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https://www.readbyqxmd.com/read/27936519/a-computational-approach-for-studying-antibody-antigen-interactions-without-prior-structural-information-the-anti-testosterone-binding-antibody-as-a-case-study
#1
Artturi Koivuniemi, Kristiina Takkinen, Tarja Nevanen
Given the increasing exploitation of antibodies in different contexts such as molecular diagnostics and therapeutics, it would be beneficial to unravel the atomistic level properties of antibody-antigen complexes with the help of computational modeling. Thus, here we have studied the feasibility of computational tools to gather atomic scale information regarding the antibody-antigen complexes solely starting from an amino acid sequence. First, we constructed a homology model for the anti-testosterone binding antibody based on the knowledge based classification of complementary determining regions (CDRs) and implicit solvent molecular dynamics simulations...
December 9, 2016: Proteins
https://www.readbyqxmd.com/read/27936517/-novel-biomarkers-for-the-assessment-of-pediatric-systemic-lupus-erythematosus-nephritis
#2
Artemis Koutsonikoli, Maria Trachana, Evangelia Farmaki, Vasiliki Tzimouli, Polyxeni Pratsidou-Gertsi, Nikoleta Printza, Alexandros Garyphallos, Vasiliki Galanopoulou, Florence Kanakoudi-Tsakalidou, Fotios Papachristou
OBJECTIVES: The discovery of serum biomarkers specific for pediatric Lupus Nephritis (pLN) will facilitate the non-invasive diagnosis, follow-up and more appropriate use of treatment. The aim of this study was to explore the role of serum High-Mobility-Group-Box-1 (HMGB1) protein, antibodies against nucleosomes (anti-NCS), complement factor C1q (anti-C1q) and glomerular basement membrane (anti-GBM) in pLN. METHODS: Serum samples of 42 patients with pediatric Systemic Lupus Erythematosus (pSLE) (22 with pLN and 20 without renal involvement), fifteen patients with other autoimmune nephritis (AN) and 26 healthy controls (HCs) were examined using ELISA...
December 9, 2016: Clinical and Experimental Immunology
https://www.readbyqxmd.com/read/27936346/antibodies-to-staphylococcus-aureus-capsular-polysaccharides-5-and-8-perform-similarly-in-vitro-but-are-functionally-distinct-in-vivo
#3
Bo Liu, Saeyoung Park, Christopher D Thompson, Xue Li, Jean C Lee
The capsular polysaccharide (CP) produced by Staphylococcus aureus is a virulence factor that allows the organism to evade uptake and killing by host neutrophils. Polyclonal antibodies to the serotype 5 (CP5) and type 8 (CP8) capsular polysaccharides are opsonic and protect mice against experimental bacteremia provoked by encapsulated staphylococci. Thus, passive immunotherapy using CP antibodies has been considered for the prevention or treatment of invasive antibiotic-resistant S. aureus infections. In this report, we generated monoclonal antibodies (mAbs) against S...
December 9, 2016: Virulence
https://www.readbyqxmd.com/read/27936344/safety-and-immunogenicity-of-a-herpes-zoster-subunit-vaccine-in-japanese-population-aged-%C3%A2-50%C3%A2-years-when-administered-subcutaneously-versus-intramuscularly
#4
Peter Vink, Masanari Shiramoto, Masayuki Ogawa, Masahiro Eda, Martine Douha, Thomas Heineman, Himal Lal
The impact of alternate routes of vaccine administration, subcutaneous (SC) or intramuscular (IM), on the safety and immunogenicity of herpes zoster subunit candidate vaccine (HZ/su) was assessed in Japanese adults aged ≥ 50 years. During this phase III open-label study, 60 subjects were randomized (1:1) to receive HZ/su through SC or IM routes in a 0, 2 month schedule. Vaccine response rates (VRRs) and geometric mean concentrations (GMCs) of varicella zoster virus glycoprotein E (gE)-specific antibodies were determined by ELISA...
December 9, 2016: Human Vaccines & Immunotherapeutics
https://www.readbyqxmd.com/read/27936222/combined-oral-and-intravenous-immunization-stimulates-strong-iga-responses-in-both-systemic-and-mucosal-compartments
#5
Zhe Yang, Qing Zhao, Yun-An Gao, Wei Zhang
To investigate the influence of immunization routes onIgG, IgA and IgM production in systemic and mucosal compartments, we immunized mice with keyhole limpet hemocyanin (KLH) via oral, intranasal (i.n.) or subcutaneous (s.c.) routes alone or combined with the intravenous (i.v.) route. We found that administering antigen intravenously could affect antibody production and formation of antibody secreting cells (ASCs) depending on the immunization route previously used. Combined oral/i.v. immunization but not s...
2016: PloS One
https://www.readbyqxmd.com/read/27936194/change-in-18f-fluoromisonidazole-pet-is-an-early-predictor-of-the-prognosis-in-the-patients-with-recurrent-high-grade-glioma-receiving-bevacizumab-treatment
#6
Shigeru Yamaguchi, Kenji Hirata, Takuya Toyonaga, Kentaro Kobayashi, Yukitomo Ishi, Hiroaki Motegi, Hiroyuki Kobayashi, Tohru Shiga, Nagara Tamaki, Shunsuke Terasaka, Kiyohiro Houkin
BACKGROUND: Bevacizumab (BEV), a humanized monoclonal antibody, become a currently important chemotherapeutic option for the patients with recurrent glioma. The aim of this retrospective study is to investigate whether 18F-Fluoromisonidazole (FMISO) PET have the potential to detect BEV-resistant gliomas in the early-stage. METHODS: We reviewed the FMISO PET and MRI appearances before and 3 to 4 courses after BEV treatment on 18 recurrent glioma patients. FMISO accumulation was assessed by visual inspection and semi-quantitative values which were tumor-to-normal (T/N) ratio and hypoxic volume...
2016: PloS One
https://www.readbyqxmd.com/read/27936193/occurrence-of-asymptomatic-acute-neuromyelitis-optica-spectrum-disorder-typical-brain-lesions-during-an-attack-of-optic-neuritis-or-myelitis
#7
Su-Hyun Kim, Jae-Won Hyun, AeRan Joung, Sang Hyun Lee, Ho Jin Kim
We aimed to investigate the frequency of asymptomatic acute brain MRI abnormalities accompanying optic neuritis (ON) or myelitis in neuromyelitis optica spectrum disorder (NMOSD) patients with aquaporin-4 antibodies (AQP4-Ab). We reviewed 324 brain MRI scans that were obtained during acute attacks of ON or myelitis, in 165 NMOSD patients with AQP4-Ab. We observed that acute asymptomatic NMOSD-typical brain lesions accompanied 27 (8%) acute attacks of ON or myelitis in 24 (15%) patients. The most common asymptomatic brain abnormalities included edematous corpus callosum lesions (n = 17), followed by lesions on the internal capsule and/or cerebral peduncle lesions (n = 9), periependymal surfaces of the fourth ventricle (n = 5), large deep white matter lesions (n = 4), periependymal cerebral lesions surrounding the lateral ventricles (n = 3), and hypothalamic lesions (n = 1)...
2016: PloS One
https://www.readbyqxmd.com/read/27936131/immunolocalization-of-a-histidine-rich-epidermal-differentiation-protein-in-the-chicken-supports-the-hypothesis-of-an-evolutionary-developmental-link-between-the-embryonic-subperiderm-and-feather-barbs-and-barbules
#8
Lorenzo Alibardi, Karin Brigit Holthaus, Supawadee Sukseree, Marcela Hermann, Erwin Tschachler, Leopold Eckhart
The morphogenesis of feathers is a complex process that depends on a tight spatiotemporal regulation of gene expression and assembly of the protein components of mature feathers. Recent comparative genomics and gene transcription studies have indicated that genes within the epidermal differentiation complex (EDC) encode numerous structural proteins of cornifying skin cells in amniotes including birds. Here, we determined the localization of one of these proteins, termed EDMTFH (Epidermal Differentiation Protein starting with a MTF motif and rich in Histidine), which belongs to a group of EDC-encoded proteins rich in aromatic amino acid residues...
2016: PloS One
https://www.readbyqxmd.com/read/27936119/anti-tnf-%C3%AE-drugs-differently-affect-the-tnf%C3%AE-stnfr-system-and-monocyte-subsets-in-patients-with-psoriasis
#9
Lara Gibellini, Sara De Biasi, Elena Bianchini, Regina Bartolomeo, Antonella Fabiano, Marco Manfredini, Federica Ferrari, Giuseppe Albertini, Tommaso Trenti, Milena Nasi, Marcello Pinti, Anna Iannone, Carlo Salvarani, Andrea Cossarizza, Giovanni Pellacani
TNF-α has a central role in the development and maintenance of psoriatic plaques, and its serum levels correlate with disease activity. Anti-TNF-α drugs are, however, ineffective in a relevant percentage of patients for reasons that are currently unknown. To understand whether the response to anti-TNF-α drugs is influenced by the production of anti-drug antibodies or by the modulation of the TNFα-TNFα receptor system, and to identify changes in monocyte phenotype and activity, we analysed 119 psoriatic patients who either responded or did not respond to different anti-TNF-α therapies (adalimumab, etanercept or infliximab), and measured plasma levels of TNF-α, TNF-α soluble receptors, drug and anti-drug antibodies...
2016: PloS One
https://www.readbyqxmd.com/read/27936076/angiogenic-capacity-of-periodontal-ligament-stem-cells-pretreated-with-deferoxamine-and-or-fibroblast-growth-factor-2
#10
Jessica Ratajczak, Petra Hilkens, Pascal Gervois, Esther Wolfs, Reinhilde Jacobs, Ivo Lambrichts, Annelies Bronckaers
Periodontal ligament stem cells (PDLSCs) represent a good source of multipotent cells for cell-based therapies in regenerative medicine. The success rate of these treatments is severely dependent on the establishment of adequate vasculature in order to provide oxygen and nutrients to the transplanted cells. Pharmacological preconditioning of stem cells has been proposed as a promising method to augment their therapeutic efficacy. In this study, the aim was to improve the intrinsic angiogenic properties of PDLSCs by in vitro pretreatment with deferoxamine (DFX; 100μM), fibroblast growth factor-2 (FGF-2; 10ng/mL) or both substances combined...
2016: PloS One
https://www.readbyqxmd.com/read/27936047/cross-neutralizing-antibodies-in-hiv-1-individuals-infected-by-subtypes-b-f1-c-or-the-b-bbr-variant-in-relation-to-the-genetics-and-biochemical-characteristics-of-the-env-gene
#11
Dalziza Victalina de Almeida, Karine Venegas Macieira, Beatriz Gilda Jegerhorn Grinsztejn, Valdiléa Gonçalves Veloso Dos Santos, Monick Lindenmeyer Guimarães
Various HIV-1 env genetic and biochemical features impact the elicitation of cross-reactive neutralizing antibodies in natural infections. Thus, we aimed to investigate cross-neutralizing antibodies in individuals infected with HIV-1 env subtypes B, F1, C or the B/Bbr variant as well as env characteristics. Therefore, plasma samples from Brazilian chronically HIV-1 infected individuals were submitted to the TZM-bl neutralization assay. We also analyzed putative N-glycosylation sites (PNGLs) and the size of gp120 variable domains in the context of HIV-1 subtypes prevalent in Brazil...
2016: PloS One
https://www.readbyqxmd.com/read/27936034/flow-cytometry-as-a-tool-for-quality-control-of-fluorescent-conjugates-used-in-immunoassays
#12
Marta de Almeida Santiago, Bruna de Paula Fonseca E Fonseca, Christiane de Fátima da Silva Marques, Edimilson Domingos da Silva, Alvaro Luiz Bertho, Ana Cristina Martins de Almeida Nogueira
The use of antibodies in immunodiagnostic kits generally implies the conjugation of these proteins with other molecules such as chromophores or fluorochromes. The development of more sensitive quality control procedures than spectrophotometry is essential to assure the use of better fluorescent conjugates since the fluorescent conjugates are critical reagents for a variety of immunodiagnostic kits. In this article, we demonstrate a new flow cytometric protocol to evaluate conjugates by molecules of equivalent soluble fluorochromes (MESF) and by traditional flow cytometric analysis...
2016: PloS One
https://www.readbyqxmd.com/read/27936017/cellular-prion-protein-combined-with-galectin-3-and-6-affects-the-infectivity-titer-of-an-endogenous-retrovirus-assayed-in-hippocampal-neuronal-cells
#13
Boe-Hyun Kim, Hae-Young Shin, Joy J Goto, Richard I Carp, Eun-Kyoung Choi, Yong-Sun Kim
Prion diseases are infectious and fatal neurodegenerative diseases which require the cellular prion protein, PrPC, for development of diseases. The current study shows that the PrPC augments infectivity and plaque formation of a mouse endogenous retrovirus, MuLV. We have established four neuronal cell lines expressing mouse PrPC, PrP+/+; two express wild type PrPC (MoPrPwild) and the other two express mutant PrPC (MoPrPmut). Infection of neuronal cells from various PrP+/+ and PrP-/- (MoPrPKO) lines with MuLV yielded at least three times as many plaques in PrP+/+ than in PrP-/-...
2016: PloS One
https://www.readbyqxmd.com/read/27936014/inhibition-of-interleukin-6-receptor-in-a-murine-model-of-myocardial-ischemia-reperfusion
#14
Minke H T Hartman, Inge Vreeswijk-Baudoin, Hilde E Groot, Kees W A van de Kolk, Rudolf A de Boer, Irene Mateo Leach, Rozemarijn Vliegenthart, Herman H W Sillje, Pim van der Harst
BACKGROUND: Interleukin-6 (IL-6) levels are upregulated in myocardial infarction. Recent data suggest a causal role of the IL-6 receptor (IL-6R) in coronary heart disease. We evaluated if IL-6R blockade by a monoclonal antibody (MR16-1) prevents the heart from adverse left ventricular remodeling in a mouse model of ischemia-reperfusion (I/R). METHODS: CJ57/BL6 mice underwent I/R injury (left coronary artery ligation for 45 minutes) or sham surgery, and thereafter received MR16-1 (2mg/mouse) 5 minutes before reperfusion and 0...
2016: PloS One
https://www.readbyqxmd.com/read/27935943/confined-mobility-of-tonb-and-fepa-in-escherichia-coli-membranes
#15
Yoriko Lill, Lorne D Jordan, Chuck R Smallwood, Salete M Newton, Markus A Lill, Phillip E Klebba, Ken Ritchie
The important process of nutrient uptake in Escherichia coli, in many cases, involves transit of the nutrient through a class of beta-barrel proteins in the outer membrane known as TonB-dependent transporters (TBDTs) and requires interaction with the inner membrane protein TonB. Here we have imaged the mobility of the ferric enterobactin transporter FepA and TonB by tracking them in the membranes of live E. coli with single-molecule resolution at time-scales ranging from milliseconds to seconds. We employed simple simulations to model/analyze the lateral diffusion in the membranes of E...
2016: PloS One
https://www.readbyqxmd.com/read/27935862/targeting-of-interleukin-il-17a-inhibits-pdl1-expression-in-tumor-cells-and-induces-anticancer-immunity-in-an-estrogen-receptor-negative-murine-model-of-breast-cancer
#16
Yun-Feng Ma, Chen Chen, Dongqing Li, Min Liu, Zhuang-Wei Lv, Yanhong Ji, Jiru Xu
The expression of IL-17A and programmed death ligand 1 (PDL1) is increased in estrogen receptor-negative breast cancer. IL-17A promotes tumor cell survival and invasiveness and inhibits the antitumor immune response. The PDL1-PD1 (programmed death protein 1) signaling pathway promotes escape from immune surveillance in tumor cells. The pro-tumor properties of IL-17A and PDL1 in various cancers have been previously examined; however, the relationship and roles of IL-17A and PDL1 in ER-negative breast cancer have not been evaluated...
December 7, 2016: Oncotarget
https://www.readbyqxmd.com/read/27935674/multiplexed-imaging-of-trace-residues-in-a-single-latent-fingerprint
#17
Yuyan Zhang, Wen Zhou, Yang Xue, Jie Yang, Dingbin Liu
The development of highly sensitive, selective, nondestructive, and multiplexed imaging modalities is essential for latent fingerprint (LFP) identification and fingerprint residues detection. Herein, we present a versatile strategy to identify LFPs and to probe the multiple trace residues in a single LFP simultaneously. With the purpose of achieving high sensitivity, we for the first time introduced a polydopamine (PDA)-triggered Au growth method to prepare superbright and multiplex surface-enhanced Raman scattering (SERS) tags, which were endowed with high selectivity by conjugating with specific antibodies...
December 9, 2016: Analytical Chemistry
https://www.readbyqxmd.com/read/27935326/role-of-cd56-in-normal-kidney-development-and-wilms-tumorigenesis
#18
Li-Wei Yap, Jesper Brok, Kathy Pritchard-Jones
The cell-surface glycoprotein CD56 has three major isoforms that play important roles in cell adhesion and signaling, which may promote cell proliferation, differentiation, survival, or migration. It is an important molecule in normal kidney development and acts as a key marker in Wilms tumor stem and progenitor cells. Here, we review the structural and genetic features of the CD56 glycoprotein, and summarize its roles in the normal versus diseased metanephric blastema. We discuss areas of CD56-related research that may complement or improve existing Wilms tumor treatment strategies, including the antibody-drug conjugate lorvotuzumab mertansine that binds to CD56...
December 9, 2016: Fetal and Pediatric Pathology
https://www.readbyqxmd.com/read/27935290/aromatic-functionality-of-target-proteins-influences-monomer-selection-for-creating-artificial-antibodies-on-plasmonic-biosensors
#19
Rong Hu, Jingyi Luan, Evan D Kharasch, Srikanth Singamaneni, Jeremiah J Morrissey
Natural antibodies used as biorecognition elements suffer from numerous shortcomings, such as limited chemical and environmental stability and cost. Artificial antibodies based on molecular imprinting are an attractive alternative to natural antibodies. We investigated the role of aromatic interactions in target recognition capabilities of artificial antibodies. Three proteins with different aromatic amino acid content were employed as model targets. Artificial antibodies were formed on nanostructures using combinations of silane monomers of varying aromatic functionality...
December 9, 2016: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/27935157/celiac-patients-detected-during-type-1-diabetes-surveillance-had-similar-issues-to-those-diagnosed-on-a-clinical-basis
#20
Anna U Laitinen, Daniel Agardh, Laura Kivelä, Heini Huhtala, Marja-Leena Lähdeaho, Katri Kaukinen, Kalle Kurppa
AIM: Screening children with type 1 diabetes for celiac disease is controversial, because they often appear asymptomatic. Our aim was to establish whether active screening should be recommended. METHODS: This study focused on 22 children whose celiac disease was detected by serological screening during diabetes surveillance and 498 children diagnosed because of a clinical suspicion. We compared the clinical and histological data at diagnosis and the children's adherence and responses to a gluten-free diet...
December 9, 2016: Acta Paediatrica
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