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https://www.readbyqxmd.com/read/29924741/a-hybridization-chain-reaction-amplification-strategy-for-fluorescence-imaging-of-human-telomerase-activity-in-living-cells
#1
Zhiqian Zhang, Changli Zhong, Taixian Yuan, Xiaoyan Zhou, Min Zhao, Husun Qian, Wei Cheng, Tingmei Chen
A hybridized chain reaction (HCR)-based biosensing method has been developed for the imaging detection of intracellular telomerase activity. The telomerase-targeting responder-transmitter DNA complex (HPT) consisting of telomerase primer sequence (HP) and a HCR initiator (trigger) is transfected into cell plasma. In the presence of telomerase, HPT can be recognized and extended, producing plenty of triggers which initiates HCR amplification reaction. Finally, a long nicked dsDNA with a lot of outstretched single chains was formed by hybridizing with Q of the reporter complex, generating an enhanced fluorescence signal...
June 20, 2018: Methods and Applications in Fluorescence
https://www.readbyqxmd.com/read/29924598/an-ultrasensitive-diagnostic-biochip-based-on-biomimetic-periodic-nanostructure-assisted-rolling-circle-amplification
#2
Qian Yao, Yingqian Wang, Jie Wang, Shaomin Chen, Haoyang Liu, Zhuoran Jiang, Xiaoe Zhang, Song-Mei Liu, Quan Yuan, Xiang Zhou
Developing portable and sensitive devices for point of care (POC) detection of low abundance bioactive molecules is highly valuable in early diagnosis of disease. Herein, an ultrasensitive photonic crystal-assisted rolling circle amplification (PC-RCA) biochip was constructed and further applied to serum circulating microRNAs (miRNAs) detection. The biochip integrates the optical signal enhancement capability of biomimetic PC surface with the thousand-fold signal amplification feature of RCA. The biomimetic PC displayed periodic dielectric nanostructure and significantly enhanced the signal intensity of RCA reaction, leading to efficient improvement of detection sensitivity...
June 20, 2018: ACS Nano
https://www.readbyqxmd.com/read/29924359/identification-of-aspergillus-and-mucorales-in-formalin-fixed-paraffin-embedded-tissue-samples-comparison-of-specific-and-broad-range-fungal-qpcr-assays
#3
J Springer, I McCormick Smith, S Hartmann, R Winkelmann, D Wilmes, O Cornely, J Kessel, J Löffler, V Rickerts
Establishing the etiology of invasive fungal infections is important to guide therapeutic options and for epidemiologic purposes. Formalin-fixed, paraffin-embedded (FFPE) tissue specimens from patients with proven invasive fungal infections are valuable to determine the etiology of systemic fungal infections. We compared different polymerase chain reaction (PCR) amplification strategies from FFPE tissue blocks to identify agents of invasive fungal infections. We found that specific PCR assays show superior sensitivity in the identification of DNA of Mucorales and Aspergillus and mixed infections caused by both as compared to broad-range PCR assays...
June 19, 2018: Medical Mycology: Official Publication of the International Society for Human and Animal Mycology
https://www.readbyqxmd.com/read/29924355/analysis-of-a-genetic-factors-contributing-to-feathering-phenotype-in-chickens
#4
Xiuling Zhang, Han Wang, Lechao Zhang, Qi Wang, Xiaolong Du, Linhan Ge, Rongyan Zhou, Lanhui Li, Xianglong Li
In the current study, we sought to determine whether or not the endogenous retroviral ev21 influences feathering type of chickens, and if one mutation locus in the unoccupied repeat (UR) region can be used to predict the corresponding feathering type and genotype. The distribution of ev21 as well as the mutation locus in UR and occupied site (OR) regions was detected in HY-line gray progenitor (HYGP) 4 lines, HY-line brown (HYB) and Taihang chickens (TH). Furthermore, a detection method for the genotype resulting in late feathering (LF) phenotype was developed by double PCR using C line of HYGP, C line of Dawu progenitor, commercial line of HY-line gray (HYG) males, LF males of TH and Bashang long-tail chickens (BS)...
June 19, 2018: Poultry Science
https://www.readbyqxmd.com/read/29924345/recurrent-amplification-of-the-heterochromatin-protein-1-hp1-gene-family-across-dipter
#5
Quentin Helleu, Mia T Levine
The heterochromatic genome compartment mediates strictly conserved cellular processes such as chromosome segregation, telomere integrity, and genome stability. Paradoxically, heterochromatic DNA sequence is wildly unconserved. Recent reports that many hybrid incompatibility genes encode heterochromatin proteins, together with the observation that inter-species hybrids suffer aberrant heterochromatin-dependent processes, suggest that heterochromatic DNA packaging requires species-specific innovations. Testing this model of co-evolution between fast-evolving heterochromatic DNA and its packaging proteins begins with defining the latter...
June 19, 2018: Molecular Biology and Evolution
https://www.readbyqxmd.com/read/29924282/-polymorphisms-of-the-arsenite-methyltransferase-as3mt-gene-and-urinary-efficiency-of-arsenic-metabolism-in-a-population-in-northern-mexico
#6
Francisco Javier García-Alvarado, Honorio Neri-Meléndez, Luz Pérez Armendáriz, Mario Rivera Guillen
The aim of this study was to assess the relationship of the rs11191439 and rs3740393 polymorphisms of the Arsenite Methyltransferase (As3MT) gene with the arsenic urinary excretion profiles in a population in northern Mexico. A crosssectional study was conducted with 102 participants between the ages of 14 and 75 in Comarca Lagunera, Mexico. Speciation of the arsenic urinary metabolites was determined, and DNA was extracted; analysis and amplification of the polymorphisms was made by PCR in real time. A descriptive analysis was carried out and the differences in the urinary arsenic concentrations were assessed according to sex and genotype...
January 2018: Revista Peruana de Medicina Experimental y Salud Pública
https://www.readbyqxmd.com/read/29923972/rapid-detection-of-mycoplasma-pneumoniae-by-loop-mediated-isothermal-amplification-assay
#7
Xin Yuan, Changqing Bai, Qian Cui, Han Zhang, Jing Yuan, Kaiwen Niu, Yuzhong Feng, Xin Jin, Puyuan Li, Huiying Liu
To improve the diagnosis and treatment of Mycoplasma pneumoniae (Mp) infection and reduce the misuse of antibiotics, we sought to establish a loop-mediated isothermal amplification (LAMP) assay for rapid detection of Mp.Six primers specific for the Mp P1 gene were designed, and the LAMP method was used to rapidly detect Mp. The sensitivity of the LAMP method was determined by serial dilution of the standard Mp strain FH (standard strains of Mycoplasma pneumoniae). Specificity was assessed with 17 common pathogenic microorganisms in the respiratory tract...
June 2018: Medicine (Baltimore)
https://www.readbyqxmd.com/read/29923892/how-small-differences-in-assessed-clinical-performance-amplify-to-large-differences-in-grades-and-awards-a-cascade-with-serious-consequences-for-students-underrepresented-in-medicine
#8
Arianne Teherani, Karen E Hauer, Alicia Fernandez, Talmadge E King, Catherine Lucey
While students entering medical schools are becoming more diverse, trainees in residency programs in competitive specialties and academic medicine faculty have not increased in diversity. As part of an educational continuous quality improvement (ECQI) process at the University of California, San Francisco, School of Medicine, the authors examined data for the classes of 2013-2016 to determine whether differences existed between underrepresented in medicine (UIM) and not-UIM students' clinical performance (clerkship director ratings and number of clerkship honors grades awarded) and honor society membership--all of which influence residency selection and academic career choices...
June 19, 2018: Academic Medicine: Journal of the Association of American Medical Colleges
https://www.readbyqxmd.com/read/29923578/a-rapid-and-eco-friendly-isothermal-amplification-microdevice-for-multiplex-detection-of-foodborne-pathogens
#9
Thi Ngoc Diep Trinh, Nae Yoon Lee
In this study, a plastic microdevice based on loop-mediated isothermal amplification (LAMP) was fabricated for the amplification and on-chip fluorescence detection of multiple pathogens. Papers infused with LAMP reagents and specific primers were embedded inside the multiple reaction chambers of the microdevice. A solution containing the target pathogens was injected into the sample chamber, located in the center of the microdevice, and evenly distributed to the reaction chambers simultaneously via centrifugal force...
June 20, 2018: Lab on a Chip
https://www.readbyqxmd.com/read/29923519/evaluation-of-real-time-reverse-transcription-loop-mediated-isothermal-amplification-assay-for-clinical-diagnosis-of-west-nile-virus-in-patients
#10
Jyoti S Kumar, Divyasha Saxena, Manmohan Parida, Sivakumar Rathinam
Background & objectives: West Nile virus (WNV) is a mosquito-borne flavivirus. The disease can be diagnosed by isolation followed by fluorescent antibody tests, enzyme-linked immunosorbent assay and polymerase chain reaction (PCR) assay. These diagnostic methods are laborious and time-consuming. The present study was aimed to evaluate the real-time reverse-transcription loop-mediated isothermal amplification (RT-LAMP) method for rapid, early and accurate diagnosis of WNV. Methods: A one-step single tube accelerated quantitative RT-LAMP assay was evaluated by targeting the Env gene of WNV...
March 2018: Indian Journal of Medical Research
https://www.readbyqxmd.com/read/29923492/molecular-diagnostic-testing-of-diffuse-gliomas-in-the-real-life-setting-a-practical-approach
#11
Michał Bieńkowski, Adelheid Wöhrer, Patrizia Moser, Melitta Kitzwögerer, Gerda Ricken, Thomas Ströbel, Johannes A Hainfellner
Typing of diffuse gliomas according to the WHO 2016 Classification of Tumors of the Central Nervous System is based on the integration of histology with molecular biomarkers. However, the choice of appropriate methods for molecular analysis and criteria for interpretation of test results is left to each diagnostic laboratory. In the present study, we tested the applicability of combined immunohistochemistry, direct sequencing, and multiplex ligation-dependent probe amplification (MLPA) for diagnostic assessment of <i>IDH1/2</i> mutation status, chromosome 1p/19q status, and <i>TERT</i> promoter mutations...
June 20, 2018: Clinical Neuropathology
https://www.readbyqxmd.com/read/29923381/-efficiency-analysis-of-ex16-10y-kit-on-detection-of-the-uygur-population-in-xinjiang-province
#12
G Bi, C Zhang, Y Dong, H T Jiao, L Dong, H G Zhou
OBJECTIVES: To analyse the efficiency of EX16+10Y kit on the forensic detection of the Uygur in Xinjiang province. METHODS: The blood samples were extracted from 4 620 male individuals of Uygur in Xinjiang province, and amplified by EX16+10Y kit. The typing of amplification products was performed by 3130 xl genetic analyzer. RESULTS: The genotyping graphs of 15 autosomal STR loci and 10 Y-chromosomal STR loci from 4 620 male individuals of Uygur in Xinjiang province were acquired completely...
April 2018: Fa Yi Xue za Zhi
https://www.readbyqxmd.com/read/29923377/-establishment-of-dna-genetic-marker-identification-system-for-plant-evidence
#13
H Li, P Xia, C Wang, Y Wang, X Y Zhao, K Ma, W Dai, Y Cao, H G Zhou, W B Liu
OBJECTIVES: To establish a species identification system based on DNA genetic markers for plant evidence. METHODS: Two hundred common plants in Shanghai were collected and identified by morphological characteristics. The primers of gene segments rbcL , matK , and ITS were designed and amplified. The PCR amplicon was detected by agarose gel electrophoresis. After the sequencing, the universality and the identification capacity of the three markers were evaluated...
April 2018: Fa Yi Xue za Zhi
https://www.readbyqxmd.com/read/29923329/identification-of-differential-expressed-lncrnas-in-human-thyroid-cancer-by-a-genome-wide-analyses
#14
Wei Lu, Yongcan Xu, Jiewei Xu, Zhong Wang, Guochao Ye
Recently, a growing number of evidence has revealed that long noncoding RNAs (lncRNAs) act as key regulators in various cellular biologic processes, and dysregulation of lncRNAs involves in tumorigenesis and cancer progression. However, the expression pattern, clinical relevance, and biologic function of most lncRNAs in human thyroid cancer remain unclear. To identify more thyroid-cancer-associated lncRNAs, we analyzed the expression profile of lncRNAs in thyroid cancer tissues and adjacent normal or non-tumor tissues using RNA sequencing data and gene microarray data from The Cancer Genome Atlas and Gene Expression Omnibus...
June 20, 2018: Cancer Medicine
https://www.readbyqxmd.com/read/29923182/profiles-of-brain-metastases-prioritization-of-therapeutic-targets
#15
Sherise D Ferguson, Siyuan Zheng, Joanne Xiu, Shouhao Zhou, Mustafa Khasraw, Priscilla K Brastianos, Santosh Kesari, Jethro Hu, Jeremy Rudnick, Michael E Salacz, David Piccioni, Suyun Huang, Michael A Davies, Isabella C Glitza, John V Heymach, Jianjun Zhang, Nuhad K Ibrahim, John F DeGroot, Joseph McCarty, Barbara J O'Brien, Raymond Sawaya, Roeland G W Verhaak, Sandeep K Reddy, Waldemar Priebe, Zoran Gatalica, David Spetzler, Amy B Heimberger
We sought to compare the tumor profiles of brain metastases from common cancers with those of primary tumors and extracranial metastases in order to identify potential targets and prioritize rational treatment strategies. Tumor samples were collected from both the primary and metastatic sites of non-small cell lung cancer, breast cancer, and melanoma from patients in locations worldwide, and these were submitted to Caris Life Sciences for tumor multiplatform analysis, including gene sequencing (Sanger and next-generation sequencing with a targeted 47-gene panel), protein expression (assayed by immunohistochemistry), and gene amplification (assayed by in situ hybridization)...
June 19, 2018: International Journal of Cancer. Journal International du Cancer
https://www.readbyqxmd.com/read/29923135/attenuation-of-pulse-pressure-amplification-in-patients-with-complete-transposition-of-the-great-arteries-after-an-arterial-switch-operation-in-children
#16
Tomoaki Murakami, Masahiro Shiraishi, Taro Murakami, Atsuhito Takeda
Although the arterial switch operation has become the standard procedure for infants with complete transposition of the great arteries, possible late adverse events after surgery have not been fully elucidated. One such problem may be the postoperative function of the aorta that is radically manipulated. The current study enrolled 12 patients aged 4-9 years who had undergone an arterial switch operation. The ascending and descending aortic pressure waveforms were recorded by a catheter-mounted pressure sensor...
June 19, 2018: Pediatric Cardiology
https://www.readbyqxmd.com/read/29922862/a-molecularly-imprinted-sensor-with-enzymatic-enhancement-of-electrochemiluminescence-of-quantum-dots-for-ultratrace-clopyralid-determination
#17
Qingyu Wang, Shuhuai Li, Jianping Li
A new molecularly imprinted polymer electrochemiluminescence (ECL) sensor was developed for the detection of clopyralid (CPD) based on enzyme-biocatalyzed amplification. CdTe quantum dots were immobilized on the surface of an electrode by reaction with p-aminothiophenol preadsorbed on the electrode. Then a molecularly imprinted film was formed by electrochemical polymerization of o-phenylenediamine in the presence of CPD on the CdTe-modified gold electrode. During the analytical cycle, horseradish peroxidase (HRP)-labeled CPD was replaced by CPD in the sample...
June 19, 2018: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/29922617/role-of-rs4986790-polymorphism-of-tlr4-gene-in-susceptibility-towards-malaria-infection-in-the-pakistani-population
#18
Asima Rani, Syed Kashif Nawaz, Muhammad Arshad, Shazia Irfan
Background: Toll-like receptors (TLRs) of the human immune mechanism play important role in the detection of invading pathogens. TLRs specifically recognize the pathogen-associated molecular patterns (PAMPs) from pathogens and start the effective response. Single nucleotide polymorphisms (SNPs) in the TLRs can mediate their functions. Present study evaluated the importance of rs4986790 polymorphism of TLR4 gene in susceptibility towards malaria, clinical outcomes of the disease and responsible species of malaria...
May 2018: Iranian Journal of Public Health
https://www.readbyqxmd.com/read/29922412/a-prospective-cross-sectional-study-of-detection-of-clostridium-difficile-toxin-in-patients-with-antibiotic-associated-diarrhoea
#19
Arun Sachu, Kavitha Dinesh, Ismail Siyad, Anil Kumar, Anu Vasudevan, Shamsul Karim
Background and Objectives: Clostridium difficile infections (CDI) include self-limiting antibiotic associated diarrhoea (AAD), antibiotic-associated colitis, and pseudomembranous colitis. The present study aimed at detecting C. difficile toxin in stool samples of patients with AAD and analyzing the antibiotic use and presence of other risk factors in these patients. Materials and Methods: In this study, which was conducted on 660 samples, a 2- step strategy was used...
February 2018: Iranian Journal of Microbiology
https://www.readbyqxmd.com/read/29922320/jasmonic-acid-mediated-aliphatic-glucosinolate-metabolism-is-involved-in-clubroot-disease-development-in-brassica-napus-l
#20
Li Xu, Huan Yang, Li Ren, Wang Chen, Lijiang Liu, Fan Liu, Lingyi Zeng, Ruibin Yan, Kunrong Chen, Xiaoping Fang
Glucosinolate (GSL) is associated with clubroot disease, which is caused by the obligate biotrophic protist Plasmodiophora brassicae . Due to the complicated composition of GSLs, their exact role in clubroot disease development remains unclear. By investigating clubroot disease resistance in cruciferous plants and characterizing the GSL content in seeds, we can determine if clubroot disease development is related to the components of GSLs. The difference in the infection process between Matthiola incana L. (resistant) and Brassica napus L...
2018: Frontiers in Plant Science
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