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Dino Miraglia, David Ranucci, Massimo Trabalza-Marinucci, Gabriele Acuti, Claudio Forte, Michela Codini, Rossana Roila, Raffaella Branciari
This study aimed at evaluating the effect of Oregano vulgaris extract supplementation of heavy pig diet on the microbiological, chemical-physical and sensory characteristics of Fabriano salami. Fabriano salamis were produced processing meat derived from pigs belonging to two dietary groups of 16 pigs each: CTRL group fed a commercial pelleted feed, and OR group fed the CTRL diet integrated with 0.2% of oregano extract. Ten salamis per group were collected at 0, 7, 20 and 45 days of ripening and analysed for total viable count, enumeration of Enterobacteriaceae , Enterococcus spp, coagulase negative and positive staphylococci, Lactobacillus spp...
October 20, 2017: Italian Journal of Food Safety
Erica Tirloni, Cristian Bernardi, Sandro Drago, Giuseppe Stampone, Francesco Pomilio, Patrizia Cattaneo, Simone Stella
Objective of the present study was to test the performances of a loop-mediated isothermal amplification (LAMP)-based method for the detection of Listeria monocytogenes , with particular focus on the dairy products. The specificity of the method was evaluated on 42 different Listeria spp. strains from collections, food and environmental samples. 100% (32 of 32) of the L. monocytogenes strains were correctly recognised, and none of other 10 Listeria spp. strains was misidentified. The sensitivity was evaluated on four L...
October 20, 2017: Italian Journal of Food Safety
Folachodé U G Akogou, Heidy M W den Besten, A P Polycarpe Kayodé, Vincenzo Fogliano, Anita R Linnemann
Sorghum (Sorghum bicolor) extract is traditionally used as red biocolorant in West Africa to colour foods, among which wagashi, a soft cheese. This biocolorant is a source of the phytoalexin apigeninidin and phenolic acids, and users claim that it has preservative effects next to its colouring properties. If such a claim can be scientifically substantiated, it adds a valuable functional property to this natural red colorant, thereby increasing its potential applications in the food industry. Hence, the present study evaluated the antimicrobial properties of dye sorghum extracts using challenge tests in broth and wagashi as a model of a popular food application...
2018: PloS One
Christoph Jans, Eleonora Sarno, Lucie Collineau, Leo Meile, Katharina D C Stärk, Roger Stephan
Background: Antimicrobial resistance (AMR) in bacteria is an increasing health concern. The spread of AMR bacteria (AMRB) between animals and humans via the food chain and the exchange of AMR genes requires holistic approaches for risk mitigation. The AMRB exposure of humans via food is currently only poorly understood leaving an important gap for intervention design. Method: This study aimed to assess AMRB prevalence in retail food and subsequent exposure of Swiss consumers in a systematic literature review of data published between 1996 and 2016 covering the Swiss agriculture sector and relevant imported food...
2018: Frontiers in Microbiology
Carlos Rumbo, Juan Antonio Tamayo-Ramos, M Federica Caso, Antonio Rinaldi, Lorena Romero-Santacreu, Roberto Quesada, Santiago Cuesta
Electrospun biodegradable polymers have emerged as promising materials for their applications in several fields, including biomedicine and food industry. For this reason, the susceptibility of these materials to be colonized by different pathogens is a critical issue for public health, and their study can provide future knowledge to develop new strategies against bacterial infections. In this work, the ability of three pathogenic bacterial species ( Pseudomonas aeruginosa, Acinetobacter baumannii and Listeria monocytogenes) to adhere and form biofilm in electrospun polycaprolactone (PCL) microfibrous meshes was investigated...
March 20, 2018: ACS Applied Materials & Interfaces
Natalia Crespo Tapia, Heidy M W den Besten, Tjakko Abee
Listeria monocytogenes is a food-borne pathogen that can grow as a biofilm on surfaces. Biofilm formation in food-processing environments is a big concern for food safety, as it can cause product contamination through the food-processing line. Although motile aerobic bacteria have been described to form biofilms at the air-liquid interface of cell cultures, to our knowledge, this type of biofilm has not been described in L. monocytogenes before. In this study we report L. monocytogenes biofilm formation at the air-liquid interface of aerobically grown cultures, and that this phenotype is specifically induced when the media is supplemented with glycerol as a carbon and energy source...
March 15, 2018: International Journal of Food Microbiology
Amber Hilliard, Dara Leong, Amy O'Callaghan, Eamonn P Culligan, Ciara A Morgan, Niall DeLappe, Colin Hill, Kieran Jordan, Martin Cormican, Cormac G M Gahan
Listeria monocytogenes is a major human foodborne pathogen that is prevalent in the natural environment and has a high case fatality rate. Whole genome sequencing (WGS) analysis has emerged as a valuable methodology for the classification of L. monocytogenes isolates and the identification of virulence islands that may influence infectivity. In this study, WGS was used to provide an insight into 25 L. monocytogenes isolates from cases of clinical infection in Ireland between 2013 and 2015. Clinical strains were either lineage I (14 isolates) or lineage II (11 isolates), with 12 clonal complexes (CC) represented, of which CC1 (6) and CC101 (4) were the most common...
March 20, 2018: Genes
Akiko Yamazaki, Mioko Honda, Naoki Kobayashi, Naoto Ishizaki, Hiroshi Asakura, Yoshiko Sugita-Konishi
The expansion of the wild deer population is a major problem for the Japanese farm and forestry industries because their damage to farm products and vegetation results in huge economic loss. To promote game meat consumption, hygiene inspections should be performed to detect main bacterial pathogens before products are shipped. In this study, we aimed to evaluate the ability of commercial test kits to genetically detect EHEC, Salmonella and Listeria monocytogenes in venison. Our results demonstrated that the kits for three pathogens could be useful for venison as well as other domestic meat products...
March 19, 2018: Journal of Veterinary Medical Science
Siyue Gao, Brooke Pearson, Lili He
The existence of pathogenic bacteria in drinking water has been a threat to the safety of human well-being. Traditional methods to detect bacteria are standard plate counts or rapid methods such as ELISA and PCR. However, those methods can be time-consuming or require pre-enrichment when detecting low concentrations of bacteria. Filter membrane has been used for bacteria collection and culturing when monitoring environmental water samples. In this study, we applied surface enhanced Raman spectroscopy (SERS) to rapidly screen bacteria cells on a filter membrane based on the unique signal from an indicator molecule, 4-mercaptophenylboronic acid (4-mpba), that can specifically bind to the surface of bacteria through diol group in its structure and give off consistent SERS signal...
March 15, 2018: Journal of Microbiological Methods
Ashokkumar Sekar, Myoungjin Kim, Hyeong Chul Jeong, Keun Kim
Lactobacillus pentosus K1-23 was selected from among 25 lactic acid bacterial strains due to its high inhibitory activity against several pathogenic bacteria, including Escherichia coli, Salmonella typhimurium, S. gallinarum, Staphylococcus aureus, Pseudomonas aeruginosa, Clostridium perfringens , and Listeria monocytogenes . Additionally, among 13 strains of Aureobasidium spp., A. pullulans NRRL 58012 was shown to produce the highest amount of β-glucan (15.45 ± 0.07%) and was selected. Next, the optimal conditions for a solid-phase mixed culture with these two different microorganisms, one bacterium and one yeast, were determined...
March 16, 2018: Journal of Microbiology and Biotechnology
Qian Zhang, Satoshi Ishii
Quantitative polymerase chain reaction (qPCR) is now commonly used to detect fecal indicator bacteria (FIB) as well as pathogens in water samples. However, DNA loss during sample processing can cause underestimation of target genes. In this study, we created a sample process control strain (SPC) by genetically engineering a non-pathogenic, Gram-negative bacterium Pseudogulbenkiania sp. strain NH8B. The SPC strain, named NH8B-1D2, has a kanamycin-resistance gene inserted to one of the 23S rRNA genes. To specifically quantify the SPC strain, a new TaqMan qPCR assay was developed...
March 10, 2018: Water Research
Marjolaine Morgand, Alexandre Leclercq, Mylène M Maury, Hélène Bracq-Dieye, Pierre Thouvenot, Guillaume Vales, Marc Lecuit, Caroline Charlier
OBJECTIVES: Listeria monocytogenes (Lm) is a foodborne human pathogen responsible for severe infection, including septicemia, neurolisteriosis, maternal-fetal and focal infections. Little is known about Lm-associated respiratory tract or lung infections. METHODS: Retrospective study of culture-proven cases of Lm pleural infections and pneumonia reported to the French National Reference Center for Listeria from January 1993 to August 2016. RESULTS: Thirty-eight consecutive patients with pleural infection (n=32), pneumonia (n=5), or both (n=1) were studied...
March 13, 2018: Clinical Microbiology and Infection
Jiyoun Min, Dongchan Yang Sung, Mirang Kim, Keeok Haam, Anji Yoo, Jae-Hoon Choi, Barbara U Schraml, Yong Sung Kim, Dongsup Kim, Suk-Jo Kang
The spatiotemporal regulation of immune cells in lymph nodes (LNs) is crucial for mounting protective T-cell responses, which are orchestrated by dendritic cells (DCs). However, it is unclear how the DC subsets are altered by the inflammatory milieu of LNs. Here, we show that the inflamed LNs of Listeria-infected mice are characterized by the clustering of neutrophils and monocytes and IFN-γ production. Significantly, the early inflammatory responses are coupled with the differentiation of not one, but two types of CD64+ CD11c+ MHCII+ inflammatory DCs...
March 16, 2018: Experimental & Molecular Medicine
Danielle R Balay, Michael G Gänzle, Lynn M McMullen
The aim of this study was to determine if different carbohydrates influence the growth of Listeria monocytogenes in the presence of carnocyclin A or leucocin A. Carnobacterium maltaromaticum ATCC PTA-5313 and Leuconostoc gelidum UAL187 were used to produce carnocyclin A and leucocin A, respectively. Growth curves were modeled for five strains of L. monocytogenes grown in basal medium supplemented with glucose, sucrose, fructose, mannose, or cellobiose, in the presence of carnocyclin A or leucocin A. The growth of L...
2018: Frontiers in Microbiology
Alexander Gluschko, Marc Herb, Katja Wiegmann, Oleg Krut, Wolfram F Neiss, Olaf Utermöhlen, Martin Krönke, Michael Schramm
The intracellular pathogen Listeria monocytogenes (L.m.) is targeted by the autophagic machinery, but the molecular mechanisms involved and consequences for anti-listerial immunity remain enigmatic. Here, we demonstrate that L.m. infection of macrophages in vivo exclusively evokes LC3-associated phagocytosis (LAP), but not canonical autophagy, and that targeting of L.m. by LAP is required for anti-listerial immunity. The pathway leading to LAP induction in response to L.m. infection emanates from the β2 integrin Mac-1 (CR3, integrin αM β2 ), a receptor recognizing diverse microbial ligands...
March 14, 2018: Cell Host & Microbe
Katherine D Hills, Daniela A Oliveira, Nicholas D Cavallaro, Carmen L Gomes, Eric S McLamore
We demonstrate a sensing mechanism for rapid detection of Listeria monocytogenes in food samples using the actuation of chitosan-aptamer nanobrush borders. The bio-inspired soft material and sensing strategy mimic natural symbiotic systems, where low levels of bacteria are selectively captured from complex matrices. To engineer this biomimetic system, we first develop reduced graphene oxide/nanoplatinum (rGO-nPt) electrodes, and characterize the fundamental electrochemical behavior in the presence and absence of chitosan nanobrushes during actuation (pH-stimulated osmotic swelling)...
March 15, 2018: Analyst
Irene Ortega Blázquez, María J Grande Burgos, Rubén Pérez-Pulido, Antonio Gálvez, Rosario Lucas
The aim of this study was to determine the impact of activated plastic films with thymol and enterocin AS-48 and high-hydrostatic pressure (HP) treatment on the bacterial load and bacterial diversity of vacuum-packaged sea bream fillets under refrigerated storage for 10 days. The activated film and the HP treatment reduced aerobic mesophiles viable counts by 1.46 and 2.36 log cycles, respectively, while the combined treatment achieved a reduction of 4.13 log cycles. HP and combined treatments resulted in longer delays in bacterial growth...
2018: Frontiers in Microbiology
Eleni C Pappa, Thomas G Bontinis, Maria Tasioula-Margari, John Samelis
The microbiological quality of and changes in the main physicochemical parameters, together with the evolution of proteolysis, lipolysis and volatile profiles of soft Xinotyri, a traditional Greek acid-curd cheese (pH≈4.4, moisture 65%, salt 1%) made from raw (RMC) or pasteurized (PMC) goat's milk without starters, were evaluated during aerobic storage at 4o C for 60 days. No statistically significant differences between the total nitrogen (TN) and nitrogen fraction (% of TN) contents, the degradation of intact αs - or β-caseins, total free amino acid (FAA) contents, and the ratio of hydrophilic and hydrophobic peptides in the water-soluble fraction of RMC and PMC were found...
December 2017: Food Technology and Biotechnology
Yugal Kishore Mohanta, Debasis Nayak, Kunal Biswas, Sameer Kumar Singdevsachan, Elsayed Fathi Abd Allah, Abeer Hashem, Abdulaziz A Alqarawi, Dhananjay Yadav, Tapan Kumar Mohanta
The present study demonstrates an economical and eco-friendly method for the synthesis of silver nanoparticles (AgNPs) using the wild mushroom Ganoderma sessiliforme . The synthesis of AgNPs was confirmed and the products characterized by UV-visible spectroscopy, dynamic light scattering spectroscopy and X-ray diffraction analysis. Furthermore, Fourier transform infrared spectroscopy (ATR-FTIR) analysis was performed to identify the viable biomolecules involved in the capping and active stabilization of AgNPs...
March 14, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Partha Basu, Ajay Mehta, Minish Jain, Sudeep Gupta, Rajnish V Nagarkar, Subhashini John, Robert Petit
OBJECTIVES: A global unmet medical need exists for effective treatments for persistent, recurrent, or metastatic cervical cancer, as patients have a short life expectancy. Recently, immunotherapies have shown promising survival benefits for patients with advanced forms of cancer. Axalimogene filolisbac (ADXS11-001), a Listeria monocytogenes immunotherapy with a broad effect on the immune system, is under investigation for treatment of human papillomavirus-associated cancers including cervical cancer...
March 14, 2018: International Journal of Gynecological Cancer
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