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HDL Proteomics

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https://www.readbyqxmd.com/read/29649363/a-targeted-differential-top-down-proteomic-methodology-for-comparison-of-apoa-i-proteoforms-in-individuals-with-high-and-low-hdl-efflux-capacity
#1
Henrique Dos Santos Seckler, Luca Fornelli, R Kannan Mutharasan, C Shad Thaxton, Ryan T Fellers, Martha Daviglus, Allan Sniderman, Daniel Rader, Neil L Kelleher, Donald M Lloyd-Jones, Philip D Compton, John T Wilkins
Top-down proteomics (TDP) allows precise determination/characterization of the different proteoforms derived from the expression of a single gene. In this study, we targeted apolipoprotein A-I (ApoA-I), a mediator of high-density-lipoprotein cholesterol efflux (HDL-E), which is inversely associated with coronary heart disease risk. Absolute ApoA-I concentration and allelic variation only partially explain inter-individual HDL-E variation. Therefore, we hypothesize that differences in HDL-E are associated with the abundances of different ApoA-I proteoforms...
April 12, 2018: Journal of Proteome Research
https://www.readbyqxmd.com/read/29623821/long-term-effect-of-lysine-restriction-on-liver-global-proteins-meat-quality-and-blood-biochemical-parameters-in-pigs
#2
Jie Yin, Tiejun Li, Jianhua He
BACKGROUND: Our previous study suggested that short-term lysine restriction improved feed intake and gut microbiota in piglets. Thus, in this study, we further used proteomics technology to investigate the potential mechanism associated with long-term lysine restriction in pigs. In addition, blood biochemical parameters, meat quality, and muscle nutrient transporters were also investigated. RESULTS: The results showed that 131 proteins in the liver were markedly altered in response to dietary lysine restriction, with 54 being downregulated and 77 being upregulated, which mainly involved in neurodegenerative diseases, oxidative phosphorylation, and metabolic pathways...
April 6, 2018: Protein and Peptide Letters
https://www.readbyqxmd.com/read/29561322/hdls-and-the-pathogenesis-of-atherosclerosis
#3
Adel Schwertani, Hong Y Choi, Jacques Genest
PURPOSE OF REVIEW: Plasma levels of HDL cholesterol are a biomarker of cardiovascular health but not a therapeutic target, as demonstrated by the failure of pharmacological modulation of HDL cholesterol to prevent or treat atherosclerotic cardiovascular disease. In health, HDL particles exert pleiotropic effects against atherosclerosis, including cholesterol removal from foam cells, vasodilatory effects through vascular endothelial cell nitric oxide production, decreased vascular inflammation and oxidative damage, endothelial cell proliferation and antiapoptotic effects...
March 19, 2018: Current Opinion in Cardiology
https://www.readbyqxmd.com/read/29527140/proteomic-characterization-of-high-density-lipoprotein-particles-in-patients-with-non-alcoholic-fatty-liver-disease
#4
Prahlad K Rao, Kate Merath, Eugene Drigalenko, Avinash Y L Jadhav, Richard A Komorowski, Matthew I Goldblatt, Anand Rohatgi, Mark A Sarzynski, Samer Gawrieh, Michael Olivier
Background: Metabolic diseases such as obesity and diabetes are associated with changes in high-density lipoprotein (HDL) particles, including changes in particle size and protein composition, often resulting in abnormal function. Recent studies suggested that patients with non-alcoholic fatty liver disease (NAFLD), including individuals with non-alcoholic steatohepatitis (NASH), have smaller HDL particles when compared to individuals without liver pathologies. However, no studies have investigated potential changes in HDL particle protein composition in patients with NAFLD, in addition to changes related to obesity, to explore putative functional changes of HDL which may increase the risk of cardiovascular complications...
2018: Clinical Proteomics
https://www.readbyqxmd.com/read/29505607/myeloperoxidase-mediated-hdl-oxidation-and-hdl-proteome-changes-do-not-contribute-to-dysfunctional-hdl-in-chinese-subjects-with-coronary-artery-disease
#5
Guisong Wang, Anna Vachaparampil Mathew, Haiyi Yu, Lei Li, Liyun He, Wei Gao, Xiaodan Liu, Yanhong Guo, Jaeman Byun, Jifeng Zhang, Y Eugene Chen, Subramaniam Pennathur
High density lipoprotein (HDL) cholesterol levels and cholesterol efflux capacity (CEC) are inversely correlated with coronary artery disease (CAD) risk. Myeloperoxidase (MPO) derived oxidants and HDL proteome changes are implicated in HDL dysfunction in subjects with CAD in the United States; however, the effect of MPO on HDL function and HDL proteome in ethnic Chinese population is unknown. We recruited four matched ethnic Chinese groups (20 patients each): subjects with 1) low HDL levels (HDL levels in men <40mg/dL and women <50mg/dL) and non-CAD (identified by coronary angiography or cardiac CT angiography); 2) low HDL and CAD; 3) high HDL (men >50mg/dL; women >60mg/dL) with no CAD; and 4) high HDL with CAD...
2018: PloS One
https://www.readbyqxmd.com/read/29411613/rapid-affinity-enrichment-of-human-apolipoprotein-a-i-associated-lipoproteins-for-proteome-analysis
#6
Timothy S Collier, Zhicheng Jin, Celalettin Topbas, Cory Bystrom
Isolation of High Density Lipoproteins (HDL) for structural and functional studies typically relies on ultracentrifugation techniques which are time consuming and difficult to scale. With emerging interest in the clinical relevance of HDL structure and function to cardiovascular disease, a significant gap exists between current and desirable sample preparation throughput. To enable proteomic studies of HDL with large clinical cohorts, we have developed an affinity enrichment approach which relies on the association of histidine-tagged, lipid free ApoA-I with HDL followed by standard metal chelate chromatography...
February 7, 2018: Journal of Proteome Research
https://www.readbyqxmd.com/read/29366287/lipidomic-and-proteomic-predictors-of-drastic-reductions-of-hdl-in-the-accord-lipid-trial
#7
Gissette Reyes-Soffer, Maryam Khavani, Stephen Holleran, Rajasekhar Ramakrishnan, Amy Lovato, Henry Ginsberg
No abstract text is available yet for this article.
August 2017: Atherosclerosis
https://www.readbyqxmd.com/read/29366071/shotgun-proteomics-analysis-characterizes-hdl-proteome-in-patients-with-cholesteryl-ester-transfer-protein-deficiency
#8
Takeshi Okada, Tohru Ohama, Kotaro Kanno, Hibiki Matsuda, Masami Sairyo, Yinghong Zhu, Ayami Saga, Takuya Kobayashi, Daisaku Masuda, Masahiro Koseki, Makoto Nishida, Yasushi Sakata, Shizuya Yamashita
No abstract text is available yet for this article.
August 2017: Atherosclerosis
https://www.readbyqxmd.com/read/29251807/proteomic-diversity-of-high-density-lipoprotein-explains-its-association-with-clinical-outcome-in-patients-with-heart-failure
#9
Johanna Elisabeth Emmens, Donald J L Jones, Thong H Cao, Daniel C S Chan, Simon P R Romaine, Paulene A Quinn, Stefan D Anker, John G Cleland, Kenneth Dickstein, Gerasimos Filippatos, Hans L Hillege, Chim C Lang, Piotr Ponikowski, Nilesh J Samani, Dirk J van Veldhuisen, Faiz Zannad, Aeilko H Zwinderman, Marco Metra, Rudolf A de Boer, Adriaan A Voors, Leong L Ng
AIMS: Previously, low high-density lipoprotein (HDL) cholesterol was found to be one of the strongest predictors of mortality and/or heart failure (HF) hospitalisation in patients with HF. We therefore performed in-depth investigation of the multifunctional HDL proteome to reveal underlying pathophysiological mechanisms explaining the association between HDL and clinical outcome. METHODS AND RESULTS: We selected a cohort of 90 HF patients with 1:1 cardiovascular death/survivor ratio from BIOSTAT-CHF...
February 2018: European Journal of Heart Failure
https://www.readbyqxmd.com/read/29226278/effects-of-multiple-freeze-thaw-cycles-on-measurements-of-potential-novel-biomarkers-associated-with-adverse-pregnancy-outcomes
#10
Sandra L Rebholz, John T Melchior, Jeffrey A Welge, Alan T Remaley, W Sean Davidson, Laura A Woollett
World-wide, millions of women enter preterm labor or have small newborns. Effective biomarkers are needed to identify women at risk for these adverse outcomes. A time and cost effective way to examine any potentially new biomarkers in samples collected during prior studies or trials that had been assayed for other metabolites would be highly useful. Thus, the current study aimed to determine if samples that had been previously thawed and re-frozen could be re-assayed for novel biomarkers, those being lipoprotein composition (sizing, proteome, lipids) and combined cholesterol and cytokine concentrations...
2017: Journal of Clinical and Laboratory Medicine
https://www.readbyqxmd.com/read/29149817/high-density-lipoprotein-hdl-dysfunction-and-future-of-hdl
#11
Sibel Ertek
Although high density lipoprotein cholesterol (HDL-C) levels are inversely proportional to cardiovascular risk in many studies, recent pharmacological interventional studies with HDL-C raising strategies did not show a benefit in terms of vascular events. The HDL particle is heterogenous with anti-atherogenic functions and non-vascular effects. Many factors affect HDL components and may either cause compositional changes, post-translational modifications of proteins, or alter lipids and other cargo molecules; generally these factors cause more than one of these changes, resulting in functional differences...
November 16, 2017: Current Vascular Pharmacology
https://www.readbyqxmd.com/read/29142235/proteomic-study-revealed-cellular-assembly-and-lipid-metabolism-dysregulation-in-sepsis-secondary-to-community-acquired-pneumonia
#12
Narendra Kumar Sharma, Alexandre Keiji Tashima, Milena Karina Colo Brunialti, Eden Ramalho Ferreira, Ricardo Jose Soares Torquato, Renato Arruda Mortara, Flavia Ribeiro Machado, Murillo Assuncao, Otelo Rigato, Reinaldo Salomao
Sepsis is a life-threatening disorder characterized by organ dysfunction and a major cause of mortality worldwide. The major challenge in studying sepsis is its diversity in such factors as age, source of infection and etiology. Recently, genomic and proteomic approaches have improved our understanding of its complex pathogenesis. In the present study, we use quantitative proteomics to evaluate the host proteome response in septic patients secondary to community-acquired pneumonia (CAP). Samples obtained at admission and after 7 days of follow-up were analyzed according to the outcomes of septic patients...
November 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29115584/apolipoprotein-c-iii-in-the-high-density-lipoprotein-proteome-of-cerebral-lacunar-infarction-patients-impairs-its-anti-inflammatory-function
#13
Pu Lv, Mingming Zhao, Yuanyuan Liu, Haiqiang Jin, Wei Cui, Chenghe Fan, Yuming Teng, Lemin Zheng, Yining Huang
High-density lipoprotein (HDL) proteomic study has identified substantial changes associated with various disease states. In the current study, the HDL proteomes in patients with cerebral lacunar infarction (LACI) and control subjects were investigated. A total of 12 LACI patients without evident large vessel occlusions and 12 controls were enrolled in the study. The HDL fraction from each sample was isolated from the plasma by ultracentrifugation. The protemics of the HDL were investigated using nano liquid chromatography coupled to tandem mass spectrometry...
January 2018: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/29113513/quantifying-hdl-proteins-by-mass-spectrometry-how-many-proteins-are-there-and-what-are-their-functions
#14
Baohai Shao, Jay W Heinecke
Many lines of evidence indicate that low levels of HDL cholesterol increase the risk of cardiovascular disease (CVD). However, recent clinical studies of statin-treated subjects with established atherosclerosis cast doubt on the hypothesis that elevating HDL cholesterol levels reduces CVD risk. Areas covered: It is critical to identify new HDL metrics that capture HDL's proposed cardioprotective effects. One promising approach is quantitative MS/MS-based HDL proteomics. This article focuses on recent studies of the feasibility and challenges of using this strategy in translational studies...
January 2018: Expert Review of Proteomics
https://www.readbyqxmd.com/read/29106519/desmocollin-1-is-abundantly-expressed-in-atherosclerosis-and-impairs-high-density-lipoprotein-biogenesis
#15
Hong Y Choi, Isabelle Ruel, Abba Malina, David R Garrod, Michael N Oda, Jerry Pelletier, Adel Schwertani, Jacques Genest
Aims: The biogenesis of high-density lipoprotein (HDL) particles by cholesterol-laden foam cells in atherosclerotic lesions is crucial for the removal of excess cholesterol from the lesions. Impairment in the HDL biogenic process contributes to the progression of atherosclerosis. The aim of this study is to identify novel cellular factors regulating HDL biogenesis. Methods and results: HDL biogenesis is a process of apolipoprotein (apo)-mediated solubilization of specific plasma membrane (PM) microdomains generated in cholesterol-accumulated cells...
June 29, 2017: European Heart Journal
https://www.readbyqxmd.com/read/29077935/glycation-reduces-the-stability-of-apoai-and-increases-hdl-dysfunction-in-diet-controlled-type-2-diabetes
#16
Sangeeta R Kashyap, Abdullah Osme, Serguei Ilchenko, Makan Golizeh, Kwangwon Lee, Shuhui Wang, James Bena, Stephen F Previs, Jonathan D Smith, Takhar Kasumov
Context: Hyperglycemia plays a key role in the pathogenesis of cardiovascular complications of diabetes. Type 2 diabetes mellitus (T2DM) is associated with high-density lipoprotein (HDL) dysfunction and increased degradation of apolipoprotein I (ApoAI). The mechanism(s) of these changes is largely unknown. Objective: To study the role of hyperglycemia-induced glycation on ApoAI kinetics and stability in patients with diet-controlled T2DM. Design: 2H2O-metabolic labeling approach was used to study ApoAI turnover in patients with diet-controlled T2DM [n = 9 (5 F); 59...
February 1, 2018: Journal of Clinical Endocrinology and Metabolism
https://www.readbyqxmd.com/read/28936395/antioxidative-activity-of-high-density-lipoprotein-hdl-mechanistic-insights-into-potential-clinical-benefit
#17
REVIEW
Fernando Brites, Maximiliano Martin, Isabelle Guillas, Anatol Kontush
Uptake of low-density lipoprotein (LDL) particles by macrophages represents a key step in the development of atherosclerotic plaques, leading to the foam cell formation. Chemical modification of LDL is however necessary to induce this process. Proatherogenic LDL modifications include aggregation, enzymatic digestion and oxidation. LDL oxidation by one-electron (free radicals) and two-electron oxidants dramatically increases LDL affinity to macrophage scavenger receptors, leading to rapid LDL uptake and fatty streak formation...
December 2017: BBA Clinical
https://www.readbyqxmd.com/read/28883884/itraq-based-quantitative-proteomics-analysis-of-the-protective-effect-of-yinchenwuling-powder-on-hyperlipidemic-rats
#18
Zheyu Zhang, Wenbo Wang, Ling Jin, Xin Cao, Gonghui Jian, Ning Wu, Xia Xu, Ye Yao, Dongsheng Wang
Yinchenwuling powder (YCL) is an effective traditional Chinese medicine formula to modulate lipid levels. In this study, we established hyperlipidemic rat models and treated them with YCL. The serum concentrations of lipid, malondialdehyde (MDA), endothelin-1 (ET-1), and calcitonin gene-related peptide (CGRP) were measured. Adventitia-free vascular proteins between hyperlipidemic rats and YCL-treated rats were identified using iTRAQ-based quantitative proteomics research approach. Proteins with 1.3-fold difference were analyzed through bioinformatics, and proteomic results were verified by Western blot...
2017: Evidence-based Complementary and Alternative Medicine: ECAM
https://www.readbyqxmd.com/read/28768900/plasminogen-promotes-cholesterol-efflux-by-the-abca1-pathway
#19
Nathalie Pamir, Patrick M Hutchins, Graziella E Ronsein, Hao Wei, Chongren Tang, Riku Das, Tomas Vaisar, Edward Plow, Volker Schuster, Catherine A Reardon, Richard Weinberg, David A Dichek, Santica Marcovina, Godfrey S Getz, Jay W Heinecke
Using genetic and biochemical approaches, we investigated proteins that regulate macrophage cholesterol efflux capacity (CEC) and ABCA1-specific CEC (ABCA1 CEC), 2 functional assays that predict cardiovascular disease (CVD). Macrophage CEC and the concentration of HDL particles were markedly reduced in mice deficient in apolipoprotein A-I (APOA1) or apolipoprotein E (APOE) but not apolipoprotein A-IV (APOA4). ABCA1 CEC was markedly reduced in APOA1-deficient mice but was barely affected in mice deficient in APOE or APOA4...
August 3, 2017: JCI Insight
https://www.readbyqxmd.com/read/28754556/kidney-function-is-associated-with-an-altered-protein-composition-of-high-density-lipoprotein
#20
Katya B Rubinow, Clark M Henderson, Cassianne Robinson-Cohen, Jonathan Himmelfarb, Ian H de Boer, Tomas Vaisar, Bryan Kestenbaum, Andrew N Hoofnagle
Patients with chronic kidney disease (CKD) exhibit a myriad of metabolic derangements, including dyslipidemia characterized by low plasma concentrations of high-density lipoprotein (HDL)-associated cholesterol. However, the effects of kidney disease on HDL composition have not been comprehensively determined. Here we used a targeted mass spectrometric approach to quantify 38 proteins contained in the HDL particles within a CKD cohort of 509 participants with a broad range of estimated glomerular filtration rates (eGFRs) (CKD stages I-V, and a mean eGFR of 45...
December 2017: Kidney International
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