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https://www.readbyqxmd.com/read/28448066/structural-basis-of-crispr-spycas9-inhibition-by-an-anti-crispr-protein
#1
De Dong, Minghui Guo, Sihan Wang, Yuwei Zhu, Shuo Wang, Zhi Xiong, Jianzheng Yang, Zengliang Xu, Zhiwei Huang
The CRISPR-Cas9 systems are bacterial encoded adaptive immune systems to defend against phages infection, through RNA-guided endonuclease activity of Cas9 to degrade double-stranded DNA bearing a protospacer adjacent motif (PAM) and complementary sequences to the guide RNA1,2,3,4,5. Recently, two anti-CRISPR proteins AcrIIA2 and AcrIIA4 from Listeria monocytogenes (Lmo) prophages have been identified, both of which potently inhibit Streptococcus pyogenes Cas9 (SpyCas9) and LmoCas9 activity in bacteria and human cells6...
April 27, 2017: Nature
https://www.readbyqxmd.com/read/28445701/prevalence-antimicrobial-susceptibility-and-multiplex-pcr-serotyping-of-listeria-monocytogenes-isolated-from-humans-foods-and-livestock-in-iran
#2
Lida Lotfollahi, Ardalan Chaharbalesh, Mohammad Ahangarzadeh Rezaee, Alka Hasani
Listeria monocytogenes is a foodborne pathogen causing listeriosis, which potentially affects all individuals, especially pregnant women and immunocompromised persons. The present study investigated the prevalence, antimicrobial susceptibility and serotypes distribution of the isolated L. monocytogenes from Iran. Twenty two (4.97%) of 442 human, food and livestock samples were found to be positive for L. monocytogenes. L. monocytogenes was identified in 8.8% of 125 human samples, 2.99% of 267 food and 6% of 50 livestock samples...
April 23, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28441322/erratum-kim-k-p-singh-a-k-bai-x-leprun-l-bhunia-a-k-novel-pcr-assays-complement-laser-biosensor-based-method-and-facilitate-listeria-species-detection-from-food-sensors-2015-15-22672-22691
#3
Kwang-Pyo Kim, Atul K Singh, Xingjian Bai, Lena Leprun, Arun K Bhunia
The authors wish to correct the oligonucleotide sequence of primer E-LAP-F1 and LIS-R1 in Table 1in their paper published in Sensors [1], doi:10.3390/s150922672, http://www.mdpi.com/1424-8220/15/9/22672. The following table should be used.[...].
April 25, 2017: Sensors
https://www.readbyqxmd.com/read/28437165/survey-for-listeria-monocytogenes-in-and-on-ready-to-eat-foods-from-retail-establishments-in-the-united-states-2010-through-2013-assessing-potential-changes-of-pathogen-prevalence-and-levels-in-a-decade
#4
John B Luchansky, Yuhuan Chen, Anna C S Porto-Fett, Régis Pouillot, Bradley A Shoyer, Rachel Johnson-DeRycke, Denise R Eblen, Karin Hoelzer, William K Shaw, Jane M van Doren, Michelle Catlin, Jeehyun Lee, Rohan Tikekar, Daniel Gallagher, James A Lindsay, Sherri Dennis
A multiyear interagency Listeria monocytogenes Market Basket Survey was undertaken for selected refrigerated ready-to-eat foods purchased at retail in four FoodNet sites in the United States. Food samples from 16 food categories in six broad groups (seafood, produce, dairy, meat, eggs, and combination foods) were collected weekly at large national chain supermarkets and independent grocery stores in California, Maryland, Connecticut, and Georgia for 100 weeks between December 2010 and March 2013. Of the 27,389 total samples, 116 samples tested positive by the BAX PCR system for L...
April 24, 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/28434737/short-communication-microbiological-quality-of-raw-cow-milk-and-its-association-with-herd-management-practices-in-northern-china
#5
X Y Lan, S G Zhao, N Zheng, S L Li, Y D Zhang, H M Liu, J McKillip, J Q Wang
Contamination of raw milk with bacterial pathogens is potentially hazardous to human health. The aim of this study was to evaluate the total bacteria count (TBC) and presence of pathogens in raw milk in Northern China along with the associated herd management practices. A total of 160 raw milk samples were collected from 80 dairy herds in Northern China. All raw milk samples were analyzed for TBC and pathogens by culturing. The results showed that the number of raw milk samples with TBC <2 × 10(6) cfu/mL and <1 × 10(5) cfu/mL was 146 (91...
April 20, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28433889/quantitative-proteomic-profiling-for-clarification-of-the-crucial-roles-of-lysosomes-in-microbial-infections
#6
Benhong Xu, Yanpan Gao, Shaohua Zhan, Wei Ge
Lysosomes play vital roles in both innate and adaptive immunity. It is widely accepted that lysosomes do not function exclusively as a digestive organelle. It is also involved in the process of immune cells against pathogens. However, the changes in the lysosomal proteome caused by infection with various microbes are still largely unknown, and our understanding of the proteome of the purified lysosome is another obstacle that needs to be resolved. Here, we performed a proteomic study on lysosomes enriched from THP1 cells after infection with Listeria monocytogenes (L...
April 20, 2017: Molecular Immunology
https://www.readbyqxmd.com/read/28428781/analysis-of-the-listeria-monocytogenes-population-structure-among-isolates-from-1931-to-2015-in-australia
#7
Amy V Jennison, Jesse J Masson, Ning-Xia Fang, Rikki M Graham, Mark I Bradbury, Narelle Fegan, Kari S Gobius, Trudy M Graham, Christine J Guglielmino, Janelle L Brown, Edward M Fox
Listeriosis remains among the most important bacterial illnesses, with a high associated mortality rate. Efforts to control listeriosis require detailed knowledge of the epidemiology of the disease itself, and its etiological bacterium, Listeria monocytogenes. In this study we provide an in-depth analysis of the epidemiology of 224 L. monocytogenes isolates from Australian clinical and non-clinical sources. Non-human sources included meat, dairy, seafood, fruit, and vegetables, along with animal and environmental isolates...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28428307/draft-genome-sequences-of-three-listeria-monocytogenes-isolates-from-foods-in-china
#8
Xudong Su, Guojie Cao, Dai Kuang, Jianmin Zhang, Yi Chen, Marc Allard, Eric Brown, Xianming Shi, Jianghong Meng, Xuebin Xu
Listeria monocytogenes is a foodborne pathogen of global concern because of the high mortality rate among patients. The draft genome sequences of three L. monocytogenes strains isolated from foods are reported here. The availability of these genomes should provide useful information on the genomic diversity of L. monocytogenes isolated from foods in China.
April 20, 2017: Genome Announcements
https://www.readbyqxmd.com/read/28427354/unique-case-report-of-a-chromomycosis-and-listeria-in-soft-tissue-and-cerebellar-abscesses-after-kidney-transplantation
#9
J Tourret, N Benabdellah, S Drouin, F Charlotte, J Rottembourg, N Arzouk, A Fekkar, B Barrou
BACKGROUND: Chromomycosis is a rare mycotic infection encountered in tropical and subtropical regions. The disease presents as a slowly-evolving nodule that can become infected with bacteria. Here, we describe a unique association of abscesses caused by a chromomycosis and Listeria monocytogenes in a kidney transplant recipient, and didactically expose how the appropriate diagnosis was reached. CASE PRESENTATION: A 49-year old male originating from the Caribbean presented a scalp lesion which was surgically removed in his hometown where it was misdiagnosed as a sporotrichosis on histology, 3 years after he received a kidney transplant...
April 20, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28425569/a-comparative-study-of-natural-antimicrobial-delivery-systems-for-microbial-safety-and-quality-of-fresh-cut-lettuce
#10
Laura E Hill, Daniela A Oliveira, Katherine Hills, Cassie Giacobassi, Jecori Johnson, Harvey Summerlin, T Matthew Taylor, Carmen L Gomes
Nanoencapsulation can provide a means to effectively deliver antimicrobial compounds and enhance the safety of fresh produce. However, to date there are no studies which directly compares how different nanoencapsulation systems affect fresh produce safety and quality. This study compared the effects on quality and safety of fresh-cut lettuce treated with free and nanoencapsulated natural antimicrobial, cinnamon bark extract (CBE). A challenge study compared antimicrobial efficacy of 3 different nanoencapsulated CBE systems...
April 20, 2017: Journal of Food Science
https://www.readbyqxmd.com/read/28420823/listeria-monocytogenes-serotype-4b-strains-replicate-in-monocytes-macrophages-more-than-the-other-serotypes
#11
Rie Hasebe, Ryo Nakao, Aiko Ohnuma, Takeshi Yamasaki, Hirofumi Sawa, Shinji Takai, Motohiro Horiuchi
We analyzed the pathogenicity of various serotypes of Listeria monocytogenes using a Balb/c mouse intravenous injection model. The survival rates of mice inoculated with strains NS1/2b (serotype 1/2b), NS3b (serotype 3b) and NS 4b (serotype 4b) were 60%, 63.6% and 63.6%, respectively. Although the survival rates were similar, the bacterial growth in the liver of NS3b-infected mice was 144.5-fold higher than that in the liver of NS4b-infected mice. Histopathological analyses suggest that the NS4b strain replicated more in monocytes/macrophages, whereas the NS3b strain replicated more in hepatocytes...
April 17, 2017: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/28419853/listeria-monocytogenes-meningitis-in-the-netherlands-1985-2014-a-nationwide-surveillance-study
#12
Merel M Koopmans, Merijn W Bijlsma, Matthijs C Brouwer, Diederik van de Beek, Arie van der Ende
OBJECTIVES: Listeria monocytogenes can cause sepsis and meningitis. We report national surveillance data on L. monocytogenes meningitis in the Netherlands, describing incidence changes, genetic epidemiology and fatality rate. METHODS: We analyzed data from the Netherlands Reference Laboratory of Bacterial Meningitis for cases of L.monocytogenes meningitis. Strains were assessed by serotyping and bacterial population structure by multi-locus sequence typing. RESULTS: A total of 375 cases of Listeria meningitis were identified between 1985 and 2014...
April 15, 2017: Journal of Infection
https://www.readbyqxmd.com/read/28419297/host-pore-forming-protein-complex-neutralizes-the-acidification-of-endocytic-organelles-to-counteract-intracellular-pathogens
#13
Sheng-An Li, Long Liu, Xiao-Long Guo, Yu-Yan Zhang, Yang Xiang, Qi-Quan Wang, Wen-Hui Lee, Yun Zhang
Many intracellular pathogens invade cells via endocytic organelles and have adapted to the drop in pH along the endocytic pathway. However, the strategy by which the host cell counteracts this pathogen adaptation remains unclear. βγ-CAT is an aerolysin-like pore-forming protein and trefoil factor complex in the frog Bombina maxima. We report here that βγ-CAT, as a host-secreted factor with an intrinsic channel-forming property, is the first example of a molecule that actively neutralizes the acidification of endocytic organelles to counteract Listeria monocytogenes infection...
April 17, 2017: Journal of Infectious Diseases
https://www.readbyqxmd.com/read/28418717/diversity-of-endophytic-bacteria-in-a-fern-species-dryopteris-uniformis-makino-makino-and-evaluation-of-their-antibacterial-potential-against-five-foodborne-pathogenic-bacteria
#14
Gitishree Das, Seonjoo Park, Kwang-Hyun Baek
The fern plant Dryopteris uniformis has traditionally been used in herbal medicine and possesses many biological activities. This study was conducted to explore the endophytic bacterial diversity associated with D. uniformis and evaluate their antibacterial potential against foodborne pathogenic bacteria (FPB). Among 51 isolated endophytic bacteria (EB), 26 EB were selected based on their morphological characteristics and identified by 16S rRNA gene analysis. The distribution of EB was diverse in the leaf and the stem/root tissues...
April 18, 2017: Foodborne Pathogens and Disease
https://www.readbyqxmd.com/read/28416883/application-of-electrolysed-oxidising-water-as-a-sanitiser-to-extend-the-shelf-life-of-seafood-products-a-review
#15
REVIEW
Fera R Dewi, Roger Stanley, Shane M Powell, Christopher M Burke
Electrolysed oxidising water (E.O. water) is produced by electrolysis of sodium chloride to yield primarily chlorine based oxidising products. At neutral pH this results in hypochlorous acid in the un-protonated form which has the greatest oxidising potential and ability to penetrate microbial cell walls to disrupt the cell membranes. E.O. water has been shown to be an effective method to reduce microbial contamination on food processing surfaces. The efficacy of E.O. water against pathogenic bacteria such as Listeria monocytogenes, Escherichia coli and Vibrio parahaemolyticus has also been extensively confirmed in growth studies of bacteria in culture where the sanitising agent can have direct contact with the bacteria...
April 2017: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/28414258/postchill-antimicrobial-treatments-to-control-salmonella-listeria-and-campylobacter-contamination-on-chicken-skin-used-in-ground-chicken
#16
Suejee Park, Mark A Harrison, Mark E Berrang
Ground poultry products are frequently contaminated with foodborne pathogens. With the potential for increased regulatory scrutiny, it is important to use sufficient intervention strategies to control pathogen levels effectively. A large proportion of the bacteria introduced to ground chicken are likely to come from broiler skin, which is added to achieve target fat content and maintain product texture and taste. In this research, antimicrobials, including 50 ppm of chlorine and 1,200 ppm of peracetic acid (PAA), were applied in a postchill system to reduce the number of Salmonella Typhimurium, Listeria monocytogenes , and Campylobacter coli inoculated on chicken skin used to formulate ground chicken...
April 17, 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/28411150/comparative-diagnostic-efficacy-of-recombinant-llo-and-pi-plc-based-elisas-for-detection-of-listeriosis-in-animals
#17
Rahul D Suryawanshi, Satya Veer Singh Malik, Bhushan Jayarao, Sandeep P Chaudhari, Emily Savage, Jess Vergis, Nitin V Kurkure, Sukhadeo B Barbuddhe, Deepak B Rawool
The present study for the first time evaluates the serodiagnostic efficacy of two recombinant antigens namely, listeriolysin O (rLLO) and phosphatidyl-inositol phospholipase C (rPI-PLC). Indirect ELISA with the above recombinant antigens was used on samples collected from bovines (n=106), goats (n=138) and pigs (n=92) having either a history of abortion, emaciation and/or apparently healthy animals. Isolation of Listeria was attempted from the blood samples using USDA-FSIS method. On screening of test sera by rLLO-based ELISA, antibodies against anti-listeriolysin O (ALLO) were observed in goats (22...
April 12, 2017: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/28408492/cd81-as-a-tumor-target
#18
REVIEW
Felipe Vences-Catalán, Caroline Duault, Chiung-Chi Kuo, Ranjani Rajapaksa, Ronald Levy, Shoshana Levy
CD81 participates in a variety of important cellular processes such as membrane organization, protein trafficking, cellular fusion and cell-cell interactions. In the immune system, CD81 regulates immune synapse, receptor clustering and signaling; it also mediates adaptive and innate immune suppression. CD81 is a gateway in hepatocytes for pathogens such as hepatitis C virus and Plasmodium; it also confers susceptibility to Listeria infection. These diverse biological roles are due to the tendency of CD81 to associate with other tetraspanins and with cell-specific partner proteins, which provide the cells with a signaling platform...
April 15, 2017: Biochemical Society Transactions
https://www.readbyqxmd.com/read/28407186/antimicrobial-activity-and-chemical-composition-of-brunfelsia-uniflora-flower-oleoresin-extracted-by-supercritical-carbon-dioxide
#19
L C T Thiesen, E Y Y Sugauara, V Tešević, J Glamočlija, M Soković, J E Gonçalves, Z C Gazim, G A Linde, N B Colauto
Brunfelsia genus is traditionally utilized in popular medicine due to its antibacterial and antifungal properties to name but a few. However, studies on the antimicrobial activity of Brunfelsia uniflora flower oleoresin have not been found yet. This study aimed to evaluate the chemical composition and antimicrobial activity of B. uniflora flower oleoresin obtained by supercritical carbon dioxide. Oleoresin from the plant dried flowers was obtained by carbon dioxide, and the chemical composition was analyzed by gas chromatographic-mass spectrometry...
April 13, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28401466/antibacterial-effects-of-lactobacillus-isolates-of-curd-and-human-milk-origin-against-food-borne-and-human-pathogens
#20
Chetan Sharma, Brij Pal Singh, Nishchal Thakur, Sachin Gulati, Sanjolly Gupta, Santosh Kumar Mishra, Harsh Panwar
This study was undertaken to assess the antibacterial efficacy of lactobacilli isolated from curd and human milk samples. Identities of thirty-one different lactobacilli (20 from curd and 11 from human milk) were confirmed by genus-specific PCR and 16S rRNA-based sequencing. These strains belonged to five species, Lactobacillus casei, L. delbrueckii, L. fermentum, L. plantarum, and L. pentosus. Antibacterial activities of cell-free supernatants (CFSs) of all the Lactobacillus isolates were estimated through standard agar-well diffusion assay, against commonly occurring food-borne and clinically important human pathogens...
May 2017: 3 Biotech
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