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https://www.readbyqxmd.com/read/28532094/electrochemical-sensing-of-doxorubicin-in-unprocessed-whole-blood-cell-lysate-and-human-plasma-samples-using-thin-film-of-poly-arginine-modified-glassy-carbon-electrode
#1
Jafar Soleymani, Mohammad Hasanzadeh, Morteza Eskandani, Maryam Khoubnasabjafari, Nasrin Shadjou, Abolghasem Jouyban
A thin film of poly-arginine fabricated on glassy carbon electrode by one step electrodeposition method is applied for detection of doxorubicin hydrochloride in whole blood, cell lysate, and untreated-plasma samples. Cyclic voltammetry results indicated that the doxorubicin is oxidized via two electrons and two protons at physiological pH (pH=7.4) using poly-arginine thin film modified glassy carbon. More importantly, electrostatic repulsion takes place between the prepared polymer film-modified electrode and selected drug resulting in the signal amplification on oxidation of doxorubicin and lowering its over potential and thereby selective detection of doxorubicin in real samples...
August 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28531196/co-inheritance-of-glucose-6-phosphate-dehydrogenase-deficiency-mutations-and-hemoglobin-e-in-a-kachin-population-in-a-malaria-endemic-region-of-southeast-asia
#2
Zeshuai Deng, Fang Yang, Yao Bai, Lijun He, Qing Li, Yanrui Wu, Lan Luo, Hong Li, Limei Ma, Zhaoqing Yang, Yongshu He, Liwang Cui
Glucose-6-phosphate dehydrogenase (G6PD) deficiency and hemoglobin E (HbE, β26 Glu-Lys) are two common red cell disorders in Southeast Asia. G6PD deficiency produces hemolytic anemia, which can be triggered by certain drugs or infections. HbE is asymptomatic or is manifested as microcytic, minimally hemolytic anemia. The association between G6PD deficiency and HbE is little understood. This study aimed to investigate G6PD deficiency and HbE in a Kachin ethnic group in the China-Myanmar border area. G6PD enzyme activity was measured using a quantitative G6PD assay, G6PD variants genotyped by the SNaPshot assay, and an HbE gene mutation identified by an amplification refractory mutation system and subsequently confirmed by using a reverse dot blot hybridization assay from 100 unrelated individuals in the study area...
2017: PloS One
https://www.readbyqxmd.com/read/28531156/molecular-techniques-for-the-detection-of-organisms-in-aquatic-environments-with-emphasis-on-harmful-algal-bloom-species
#3
REVIEW
Linda K Medlin, Jahir Orozco
Molecular techniques to detect organisms in aquatic ecosystems are being gradually considered as an attractive alternative to standard laboratory methods. They offer faster and more accurate means of detecting and monitoring species, with respect to their traditional homologues based on culture and microscopic counting. Molecular techniques are particularly attractive when multiple species need to be detected and/or are in very low abundance. This paper reviews molecular techniques based on whole cells, such as microscope-based enumeration and Fluorescence In-Situ Hybridization (FISH) and molecular cell-free formats, such as sandwich hybridization assay (SHA), biosensors, microarrays, quantitative polymerase chain reaction (qPCR) and real time PCR (RT-PCR)...
May 22, 2017: Sensors
https://www.readbyqxmd.com/read/28530707/a-mili-independent-pirna-biogenesis-pathway-empowers-partial-germline-reprogramming
#4
Lina Vasiliauskaitė, Dimitrios Vitsios, Rebecca V Berrens, Claudia Carrieri, Wolf Reik, Anton J Enright, Dónal O'Carroll
In mice, the pathway involving PIWI and PIWI-interacting RNA (PIWI-piRNA) is essential to re-establish transposon silencing during male-germline reprogramming. The cytoplasmic PIWI protein MILI mediates piRNA-guided transposon RNA cleavage as well as piRNA amplification. MIWI2's binding to piRNA and its nuclear localization are proposed to be dependent upon MILI function. Here, we demonstrate the existence of a piRNA biogenesis pathway that sustains partial MIWI2 function and reprogramming activity in the absence of MILI...
May 22, 2017: Nature Structural & Molecular Biology
https://www.readbyqxmd.com/read/28530580/offgel-electrophoresis-and-tandem-mass-spectrometry-approach-compared-with-dna-based-pcr-method-for-authentication-of-meat-species-from-raw-and-cooked-ground-meat-mixtures-containing-cattle-meat-water-buffalo-meat-and-sheep-meat
#5
Basappa M Naveena, Deepak S Jagadeesh, A Jagadeesh Babu, T Madhava Rao, Veeranna Kamuni, S Vaithiyanathan, Vinayak V Kulkarni, Srikanth Rapole
The present study compared the accuracy of an OFFGEL electrophoresis and tandem mass spectrometry-based proteomic approach with a DNA-based method for meat species identification from raw and cooked ground meat mixes containing cattle, water buffalo and sheep meat. The proteomic approach involved the separation of myofibrillar proteins using OFFGEL electrophoresis, SDS-PAGE and protein identification by MALDI-TOF MS. Species-specific peptides derived from myosin light chain-1 and 2 were identified for authenticating buffalo meat spiked at a minimum 0...
October 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28530222/ultrasensitive-and-high-efficiency-screen-of-de-novo-low-frequency-mutations-by-o2n-seq
#6
Kaile Wang, Shujuan Lai, Xiaoxu Yang, Tianqi Zhu, Xuemei Lu, Chung-I Wu, Jue Ruan
Detection of de novo, low-frequency mutations is essential for characterizing cancer genomes and heterogeneous cell populations. However, the screening capacity of current ultrasensitive NGS methods is inadequate owing to either low-efficiency read utilization or severe amplification bias. Here, we present o2n-seq, an ultrasensitive and high-efficiency NGS library preparation method for discovering de novo, low-frequency mutations. O2n-seq reduces the error rate of NGS to 10(-5)-10(-8). The efficiency of its data usage is about 10-30 times higher than that of barcode-based strategies...
May 22, 2017: Nature Communications
https://www.readbyqxmd.com/read/28530022/development-of-a-reverse-transcription-quantitative-polymerase-chain-reaction-based-assay-for-broad-coverage-detection-of-african-and-asian-zika-virus-lineages
#7
Yang Yang, Gary Wong, Baoguo Ye, Shihua Li, Shanqin Li, Haixia Zheng, Qiang Wang, Mifang Liang, George F Gao, Lei Liu, Yingxia Liu, Yuhai Bi
The Zika virus (ZIKV) is an arbovirus that has spread rapidly worldwide within recent times. There is accumulating evidence that associates ZIKV infections with Guillain-Barré Syndrome (GBS) and microcephaly in humans. The ZIKV is genetically diverse and can be separated into Asian and African lineages. A rapid, sensitive, and specific assay is needed for the detection of ZIKV across various pandemic regions. So far, the available primers and probes do not cover the genetic diversity and geographic distribution of all ZIKV strains...
May 19, 2017: Virologica Sinica
https://www.readbyqxmd.com/read/28529837/fifteen-microsatellite-markers-for-herbertia-zebrina-iridaceae-an-endangered-species-from-south-american-grasslands
#8
Cristiane Forgiarini, Manuel Curto, Eudes Maria Stiehl-Alves, Christian Bräuchler, Johannes Kollmann, Harald Meimberg, Tatiana Teixeira de Souza-Chies
PREMISE OF THE STUDY: Polymorphic microsatellite loci were developed and used to genotype individuals of Herbertia zebrina (Iridaceae) as a first step for assessment of intraspecific genetic diversity. METHODS AND RESULTS: Primer pairs for 47 markers were developed: 20 from a microsatellite-enriched library and 27 from a next-generation sequencing run using the Illumina MiSeq platform. Of those, 15 loci were considered successful, of which 12 were polymorphic and three were monomorphic...
May 2017: Applications in Plant Sciences
https://www.readbyqxmd.com/read/28529836/chloroplast-and-mitochondrial-microsatellites-for-millettia-pinnata-fabaceae-and-cross-amplification-in-related-species
#9
Yanling Wang, Hongxian Xie, Yi Yang, Yelin Huang, Jianwu Wang, Fengxiao Tan
PREMISE OF THE STUDY: Chloroplast and mitochondrial microsatellites were identified to study the population genetics of Millettia pinnata (Fabaceae). METHODS AND RESULTS: Based on publicly available plastid genome sequence data of M. pinnata, 42 primer pairs were developed, of which 17 displayed polymorphisms across 89 individuals from four populations. For chloroplast loci, two to six alleles were recovered and the unbiased haploid diversity per locus ranged from 0...
May 2017: Applications in Plant Sciences
https://www.readbyqxmd.com/read/28529835/a-new-set-of-microsatellite-primers-for-coelogyne-fimbriata-orchidaceae-and-cross-amplification-in-c-ovalis
#10
Wei-Chang Huang, Kai Jiang, Chao Hu, Yue-E Xiao, Barnabas C Seyler, Yuan-Yuan Li
PREMISE OF THE STUDY: Declining orchid populations have made it necessary to prioritize the study of population structure and genetic diversity for species including Coelogyne fimbriata (Orchidaceae). METHODS AND RESULTS: A biotin-streptavidin capture method was used to construct a microsatellite library for C. fimbriata. A total of 15 polymorphic nuclear microsatellite loci were isolated and characterized using 47 C. fimbriata individuals from two natural populations in China...
May 2017: Applications in Plant Sciences
https://www.readbyqxmd.com/read/28529833/development-and-evaluation-of-microsatellite-markers-for-the-critically-endangered-birch-betula-chichibuensis-betulaceae
#11
Yuji Igarashi, Hiroki Aihara, Yoshihiro Handa, Hiroshi Katsumata, Masanori Fujii, Koichiro Nakano, Toshihide Hirao
PREMISE OF THE STUDY: Microsatellite markers were developed and characterized for the critically endangered birch Betula chichibuensis (Betulaceae) to investigate the genetic structure of this species for conservation purposes. METHODS AND RESULTS: Sixteen microsatellite markers with di-, tri-, and tetranucleotide repeat motifs were developed and optimized using MiSeq paired-end sequencing. Of these, 14 were polymorphic, with two to five alleles per locus, in 47 individuals from two newly discovered populations of B...
May 2017: Applications in Plant Sciences
https://www.readbyqxmd.com/read/28529740/copy-number-variation-and-protein-expression-of-dot1l-in-pancreatic-adenocarcinoma-as-a-potential-drug-target
#12
Heike Loeser, Dirk Waldschmidt, Fabian Kuetting, Carina Heydt, Thomas Zander, Patrick Plum, Hakan Alakus, Reinhard Buettner, Alexander Quaas
Adenocarcinoma of the pancreas has a poor prognosis. At present, no relevant personalized targets have been identified. Sequencing studies have implicated gene alterations of disruptor of telomeric silencing 1 like histone lysine methyltransferase (DOT1L) in pancreatic adenocarcinoma. DOT1L is part of the histone modification system and catalyzes methylation of H3K79, which is crucial in cell signaling and DNA damage repair. DOT1L is considered to be a target of therapy in mixed lineage leukemia gene-deficient leukemia cases and a potential target in breast carcinoma...
May 2017: Molecular and Clinical Oncology
https://www.readbyqxmd.com/read/28529729/characterization-of-the-novel-in1059-harbouring-vim-gene-cassette
#13
Dongguo Wang, Jinhong Yang, Meiyu Fang, Wei He, Ying Zhang, Caixia Liu, Dongsheng Zhou
BACKGROUND: VIM-type enzyme encodes the most widely acquired metallo-β-lactamases in Gram- negative bacteria. To obtain current epidemiological data for integrons from enterobacteriae in hospital, the study characterizes the genetic structure in In1059 by comparison with In846 integrons harbouring VIM gene and other class 1 integrons including In37, In62, In843 and In1021 with the aim of identifying the putative mechanisms involved integron mobilization and infer evolution of relevant integrons...
2017: Antimicrobial Resistance and Infection Control
https://www.readbyqxmd.com/read/28529717/gene-length-and-detection-bias-in-single-cell-rna-sequencing-protocols
#14
Belinda Phipson, Luke Zappia, Alicia Oshlack
Background: Single cell RNA sequencing (scRNA-seq) has rapidly gained popularity for profiling transcriptomes of hundreds to thousands of single cells. This technology has led to the discovery of novel cell types and revealed insights into the development of complex tissues. However, many technical challenges need to be overcome during data generation. Due to minute amounts of starting material, samples undergo extensive amplification, increasing technical variability. A solution for mitigating amplification biases is to include unique molecular identifiers (UMIs), which tag individual molecules...
2017: F1000Research
https://www.readbyqxmd.com/read/28529628/cross-platform-comparison-of-four-leading-technologies-for-detecting-egfr-mutations-in-circulating-tumor-dna-from-non-small-cell-lung-carcinoma-patient-plasma
#15
Ting Xu, Xiaozheng Kang, Xiaofang You, Liang Dai, Dequan Tian, Wanpu Yan, Yongbo Yang, Hongchao Xiong, Zhen Liang, Grace Q Zhao, Shengrong Lin, Ke-Neng Chen, Guobing Xu
Analysis of circulating tumor DNA (ctDNA) is emerging as a powerful tool for guiding targeted therapy and monitoring tumor evolution in patients with non-small cell lung cancer (NSCLC), especially when representative tissue biopsies are not available. Here, we have compared the ability of four leading technology platforms to detect epidermal growth factor receptor (EGFR) mutations (L858R, exon 19 deletion, T790M and G719X) in ctDNA from NSCLC patients. Two amplification refractory mutation systems (cobas-ARMS and ADx-ARMS), a droplet digital polymerase chain reaction (ddPCR) and a next-generation sequencing (Firefly NGS) platform were included in the comparison...
2017: Theranostics
https://www.readbyqxmd.com/read/28528784/keeping-the-voice-fit-in-the-group-fitness-industry-a-qualitative-study-to-determine-what-instructors-want-in-a-voice-education-program
#16
Patrick J Aiken, Anna F Rumbach
OBJECTIVES: This study aimed to provide a descriptive summary of (1) group fitness instructors' (GFIs') experiences of occupational voice use and education, and (2) the content and mode of delivery desired by GFIs in an education and training program. STUDY DESIGN: This is a qualitative inductive approach using a semi-structured interview. METHOD: Semi-structured interviews were conducted with eight GFIs recruited via self-selection sampling...
May 18, 2017: Journal of Voice: Official Journal of the Voice Foundation
https://www.readbyqxmd.com/read/28528511/met-overexpression-and-gene-amplification-prevalence-clinico-pathological-characteristics-and-prognostic-significance-in-a-large-cohort-of-patients-with-surgically-resected-nsclc
#17
William Sterlacci, Michael Fiegl, Mathias Gugger, Lukas Bubendorf, Spasenija Savic, Alexandar Tzankov
The prevalence of overexpression and amplification of the proto-oncogene mesenchymal epithelial transition (MET) in non-small cell lung cancer (NSCLC) varies greatly in the literature. Since MET is a potential treatment target, knowledge of its prevalence and prognostic importance is crucial. We investigated MET expression and gene status in 735 NSCLC cases using tissue microarrays. Prognostic significance as well as correlations with various clinico-pathological parameters were evaluated. The prevalence of MET overexpression was 17% and MET amplification was present in 2...
May 20, 2017: Virchows Archiv: An International Journal of Pathology
https://www.readbyqxmd.com/read/28528450/high-expression-of-cyclin-d1-is-associated-to-high-proliferation-rate-and-increased-risk-of-mortality-in-women-with-er-positive-but-not-in-er-negative-breast-cancers
#18
Cecilia Ahlin, Claudia Lundgren, Elin Embretsén-Varro, Karin Jirström, Carl Blomqvist, M -L Fjällskog
PURPOSE: Cyclin D1 has a central role in cell cycle control and is an important component of estrogen regulation of cell cycle progression. We have previously shown that high cyclin D expression is related to aggressive features of ER-positive but not ER-negative breast cancer. The aims of the present study were to validate this differential ER-related effect and furthermore explore the relationship between cyclin D overexpression and CCND1 gene amplification status in a node-negative breast cancer case-control study...
May 20, 2017: Breast Cancer Research and Treatment
https://www.readbyqxmd.com/read/28527919/mechanisms-of-the-amplifying-pathway-of-insulin-secretion-in-the-%C3%AE-cell
#19
REVIEW
Michael A Kalwat, Melanie Cobb
Pancreatic islet β cells secrete insulin in response to nutrient secretagogues, like glucose, dependent on calcium influx and nutrient metabolism. One of the most intriguing qualities of β cells is their ability to use metabolism to amplify the amount of secreted insulin independent of further alterations in intracellular calcium. Many years studying this amplifying process have shaped our current understanding of β cell stimulus-secretion coupling; yet, the exact mechanisms of amplification have been elusive...
May 17, 2017: Pharmacology & Therapeutics
https://www.readbyqxmd.com/read/28527895/suppression-of-pmrab11-inhibits-yhv-infection-in-penaeus-monodon
#20
Akechai Kongprajug, Sakol Panyim, Chalermporn Ongvarrasopone
Yellow head virus (YHV) is one of the most serious pathogens that causes worldwide shrimp production loss. It enters the cells via clathrin-mediated endocytosis and utilizes small GTPase Rab proteins such as PmRab5 and PmRab7 for intracellular trafficking. In this study, molecular cloning and functional analysis of Rab11 during YHV infection were investigated. PmRab11 cDNA was cloned by Rapid amplification of cDNA ends (RACEs). It contained two forms of sizes 1200 and 1050 bp distinct at the 5' UTR. The coding region of PmRab11 was 645 bp, encoding 214 amino acids...
May 17, 2017: Fish & Shellfish Immunology
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