keyword
MENU ▼
Read by QxMD icon Read
search

HDL Proteomics

keyword
https://www.readbyqxmd.com/read/29226278/effects-of-multiple-freeze-thaw-cycles-on-measurements-of-potential-novel-biomarkers-associated-with-adverse-pregnancy-outcomes
#1
Sandra L Rebholz, John T Melchior, Jeffrey A Welge, Alan T Remaley, W Sean Davidson, Laura A Woollett
World-wide, millions of women enter preterm labor or have small newborns. Effective biomarkers are needed to identify women at risk for these adverse outcomes. A time and cost effective way to examine any potentially new biomarkers in samples collected during prior studies or trials that had been assayed for other metabolites would be highly useful. Thus, the current study aimed to determine if samples that had been previously thawed and re-frozen could be re-assayed for novel biomarkers, those being lipoprotein composition (sizing, proteome, lipids) and combined cholesterol and cytokine concentrations...
2017: Journal of Clinical and Laboratory Medicine
https://www.readbyqxmd.com/read/29149817/high-density-lipoprotein-hdl-dysfunction-and-future-of-hdl
#2
Sibel Ertek
Although high density lipoprotein cholesterol (HDL-C) levels are inversely proportional to cardiovascular risk in many studies, recent pharmacological interventional studies with HDL-C raising strategies did not show a benefit in terms of vascular events. The HDL particle is heterogenous with anti-atherogenic functions and non-vascular effects. Many factors affect HDL components and may either cause compositional changes, post-translational modifications of proteins, or alter lipids and other cargo molecules; generally these factors cause more than one of these changes, resulting in functional differences...
November 16, 2017: Current Vascular Pharmacology
https://www.readbyqxmd.com/read/29142235/proteomic-study-revealed-cellular-assembly-and-lipid-metabolism-dysregulation-in-sepsis-secondary-to-community-acquired-pneumonia
#3
Narendra Kumar Sharma, Alexandre Keiji Tashima, Milena Karina Colo Brunialti, Eden Ramalho Ferreira, Ricardo Jose Soares Torquato, Renato Arruda Mortara, Flavia Ribeiro Machado, Murillo Assuncao, Otelo Rigato, Reinaldo Salomao
Sepsis is a life-threatening disorder characterized by organ dysfunction and a major cause of mortality worldwide. The major challenge in studying sepsis is its diversity in such factors as age, source of infection and etiology. Recently, genomic and proteomic approaches have improved our understanding of its complex pathogenesis. In the present study, we use quantitative proteomics to evaluate the host proteome response in septic patients secondary to community-acquired pneumonia (CAP). Samples obtained at admission and after 7 days of follow-up were analyzed according to the outcomes of septic patients...
November 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29115584/apolipoprotein-c-iii-in-the-high-density-lipoprotein-proteome-of-cerebral-lacunar-infarction-patients-impairs-its-anti-inflammatory-function
#4
Pu Lv, Mingming Zhao, Yuanyuan Liu, Haiqiang Jin, Wei Cui, Chenghe Fan, Yuming Teng, Lemin Zheng, Yining Huang
High-density lipoprotein (HDL) proteomic study has identified substantial changes associated with various disease states. In the current study, the HDL proteomes in patients with cerebral lacunar infarction (LACI) and control subjects were investigated. A total of 12 LACI patients without evident large vessel occlusions and 12 controls were enrolled in the study. The HDL fraction from each sample was isolated from the plasma by ultracentrifugation. The protemics of the HDL were investigated using nano liquid chromatography coupled to tandem mass spectrometry...
October 27, 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/29113513/quantifying-hdl-proteins-by-mass-spectrometry-how-many-proteins-are-there-and-what-are-their-functions
#5
Baohai Shao, Jay W Heinecke
Many lines of evidence indicate that low levels of HDL cholesterol increase the risk of cardiovascular disease (CVD). However, recent clinical studies of statin-treated subjects with established atherosclerosis cast doubt on the hypothesis that elevating HDL cholesterol levels reduces CVD risk. Areas covered: It is critical to identify new HDL metrics that capture HDL's proposed cardioprotective effects. One promising approach is quantitative MS/MS-based HDL proteomics. This article focuses on recent studies of the feasibility and challenges of using this strategy in translational studies...
November 8, 2017: Expert Review of Proteomics
https://www.readbyqxmd.com/read/29106519/desmocollin-1-is-abundantly-expressed-in-atherosclerosis-and-impairs-high-density-lipoprotein-biogenesis
#6
Hong Y Choi, Isabelle Ruel, Abba Malina, David R Garrod, Michael N Oda, Jerry Pelletier, Adel Schwertani, Jacques Genest
Aims: The biogenesis of high-density lipoprotein (HDL) particles by cholesterol-laden foam cells in atherosclerotic lesions is crucial for the removal of excess cholesterol from the lesions. Impairment in the HDL biogenic process contributes to the progression of atherosclerosis. The aim of this study is to identify novel cellular factors regulating HDL biogenesis. Methods and results: HDL biogenesis is a process of apolipoprotein (apo)-mediated solubilization of specific plasma membrane (PM) microdomains generated in cholesterol-accumulated cells...
June 29, 2017: European Heart Journal
https://www.readbyqxmd.com/read/29077935/glycation-reduces-the-stability-of-apoai-and-increases-hdl-dysfunction-in-diet-controlled-type-2-diabetes
#7
Sangeeta R Kashyap, Abdullah Osme, Serguei Ilchenko, Makan Golizeh, Kwangwon Lee, Shuhui Wang, James Bena, Stephen F Previs, Jonathan D Smith, Takhar Kasumov
Context: Hyperglycemia plays a key role in the pathogenesis of cardiovascular complications of diabetes. T2DM is associated with high density lipoprotein (HDL) dysfunction and increased degradation of ApoAI. The mechanism(s) of these changes are largely unknown. Objective: To study the role of hyperglycemia-induced glycation on ApoAI kinetics and stability in patients with diet-controlled T2DM. Design: 2H2O-metabolic labeling approach was used to study ApoAI turnover in patients with diet-controlled T2DM (N= 9 (5F), 59...
October 25, 2017: Journal of Clinical Endocrinology and Metabolism
https://www.readbyqxmd.com/read/28936395/antioxidative-activity-of-high-density-lipoprotein-hdl-mechanistic-insights-into-potential-clinical-benefit
#8
REVIEW
Fernando Brites, Maximiliano Martin, Isabelle Guillas, Anatol Kontush
Uptake of low-density lipoprotein (LDL) particles by macrophages represents a key step in the development of atherosclerotic plaques, leading to the foam cell formation. Chemical modification of LDL is however necessary to induce this process. Proatherogenic LDL modifications include aggregation, enzymatic digestion and oxidation. LDL oxidation by one-electron (free radicals) and two-electron oxidants dramatically increases LDL affinity to macrophage scavenger receptors, leading to rapid LDL uptake and fatty streak formation...
December 2017: BBA Clinical
https://www.readbyqxmd.com/read/28883884/itraq-based-quantitative-proteomics-analysis-of-the-protective-effect-of-yinchenwuling-powder-on-hyperlipidemic-rats
#9
Zheyu Zhang, Wenbo Wang, Ling Jin, Xin Cao, Gonghui Jian, Ning Wu, Xia Xu, Ye Yao, Dongsheng Wang
Yinchenwuling powder (YCL) is an effective traditional Chinese medicine formula to modulate lipid levels. In this study, we established hyperlipidemic rat models and treated them with YCL. The serum concentrations of lipid, malondialdehyde (MDA), endothelin-1 (ET-1), and calcitonin gene-related peptide (CGRP) were measured. Adventitia-free vascular proteins between hyperlipidemic rats and YCL-treated rats were identified using iTRAQ-based quantitative proteomics research approach. Proteins with 1.3-fold difference were analyzed through bioinformatics, and proteomic results were verified by Western blot...
2017: Evidence-based Complementary and Alternative Medicine: ECAM
https://www.readbyqxmd.com/read/28768900/plasminogen-promotes-cholesterol-efflux-by-the-abca1-pathway
#10
Nathalie Pamir, Patrick M Hutchins, Graziella E Ronsein, Hao Wei, Chongren Tang, Riku Das, Tomas Vaisar, Edward Plow, Volker Schuster, Catherine A Reardon, Richard Weinberg, David A Dichek, Santica Marcovina, Godfrey S Getz, Jay W Heinecke
Using genetic and biochemical approaches, we investigated proteins that regulate macrophage cholesterol efflux capacity (CEC) and ABCA1-specific CEC (ABCA1 CEC), 2 functional assays that predict cardiovascular disease (CVD). Macrophage CEC and the concentration of HDL particles were markedly reduced in mice deficient in apolipoprotein A-I (APOA1) or apolipoprotein E (APOE) but not apolipoprotein A-IV (APOA4). ABCA1 CEC was markedly reduced in APOA1-deficient mice but was barely affected in mice deficient in APOE or APOA4...
August 3, 2017: JCI Insight
https://www.readbyqxmd.com/read/28754556/kidney-function-is-associated-with-an-altered-protein-composition-of-high-density-lipoprotein
#11
Katya B Rubinow, Clark M Henderson, Cassianne Robinson-Cohen, Jonathan Himmelfarb, Ian H de Boer, Tomas Vaisar, Bryan Kestenbaum, Andrew N Hoofnagle
Patients with chronic kidney disease (CKD) exhibit a myriad of metabolic derangements, including dyslipidemia characterized by low plasma concentrations of high-density lipoprotein (HDL)-associated cholesterol. However, the effects of kidney disease on HDL composition have not been comprehensively determined. Here we used a targeted mass spectrometric approach to quantify 38 proteins contained in the HDL particles within a CKD cohort of 509 participants with a broad range of estimated glomerular filtration rates (eGFRs) (CKD stages I-V, and a mean eGFR of 45...
December 2017: Kidney International
https://www.readbyqxmd.com/read/28683964/detrimental-effect-of-hypercholesterolemia-on-high-density-lipoprotein-particle-remodeling%C3%A2-in%C3%A2-pigs
#12
Teresa Padró, Judit Cubedo, Sandra Camino, Maria Teresa Béjar, Soumaya Ben-Aicha, Guiomar Mendieta, Joan Carles Escolà-Gil, Rafael Escate, Manuel Gutiérrez, Laura Casani, Lina Badimon, Gemma Vilahur
BACKGROUND: Beneficial effects of high-density lipoproteins (HDL) seem altered in patients with symptomatic cardiovascular disease. We recently demonstrated in a swine model of ischemia-reperfusion (IR) that hypercholesterolemia abolishes HDL-related cardioprotection. OBJECTIVES: This study sought to investigate, using the same animal model, whether the reported impairment of HDL cardioprotective function was associated with alterations in HDL remodeling and functionality...
July 11, 2017: Journal of the American College of Cardiology
https://www.readbyqxmd.com/read/28608804/phytosterols-and-omega-3-supplementation-exert-novel-regulatory-effects-on-metabolic-and-inflammatory-pathways-a-proteomic-study
#13
Carmen Lambert, Judit Cubedo, Teresa Padró, Joan Sánchez-Hernández, Rosa M Antonijoan, Antonio Perez, Lina Badimon
Cardiovascular disease (CVD) remains one of the major causes of death and disability worldwide. In addition to drug treatment, nutritional interventions or supplementations are becoming a health strategy for CVD prevention. Phytosterols (PhyS) are natural components that have been shown to reduce cholesterol levels; while poly-unsaturated fatty acids (PUFA), mainly omega-3 (ω3) fatty acids, have shown to reduce triglyceride levels. Here we aimed to investigate whether the proteins in the main lipoproteins (low density lipoproteins (LDL) and high density lipoproteins (HDL)) as well as proteins in the lipid free plasma fraction (LPDP) were regulated by the intake of PhyS-milk or ω3-milk, in overweight healthy volunteers by a proteomic based systems biology approach...
June 13, 2017: Nutrients
https://www.readbyqxmd.com/read/28486149/dietary-supplementation-with-long-chain-monounsaturated-fatty-acid-isomers-decreases-atherosclerosis-and-alters-lipoprotein-proteomes-in-ldlr-mice
#14
Zhi-Hong Yang, Scott M Gordon, Denis Sviridov, Shuibang Wang, Robert L Danner, Milton Pryor, Boris Vaisman, Yuka Shichijo, Nobushige Doisaki, Alan T Remaley
BACKGROUND AND AIMS: Concentrated fish oils, containing a mixture of long-chain monounsaturated fatty acids (LCMUFA) with aliphatic chains longer than 18 C atoms (i.e., C20:1 and C22:1), have been shown to attenuate atherosclerosis development in mouse models. It is not clear, however, how individual LCMUFA isomers may act on atherosclerosis. METHODS: In the present study, we used saury fish oil-derived concentrates enriched in either C20:1 or C22:1 isomer fractions to investigate their individual effect on atherosclerosis and lipoprotein metabolism...
July 2017: Atherosclerosis
https://www.readbyqxmd.com/read/28476857/apolipoprotein-a-ii-alters-the-proteome-of-human-lipoproteins-and-enhances-cholesterol-efflux-from-abca1
#15
John T Melchior, Scott E Street, Allison B Andraski, Jeremy D Furtado, Frank M Sacks, Rebecca L Shute, Emily I Greve, Debi K Swertfeger, Hailong Li, Amy S Shah, L Jason Lu, W Sean Davidson
HDLs are a family of heterogeneous particles that vary in size, composition, and function. The structure of most HDLs is maintained by two scaffold proteins, apoA-I and apoA-II, but up to 95 other "accessory" proteins have been found associated with the particles. Recent evidence suggests that these accessory proteins are distributed across various subspecies and drive specific biological functions. Unfortunately, our understanding of the molecular composition of such subspecies is limited. To begin to address this issue, we separated human plasma and HDL isolated by ultracentrifugation (UC-HDL) into particles with apoA-I and no apoA-II (LpA-I) and those with both apoA-I and apoA-II (LpA-I/A-II)...
July 2017: Journal of Lipid Research
https://www.readbyqxmd.com/read/28410926/effect-of-high-density-lipoprotein-cholesterol-on-the-relationship-of-serum-iron-and-hemoglobin-with-kidney-function-in-diabetes
#16
Ashley N Williams, Baqiyyah N Conway
Findings of increased hemoglobin inside the HDL proteome among persons with diabetes who have haptoglobin 2-2 genotype suggest that iron-induced lipid peroxidation may be involved in diabetic kidney disease. We investigated the relationships of serum hemoglobin and iron with kidney function, and whether this varied by level of HDLc, in 5296 adults with and 49,161 without diabetes. Estimated eGFR was our marker of kidney function. Hemoglobin was positively associated with eGFR among those with diabetes and inversely among those without diabetes (interaction p-value <0...
June 2017: Journal of Diabetes and its Complications
https://www.readbyqxmd.com/read/28223350/mapping-atheroprotective-functions-and-related-proteins-lipoproteins-in-size-fractionated-human-plasma
#17
Debi K Swertfeger, Hailong Li, Sandra Rebholz, Xiaoting Zhu, Amy S Shah, W Sean Davidson, Long J Lu
HDL has been shown to possess a variety of cardio-protective functions, including removal of excess cholesterol from the periphery, and inhibition of lipoprotein oxidation. It has been proposed that various HDL subparticles exist, each with distinct protein and lipid compositions, which may be responsible for HDL's many functions. We hypothesized that HDL functions will co-migrate with the operational lipoprotein subspecies when separated by gel filtration chromatography. Plasma from 10 healthy male donors was fractionated and the protein composition of the phospholipid containing fractions was analyzed by mass spectrometry (MS)...
April 2017: Molecular & Cellular Proteomics: MCP
https://www.readbyqxmd.com/read/28209220/very-low-density-lipoprotein-associated-apolipoproteins-predict-cardiovascular-events-and-are-lowered-by-inhibition%C3%A2-of%C3%A2-apoc-iii
#18
Raimund Pechlaner, Sotirios Tsimikas, Xiaoke Yin, Peter Willeit, Ferheen Baig, Peter Santer, Friedrich Oberhollenzer, Georg Egger, Joseph L Witztum, Veronica J Alexander, Johann Willeit, Stefan Kiechl, Manuel Mayr
BACKGROUND: Routine apolipoprotein (apo) measurements for cardiovascular disease (CVD) are restricted to apoA-I and apoB. Here, the authors measured an unprecedented range of apolipoproteins in a prospective, population-based study and relate their plasma levels to risk of CVD. OBJECTIVES: This study sought to measure apolipoproteins directly by mass spectrometry and compare their associations with incident CVD and to obtain a system-level understanding of the correlations of apolipoproteins with the plasma lipidome and proteome...
February 21, 2017: Journal of the American College of Cardiology
https://www.readbyqxmd.com/read/28002079/unbiased-and-targeted-mass-spectrometry-for-the-hdl-proteome
#19
Sasha A Singh, Masanori Aikawa
PURPOSE OF REVIEW: Mass spectrometry is an ever evolving technology that is equipped with a variety of tools for protein research. Some lipoprotein studies, especially those pertaining to HDL biology, have been exploiting the versatility of mass spectrometry to understand HDL function through its proteome. Despite the role of mass spectrometry in advancing research as a whole, however, the technology remains obscure to those without hands on experience, but still wishing to understand it...
February 2017: Current Opinion in Lipidology
https://www.readbyqxmd.com/read/27989944/top-down-protein-identification-using-isotopic-envelope-fingerprinting
#20
Kaijie Xiao, Fan Yu, Zhixin Tian
For top-down protein database search and identification from tandem mass spectra, our isotopic envelope fingerprinting search algorithm and ProteinGoggle search engine have demonstrated their strength of efficiently resolving heavily overlapping data as well separating non-ideal data with non-ideal isotopic envelopes from ideal ones with ideal isotopic envelopes. Here we report our updated ProteinGoggle 2.0 for intact protein database search with full-capacity. The indispensable updates include users' optional definition of dynamic post-translational modifications and static chemical labeling during database creation, comprehensive dissociation methods and ion series, as well as a Proteoform Score for each proteoform...
January 30, 2017: Journal of Proteomics
keyword
keyword
27765
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"