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https://www.readbyqxmd.com/read/29136865/a-catalytic-spectrophotometric-method-for-determination-of-nanomolar-manganese-in-seawater-using-reverse-flow-injection-analysis-and-a-long-path-length-liquid-waveguide-capillary-cell
#1
Sichao Feng, Dongxing Yuan, Yongming Huang, Kunning Lin, Yong Zhu, Jian Ma
A sensitive and precise method for determination of nanomolar manganese in seawater was developed, using reverse flow injection analysis, a long path length liquid waveguide capillary cell, and spectrophotometric detection. The reaction was based on manganese catalyzed oxidation of leucomalachite green with sodium periodate. Various experimental parameters were investigated and optimized. Foreign trace metal ions of iron, copper, zinc, nickel and aluminum did not cause obvious interference with manganese detection...
February 1, 2018: Talanta
https://www.readbyqxmd.com/read/29114274/outcomes-of-minimally-invasive-surgery-compared-to-open-posterior-lumbar-instrumentation-and-fusion
#2
Low Yong Lee, Zamzuri Idris, Tan Boon Beng, Teo Yian Young, Wong Chung Chek, Jafri Malin Abdullah, Wong Sii Hieng
Introduction: Degenerative spine disease is increasingly common. There are many spinal fusion techniques used to treat degenerative spine disease. This study aims to compare the functional outcome of open versus minimally invasive surgery (MIS) technique in posterior lumbar instrumentation and fusion in degenerative spine disease and to evaluate the perioperative outcome and complications between MIS and open surgery. Materials and Methods: This is an observational cross-sectional study conducted on all degenerative spine disease patients who underwent both methods of posterior lumbar instrumentation and fusion from 2010 to 2014 by the Orthopedic and Neurosurgery Department, Sarawak General Hospital...
October 2017: Asian Journal of Neurosurgery
https://www.readbyqxmd.com/read/29079797/multi-wavelength-anomalous-diffraction-de-novo-phasing-using-a-two-colour-x-ray-free-electron-laser-with-wide-tunability
#3
Alexander Gorel, Koji Motomura, Hironobu Fukuzawa, R Bruce Doak, Marie Luise Grünbein, Mario Hilpert, Ichiro Inoue, Marco Kloos, Gabriela Kovácsová, Eriko Nango, Karol Nass, Christopher M Roome, Robert L Shoeman, Rie Tanaka, Kensuke Tono, Yasumasa Joti, Makina Yabashi, So Iwata, Lutz Foucar, Kiyoshi Ueda, Thomas R M Barends, Ilme Schlichting
Serial femtosecond crystallography at X-ray free-electron lasers (XFELs) offers unprecedented possibilities for macromolecular structure determination of systems prone to radiation damage. However, de novo structure determination, i.e., without prior structural knowledge, is complicated by the inherent inaccuracy of serial femtosecond crystallography data. By its very nature, serial femtosecond crystallography data collection entails shot-to-shot fluctuations in X-ray wavelength and intensity as well as variations in crystal size and quality that must be averaged out...
October 27, 2017: Nature Communications
https://www.readbyqxmd.com/read/29045166/driver-injury-in-near-and-far-side-impacts-update-on-the-effect-of-front-passenger-belt-use
#4
Chantal S Parenteau, David C Viano
PURPOSE: This is a study that updates earlier research on the influence of a front passenger on the risk for severe driver injury in near-side and far-side impacts. It includes the effects of belt use by the driver and passenger, identifies body regions involved in driver injury and identifies the sources for severe, driver head injury. METHODS: 1997-2015 NASS-CDS data was used to investigate the risk for MAIS 4+F driver injury in near-side and far-side impacts by front-passenger belt use and as a sole occupant in the driver seat...
October 18, 2017: Traffic Injury Prevention
https://www.readbyqxmd.com/read/28983299/deletion-of-the-l-lactate-dehydrogenase-gene-ldh-in-streptococcus-pyogenes-leads-to-a-loss-of-speb-activity-and-a-hypovirulent-phenotype
#5
Sonja Oehmcke-Hecht, Leif E Nass, Jan B Wichura, Stefan Mikkat, Bernd Kreikemeyer, Tomas Fiedler
Streptococcus pyogenes uses lactic acid fermentation for the generation of ATP. Here, we analyzed the impact of a deletion of the L-lactate dehydrogenase gene ldh on the virulence of S. pyogenes M49. While the ldh deletion does not cause a general growth deficiency in laboratory media, the growth in human blood and plasma is significantly hampered. The ldh deletion strain is furthermore less virulent in a Galleria mellonella infection model. We show that the ldh deletion leads to a decrease in the activity of the cysteine protease SpeB, an important secreted virulence factor of S...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28970074/effect-of-patients-functional-status-on-satisfaction-with-outcomes-12-months-after-elective-spine-surgery-for-lumbar-degenerative-disease
#6
Silky Chotai, Clinton J Devin, Kristin R Archer, Mohamad Bydon, Matthew J McGirt, Hui Nian, Frank E Harrell, Robert S Dittus, Anthony L Asher
BACKGROUND: Comprehensive assessment of quality of care includes patient-reported outcomes, safety of care delivered, and patient satisfaction. The impact of the patient-reported ODI (baseline and 12-month) scores on satisfaction with outcomes following spine surgery is not well documented. PURPOSE: To determine the impact of patient disability [Oswestry disability index (ODI) scores] at baseline and 12-months on satisfaction with outcomes following surgery. STUDY DESIGN: Analysis of prospectively collected longitudinal web-based multicenter data...
September 29, 2017: Spine Journal: Official Journal of the North American Spine Society
https://www.readbyqxmd.com/read/28944276/verification-of-neonatal-tetanus-surveillance-systems-in-katsina-state-nigeria
#7
Shafique Sani Nass, Hadi Danawi, Loretta Cain, Monoj Sharma
BACKGROUND: The incidence and mortality rates of neonatal tetanus (NNT) remain underreported in Nigeria. The goal of the study was to compare the NNT prevalence and the mortality rates from the existing surveillance system and active surveillance of health facility records in 7 selected health facilities from 2010 to 2014 in Katsina State, Nigeria. METHODS: The study is a retrospective record review using extracted data from NNT records and analyzed using descriptive statistics...
January 2017: Health Services Research and Managerial Epidemiology
https://www.readbyqxmd.com/read/28942668/recent-exposure-to-hypoglycemia-increases-glucose-variability-following-a-hyper-hypoglycemic-metabolic-challenge-in-t1d
#8
Chiara Fabris, Leon S Farhy, Stacey M Anderson, Ralf M Nass, Boris P Kovatchev, Marc D Breton
AIMS: In type 1 diabetes (T1D), repeated hypoglycemic episodes may reduce hormonal defenses and increase the risk for severe hypoglycemia. In this work, we investigate the effect of a structured hyper/hypoglycemic metabolic challenge on the postintervention glucose variability in T1D subjects studied at home. METHODS: Thirty T1D subjects using insulin pump were monitored with blood glucose meters (SMBG) during a 1-month observation period. After 2 weeks of monitoring, participants were admitted at the University of Virginia Clinical Research Unit to undergo an 8-hour metabolic challenge...
September 1, 2017: Journal of Diabetes Science and Technology
https://www.readbyqxmd.com/read/28927394/laboratory-markers-of-cardiac-and-metabolic-complications-after-generalized-tonic-clonic-seizures
#9
Robert D Nass, Sina Meiling, René P Andrié, Christian E Elger, Rainer Surges
BACKGROUND: Generalized tonic-clonic seizures (GTCS) frequently lead to emergency inpatient referrals. Laboratory blood values are routinely performed on admission to detect underlying causes and metabolic or cardiac complications. Our goal was to assess the nature and frequency of complications occurring in association with GTCS. METHODS: We retrospectively extracted data from emergency protocols and discharge letters of adult patients admitted to the Department of Epileptology between 01/2010 and 06/2015...
September 19, 2017: BMC Neurology
https://www.readbyqxmd.com/read/28916332/assessing-the-use-of-assisted-reproductive-technology-in-the-united-states-by-non-united-states-residents
#10
Aaron D Levine, Sheree L Boulet, Roberta M Berry, Denise J Jamieson, Hillary B Alberta-Sherer, Dmitry M Kissin
OBJECTIVE: To study cross-border reproductive care (CBRC) by assessing the frequency and nature of assisted reproductive technology (ART) care that non-U.S. residents receive in the United States. DESIGN: Retrospective study of ART cycles reported to the Centers for Disease Control and Prevention's National ART Surveillance System (NASS) from 2006 to 2013. SETTING: Private and academic ART clinics. PATIENT(S): Patients who participated in ART cycles in the United States from 2006 to 2013...
November 2017: Fertility and Sterility
https://www.readbyqxmd.com/read/28894435/neuroactive-steroids-receptor-interactions-and-responses
#11
REVIEW
Kald Beshir Tuem, Tesfay Mehari Atey
Neuroactive steroids (NASs) are naturally occurring steroids, which are synthesized centrally as de novo from cholesterol and are classified as pregnane, androstane, and sulfated neurosteroids (NSs). NASs modulate many processes via interacting with gamma-aminobutyric acid (GABA), N-methyl-d-aspartate, serotonin, voltage-gated calcium channels, voltage-dependent anion channels, α-adrenoreceptors, X-receptors of the liver, transient receptor potential channels, microtubule-associated protein 2, neurotrophin nerve growth factor, and σ1 receptors...
2017: Frontiers in Neurology
https://www.readbyqxmd.com/read/28888877/the-role-of-charge-in-the-toxicity-of-polymer-coated-cerium-oxide-nanomaterials-to-caenorhabditis-elegans
#12
Devrah A Arndt, Emily K Oostveen, Judy Triplett, D Allan Butterfield, Olga V Tsyusko, Blanche Collin, Daniel L Starnes, Jian Cai, Jon B Klein, Richard Nass, Jason M Unrine
This study examined the impact of surface functionalization and charge on ceria nanomaterial toxicity to Caenorhabditis elegans. The examined endpoints included mortality, reproduction, protein expression, and protein oxidation profiles. Caenorhabditis elegans were exposed to identical 2-5nm ceria nanomaterial cores which were coated with cationic (diethylaminoethyl dextran; DEAE), anionic (carboxymethyl dextran; CM), and non-ionic (dextran; DEX) polymers. Mortality and reproductive toxicity of DEAE-CeO2 was approximately two orders of magnitude higher than for CM-CeO2 or DEX-CeO2...
October 2017: Comparative Biochemistry and Physiology. Toxicology & Pharmacology: CBP
https://www.readbyqxmd.com/read/28857069/the-endogenous-cannabinoid-system-a-budding-source-of-targets-for-treating-inflammatory-and-neuropathic-pain
#13
Giulia Donvito, Sara R Nass, Jenny L Wilkerson, Zachary A Curry, Lesley D Schurman, Steven G Kinsey, Aron H Lichtman
A great need exists for the development of new medications to treat pain resulting from various disease states and types of injury. Given that the endogenous cannabinoid (ie, endocannabinoid) system modulates neuronal and immune cell function, both of which play key roles in pain, therapeutics targeting this system hold promise as novel analgesics. Potential therapeutic targets include the cannabinoid receptors, type 1 and 2, as well as biosynthetic and catabolic enzymes of the endocannabinoids N-arachidonoylethanolamine and 2-arachidonoylglycerol...
August 31, 2017: Neuropsychopharmacology: Official Publication of the American College of Neuropsychopharmacology
https://www.readbyqxmd.com/read/28843069/new-onset-status-epilepticus-in-older-patients-clinical-characteristics-and-outcome
#14
M P Malter, R D Nass, T Kaluschke, G R Fink, L Burghaus, C Dohmen
PURPOSE: We here evaluated (1) the differential characteristics of status epilepticus (SE) in older (≥60 years) compared to younger adults (18-59 years). In particular, we were interested in (2) the proportion and characteristics of new onset SE in patients with no history of epilepsy (NOSE) in older compared to younger adults, and (3) predictive parameters for clinical outcome in older subjects with NOSE. METHODS: We performed a monocentric retrospective analysis of all adult patients (≥18years) admitted with SE to our tertiary care centre over a period of 10 years (2006-2015) to evaluate clinical characteristics and short-time outcome at discharge...
October 2017: Seizure: the Journal of the British Epilepsy Association
https://www.readbyqxmd.com/read/28840547/cognitive-control-in-media-multitaskers-two-replication-studies-and-a-meta-analysis
#15
Wisnu Wiradhany, Mark R Nieuwenstein
Ophir, Nass, and Wagner (2009, Proceedings of the National Academy of Sciences of the United States of America, 106(37), 15583-15587) found that people with high scores on the media-use questionnaire-a questionnaire that measures the proportion of media-usage time during which one uses more than one medium at the same time-show impaired performance on various tests of distractor filtering. Subsequent studies, however, did not all show this association between media multitasking and distractibility, thus casting doubt on the reliability of the initial findings...
November 2017: Attention, Perception & Psychophysics
https://www.readbyqxmd.com/read/28777970/identification-of-characteristics-and-frequent-scenarios-of-single-vehicle-rollover-crashes-during-pre-ballistic-phase-part-1-a-descriptive-study
#16
Taewung Kim, Dipan Bose, Jon Foster, Varun Bollapragada, Jeff R Crandall, Mark Clauser, Jason R Kerrigan
This study aimed to identify common patterns of pre-ballistic vehicle kinematics and roadway characteristics of real-world rollover crashes. Rollover crashes that were enrolled in the National Automotive Sampling System-Crashworthiness Data System (NASS-CDS) between the years 2000 and 2010 were analyzed. A descriptive analysis was performed to understand the characteristics of the pre-ballistic phase. Also, a frequency based pattern analysis was performed using a selection of NASS-CDS variables describing the pre-ballistic vehicle kinematics and roadway characteristics to rank common pathways of rollover crashes...
August 1, 2017: Accident; Analysis and Prevention
https://www.readbyqxmd.com/read/28767625/patient-reported-outcomes-and-costs-associated-with-revision-surgery-for-degenerative-cervical-spine-diseases
#17
Elliott J Kim, Silky Chotai, Joseph B Wick, David P Stonko, Ahilan Sivaganesan, Clinton J Devin
STUDY DESIGN: Retrospective review of a prospective database. OBJECTIVE: Determine cost and outcomes of revision cervical spine surgery. SUMMARY OF BACKGROUND DATA: Revision rates for cervical spine surgery are steadily increasing. It is important to counsel patients on expected results following a revision procedure. However, outcomes and cost of these procedures are poorly defined in the literature. METHODS: Patients undergoing revision cervical spine surgery at a single institution were included between October 2010 and January 2016 in a prospective registry database...
August 1, 2017: Spine
https://www.readbyqxmd.com/read/28753313/neuroactive-steroids-2-3%C3%AE-hydroxy-3%C3%AE-methyl-21-4-cyano-1h-pyrazol-1-yl-19-nor-5%C3%AE-pregnan-20-one-sage-217-a-clinical-next-generation-neuroactive-steroid-positive-allosteric-modulator-of-the-%C3%AE-aminobutyric-acid-a-receptor
#18
Gabriel Martinez Botella, Francesco G Salituro, Boyd L Harrison, Richard T Beresis, Zhu Bai, Maria-Jesus Blanco, Gabriel M Belfort, Jing Dai, Carlos M Loya, Michael A Ackley, Alison L Althaus, Scott J Grossman, Ethan Hoffmann, James J Doherty, Albert J Robichaud
Certain classes of neuroactive steroids (NASs) are positive allosteric modulators (PAM) of synaptic and extrasynaptic GABAA receptors. Herein, we report new SAR insights in a series of 5β-nor-19-pregnan-20-one analogues bearing substituted pyrazoles and triazoles at C-21, culminating in the discovery of 3α-hydroxy-3β-methyl-21-(4-cyano-1H-pyrazol-1'-yl)-19-nor-5β-pregnan-20-one (SAGE-217, 3), a potent GABAA receptor modulator at both synaptic and extrasynaptic receptor subtypes, with excellent oral DMPK properties...
September 28, 2017: Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/28752517/nitrate-reductases-in-hydrogenobacter-thermophilus-with-evolutionarily-ancient-features-distinctive-localization-and-electron-transfer
#19
Masafumi Kameya, Haruna Kanbe, Yasuo Igarashi, Hiroyuki Arai, Masaharu Ishii
Dissimilatory nitrate reductase (NAR) and assimilatory nitrate reductase (NAS) serve as key enzymes for nitrogen catabolism and anabolism in many organisms. We purified NAR and NAS from H. thermophilus, a hydrogen-oxidizing chemolithoautotroph belonging to the phylogenetically deepest branch in the Bacteria domain. Physiological contribution of these enzymes to nitrate respiration and assimilation was clarified by transcriptomic analysis and gene disruption experiments. These enzymes showed several features unreported in bacteria, such as the periplasmic orientation of NAR anchored with a putative transmembrane subunit and the specific electron transfer from a [4Fe-4S]-type ferredoxin to NAS...
July 27, 2017: Molecular Microbiology
https://www.readbyqxmd.com/read/28743354/analysis-of-four-serum-biomarkers-in-rheumatoid-arthritis-association-with-extra-articular-manifestations-in-patients-and-arthralgia-in-relatives
#20
Flávia R Nass, Thelma L Skare, Isabela Goeldner, Renato Nisihara, Iara T Messias-Reason, Shirley R R Utiyama
OBJECTIVES: To evaluate the frequency of four serum biomarkers in RA patients and their relatives and identify possible associations with clinical findings of the disease. METHODS: This was a transversal analytical study. Anti-cyclic citrullinated peptide (anti-CCP), anti-mutated citrullinated vimentin (anti-MCV) and IgA-rheumatoid factor (RF) were determined by ELISA and IgM-RF by latex agglutination in 210 RA patients, 198 relatives and 92 healthy controls from Southern Brazil...
July 2017: Revista Brasileira de Reumatologia
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