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secretagogin

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https://www.readbyqxmd.com/read/28818673/1-25-dihydroxyvitamin-d3-induces-brain-proteomic-changes-in-cuprizone-mice-during-remyelination-involving-calcium-proteins
#1
Eystein Oveland, Agnes Nystad, Frode Berven, Kjell-Morten Myhr, Øivind Torkildsen, Stig Wergeland
Dietary supplementation of vitamin D is commonly recommended to patients with multiple sclerosis. We recently found that high-dose of the hormonally active 1,25-dihydroxyvitamin-D3 (1,25D) promotes myelin repair in the cuprizone model for de- and remyelination. In the present study, we quantified 5062 proteins, of which 125 were differentially regulated in brain tissue from 1,25D treated mice during remyelination, compared to placebo. Proteins upregulated in the early remyelination phase were involved in calcium binding, e...
August 14, 2017: Neurochemistry International
https://www.readbyqxmd.com/read/28765527/calcium-ion-induced-structural-changes-promote-dimerization-of-secretagogin-which-is-required-for-its-insulin-secretory-function
#2
Jae-Jin Lee, Seo-Yun Yang, Jimin Park, James E Ferrell, Dong-Hae Shin, Kong-Joo Lee
Secretagogin (SCGN), a hexa EF-hand calcium binding protein, plays key roles in insulin secretion in pancreatic β-cells. It is not yet understood how the binding of Ca(2+) to human SCGN (hSCGN) promotes secretion. Here we have addressed this question, using mass spectrometry combined with a disulfide searching algorithm DBond. We found that the binding of Ca(2+) to hSCGN promotes the dimerization of hSCGN via the formation of a Cys193-Cys193 disulfide bond. Hydrogen/deuterium exchange mass spectrometry (HDX-MS) and molecular dynamics studies revealed that Ca(2+) binding to the EF-hands of hSCGN induces significant structural changes that affect the solvent exposure of N-terminal region, and hence the redox sensitivity of the Cys193 residue...
August 1, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28637794/a-trpv1-to-secretagogin-regulatory-axis-controls-pancreatic-%C3%AE-cell-survival-by-modulating-protein-turnover
#3
Katarzyna Malenczyk, Fatima Girach, Edit Szodorai, Petter Storm, Åsa Segerstolpe, Giuseppe Tortoriello, Robert Schnell, Jan Mulder, Roman A Romanov, Erzsébet Borók, Fabiana Piscitelli, Vincenzo Di Marzo, Gábor Szabó, Rickard Sandberg, Stefan Kubicek, Gert Lubec, Tomas Hökfelt, Ludwig Wagner, Leif Groop, Tibor Harkany
Ca(2+)-sensor proteins are generally implicated in insulin release through SNARE interactions. Here, secretagogin, whose expression in human pancreatic islets correlates with their insulin content and the incidence of type 2 diabetes, is shown to orchestrate an unexpectedly distinct mechanism. Single-cell RNA-seq reveals retained expression of the TRP family members in β-cells from diabetic donors. Amongst these, pharmacological probing identifies Ca(2+)-permeable transient receptor potential vanilloid type 1 channels (TRPV1) as potent inducers of secretagogin expression through recruitment of Sp1 transcription factors...
July 14, 2017: EMBO Journal
https://www.readbyqxmd.com/read/28492151/secretagogin-scgn-plasma-levels-and-their-association-with-cognitive-and-social-behavior-in-children-with-autism-spectrum-disorder-asd
#4
Abdulrahman Mohammed Alhowikan, Laila Al -Ayadhi, Dost Muhammad Halepoto
OBJECTIVE: To investigate the secretagogin (SCGN) plasma levels in children with autism spectrum disorder (ASD) compared to age and gender-matched healthy control, and its association with cognitive and social behaviors by using childhood autism rating scale (CARS) and social responsiveness scale (SRS). STUDY DESIGN: Case-control study. PLACE AND DURATION OF STUDY: Autism Research and Treatment Center, Al-Amodi Autism Research Chair, Department of Physiology, Faculty of Medicine, King Khalid University Hospital, King Saud University, Riyadh, Saudi Arabia, from October 2015 to May 2016...
April 2017: Journal of the College of Physicians and Surgeons—Pakistan: JCPSP
https://www.readbyqxmd.com/read/28449024/secretagogin-is-expressed-by-developing-neocortical-gabaergic-neurons-in-humans-but-not-mice-and-increases-neurite-arbor-size-and-complexity
#5
Chandrasekhar S Raju, Julien Spatazza, Amelia Stanco, Phillip Larimer, Shawn F Sorrells, Kevin W Kelley, Cory R Nicholas, Mercedes F Paredes, Jan H Lui, Andrea R Hasenstaub, Arnold R Kriegstein, Arturo Alvarez-Buylla, John L Rubenstein, Michael C Oldham
The neocortex of primates, including humans, contains more abundant and diverse inhibitory neurons compared with rodents, but the molecular foundations of these observations are unknown. Through integrative gene coexpression analysis, we determined a consensus transcriptional profile of GABAergic neurons in mid-gestation human neocortex. By comparing this profile to genes expressed in GABAergic neurons purified from neonatal mouse neocortex, we identified conserved and distinct aspects of gene expression in these cells between the species...
April 26, 2017: Cerebral Cortex
https://www.readbyqxmd.com/read/28373392/deafferented-adult-rod-bipolar-cells-create-new-synapses-with-photoreceptors-to-restore-vision
#6
Corinne Beier, Anahit Hovhannisyan, Sydney Weiser, Jennifer Kung, Seungjun Lee, Dae Yeong Lee, Philip Huie, Roopa Dalal, Daniel Palanker, Alexander Sher
Upon degeneration of photoreceptors in the adult retina, interneurons, including bipolar cells, exhibit a plastic response leading to their aberrant rewiring. Photoreceptor reintroduction has been suggested as a potential approach to sight restoration, but the ability of deafferented bipolar cells to establish functional synapses with photoreceptors is poorly understood. Here we use photocoagulation to selectively destroy photoreceptors in adult rabbits while preserving the inner retina. We find that rods and cones shift into the ablation zone over several weeks, reducing the blind spot at scotopic and photopic luminances...
April 26, 2017: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
https://www.readbyqxmd.com/read/28325894/sumoylation-and-calcium-control-syntaxin-1a-and-secretagogin-sequestration-by-tomosyn-to-regulate-insulin-exocytosis-in-human-%C3%A3-cells
#7
Mourad Ferdaoussi, Jianyang Fu, Xiaoqing Dai, Jocelyn E Manning Fox, Kunimasa Suzuki, Nancy Smith, Gregory Plummer, Patrick E MacDonald
Insulin secretion from pancreatic ß cells is a multistep process that requires the coordination of exocytotic proteins that integrate diverse signals. These include signals derived from metabolic control of post-translational SUMOylation and depolarization-induced rises in intracellular Ca(2+). Here we show that tomosyn, which suppresses insulin exocytosis by binding syntaxin1A, does so in a manner which requires its SUMOylation. Glucose-dependent de-SUMOylation of tomosyn1 at K298 releases syntaxin1A and controls the amplification of exocytosis in concert with a recently-identified tomosyn1-interacting partner; the Ca(2+)-binding protein secretagogin, which dissociates from tomosyn1 in response to Ca(2+)-raising stimuli and is required for insulin granule trafficking and exocytosis downstream of Ca(2+) influx...
March 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28223495/secretagogin-dependent-matrix-metalloprotease-2-release-from-neurons-regulates-neuroblast-migration
#8
János Hanics, Edit Szodorai, Giuseppe Tortoriello, Katarzyna Malenczyk, Erik Keimpema, Gert Lubec, Zsófia Hevesi, Mirjam I Lutz, Márk Kozsurek, Zita Puskár, Zsuzsanna E Tóth, Ludwig Wagner, Gábor G Kovács, Tomas G M Hökfelt, Tibor Harkany, Alán Alpár
The rostral migratory stream (RMS) is viewed as a glia-enriched conduit of forward-migrating neuroblasts in which chemorepulsive signals control the pace of forward migration. Here we demonstrate the existence of a scaffold of neurons that receive synaptic inputs within the rat, mouse, and human fetal RMS equivalents. These neurons express secretagogin, a Ca(2+)-sensor protein, to execute an annexin V-dependent externalization of matrix metalloprotease-2 (MMP-2) for reconfiguring the extracellular matrix locally...
March 7, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28193530/calcium-binding-protein-secretagogin-characterizes-novel-groups-of-interneurons-in-the-rat-striatum
#9
Toshio Kosaka, Seiko Yasuda, Katsuko Kosaka
In the rat striatum numerous secretagogin (SCGN) positive neurons were scattered. They were heterogeneous in their morphological and chemical properties. We examined the colocalization of SCGN with known four interneuron markers, parvalbumin (PV), calretinin (CR), nitric oxide synthase (NOS) and choline acetyl transferase (ChAT). 60-70% of SCGN positive striatal neurons contained either PV or CR or ChAT, but none contained NOS. On the other hand the remaining 30-40% expressed none of these markers, most of which were GAD positive...
February 11, 2017: Neuroscience Research
https://www.readbyqxmd.com/read/28057867/molecular-diversity-of-corticotropin-releasing-hormone-mrna-containing-neurons-in-the-hypothalamus
#10
REVIEW
Roman A Romanov, Alán Alpár, Tomas Hökfelt, Tibor Harkany
Hormonal responses to acute stress rely on the rapid induction of corticotropin-releasing hormone (CRH) production in the mammalian hypothalamus, with subsequent instructive steps culminating in corticosterone release at the periphery. Hypothalamic CRH neurons in the paraventricular nucleus of the hypothalamus are therefore considered as 'stress neurons'. However, significant morphological and functional diversity among neurons that can transiently produce CRH in other hypothalamic nuclei has been proposed, particularly as histochemical and molecular biology evidence associates CRH to both GABA and glutamate neurotransmission...
March 2017: Journal of Endocrinology
https://www.readbyqxmd.com/read/27997125/secretagogin-is-a-redox-responsive-ca-2-sensor
#11
Radhika Khandelwal, Anand Kumar Sharma, Swathi Chadalawada, Yogendra Sharma
Secretagogin (SCGN), a multifunctional, Ca(2+) binding, regulatory protein, known to regulate insulin release, has recently been implicated in the control of stress-related corticotropin-releasing hormone (CRH) secretion. Localization of SCGN to multiple intracellular (such as cytosol, nucleus, and endoplasmic reticulum) and extracellular sites appears to provide multifunctional capabilities; however, the structural elements conferring such a widespread cellular distribution to SCGN remain unidentified. We report that the spatial and functional attributes of SCGN plausibly originate from the interplay between Ca(2+) and its redox state...
January 17, 2017: Biochemistry
https://www.readbyqxmd.com/read/27812162/calcium-binding-and-disulfide-bonds-regulate-the-stability-of-secretagogin-towards-thermal-and-urea-denaturation
#12
Kalyani Sanagavarapu, Tanja Weiffert, Niamh Ní Mhurchú, David O'Connell, Sara Linse
Secretagogin is a calcium-sensor protein with six EF-hands. It is widely expressed in neurons and neuro-endocrine cells of a broad range of vertebrates including mammals, fishes and amphibia. The protein plays a role in secretion and interacts with several vesicle-associated proteins. In this work, we have studied the contribution of calcium binding and disulfide-bond formation to the stability of the secretagogin structure towards thermal and urea denaturation. SDS-PAGE analysis of secretagogin in reducing and non-reducing conditions identified a tendency of the protein to form dimers in a redox-dependent manner...
2016: PloS One
https://www.readbyqxmd.com/read/27669410/secretagogin-expression-delineates-functionally-specialized-populations-of-striatal-parvalbumin-containing-interneurons
#13
Farid N Garas, Rahul S Shah, Eszter Kormann, Natalie M Doig, Federica Vinciati, Kouichi C Nakamura, Matthijs C Dorst, Yoland Smith, Peter J Magill, Andrew Sharott
Corticostriatal afferents can engage parvalbumin-expressing (PV+) interneurons to rapidly curtail the activity of striatal projection neurons (SPNs), thus shaping striatal output. Schemes of basal ganglia circuit dynamics generally consider striatal PV+ interneurons to be homogenous, despite considerable heterogeneity in both form and function. We demonstrate that the selective co-expression of another calcium-binding protein, secretagogin (Scgn), separates PV+ interneurons in rat and primate striatum into two topographically-, physiologically- and structurally-distinct cell populations...
September 26, 2016: ELife
https://www.readbyqxmd.com/read/27095850/secretagogin-affects-insulin-secretion-in-pancreatic-%C3%AE-cells-by-regulating-actin-dynamics-and-focal-adhesion
#14
Seo-Yun Yang, Jae-Jin Lee, Jin-Hee Lee, Kyungeun Lee, Seung Hoon Oh, Yu-Mi Lim, Myung-Shik Lee, Kong-Joo Lee
Secretagogin (SCGN), a Ca(2+)-binding protein having six EF-hands, is selectively expressed in pancreatic β-cells and neuroendocrine cells. Previous studies suggested that SCGN enhances insulin secretion by functioning as a Ca(2+)-sensor protein, but the underlying mechanism has not been elucidated. The present study explored the mechanism by which SCGN enhances glucose-induced insulin secretion in NIT-1 insulinoma cells. To determine whether SCGN influences the first or second phase of insulin secretion, we examined how SCGN affects the kinetics of insulin secretion in NIT-1 cells...
June 15, 2016: Biochemical Journal
https://www.readbyqxmd.com/read/27045242/-location-and-functions-of-secretagogin-protein
#15
Qin Liu, Maode Lai
Secretagogin (SCGN) is a novel member of EF-hand Ca2+-binding proteins, which was identified in islet β cells by Wagner. SCGN is a six EF-hand Ca2+-binding protein, primarily expressed on the neuroendocrine axis and the central nervous system. The protein has abundant biological functions. A certain concentration of calcium ion can lead to conformation change of SCGN, resulting in the change of intracellular signal transduction. Preliminary studies showed that SCGN would be used to treat stress reaction, such as mental illness (depression), burns or post-traumatic stress disorder and chronic stress reaction caused by pain...
January 2016: Zhejiang da Xue Xue Bao. Yi Xue Ban, Journal of Zhejiang University. Medical Sciences
https://www.readbyqxmd.com/read/27045237/-association-of-neuroendocrine-differentiation-with-progression-and-prognosis-of-gastric-adenocarcinoma
#16
Fangying Xu, Qin Liu, Fengyan Han, Enping Xu, Maode Lai, Zhongsheng Zhao
OBJECTIVE: To investigate the association of neuroendocrine differentiation with progression and prognosis of gastric adenocarcinoma. METHODS: Clinicopathological data of 240 patients with gastric adenocarcinomas were retrospectively analyzed. The expression of chromogranin A, synaptophysin and secrectagogin in cancer tissue was assessed by immunohistochemistry. The association of neuoroendocrine differentiation parameters with disease progression and survival of patients was analyzed...
January 2016: Zhejiang da Xue Xue Bao. Yi Xue Ban, Journal of Zhejiang University. Medical Sciences
https://www.readbyqxmd.com/read/26986842/functional-analysis-of-novel-candidate-regulators-of-insulin-secretion-in-the-min6-mouse-pancreatic-%C3%AE-cell-line
#17
Masaki Kobayashi, Eiji Yamato, Koji Tanabe, Fumi Tashiro, Satsuki Miyazaki, Jun-ichi Miyazaki
Elucidating the regulation of glucose-stimulated insulin secretion (GSIS) in pancreatic β cells is important for understanding and treating diabetes. The pancreatic β cell line, MIN6, retains GSIS but gradually loses it in long-term culture. The MIN6 subclone, MIN6c4, exhibits well-regulated GSIS even after prolonged culture. We previously used DNA microarray analysis to compare gene expression in the parental MIN6 cells and MIN6c4 cells and identified several differentially regulated genes that may be involved in maintaining GSIS...
2016: PloS One
https://www.readbyqxmd.com/read/26899253/calcium-buffer-proteins-are-specific-markers-of-human-retinal-neurons
#18
Orsolya Kántor, Szilvia Mezey, Jennifer Adeghate, Angela Naumann, Roland Nitschke, Anna Énzsöly, Arnold Szabó, Ákos Lukáts, János Németh, Zoltán Somogyvári, Béla Völgyi
Ca(2+)-buffer proteins (CaBPs) modulate the temporal and spatial characteristics of transient intracellular Ca(2+)-concentration changes in neurons in order to fine-tune the strength and duration of the output signal. CaBPs have been used as neurochemical markers to identify and trace neurons of several brain loci including the mammalian retina. The CaBP content of retinal neurons, however, varies between species and, thus, the results inferred from animal models cannot be utilised directly by clinical ophthalmologists...
July 2016: Cell and Tissue Research
https://www.readbyqxmd.com/read/26199148/g-protein-gated-inwardly-rectifying-potassium-channel-subunits-1-and-2-are-down-regulated-in-rat-dorsal-root-ganglion-neurons-and-spinal-cord-after-peripheral-axotomy
#19
Chuang Lyu, Jan Mulder, Swapnali Barde, Kristoffer Sahlholm, Hugo Zeberg, Johanna Nilsson, Peter Århem, Tomas Hökfelt, Kaj Fried, Tie-Jun Sten Shi
BACKGROUND: Increased nociceptive neuronal excitability underlies chronic pain conditions. Various ion channels, including sodium, calcium and potassium channels have pivotal roles in the control of neuronal excitability. The members of the family of G protein-gated inwardly rectifying potassium (GIRK) channels, GIRK1-4, have been implicated in modulating excitability. Here, we investigated the expression and distribution of GIRK1 and GIRK2 in normal and injured dorsal root ganglia (DRGs) and spinal cord of rats...
2015: Molecular Pain
https://www.readbyqxmd.com/read/25703053/secretagogin-a-hexa-ef-hand-calcium-binding-protein-high-level-bacterial-overexpression-one-step-purification-and-properties
#20
Anand Kumar Sharma, Radhika Khandelwal, Yogendra Sharma, Vangipurapu Rajanikanth
Secretagogin (SCGN), a hexa EF-hand calcium-binding protein, is highly expressed in the endocrine cells (especially in pancreatic islets) and in restricted neuronal sub-populations, albeit at comparatively low level. Since SCGN is predicted to be a potential neuroendocrine marker in carcinoid tumors of lung and gastrointestinal tract, it is of paramount importance to understand the features of this protein in different environment for assigning its crucial functions in different tissues and under pathophysiological conditions...
May 2015: Protein Expression and Purification
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