function were tested by the WST-1 assay and carboxyfluorescein permeability assay, respectively...
November 13, 2017: Cornea
https://www.readbyqxmd.com/read/29128126/inhaled-cyanide-poisoning-as-a-vital-sign-in-a-room-fire-victim
#13
Christoph G Birngruber, Florian Veit, Juliane Lang, Marcel A Verhoff
The corpse of a 71-year-old woman was found on the floor of her smoke-filled room. The source of the fire was the mattress of a double bed on which newspapers had apparently been set aflame. The woman's history in conjunction with the finding situation suggested an act of suicide. No signs of soot inhalation or soot swallowing were found at autopsy. Other vital signs were absent. Severe cardiac disease was the most notable pre-existing medical condition. Although the concentration of COHb in heart blood was low (3%), the concentration of cyanide was found to be 4...
November 6, 2017: Forensic Science International
https://www.readbyqxmd.com/read/29127920/uptake-and-phytotoxic-effect-of-benzalkonium-chlorides-in-lepidium-sativum-and-lactuca-sativa
#14
Adnan Hossain Khan, Mark Libby, Daniel Winnick, John Palmer, Mark Sumarah, Madhumita B Ray, Sheila M Macfie
Cationic surfactants such as benzalkonium chlorides (BACs) are used extensively as biocides in hospitals, food processing industries, and personal care products. BACs have the potential to reach the rooting zone of crop plants and BACs might thereby enter the food chain. The two most commonly used BACs, benzyl dimethyl dodecyl ammonium chloride (BDDA) and benzyl dimethyl tetradecyl ammonium chloride (BDTA), were tested in a hydroponic system to assess the uptake by and phytotoxicity to lettuce (Lactuca sativa L...
November 8, 2017: Journal of Environmental Management
https://www.readbyqxmd.com/read/29127877/breast-arterial-calcification-on-screening-mammography-can-predict-significant-coronary-artery-disease-in-women
#15
Brendan S Kelly, Emer ScanlON, Helen Heneghan, Ciaran E Redmond, Gerard M Healy, Enda Mc Dermott, Eric J Heffernan, Ruth Prichard, Sorcha Mc Nally
INTRODUCTION: Breast Arterial Calcification (BAC) on digital mammography has been associated with an increased risk of Coronary Artery Disease (CAD). We aimed to investigate the association of BAC with findings on Coronary Computed Tomography Angiography (CCTA) within a cohort of women from the national breast screening program. METHODS: Symptomatic women (chest pain) aged between 50 and 65 who underwent a CCTA and who also had a screening mammography between 2014 and 2015 were recorded...
November 3, 2017: Clinical Imaging
https://www.readbyqxmd.com/read/29127298/sub-genome-anchored-physical-frameworks-of-the-allotetraploid-upland-cotton-gossypium-hirsutum-l-genome-and-an-approach-toward-reference-grade-assemblies-of-polyploids
#16
Christopher A Saski, Brian E Scheffler, Amanda M Hulse-Kemp, Bo Liu, Qingxin Song, Atsumi Ando, David M Stelly, Jodi A Scheffler, Jane Grimwood, Don C Jones, Daniel G Peterson, Jeremy Schmutz, Z Jeffery Chen
Like those of many agricultural crops, the cultivated cotton is an allotetraploid and has a large genome (~2.5 gigabase pairs). The two sub genomes, A and D, are highly similar but unequally sized and repeat-rich, which pose significant challenges for accurate genome reconstruction using standard approaches. Here we report the development of BAC libraries, sub genome specific physical maps, and a new-generation sequencing approach that will lead to a reference-grade genome assembly for Upland cotton. Three BAC libraries were constructed, fingerprinted, and integrated with BAC-end sequences (BES) to produce a de novo whole-genome physical map...
November 10, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29127108/egg-case-silk-gene-sequences-from-argiope-spiders-evidence-for-multiple-loci-and-a-loss-of-function-between-paralogs
#17
R Crystal Chaw, Matthew Collin, Marjorie Wimmer, Kara-Leigh Helmrick, Cheryl Y Hayashi
Spiders swath their eggs with silk to protect developing embryos and hatchlings. Egg case silks, like other fibrous spider silks, are primarily composed of proteins called spidroins (spidroin = spider-fibroin). Silks, and thus spidroins, are important throughout the lives of spiders, yet the evolution of spidroin genes has been relatively understudied. Spidroin genes are notoriously difficult to sequence because they are typically very long (10 or more kilobases of coding sequence) and highly repetitive. Here, we investigate the evolution of spider silk genes through long-read sequencing of BAC clones...
November 10, 2017: G3: Genes—Genomes—Genetics
https://www.readbyqxmd.com/read/29121399/alcohol-drinking-and-blood-alcohol-concentration-revisited
#18
Julian E Dilley, Emily Nicholson, Stephen M Fischer, Robin Zimmer, Janice C Froehlich
BACKGROUND: It is widely assumed that the amount of alcohol in the blood reflects the amount of alcohol consumed. However, several factors in addition to amount of alcohol consumed can influence blood alcohol concentration (BAC). The current study examines the effect of alcohol dose, concentration, and volume on BAC in rats with a high alcohol drinking (HAD) phenotype. METHODS: Study one examined the relationship between the amount of alcohol consumed and BAC. Alcohol-naïve, male, HAD rats (N=7) were given access to alcohol for 2 hrs/day for 9 consecutive days with food and water ad libitum...
November 9, 2017: Alcoholism, Clinical and Experimental Research
https://www.readbyqxmd.com/read/29119204/benzalkonium-chloride-and-anticancer-drugs-in-binary-mixtures-reproductive-toxicity-and-genotoxicity-in-the-freshwater-crustacean-ceriodaphnia-dubia
#19
Chiara Russo, Michael Kundi, Margherita Lavorgna, Alfredo Parrella, Marina Isidori
Benzalkonium chloride (BAC) is a cationic surfactant commonly used as a disinfectant. Its ubiquitous nature is the result of high usage and frequent discharge into the environment and evidence of interaction with numerous contaminants, such as pharmaceutical active compound residues. Anticancer drugs, among these compounds, are able to exert eco-genotoxic effects at sub ng-µg/L. The purpose of this study was to assess the reproductive toxicity and the genotoxicity of 5-fluorouracil (5-FU), cisplatin (CDDP), etoposide (ET), and imatinib mesylate (IM)-binary mixtures combined with BAC in Ceriodaphnia dubia...
November 8, 2017: Archives of Environmental Contamination and Toxicology
https://www.readbyqxmd.com/read/29118884/breast-arterial-calcifications-on-mammography-do-not-predict-myocardial-ischemia-on-myocardial-perfusion-single-photon-emission-computed-tomography
#20
Ahmed Fathala, Salma Salem, Fahad Alanazi, Deema Abunayyan, Abdelmoneim M Eldali, Abdulaziz Alsugair
Background: The aim of this study was to determine if breast arterial calcification (BAC) on mammography predicts myocardial ischemia (MI) on stress myocardial perfusion single-photon emission computed tomography (MPS). BAC is a type of medial artery calcification that can be seen incidentally on mammography, but the relationship between coronary artery calcification and MI on MPS is yet unknown. Methods: A total of 435 consecutive women underwent mammography and stress MPS within 1 year of each other...
October 2017: Cardiology Research
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