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https://www.readbyqxmd.com/read/29355484/rapid-detection-of-foodborne-pathogen-listeria-monocytogenes-by-strand-exchange-amplification
#1
Meiling Zhang, Xiudan Wang, Lingzhi Han, Shuyan Niu, Chao Shi, Cuiping Ma
A strand exchange amplification (SEA) method to detect foodborne pathogen Listeria monocytogenes was developed. SEA is a novel nucleic acid amplification method that only requires one pair of primers. The specie-specific primers were designed by targeting the 16S rRNA gene and the amplification reaction was performed as short as 60 min at 61 °C. Notably, SEA method could not only detect genomic DNA but also detect RNA by one step without requiring extra reverse transcription. The result could be visualized by naked eyes so that water bath pot would be the only equipment needed...
January 16, 2018: Analytical Biochemistry
https://www.readbyqxmd.com/read/29352085/the-expression-of-bacteriocin-production-and-self-resistance-in-lactobacillus-brevis-174a-is-mediated-by-two-regulatory-proteins
#2
Masafumi Noda, Rumi Miyauchi, Narandalai Danshiitsoodol, Yasuyuki Matoba, Takanori Kumagai, Masanori Sugiyama
We have previously shown that a lactic acid bacterium Lactobacillus (Lb.) brevis 174A isolated from a Citrus iyo fruit produces bacteriocin designated brevicin 174A, which is comprised of two antibacterial polypeptides (designated brevicin 174A-β and 174A-γ). We have also found a gene cluster, composed of eight open reading frames (ORFs), that contains genes for the biosynthesis of brevicin 174A, self-resistance to its own bacteriocin, and two transcriptional regulatory proteins.Some lactic acid bacterial strains have a system to start the production of bacteriocin at an adequate stage of the growth...
January 19, 2018: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/29349489/characterization-of-multiple-antilisterial-peptides-produced-by-sakacin-p-producing-lactobacillus-sakei-subsp-sakei-2a
#3
Kátia G Carvalho, Felipe H S Bambirra, Jacques R Nicoli, Jamil S Oliveira, Alexandre M C Santos, Marcelo P Bemquerer, Antonio Miranda, Bernadette D G M Franco
Antimicrobial compounds produced by lactic acid bacteria can be explored as natural food biopreservatives. In a previous report, the main antimicrobial compounds produced by the Brazilian meat isolate Lactobacillus sakei subsp. sakei 2a, i.e., bacteriocin sakacin P and two ribosomal peptides (P2 and P3) active against Listeria monocytogenes, were described. In this study, we report the spectrum of activity, molecular mass, structural identity and mechanism of action of additional six antilisterial peptides produced by Lb...
January 18, 2018: Archives of Microbiology
https://www.readbyqxmd.com/read/29348344/draft-genome-sequence-of-bacillus-velezensis-osy-s3-a-producer-of-potent-antimicrobial-agents-active-against-bacteria-and-fungi
#4
Michelle M Gerst, Mustafa Yesil, Ahmed E Yousef
Bacillus velezensis OSY-S3 produces anti-Listeria, anti-Escherichia coli, and antifungal compounds. Additionally, fermentate of B. velezensis OSY-S3 culture removes Staphylococcus aureus biofilms effectively. The draft genome sequence of B. velezensis OSY-S3 reported here had a genome size of ~3.90 Mb and a G+C content of 46.5%.
January 18, 2018: Genome Announcements
https://www.readbyqxmd.com/read/29348329/draft-genome-sequences-of-510-listeria-monocytogenes-strains-from-food-isolates-and-human-listeriosis-cases-from-northern-italy
#5
Sara Lomonaco, Silvia Gallina, Virginia Filipello, Maria Sanchez Leon, George John Kastanis, Marc Allard, Eric Brown, Ettore Amato, Mirella Pontello, Lucia Decastelli
Listeriosis outbreaks are frequently multistate/multicountry outbreaks, underlining the importance of molecular typing data for several diverse and well-characterized isolates. Large-scale whole-genome sequencing studies on Listeria monocytogenes isolates from non-U.S. locations have been limited. Herein, we describe the draft genome sequences of 510 L. monocytogenes isolates from northern Italy from different sources.
January 18, 2018: Genome Announcements
https://www.readbyqxmd.com/read/29345354/lack-of-functional-selectin-ligand-interactions-enhances-innate-immune-resistance-to-systemic-listeria-monocytogenes-infection
#6
Gerard Agbayani, Komal Gurnani, Ahmed Zafer, Subash Sad, Lakshmi Krishnan
Selectin-ligand interactions are important for leukocyte homing and functionality. The roles of selectin-ligand interactions in modulating immunity to intracellular infections are not completely understood. Mice lacking the expression of fucosyltransferase-IV and -VII (Fucosyltransferase-IV and -VII double knockout, FtDKO) exhibit deficient functionality of selectin-ligand interactions. We addressed the kinetics of infection and immunity to Listeria monocytogenes (LM), an intracellular pathogen, in FtDKO mice...
December 27, 2017: Journal of Leukocyte Biology
https://www.readbyqxmd.com/read/29345056/for-when-bacterial-infections-persist-toll-like-receptor-inducible-direct-antimicrobial-pathways-in-macrophages
#7
REVIEW
Claudia J Stocks, Mark A Schembri, Matthew J Sweet, Ronan Kapetanovic
Macrophages are linchpins of innate immunity, responding to invading microorganisms by initiating coordinated inflammatory and antimicrobial programs. Immediate antimicrobial responses, such as NADPH-dependent reactive oxygen species (ROS), are triggered upon phagocytic receptor engagement. Macrophages also detect and respond to microbial products through pattern recognition receptors (PRRs), such as TLRs. TLR signaling influences multiple biological processes including antigen presentation, cell survival, inflammation, and direct antimicrobial responses...
January 2018: Journal of Leukocyte Biology
https://www.readbyqxmd.com/read/29343515/discovery-of-genes-required-for-lipoteichoic-acid-glycosylation-predicts-two-distinct-mechanism-for-wall-teichoic-acid-glycosylation
#8
Jeanine Rismondo, Matthew G Percy, Angelika Gründling
The bacterial cell wall is an important and highly complex structure that is essential for bacterial growth because it protects bacteria from cell lysis and environmental insults. A typical Gram-positive bacterial cell wall is composed of peptidoglycan and the secondary cell wall polymers, wall teichoic acid (WTA) and lipoteichoic acid (LTA). In many Gram-positive bacteria, LTA is a polyglycerol-phosphate chain that is decorated with D-alanine and sugar residues. However, the function of and proteins responsible for the glycosylation of LTA are either unknown or not well-characterized...
January 17, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29339306/morphological-changes-of-bacterial-cells-upon-exposure-of-silver-silver-chloride-nanoparticles-synthesized-using-agrimonia-pilosa
#9
Maheshkumar Prakash Patil, Yong Bae Seo, Gun-Do Kim
Facile, eco-friendly synthesis of metal nanoparticles has been proposed as a cost effective method. In the present study, we propose the facile synthesis of silver-silver chloride (Ag-AgCl) nanoparticles (NPs) using the medicinally important Agrimonia pilosa plant extract without addition of capping or stabilizing agents. The Ag-AgCl NPs synthesis was observed at 40 °C after 10 min incubation; the synthesis of Ag-AgCl NPs was indicated by color change and confirmed by UV-vis spectroscopic peak at 454 nm...
January 12, 2018: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29337344/microbiological-quality-and-safety-of-fresh-fruits-and-vegetables-at-retail-levels-in-korea
#10
Charles Nkufi Tango, Shuai Wei, Imran Khan, Mohammad Shakhawat Hussain, Paul-François Ngnitcho Kounkeu, Joong-Hyun Park, Se-Hun Kim, Deog Hwan Oh
The aim of this study was to evaluate the microbiological quality and safety of fresh produce at retail level in Korea in order to periodically update information and establish available risks associated with consumption of fresh fruits and vegetables. The samples from different markets located in 3 provinces of South Korea were collected. The protocol in the Korean Food Standards Codex was applied and generic Escherichia coli, coliforms, aerobic mesophilic bacteria (AMB), and yeast and mold (YM) in 360 packaged and unpackaged fresh fruits and vegetables were analyzed...
January 16, 2018: Journal of Food Science
https://www.readbyqxmd.com/read/29337066/listeria-monocytogenes-endocarditis-case-report-review-of-the-literature-and-laboratory-evaluation-of-potential-novel-antibiotic-synergies
#11
Monika Kumaraswamy, Carter Do, George Sakoulas, Poochit Nonejuie, Guan Woei Tseng, Helen King, Joshua Fierer, Joe Pogliano, Victor Nizet
Endocarditis is a rare but serious manifestation of Listeria monocytogenes (LM). However, the optimal treatment strategy for LM endocarditis has yet to be established. Current antibiotic strategies for listeriosis include penicillin G or ampicillin (AMP) monotherapy, or AMP + gentamicin combination therapy which is often favored for endocarditis. The primary objective of our investigation was to assess the utility of AMP + ceftriaxone (CRO) and AMP + daptomycin (DAP) against LM, modeling less nephrotoxic antibiotic combinations traditionally used to manage resistant enterococcal endocarditis...
January 11, 2018: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/29329700/-a-listeria-breast-abscess-in-a-man
#12
E Marsaudon, J Berthy, S Mamoune, A Deniel, S Ksiyer, D Tiuca
INTRODUCTION: Listeriosis is a food-borne illness leading to bacteriemia or central nervous system infection especially in pregnant women or high-risk patients. It is rarely a localized infection. Breast contamination has rarely been reported in lactating women. We report a breast abscess in man. CASE REPORT: A 80 year old man, hypertensive and arrhythmic, was explored for weakness and dehydration. Type 2 diabetes and chronic lymphocytic leukemia were diagnosed...
January 9, 2018: La Revue de Médecine Interne
https://www.readbyqxmd.com/read/29329229/chemical-composition-and-evaluation-of-the-biological-properties-of-the-essential-oil-of-the-dietary-phytochemical-lippia-citriodora
#13
Eleni Fitsiou, Gregoria Mitropoulou, Katerina Spyridopoulou, Manolis Vamvakias, Haido Bardouki, Alex Galanis, Katerina Chlichlia, Yiannis Kourkoutas, Mihalis Ι Panayiotidis, Aglaia Pappa
The aim of the study was to characterize the chemical composition and biological properties of the essential oil from the plant Lippia citriodora grown in Greece. The essential oil volatiles were analyzed by gas chromatography-mass spectrometry GC-MS indicating citral as the major component. Τhe antimicrobial properties were assayed using the disk diffusion method and the minimum inhibitory and non-inhibitory concentration values were determined. Listeria monocytogenes, Staphylococcus epidermidis, Staphylococcus aureus, Saccharomyces cerevisiae, and Aspergillus niger were sensitive to Lippia citriodora oil, but not Escherichia coli, Salmonella Enteritidis, Salmonella typhimurium, and Pseudomonas fragi...
January 12, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29326391/zoonotic-bacteria-and-parasites-found-in-raw-meat-based-diets-for-cats-and-dogs
#14
Freek P J van Bree, Gertie C A M Bokken, Robin Mineur, Frits Franssen, Marieke Opsteegh, Joke W B van der Giessen, Len J A Lipman, Paul A M Overgaauw
Feeding raw meat-based diets (RMBDs) to companion animals has become increasingly popular. Since these diets may be contaminated with bacteria and parasites, they may pose a risk to both animal and human health. The purpose of this study was to test for the presence of zoonotic bacterial and parasitic pathogens in Dutch commercial RMBDs. We analysed 35 commercial frozen RMBDs from eight different brands. Escherichia coli serotype O157:H7 was isolated from eight products (23 per cent) and extended-spectrum beta-lactamases-producing E coli was found in 28 products (80 per cent)...
January 13, 2018: Veterinary Record
https://www.readbyqxmd.com/read/29326266/the-epigenetic-control-of-stemness-in-cd8-t-cell-fate-commitment
#15
Luigia Pace, Christel Goudot, Elina Zueva, Paul Gueguen, Nina Burgdorf, Joshua J Waterfall, Jean-Pierre Quivy, Geneviève Almouzni, Sebastian Amigorena
After priming, naïve CD8+ T lymphocytes establish specific heritable transcription programs that define progression to long-lasting memory cells or to short-lived effector cells. Although lineage specification is critical for protection, it remains unclear how chromatin dynamics contributes to the control of gene expression programs. We explored the role of gene silencing by the histone methyltransferase Suv39h1. In murine CD8+ T cells activated after Listeria monocytogenes infection, Suv39h1-dependent trimethylation of histone H3 lysine 9 controls the expression of a set of stem cell-related memory genes...
January 12, 2018: Science
https://www.readbyqxmd.com/read/29324702/listeria-monocytogenes-the-impact-of-cell-death-on-infection-and-immunity
#16
REVIEW
Courtney E McDougal, John-Demian Sauer
Listeria monocytogenes has evolved exquisite mechanisms for invading host cells and spreading from cell-to-cell to ensure maintenance of its intracellular lifecycle. As such, it is not surprising that loss of the intracellular replication niche through induction of host cell death has significant implications on the development of disease and the subsequent immune response. Although L. monocytogenes can activate multiple pathways of host cell death, including necrosis, apoptosis, and pyroptosis, like most intracellular pathogens L...
January 11, 2018: Pathogens
https://www.readbyqxmd.com/read/29324679/antibacterial-evaluation-and-virtual-screening-of-new-thiazolyl-triazole-schiff-bases-as-potential-dna-gyrase-inhibitors
#17
Cristina Nastasă, Dan C Vodnar, Ioana Ionuţ, Anca Stana, Daniela Benedec, Radu Tamaian, Ovidiu Oniga, Brînduşa Tiperciuc
The global spread of bacterial resistance to drugs used in therapy requires new potent and safe antimicrobial agents. DNA gyrases represent important targets in drug discovery. Schiff bases, thiazole, and triazole derivatives are considered key scaffolds in medicinal chemistry. Fifteen thiazolyl-triazole Schiff bases were evaluated for their antibacterial activity, measuring the growth inhibition zone diameter, the minimum inhibitory concentration (MIC), and the minimum bactericidal concentration (MBC), against Gram-positive (Staphylococcus aureus, Listeria monocytogenes) and Gram-negative (Escherichia coli, Salmonella typhimurium, Pseudomonas aeruginosa) bacteria...
January 11, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29322608/comparison-of-listeriolysin-o-and-phospholipases-plca-and-plcb-activities-and-initial-intracellular-growth-capability-among-food-and-clinical-strains-of-listeria-monocytogenes
#18
M Kanki, H Naruse, K Kawatsu
AIMS: We investigated whether Listeria monocytogenes strains differ in their ability to escape from the primary phagosome after internalization into human intestinal epithelial cells. METHODS AND RESULTS: Food and clinical strains were used to study specific alleles; the activities of listeriolysin O (LLO) and phospholipases PlcA and PlcB, which promote rupture of the phagocytic vacuole; and initial intracellular bacterial growth in Caco-2 cells. Results showed no difference in LLO activities between food and clinical strains or among serotypes...
January 11, 2018: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/29320565/the-absence-of-n-acetylglucosamine-in-wall-teichoic-acids-of-listeria-monocytogenes-modifies-biofilm-architecture-and-tolerance-to-rinsing-and-cleaning-procedures
#19
Thomas Brauge, Christine Faille, Irina Sadovskaya, Alain Charbit, Thierry Benezech, Yang Shen, Martin J Loessner, Jean Romain Bautista, Graziella Midelet-Bourdin
The wall teichoic acid (WTA) is the major carbohydrate found within the extracellular matrix of the Listeria monocytogenes biofilm. We first addressed the frequency of spontaneous mutations in two genes (lmo2549 and lmo2550) responsible for the GlcNAcylation in 93 serotype 1/2a strains that were mainly isolated from seafood industries. We studied the impact of mutations in lmo2549 or lmo2550 genes on biofilm formation by using one mutant carrying a natural mutation inactivating the lmo2550 gene (DSS 1130 BFA2 strain) and two EGD-e mutants that lack respective genes by in-frame deletion of lmo2549 or lmo2550 using splicing-by-overlap-extension PCR, followed by allelic exchange mutagenesis...
2018: PloS One
https://www.readbyqxmd.com/read/29320233/antibacterial-activities-of-hibiscus-sabdariffa-extracts-and-chemical-sanitizers-directly-on-green-leaves-contaminated-with-foodborne-pathogens
#20
Carlos A Gómez-Aldapa, Esmeralda Rangel-Vargas, Ma Refugio Torres-Vitela, Angélica Villarruel-López, Otilio A Acevedo-Sandoval, Alberto J Gordillo-Martínez, Angélica Godínez-Oviedo, Javier Castro-Rosas
Leafy greens have been associated with foodborne disease outbreaks in different countries. To decrease microbial contamination of leafy greens, chemical agents are commonly used; however, a number of studies have shown these agents to have limited antimicrobial effect against pathogenic bacteria on vegetables. The objective of this study was to compare the antibacterial effect of Hibiscus sabdariffa calyx extracts (water, methanol, acetone, and ethyl acetate), sodium hypochlorite, acetic acid, and colloidal silver against foodborne bacteria on leafy greens...
January 10, 2018: Journal of Food Protection
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